SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_084198362.1 NCBI__GCF_002869505.1:WP_084198362.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

3gqtC Crystal structure of glutaryl-coa dehydrogenase from burkholderia pseudomallei with fragment (1,4-dimethyl-1,2,3,4- tetrahydroquinoxalin-6-yl)methylamine (see paper)
73% identity, 98% coverage: 9:393/393 of query aligns to 3:385/385 of 3gqtC

query
sites
3gqtC

L

F

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H

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W

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D

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F

L

L

L

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Y

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C

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D

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Y

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C

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R

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F

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I

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K

L

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L

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G

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L

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A

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V

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P

E

E

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N

L

I

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L

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F

active site: L135 (= L140), T136 (= T141), A250 (= A252), E365 (= E373), R377 (= R385)
binding 1-(1,4-dimethyl-1,2,3,4-tetrahydroquinoxalin-6-yl)methanamine: W166 (= W171), K210 (= K212), L213 (= L215), T218 (= T220), Y364 (= Y372)

3eonC 2.55a crystal structure of native glutaryl-coa dehydrogenase from burkholderia pseudomallei in complex with a small molecule (see paper)
73% identity, 98% coverage: 9:392/393 of query aligns to 3:382/382 of 3eonC

query
sites
3eonC

L

F

N

H

W

W

E

D

D

D

P

P

F

L

L

L

E

D

H

Q

Q

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L

T

A

D

D

E

D

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E

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R

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M

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V

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Y

Y

A

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G

K

K

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L

A

A

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T

E

E

A

A

F

F

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R

A

H

E

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T

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D

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A

A

A

I

I

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F

N

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M

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G

E

E

L

I

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G

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L

G

G

S

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T

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I

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E

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Y

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C

G

P

P

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G

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L

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D

Y

Y

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S

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Y

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G

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I

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A

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D

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S

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C

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N

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C

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M

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A

active site: L135 (= L140), T136 (= T141), A244 (= A252), E363 (= E373), R375 (= R385)
binding (3,5-difluorophenyl)methanol: R94 (= R99), S95 (= S100), I248 (= I256), K322 (= K330), Y362 (= Y372)

3gncA Crystal structure of glutaryl-coa dehydrogenase from burkholderia pseudomallei with fragment 6421 (see paper)
72% identity, 98% coverage: 9:392/393 of query aligns to 4:380/380 of 3gncA

query
sites
3gncA

L

F

N

H

W

W

E

D

D

D

P

P

F

L

L

L

E

D

H

Q

Q

Q

L

L

T

A

D

D

E

D

Q

E

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R

M

M

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V

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R

Q

D

S

A

A

A

R

H

Q

A

Y

Y

A

A

Q

Q

D

G

K

K

L

L

A

A

P

P

R

R

V

V

R

T

E

E

A

A

F

F

R

R

A

H

E

E

H

T

T

T

D

D

R

A

A

A

I

I

F

F

N

R

E

E

M

M

G

G

E

E

L

I

G

G

L

L

G

G

S

P

T

T

I

I

-

P

D

E

G

Q

Y

Y

G

G

C

G

P

P

G

G

V

L

D

D

Y

Y

I

V

S

S

Y

Y

G

G

L

L

V

I

A

A

R

R

E

E

V

V

E

E

R

R

V

V

D

D

S

S

G

G

Y

Y

R
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R

S
|
S

M

M

S

S

V

V

Q

Q

S

S

L

L

V

V

M

M

Y

V

P

P

I

I

W

F

A

E

Y

F

G

G

N

S

E

D

A

A

Q

Q

R

K

E

E

K

K

Y

Y

L

L

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P

K

K

L

L

A

A

T

T

G

G

E

E

W

W

V

I

G

G

C

C

F

F

G

G

L
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L

T
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T

E

E

P

P

D

-

H

-

G

-

S

-

D

-

P

-

G

-

S

-

M

M

V

V

T

T

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R

A

A

R

R

S

K

V

V

E

P

G

G

Y

Y

S

S

L

L

S

S

G

G

A

S

K

K

M

M

W

W

I

I

T

T

N

N

S

S

P

P

F

I

A

A

D

D

V

V

F

F

V

V

W

W

A

A

K

K

T

L

D

D

D

E

G

D

K

R

-

D

-

E

I

I

R

R

G

G

F

F

V

I

L

L

E

E

K

K

G

G

M

C

E

K

G

G

L

L

S

S

A

A

P

P

K

A

I

I

E

H

G

G

K

K

L

V

A

G

L

L

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R

A

A

S

S

V

I

T

T

G

G

E

E

I

I

V

V

M

L

D

D

E

E

V

A

F

F

V

V

P

P

E

E

N

N

L

I

L

L

P

P

G

H

V

V

E

K

G

G

L

L

K

R

G

G

P

P

F

F

G

T

C

C

L

L

N

N

N

S

A
|
A

R

R

Y

Y

G

G

I
|
I

A

A

W

W

G

G

A

A

I

L

G

G

A

A

E

E

T

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C

C

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W

H

H

A

I

A

A

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R

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Y

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L

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D

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R

K

K

Q

Q

F

F

G

G

V

R

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P

L

L

A

A

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A

K

N

Q

Q

L

L

V

I

Q

Q

L

K

K

K

L

L

A

A

D

D

M

M

Q

Q

T

T

E

E

I

I

S

T

L

L

A

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L

L

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Q

G

G

C

V

L

L

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R

A

L

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G

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R

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M

K

D

D

A

E

G

G

E

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I

A

A

A

P

V

E

E

L

I

I

T

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S

L

I

I

M

K

K

R

R

N

N

S

S

C

C

G

G

K

K

A

A

L

L

D

D

I

I

A

A

R

R

T

L

A

A

R

R

D

D

M

M

L

L

G

G

N

N

G

G

I

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-

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D

E

E

Y

F

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M

A

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R

H

H

M

L

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V

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N

L

L

E

E

V

V

V
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V

N

N

T

T

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Y

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E

G

G

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T

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H

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A

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A

active site: L136 (= L140), T137 (= T141), A242 (= A252), E361 (= E373), R373 (= R385)
binding 1-(1-methylethyl)-1H-benzimidazole-2-sulfonic acid: R95 (= R99), S96 (= S100), I246 (= I256), V357 (= V369), Y360 (= Y372), E361 (= E373)

3d6bC 2.2 a crystal structure of glutaryl-coa dehydrogenase from burkholderia pseudomallei (see paper)
72% identity, 98% coverage: 9:392/393 of query aligns to 3:377/377 of 3d6bC

query
sites
3d6bC

L

F

N

H

W

W

E

D

D

D

P

P

F

L

L

L

E

D

H

Q

Q

Q

L

L

T

A

D

D

E

D

Q

E

R

R

M

M

V

V

Q

R

Q

D

S

A

A

A

R

H

Q

A

Y

Y

A

A

Q

Q

D

G

K

K

L

L

A

A

P

P

R

R

V

V

R

T

E

E

A

A

F

F

R

R

A

H

E

E

H

T

T

T

D

D

R

A

A

A

I

I

F

F

N

R

E

E

M

M

G

G

E

E

L

I

G

G

L

L

G

G

S

P

T

T

I

I

-

P

D

E

G

Q

Y

Y

G

G

C

G

P

P

G

G

V

L

D

D

Y

Y

I

V

S

S

Y

Y

G

G

L

L

V

I

A

A

R

R

E

E

V

V

E

E

R

R

V

V

D

D

S

S

G

G

Y

Y

R

R

S

S

M

M

S

S

V

V

Q

Q

S

S

L

L

V

V

M

M

Y

V

P

P

I

I

W

F

A

E

Y

F

G

G

N

S

E

D

A

A

Q

Q

R

K

E

E

K

K

Y

Y

L

L

P

P

K

K

L

L

A

A

T

T

G

G

E

E

W

W

V

I

G

G

C

C

F

F

G

G

L
|
L

T
|
T

E

E

P

P

D

N

H

H

G

G

S

-

D

-

P

-

G

G

S

S

M

M

V

V

T

T

R

R

A

A

R

R

S

K

V

V

E

P

G

G

Y

Y

S

S

L

L

S

S

G

G

A

S

K

K

M

M

W

W

I

I

T

T

N

N

S

S

P

P

F

I

A

A

D

D

V

V

F

F

V

V

W

W

A

A

K

K

T

L

D

D

-

E

D

D

G

G

K

R

-

D

-

E

I

I

R

R

G

G

F

F

V

I

L

L

E

E

K

K

G

G

M

C

E

K

G

G

L

L

S

S

A

A

P

P

K

A

I

I

E

H

G

G

K

K

L

V

A

G

L

L

R

R

A

A

S

S

V

I

T

T

G

G

E

E

I

I

V

V

M

L

D

D

E

E

V

A

F

F

V

V

P

P

E

E

N

N

L

I

L

L

P

P

G

H

V

V

E

K

G

G

L

L

K

R

G

G

P

P

F

F

G

T

C

C

L

L

N

N

N

S

A
|
A

R

R

Y

Y

G

G

I
|
I

A

A

W

W

G

G

A

A

I

L

G

G

A

A

E

E

T

S

C

C

W

W

H

H

A

I

A

A

R

R

E

Q

Y

Y

T

V

L

L

D

D

R

R

K

K

Q

Q

F

-

G

-

V

-

P

P

L

L

A

A

S

A

K

N

Q

Q

L

L

V

I

Q

Q

L

K

K

K

L

L

A

A

D

D

M

M

Q

Q

T

T

E

E

I

I

S

T

L

L

A

G

L

L

Q

Q

G

G

C

V

L

L

R

R

A

L

G

G

Q

R

L

M

M

K

D

D

A

E

G

G

E

T

I

A

A

A

P

V

E

E

L

I

I

T

S

S

L

I

I

M

K

K

R

R

N

N

S

S

C

C

G

G

K

K

A

A

L

L

D

D

I

I

A

A

R

R

T

L

A

A

R

R

D

D

M

M

L

L

G

G

G

-

N

-

G

-

I

-

S

-

D

-

E

-

Y

F

P

G

I

V

M

A

R

R

H

H

M

L

V

V

N

N

L

L

E

E

V

V

N

N

T

T

Y
|
Y

E
|
E

G

G

T

T

H

H

D

D

V

I

H

H

A

A

L

L

I

I

L

L

G

G

R
|
R

T

A

Q

Q

T

T

D

G

I

I

P

Q

A

A

active site: L135 (= L140), T136 (= T141), A247 (= A252), E358 (= E373), R370 (= R385)
binding methyl thiophene-2-carboxylate: I251 (= I256), Y357 (= Y372), E358 (= E373)

2r0nA The effect of a glu370asp mutation in glutaryl-coa dehydrogenase on proton transfer to the dienolate intermediate (see paper)
66% identity, 98% coverage: 8:393/393 of query aligns to 1:388/390 of 2r0nA

query
sites
2r0nA

K

E

L

F

N

D

W

W

E

Q

D

D

P

P

F

L

L

V

L

L

E

E

H

E

Q

Q

L

L

T

T

D

T

E

D

Q

E

R

I

M

L

V

I

Q

R

Q

D

S

T

A

F

R

R

Q

T

Y

Y

A

C

Q

Q

D

E

K

R

L

L

A

M

P

P

R

R

V

I

R

L

E

L

A

A

F

N

R

R

A

N

E

E

H

V

T

F

D

H

R

R

A

E

I

I

F

I

N

S

E

E

M

M

G

G

E

E

L

L

G

G

L

V

L

L

G

G

S

P

T

T

I

I

D

K

G

G

Y

Y

G

G

C

C

P

A

G

G

V

V

D

S

Y

S

I

V

S

A

Y

Y

G

G

L

L

V

L

A

A

R

R

E

E

V

L

E

E

R

R

V

V

D

D

S

S

G

G

Y

Y

R
|
R

S
|
S

M

A

M

M

S

S

V
|
V

Q

Q

S

S

L

L

V

V

M

M

Y

H

P

P

I

I

W

Y

A

A

Y

Y

G

G

N

S

E

E

A

E

Q

Q

R

R

E

Q

K

K

Y

Y

L

L

P

P

K

Q

L

L

A

A

T

K

G

G

E

E

W

L

V

L

G

G

C

C

F
|
F

G

G

L
|
L

T
|
T

E

E

P

P

D

N

H

S

G
|
G

S
|
S

D

D

P
|
P

G

S

S

S

M

M

V

E

T

T

R

R

A

A

R

H

-

Y

-

N

S

S

V

S

E

N

G

K

G

S

Y

Y

S

T

L

L

S

N

G

G

A

T

K

K

M

T

W
|
W

I
|
I

T
|
T

N

N

S

S

P

P

F

M

A

A

D

D

V

L

F

F

V

V

W

W

A

A

K

R

T

C

D

E

D

D

G

G

K

C

I

I

R

R

G

G

F

F

V

L

L

L

E

E

K

K

G

G

M

M

E

R

G

G

L

L

S

S

A

A

P

P

K

R

I

I

E

Q

G

G

K

K

L

F

A

S

L

L

R

R

A

A

S

S

V

A

T

T

G

G

E

M

I

I

V

I

M

M

D

D

E

G

V

V

F

E

V

V

P

P

E

E

N

N

L

V

L

L

P

P

G

G

V

A

E

S

G

S

L

L

K
x
G

G

G

P

P

F
|
F

G

G

C

C

L
|
L

N
|
N

N

N

A
|
A

R

R

Y

Y

G

G

I

I

A

A

W

W

G

G

A

V

I

L

G

G

A

A

A

S

E

E

T

F

C

C

W

L

H

H

A

T

A

A

R

R

E

Q

Y

Y

T

A

L

L

D

D

R

R

K

M

Q

Q

F

F

G

G

V

V

P

P

L

L

A

A

S

R

K

N

Q

Q

L

L

V

I

Q

Q

L

K

K

K

L

L

A

A

D

D

M

M

Q

L

T

T

E

E

I

I

S

T

L

L

A

G

L

L

Q

H

G

A

C

C

L

L

R

Q

A

L

G

G

Q

R

L

L

M

K

D

D

A

Q

G

D

E

K

I

A

A

A

P
|
P

E

E

L

M

I

V

S

S

L

L

I

L

K

K

R

R

N

N

S

N

C

C

G

G

K

K

A

A

L

L

D

D

I

I

A

A

R

R

T

Q

A

A

R

R

D

D

M

M

L

L

G

G

N

N

G

G

I

I

S

S

D

D

E

E

Y

Y

P

H

I

V

M

I

R

R

H

H

M

A

V

M

N

N

L

L

E

E

V

A

V

V

N

N

T

T

Y
|
Y

E
|
E

G

G

T
|
T

H

H

D
|
D

V

I

H

H

A

A

L

L

I
|
I

L

L

G

G

R
|
R

T

A

Q

I

T

T

D

G

I

I

P

Q

A

A

F

F

active site: L133 (= L140), T134 (= T141), A247 (= A252), E368 (= E373), R380 (= R385)
binding flavin-adenine dinucleotide: F131 (= F138), L133 (= L140), T134 (= T141), G139 (= G146), S140 (= S147), W166 (= W171), I167 (= I172), T168 (= T173), Y367 (= Y372), T370 (= T375), D372 (= D377)
binding 3-thiaglutaryl-CoA: R92 (= R99), S93 (= S100), V97 (= V104), P142 (= P149), G238 (≠ K243), F241 (= F246), L244 (= L249), N245 (= N250), P318 (= P323), Y367 (= Y372), E368 (= E373), I377 (= I382)

1sirA The crystal structure and mechanism of human glutaryl-coa dehydrogenase (see paper)
66% identity, 98% coverage: 8:393/393 of query aligns to 1:388/390 of 1sirA

query
sites
1sirA

K

E

L

F

N

D

W

W

E

Q

D

D

P

P

F

L

L

V

L

L

E

E

H

E

Q

Q

L

L

T

T

D

T

E

D

Q

E

R

I

M

L

V

I

Q

R

Q

D

S

T

A

F

R

R

Q

T

Y

Y

A

C

Q

Q

D

E

K

R

L

L

A

M

P

P

R

R

V

I

R

L

E

L

A

A

F

N

R

R

A

N

E

E

H

V

T

F

D

H

R

R

A

E

I

I

F

I

N

S

E

E

M

M

G

G

E

E

L

L

G

G

L

V

L

L

G

G

S

P

T

T

I

I

D

K

G

G

Y

Y

G

G

C

C

P

A

G

G

V

V

D

S

Y

S

I

V

S

A

Y

Y

G

G

L

L

V

L

A

A

R

R

E

E

V

L

E

E

R

R

V

V

D

D

S

S

G

G

Y

Y

R

R

S
|
S

M

A

M

M

S

S

V

V

Q

Q

S

S

L

L

V

V

M

M

Y

H

P

P

I

I

W

Y

A

A

Y

Y

G

G

N

S

E

E

A

E

Q

Q

R

R

E

Q

K

K

Y

Y

L

L

P

P

K

Q

L

L

A

A

T

K

G

G

E

E

W

L

V

L

G

G

C

C

F
|
F

G

G

L
|
L

T
|
T

E

E

P

P

D

N

H

S

G
|
G

S
|
S

D

D

P

P

G

S

S

S

M

M

V

E

T

T

R

R

A

A

R

H

-

Y

-

N

S

S

V

S

E

N

G

K

G

S

Y

Y

S

T

L

L

S

N

G

G

A

T

K

K

M

T

W
|
W

I
|
I

T
|
T

N

N

S

S

P

P

F

M

A

A

D

D

V

L

F

F

V

V

W

W

A

A

K

R

T

C

D

E

D

D

G

G

K

C

I

I

R

R

G

G

F

F

V

L

L

L

E

E

K

K

G

G

M

M

E

R

G

G

L

L

S

S

A

A

P

P

K

R

I

I

E

Q

G

G

K

K

L

F

A

S

L

L

R

R

A

A

S

S

V

A

T

T

G

G

E

M

I

I

V

I

M

M

D

D

E

G

V

V

F

E

V

V

P

P

E

E

N

N

L

V

L

L

P

P

G

G

V

A

E

S

G

S

L

L

K

G

G

G

P

P

F
|
F

G
|
G

C

C

L
|
L

N
|
N

N

N

A
|
A

R
|
R

Y

Y

G

G

I

I

A

A

W

W

G

G

A

V

I

L

G

G

A

A

A

S

E

E

T

F

C

C

W

L

H

H

A

T

A

A

R

R

E

Q

Y

Y

T

A

L

L

D

D

R

R

K

M

Q

Q

F

F

G

G

V

V

P

P

L

L

A

A

S

R

K

N

Q

Q

L

L

V

I

Q

Q

L

K

K

K

L

L

A

A

D

D

M

M

Q

L

T

T

E

E

I

I

S

T

L

L

A

G

L

L

Q

H

G

A

C

C

L

L

R

Q

A

L

G

G

Q

R

L

L

M

K

D

D

A

Q

G

D

E

K

I

A

A

A

P
|
P

E

E

L

M

I

V

S

S

L

L

I

L

K

K

R

R

N

N

S

N

C

C

G

G

K

K

A

A

L

L

D

D

I

I

A

A

R

R

T

Q

A

A

R

R

D

D

M

M

L

L

G

G

N

N

G

G

I

I

S

S

D

D

E

E

Y

Y

P

H

I

V

M

I

R

R

H

H

M

A

V

M

N

N

L

L

E

E

V

A

V

V

N

N

T

T

Y
|
Y

E
|
E

G

G

T
|
T

H

H

D

D

V

I

H

H

A

A

L

L

I

I

L

L

G

G

R
|
R

T

A

Q

I

T

T

D

G

I

I

P

Q

A

A

F

F

active site: L133 (= L140), T134 (= T141), A247 (= A252), E368 (= E373), R380 (= R385)
binding flavin-adenine dinucleotide: F131 (= F138), L133 (= L140), T134 (= T141), G139 (= G146), S140 (= S147), W166 (= W171), I167 (= I172), T168 (= T173), Y367 (= Y372), T370 (= T375)
binding s-4-nitrobutyryl-coa: S93 (= S100), S140 (= S147), F241 (= F246), G242 (= G247), L244 (= L249), N245 (= N250), R248 (= R253), P318 (= P323), Y367 (= Y372), E368 (= E373), R380 (= R385)

2r0mA The effect of a glu370asp mutation in glutaryl-coa dehydrogenase on proton transfer to the dienolate intermediate (see paper)
66% identity, 98% coverage: 8:393/393 of query aligns to 1:388/390 of 2r0mA

query
sites
2r0mA

K

E

L

F

N

D

W

W

E

Q

D

D

P

P

F

L

L

V

L

L

E

E

H

E

Q

Q

L

L

T

T

D

T

E

D

Q

E

R

I

M

L

V

I

Q

R

Q

D

S

T

A

F

R

R

Q

T

Y

Y

A

C

Q

Q

D

E

K

R

L

L

A

M

P

P

R

R

V

I

R

L

E

L

A

A

F

N

R

R

A

N

E

E

H

V

T

F

D

H

R

R

A

E

I

I

F

I

N

S

E

E

M

M

G

G

E

E

L

L

G

G

L

V

L

L

G

G

S

P

T

T

I

I

D

K

G

G

Y

Y

G

G

C

C

P

A

G

G

V

V

D

S

Y

S

I

V

S

A

Y

Y

G

G

L

L

V

L

A

A

R

R

E

E

V

L

E

E

R

R

V

V

D

D

S

S

G

G

Y

Y

R

R

S

S

M

A

M

M

S

S

V

V

Q

Q

S

S

L
|
L

V

V

M

M

Y

H

P

P

I

I

W

Y

A

A

Y

Y

G

G

N

S

E

E

A

E

Q

Q

R

R

E

Q

K

K

Y

Y

L

L

P

P

K

Q

L

L

A

A

T

K

G

G

E

E

W

L

V

L

G

G

C

C

F
|
F

G

G

L
|
L

T
|
T

E

E

P

P

D

N

H

S

G
|
G

S
|
S

D

D

P

P

G

S

S

S

M

M

V

E

T

T

R

R

A

A

R

H

-

Y

-

N

S

S

V

S

E

N

G

K

G

S

Y

Y

S

T

L

L

S

N

G

G

A

T

K

K

M

T

W
|
W

I
|
I

T
|
T

N

N

S

S

P

P

F

M

A

A

D

D

V

L

F

F

V

V

W

W

A

A

K

R

T

C

D

E

D

D

G

G

K

C

I

I

R

R

G

G

F

F

V

L

L

L

E

E

K

K

G

G

M

M

E

R

G

G

L

L

S

S

A

A

P

P

K

R

I

I

E

Q

G

G

K

K

L

F

A

S

L
|
L

R

R

A

A

S

S

V

A

T

T

G

G

E

M

I

I

V

I

M

M

D

D

E

G

V

V

F

E

V

V

P

P

E

E

N

N

L

V

L

L

P

P

G

G

V

A

E

S

G

S

L

L

K

G

G

G

P

P

F

F

G

G

C

C

L

L

N

N

A
|
A

R

R

Y

Y

G

G

I

I

A

A

W

W

G

G

A

V

I

L

G

G

A

A

A

S

E

E

T

F

C

C

W

L

H

H

A

T

A

A

R

R

E

Q

Y

Y

T

A

L

L

D

D

R

R

K

M

Q

Q

F

F

G

G

V

V

P

P

L

L

A

A

S

R

K

N

Q

Q

L

L

V

I

Q

Q

L

K

K

K

L

L

A

A

D

D

M

M

Q

L

T

T

E

E

I

I

S

T

L

L

A

G

L

L

Q

H

G

A

C

C

L

L

R

Q

A

L

G

G

Q

R

L

L

M

K

D

D

A

Q

G

D

E

K

I

A

A

A

P

P

E

E

L

M

I

V

S

S

L

L

I

L

K

K

R

R

N

N

S

N

C

C

G

G

K

K

A

A

L

L

D

D

I

I

A

A

R

R

T

Q

A

A

R

R

D

D

M

M

L

L

G

G

N

N

G

G

I

I

S

S

D

D

E

E

Y

Y

P

H

I

V

M

I

R

R

H

H

M

A

V

M

N

N

L

L

E

E

V

A

V

V

N

N

T

T

Y
|
Y

E
x
D

G

G

T
|
T

H

H

D

D

V

I

H

H

A

A

L

L

I

I

L

L

G

G

R
|
R

T

A

Q

I

T

T

D

G

I

I

P

Q

A

A

F

F

active site: L133 (= L140), T134 (= T141), A247 (= A252), D368 (≠ E373), R380 (= R385)
binding 4-nitrobutanoic acid: L101 (= L108), Y367 (= Y372), D368 (≠ E373)
binding flavin-adenine dinucleotide: F131 (= F138), L133 (= L140), T134 (= T141), G139 (= G146), S140 (= S147), W166 (= W171), I167 (= I172), T168 (= T173), L210 (= L215), Y367 (= Y372), T370 (= T375)

3sf6A Crystal structure of glutaryl-coa dehydrogenase from mycobacterium smegmatis (see paper)
48% identity, 97% coverage: 12:393/393 of query aligns to 5:386/387 of 3sf6A

query
sites
3sf6A

E

D

D

D

P

L

F

I

L

G

L

I

E

N

H

A

Q

V

L

L

T

S

D

A

E

E

Q

E

R

R

M

E

V

I

Q

R

Q

D

S

T

A

V

R

R

Q

S

Y

V

A

V

Q

Q

D

R

K

R

L

I

A

K

P

P

R

H

V

I

R

A

E

S

A

W

F

Y

R

E

-

D

A

G

E

E

H

L

T

P

D

A

R

R

A

E

I

L

F

A

N

V

E

E

M

L

G

G

E

E

L

L

G

G

L

L

G

G

S

M

T

H

I

L

D

K

G

G

Y

Y

G

G

C

C

P

A

G

G

V

M

D

S

Y

A

I

V

S

A

Y

Y

G

G

L

L

V

A

A

C

R

L

E

E

V

L

E

E

R

A

V

G

D

D

S

S

G

G

Y

I

R

R

S

S

M

L

M

V

S

S

V

V

Q

Q

S

G

S

S

L

L

V

A

M

M

Y

Y

P

A

I

I

W

H

A

A

Y

F

G

G

N

S

E

D

A

E

Q

Q

R

K

E

D

K

Q

Y

W

L

L

P

P

K

D

L

M

A

A

T

S

G

G

E

H

W

R

V

I

G

G

C

C

F
|
F

G

G

L
|
L

T
|
T

E

E

P

P

D

D

H

H

G
|
G

S
|
S

D

D

P

P

G

A

S

G

M

M

V

R

T

T

R

R

A

A

R

T

S

R

V

S

E

G

G

D

Y

W

S

I

L

L

S

T

G

G

A

T

K

K

M

M

W
|
W

I
|
I

T
|
T

N

N

S

G

P

S

F

V

A

A

D

D

V

V

F

A

V

V

W

W

A

A

K

R

T

T

D

D

D

E

G

G

K

-

I

I

R

R

G

G

F

F

V

V

L

V

E

P

K

T

G

D

M

T

E

P

G

G

L

F

S

T

A

A

P

N

K

T

I

I

E

K

G

S

K

K

L

M

A

S

L

L

R

R

A

A

S

S

V

V

T

T

G

S

E

E

I

L

V

V

M

L

D

D

E

G

V

V

F

R

V

L

P

P

E

D

E

S

N

A

L

R

L

L

P

P

G

G

V

A

E

T

G

S

L

L

K

G

G

A

P

P

F

L

G

R

C

C

L

L

N

N

N

E

A
|
A

R

R

Y

F

G

G

I

I

A

V

W

F

G

G

A

A

I

L

G

G

A

A

E

R

T

D

C

C

W

L

H

E

A

T

A

A

R

L

E

A

Y

Y

T

A

L

C

D

S

R

R

K

E

Q

Q

F

F

G

D

V

R

P

P

L

I

A

G

S

G

K

F

Q

Q

L

L

V

T

Q

Q

L

Q

K

K

L

L

A

A

D

D

M

M

Q

T

T

L

E

E

I

Y

S

G

L

K

A

G

L

F

Q

L

G

L

C

A

L

L

R

H

A

L

G

G

Q

R

L

Q

M

K

D

D

A

A

G

G

E

E

I

L

A

A

P

P

E

E

L

Q

I

V

S

S

L

L

I

G

K

K

R

L

N

N

S

N

C

V

G

R

K

E

A

A

L

I

D

E

I

I

A

A

R

R

T

T

A

A

R

R

D

T

M

V

L

L

G

G

G

A

N

S

G

G

I

I

S

T

D

G

E

E

Y

Y

P

P

I

V

M

M

R

R

H

H

M

A

V

N

N

N

L

L

E

E

V
x
S

V

V

N

L

T
|
T

Y
|
Y

E
|
E

G

G

T
|
T

H

S

D
x
E

V
x
M

H

H

A

T

L

L

I

I

L

I

G

G

R
x
Q

T

A

Q

L

T

T

D

G

I

V

P

G

A

A

F

F

active site: L134 (= L140), T135 (= T141), A245 (= A252), E366 (= E373), Q378 (≠ R385)
binding dihydroflavine-adenine dinucleotide: F132 (= F138), L134 (= L140), T135 (= T141), G140 (= G146), S141 (= S147), W165 (= W171), I166 (= I172), T167 (= T173), S361 (≠ V368), T364 (= T371), Y365 (= Y372), T368 (= T375), E370 (≠ D377), M371 (≠ V378)

2ebaA Crystal structure of the putative glutaryl-coa dehydrogenase from thermus thermophilus
50% identity, 97% coverage: 13:393/393 of query aligns to 3:380/380 of 2ebaA

query
sites
2ebaA

D

D

P

F

F

Y

L

A

L

L

E

E

H

D

Q

L

L

L

T

T

D

P

E

E

Q

E

R

K

M

E

V

V

Q

Q

Q

K

S

A

A

A

R

R

Q

R

Y

F

A

L

Q

E

D

K

K

E

L

A

A

L

P

P

R

H

V

I

R

R

E

D

A

W

F

W

R

E

A

E

E

G

H

V

T

F

D

P

R

T

A

H

I

L

F

I

N

P

E

R

M

F

G

A

E

E

L

L

G

G

L

F

L

L

G

G

S

P

T

T

I

L

D

P

G

P

-

E

Y

Y

G

G

C

G

P

A

G

G

V

V

D

S

Y

S

I

A

S

A

Y

Y

G

G

L

L

V

I

A

C

R

Y

E

E

V

L

E

E

R

R

V

V

D

D

S

S

G

G

Y

L

R

R

S

S

M

F

M

V

S

S

V

V

Q

Q

S

S

L

L

V

V

M

M

Y

Y

P

P

I

I

W

Y

A

A

Y

Y

G

G

N

S

E

E

A

E

Q

Q

R

K

E

R

K

E

Y

F

L

L

P

P

K

K

L

L

A

A

T

R

G

G

E

E

W

M

V

V

G

G

C

C

F

F

G

G

L
|
L

T
|
T

E

E

P

P

D

D

H
x
G

G
|
G

S
|
S

D

D

P

P

-

Y

G

G

S

N

M

M

V

K

T

T

R

R

A

A

R

R

S

-

V

-

E

R

G

D

G

T

Y

W

S

V

L

L

S

N

G

G

A

T

K

K

M

M

W
|
W

I

I

T
|
T

N

N

S

G

P

N

F

L

A

A

D

H

V

L

F

A

V

V

V

I

W

W

A

A

K

K

T

D

D

E

D

-

G

-

K

-

I

V

R

L

G

G

F

F

V

L

L

V

E

P

K

T

G

D

M

T

E

P

G

G

L

F

S

Q

A

A

P

R

K

E

I

V

E

K

G

R

K

K

L

M

A

S

L

L

R

R

A

A

S

S

V

V

T

T

G

S

E

E

I

L

V

V

M

L

D

E

E

E

V

V

F

R

V

V

P

P

E

E

E

S

N

L

L

R

L

L

P

P

G

K

V

A

E

L

G

G

L

L

K

K

G

A

P

P

F

L

G

S

C

C

L

L

N

T

N

Q

A
|
A

R

R

Y

F

G

G

I

I

A

A

W

W

G

G

A

A

I

M

G

G

A

A

A

L

E

E

T

A

C

V

W

Y

H

E

A

E

A

A

R

V

E

A

Y

F

T

A

L

K

D

S

R
|
R

K

S

Q

T

F

F

G

G

V

E

P

P

L
|
L

A

A

S

K

K
|
K

Q

Q

L

L

V

V

Q

Q

L

A

K

K

L

L

A

A

D

E

M

M

Q

L

T

A

E

W

I

H

S

T

L

E

A

G

L

L

Q

L

G

L

C

A

L

W

R

R

A

L

G

A

Q

R

L

L

M

K

D

D

A

E

G

G

E

K

I

L

A

T

P

P

E

A

L

Q

I

V

S

S

L

L

I

A

K

K

R

R

N

Q

S

N

C

V

G

W

K

K

A

A

L

L

D

Q

I

A

A

A

R

R

T

M

A

A

R

R

D
|
D

M
x
I

L

L

G

G

G
|
G

N

S

G

G

I

I

S

T

D

L

E

E

Y

Y

P

H

I

A

M

I

R

R

H

H

M

M

V

L

N

N

L

L

E

E

V
x
T

V

V

N

Y

T
|
T

Y
|
Y

E
|
E

G

G

T
|
T

H

H

D

D

V

V

H

H

A

T

L

L

I

V

L

L

G

G

R
|
R

T

E

Q

I

T

T

D

G

I

L

P

N

A

A

F

F

active site: L131 (= L140), T132 (= T141), A239 (= A252), E360 (= E373), R372 (= R385)
binding flavin-adenine dinucleotide: L131 (= L140), T132 (= T141), G136 (≠ H145), G137 (= G146), S138 (= S147), W161 (= W171), T163 (= T173), R265 (= R278), L272 (= L285), K275 (= K288), D333 (= D346), I334 (≠ M347), G337 (= G350), T355 (≠ V368), T358 (= T371), Y359 (= Y372), T362 (= T375)

3swoA Crystal structure of a glutaryl-coa dehydrogenase from mycobacterium smegmatis in complex with fadh2 (see paper)
46% identity, 96% coverage: 15:393/393 of query aligns to 10:387/388 of 3swoA

query
sites
3swoA

F

F

L

D

L

T

E

D

H

R

Q

L

L

L

T

D

D

Q

E

D

Q

E

R

R

M

D

V

I

Q

A

S

T

A

V

R

R

Q

Q

Y

F

A

V

Q

D

D

T

K

R

L

L

A

K

P

P

R

N

V

V

R

E

E

G

A

W

F

F

R

E

A

S

E

A

H

T

T

L

D

P

R

S

A

E

I

L

F

A

N

K

E

E

M

F

G

G

E

N

L

L

G

G

L

V

L

L

G

G

S

M

T

H

I

L

D

Q

G

G

Y

Y

G

G

C

C

P

A

G

G

V

T

D

N

Y

A

I

V

S

S

Y

Y

G

G

L

L

V

A

A

C

R

M

E

E

V

L

E

E

R

A

V

G

D

D

S

S

G

G

Y

F

R

R

S

S

M

F

M

V

S

S

V

V

Q

Q

S

G

S

S

L

L

V

S

M

M

Y

F

P

S

I

I

W

Y

A

R

Y

Y

G

G

N

S

E

E

A

E

Q

Q

R

K

E

N

K

E

Y

W

L

L

P

P

K

R

L

L

A

A

T

A

G

G

E

D

W

A

V

I

G

G

C

C

F
|
F

G

G

L
|
L

T
|
T

E

E

P

P

D

D

H

F

G
|
G

S
|
S

D

N

P

P

G

A

S

G

M

M

V

R

T

T

R

R

A

A

R

R

S

R

V

D

E

G

G

S

G

D

Y

W

S

I

L

L

S

N

G

G

A

T

K

K

M

M

W
|
W

I
|
I

T
|
T

N

N

S

G

P

N

F

L

A

A

D

D

V

V

F

A

V

T

V

V

W

W

A

A

K

Q

T

T

D

D

G

G

K

-

I

I

R

R

G

G

F

F

V

L

L

V

E

P

K

T

G

D

M

T

E

P

G

G

L

F

S

T

A

A

P

N

K

E

I

I

E

H

G

R

K

K

L

L

A

S

L

L

R

R

A

A

S

S

V

V

T

T

G

S

E

E

I

L

V

V

M

L

D

D

E

N

V

V

F

R

V

L

P

P

E

A

E

S

N

A

L

Q

L

L

P

P

G

L

V

A

E

E

G

G

L

L

K

S

G

A

P

P

F

L

G

S

C

C

L

L

N

N

N

E

A
|
A

R

R

Y

F

G

G

I

I

A

V

W

F

G

G

A

A

I

L

G

G

A

A

E

R

T

D

C

S

W

L

H

E

A

T

R

I

E

A

Y

Y

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active site: L135 (= L140), T136 (= T141), A246 (= A252), E367 (= E373), K379 (≠ R385)
binding dihydroflavine-adenine dinucleotide: F133 (= F138), L135 (= L140), T136 (= T141), G141 (= G146), S142 (= S147), W166 (= W171), I167 (= I172), T168 (= T173), R272 (= R278), V274 (≠ Q280), F275 (= F281), L279 (= L285), Y282 (≠ K288), T340 (≠ D346), L341 (≠ M347), G344 (= G350), I347 (= I353), T365 (= T371), Y366 (= Y372), T369 (= T375), E371 (≠ D377), M372 (≠ V378)

Q96329 Acyl-coenzyme A oxidase 4, peroxisomal; AOX 4; G6p; Short-chain acyl-CoA oxidase; AtCX4; AtG6; SAOX; EC 1.3.3.6 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
37% identity, 97% coverage: 13:393/393 of query aligns to 47:428/436 of Q96329

query
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Q96329

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WALT 172:175 (≠ FGLT 138:141) binding FAD
S181 (= S147) binding FAD
SDAS 181:184 (≠ SDPG 147:150) binding an acyl-CoA
G207 (≠ T173) binding FAD
R288 (= R253) binding an acyl-CoA
RKQ 313:315 (= RKQ 278:280) binding FAD
Q324 (= Q289) binding FAD
E381 (≠ D346) binding FAD
G385 (= G350) binding FAD
G409 (= G374) binding an acyl-CoA
TYD 410:412 (≠ THD 375:377) binding FAD
F428 (= F393) binding FAD

2ix6A Short chain specific acyl-coa oxidase from arabidopsis thaliana, acx4 (see paper)
37% identity, 97% coverage: 12:393/393 of query aligns to 30:412/416 of 2ix6A

query
sites
2ix6A

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