SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_084057564.1 NCBI__GCF_900176285.1:WP_084057564.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 5 hits to proteins with known functional sites (download)

P74535 Bifunctional arginine dihydrolase/ornithine cyclodeaminase ArgZ; EC 3.5.3.27; EC 4.3.1.12 from Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa) (see paper)
35% identity, 92% coverage: 23:363/371 of query aligns to 365:694/705 of P74535

query
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P74535

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Sites not aligning to the query:

22 binding L-arginine; binding L-ornithine; N→A: Significant loss of arginine dihydrolase activity.
65 D→A: Significant loss of arginine dihydrolase activity.
68 F→A: Significant loss of arginine dihydrolase activity.
71 binding L-arginine; binding L-ornithine; N→D: Produces equal trace amounts of citrulline and ornithine.; N→S: Transforms the enzyme from a dihydrolase to a deiminase.
90 binding L-arginine; binding L-ornithine; R→A: Significant loss of arginine dihydrolase activity.
118 E→A: Complete loss of arginine dihydrolase activity.
139 binding L-arginine; binding L-ornithine; R→A: Significant loss of arginine dihydrolase activity.
167 Y→A: Significant loss of arginine dihydrolase activity.
168 binding L-arginine; binding L-ornithine; H→F: Complete loss of arginine dihydrolase activity.
170 binding L-arginine
258 binding L-arginine; binding L-ornithine
264 binding covalent; binding L-ornithine; C→S: Complete loss of arginine dihydrolase activity.

6lrgA Crystal structure of the ternary complex of agre with ornithine and NAD+ (see paper)
32% identity, 91% coverage: 28:363/371 of query aligns to 369:673/678 of 6lrgA

query
sites
6lrgA

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binding nicotinamide-adenine-dinucleotide: P477 (≠ A157), V478 (= V158), G503 (= G183), N504 (= N184), A505 (= A185), H537 (= H217), S579 (= S259), R581 (= R261), D582 (= D262), D583 (= D263), S614 (≠ A294), S615 (≠ T295), M616 (≠ Q296), H618 (= H298), D636 (= D326), I637 (≠ M327), D659 (≠ N349), V660 (= V350)

Sites not aligning to the query:

binding L-ornithine: 20, 64, 67, 70, 89, 138, 166, 167, 263

Q8YMD9 Bifunctional arginine dihydrolase/ornithine cyclodeaminase AgrE; Arginine-guanidine removing enzyme; EC 3.5.3.27; EC 4.3.1.12 from Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) (see paper)
33% identity, 91% coverage: 28:363/371 of query aligns to 370:693/703 of Q8YMD9

query
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Q8YMD9

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N524 (= N184) binding NAD(+)
A525 (= A185) binding NAD(+)
D603 (= D263) binding NAD(+)
S635 (≠ T295) binding NAD(+)
M636 (≠ Q296) binding NAD(+)
L637 (= L297) binding NAD(+)
H638 (= H298) binding NAD(+)
D656 (= D326) binding NAD(+)
D679 (≠ N349) binding NAD(+)
V680 (= V350) binding NAD(+)

Sites not aligning to the query:

22 binding L-arginine; binding L-ornithine; N→A: Loss of arginine dihydrolase activity.
65 binding L-arginine; D→A: Shows residual arginine dihydrolase activity.
71 binding L-arginine; mutation N->A,D: Shows residual arginine dihydrolase activity.
90 binding L-arginine; binding L-ornithine; R→A: Loss of arginine dihydrolase activity.
118 E→A: Loss of arginine dihydrolase activity.
122 mutation D->A,N: Loss of arginine dihydrolase activity.
139 binding L-arginine; binding L-ornithine; R→A: Shows residual arginine dihydrolase activity.
167 Y→F: Retains 9% of arginine dihydrolase activity.
168 binding L-ornithine; H→A: Loss of arginine dihydrolase activity.
170 binding L-arginine; mutation D->A,N: Loss of arginine dihydrolase activity.
219 N→A: Loss of arginine dihydrolase activity.
258 binding L-arginine
264 binding L-ornithine; C→A: Loss of arginine dihydrolase activity.

6lrgB Crystal structure of the ternary complex of agre with ornithine and NAD+ (see paper)
32% identity, 91% coverage: 28:363/371 of query aligns to 367:679/681 of 6lrgB

query
sites
6lrgB

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E

E

T

R

S

R

F

V

S

E

I

L

D

V

Y

V

D

E

E

Q

F

V

Y

A

D

W

L

E

L

L

V

R

H

K

E

I

R

R

N

D

H

A

G

G

T

G

I

K

V

V

W

V

V

V

L

T

G

A

P

G

A

P

V

V

F

I

D

H

Q

T

D

G

A

G

R

G

S

E

A

H

F

L

S

S

S

R

L

L

I

I

R

R

R

E

G

G

Y

Y

V

V

H

Q

A

A

L

L

A

G

G

G

N

N

A

A

L

I

A

A

T

V

H

H

D

D

L

I

E

E

G

Q

A

N

L

M

F

M

G

G

T

T

A

S

L

L

G

G

Q

V

E

D

I

M

Y

K

S

R

K

G

R

V

S

A

A

V

P

R

L

G

G

G

H

H

Y

R

H

H

L

L

D

K

A

V

I

I

N

N

R

T

L

I

R

R

E

R

L

H

G

G

S

S

I

I

E

A

E

K

A

G

M

V

Q

E

R

S

G

G

V

I

T

R

D

S

G

G

V

V

M

M

H

Y

A

E

A

C

V

V

T

R

Q

N

G

Q

I

I

P

P

V

F

V

V

L

L

A

A

G

G

S

S

I

I

R

R

D

D

G

G

P

P

L

L

P

P

G

D

V

T

I

Q

A

M

D

D

V

L

Y

I

E

K

A

A

Q

Q

D

E

R

E

M

Y

R

A

D

K

Q

H

V

L

R

E

R

G

A

A

T

E

T

M

V

I

V

L

A

M

L

L

A

S

T

S

Q

M

L

L

H

H

A

S

I

I

A

G

T

V

G

G

N

N

M

M

A

T

P

P

S

A

Y

-

R

-

V

-

L

-

D

-

G

-

T

-

V

-

R

-

P

G

V

V

Y

K

F

M

Y

V

S

C

V

V

D

D

M

I

S

N

E

P

F

A

A

V

V

V

Q

T

K

K

L

L

A

S

N

D

R

R

G

G

S

S

L

I

T

E

A

S

R

V

S

G

I

V

L

V

T

T

N

D

V

V

Q

G

D

L

F

F

M

L

V

S

T

L

V

L

E

T

R

Q

G

Q

L

L

R

D

R

K

Sites not aligning to the query:

binding L-ornithine: 18, 62, 65, 68, 87, 136, 164, 165, 255, 261

6lrhA Crystal structure of the binary complex of agre c264a mutant with l- arginine (see paper)
32% identity, 91% coverage: 28:363/371 of query aligns to 369:671/675 of 6lrhA

query
sites
6lrhA

V

V

R

I

K

Q

A

D

G

G

V

V

A

A

P

P

E

D

G

D

Y

F

H

Y

A

V

T

S

S

T

V

I

F

Y

P

P

E

T

Y

E

F

V

H

R

V

I

A

N

P

-

G

G

R

Q

W

W

E

I

I

K

L

V

R

E

E

N

S

Q

R

R

M

M

D

D

-

G

-

A

C

I

V

A

V

I

V

T

Q

Q

R

T

P

P

D

N

G

G

R

L

L

L

D

A

V

Q

V

C

E

K

-

I

F

L

R

R

R

D

L

L

Q

K

P

A

G

G

D

E

R

Q

V

V

A

I

V

V

G

-

R

-

E

-

N

-

A

-

E

-

G

-

I

-

F

-

V

-

H

-

A

-

E

-

A

-

F

-

Q

-

G

-

M

-

S

-

G

-

T

-

G

-

D

D

K

V

F

L

A

G

F

I

R

R

T

T

-

I

R

R

L

V

T

S

R

S

E

E

T

R

S

R

F

V

S

E

I

L

D

V

Y

V

D

E

E

Q

F

V

Y

A

D

W

L

E

L

L

V

R

H

K

E

I

R

R

N

D

H

A

G

G

T

G

I

K

V

V

W

V

V

V

L

T

G

A

P

G

A

P

V

V

F

I

D

H

Q

T

D

G

A

G

R

G

S

E

A

H

F

L

S

S

S

R

L

L

I

I

R

R

R

E

G

G

Y

Y

V

V

H

Q

A

A

L

L

A

G

G

G

N

N

A

A

L

I

A

A

T

V

H

H

D

D

L

I

E

E

G

Q

A

N

L

M

F

M

G

G

T

T

A

S

L

L

G

G

Q

V

E

D

I

M

Y

K

S

R

K

G

R

V

S

A

A

V

P

R

L

G

G

G

H

H

Y

R

H

H

L

L

D

K

A

V

I

I

N

N

R

T

L

I

R

R

E

R

L

H

G

G

S

S

I

I

E

A

E

K

A

G

M

V

Q

E

R

S

G

G

V

I

T

R

D

S

G

G

V

V

M

M

H

Y

A

E

A

C

V

V

T

R

Q

N

G

Q

I

I

P

P

V

F

V

V

L

L

A

A

G

G

S

S

I

I

R

R

D

D

G

G

P

P

L

L

P

P

G

D

V

T

I

Q

A

M

D

D

V

L

Y

I

E

K

A

A

Q

Q

D

E

R

E

M

Y

R

A

D

K

Q

H

V

L

R

E

R

G

A

A

T

E

T

M

V

I

V

L

A

M

L

L

A

S

T

S

Q

M

L

L

H

H

A

S

I

I

A

G

T

V

G

G

N

N

M

M

A

T

P

P

S

A

Y

-

R

-

V

-

L

-

D

-

G

-

T

-

V

-

R

-

P

G

V

V

Y

K

F

M

Y

V

S

C

V

V

D

D

M

I

S

N

E

-

F

-

A

P

V

A

Q

T

K

K

L

L

A

S

N

D

R

R

G

G

S

S

L

I

T

E

A

S

R

V

S

G

I

V

L

V

T

T

N

D

V

V

Q

G

D

L

F

F

M

L

V

S

T

L

V

L

E

T

R

Q

G

Q

L

L

R

D

R

K

Sites not aligning to the query:

binding arginine: 20, 64, 67, 70, 89, 138, 166, 167, 169, 257, 263

Query Sequence

>WP_084057564.1 NCBI__GCF_900176285.1:WP_084057564.1
MFRLPQYSPPDFSDPRFAQAPLVTFREVRKAGVAPEGYHATSVFPEYFHVAPGRWEILRE
SRMDCVVVQRPDGRLDVVEFRRLQPGDRVAVGRRENAEEGIFVHAEAFQGMSGTGDKFAF
RTRLTRETSFSIDYDEFYDLLVHERNHGTIVWVLGPAVVFDQDARSAFSSLIRRGYVHAL
LAGNALATHDLEGALFGTALGQEIYSKRSAPLGHYHHLDAINRLRELGSIEEAMQRGVVT
DGVMHAAVTQGIPVVLAGSIRDDGPLPGVIADVYEAQDRMRDQVRRATTVVALATQLHAI
ATGNMAPSYRVLDDGTVRPVYFYSVDMSEFAVQKLANRGSLTARSILTNVQDFMVTVERG
LRRRETNEEIA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory