SitesBLAST

O59791 Serine racemase; D-serine ammonia-lyase; D-serine dehydratase; L-serine ammonia-lyase; L-serine dehydratase; EC 5.1.1.18; EC 4.3.1.18; EC 4.3.1.17 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see 2 papers)
48% identity, 97% coverage: 5:292/296 of query aligns to 34:323/323 of O59791

query
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O59791

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K57 (= K28) active site, Proton acceptor; modified: Lysino-D-alanine (Lys); alternate; modified: N6-(pyridoxal phosphate)lysine; alternate
S82 (= S53) active site, Proton acceptor; mutation to A: Loss of racemase activity. Reduces D-serine dehydratase activity by 99%. Slightly reduced L-serine dehydratase activity.
N84 (= N55) binding pyridoxal 5'-phosphate
G183 (= G153) binding pyridoxal 5'-phosphate
G184 (= G154) binding pyridoxal 5'-phosphate
G185 (= G155) binding pyridoxal 5'-phosphate
G186 (= G156) binding pyridoxal 5'-phosphate
L187 (= L157) binding pyridoxal 5'-phosphate
E208 (= E178) binding Mg(2+)
G212 (≠ A182) binding Mg(2+)
D214 (= D184) binding Mg(2+)
S308 (= S277) binding pyridoxal 5'-phosphate

2zr8A Crystal structure of modified serine racemase complexed with serine (see paper)
48% identity, 97% coverage: 5:292/296 of query aligns to 30:319/319 of 2zr8A

query
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2zr8A

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active site: K53 (= K28), S78 (= S53), E204 (= E178), G208 (≠ A182), D210 (= D184), G232 (= G206), I303 (≠ L276), S304 (= S277)
binding magnesium ion: E204 (= E178), G208 (≠ A182), D210 (= D184)
binding n-(5'-phosphopyridoxyl)-d-alanine: F52 (= F27), K53 (= K28), S77 (= S52), S78 (= S53), N80 (= N55), H81 (= H56), P147 (= P121), G179 (= G153), G180 (= G154), G181 (= G155), G182 (= G156), G232 (= G206), E277 (= E251), T279 (≠ S253), S304 (= S277)
binding serine: S78 (= S53), R129 (= R104), D231 (= D205), G232 (= G206), A233 (≠ V207), Q234 (≠ R208), T235 (= T209)

2zpuA Crystal structure of modified serine racemase from s.Pombe. (see paper)
48% identity, 97% coverage: 5:292/296 of query aligns to 30:319/319 of 2zpuA

query
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2zpuA

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active site: K53 (= K28), S78 (= S53), E204 (= E178), G208 (≠ A182), D210 (= D184), G232 (= G206), I303 (≠ L276), S304 (= S277)
binding magnesium ion: E204 (= E178), G208 (≠ A182), D210 (= D184)
binding n-(5'-phosphopyridoxyl)-d-alanine: F52 (= F27), K53 (= K28), S77 (= S52), S78 (= S53), N80 (= N55), H81 (= H56), P147 (= P121), G179 (= G153), G180 (= G154), G181 (= G155), G182 (= G156), G232 (= G206), E277 (= E251), T279 (≠ S253), S304 (= S277)

Q9GZT4 Serine racemase; D-serine ammonia-lyase; D-serine dehydratase; L-serine ammonia-lyase; L-serine dehydratase; EC 5.1.1.18; EC 4.3.1.18; EC 4.3.1.17 from Homo sapiens (Human) (see 4 papers)
42% identity, 94% coverage: 6:282/296 of query aligns to 34:318/340 of Q9GZT4

query
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Q9GZT4

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K51 (≠ R23) binding ATP
T52 (≠ I24) binding ATP
K56 (= K28) modified: N6-(pyridoxal phosphate)lysine
P69 (= P41) binding Ca(2+)
T81 (= T50) binding Ca(2+)
N86 (= N55) binding pyridoxal 5'-phosphate
Q89 (= Q58) binding ATP
Y121 (= Y90) binding ATP
D178 (= D146) binding Mg(2+)
G185 (= G153) binding pyridoxal 5'-phosphate
G186 (= G154) binding pyridoxal 5'-phosphate
G187 (= G155) binding pyridoxal 5'-phosphate
G188 (= G156) binding pyridoxal 5'-phosphate
M189 (≠ L157) binding pyridoxal 5'-phosphate
E210 (= E178) binding Ca(2+); binding Mg(2+); binding Mn(2+)
A214 (= A182) binding Ca(2+); binding Mg(2+); binding Mn(2+)
D216 (= D184) binding Ca(2+); binding Mg(2+); binding Mn(2+)
N247 (≠ I215) binding Ca(2+); binding Mg(2+)
K279 (= K247) binding ATP
S313 (= S277) binding pyridoxal 5'-phosphate
N316 (= N280) binding ATP

Sites not aligning to the query:

13 binding Mg(2+)
31 binding ATP
32 binding ATP
33 binding ATP

7nbhAAA structure of human serine racemase in complex with DSiP fragment Z26781964, XChem fragment screen (see paper)
42% identity, 94% coverage: 6:282/296 of query aligns to 31:315/320 of 7nbhAAA

query
sites
7nbhAAA

L

L

D

N

K

Q

M

L

L

T

G

G

A

R

R

N

F

L

H

F

F

F

K

K

C

C

E

E

N

L

L

F

Q

Q

R

K

I

T

G

G

A

S

F

F

K
|
K

F

I

R

R

G

G

A

A

F

L

N

N

A

A

L

V

S

R

R

S

L

L

S

V

P

P

-

D

-

A

-

L

E

E

E

R

R

K

R

P

R

K

G

A

V

V

T

T

I

H

S

S

S
|
S

G

G

N

N

H

H

A
x
G

Q
|
Q

A

A

V

L

A

T

L

Y

V

A

G

A

R

K

E

L

L

E

G

G

I

I

D

P

T

A

T

Y

V

I

L

V

M

V

P

P

A

Q

N

T

A

A

P

P

E

D

I

C

K
|
K

R

K

R

L

A

A

V
x
I

E

Q

G

A

Y
|
Y

G

G

A

A

T

S

I

I

V

V

T

Y

Y

C

D

E

P

P

A

S

T

D

T

E

R

S

R

R

E

E

E

N

-

V

A

A

L

K

R

R

A

V

V

T

D

E

G

E

G

T

E

E

R

G

V

I

F

M

I

V

P

H

P

P

F

N

D

Q

H

E

W

P

D

A

V

V

I

I

A

A

G

G

Q

Q

G

G

T

T

A

I

G

A

L

L

E

E

L

V

A

L

Q

N

A

Q

V

V

S

P

H

L

L

V

D

D

M

A

L

L

L

V

V

V

P

P

C

V

G

G

L

M

L

L

S

A

G

G

C

I

A

A

V

I

A

T

V

V

K

K

G

A

Y

L

F

K

P

P

R

S

C

V

R

K

V

V

V

Y

G

A

V

A

E
|
E

P

P

E

S

S

N

A
|
A

D

D

D
|
D

A

C

T

Y

R

Q

S

S

F

K

R

L

M

K

G

G

T

K

L

L

Q

M

-

P

E

N

V

L

H

Y

N

P

P

P

P

E

T

T

I

I

A

A

D
|
D

G
|
G

V

V

R

K

T

S

P

-

R

S

L

I

G

G

D

L

I

N

T

T

F

W

P

P

L

I

V

I

L

R

A

D

H

L

V

V

D

D

M

I

R

F

T

T

V

V

S

T

E

E

E

D

A

E

I

I

R

K

E

C

A

A

V

T

R

Q

F

L

L

V

F

W

Y

E

R

R

M

M

K

K

L

L

V

L

V

I

E

E

P
|
P

S

T

G

A

A

G

L

V

G

G

L

V

A

A

L

V

L

L

S

S

-

Q

-

H

-

F

G

Q

A

T

V

V

K

S

P

P

-

E

T

V

G

K

D

N

V

I

G

C

I

I

L

V

L
|
L

S
|
S

G

G

N
|
N

V
|
V

D
|
D

active site: K53 (= K28), S81 (= S53), E207 (= E178), A211 (= A182), D213 (= D184), G236 (= G206), L309 (= L276), S310 (= S277)
binding calcium ion: E207 (= E178), A211 (= A182), D213 (= D184)
binding N-[(1H-benzimidazol-2-yl)methyl]furan-2-carboxamide: S81 (= S53), G85 (≠ A57), Q86 (= Q58), K111 (= K83), I115 (≠ V87), Y118 (= Y90), D235 (= D205), P281 (= P252), N313 (= N280), V314 (= V281), D315 (= D282)

7nbfAAA structure of human serine racemase in complex with DSiP fragment Z126932614, XChem fragment screen (see paper)
42% identity, 94% coverage: 6:282/296 of query aligns to 31:315/323 of 7nbfAAA

query
sites
7nbfAAA

L

L

D

N

K

Q

M

L

L

T

G

G

A

R

R

N

F

L

H

F

F

F

K

K

C

C

E

E

N

L

L
x
F

Q

Q

R

K

I

T

G

G

A

S

F
|
F

K
|
K

F

I

R

R

G

G

A

A

F

L

N

N

A

A

L

V

S

R

R

S

L

L

S

V

P

P

-

D

-

A

-

L

E

E

E

R

R

K

R

P

R

K

G

A

V

V

T

T

I

H

S

S

S
|
S

G

G

N
|
N

H

H

A

G

Q

Q

A

A

V

L

A

T

L

Y

V

A

G

A

R

K

E

L

L

E

G

G

I

I

D

P

T

A

T

Y

V

I

L

V

M

V

P

P

A

Q

N

T

A

A

P

P

E

D

I

C

K

K

R

K

R

L

A

A

V

I

E

Q

G

A

Y

Y

G

G

A

A

T

S

I

I

V

V

T

Y

Y

C

D

E

P

P

A

S

T

D

T

E

R

S

R

R

E

E

E

N

-

V

A

A

L

K

R

R

A

V

V

T

D

E

G

E

G

T

E

E

R

G

V

I

F

M

I

V

P

H

P

P

F

N

D

Q

H

E

W

P

D

A

V

V

I

I

A

A

G

G

Q

Q

G

G

T

T

A

I

G

A

L

L

E

E

L

V

A

L

Q

N

A

Q

V

V

S

P

H

L

L

V

D

D

M

A

L

L

L

V

V

V

P

P

C

V

G
|
G

L
x
M

L

L

S

A

G

G

C

I

A

A

V

I

A

T

V

V

K

K

G

A

Y

L

F

K

P

P

R

S

C

V

R

K

V

V

V

Y

G

A

V

A

E
|
E

P

P

E

S

S

N

A
|
A

D

D

D
|
D

A

C

T

Y

R

Q

S

S

F

K

R

L

M

K

G

G

T

K

L

L

Q

M

-

P

E

N

V

L

H

Y

N

P

P

P

P

E

T

T

I

I

A

A

D

D

G
|
G

V
|
V

R

K

T

S

P

-

R

S

L

I

G

G

D

L

I
x
N

T

T

F

W

P

P

L

I

V

I

L

R

A

D

H

L

V

V

D

D

M

I

R

F

T

T

V

V

S

T

E

E

E

D

A

E

I

I

R

K

E

C

A

A

V

T

R

Q

F

L

L

V

F

W

Y

E

R

R

M

M

K

K

L

L

V

L

V

I

E

E

P

P

S
x
T

G

A

A

G

L

V

G

G

L

V

A

A

L

V

L

L

S

S

-

Q

-

H

-

F

G

Q

A

T

V

V

K

S

P

P

-

E

T

V

G

K

D

N

V

I

G

C

I

I

L

V

L
|
L

S
|
S

G
|
G

G

G

N

N

V

V

D

D

active site: K53 (= K28), S81 (= S53), E207 (= E178), A211 (= A182), D213 (= D184), G236 (= G206), L309 (= L276), S310 (= S277)
binding calcium ion: E207 (= E178), A211 (= A182), D213 (= D184)
binding magnesium ion: N244 (≠ I215)
binding pyridoxal-5'-phosphate: F52 (= F27), K53 (= K28), N83 (= N55), G182 (= G153), G183 (= G154), G184 (= G155), G185 (= G156), M186 (≠ L157), G236 (= G206), V237 (= V207), T282 (≠ S253), S310 (= S277), G311 (= G278)
binding 2-[(methylsulfonyl)methyl]-1H-benzimidazole: F46 (≠ L21)

Sites not aligning to the query:

7nbdAAA structure of human serine racemase in complex with DSiP fragment Z235449082, XChem fragment screen (see paper)
42% identity, 94% coverage: 6:282/296 of query aligns to 31:315/323 of 7nbdAAA

query
sites
7nbdAAA

L

L

D

N

K

Q

M

L

L

T

G

G

A

R

R

N

F

L

H

F

F

F

K

K

C

C

E

E

N

L

L

F

Q

Q

R

K

I

T

G

G

A

S

F
|
F

K
|
K

F

I

R

R

G

G

A

A

F

L

N

N

A

A

L

V

S

R

R

S

L

L

S

V

P

P

-

D

-

A

-

L

E

E

E

R

R

K

R

P

R

K

G

A

V

V

T

T

I

H

S

S

S
|
S

G

G

N
|
N

H

H

A

G

Q

Q

A

A

V

L

A

T

L

Y

V

A

G

A

R

K

E

L

L

E

G

G

I

I

D

P

T

A

T

Y

V

I

L

V

M

V

P

P

A

Q

N

T

A

A

P

P

E

D

I

C

K

K

R

K

R

L

A

A

V

I

E

Q

G

A

Y

Y

G

G

A

A

T

S

I

I

V

V

T

Y

Y

C

D

E

P

P

A

S

T

D

T

E

R

S

R

R

E

E

E

N

-

V

A

A

L

K

R

R

A

V

V

T

D

E

G

E

G

T

E

E

R

G

V

I

F

M

I

V

P

H

P

P

F

N

D

Q

H

E

W

P

D

A

V

V

I

I

A

A

G

G

Q

Q

G

G

T

T

A

I

G

A

L

L

E

E

L

V

A

L

Q

N

A

Q

V

V

S

P

H

L

L

V

D

D

M

A

L

L

L

V

V

V

P

P

C

V

G
|
G

L
x
M

L

L

S

A

G

G

C

I

A

A

V

I

A

T

V

V

K

K

G

A

Y

L

F

K

P

P

R

S

C

V

R

K

V

V

V

Y

G

A

V

A

E
|
E

P

P

E

S

S

N

A
|
A

D

D

D
|
D

A

C

T

Y

R

Q

S

S

F

K

R

L

M

K

G

G

T

K

L

L

Q

M

-

P

E

N

V

L

H

Y

N

P

P

P

P

E

T

T

I

I

A

A

D

D

G
|
G

V
|
V

R

K

T

S

P

-

R

S

L

I

G

G

D

L

I
x
N

T

T

F

W

P

P

L

I

V

I

L

R

A

D

H

L

V

V

D

D

M

I

R

F

T

T

V

V

S

T

E

E

E

D

A

E

I

I

R

K

E

C

A

A

V

T

R

Q

F

L

L

V

F
x
W

Y

E

R

R

M

M

K

K

L

L

V
x
L

V

I

E
|
E

P

P

S
x
T

G

A

A

G

L

V

G

G

L

V

A

A

L

V

L

L

S

S

-

Q

-

H

-

F

G

Q

A

T

V

V

K

S

P

P

-

E

T

V

G

K

D

N

V

I

G

C

I

I

L

V

L
|
L

S
|
S

G
|
G

G

G

N

N

V
|
V

D

D

active site: K53 (= K28), S81 (= S53), E207 (= E178), A211 (= A182), D213 (= D184), G236 (= G206), L309 (= L276), S310 (= S277)
binding calcium ion: E207 (= E178), A211 (= A182), D213 (= D184)
binding [4-(1H-benzimidazol-1-yl)phenyl]methanol: W272 (≠ F243), L278 (≠ V249), V314 (= V281)
binding magnesium ion: N244 (≠ I215)
binding pyridoxal-5'-phosphate: F52 (= F27), K53 (= K28), N83 (= N55), G182 (= G153), G183 (= G154), G184 (= G155), G185 (= G156), M186 (≠ L157), G236 (= G206), V237 (= V207), E280 (= E251), T282 (≠ S253), S310 (= S277), G311 (= G278)

Sites not aligning to the query:

7nbcCCC structure of human serine racemase in complex with DSiP fragment Z2856434779, XChem fragment screen (see paper)
42% identity, 94% coverage: 6:282/296 of query aligns to 31:315/323 of 7nbcCCC

query
sites
7nbcCCC

L

L

D

N

K

Q

M

L

L

T

G

G

A

R

R

N

F

L

H

F

F

F

K

K

C

C

E

E

N

L

L

F

Q

Q

R

K

I

T

G

G

A

S

F
|
F

K
|
K

F

I

R

R

G

G

A

A

F

L

N

N

A

A

L

V

S

R

R

S

L

L

S

V

P

P

-

D

-

A

-

L

E

E

E

R

R

K

R

P

R

K

G

A

V

V

T
|
T

I
x
H

S

S

S
|
S

G

G

N
|
N

H
|
H

A

G

Q

Q

A

A

V

L

A

T

L

Y

V

A

G

A

R

K

E

L

L

E

G

G

I

I

D

P

T

A

T

Y

V

I

L

V

M

V

P

P

A

Q

N

T

A

A

P

P

E

D

I

C

K

K

R

K

R

L

A

A

V

I

E

Q

G

A

Y

Y

G

G

A

A

T

S

I

I

V

V

T

Y

Y

C

D

E

P

P

A

S

T

D

T

E

R

S

R

R

E

E

E

N

-

V

A

A

L

K

R

R

A

V

V

T

D

E

G

E

G

T

E

E

R

G

V

I

F

M

I
x
V

P
x
H

P
|
P

F

N

D

Q

H

E

W

P

D

A

V

V

I

I

A

A

G

G

Q

Q

G

G

T

T

A

I

G

A

L

L

E

E

L

V

A

L

Q

N

A

Q

V

V

S

P

H

L

L

V

D

D

M

A

L

L

L

V

V

V

P

P

C

V

G
|
G

L
x
M

L

L

S

A

G

G

C

I

A

A

V

I

A

T

V

V

K

K

G

A

Y

L

F

K

P

P

R

S

C

V

R

K

V

V

V

Y

G

A

V

A

E
|
E

P

P

E

S

S

N

A
|
A

D

D

D
|
D

A

C

T

Y

R

Q

S

S

F

K

R

L

M

K

G

G

T

K

L

L

Q

M

-

P

E

N

V

L

H

Y

N

P

P

P

P

E

T

T

I

I

A

A

D

D

G
|
G

V
|
V

R

K

T

S

P

-

R

S

L

I

G

G

D

L

I

N

T

T

F

W

P

P

L

I

V

I

L

R

A

D

H

L

V

V

D

D

M

I

R

F

T

T

V

V

S

T

E

E

E

D

A

E

I

I

R

K

E

C

A

A

V

T

R

Q

F

L

L

V

F

W

Y

E

R

R

M

M

K

K

L

L

V

L

V

I

E

E

P

P

S
x
T

G

A

A

G

L

V

G

G

L

V

A

A

L

V

L

L

S

S

-

Q

-

H

-

F

G

Q

A

T

V

V

K

S

P

P

-

E

T

V

G

K

D

N

V

I

G

C

I

I

L

V

L
|
L

S
|
S

G
|
G

G

G

N

N

V

V

D

D

active site: K53 (= K28), S81 (= S53), E207 (= E178), A211 (= A182), D213 (= D184), G236 (= G206), L309 (= L276), S310 (= S277)
binding biphenyl-4-ylacetic acid: T78 (= T50), H79 (≠ I51), H84 (= H56), V148 (≠ I119), H149 (≠ P120), P150 (= P121)
binding calcium ion: E207 (= E178), A211 (= A182), D213 (= D184)
binding pyridoxal-5'-phosphate: F52 (= F27), K53 (= K28), N83 (= N55), G182 (= G153), G183 (= G154), G184 (= G155), G185 (= G156), M186 (≠ L157), G236 (= G206), V237 (= V207), T282 (≠ S253), S310 (= S277), G311 (= G278)

7nbcAAA structure of human serine racemase in complex with DSiP fragment Z2856434779, XChem fragment screen (see paper)
42% identity, 94% coverage: 6:282/296 of query aligns to 31:315/323 of 7nbcAAA

query
sites
7nbcAAA

L

L

D

N

K

Q

M

L

L

T

G

G

A

R

R

N

F

L

H

F

F

F

K

K

C

C

E

E

N

L

L

F

Q

Q

R

K

I

T

G

G

A

S

F
|
F

K
|
K

F

I

R

R

G

G

A

A

F

L

N

N

A

A

L

V

S

R

R

S

L

L

S

V

P

P

-

D

-

A

-

L

E

E

E

R

R

K

R

P

R

K

G

A

V

V

T

T

I

H

S

S

S
|
S

G

G

N
|
N

H

H

A

G

Q

Q

A

A

V

L

A

T

L

Y

V

A

G

A

R

K

E

L

L

E

G

G

I

I

D

P

T

A

T

Y

V

I

L

V

M

V

P

P

A

Q

N

T

A

A

P

P

E

D

I

C

K

K

R

K

R

L

A

A

V

I

E

Q

G

A

Y

Y

G

G

A

A

T

S

I

I

V

V

T

Y

Y

C

D

E

P

P

A

S

T

D

T

E

R

S

R

R

E

E

E

N

-

V

A

A

L

K

R

R

A

V

V

T

D

E

G

E

G

T

E

E

R

G

V

I

F

M

I

V

P

H

P

P

F

N

D

Q

H

E

W

P

D

A

V

V

I

I

A

A

G

G

Q

Q

G

G

T

T

A

I

G

A

L

L

E

E

L

V

A

L

Q

N

A

Q

V

V

S

P

H

L

L

V

D

D

M

A

L

L

L

V

V

V

P

P

C

V

G
|
G

L
x
M

L

L

S

A

G

G

C

I

A

A

V

I

A

T

V

V

K

K

G

A

Y

L

F

K

P

P

R

S

C

V

R

K

V

V

V

Y

G

A

V

A

E
|
E

P

P

E

S

S

N

A
|
A

D

D

D
|
D

A

C

T

Y

R

Q

S

S

F

K

R

L

M

K

G

G

T

K

L

L

Q

M

-

P

E

N

V

L

H

Y

N

P

P

P

P

E

T

T

I

I

A

A

D

D

G
|
G

V
|
V

R

K

T

S

P

-

R

S

L

I

G

G

D

L

I
x
N

T

T

F

W

P

P

L

I

V

I

L

R

A

D

H

L

V

V

D

D

M

I

R

F

T

T

V

V

S

T

E

E

E

D

A

E

I

I

R

K

E

C

A

A

V

T

R

Q

F

L

L

V

F

W

Y

E

R

R

M

M

K

K

L

L

V

L

V

I

E

E

P

P

S
x
T

G

A

A

G

L

V

G

G

L

V

A

A

L

V

L

L

S

S

-

Q

-

H

-

F

G

Q

A

T

V

V

K

S

P

P

-

E

T

V

G

K

D

N

V

I

G

C

I

I

L

V

L
|
L

S
|
S

G
|
G

G

G

N

N

V

V

D

D

active site: K53 (= K28), S81 (= S53), E207 (= E178), A211 (= A182), D213 (= D184), G236 (= G206), L309 (= L276), S310 (= S277)
binding calcium ion: E207 (= E178), A211 (= A182), D213 (= D184)
binding magnesium ion: N244 (≠ I215)
binding pyridoxal-5'-phosphate: F52 (= F27), K53 (= K28), N83 (= N55), G182 (= G153), G183 (= G154), G184 (= G155), G185 (= G156), M186 (≠ L157), G236 (= G206), V237 (= V207), T282 (≠ S253), S310 (= S277), G311 (= G278)

Sites not aligning to the query:

binding magnesium ion: 3

7nbgAAA structure of human serine racemase in complex with DSiP fragment Z52314092, XChem fragment screen (see paper)
42% identity, 94% coverage: 6:282/296 of query aligns to 31:315/322 of 7nbgAAA

query
sites
7nbgAAA

L

L

D

N

K

Q

M

L

L

T

G

G

A

R

R

N

F

L

H

F

F

F

K

K

C

C

E

E

N

L

L

F

Q

Q

R

K

I

T

G

G

A

S

F
|
F

K
|
K

F

I

R

R

G

G

A

A

F

L

N

N

A

A

L

V

S

R

R

S

L

L

S

V

P

P

-

D

-

A

-

L

E

E

E

R

R

K

R

P

R

K

G

A

V

V

T

T

I

H

S

S

S
|
S

G

G

N
|
N

H

H

A
x
G

Q
|
Q

A

A

V

L

A

T

L

Y

V

A

G

A

R

K

E

L

L

E

G

G

I

I

D

P

T

A

T

Y

V
x
I

L

V

M

V

P

P

A

Q

N

T

A

A

P

P

E

D

I

C

K
|
K

R

K

R

L

A

A

V
x
I

E

Q

G

A

Y
|
Y

G

G

A

A

T

S

I

I

V

V

T

Y

Y

C

D

E

P

P

A

S

T

D

T

E

R

S

R

R

E

E

E

N

-

V

A

A

L

K

R

R

A

V

V

T

D

E

G

E

G

T

E

E

R

G

V

I

F

M

I

V

P

H

P

P

F

N

D

Q

H

E

W

P

D

A

V

V

I

I

A

A

G

G

Q

Q

G

G

T

T

A

I

G

A

L

L

E

E

L

V

A

L

Q

N

A

Q

V

V

S

P

H

L

L

V

D

D

M

A

L

L

L

V

V

V

P

P

C

V

G
|
G

L
x
M

L

L

S

A

G

G

C

I

A

A

V

I

A

T

V

V

K

K

G

A

Y

L

F

K

P

P

R

S

C

V

R

K

V

V

V

Y

G

A

V

A

E
|
E

P

P

E

S

S

N

A
|
A

D

D

D
|
D

A

C

T

Y

R

Q

S

S

F

K

R

L

M

K

G

G

T

K

L

L

Q

M

-

P

E

N

V

L

H

Y

N

P

P

P

P

E

T

T

I

I

A

A

D

D

G
|
G

V
|
V

R

K

T

S

P

-

R

S

L

I

G

G

D

L

I

N

T

T

F

W

P

P

L

I

V

I

L

R

A

D

H

L

V

V

D

D

M

I

R

F

T

T

V

V

S

T

E

E

E

D

A

E

I

I

R

K

E

C

A

A

V

T

R

Q

F

L

L

V

F

W

Y

E

R

R

M

M

K

K

L

L

V

L

V

I

E

E

P

P

S
x
T

G

A

A

G

L

V

G

G

L

V

A

A

L

V

L

L

S

S

-

Q

-

H

-

F

G

Q

A

T

V

V

K

S

P

P

-

E

T

V

G

K

D

N

V

I

G

C

I

I

L

V

L
|
L

S
|
S

G
|
G

G

G

N

N

V

V

D

D

active site: K53 (= K28), S81 (= S53), E207 (= E178), A211 (= A182), D213 (= D184), G236 (= G206), L309 (= L276), S310 (= S277)
binding calcium ion: E207 (= E178), A211 (= A182), D213 (= D184)
binding pyridoxal-5'-phosphate: F52 (= F27), K53 (= K28), N83 (= N55), G182 (= G153), G183 (= G154), G184 (= G155), G185 (= G156), M186 (≠ L157), G236 (= G206), V237 (= V207), T282 (≠ S253), S310 (= S277), G311 (= G278)
binding ~{N}-[2-(2-methylphenyl)ethyl]ethanamide: S81 (= S53), G85 (≠ A57), Q86 (= Q58), I101 (≠ V73), K111 (= K83), I115 (≠ V87), Y118 (= Y90)

Q7XSN8 Serine racemase; D-serine dehydratase; D-serine ammonia-lyase; L-serine dehydratase; L-serine ammonia-lyase; EC 5.1.1.18; EC 4.3.1.18; EC 4.3.1.17 from Oryza sativa subsp. japonica (Rice) (see paper)
41% identity, 99% coverage: 1:293/296 of query aligns to 41:335/339 of Q7XSN8

query
sites
Q7XSN8

M

L

G

S

C

S

R

T

T

S

L

I

D

D

K

A

M

I

L

V

G

G

A

K

R

Q

F

L

H

F

F

F

K

K

C

C

E

E

N

C

L

F

Q

Q

R

K

I

A

G

G

A

A

F

F

K

K

F

I

R

R

G

G

A

A

F

S

N

N

A

S

L

I

S

F

R

A

L

L

S

D

P

D

E

D

E

E

R

A

R

S

R

K

G

G

V

V

T

T

I

H

S

S

G

G

N

N

H

H

A

A

Q

A

A

A

V

V

A

A

L

L

V

A

G

A

R

K

E

L

L

R

G

G

I

I

D

P

T

A

T

Y

V

I

L

V

M

I

P

P

A

R

N

N

A

A

P

P

E

A

I

C

K

K

R

V

R

D

A

N

V

V

E

K

G

R

Y

Y

G

G

A

G

T

H

I

I

V

I

T

W

Y

S

D

D

P

V

A

S

T

I

T

E

R

S

R

R

E

E

E

S

-

V

A

A

L

K

R

R

A

V

V

Q

D

E

G

E

G

T

E

G

R

A

V

I

F

L

I

V

P

H

P

P

F

F

D

N

H

N

W

K

D

N

V

T

I

I

A

S

G

G

Q

Q

G

G

T

T

A

V

G

S

L

L

E

E

L

L

A

L

Q

E

A

E

V

V

S

P

H

E

L

I

D

D

M

T

L

I

V

V

P

P

C

I

G

S

G

G

L

L

L

I

S

S

G

G

C

V

A

A

V

L

A

A

V

A

K

K

G

A

Y

I

F

N

P

P

R

S

C

I

R

R

V

I

V

L

G

A

V

A

E
|
E

P

P

E

K

S

G

A

A

D

D

D
|
D

A

S

T

A

R

Q

S

S

F

K

R

A

M

A

G

G

T

K

L

I

Q

I

E

T

V

L

H

P

N

S

P

T

P

N

T

T

I

I

A

A

D

D

G

G

V

L

R

R

T

A

P

-

R

F

L

L

G

G

D

D

I

L

T

T

F

W

P

P

L

V

V

V

L

R

A

D

H

L

V

V

D

D

M

I

R

I

T

V

V

V

S

D

E

D

E

N

A

A

I

I

R

V

E

D

A

A

V

M

R

K

F

M

L

C

F

Y

Y

E

R

M

M

L

K

K

L

V

V

A

V

V

E

E

P

P

S

S

G

G

A

A

L

I

G

G

L

L

A

A

L

A

L

L

S

S

G

D

A

E

V

F

K

K

-

Q

-

S

-

A

-

W

-

H

P

E

T

S

G

S

D

K

V

I

G

G

I

I

L

I

L

V

S

S

G

G

N

N

V

V

D

D

G

-

Q

-

T

-

M

-

A

L

G

G

I

V

L

L

S

W

E

E

S

S

E219 (= E178) mutation to A: Reduces catalytic activity and abolishes the regulatory effect of Mg(2+) addition; when associated with A-225.
D225 (= D184) mutation to A: Reduces catalytic activity and abolishes the regulatory effect of Mg(2+) addition; when associated with A-219.

6zspAAA serine racemase bound to atp and malonate. (see paper)
42% identity, 94% coverage: 6:282/296 of query aligns to 31:308/320 of 6zspAAA

query
sites
6zspAAA

L

L

D

N

K

Q

M

L

L

T

G

G

A

R

R

N

F

L

H

F

F

F

K

K

C

C

E

E

N

L

L

F

Q

Q

R
x
K

I
x
T

G

G

A

S

F

F

K
|
K

F

I

R

R

G

G

A

A

F

L

N

N

A

A

L

V

S

R

R

S

L

L

S

K

P

P

E

-

R

-

R

K

G

A

V

V

T

T

I

H

S
|
S

G

G

N
|
N

H
|
H

A

G

Q
|
Q

A

A

V

L

A

T

L

Y

V

A

G

A

R

K

E

L

L

E

G

G

I

I

D

P

T

A

T

Y

V

I

L

V

M

V

P

P

A

Q

N

T

A

A

P

P

E

D

I

C

K

K

R

K

R

L

A

A

V

I

E

Q

G

A

Y
|
Y

G

G

A

A

T

S

I

I

V

V

T

Y

Y

C

D

E

P

P

A

S

T

D

T

E

R

S

R
|
R

E

E

E

N

-

V

A

A

L

K

R

R

A

V

V

T

D

E

G

E

G

T

E

E

R

G

V

I

F

M

I

V

P

H

P

P

F

N

D

Q

H

E

W

P

D

A

V

V

I

I

A

A

G

G

Q

Q

G

G

T

T

A

I

G

A

L

L

E

E

L

V

A

L

Q

N

A

Q

V

V

S

P

H

L

L

V

D

D

M

A

L

L

L

V

V

V

P

P

C

V

G

G

L

M

L

L

S

A

G

G

C

I

A

A

V

I

A

T

V

V

K

K

G

A

Y

L

F

K

P

P

R

S

C

V

R

K

V

V

V

Y

G

A

V

A

E
|
E

P

P

E

S

S

N

A
|
A

D

D

D
|
D

A

C

T

Y

R

Q

S

S

F

K

R

L

M

K

G

G

T

K

L

L

Q

M

-

P

E

N

V

L

H

Y

N

P

P

P

P

E

T

T

I

I

A

A

D

D

G
|
G

V

V

R

K

T
x
S

P

-

R

S

L

I

G

G

D

L

I

N

T

T

F

W

P

P

L

I

V

I

L

R

A

D

H

L

V

V

D

D

M

I

R

F

T

T

V

V

S

T

E

E

E

D

A

E

I

I

R

K

E

C

A

A

V

T

R

Q

F

L

L

V

F

W

Y
x
E

R
|
R

M

M

K
|
K

L

L

V

L

V

I

E

E

P

P

S

T

G

A

A

G

L

V

G

G

L

V

A

A

L

V

L

L

S

S

-

Q

-

H

-

F

G

Q

A

T

V

V

K

S

P

P

-

E

T

V

G

K

D

N

V

I

G

C

I

I

L

V

L
|
L

S
|
S

G

G

N
|
N

V

V

D

D

active site: K53 (= K28), S74 (= S53), E200 (= E178), A204 (= A182), D206 (= D184), G229 (= G206), L302 (= L276), S303 (= S277)
binding adenosine-5'-triphosphate: K48 (≠ R23), T49 (≠ I24), Q79 (= Q58), Y111 (= Y90), E266 (≠ Y244), R267 (= R245), K269 (= K247), N306 (= N280)
binding magnesium ion: E200 (= E178), A204 (= A182), D206 (= D184)
binding malonate ion: K53 (= K28), S73 (= S52), S74 (= S53), N76 (= N55), H77 (= H56), R125 (= R104), G229 (= G206), S232 (≠ T209)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 28, 29, 30

3l6bA X-ray crystal structure of human serine racemase in complex with malonate a potent inhibitor (see paper)
41% identity, 94% coverage: 6:282/296 of query aligns to 32:311/322 of 3l6bA

query
sites
3l6bA

L

L

D

N

K

Q

M

L

L

T

G

G

A

R

R

N

F

L

H

F

F

F

K

K

C

C

E

E

N

L

L

F

Q

Q

R

K

I

T

G

G

A

S

F
|
F

K
|
K

F

I

R

R

G

G

A

A

F

L

N

N

A

A

L

V

S

R

R

S

L

L

S

V

P

-

E

-

E

R

R

K

R

P

R

K

G

A

V

V

T

T

I

H

S
|
S

G

G

N
|
N

H
|
H

A

G

Q

Q

A

A

V

L

A

T

L

Y

V

A

G

A

R

K

E

L

L

E

G

G

I

I

D

P

T

A

T

Y

V

I

L

V

M

V

P

P

A

Q

N

T

A

A

P

P

E

D

I

C

K

K

R

K

R

L

A

A

V

I

E

Q

G

A

Y

Y

G

G

A

A

T

S

I

I

V

V

T

Y

Y

C

D

E

P

P

A

S

T

D

T

E

R

S

R
|
R

E

E

E

N

-

V

A

A

L

K

R

R

A

V

V

T

D

E

G

E

G

T

E

E

R

G

V

I

F

M

I

V

P

H

P

P

F

N

D

Q

H

E

W

P

D

A

V

V

I

I

A

A

G

G

Q

Q

G

G

T

T

A

I

G

A

L

L

E

E

L

V

A

L

Q

N

A

Q

V

V

S

P

H

L

L

V

D

D

M

A

L

L

L

V

V

V

P

P

C

V

G
|
G

L
x
M

L

L

S

A

G

G

C

I

A

A

V

I

A

T

V

V

K

K

G

A

Y

L

F

K

P

P

R

S

C

V

R

K

V

V

V

Y

G

A

V

A

E
|
E

P

P

E

S

S

N

A
|
A

D

D

D
|
D

A

C

T

Y

R

Q

S

S

F

K

R

L

M

K

G

G

T

K

L

L

Q

M

-

P

E

N

V

L

H

Y

N

P

P

P

P

E

T

T

I

I

A

A

D

D

G
|
G

V
|
V

R

K

T

S

P

-

R

S

L

I

G

G

D

L

I

N

T

T

F

W

P

P

L

I

V

I

L

R

A

D

H

L

V

V

D

D

M

I

R

F

T

T

V

V

S

T

E

E

E

D

A

E

I

I

R

K

E

C

A

A

V

T

R

Q

F

L

L

V

F

W

Y

E

R

R

M

M

K

K

L

L

V

L

V

I

E
|
E

P

P

S
x
T

G

A

A

G

L

V

G

G

L

V

A

A

L

V

L

L

S

S

-

Q

-

H

-

F

G

Q

A

T

V

V

K

S

P

P

-

E

T

V

G

K

D

N

V

I

G

C

I

I

L

V

L
|
L

S
|
S

G
|
G

G

G

N

N

V

V

D

D

active site: K54 (= K28), S77 (= S53), E203 (= E178), A207 (= A182), D209 (= D184), G232 (= G206), T278 (≠ S253), L305 (= L276), S306 (= S277)
binding malonate ion: K54 (= K28), S76 (= S52), S77 (= S53), N79 (= N55), H80 (= H56), R128 (= R104), G232 (= G206)
binding manganese (ii) ion: E203 (= E178), A207 (= A182), D209 (= D184)
binding pyridoxal-5'-phosphate: F53 (= F27), K54 (= K28), N79 (= N55), G178 (= G153), G179 (= G154), G180 (= G155), G181 (= G156), M182 (≠ L157), V233 (= V207), E276 (= E251), T278 (≠ S253), S306 (= S277), G307 (= G278)

7nbgDDD structure of human serine racemase in complex with DSiP fragment Z52314092, XChem fragment screen (see paper)
41% identity, 94% coverage: 6:282/296 of query aligns to 31:310/310 of 7nbgDDD

query
sites
7nbgDDD

L

L

D

N

K

Q

M

L

L

T

G

G

A

R

R

N

F

L

H

F

F

F

K

K

C

C

E

E

N

L

L

F

Q

Q

R

K

I

T

G

G

A

S

F
|
F

K
|
K

F

I

R

R

G

G

A

A

F

L

N

N

A

A

L

V

S

R

R

S

L

L

S

V

P

-

E

-

E

R

R

K

R

P

R

K

G

A

V

V

T

T

I

H

S

S

S
|
S

G

G

N
|
N

H

H

A

G

Q
|
Q

A

A

V

L

A

T

L

Y

V

A

G

A

R

K

E

L

L

E

G

G

I

I

D

P

T

A

T

Y

V
x
I

L

V

M

V

P

P

A

Q

N

T

A

A

P

P

E

D

I

C

K

K

R

K

R

L

A

A

V

I

E

Q

G

A

Y
|
Y

G

G

A

A

T

S

I

I

V

V

T

Y

Y

C

D

E

P

P

A

S

T

D

T

E

R

S

R

R

E

E

E

N

-

V

A

A

L

K

R

R

A

V

V

T

D

E

G

E

G

T

E

E

R

G

V

I

F

M

I

V

P

H

P

P

F

N

D

Q

H

E

W

P

D

A

V

V

I

I

A

A

G

G

Q

Q

G

G

T

T

A

I

G

A

L

L

E

E

L

V

A

L

Q

N

A

Q

V

V

S

P

H

L

L

V

D

D

M

A

L

L

L

V

V

V

P

P

C

V

G
|
G

L
x
M

L

L

S

A

G

G

C

I

A

A

V

I

A

T

V

V

K

K

G

A

Y

L

F

K

P

P

R

S

C

V

R

K

V

V

V

Y

G

A

V

A

E
|
E

P

P

E

S

S

N

A
|
A

D

D

D
|
D

A

C

T

Y

R

Q

S

S

F

K

R

L

M

K

G

G

T

K

L

L

Q

M

-

P

E

N

V

L

H

Y

N

P

P

P

P

E

T

T

I

I

A

A

D

D

G
|
G

V
|
V

R

K

T

S

P

-

R

S

L

I

G

G

D

L

I
x
N

T

T

F

W

P

P

L

I

V

I

L

R

A

D

H

L

V

V

D

D

M

I

R

F

T

T

V

V

S

T

E

E

E

D

A

E

I

I

R

K

E

C

A

A

V

T

R

Q

F

L

L

V

F

W

Y

E

R

R

M

M

K

K

L

L

V

L

V

I

E
|
E

P

P

S
x
T

G

A

A

G

L

V

G

G

L

V

A

A

L

V

L

L

S

S

-

Q

-

H

-

F

G

Q

A

T

V

V

K

S

P

P

-

E

T

V

G

K

D

N

V

I

G

C

I

I

L

V

L
|
L

S
|
S

G
|
G

G

G

N

N

V

V

D

D

active site: K53 (= K28), S76 (= S53), E202 (= E178), A206 (= A182), D208 (= D184), G231 (= G206), L304 (= L276), S305 (= S277)
binding calcium ion: E202 (= E178), A206 (= A182), D208 (= D184)
binding magnesium ion: N239 (≠ I215)
binding ortho-xylene: S76 (= S53), Q81 (= Q58), I96 (≠ V73), Y113 (= Y90)
binding pyridoxal-5'-phosphate: F52 (= F27), K53 (= K28), N78 (= N55), G177 (= G153), G178 (= G154), G179 (= G155), G180 (= G156), M181 (≠ L157), G231 (= G206), V232 (= V207), E275 (= E251), T277 (≠ S253), S305 (= S277), G306 (= G278)

Sites not aligning to the query:

binding magnesium ion: 3

5cvcA Structure of maize serine racemase (see paper)
42% identity, 97% coverage: 1:286/296 of query aligns to 25:316/329 of 5cvcA

query
sites
5cvcA

M

M

G

S

C

S

R

T

T

S

L

I

D

D

K

A

M

M

L

V

G

G

A

K

R

K

F

L

H

F

F

F

K

K

C

C

E

E

N

C

L

F

Q

Q

R

K

I

A

G

G

A

A

F
|
F

K
|
K

F

I

R

R

G

G

A

A

F

S

N

N

A

S

L

I

S

F

R

A

L

L

S

D

P

D

E

E

E

Q

R

V

R

S

R

K

G

G

V

V

T

T

I

H

S

S

S
|
S

G

G

N
|
N

H

H

A

A

Q

A

A

A

V

V

A

A

L

L

V

A

G

A

R

K

E

L

L

R

G

G

I

I

D

P

T

A

T

H

V

I

L

V

M

I

P

P

A

R

N

N

A

A

P

P

E

A

I

S

K

K

R

V

R

E

A

N

V

V

E

K

G

C

Y

Y

G

G

A

G

T

H

I

I

V

I

T

W

Y

S

D

D

P

A

A

S

T

I

T

E

R

S

R

R

E

E

E

Y

-

V

A

S

L

K

R

R

A

V

V

Q

D

E

G

E

G

T

E

G

R

A

V

V

F

L

I

I

P

H

P

P

F

I

D

N

H

S

W

K

D

Y

V

T

I

I

A

S

G

G

Q

Q

G

G

T

T

A

V

G

S

L

L

E

E

L

L

A

L

Q

E

A

Q

V

V

S

P

H

E

L

I

D

D

M

T

L

I

V

V

P

P

C

I

G
x
S

G
|
G

L

L

L

I

S

S

G

G

C

V

A

A

V

L

A

A

V

A

K

K

G

A

Y

I

F

N

P

P

R

S

C

I

R

R

V

I

V

L

G

A

V

A

E
|
E

P

P

E

K

S

G

A
|
A

D

D

D
|
D

A

S

T

A

R

Q

S

S

F

K

R

A

M

A

G

G

T

K

L

I

Q

I

E

T

V

L

H

P

N

S

P

T

P

N

T

T

I

I

A

A

D

D

G
|
G

V
x
L

R

R

T

A

P

-

R

F

L

L

G

G

D

D

I

L

T

T

F

W

P

P

L

V

V

V

L

R

A

D

H

L

V

V

D

D

M

V

R

I

T

V

V

V

S

D

E

D

E

T

A

A

I

I

R

V

E

D

A

A

V

M

R

K

F

M

L

C

F

Y

Y

E

R

I

M

L

K

K

L

V

V

A

V

V

E
|
E

P

P

S

S

G

G

A

A

L

I

G

G

L

L

A

A

L

A

L

L

S

S

G

D

A

E

V

F

K

K

P

Q

-

S

-

A

-

W

-

H

-

E

T

S

G

S

D

K

V

I

G

G

I

I

L

I

L
x
V

S
|
S

G
|
G

G

G

N

N

V

V

D

D

G

L

Q

G

T

T

M

L

active site: K52 (= K28), S77 (= S53), E203 (= E178), A207 (= A182), D209 (= D184), G231 (= G206), V306 (≠ L276), S307 (= S277)
binding magnesium ion: E203 (= E178), A207 (= A182), D209 (= D184)
binding pyridoxal-5'-phosphate: F51 (= F27), K52 (= K28), N79 (= N55), S178 (≠ G153), G179 (= G154), G180 (= G155), G181 (= G156), L232 (≠ V207), E275 (= E251), S307 (= S277), G308 (= G278)

Q9QZX7 Serine racemase; D-serine ammonia-lyase; D-serine dehydratase; L-serine ammonia-lyase; L-serine dehydratase; EC 5.1.1.18; EC 4.3.1.18; EC 4.3.1.17 from Mus musculus (Mouse) (see paper)
42% identity, 94% coverage: 6:282/296 of query aligns to 34:318/339 of Q9QZX7

query
sites
Q9QZX7

L

L

D

N

K

Q

M

I

L

A

G

G

A

R

R

N

F

L

H

F

F

F

K

K

C

C

E

E

N

L

L

F

Q

Q

R

K

I

T

G

G

A

S

F

F

K

K

F

I

R

R

G

G

A

A

F

L

N

N

A

A

L

I

S

R

R

G

L

L

-

I

-

P

-

D

S

T

P

P

E

E

R

K

R

P

R

K

G

A

V

V

T

T

I

H

S

S

G

G

N

N

H

H

A

G

Q

Q

A

A

V

L

A

T

L

Y

V

A

G

A

R

K

E

L

L

E

G

G

I

I

D

P

T

A

T

Y

V

I

L

V

M

V

P

P

A

Q

N

T

A

A

P

P

E

N

I
x
C

K

K

R

K

R

L

A

A

V

I

E

Q

G

A

Y

Y

G

G

A

A

T

S

I

I

V

V

T

Y

Y

C

D

D

P

P

A

S

T

D

T

E

R

S

R

R

E

E

E

K

A

V

L

T

-

Q

R

R

A

I

V

M

D

Q

G

E

G

T

E

E

R

G

V

I

F

L

I

V

P

H

P

P

F

N

D

Q

H

E

W

P

D

A

V

V

I

I

A

A

G

G

Q

Q

G

G

T

T

A

I

G

A

L

L

E

E

L

V

A

L

Q

N

A

Q

V

V

S

P

H

L

L

V

D

D

M

A

L

L

L

V

V

V

P

P

C

V

G

G

L

M

L

V

S

A

G

G

C

I

A

A

V

I

A

T

V

I

K

K

G

A

Y

L

F

K

P

P

R

S

C

V

R

K

V

V

V

Y

G

A

V

A

E

E

P

P

E

S

S

N

A

A

D

D

A

C

T

Y

R

Q

S

S

F

K

R

L

M

K

G

G

T

E

L

L

Q

T

-

P

E

N

V

L

H

H

N

P

P

P

P

E

T

T

I

I

A

A

D

D

G

G

V

V

R

K

T

S

P

-

R

S

L

I

G

G

D

L

I

N

T

T

F

W

P

P

L

I

V

I

L

R

A

D

H

L

V

V

D

D

M

V

R

F

T

T

V

V

S

T

E

E

E

D

A

E

I

I

R

K

E

Y

A

A

V

T

R

Q

F

L

L

V

F

W

Y

G

R

R

M

M

K

K

L

L

V

L

V

I

E

E

P

P

S

T

G

A

A

G

L

V

G

A

L

L

A

A

L

V

L

L

S

S

-

Q

-

H

-

F

G

Q

A

T

V

V

K

S

P

P

-

E

T

V

G

K

D

N

V

V

G

C

I

I

L

V

L

L

S

S

G

G

N

N

V

V

D

D

C113 (≠ I82) modified: S-nitrosocysteine; mutation to S: Abolishes S-nitrosylation.

Q76EQ0 Serine racemase; D-serine ammonia-lyase; D-serine dehydratase; L-serine ammonia-lyase; L-serine dehydratase; EC 5.1.1.18; EC 4.3.1.18; EC 4.3.1.17 from Rattus norvegicus (Rat) (see paper)
41% identity, 96% coverage: 6:289/296 of query aligns to 34:325/333 of Q76EQ0

query
sites
Q76EQ0

L

L

D

N

K

Q

M

I

L

A

G

G

A

R

R

N

F

L

H

F

F

F

K

K

C

C

E

E

N

L

L

F

Q

Q

R

K

I

T

G

G

A

S

F

F

K
|
K

F

I

R

R

G

G

A

A

F

L

N

N

A

A

L

I

S

R

R

G

L

L

S

I

P

P

-

D

-

T

-

L

E

E

E

G

R

K

R

P

R

K

G

A

V

V

T

T

I

H

S

S

G

G

N
|
N

H

H

A

G

Q

Q

A

A

V

L

A

T

L

Y

V

A

G

A

R

K

E

L

L

E

G

G

I

I

D

P

T

A

T

Y

V

I

L

V

M

V

P

P

A

Q

N

T

A

A

P

P

E

N

I

C

K

K

R

K

R

L

A

A

V

I

E

Q

G

A

Y

Y

G

G

A

A

T

S

I

I

V

V

T

Y

Y

S

D

E

P

P

A

S

T

D

T

E

R

S

R

R

E

E

E

N

-

V

A

A

L

Q

R

R

A

I

V

I

D

Q

G

E

G

T

E

E

R

G

V

I

F

L

I

V

P

H

P

P

F
x
N

D

Q

H

E

W

P

D

A

V

V

I

I

A

A

G

G

Q

Q

G

G

T

T

A

I

G

A

L

L

E

E

L

V

A

L

Q

N

A

Q

V

V

S

P

H

L

L

V

D

D

M

A

L

L

L

V

V

V

P

P

C

V

G
|
G

L
x
M

L

V

S

A

G

G

C

I

A

A

V

I

A

T

V

I

K

K

G

T

Y

L

F

K

P

P

R

S

C

V

R

K

V

V

V

Y

G

A

V

A

E
|
E

P

P

E

S

S

N

A
|
A

D

D

D
|
D

A

C

T

Y

R

Q

S

S

F

K

R

L

M

K

G

G

T

E

L

L

Q

T

-

P

E

N

V

L

H

H

N

P

P

P

P

E

T

T

I

I

A

A

D

D

G

G

V

V

R

K

T

S

P

-

R

S

L

I

G

G

D

L

I

N

T

T

F

W

P

P

L

I

V

I

L

R

A

D

H

L

V

V

D

D

M

V

R

F

T

T

V

V

S

T

E

E

E

D

A

E

I

I

R

K

E

Y

A

A

V

T

R

Q

F

L

L

V

F

W

Y

E

R

R

M

M

K

K

L

L

V

L

V

I

E

E

P

P

S

T

G

A

A

G

L

V

G

G

L

L

A

A

L

V

L

L

S

S

-

Q

-

H

-

F

G

Q

A

T

V

V

K

S

P

P

-

E

T

V

G

K

D

N

V

I

G

C

I

I

L

V

L

L

S
|
S

G

G

N

N

V

V

D

D

G

L

Q

T

T

S

M

L

A

S

G

W

I

V

K56 (= K28) modified: N6-(pyridoxal phosphate)lysine
N86 (= N55) binding pyridoxal 5'-phosphate
N154 (≠ F122) binding pyridoxal 5'-phosphate
G185 (= G153) binding pyridoxal 5'-phosphate
G186 (= G154) binding pyridoxal 5'-phosphate
G187 (= G155) binding pyridoxal 5'-phosphate
G188 (= G156) binding pyridoxal 5'-phosphate
M189 (≠ L157) binding pyridoxal 5'-phosphate
E210 (= E178) binding Mn(2+)
A214 (= A182) binding Mn(2+)
D216 (= D184) binding Mn(2+)
S313 (= S277) binding pyridoxal 5'-phosphate

Query Sequence

>WP_084076500.1 NCBI__GCF_900129305.1:WP_084076500.1
MGCRTLDKMLGARFHFKCENLQRIGAFKFRGAFNALSRLSPEERRRGVVTISSGNHAQAV
ALVGRELGIDTTVLMPANAPEIKRRAVEGYGATIVTYDPATTRREEALRAVDGGERVFIP
PFDHWDVIAGQGTAGLELAQAVSHLDMLLVPCGGGGLLSGCAVAVKGYFPRCRVVGVEPE
SADDATRSFRMGTLQEVHNPPTIADGVRTPRLGDITFPLVLAHVDDMRTVSEEAIREAVR
FLFYRMKLVVEPSGALGLAALLSGAVKPTGDVGILLSGGNVDGQTMAGILSESPGS

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory