SitesBLAST

Comparing WP_083957205.1 NCBI__GCF_002835735.1:WP_083957205.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Found 13 hits to proteins with known functional sites

7jsjA Structure of the nact-pf2 complex (see paper)
40% identity, 83% coverage: 90:533/536 of query aligns to 33:465/468 of 7jsjA

• binding sodium ion: S124 (= S180), I127 (= I183), S128 (= S184), N129 (= N185), G172 (= G239), T366 (= T432), T369 (= T435), S370 (= S436), N371 (= N437), A413 (= A479), T414 (= T480)
• binding (2R)-2-[2-(4-tert-butylphenyl)ethyl]-2-hydroxybutanedioic acid: N129 (= N185), T130 (= T186), T173 (= T240), G315 (= G381), I316 (= I382), N371 (= N437)

8w6cA Cryoem structure of nadc1 with citrate (see paper)
38% identity, 85% coverage: 77:533/536 of query aligns to 33:522/530 of 8w6cA

• binding sodium ion: S136 (= S180), I139 (= I183), S140 (= S184), N141 (= N185), G191 (= G239), T423 (= T432), T426 (= T435), S427 (= S436), N428 (= N437), A470 (= A479)
• binding cholesterol hemisuccinate: Y37 (≠ L81), Y40 (≠ L84), I65 (≠ V110), M69 (≠ L114)

8w6gA Nadc1 with inhibitor aca (see paper)
38% identity, 85% coverage: 77:533/536 of query aligns to 33:519/527 of 8w6gA

• binding 2-[[(~{E})-3-(4-pentylphenyl)prop-2-enoyl]amino]benzoic acid: W233 (≠ Y281), L237 (≠ I285), A486 (≠ V498), R487 (≠ K499), F490 (= F502)
• binding cholesterol hemisuccinate: Y37 (≠ L81), Y40 (≠ L84), L66 (= L111), M69 (≠ L114)

8w6nA Nas1 with sulfate in in/out state (see paper)
40% identity, 77% coverage: 90:501/536 of query aligns to 41:459/494 of 8w6nA

• binding sodium ion: S132 (= S180), N137 (= N185), G181 (= G233), G187 (= G239), T188 (= T240)

8w6nB Nas1 with sulfate in in/out state (see paper)
39% identity, 77% coverage: 90:501/536 of query aligns to 37:447/481 of 8w6nB

• binding sulfate ion: N133 (= N185), T134 (= T186), T181 (= T240), S182 (≠ P241)

Q28615 Solute carrier family 13 member 2; Na(+)/dicarboxylate cotransporter 1; NaDC-1; Renal sodium/dicarboxylate cotransporter from Oryctolagus cuniculus (Rabbit) (see paper)
36% identity, 83% coverage: 90:533/536 of query aligns to 45:573/593 of Q28615

• L83 (≠ A127) mutation to C: Decreases cell membrane expression. Decreases succinate transport activity. Decreases Km value for succinate.
• T86 (= T130) mutation to C: Does not affect cell membrane localization. Decreases succinate transport activity.
• Y228 (= Y227) mutation to C: Does not affect cell membrane localization. Decreases succinate transport activity.
• Y432 (≠ L390) mutation to C: Does not affect cell membrane localization. Decreases succinate transport activity. Decreases Km value for succinate. More sensitive to inhibition by lithium.
• T474 (= T432) mutation to C: Does not affect cell membrane localization. Abolishes succinate transport activity.
• N525 (= N483) mutation to C: Decreases cell membrane expression. Decreases succinate transport activity.
• M539 (= M497) mutation to C: Does not affect cell membrane localization. Decreases succinate transport activity. Insensitive to inhibition by lithium.

Q86YT5 Na(+)/citrate cotransporter; NaCT; Sodium-coupled citrate transporter; Sodium-dependent citrate transporter; Solute carrier family 13 member 5 from Homo sapiens (Human) (see 5 papers)
36% identity, 83% coverage: 90:533/536 of query aligns to 45:559/568 of Q86YT5

• T142 (= T186) to M: in DEE25; no loss of localization to plasma membrane; loss of function in citrate transport; dbSNP:rs761917087
• G219 (= G232) to E: loss of localization to plasma membrane; loss of function in citrate transport; dbSNP:rs150024888; to R: in DEE25; loss of function in citrate transport; loss of localization to plasma membrane; dbSNP:rs144332569
• T227 (= T240) to M: in DEE25; loss of function in citrate transport; no effect on localization to plasma membrane; dbSNP:rs587777577
• D243 (≠ V256) to N: no effect on localization to plasma membrane; no effect on its function in citrate transport; dbSNP:rs142262032
• G409 (= G381) mutation to Q: No effect on its function in citrate transport.
• I410 (= I382) mutation I->A,F: Significant loss of function in citrate transport.; mutation to V: No effect on its function in citrate transport.
• L420 (≠ I392) to P: loss of localization to plasma membrane; loss of function in citrate transport; dbSNP:rs150738356
• S427 (= S399) to L: in DEE25; loss of localization to plasma membrane; loss of function in citrate transport; dbSNP:rs548065551
• L485 (≠ I457) to R: no effect on localization to plasma membrane; reduced function in citrate transport; increased Km and Vmax values compared with that of wild type with citrate as substrate; dbSNP:rs148049520
• L488 (≠ Y460) to P: in DEE25; loss of function in citrate transport; loss of localization to plasma membrane; dbSNP:rs587777578
• D524 (= D496) to H: in DEE25; loss of function in citrate transport; no effect on localization to plasma membrane; dbSNP:rs863225448

Sites not aligning to the query:

• 341:568 natural variant: Missing (in DEE25; loss of localization to plasma membrane; loss of function in citrate transport)
• 562 modified: carbohydrate, N-linked (GlcNAc...) asparagine

8w6hA Nas1 with sulfate - in/in state (see paper)
39% identity, 77% coverage: 90:501/536 of query aligns to 36:428/462 of 8w6hA

• binding cholesterol: I332 (= I405), P373 (= P446)
• binding sodium ion: S127 (= S180), L130 (≠ I183), S131 (= S184), N132 (= N185), G179 (= G239), T180 (= T240)
• binding sulfate ion: T133 (= T186), S181 (≠ P241)

7t9gA Structure of vcindy-na+ (see paper)
36% identity, 79% coverage: 89:509/536 of query aligns to 37:433/445 of 7t9gA

• binding sodium ion: S129 (= S180), S133 (= S184), N134 (= N185), G182 (= G239), T356 (= T432), A359 (≠ T435), S360 (= S436), N361 (= N437), A403 (= A479)

6wtxA Structure of vcindy in complex with terephthalate (see paper)
36% identity, 79% coverage: 89:509/536 of query aligns to 37:433/445 of 6wtxA