SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_084199459.1 NCBI__GCF_002869505.1:WP_084199459.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P9WQ37 Long-chain-fatty-acid--CoA ligase FadD13; Fatty acyl-CoA ligase; FACL; FACL13; Fatty acyl-CoA synthetase; ACS; FACS; Very-long-chain fatty-acyl-CoA synthetase; ACSVL; EC 6.2.1.3 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 4 papers)
37% identity, 96% coverage: 16:507/511 of query aligns to 10:496/503 of P9WQ37

query
sites
P9WQ37

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R17 (≠ D23) mutation to A: Alteration of the strength of the membrane binding; when associated with A-9; A-17; A-197 and A-244.
K172 (= K179) mutation to A: Slight reduction of the fatty acyl-CoA ligase activity. Slight increase of susceptibility to proteolysis.
R195 (vs. gap) mutation to A: Alteration of the strength of the membrane binding; when associated with A-9; A-17; A-197 and A-244.
R197 (= R203) mutation to A: Alteration of the strength of the membrane binding; when associated with A-9; A-17; A-195 and A-244.
V209 (≠ I216) mutation to D: Strong reduction of the fatty acyl-CoA ligase activity. No significant change in the total expression level, however the cytoplasmic expression is reduced. Slight increase of susceptibility to proteolysis.
A211 (≠ G218) mutation to G: Slight increase of the fatty acyl-CoA ligase activity. Reduced rate of proteolytic degradation.
T214 (≠ V221) mutation to W: Shows a marked decrease in the activity with lauric and palmitic acid (C12 and C16 fatty acid) with a simultaneous increase in the activity with caprylic acid (C8 fatty acid).
R244 (≠ K252) mutation to A: Alteration of the strength of the membrane binding; when associated with A-17; A-195; A-195 and A-197.
A302 (≠ G310) mutation to G: Slight increase of the fatty acyl-CoA ligase activity. Reduced rate of proteolytic degradation.; mutation to W: Does not show activity with small, medium or long acyl chains.
W377 (= W387) mutation to A: Strong reduction of the fatty acyl-CoA ligase activity. Enhanced affinity towards palmitic acid binding. No significant change in the total expression level, however the cytoplasmic expression is low. Slight increase of susceptibility to proteolysis.
D382 (= D392) mutation to A: Strong reduction of the fatty acyl-CoA ligase activity. No significant change in the total expression level, however the cytoplasmic expression is reduced.
R397 (= R407) mutation to A: Reduction of binding affinity for fatty acids.
S404 (= S414) mutation to A: Slight reduction of the fatty acyl-CoA ligase activity. Enhanced affinity towards palmitic acid binding.
G406 (= G416) mutation to L: No effect on the formation of acyl-adenylate intermediate. However, it shows very poor catalytic efficiency to form acyl-CoA.
K487 (= K498) mutation to A: Strong reduction of the fatty acyl-CoA ligase activity. Reduction of binding affinity for ATP.

Sites not aligning to the query:

9 R→A: Alteration of the strength of the membrane binding; when associated with A-9; A-195; A-197 and A-244.

3r44A Mycobacterium tuberculosis fatty acyl coa synthetase (see paper)
37% identity, 96% coverage: 16:507/511 of query aligns to 13:496/502 of 3r44A

query
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active site: T167 (= T171), A184 (≠ S196), H208 (= H215), T304 (= T312), E305 (= E313), I403 (= I413), N408 (= N418), K487 (= K498)
binding histidine: E179 (≠ N191), H182 (≠ N194)

Sites not aligning to the query:

binding histidine: 5

5gtdA O-succinylbenzoate coa synthetase (mene) from bacillus subtilis in complex with the acyl-adenylate intermediate osb-amp (see paper)
35% identity, 97% coverage: 15:511/511 of query aligns to 9:483/484 of 5gtdA

query
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5gtdA

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S

S

G

G

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T

G

G

L

K

P

P

K

K

G

G

A

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M

Q

L

Q

T

T

H

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G

G

N

N

F

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Y

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S

N

A

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N

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A

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G

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H

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|
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active site: T151 (= T171), S171 (≠ N191), H195 (= H215), T288 (= T312), E289 (= E313)
binding adenosine-5'-monophosphate: G263 (≠ A287), G264 (≠ A288), Y285 (= Y309), G286 (= G310), M287 (≠ L311), T288 (= T312), D366 (= D392), V378 (≠ I404)
binding magnesium ion: F314 (≠ Y339), S315 (= S340)
binding 2-succinylbenzoate: H195 (= H215), S197 (≠ G217), A237 (≠ G258), L260 (≠ V284), G262 (= G286), G263 (≠ A287), G286 (= G310), M287 (≠ L311), S292 (≠ P316), Q293 (≠ F317)

5x8fB Ternary complex structure of a double mutant i454ra456k of o- succinylbenzoate coa synthetase (mene) from bacillus subtilis bound with amp and its product analogue osb-ncoa at 1.76 angstrom (see paper)
34% identity, 97% coverage: 15:511/511 of query aligns to 9:483/485 of 5x8fB

query
sites
5x8fB

L

M

R

Q

R

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A

A

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Y

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|
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|
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active site: T151 (= T171), S171 (≠ N191), H195 (= H215), T288 (= T312), E289 (= E313), I387 (= I413), N392 (= N418), K470 (= K498)
binding magnesium ion: Y23 (≠ F29), E24 (= E30), H70 (≠ F76), N178 (≠ A198), L202 (≠ M222), L214 (= L235), T296 (≠ L320), L297 (= L321), S298 (≠ T322)
binding o-succinylbenzoyl-N-coenzyme A: K85 (≠ R91), L191 (≠ A211), P192 (= P212), H195 (= H215), I196 (= I216), S197 (≠ G217), A237 (≠ G258), V238 (≠ A259), L260 (≠ V284), G262 (= G286), G286 (= G310), M287 (≠ L311), S292 (≠ P316), Q293 (≠ F317), S388 (= S414), G389 (= G415), G390 (= G416), E391 (= E417), K420 (= K446), W421 (= W447), K450 (≠ R478), Y451 (= Y479)

5busA O-succinylbenzoate coenzyme a synthetase (mene) from bacillus subtilis, in complex with amp (see paper)
35% identity, 97% coverage: 15:511/511 of query aligns to 8:480/481 of 5busA

query
sites
5busA

L

M

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G

Y

Y

L

L

D

D

D

N

E

E

G

G

F

F

L

L

Y

Y

I
x
V

C

L

D

D

R
|
R

L

R

K

S

D

D

M

L

V

I

I

I

S

S

G

G

E

E

N

N

V

I

Y

Y

P

P

A

A

E

E

V

V

E

E

G

S

V

V

L

L

Y

L

K

S

H

H

E

P

A

A

I

V

A

A

E

E

V

A

A

G

V

V

I

S

G

G

T

A

P

E

D

D

E

K

K

K

W

-

G

-

E

K

A

V

V

P

T

H

A

A

V

Y

V

L

A

V

L

L

H

H

E

K

G

P

A

V

S

S

L

A

T

G

L

-

E

-

G

E

L

L

R

T

E

D

F

Y

A

C

D

K

P

E

F

R

L

L

A

A

R

K

Y

Y

K

K

L

I

P

P

L

A

R

K

M

F

E

F

V

V

V

L

E

D

A

R

L

L

P

P

R

R

N

N

P

A

A

S

G

N

K
|
K

V

L

L

L

K

R

F

N

V

Q

L

L

K

K

E

D

Q

A

I

R

V

K

G

G

E

E

active site: T150 (= T171), S170 (≠ N191), H194 (= H215), T287 (= T312), E288 (= E313)
binding adenosine monophosphate: H194 (= H215), G262 (≠ A287), G263 (≠ A288), S283 (≠ G308), M286 (≠ L311), T287 (= T312), D365 (= D392), V377 (≠ I404), R380 (= R407), K467 (= K498)

5burA O-succinylbenzoate coenzyme a synthetase (mene) from bacillus subtilis, in complex with atp and magnesium ion (see paper)
35% identity, 96% coverage: 15:506/511 of query aligns to 8:475/475 of 5burA

query
sites
5burA

L

M

R

Q

R

R

A

A

D

Q

L

L

N

T

P

P

D

E

R

R

I

I

A

A

V

L

V

I

F

Y

E

E

E

D

Q

Q

E

T

I

V

T

T

Y

F

A

A

E

E

F

L

A

F

D

A

R

A

V

S

R

K

R

R

Q

M

A

A

Q

E

L

Q

L

L

R

A

D

A

A

H

G

S

V

V

C

R

V

K

G

G

D

D

R

T

V

A

G

A

Y

I

L

L

G

L

F

Q

N

N

H

R

P

A

A

E

L

M

L

V

E

Y

T

A

L

V

F

H

A

A

T

C

Q

F

A

L

L

L

G

G

A

V

I

K

F

A

V

V

P

L

L

L

N

N

F

T

R

K

L

L

T

S

A

T

E

H

E

E

L

R

T

L

F

F

I

Q

I

L

N

E

D

D

A

S

G

G

V

S

H

G

S

F

L

L

V

L

V

T

D

D

D

S

A

S

L

F

R

E

P

-

V

-

E

-

P

-

A

-

L

-

D

-

K

-

L

-

C

-

R

K

Y

E

F

Y

S

E

S

H

E

I

S

V

E

Q

A

T

D

I

G

D

W

V

R

D

H

E

L

L

S

M

S

K

E

E

R

A

A

A

A

E

C

E

E

I

P

E

L

I

T

E

S

A

T

Y

V

M

A

Q

V

M

D

D

Q

A

H

-

E

-

T

T

G

A

L

T

I

L

M

M

Y

Y

T
|
T

S
|
S

G

G

T
|
T

G

G

L

K

P

P

K
|
K

G

G

A

V

M

Q

L

Q

T

T

H

F

G

G

N

N

F

H

V

Y

W

F

N
x
S

N

A

I

V

N

S

A

S

S

A

M

L

A

N

F

L

G

G

A

I

S

T

R

E

D

Q

N

D

I

R

I

W

L

L

T

I

A

A

A

L

P

P

L

L

F

F

H
|
H

I

I

G

S

G

G

L

L

N

S

V

A

M

L

T

-

L

F

H

K

S

S

F

V

H

I

L

Y

G

G

S

M

K

T

L

V

V

V

L

L

L

H

R

Q

N

R

F

F

D

S

P

V

A

S

A

D

V

V

L

L

E

H

N

S

F

I

E

N

R

R

Y

H

K

E

V

V

T

T

H

M

M

I

F

S

G

A

A

V

P

Q

A

T

M

M

Y

-

L

-

F

-

M

L

A

A

Q

S

L

L

P

L

Q

E

F

E

A

T

D

N

T

R

D

C

L

P

S

E

S

S

L

I

D

R

V

C

L

I

V

L

C

L

G

G

A

G

A
x
G

P

P

V

A

P

P

E

L

S

P

L

L

I

L

E

E

T

E

Y

C

G

R

A

E

R

K

G

G

V

F

S

P

F

V

C

F

Q

Q

G
x
S

Y

Y

G

G

L

M

T
|
T

E
|
E

T

T

A

C

P

S

F

Q

S

I

A

V

L

T

L

L

T

S

P

P

E

E

H

F

A

S

V

M

S

E

K

K

L

L

G

G

S

S

A

A

G

G

Q

K

V

-

P

P

L

L

Y

F

S

S

G

C

T

E

R

I

I

K

V

I

D

E

A

R

D

D

N

G

Q

Q

P

V

L

C

P

E

A

P

G

Y

E

E

K

H

G

G

E

E

I

I

C

M

L

V

R

K

G

G

P

P

N

N

I

V

M

M

K

K

G

S

Y

Y

W

F

N

N

R

R

P

E

E

S

A

A

T

N

A

E

S

A

A

S

I

F

D

Q

S

N

E

-

G

G

W

W

F

L

H

K

S

T

G

G

D
|
D

I

L

G

G

Y

Y

L

L

D

D

D

N

E

E

G

G

F

F

L

L

Y

Y

I
x
V

C

L

D

D

R
|
R

L

R

K

S

D

D

M

L

V

I

I

I

S

S

G

G

E

E

N

N

V

I

Y

Y

P

P

A

A

E

E

V

V

E

E

G

S

V

V

L

L

Y

L

K

S

H

H

E

P

A

A

I

V

A

A

E

E

V

A

A

G

V

V

I

S

G

G

T

A

P

E

D

D

E

K

K

-

W

-

G

G

E

K

A

V

V

P

T

H

A

A

V

Y

V

L

A

V

L

L

H

H

E

K

G

P

A

V

S

S

L

A

T

G

L

-

E

-

G

E

L

L

R

T

E

D

F

Y

A

C

D

K

P

E

F

R

L

L

A

A

R

K

Y

Y

K

K

L

I

P

P

L

A

R

K

M

F

E

F

V

V

V

L

E

D

A

R

L

L

P

P

R

R

N

N

P

A

A

S

G

N

K

K

V

L

L

L

K

R

F

N

V

Q

L

L

K

K

E

D

active site: T150 (= T171), S170 (≠ N191), H194 (= H215), T287 (= T312), E288 (= E313)
binding adenosine-5'-triphosphate: T150 (= T171), S151 (= S172), T153 (= T174), T154 (= T175), K158 (= K179), G263 (≠ A288), S283 (≠ G308), T287 (= T312), D365 (= D392), V377 (≠ I404), R380 (= R407)

Q5SKN9 Long-chain-fatty-acid--CoA ligase; Long-chain fatty acyl-CoA synthetase; LC-FACS; EC 6.2.1.3 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
36% identity, 94% coverage: 30:507/511 of query aligns to 44:533/541 of Q5SKN9

query
sites
Q5SKN9

E

E

E

V

Q

H

E

R

I

T

T

T

Y

Y

A

A

E

E

F

V

A

Y

D

Q

R

R

V

A

R

R

Q

L

A

M

Q

G

L

G

L

L

R

R

D

A

A

L

G

G

V

V

C

G

V

V

G

G

D

D

R

R

V

V

G

A

Y

T

L

L

G

G

F

F

N

N

H

H

P

F

A

R

L

H

L

L

E

E

T

A

L

Y

F

F

A

A

T

V

Q

P

A

G

L

M

G

G

A

A

I

V

F

L

V

H

P

T

L

A

N

N

F

P

R

R

L

L

T

S

A

P

E

K

E

E

L

I

T

A

F

Y

I

I

I

L

N

N

D

H

A

A

G

E

V

D

H

K

S

V

L

L

V

L

V

F

D

D

D

P

A

N

L

L

R

L

P

P

V

L

V

V

E

E

P

A

A

I

L

R

D

G

K

E

L

L

C

K

C

T

R

V

R

Q

Y

H

F

F

S

V

E

M

S

D

E

E

A

K

D

A

G

P

W

E

R

G

H

Y

L

L

S

A

S

Y

E

E

R

E

A

A

A

L

C

G

E

E

P

E

L

-

T

A

S

D

T

P

V

V

A

R

V

V

D

P

Q

E

H

R

E

A

T

A

G

C

L

G

I

M

M

A

Y

Y

T
|
T

S

T

G

G

T

T

G

G

L

L

P

P

K

K

G

G

A

V

M

V

L

Y

T

S

H

H

G

R

N

A

F

L

V

V

W

L

N

H

N

S

I

L

N

A

A

A

S

S

M

L

A

V

F

D

G

G

A

T

-

A

-

L

S

S

R

E

D

K

N

D

I

V

L

L

T

P

A

V

P

P

L

M

F

F

H

H

I

V

G

N

G

A

L

W

N

-

V

C

M

L

T

P

L

Y

H

A

S

A

F

T

H

L

L

V

G

G

S

A

K

K

L

Q

V

V

L

L

L

P

-

G

R

P

N

R

F

L

D

D

P

P

A

A

A

S

V

L

L

V

E

E

N

L

F

F

E

D

R

G

Y

E

K

G

V

V

T

T

H

F

M

T

F

A

G

G

A

V

P

P

A

T

M

V

Y

W

L

L

F

A

M

L

A

A

Q

D

L

Y

P

L

Q

E

F

S

A

T

D

G

T

H

D

R

L

L

S

K

S

T

L

L

D

R

V

R

L

L

V

V

C

V

G

G

A
x
G

A

S

P

A

V

A

P

P

E

R

S

S

L

L

I

I

E

A

T

R

Y

F

G

E

A

R

R

M

G

G

V

V

S

E

F

V

C

R

Q
|
Q

G
|
G

Y

Y

G

G

L

L

T
|
T

E
|
E

T

T

A

S

P

P

-

V

-

Q

-

N

-

F

-

V

-

K

-

S

-

H

F

L

S

E

A

S

L

L

L

S

T

E

P

E

E

E

H

K

A

L

V

T

S

L

K

K

L

A

G

K

S

T

A

G

G

L

Q

P

V

I

P

P

L

L

Y

V

S

R

G

-

T

L

R

R

I

V

V

A

D

D

A

E

D

E

N

G

Q

R

P

P

L

V

P

P

A

K

G

D

E

G

K

K

-

A

-

L

G

G

E

E

I

V

C

Q

L

L

R

K

G

G

P

P

N

W

I

I

M

T

K

G

G

G

Y

Y

W

Y

N

G

R

N

P

E

E

E

A

A

T

T

A

R

S

S

A

A

I

L

D

T

S

P

E

D

G

G

W

F

F

F

H

R

S

T

G

G

D
|
D

I

I

G

A

Y

V

L

W

D

D

D

E

E

E

G

G

F

Y

L

V

Y

E

I

I

C

K

D

D

R

R

L

L

K
|
K

D

D

M

L

V

I

I
x
K

S

S

G

G

E

E

N

W

V

I

Y

S

P

S

A

V

E

D

V

L

E

E

G

N

V

A

L

L

Y

M

K

G

H

H

E

P

A

K

I

V

A

K

E

E

V

A

A

A

V

V

I

V

G

A

T

I

P

P

D

H

E

P

K

K

W

W

G

Q

E

E

A

R

V

P

T

L

A

A

V

V

A

-

L

V

H

P

E

R

G

G

A

E

S

K

L

P

T

T

L

P

E

E

G

E

L

L

R

N

E

E

-

H

F

L

A

L

D

K

P

A

F

G

L

F

A

A

R

K

Y

W

K

Q

L

L

P

P

L

D

R

A

M

Y

E

V

V

F

V

A

E

E

A

E

L

I

P

P

R

R

N

T

P

S

A

A

G

G

K

K

V

F

L

L

K

K

F

R

V

A

L

L

K

R

E

E

Q

Q

T184 (= T171) binding Mg(2+)
G302 (≠ A287) binding tetradecanoyl-AMP
Q322 (= Q307) binding tetradecanoyl-AMP
G323 (= G308) binding tetradecanoyl-AMP
T327 (= T312) binding tetradecanoyl-AMP
E328 (= E313) binding Mg(2+)
D418 (= D392) binding tetradecanoyl-AMP
K435 (= K409) binding tetradecanoyl-AMP
K439 (≠ I413) binding tetradecanoyl-AMP

5buqB Unliganded form of o-succinylbenzoate coenzyme a synthetase (mene) from bacillus subtilis, solved at 1.98 angstroms (see paper)
34% identity, 97% coverage: 15:511/511 of query aligns to 8:471/473 of 5buqB

query
sites
5buqB

L

M

R

Q

R

R

A

A

D

Q

L

L

N

T

P

P

D

E

R

R

I

I

A

A

V

L

V

I

F

Y

E

E

E

D

Q

Q

E

T

I

V

T

T

Y

F

A

A

E

E

F

L

A

F

D

A

R

A

V

S

R

K

R

R

Q

M

A

A

Q

E

L

Q

L

L

R

A

D

A

A

H

G

S

V

V

C

R

V

K

G

G

D

D

R

T

V

A

G

A

Y

I

L

L

G

L

F

Q

N

N

H

R

P

A

A

E

L

M

L

V

E

Y

T

A

L

V

F

H

A

A

T

C

Q

F

A

L

L

L

G

G

A

V

I

K

F

A

V

V

P

L

L

L

N

N

F

T

R

K

L

L

T

S

A

T

E

H

E

E

L

R

T

L

F

F

I

Q

I

L

N

E

D

D

A

S

G

G

V

S

H

G

S

F

L

L

V

L

V

T

D

D

D

S

A

S

L

F

R

E

P

-

V

-

E

-

P

-

A

-

L

-

D

-

K

-

L

-

C

-

R

K

Y

E

F

Y

S

E

S

H

E

I

S

V

E

Q

A

T

D

I

G

D

W

V

R

D

H

E

L

L

S

M

S

K

E

E

R

A

A

A

A

E

C

E

E

I

P

E

L

I

T

E

S

A

T

Y

V

M

A

Q

V

M

D

D

Q

A

H

-

E

-

T

T

G

A

L

T

I

L

M

M

Y

Y

T
|
T

S

S

G

K

T

-

G

-

L

-

P

P

K

K

G

G

A

V

M

Q

L

Q

T

T

H

F

G

G

N

N

F

H

V

Y

W

F

N
x
S

N

A

I

V

N

S

A

S

S

A

M

L

A

N

F

L

G

G

A

I

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R

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Q

N

D

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L

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I

A

A

A

L

P

P

L

L

F

F

H
|
H

I

I

G

S

G

G

L

L

N

S

V

A

M

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T

-

L

F

H

K

S

S

F

V

H

I

L

Y

G

G

S

M

K

T

L

V

V

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Q

N

R

F

F

D

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P

V

A

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A

D

V

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E

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N

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Y

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E

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V

T

T

H

M

M

I

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S

G

A

A

V

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Q

A

T

M

M

Y

-

L

-

F

-

M

L

A

A

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S

L

L

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L

Q

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F

E

A

T

D

N

T

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E

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S

L

I

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R

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C

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I

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A

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P

L

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I

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E

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T

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Y

C

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R

A

E

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K

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G

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Q

G

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Y

Y

G

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L

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T
|
T

E
|
E

T

T

A

C

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S

F

Q

S

I

A

V

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T

L

L

T

S

P

P

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A

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M

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E

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L

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A

A

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S

S

G

C

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Y

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K

H

G

G

E

E

I

I

C

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K

G

G

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P

N

N

I

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M

K

K

G

S

Y

Y

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F

N

N

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P

E

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A

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T

N

A

E

S

A

A

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I

F

D

Q

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E

-

G

G

W

W

F

L

H

K

S

T

G

G

D

D

I

L

G

G

Y

Y

L

L

D

D

D

N

E

E

G

G

F

F

L

L

Y

Y

I

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C

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D

R

-

L

-

K

-

D

D

M

L

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I

I
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I

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E

E

N
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N

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Y

P

P

A

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x
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H

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x
V

A

A

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E

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A

A

G

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V

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G

T

A

P

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D

D

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-

K

-

W

-

G

-

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K

A

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V

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T

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A

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Y

V

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A

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L

L

H

H

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G

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S

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A

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G

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-

E

-

G

E

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L

R

T

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D

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Y

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P

E

F

R

L

L

A

A

R

K

Y

Y

K

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I

P

P

L

A

R

K

M

F

E

F

V

V

V

L

E

D

A

R

L

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R

N

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P

A

A

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K
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active site: T150 (= T171), S166 (≠ N191), H190 (= H215), T283 (= T312), E284 (= E313), I379 (= I413), N384 (= N418), K458 (= K498)
binding calcium ion: L395 (≠ Y429), H397 (= H431), V400 (≠ I434)

Q9S725 4-coumarate--CoA ligase 2; 4CL 2; 4-coumarate--CoA ligase isoform 2; At4CL2; 4-coumaroyl-CoA synthase 2; Caffeate--CoA ligase; EC 6.2.1.12; EC 6.2.1.- from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
31% identity, 93% coverage: 35:508/511 of query aligns to 65:550/556 of Q9S725

query
sites
Q9S725

T

T

Y

Y

A

A

E

D

F

V

A

H

D

V

R

T

V

S

R

R

R

K

Q

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A

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D

N

A

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Q

G

H

D

D

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V

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M

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L

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N

N

H

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P

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A

E

L

V

E

L

T

T

L

F

F

L

A

A

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A

Q

S

A

F

L

I

G

G

A

A

I

I

F

T

V

T

P

S

L

A

N

N

F

P

R

F

L

F

T

T

A

P

E

A

E

E

L

I

T

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F

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I

Q

I

A

N

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D

A

A

S

G

A

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A

H

K

S

L

L

I

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V

V

T

D

Q

D

S

A

R

L

Y

R

V

P

D

V

K

V

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E

K

P

N

A

L

L

Q

D

N

K

D

L

G

C

V

C

L

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I

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V

Y

T

F

T

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D

S

S

E

D

S

A

E

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A

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D

E

G

N

W

C

R

L

H

R

L

F

S

S

S

E

E

L

R

T

A

Q

A

S

C

E

E

E

P

P

L

R

T

V

S

D

T

S

V

I

-

P

-

E

A

K

V

I

D

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Q

P

H

E

E

D

T

V

G

V

L

A

I

L

M

P

Y

F

T

S

S

S

G

G

T

T

G

G

L

L

P

P

K
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K

G

G

A

V

M

M

L

L

T

T

H

H

G

K

N

G

F

L

V

V

W

T

-

S

-

V

-

A

-

Q

-

V

N

D

N

G

I

E

N

N

A

P

S

N

M

L

A

Y

F

F

G

-

A

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D

N

D

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I

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C

A

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A

L

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F

F

H

H

I

I

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Y

G

A

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N

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M

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A

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N

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M

A

F
x
M

G

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A

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P

A

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M

I

Y

V

L

L

F

A

M

I

A

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P

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A

E

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K

T

Y

D

D

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S

L

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x
K

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G

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A

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I

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E

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Y

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A

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Y

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M

T

T

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E

T

A

A

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A

A

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-

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A

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E

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G

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D

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K

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G

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E
|
E

I

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C

L

I

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R

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G

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N

Q

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I

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M

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K

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A

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D

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D

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|
R

L

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K

K

D

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x
K

G

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N

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A

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E

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L

L

L

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E

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I

A

N

E

D

V

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A

A

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D

E

E

E

K

D

W

A

G

G

E

E

A

V

V

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V

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A

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F

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V

A

V

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R

H

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E

K

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D

A

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N

L

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T

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L

E

E

D

G

E

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K

E

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F

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K

F

Q

L

V

A

V

R

F

Y

Y

K

K

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I

L

N

R

K

M

V

E

F

V

F

V

T

E

D

A

S

L

I

P

P

R

K

N

A

P

P

A

S

G

G

K
|
K

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F

K

V

D

L

L

K

R

E

A

Q

R

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L

K211 (= K179) mutation to S: Drastically reduces the activity.
M293 (≠ F257) mutation M->A,P: Affects the substrate specificity.
K320 (≠ V284) mutation K->L,A: Affects the substrate specificity.
E401 (= E359) mutation to Q: Slighlty reduces the substrate specificity.
C403 (= C361) mutation to A: Significantly reduces the substrate specificity.
R449 (= R407) mutation to Q: Drastically reduces the activity.
K457 (≠ G415) mutation to S: Drastically reduces the activity.
K540 (= K498) mutation to N: Abolishes the activity.

Q67W82 4-coumarate--CoA ligase 4; 4CL 4; Os4CL4; (E)-ferulate--CoA ligase; 4-coumaroyl-CoA synthase 4; Protein RESISTANCE TO ALUMINUM 1; EC 6.2.1.12; EC 6.2.1.34 from Oryza sativa subsp. japonica (Rice) (see paper)
33% identity, 93% coverage: 35:508/511 of query aligns to 61:545/559 of Q67W82

query
sites
Q67W82

T

S

Y

Y

A

G

E

E

F

V

A

E

D

E

R

L

V

S

R

R

Q

A

A

A

Q

A

L

G

L

L

R

R

D

R

A

L

G

G

V

V

C

G

V

K

G

G

D

D

R

V

V

V

G

M

Y

S

L

L

G

L