SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_084076347.1 NCBI__GCF_900129305.1:WP_084076347.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P10880 Shikimate kinase 2; SK 2; Shikimate kinase II; SKII; EC 2.7.1.71 from Dickeya chrysanthemi (Pectobacterium chrysanthemi) (Erwinia chrysanthemi) (see paper)
39% identity, 77% coverage: 34:196/213 of query aligns to 5:165/173 of P10880

query
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P10880

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C13 (≠ T42) mutation to S: 66% of wild-type activity. Increase in substrates affinity.
K15 (= K44) mutation to M: Loss of activity. Increased thermostability.
D34 (= D63) mutation to N: Loss of activity.
C162 (≠ A193) mutation to S: No effect on activity and substrates affinity.

1e6cA K15m mutant of shikimate kinase from erwinia chrysanthemi (see paper)
38% identity, 77% coverage: 34:196/213 of query aligns to 5:165/170 of 1e6cA

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1e6cA

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binding phosphate ion: R11 (= R40), G12 (= G41), M15 (≠ K44), T16 (≠ S45)

2shkB The three-dimensional structure of shikimate kinase from erwinia chrysanthemi complexed with adp (see paper)
37% identity, 77% coverage: 34:196/213 of query aligns to 5:155/162 of 2shkB

query
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binding adenosine-5'-diphosphate: G12 (= G41), C13 (≠ T42), G14 (= G43), K15 (= K44), T16 (≠ S45), T17 (= T46), R110 (= R151), Q145 (≠ S186), P147 (≠ S188)
binding magnesium ion: Q134 (≠ R175), A137 (= A178)

4y0aA Shikimate kinase from acinetobacter baumannii in complex with shikimate (see paper)
32% identity, 78% coverage: 30:196/213 of query aligns to 7:169/179 of 4y0aA

query
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binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: M17 (≠ R40), D40 (= D63), F63 (= F86), R64 (= R87), G86 (= G109), G87 (= G110), L126 (≠ S153), R143 (= R170)
binding sulfate ion: G18 (= G41), A19 (≠ T42), G20 (= G43), K21 (= K44), T22 (≠ S45), E112 (≠ P135), R124 (= R151), R137 (≠ S164)

1shkA The three-dimensional structure of shikimate kinase from erwinia chrysanthemi (see paper)
34% identity, 77% coverage: 34:196/213 of query aligns to 5:150/158 of 1shkA

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binding magnesium ion: R92 (= R122), A93 (≠ D123), G95 (≠ F125), Q129 (≠ R175), A132 (= A178)

7tbvA Crystal structure of the shikimate kinase + 3-dehydroquinate dehydratase + 3-dehydroshikimate dehydrogenase domains of aro1 from candida albicans (see paper)
43% identity, 41% coverage: 29:115/213 of query aligns to 1:89/682 of 7tbvA

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Sites not aligning to the query:

binding magnesium ion: 300, 302, 329

P07547 Pentafunctional AROM polypeptide; EC 4.2.3.4; EC 2.5.1.19; EC 2.7.1.71; EC 4.2.1.10; EC 1.1.1.25 from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) (see 2 papers)
35% identity, 69% coverage: 34:180/213 of query aligns to 866:1009/1583 of P07547

query
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P07547

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Sites not aligning to the query:

44:46 binding
81:84 binding
114:116 binding
119 binding
139:140 binding
161 binding
179:182 binding
190 binding
194 binding
271 binding
287 binding

3n2eA Crystal structure of helicobactor pylori shikimate kinase in complex with nsc162535 (see paper)
30% identity, 78% coverage: 31:197/213 of query aligns to 1:162/168 of 3n2eA

query
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-

N

N

G

L

K

K

I

G

L

L

R

G

D

T

C

T

F

F

H

-

V

-

W

Y

L

L

D

K

C

M

D

D

L

F

P

E

V

T

I

L

L

I

Q

K

R

R

L

-

A

-

L

L

D

N

P

Q

K

K

T

E

A

R

T

A

Q

K

R
|
R

P

P

S

L

L

L

T

N

G

N

R

L

H

-

V

-

Q

-

D

T

E

Q

T

A

S

K

Q

E

I

L

L

F

E

E

R

K

R
|
R

R

Q

P

A

L
|
L

Y
|
Y

R

E

V

K

I

N

A

A

H

S

V

F

A

I

V

I

D

D

S

A

-

R

G

G

S

G

L

L

-

N

S

S

A

L

E

K

E

Q

I

V

A

L

E

Q

V

F

I

I

A

A

binding 7-amino-4-hydroxy-3-[(E)-(5-hydroxy-7-sulfonaphthalen-2-yl)diazenyl]naphthalene-2-sulfonic acid: F9 (≠ Y39), R45 (≠ A75), F48 (≠ V78), R57 (= R87), G80 (= G109), G81 (= G110), M84 (≠ E113), R116 (= R151), R132 (= R170), L135 (= L173), Y136 (= Y174)

3mufA Shikimate kinase from helicobacter pylori in complex with shikimate-3- phosphate and adp (see paper)
30% identity, 72% coverage: 32:184/213 of query aligns to 1:145/160 of 3mufA

query
sites
3mufA

Q

Q

R

H

I

L

A

V

L

L

I

I

G

G

Y

F

R
x
M

G
|
G

T
x
S

G
|
G

K
|
K

S
|
S

T
x
S

V

L

G

A

D

Q

L

E

L

L

A

G

A

L

R

A

L

L

G

K

W

L

D

E

F

V

V

L

D

D

M

T

D
|
D

R

M

V

I

L

I

T

S

Q

E

R

R

F

V

G

G

T

L

S

S

I

V

A

R

H

E

F

I

V

F

A

E

Q

E

N

L

G

G

W

E

E

D

A

N

F

F

R
|
R

E

M

A

F

E

E

S

K

A

N

L

L

L

I

G

D

Q

E

I

L

-

K

S

T

R

L

E

K

E

T

G

P

L

H

V

V

V

I

A

S

T

T

G
|
G

I

I

V

V

E

M

K

H

E

E

R

-

N

N

G

L

K

K

I

G

L

L

R

G

D

T

C

T

F

F

H

-

V

-

W

Y

L

L

D

K

C
x
M

D

D

L

F

P

E

V

T

I

L

L

I

Q

K

R
|
R

L

-

A

-

L

L

D

N

P

Q

K

K

T

E

A

R

T

E

Q

K

R
|
R

P

P

S

L

L

L

T

N

G

N

R

L

H

-

V

-

Q

-

D

T

E

Q

T

A

S

K

Q

E

I

L

L

F

E

E

R

K

R
|
R

R

Q

P

A

L

L

Y

Y

R

E

V

K

I

N

A

A

H

S

V

F

A

I

V

I

D

D

S

A

binding adenosine-5'-diphosphate: G10 (= G41), S11 (≠ T42), G12 (= G43), K13 (= K44), S14 (= S45), S15 (≠ T46), M98 (≠ C132), R106 (= R140)
binding shikimate-3-phosphate: M9 (≠ R40), D32 (= D63), R56 (= R87), G78 (= G108), G79 (= G109), G80 (= G110), R115 (= R151), R131 (= R170)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 147, 148

6hqvA Pentafunctional arom complex from chaetomium thermophilum (see paper)
33% identity, 68% coverage: 30:174/213 of query aligns to 841:983/1555 of 6hqvA

query
sites
6hqvA

T

T

I

D

Q

R

R

S

I

I

A

F

L

I

I

V

G

G

Y

M

R

R

G

G

T

A

G

G

K

K

S

S

T

T

V

A

G

G

D

R

L

W

L

M

A

S

A

E

R

L

L

L

G

K

W

R

D

P

F

L

V

V

D

D

M

L

D
|
D

R

A

V

E

L

L

T

E

Q

R

R

R

F

E

G

G

T

M

S

T

I

I

A

P

H

E

F

I

V

I

-

R

A

G

Q

E

N

R

G

G

W

W

E

E

A

G

F

F

R

R

E

Q

A

A

E

E

S

L

A

E

L

L

G

Q

Q

D

I

V

-

I

-

K

S

N

R

Q

E

S

E

K

G

G

L

Y

V

I

V

F

A

S

T

C

G

G

G
|
G

I

I

V

V

E

E

K

T

E

E

R

A

N

A

G

R

K

K

I

L

L

L

R

I

D

D

C

-

F

Y

H

H

-

K

-

N

-

G

-

P

V

V

V

L

W

L

L

V

D

H

C

R

D

D

L

T

P

D

V

Q

I

V

L

V

Q

E

R

Y

L

L

A

M

L

R

D

D

P

-

K

K

T

T

A

-

T

-

Q

-

R

R

P

P

S

A

L

Y

T

S

G

-

R

-

H

-

V

-

Q

-

D

E

E

N

T

I

S

R

Q

E

I

V

L

Y

E

E

R

R

R
|
R

R

K

P

P

L

W

Y

F

binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D874 (= D63), G923 (= G109), G924 (= G110), R979 (= R170)

Sites not aligning to the query:

active site: 123, 145, 187, 243, 253, 257, 261, 264, 268, 280
binding (4S,5R)-4,5-dihydroxy-3-oxocyclohex-1-ene-1-carboxylic acid: 1060, 1062, 1181, 1224, 1232, 1242, 1243
binding glutamic acid: 139, 145, 187, 243, 257, 264, 280
binding nicotinamide-adenine-dinucleotide: 42, 44, 45, 76, 79, 107, 108, 109, 112, 132, 133, 135, 139, 140, 145, 154, 175, 176, 177, 180, 280
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: 413, 562, 563, 1277, 1279, 1323, 1327, 1348, 1368, 1526
binding zinc ion: 187, 264, 280

1zuiA Structural basis for shikimate-binding specificity of helicobacter pylori shikimate kinase (see paper)
30% identity, 72% coverage: 32:184/213 of query aligns to 1:143/158 of 1zuiA

query
sites
1zuiA

Q

Q

R

H

I

L

A

V

L

L

I

I

G

G

Y

F

R
x
M

G

G

T

S

G

G

K

K

S

S

T

S

V

L

G

A

D

Q

L

E

L

L

A

G

A

L

R

A

L

L

G

K

W

L

D

E

F

V

V

L

D

D

M

T

D
|
D

R

M

V

I

L

I

T

S

Q

E

R

R

F

V

G

G

T

L

S

S

I

V

A

R

H

E

F

I

V
x
F

A

E

Q

E

N

L

G

G

W

E

E

D

A

N

F

F

R
|
R

E

M

A

F

E

E

S

K

A

N

L

L

L

I

G

D

Q

E

I

L

-

K

S

T

R

L

E

K

E

T

G

P

L

H

V

V

V

I

A

S

T

T

G
|
G

G

G

I

I

V

V

E

M

K

H

E

E

R

-

N

N

G

L

K

K

I

G

L

L

R

G

D

T

C

T

F

F

H

-

V

-

W

Y

L

L

D

K

C

M

D

D

L

F

P

E

V

T

I

L

L

I

Q

K

R

R

L

L

A

N

L

Q

D

R

P
x
E

K

K

T

-

A

-

T

-

Q

-

R
|
R

P

P

S

L

L

L

T

N

G

N

R

L

H

-

V

-

Q

-

D

T

E

Q

T

A

S

K

Q

E

I

L

L

F

E

E

R

K

R
|
R

R

Q

P

A

L

L

Y

Y

R

E

V

K

I

N

A

A

H

S

V

F

A

I

V

I

D

D

S

A

binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: M9 (≠ R40), D32 (= D63), F47 (≠ V78), R56 (= R87), G78 (= G108), G79 (= G109), E111 (≠ P145), R113 (= R151), R129 (= R170)

2iyvA Shikimate kinase from mycobacterium tuberculosis in complex with adp, open lid (conf. B) (see paper)
32% identity, 70% coverage: 36:184/213 of query aligns to 6:149/179 of 2iyvA

query
sites
2iyvA

L

L

I

V

G

G

Y

L

R

P

G
|
G

T

S

G
|
G

K
|
K

S
|
S

T
|
T

V

I

G

G

D

R

L

R

L

L

A

A

A

K

R

A

L

L

G

G

W

V

D

G

F

L

V

L

D

D

M

T

D

D

R

V

V

A

L

I

T

E

Q

Q

R

R

F

T

G

G

T

R

S

S

I

I

A

A

H

D

F

I

V

F

A

A

Q

T

N

D

G

G

W

E

E

Q

A

E

F

F

R

R

E

R

A

I

E

E

S

E

A

D

L

V

G

R

Q

A

I

A

S

L

R

A

E

D

E

H

G

D

L

G

V

V

V

L

A

S

T

L

G

G

I

A

V

V

E

T

K

S

E

P

R

G

N

V

G

R

K

A

I

A

L

L

R

-

D

A

C

G

F

H

H

T

V

V

W

Y

L

L

D

E

C

I

D

S

L

A

P

A

V

E

I

G

L

V

Q

R

R
|
R

L

T

A

G

L

G

D

N

P

-

K

-

T

-

A

-

T

T

Q

V

R

R

P

P

S

L

L

L

T

A

G

G

R

P

H

D

V

R

Q

A

D

E

E

K

T

Y

S

R

Q

A

I

L

L

M

E

A

R

K

R

R

R

A

P

P

L

L

Y

Y

R

R

V

R

I

V

A

A

H

T

V

M

A

R

V

V

D

D

S

T

binding adenosine-5'-diphosphate: G11 (= G41), G13 (= G43), K14 (= K44), S15 (= S45), T16 (= T46), R109 (= R140)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 152, 154

2iywA Shikimate kinase from mycobacterium tuberculosis in complex with mgatp, open lid (conf. B) (see paper)
32% identity, 70% coverage: 36:184/213 of query aligns to 6:149/178 of 2iywA

query
sites
2iywA

L

L

I

V

G

G

Y

L

R

P

G
|
G

T

S

G
|
G

K
|
K

S
|
S

T
|
T

V

I

G

G

D

R

L

R

L

L

A

A

A

K

R

A

L

L

G

G

W

V

D

G

F

L

V

L

D

D

M

T

D

D

R

V

V

A

L

I

T

E

Q

Q

R

R

F

T

G

G

T

R

S

S

I

I

A

A

H

D

F

I

V

F

A

A

Q

T

N

D

G

G

W

E

E

Q

A

E

F

F

R

R

E

R

A

I

E

E

S

E

A

D

L

V

G

R

Q

A

I

A

S

L

R

A

E

D

E

H

G

D

L

G

V

V

V

L

A

S

T

L

G

G

I

A

V

V

E

T

K

S

E

P

R

G

N

V

G

R

K

A

I

A

L

L

R

-

D

A

C

G

F

H

H

T

V

V

W

Y

L

L

D

E

C

I

D

S

L

A

P

A

V

E

I

G

L

V

Q

R

R
|
R

L

T

A

G

L

G

D

N

P

-

K

-

T

-

A

-

T

T

Q

V

R

R

P

P

S

L

L

L

T

A

G

G

R

P

H

D

V

R

Q

A

D

E

E

K

T

Y

S

R

Q

A

I

L

L

M

E

A

R

K

R

R

R

A

P

P

L

L

Y

Y

R

R

V

R

I

V

A

A

H

T

V

M

A

R

V

V

D

D

S

T

binding adenosine-5'-triphosphate: G11 (= G41), G13 (= G43), K14 (= K44), S15 (= S45), T16 (= T46), R109 (= R140)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 152, 154

3bafA Crystal structure of shikimate kinase from mycobacterium tuberculosis in complex with amp-pnp
32% identity, 70% coverage: 36:184/213 of query aligns to 6:149/165 of 3bafA

query
sites
3bafA

L

L

I

V

G

G

Y

L

R
x
P

G

G

T

S

G
|
G

K
|
K

S
|
S

T
|
T

V

I

G

G

D

R

L

R

L

L

A

A

A

K

R

A

L

L

G

G

W

V

D

G

F

L

V

L

D

D

M

T

D
|
D

R

V

V

A

L

I

T

E

Q

Q

R

R

F

T

G

G

T

R

S

S

I

I

A

A

H

D

F

I

V

F

A

A

Q

T

N

D

G

G

W

E

E

Q

A

E

F

F

R

R

E

R

A

I

E

E

S

E

A

D

L

V

G

R

Q

A

I

A

S

L

R

A

E

D

E

H

G

D

L

G

V

V

V

L

A

S

T

L

G

G

I

A

V

V

E

T

K

S

E

P

R

G

N

V

G

R

K

A

I

A

L

L

R

-

D

A

C

G

F

H

H

T

V

V

W

Y

L

L

D

E

C

I

D

S

L

A

P

A

V

E

I

G

L

V

Q

R

R
|
R

L

T

A

G

L

G

D

N

P

-

K

-

T

-

A

-

T

T

Q

V

R
|
R

P

P

S

L

L

L

T

A

G

G

R

P

H

D

V

R

Q

A

D

E

E

K

T

Y

S

R

Q

A

I

L

L

M

E

A

R

K

R
|
R

R

A

P

P

L

L

Y

Y

R

R

V

R

I

V

A

A

H

T

V

M

A

R

V

V

D

D

S

T

binding phosphoaminophosphonic acid-adenylate ester: P10 (≠ R40), G13 (= G43), K14 (= K44), S15 (= S45), T16 (= T46), R109 (= R140), R116 (= R151)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D33 (= D63), R135 (= R170)

2dfnA Structure of shikimate kinase from mycobacterium tuberculosis complexed with adp and shikimate at 1.9 angstrons of resolution (see paper)
32% identity, 70% coverage: 36:184/213 of query aligns to 6:149/165 of 2dfnA

query
sites
2dfnA

L

L

I

V

G

G

Y

L

R
x
P

G
|
G

T
x
S

G
|
G

K
|
K

S
|
S

T
|
T

V

I

G

G

D

R

L

R

L

L

A

A

A

K

R

A

L

L

G

G

W

V

D

G

F

L

V

L

D

D

M

T

D
|
D

R

V

V

A

L

I

T

E

Q

Q

R

R

F

T

G

G

T

R

S

S

I
|
I

A

A

H

D

F

I

V

F

A

A

Q

T

N

D

G

G

W

E

E

Q

A

E

F
|
F

R
|
R

E

R

A

I

E

E

S

E

A

D

L

V

G

R

Q

A

I

A

S

L

R

A

E

D

E

H

G

D

L

G

V

V

V

L

A

S

T

L

G
|
G

I

A

V

V

E

T

K

S

E

P

R

G

N

V

G

R

K

A

I

A

L

L

R

-

D

A

C

G

F

H

H

T

V

V

W

Y

L

L

D

E

C

I

D

S

L

A

P

A

V

E

I

G

L

V

Q

R

R
|
R

L

T

A

G

L

G

D

N

P

-

K

-

T

-

A

-

T

T

Q

V

R
|
R

P

P

S

L

L

L

T

A

G

G

R

P

H

D

V

R

Q

A

D

E

E

K

T

Y

S

R

Q

A

I

L

L

M

E

A

R

K

R
|
R

R

A

P

P

L

L

Y

Y

R

R

V

R

I

V

A

A

H

T

V

M

A

R

V

V

D

D

S

T

binding adenosine-5'-diphosphate: G11 (= G41), S12 (≠ T42), G13 (= G43), K14 (= K44), S15 (= S45), T16 (= T46), R109 (= R140), R116 (= R151)
binding chloride ion: P10 (≠ R40), K14 (= K44), G79 (= G109)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D33 (= D63), I44 (= I74), F56 (= F86), R57 (= R87), G78 (= G108), G79 (= G109), G80 (= G110), R135 (= R170)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 152, 154

1we2A Crystal structure of shikimate kinase from mycobacterium tuberculosis in complex with mgadp and shikimic acid (see paper)
32% identity, 70% coverage: 36:184/213 of query aligns to 6:149/165 of 1we2A

query
sites
1we2A

L

L

I

V

G

G

Y

L

R
x
P

G
|
G

T

S

G
|
G

K
|
K

S
|
S

T
|
T

V

I

G

G

D

R

L

R

L

L

A

A

A

K

R

A

L

L

G

G

W

V

D

G

F

L

V

L

D

D

M

T

D
|
D

R

V

V

A

L

I

T

E

Q

Q

R

R

F

T

G

G

T

R

S

S

I
|
I

A

A

H

D

F

I

V
x
F

A

A

Q

T

N

D

G

G

W

E

E

Q

A

E

F

F

R

R

E

R

A

I

E

E

S

E

A

D

L

V

G

R

Q

A

I

A

S

L

R

A

E

D

E

H

G

D

L

G

V

V

V

L

A

S

T

L

G
|
G

I

A

V

V

E

T

K

S

E

P

R

G

N

V

G

R

K

A

I

A

L

L

R

-

D

A

C

G

F

H

H

T

V

V

W

Y

L

L

D

E

C

I

D

S

L

A

P

A

V

E

I

G

L

V

Q

R

R
|
R

L

T

A

G

L

G

D

N

P

-

K

-

T

-

A

-

T

T

Q

V

R
|
R

P

P

S
x
L

L

L

T

A

G

G

R

P

H

D

V

R

Q

A

D

E

E

K

T

Y

S

R

Q

A

I

L

L

M

E

A

R

K

R
|
R

R

A

P

P

L

L

Y

Y

R

R

V

R

I

V

A

A

H

T

V

M

A

R

V

V

D

D

S

T

binding adenosine-5'-diphosphate: P10 (≠ R40), G11 (= G41), G13 (= G43), K14 (= K44), S15 (= S45), T16 (= T46), R109 (= R140), R116 (= R151)
binding 3-dehydroshikimate: D33 (= D63), I44 (= I74), F48 (≠ V78), G78 (= G108), G79 (= G109), G80 (= G110), L118 (≠ S153), R135 (= R170)

1zyuA Crystal structure of mycobacterium tuberculosis shikimate kinase in complex with shikimate and amppcp at 2.85 angstrom resolution (see paper)
32% identity, 70% coverage: 36:184/213 of query aligns to 6:149/168 of 1zyuA

query
sites
1zyuA

L

L

I

V

G

G

Y

L

R
x
P

G
|
G

T

S

G
|
G

K
|
K

S
|
S

T
|
T

V

I

G

G

D

R

L

R

L

L

A

A

A

K

R

A

L

L

G

G

W

V

D

G

F

L

V

L

D

D

M

T

D
|
D

R

V

V

A

L

I

T

E

Q

Q

R

R

F

T

G

G

T

R

S

S

I

I

A

A

H

D

F

I

V

F

A

A

Q

T

N

D

G

G

W

E

E

Q

A

E

F

F

R
|
R

E

R

A

I

E

E

S

E

A

D

L

V

G

R

Q

A

I

A

S

L

R

A

E

D

E

H

G

D

L

G

V

V

V

L

A

S

T

L

G

G

G
|
G

I

A

V

V

E

T

K

S

E

P

R

G

N

V

G

R

K

A

I

A

L

L

R

-

D

A

C

G

F

H

H

T

V

V

W

Y

L

L

D

E

C

I

D

S

L

A

P

A

V

E

I

G

L

V

Q

R

R
|
R

L

T

A

G

L

G

D

N

P

-

K

-

T

-

A

-

T

T

Q

V

R

R

P
|
P

S
x
L

L

L

T

A

G

G

R

P

H

D

V

R

Q

A

D

E

E

K

T

Y

S

R

Q

A

I

L

L

M

E

A

R

K

R
|
R

R

A

P

P

L

L

Y

Y

R

R

V

R

I

V

A

A

H

T

V

M

A

R

V

V

D

D

S
x
T

binding phosphomethylphosphonic acid adenylate ester: P10 (≠ R40), G11 (= G41), G13 (= G43), K14 (= K44), S15 (= S45), T16 (= T46), R109 (= R140), T149 (≠ S184)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D33 (= D63), R57 (= R87), G80 (= G110), P117 (= P152), L118 (≠ S153), R135 (= R170)

Sites not aligning to the query:

binding phosphomethylphosphonic acid adenylate ester: 152, 154

4bqsA Crystal structure of mycobacterium tuberculosis shikimate kinase in complex with adp and a shikimic acid derivative. (see paper)
32% identity, 70% coverage: 36:184/213 of query aligns to 6:149/169 of 4bqsA

query
sites
4bqsA

L

L

I

V

G

G

Y

L

R
x
P

G
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G

T

S

G
|
G

K
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K

S
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S

T
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T

V

I

G

G

D

R

L

R

L

L

A

A

A

K

R

A

L

L

G

G

W

V

D

G

F

L

V

L

D

D

M

T

D
|
D

R

V

V

A

L

I

T

E

Q

Q

R

R

F

T

G

G

T

R

S

S

I

I

A

A

H

D

F

I

V

F

A

A

Q

T

N

D

G

G

W

E

E

Q

A

E

F

F

R

R

E

R

A

I

E

E

S

E

A

D

L

V

G

R

Q

A

I

A

S

L

R

A

E

D

E

H

G

D

L

G

V

V

V

L

A

S

T

L

G

G

I

A

V

V

E

T

K

S

E

P

R

G

N

V

G

R

K

A

I

A

L

L

R

-

D

A

C

G

F

H

H

T

V

V

W

Y

L

L

D

E

C

I

D

S

L

A

P

A

V

E

I

G

L

V

Q

R

R
|
R

L

T

A

G

L

G

D

N

P

-

K

-

T

-

A

-

T

T

Q

V

R

R

P

P

S

L

L

L

T

A

G

G

R

P

H

D

V

R

Q

A

D

E

E

K

T

Y

S

R

Q

A

I

L

L

M

E

A

R

K

R
|
R

R

A

P

P

L

L

Y

Y

R

R

V

R

I

V

A

A

H

T

V

M

A

R

V

V

D

D

S

T

binding adenosine-5'-diphosphate: P10 (≠ R40), G11 (= G41), G13 (= G43), K14 (= K44), S15 (= S45), T16 (= T46), R109 (= R140)
binding (1R,6R,10S)-6,10-dihydroxy-2-oxabicyclo[4.3.1]deca-4(Z),7-diene-8-carboxylic acid: D33 (= D63), R135 (= R170)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 152, 154

2iyyA Shikimate kinase from mycobacterium tuberculosis in complex with shikimate-3-phosphate and so4 (see paper)
32% identity, 70% coverage: 36:184/213 of query aligns to 6:149/169 of 2iyyA

query
sites
2iyyA

L

L

I

V

G

G

Y

L

R

P

G

G

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G

G

K
|
K

S

S

T

T

V

I

G

G

D

R

L

R

L

L

A

A

A

K

R

A

L

L

G

G

W

V

D

G

F

L

V

L

D

D

M

T

D
|
D

R

V

V

A

L

I

T
x
E

Q

Q

R

R

F

T

G

G

T

R

S
|
S

I
|
I

A

A

H

D

F

I

V

F

A

A

Q

T

N

D

G

G

W

E

E

Q

A

E

F

F

R
|
R

E

R

A

I

E

E

S

E

A

D

L

V

G

R

Q

A

I

A

S

L

R

A

E

D

E

H

G

D

L

G

V

V

V

L

A

S

T

L

G

G

G
|
G

I

A

V

V

E

T

K

S

E

P

R

G

N

V

G

R

K

A

I

A

L

L

R

-

D

A

C

G

F

H

H

T

V

V

W

Y

L

L

D

E

C

I

D

S

L

A

P

A

V

E

I

G

L

V

Q

R

R

R

L

T

A

G

L

G

D

N

P

-

K

-

T

-

A

-

T

T

Q

V

R

R

P

P

S
x
L

L

L

T

A

G

G

R

P

H

D

V

R

Q

A

D

E

E

K

T

Y

S

R

Q

A

I

L

L

M

E

A

R

K

R
|
R

R

A

P

P

L

L

Y

Y

R

R

V

R

I

V

A

A

H

T

V

M

A

R

V

V

D

D

S

T

binding magnesium ion: E37 (≠ T67), S43 (= S73), I44 (= I74)
binding phosphate ion: K14 (= K44), G79 (= G109)
binding shikimate-3-phosphate: K14 (= K44), D33 (= D63), R57 (= R87), G79 (= G109), G80 (= G110), L118 (≠ S153), R135 (= R170)

1u8aA Crystal structure of mycobacterium tuberculosis shikimate kinase in complex with shikimate and adp at 2.15 angstrom resolution (see paper)
32% identity, 70% coverage: 36:184/213 of query aligns to 6:147/163 of 1u8aA

query
sites
1u8aA

L

L

I

V

G

G

Y

L

R

P

G
|
G

T

S

G
|
G

K
|
K

S
|
S

T
|
T

V

I

G

G

D

R

L

R

L

L

A

A

A

K

R

A

L

L

G

G

W

V

D

G

F

L

V

L

D

D

M

T

D
|
D

R

V

V

A

L

I

T

E

Q

Q

R

R

F

T

G

G

T

R

S

S

I

I

A

A

H

D

F

I

V
x
F

A

A

Q

T

N

D

G

G

W

E

E

Q

A

E

F

F

R
|
R

E

R

A

I

E

E

S

E

A

D

L

V

G

R

Q

A

I

A

S

L

R

A

E

D

E

H

G

D

L

G

V

V

V

L

A

S

T

L

G

G

G
|
G

I

A

V

V

E

T

K

S

E

P

R

G

N

V

G

R

K

A

I

A

L

L

R

-

D

A

C

G

F

H

H

T

V

V

W

Y

L

L

D

E

C

I

D

S

L

A

P

A

V

E

I

G

L

V

Q

R

R
|
R

L

-

A

-

L

-

D

-

P

-

K

-

T

T

A

G

T

G

Q

V

R

R

P

P

S
x
L

L

L

T

A

G

G

R

P

H

D

V

R

Q

A

D

E

E

K

T

Y

S

R

Q

A

I

L

L

M

E

A

R

K

R
|
R

R

A

P

P

L

L

Y

Y

R

R

V

R

I

V

A

A

H

T

V

M

A

R

V

V

D

D

S

T

binding adenosine-5'-diphosphate: G11 (= G41), G13 (= G43), K14 (= K44), S15 (= S45), T16 (= T46), R109 (= R140)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D33 (= D63), F48 (≠ V78), R57 (= R87), G79 (= G109), G80 (= G110), L116 (≠ S153), R133 (= R170)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 150, 152

Query Sequence

>WP_084076347.1 NCBI__GCF_900129305.1:WP_084076347.1
MRETAGCLFYGARSLHFALLRRHWRVEESTIQRIALIGYRGTGKSTVGDLLAARLGWDFV
DMDRVLTQRFGTSIAHFVAQNGWEAFREAESALLGQISREEGLVVATGGGIVEKERNGKI
LRDCFHVVWLDCDLPVILQRLALDPKTATQRPSLTGRHVQDETSQILERRRPLYRVIAHV
AVDSGSLSAEEIAEVIARNLAKTGRAPRRPVEF

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory