SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_084199684.1 NCBI__GCF_002869505.1:WP_084199684.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P0ABH7 Citrate synthase; EC 2.3.3.16 from Escherichia coli (strain K12) (see 2 papers)
69% identity, 99% coverage: 1:423/429 of query aligns to 1:421/427 of P0ABH7

query
sites
P0ABH7

M

M

S

A

D

D

K

T

K

K

A

A

T

K

L

L

T

T

I

L

D

N

G

G

M

-

D

D

E

T

A

A

V

V

E

E

L

L

P

D

I

V

Y

L

S

K

G

G

T

T

I

L

G

G

P

Q

D

D

V

V

V

I

D

D

V

I

G

R

S

T

L

L

T

G

G

S

K

K

G

G

M

V

F

F

T

T

Y

F

D

D

P

P

G

G

F

F

V

T

S

S

T

T

A

A

S

S

C

C

E

E

S

S

Q

K

I

I

T

T

Y

F

I

I

D

D

G

G

G

D

K

E

G

G

V

I

L

L

L

L

H

H

R

R

G

G

Y

F

P

P

I

I

E

D

Q

Q

L

L

A

A

E

T

H

D

S

S

D

N

Y

Y

L

L

E

E

T

V

C

C

Y

Y

L

I

L

L

L

L

Y

N

G

G

E

E

L

K

P

P

T

T

K

Q

E

E

Q

Q

K

Y

D

D

K

E

F

F

V

K

S

T

T

T

V

V

T

T

N

R

H

H

T

T

M

M

V

I

H

H

E

E

Q

Q

L

I

A

T

H

R

F

L

F

F

N

H

G

A

F

F

R

R

R

R

D

D

S

S

H

H

P

P

M

M

A

A

I

V

M

M

C

C

G

G

V

I

V

T

G

G

A

A

L

L

S

A

A

A

F

F

Y

Y

H

H

D

D

S

S

L

L

D

D

I

V

S

N

D

N

E

P

Q

R

H

H

R

R

Q

E

V

I

A

A

A

A

F

F

R

R

L

L

I

L

A

S

K

K

M

M

P

P

T

T

L

M

A

A

A

A

M

M

S

C

Y

Y

K

K

F

Y

S

S

I

I

G

G

Q

Q

P

P

F

F

M

V

Y

Y

P

P

D

R

N

N

S

D

M

L

G

S

Y

Y

A

A

E

G

N

N

F

F

L

L

H

N

M

M

M

M

F

F

G

S

V

T

P

P

C
|
C

E
|
E

K

P

S

Y

N

E

I

V

S

N

P

P

V

I

L

L

A

E

D

R

A

A

M

M

D

D

K

R

I

I

F

L

L

I

L

L

H

H

A

A

D

D

H

H

E

E

Q

Q

N

N

A

A

S

S

T

T

S

S

T

T

V

V

R

R

L

T

A

A

G

G

S

S

S

S

G

G

A

A

N

N

P

P

F

F

A

A

C

C

I

I

A

A

A

A

G

G

I

I

A

A

A

S

L

L

W

W

G

G

P

P

S

A

H

H

G

G

G

G

A

A

N

N

E

E

A

A

V

A

L

L

D

K

M

M

L

L

Q

E

E

E

I

I

G

S

D

S

V

V

S
x
K

R

H

I

I

D

P

E

E

F

F

V

V

A

R

R

R

A

A

K

K

D

D

K

K

N

N

D

D

P

S

F

F

R

R

L

L

M

M

G

G

F

F

G

G

H

H

R

R

V

V

Y

Y

K

K

N

N

F

Y

D

D

P

P

R

R

A

A

K

T

V

V

M

M

K

R

K

E

A

T

A

C

D

H

E

E

V

V

L

L

A

K

E

E

L

L

G

G

L

T

E

K

N

D

D

D

P

-

L

L

L

L

A

E

I

V

A

A

K

M

R

E

L

L

E

E

E

N

I

I

A

A

L

L

E

N

D

D

E

P

Y

Y

F

F

I

I

E

E

K

K

K

K

L

L

Y

Y

P

P

N

N

V

V

D

D

F

F

Y

Y

S

S

G

G

I

I

I

I

L

L

K

K

A

A

I

M

G

G

I

I

P

P

T

S

S

S

M

M

F

F

T

T

V

V

I

I

F

F

A

A

V

M

G

A

R

R

T

T

I

V

G

G

W

W

I

I

A

A

H

H

W

W

H

S

E

E

M

M

I

H

S

S

G

D

S

G

Y

M

R

K

I

I

G

A

R

R

P

P

R

R

Q

Q

L

L

Y

Y

T

T

G

G

H

Y

D

E

K

K

R

R

D

D

F

F

C207 (= C208) mutation to S: Weakened NADH binding and inhibition.
E208 (= E209) mutation to A: Weakened NADH binding and inhibition.
K283 (≠ S284) modified: N6-acetyllysine

4jagA Structural determination of the a50t:s279g:s280k:v281k:k282e:h283n variant of citrate synthase from e. Coli complexed with oxaloacetate (see paper)
69% identity, 98% coverage: 2:423/429 of query aligns to 1:420/426 of 4jagA

query
sites
4jagA

S

A

D

D

K

T

K

K

A

A

T

K

L

L

T

T

I

L

D

D

G

G

M

-

D

D

E

T

A

A

V

V

E

E

L

L

P

D

I

V

Y

L

S

K

G

G

T

T

I

L

G

G

P

Q

D

D

V

V

V

I

D

D

V

I

G

R

S

T

L

L

T

G

G

S

K

K

G

G

M

V

F

F

T

T

Y

F

D

D

P

P

G

G

F

F

V

T

S

S

T

T

A

T

S

S

C

C

E

E

S

S

Q

K

I

I

T

T

Y

F

I

I

D

D

G

G

G

D

K

E

G

G

V

I

L

L

L

L

H

H

R

R

G

G

Y

F

P

P

I

I

E

D

Q

Q

L

L

A

A

E

T

H

D

S

S

D

N

Y

Y

L

L

E

E

T

V

C

C

Y

Y

L

I

L

L

L

L

Y

N

G

G

E

E

L

K

P

P

T

T

K

Q

E

E

Q

Q

K

Y

D

D

K

E

F

F

V

K

S

T

T

T

V

V

T

T

N

R

H

H

T

T

M

M

V

I

H

H

E

E

Q

Q

L

I

A

T

H

R

F

L

F

F

N

H

G

A

F

F

R

R

R

R

D

D

S

S

H

H

P

P

M

M

A

A

I

V

M

M

C

C

G

G

V

I

V

T

G

G

A

A

L

L

S

A

A

A

F

F

Y

Y

H

H

D

D

S

S

L

L

D

D

I

V

S

N

D

N

E

P

Q

R

H

H

R

R

Q

E

V

I

A

A

A

A

F

F

R

R

L

L

I

L

A

S

K

K

M

M

P

P

T

T

L

M

A

A

A

A

M

M

S

C

Y

Y

K

K

F

Y

S

S

I

I

G

G

Q

Q

P

P

F

F

M

V

Y

Y

P

P

D

R

N

N

S

D

M

L

G

S

Y

Y

A

A

E

G

N

N

F

F

L

L

H

N

M

M

M

M

F

F

G

S

V

T

P

P

C

C

E

E

K

P

S

Y

N

E

I

V

S

N

P

P

V

I

L

L

A

E

D

R

A

A

M

M

D

D

K

R

I

I

F

L

L

I

L

L

H

H

A

A

D

D

H
|
H

E

E

Q

Q

N
|
N

A

A

S

S

T

T

S

S

T

T

V

V

R

R

L

T

A

A

G

G

S

S

S

S

G

G

A

A

N

N

P

P

F

F

A

A

C

C

I

I

A

A

A

A

G

G

I

I

A

A

A

S

L

L

W

W

G

G

P

P

S

A

H

H

G

G

G

G

A

A

N

N

E

E

A

A

V

A

L

L

D

K

M

M

L

L

Q

E

E

E

I

I

G

G

D

K

V

K

S

E

R

N

I

I

D

P

E

E

F

F

V

V

A

R

R

R

A

A

K

K

D

D

K

K

N

N

D

D

P

S

F

F

R

R

L

L

M

M

G

G

F

F

G

G

H

H

R
|
R

V

V

Y

Y

K

K

N

N

F

Y

D

D

P

P

R

R

A

A

K

T

V

V

M

M

K

R

K

E

A

T

A

C

D

H

E

E

V

V

L

L

A

K

E

E

L

L

G

G

L

T

E

K

N

D

D

D

P

-

L

L

L

L

A

E

I

V

A

A

K

M

R

E

L

L

E

E

E

N

I

I

A

A

L

L

E

N

D

D

E

P

Y

Y

F

F

I

I

E

E

K

K

K

K

L

L

Y

Y

P

P

N

N

V

V

D
|
D

F

F

Y

Y

S

S

G

G

I

I

I

I

L

L

K

K

A

A

I

M

G

G

I

I

P

P

T

S

S

S

M

M

F

F

T

T

V

V

I

I

F

F

A

A

V

M

G

A

R
|
R

T

T

I

V

G

G

W

W

I

I

A

A

H

H

W

W

H

S

E

E

M

M

I

H

S

S

G

D

S

G

Y

M

R

K

I

I

G

A

R

R

P

P

R

R

Q

Q

L

L

Y

Y

T

T

G

G

H

Y

D

E

K

K

R

R

D

D

F

F

active site: D362 (= D365)
binding oxaloacetate ion: H229 (= H231), N232 (= N234), R306 (= R308), R387 (= R390)

4jaeA Structural determination of the a50t:s279g:s280k:v281k:k282e:h283n variant of citrate synthase from e. Coli complexed with s- carboxymethyl-coa (see paper)
69% identity, 98% coverage: 2:423/429 of query aligns to 1:420/426 of 4jaeA

query
sites
4jaeA

S

A

D

D

K

T

K

K

A

A

T

K

L

L

T

T

I

L

D

D

G

G

M

-

D

D

E

T

A

A

V

V

E

E

L

L

P

D

I

V

Y

L

S

K

G

G

T

T

I

L

G

G

P

Q

D

D

V

V

V

I

D

D

V

I

G

R

S

T

L

L

T

G

G

S

K

K

G

G

M

V

F

F

T

T

Y

F

D

D

P

P

G

G

F

F

V

T

S

S

T

T

A

T

S

S

C

C

E

E

S

S

Q

K

I

I

T

T

Y

F

I

I

D

D

G

G

G

D

K

E

G

G

V

I

L

L

L

L

H

H

R

R

G

G

Y

F

P

P

I

I

E

D

Q

Q

L

L

A

A

E

T

H

D

S

S

D

N

Y

Y

L

L

E

E

T

V

C

C

Y

Y

L

I

L

L

L

L

Y

N

G

G

E

E

L

K

P

P

T

T

K

Q

E

E

Q

Q

K

Y

D

D

K

E

F

F

V

K

S

T

T
|
T

V

V

T
|
T

N
x
R

H
|
H

T
|
T

M
|
M

V

I

H

H

E

E

Q

Q

L

I

A

T

H

R

F

L

F

F

N

H

G

A

F

F

R

R

R

R

D

D

S

S

H

H

P

P

M

M

A

A

I

V

M

M

C

C

G

G

V

I

V

T

G

G

A

A

L

L

S

A

A

A

F

F

Y

Y

H

H

D

D

S

S

L

L

D

D

I

V

S

N

D

N

E

P

Q

R

H

H

R

R

Q
x
E

V
x
I

A

A

A

A

F

F

R
|
R

L

L

I

L

A

S

K
|
K

M

M

P

P

T

T

L

M

A

A

A

A

M

M

S

C

Y

Y

K

K

F

Y

S

S

I

I

G

G

Q

Q

P

P

F

F

M

V

Y

Y

P

P

D

R

N
|
N

S

D

M

L

G

S

Y

Y

A

A

E

G

N

N

F

F

L

L

H

N

M

M

M

M

F

F

G

S

V

T

P

P

C

C

E

E

K

P

S

Y

N

E

I

V

S

N

P

P

V

I

L

L

A

E

D

R

A

A

M

M

D

D

K

R

I

I

F

L

L

I

L

L

H

H

A

A

D

D

H

H

E

E

Q

Q

N

N

A

A

S

S

T

T

S

S

T

T

V

V

R

R

L

T

A

A

G

G

S

S

S

S

G

G

A

A

N

N

P

P

F

F

A

A

C

C

I

I

A

A

A

A

G

G

I

I

A

A

A

S

L

L

W

W

G

G

P

P

S

A

H

H

G

G

G

G

A

A

N

N

E

E

A

A

V

A

L

L

D

K

M

M

L

L

Q

E

E

E

I

I

G

G

D

K

V

K

S

E

R

N

I

I

D

P

E

E

F

F

V

V

A

R

R

R

A

A

K

K

D

D

K

K

N

N

D

D

P

S

F

F

R

R

L

L

M

M

G

G

F

F

G

G

H

H

R

R

V

V

Y

Y

K

K

N

N

F

Y

D

D

P

P

R

R

A

A

K

T

V

V

M

M

K

R

K

E

A

T

A

C

D

H

E

E

V

V

L

L

A

K

E

E

L

L

G

G

L

T

E

K

N

D

D

D

P

-

L

L

L

L

A

E

I

V

A

A

K

M

R

E

L

L

E

E

E

N

I

I

A

A

L

L

E

N

D

D

E

P

Y

Y

F

F

I

I

E

E

K

K

K

K

L

L

Y

Y

P

P

N

N

V

V

D
|
D

F

F

Y

Y

S

S

G

G

I

I

I

I

L

L

K

K

A

A

I

M

G

G

I

I

P

P

T

S

S

S

M

M

F

F

T

T

V

V

I

I

F

F

A

A

V

M

G

A

R

R

T

T

I

V

G

G

W

W

I

I

A

A

H

H

W

W

H

S

E

E

M

M

I

H

S

S

G

D

S

G

Y

M

R

K

I

I

G

A

R

R

P

P

R

R

Q

Q

L

L

Y

Y

T

T

G

G

H

Y

D

E

K

K

R

R

D

D

F

F

active site: D362 (= D365)
binding carboxymethyl coenzyme *a: T106 (= T108), T108 (= T110), R109 (≠ N111), H110 (= H112), T111 (= T113), M112 (= M114), E158 (≠ Q160), I159 (≠ V161), R163 (= R165), K167 (= K169), N189 (= N191)

1owbA Three dimensional structure analysis of the variant r109l nadh complex of type ii citrate synthase from e. Coli (see paper)
69% identity, 98% coverage: 2:423/429 of query aligns to 1:420/426 of 1owbA

query
sites
1owbA

S

A

D

D

K

T

K

K

A

A

T

K

L

L

T

T

I

L

D

N

G

G

M

-

D

D

E

T

A

A

V

V

E

E

L

L

P

D

I

V

Y

L

S

K

G

G

T

T

I

L

G

G

P

Q

D

D

V

V

V

I

D

D

V

I

G

R

S

T

L

L

T

G

G

S

K

K

G

G

M

V

F

F

T

T

Y

F

D

D

P

P

G

G

F

F

V

T

S

S

T

T

A

A

S

S

C

C

E

E

S

S

Q

K

I

I

T

T

Y

F

I

I

D

D

G

G

G

D

K

E

G

G

V

I

L

L

L

L

H

H

R

R

G

G

Y

F

P

P

I

I

E

D

Q

Q

L

L

A

A

E

T

H

D

S

S

D

N

Y

Y

L

L

E

E

T

V

C

C

Y

Y

L

I

L

L

L

L

Y

N

G

G

E

E

L

K

P

P

T

T

K

Q

E

E

Q

Q

K

Y

D

D

K

E

F

F

V

K

S

T

T

T

V

V

T
|
T

N
x
L

H
|
H

T
|
T

M
|
M

V

I

H
|
H

E

E

Q

Q

L

I

A

T

H

R

F

L

F

F

N

H

G

A

F

F

R

R

R

R

D

D

S

S

H

H

P

P

M

M

A

A

I

V

M

M

C

C

G

G

V

I

V

T

G

G

A

A

L

L

S

A

A

A

F

F

Y
|
Y

H

H

D

D

S

S

L

L

D

D

I

V

S

N

D

N

E

P

Q

R

H

H

R

R

Q

E

V
x
I

A

A

A

A

F
|
F

R
|
R

L

L

I

L

A

S

K
|
K

M

M

P

P

T

T

L

M

A

A

A

A

M

M

S

C

Y

Y

K

K

F

Y

S

S

I

I

G

G

Q

Q

P

P

F

F

M

V

Y

Y

P

P

D

R

N
|
N

S

D

M

L

G

S

Y

Y

A

A

E

G

N

N

F

F

L

L

H

N

M

M

M

M

F

F

G

S

V

T

P

P

C

C

E

E

K

P

S

Y

N

E

I

V

S

N

P

P

V

I

L

L

A

E

D

R

A

A

M

M

D

D

K

R

I

I

F

L

L

I

L

L

H

H

A

A

D

D

H

H

E

E

Q

Q

N

N

A

A

S

S

T

T

S

S

T

T

V

V

R

R

L

T

A

A

G

G

S

S

S

S

G

G

A

A

N

N

P

P

F

F

A

A

C

C

I

I

A

A

A

A

G

G

I

I

A

A

A

S

L

L

W

W

G

G

P

P

S

A

H

H

G

G

G

G

A

A

N

N

E

E

A

A

V

A

L

L

D

K

M

M

L

L

Q

E

E

E

I

I

G

S

D

S

V

V

S

K

R

H

I

I

D

P

E

E

F

F

V

F

A

R

R

R

A

A

K

K

D

D

K

K

N

N

D

D

P

S

F

F

R

R

L

L

M

M

G

G

F

F

G

G

H

H

R

R

V

V

Y

Y

K

K

N

N

F

Y

D

D

P

P

R

R

A

A

K

T

V

V

M

M

K

R

K

E

A

T

A

C

D

H

E

E

V

V

L

L

A

K

E

E

L

L

G

G

L

T

E

K

N

D

D

D

P

-

L

L

L

L

A

E

I

V

A

A

K

M

R

E

L

L

E

E

E

N

I

I

A

A

L

L

E

N

D

D

E

P

Y

Y

F

F

I

I

E

E

K

K

K

K

L

L

Y

Y

P

P

N

N

V

V

D
|
D

F

F

Y

Y

S

S

G

G

I

I

I

I

L

L

K

K

A

A

I

M

G

G

I

I

P

P

T

S

S

S

M

M

F

F

T

T

V

V

I

I

F

F

A

A

V

M

G

A

R

R

T

T

I

V

G

G

W

W

I

I

A

A

H

H

W

W

H

S

E

E

M

M

I

H

S

S

G

D

S

G

Y

M

R

K

I

I

G

A

R

R

P

P

R

R

Q

Q

L

L

Y

Y

T

T

G

G

H

Y

D

E

K

K

R

R

D

D

F

F

active site: D362 (= D365)
binding nicotinamide-adenine-dinucleotide: T108 (= T110), L109 (≠ N111), H110 (= H112), T111 (= T113), M112 (= M114), H114 (= H116), Y145 (= Y147), I159 (≠ V161), F162 (= F164), R163 (= R165), K167 (= K169), N189 (= N191)

1nxgA The f383a variant of type ii citrate synthase complexed with nadh (see paper)
68% identity, 98% coverage: 2:423/429 of query aligns to 1:420/426 of 1nxgA

query
sites
1nxgA

S

A

D

D

K

T

K

K

A

A

T

K

L

L

T

T

I

L

D

N

G

G

M

-

D

D

E

T

A

A

V

V

E

E

L

L

P

D

I

V

Y

L

S

K

G

G

T

T

I

L

G

G

P

Q

D

D

V

V

V

I

D

D

V

I

G

R

S

T

L

L

T

G

G

S

K

K

G

G

M

V

F

F

T

T

Y

F

D

D

P

P

G

G

F

F

V

T

S

S

T

T

A

A

S

S

C

C

E

E

S

S

Q

K

I

I

T

T

Y

F

I

I

D

D

G

G

G

D

K

E

G

G

V

I

L

L

L

L

H

H

R

R

G

G

Y

F

P

P

I

I

E

D

Q

Q

L

L

A

A

E

T

H

D

S

S

D

N

Y

Y

L

L

E

E

T

V

C

C

Y

Y

L

I

L

L

L

L

Y

N

G

G

E

E

L

K

P

P

T

T

K

Q

E

E

Q

Q

K

Y

D

D

K

E

F

F

V

K

S

T

T
|
T

V

V

T

T

N
x
R

H
|
H

T
|
T

M
|
M

V
x
I

H
|
H

E

E

Q

Q

L

I

A

T

H

R

F

L

F

F

N

H

G

A

F

F

R

R

R

R

D

D

S

S

H

H

P

P

M

M

A

A

I

V

M

M

C

C

G

G

V

I

V

T

G

G

A

A

L

L

S

A

A

A

F

F

Y
|
Y

H

H

D

D

S

S

L

L

D

D

I

V

S

N

D

N

E

P

Q

R

H

H

R

R

Q

E

V

I

A

A

A

A

F
|
F

R
|
R

L

L

I

L

A

S

K
|
K

M

M

P

P

T

T

L

M

A

A

A

A

M

M

S

C

Y

Y

K

K

F

Y

S

S

I

I

G

G

Q

Q

P

P

F

F

M

V

Y

Y

P

P

D

R

N

N

S

D

M

L

G

S

Y

Y

A

A

E

G

N

N

F

F

L

L

H

N

M

M

M

M

F

F

G

S

V

T

P

P

C

C

E

E

K

P

S

Y

N

E

I

V

S

N

P

P

V

I

L

L

A

E

D

R

A

A

M

M

D

D

K

R

I

I

F

L

L

I

L

L

H

H

A

A

D

D

H

H

E

E

Q

Q

N

N

A

A

S

S

T

T

S

S

T

T

V

V

R

R

L

T

A

A

G

G

S

S

S

S

G

G

A

A

N

N

P

P

F

F

A

A

C

C

I

I

A

A

A

A

G

G

I

I

A

A

A

S

L

L

W

W

G

G

P

P

S

A

H

H

G

G

G

G

A

A

N

N

E

E

A

A

V

A

L

L

D

K

M

M

L

L

Q

E

E

E

I

I

G

S

D

S

V

V

S

K

R

H

I

I

D

P

E

E

F

F

V

F

A

R

R

R

A

A

K

K

D

D

K

K

N

N

D

D

P

S

F

F

R

R

L

L

M

M

G

G

F

F

G

G

H

H

R

R

V

V

Y

Y

K

K

N

N

F

Y

D

D

P

P

R

R

A

A

K

T

V

V

M

M

K

R

K

E

A

T

A

C

D

H

E

E

V

V

L

L

A

K

E

E

L

L

G

G

L

T

E

K

N

D

D

D

P

-

L

L

L

L

A

E

I

V

A

A

K

M

R

E

L

L

E

E

E

N

I

I

A

A

L

L

E

N

D

D

E

P

Y

Y

F

F

I

I

E

E

K

K

K

K

L

L

Y

Y

P

P

N

N

V

V

D
|
D

F

F

Y

Y

S

S

G

G

I

I

I

I

L

L

K

K

A

A

I

M

G

G

I

I

P

P

T

S

S

S

M

M

F

F

T

T

V

V

I

I

F

A

A

A

V

M

G

A

R

R

T

T

I

V

G

G

W

W

I

I

A

A

H

H

W

W

H

S

E

E

M

M

I

H

S

S

G

D

S

G

Y

M

R

K

I

I

G

A

R

R

P

P

R

R

Q

Q

L

L

Y

Y

T

T

G

G

H

Y

D

E

K

K

R

R

D

D

F

F

active site: D362 (= D365)
binding 1,4-dihydronicotinamide adenine dinucleotide: T106 (= T108), R109 (≠ N111), H110 (= H112), T111 (= T113), M112 (= M114), I113 (≠ V115), H114 (= H116), Y145 (= Y147), F162 (= F164), R163 (= R165), K167 (= K169)

2h12B Structure of acetobacter aceti citrate synthase complexed with oxaloacetate and carboxymethyldethia coenzyme a (cmx) (see paper)
64% identity, 99% coverage: 6:429/429 of query aligns to 3:426/426 of 2h12B

query
sites
2h12B

A

A

T

T

L

I

T

S

I

V

D

D

G

G

M

-

D

-

E

K

A

S

V

A

E

E

L

M

P

P

I

V

Y

L

S

S

G

G

T

T

I

L

G

G

P

P

D

D

V

V

V

I

D

D

V

I

G

R

S

K

L

L

T

P

G

A

K

Q

-

L

G

G

M

V

F

F

T

T

Y

F

D

D

P

P

G

G

F

Y

V

G

S

E

T

T

A

A

S

A

C

C

E

N

S

S

Q

K

I

I

T

T

Y

F

I

I

D

D

G

G

G

D

K

K

G

G

V

V

L

L

L

L

H

H

R

R

G

G

Y

Y

P

P

I

I

E

A

Q

Q

L

L

A

A

E

E

H

N

S

A

D

S

Y

Y

L

E

E

E

T

V

C

I

Y

Y

L

L

L

L

L

L

Y

N

G

G

E

E

L

L

P

P

T

N

K

K

E

A

Q

Q

K

Y

D

D

K

T

F

F

V

T

S

N

T

T

V

L

T

T

N

N

H

H

T

T

M

L

V

L

H

H

E

E

Q

Q

L

I

A

R

H

N

F

F

F

F

N

N

G

G

F

F

R

R

R

R

D

D

S

A

H

H

P

P

M

M

A

A

I

I

M

L

C

C

G

G

V

T

V

V

G

G

A

A

L

L

S

S

A

A

F

F

Y

Y

H

P

D

D

S

A

L

N

D

D

I

I

S

A

D

I

E

P

Q

A

H

N

R

R

Q

D

V

L

A

A

A

A

F

M

R

R

L

L

I

I

A

A

K

K

M

I

P

P

T

T

L

I

A

A

A

A

M

W

S

A

Y

Y

K

K

F

Y

S

T

I

Q

G

G

Q

E

P

A

F

F

M

I

Y

Y

P

P

D

R

N

N

S

D

M

L

G

N

Y

Y

A

A

E

E

N

N

F

F

L

L

H

S

M

M

M

M

F

F

G

A

V

R

P

M

C

S

E

E

K

P

S

Y

N

K

I

V

S

N

P

P

V

V

L

L

A

A

D

R

A

A

M

M

D

N

K

R

I

I

F

L

L

I

L

L

H

H

A

A

D

D

H
|
H

E

E

Q

Q

N
|
N

A

A

S
|
S

T

T

S

S

T

T

V

V

R

R

L

L

A

A

G

G

S

S

S

T

G

G

A

A

N

N

P

P

F

F

A

A

C

C

I

I

A

A

A

A

G

G

I

I

A

A

A

A

L

L

W

W

G

G

P

P

S
x
A

H
|
H

G

G

G

G

A
|
A

N

N

E

E

A

A

V

V

L

L

D

K

M

M

L

L

Q

A

E

R

I

I

G

G

D

K

V

K

S

E

R

N

I

I

D

P

E

A

F

F

V

I

A

A

R

Q

A

V

K

K

D

D

K

K

N

N

D

S

P

G

F

V

R
x
K

L
|
L

M

M

G
|
G

F
|
F

G
|
G

H
|
H

R
|
R

V

V

Y

Y

K

K

N

N

F

F

D

D

P

P

R
|
R

A

A

K

K

V

I

M

M

K

Q

K

Q

A

T

A

C

D

H

E

E

V

V

L

L

A

T

E

E

L

L

G

G

L

I

E

K

N

D

D

D

P

P

L

L

L

L

A

D

I

L

A

A

K

V

R

E

L

L

E

E

E

K

I

I

A

A

L

L

E

S

D

D

E

D

Y
|
Y

F

F

I

V

E

Q

K
x
R

K

K

L
|
L

Y

Y

P

P

N
|
N

V

V

D
|
D

F

F

Y

Y

S

S

G

G

I

I

I

I

L

L

K

K

A

A

I

M

G

G

I

I

P

P

T

T

S

S

M

M

F

F

T

T

V

V

I

L

F

F

A

A

V

V

G

A

R
|
R

T

T

I

T

G

G

W

W

I

V

A

S

H

Q

W

W

H

K

E

E

M

M

I

I

-

E

S

E

G

P

S

G

Y

Q

R

R

I

I

G

S

R

R

P

P

R

R

Q

Q

L

L

Y

Y

T

I

G

G

H

A

D

P

K

Q

R

R

D

D

F

Y

V

V

P

P

L

L

E

A

D

K

R

R

active site: S232 (= S236), H262 (= H266), H303 (= H307), R312 (= R316), D361 (= D365)
binding carboxymethyldethia coenzyme *a: A261 (≠ S265), H262 (= H266), A265 (= A269), K297 (≠ R301), L298 (= L302), G300 (= G304), F301 (= F305), G302 (= G306), H303 (= H307), R304 (= R308), Y350 (= Y354), R354 (≠ K358), L356 (= L360), N359 (= N363), D361 (= D365)
binding oxaloacetate ion: H227 (= H231), N230 (= N234), H303 (= H307), R312 (= R316), R386 (= R390)

P9WPD5 Citrate synthase 1; EC 2.3.3.16 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
54% identity, 100% coverage: 1:429/429 of query aligns to 1:431/431 of P9WPD5

query
sites
P9WPD5

M

M

S

A

D

D

K

T

K

D

A

D

T

T

L

A

T

T

I

L

D

R

G

Y

M

P

D

G

E

G

A

E

V

I

E

D

L

L

P

Q

I

I

Y

V

S

H

G

A

T

T

I

E

G

G

P

A

D

D

V

G

V

I

D

A

V

L

G

G

S

P

L

L

T

L

G

A

K

K

-

T

G

G

M

H

F

T

T

T

Y

F

D

D

P

V

G

G

F

F

V

A

S

N

T

T

A

A

S

A

C

A

E

K

S

S

Q

S

I

I

T

T

Y

Y

I

I

D

D

G

G

G

D

K

A

G

G

V

I

L

L

L

R

H

Y

R

R

G

G

Y

Y

P

P

I

I

E

D

Q

Q

L

L

A

A

E

E

H

K

S

S

D

T

Y

F

L

I

E

E

T

V

C

C

Y

Y

L

L

L

L

L

I

Y

Y

G

G

E

E

L

L

P

P

T

D

K

T

E

D

Q

Q

K

L

D

A

K

Q

F

F

V

T

S

G

T

R

V

I

T

Q

N

R

H

H

T

T

M

M

V

L

H

H

E

E

Q

D

L

L

A

K

H

R

F

F

F

F

N

D

G

G

F

F

R

P

R

R

D

N

S

A

H

H

P

P

M

M

A

P

I

V

M

L

C

S

G

S

V

V

V

V

G

N

A

A

L

L

S

S

A

A

F

Y

Y

Y

H

Q

D

D

S

A

L

L

D

D

I

P

S

M

D

D

E

N

Q

G

H

Q

R

V

Q

E

V

L

A

S

A

T

F

I

R

R

L

L

I

L

A

A

K

K

M

L

P

P

T

T

L

I

A

A

A

A

M

Y

S

A

Y

Y

K

K

F

K

S

S

I

V

G

G

Q

Q

P

P

F

F

M

L

Y

Y

P

P

D

D

N

N

S

S

M

L

G

T

Y

L

A

V

E

E

N

N

F

F

L

L

H

R

M

L

M

T

F

F

G

G

V

F

P

P

C

A

E

E

K

P

S

Y

N

Q

I

A

S

D

P

P

V

E

L

V

A

V

D

R

A

A

M

L

D

D

K

M

I

L

F

F

L

I

L

L

H

H

A

A

D

D

H

H

E

E

Q

Q

N

N

A

C

S

S

T

T

S

S

T

T

V

V

R

R

L

L

A

V

G

G

S

S

S

S

G

R

A

A

N

N

P

L

F

F

A

T

C

S

I

I

A

S

A

G

G

G

I

I

A

N

A

A

L

L

W

W

G

G

P

P

S

L

H

H

G

G

G

G

A

A

N

N

E

Q

A

A

V

V

L

L

D

E

M

M

L

L

Q

E

E

G

I

I

G

R

D

D

V

S

S

G

-

D

R

D

I

V

D

S

E

E

F

F

V

V

A

R

R

K

A

V

K

K

D

N

K

R

N

E

D

A

P

G

F

V

R

K

L

L

M

M

G

G

F

F

G

G

H

H

R

R

V

V

Y

Y

K

K

N

N

F

Y

D

D

P

P

R

R

A

A

K

R

V

I

M

V

K

K

K

E

A

Q

A

A

D

D

E
x
K

V

I

L

L

A

A

E

K

L

L

G

G

L

-

E

G

N

D

D

D

P

S

L

L

L

L

A

G

I

I

A

A

K

K

R

E

L

L

E

E

E

E

I

A

A

A

L

L

E

T

D

D

E

D

Y

Y

F

F

I

I

E

E

K

R

K

K

L

L

Y

Y

P

P

N

N

V

V

D

D

F

F

Y

Y

S

T

G

G

I

L

I

I

L

Y

K

R

A

A

I

L

G

G

I

F

P

P

T

T

S

R

M

M

F

F

T

T

V

V

I

L

F

F

A

A

V

L

G

G

R

R

T

L

I

P

G

G

W

W

I

I

A

A

H

H

W

W

H

R

E

E

M

M

-

H

I

D

S

E

G

G

S

D

Y

S

R

K

I

I

G

G

R

R

P

P

R

R

Q

Q

L

I

Y

Y

T

T

G

G

H

Y

D

T

K

E

R

R

D

D

F

Y

V

V

P

T

L

I

E

D

D

A

R

R

K328 (≠ E326) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

3msuB Crystal structure of citrate synthase from francisella tularensis
56% identity, 98% coverage: 4:423/429 of query aligns to 10:426/426 of 3msuB

query
sites
3msuB

K

K

K

Y

A

A

T

T

L

L

T

K

I

Y

D

A

G

D

M

K

D

N

E

I

A

E

V

I

E

E

L

L

P

P

I

V

Y

Y

S

S

G

P

T

S

I

L

G

G

P

N

D

D

V

C

V

I

D

D

V

V

G

S

S

S

L

L

T

V

G

K

K

H

G

G

M

I

F

F

T

T

Y

Y

D

D

P

P

G

G

F

F

V

M

S

S

T

T

A

A

S

A

C

C

E

E

S

S

Q

K

I

I

T

T

Y

Y

I

I

D

D

G

G

G

G

K

K

G

G

V

V

L

L

L

L

H

H

R

R

G

G

Y

Y

P

P

I

I

E

E

Q

E

L

W

A

T

E

Q

H

K

S

S

D

N

Y
|
Y

L
x
R

E

T

T

L

C
|
C

Y

Y

L

A

L

L

L

I

Y

Y

G

G

E

E

L

L

P

P

T

T

K

D

E

E

Q

Q

K

V

D

K

K

S

F

F

V
x
R

S

Q

T

E

V

I

T

I

N

N

H

K

T

M

M

P

V

V

H

C

E

E

Q

H

L

V

A

K

H

A

F

A

F

I

N

A

G

A

F

M

R

P

R

Q

D

H

S

T

H

H

P

P

M

M

A

S

I

S

M

L

C

I

G

A

V

G

V

V

G

N

A

V

L

L

S

A

A

A

F

-

Y

-

H

-

D

E

S

H

L

I

D

H

I

N

S

G

D

Q

E

K

Q

E

H

S

R

Q

Q

D

V

E

A

V

A

A

F

K

R

N

L

I

I

V

A

A

K

K

M

I

P

A

T

T

L

I

A

A

A

A

M

M

S

A

Y

Y

K

R

F

H

S

N

I

H

G

G

Q

K

P

K

F

F

M

L

Y

E

P

P

D

K

N

M

S

E

M

Y

G

G

Y
|
Y

A

A

E

E

N

N

F

F

L

L

H

Y

M

M

M

M

F

F

G

A

V

-

P

D

C

D

E

E

K

S

S

Y

N

K

I

P

S

D

P

E

V

L

L

H

A

I

D

K

A

A

M

M

D

D

K

T

I

I

F

F

L

M

L

L

H

H

A

A

D

D

H

H

E

E

Q

Q

N

N

A

A

S
|
S

T

T

S

S

T

T

V

V

R

R

L

L

A

S

G

G

S

S

S

T

G

G

A

N

N

S

P

P

F

Y

A

A

C

A

I

I

A

I

A

A

G

G

I

I

A

T

A

A

L

L

W

W

G

G

P

P

S

A

H
|
H

G

G

G

G

A

A

N

N

E

E

A

A

V

V

L

L

D

K

M

M

L

L

Q

S

E

E

I

I

G

G

D

S

V

T

S

E

R

N

I

I

D

D

E

K

F

Y

V

I

A

A

R

K

A

A

K

K

D

D

K

K

N

D

D

D

P

P

F

F

R

R

L

L

M

M

G

G

F

F

G

G

H
|
H

R

R

V

V

Y

Y

K

K

N

N

F

T

D

D

P

P

R
|
R

A

A

K

T

V

A

M

M

K

K

K

K

A

N

A

C

D

E

E

E

V

I

L

L

A

A

E

K

L

L

G

G

L

H

E

S

N

D

D

N

P

P

L

L

L

L

A

T

I

V

A

A

K

K

R

K

L

L

E

E

E

E

I

I

A

A

L

L

E

Q

D

D

E

E

Y

F

F

F

I

I

E

E

K

R

K

K

L

L

Y

F

P

S

N

N

V

V

D
|
D

F

F

Y

Y

S

S

G

G

I

I

I

I

L

L

K

K

A

A

I

M

G

G

I

I

P

P

T

E

S

D

M

M

F

F

T

T

V

A

I

I

F

F

A

A

V

L

G

A

R

R

T

T

I

S

G

G

W

W

I

I

A

S

H

Q

W

W

H

I

E

E

M

M

I

V

S

N

G

D

-

P

S

A

Y

Q

R

K

I

I

G

G

R

R

P

P

R

R

Q

Q

L

L

Y

Y

T

T

G

G

H

A

D

T

K

N

R

R

D

N

F

F

active site: S238 (= S236), H268 (= H266), H309 (= H307), R318 (= R316), D367 (= D365)
binding oxaloacetate ion: Y90 (= Y84), R91 (≠ L85), C94 (= C88), R112 (≠ V106), Y198 (= Y195)

3msuA Crystal structure of citrate synthase from francisella tularensis
54% identity, 98% coverage: 4:423/429 of query aligns to 10:415/415 of 3msuA

query
sites
3msuA

K

K

K

Y

A

A

T

T

L

L

T

K

I

Y

D

A

G

D

M

K

D

N

E

I

A

E

V

I

E

E

L

L

P

P

I

V

Y

Y

S

S

G

P

T

S

I

L

G

G

P

N

D

D

V

C

V

I

D

D

V

V

G

S

S

S

L

L

T

V

G

K

K

H

G

G

M

I

F

F

T

T

Y

Y

D

D

P

P

G

G

F

F

V

M

S

S

T

T

A

A

S

A

C

C

E

E

S

S

Q

K

I

I

T

T

Y

Y

I

I

D

D

G

G

G

G

K

K

G

G

V

V

L

L

L

L

H

H

R

R

G

G

Y

Y

P

P

I

I

E

E

Q

E

L

W

A

T

E

Q

H

K

S

S

D

N

Y
|
Y

L
x
R

E

T

T

L

C

C

Y

Y

L

A

L

L

L

I

Y

Y

G

G

E

E

L

L

P

P

T

T

K

D

E

E

Q

Q

K

V

D

K

K

S

F

F

V
x
R

S

Q

T

E

V

I

T

I

N

N

H

K

T

M

M

P

V

V

H
x
C

E

E

Q
x
H

L

V

A

K

H

A

F

A

F

I

N

A

G

A

F

M

R

P

R

Q

D

H

S

T

H

H

P

P

M

M

A

S

I

S

M

L

C

I

G

A

V

G

V

V

G

N

A

V

L

L

S

A

A

A

F

-

Y

-

H

-

D

E

S

H

L

I

D

H

I

N

S

G

D

Q

E

K

Q

E

H

S

R

Q

Q

D

V

E

A

V

A

A

F

K

R

N

L

I

I

V

A

A

K

K

M

I

P

A

T

T

L

I

A

A

A

A

M

M

S

A

Y

Y

K

R

F

H

S

N

I

H

G

G

Q

K

P

K

F

F

M

L

Y

E

P

P

D

K

N

M

S

E

M

Y

G

G

Y
|
Y

A

A

E

E

N

N

F

F

L

L

H

Y

M

M

M

M

F

F

G

A

V

-

P

D

C

D

E

E

K

S

S

Y

N

K

I

P

S

D

P

E

V

L

L

H

A

I

D

K

A

A

M

M

D
|
D

K

T

I

I

F

F

L

M

L

L

H

H

A

A

D

D

H

H

E

E

Q

Q

N

N

A

A

S
|
S

T

T

S

S

T

T

V

V

R

R

L

L

A

S

G

G

S

S

S

T

G

G

A

N

N

S

P

P

F

Y

A

A

C

A

I

I

A

I

A

A

G

G

I

I

A

T

A

A

L

L

W

W

G

G

P

P

S

A

H
|
H

G

G

G

G

A

A

N

N

E

E

A

A

V

V

L

L

D

K

M

M

L

L

Q

S

E

E

I

I

G

G

D

S

V

T

S

E

R

N

I

I

D

D

E

K

F

Y

V

I

A

A

R

K

A

A

K

K

D

D

K

K

N

-

D

-

P

-

F

-

R

-

L

L

M

M

G

G

F

F

G

G

H
|
H

R

R

V

V

Y

Y

K

K

N

N

F

T

D

D

P

P

R
|
R

A

A

K

T

V

A

M

M

K

K

K

K

A

N

A

C

D

E

E

E

V

I

L

L

A

A

E

K

L

L

G

G

L

H

E

S

N

D

D

N

P

P

L

L

L

L

A

T

I

V

A

A

K

K

R

K

L

L

E

E

E

E

I

I

A

A

L

L

E

-

D

-

E

-

Y

-

F

-

I

-

E

E

K

R

K

K

L

L

Y

F

P

S

N

N

V

V

D
|
D

F

F

Y

Y

S

S

G

G

I

I

I

I

L

L

K

K

A

A

I

M

G

G

I

I

P

P

T

E

S

D

M

M

F

F

T

T

V

A

I

I

F

F

A

A

V

L

G

A

R

R

T

T

I

S

G

G

W

W

I

I

A

S

H

Q

W

W

H

I

E

E

M

M

I

V

S

N

G

D

-

P

S

A

Y

Q

R

K

I

I

G

G

R

R

P

P

R

R

Q

Q

L

L

Y

Y

T

T

G

G

H

A

D

T

K

N

R

R

D

N

F

F

active site: S238 (= S236), H268 (= H266), H304 (= H307), R313 (= R316), D356 (= D365)
binding oxaloacetate ion: Y90 (= Y84), R91 (≠ L85), R112 (≠ V106), Y198 (= Y195), D224 (= D222)
binding zinc ion: C122 (≠ H116), H124 (≠ Q118)

4tvmA Structure of citrate synthase from mycobacterium tuberculosis (see paper)
49% identity, 96% coverage: 17:426/429 of query aligns to 11:380/380 of 4tvmA

query
sites
4tvmA

V

I

E

D

L

L

P

Q

I

I

Y

V

S

H

G

A

T

T

I

E

G

G

P

A

D

D

V

G

V

I

D

A

V

L

G

G

S

P

L

L

T

L

G

A

K

K

-

T

G

G

M

H

F

T

T

T

Y

F

D

D

P

V

G

G

F

F

V

A

S

N

T

T

A

A

S

A

C

A

E

K

S

S

Q

S

I

I

T

T

Y

Y

I

I

D

D

G

G

G

D

K

A

G

G

V

I

L

L

L

R

H

Y

R

R

G

G

Y

Y

P

P

I

I

E

D

Q

Q

L

L

A

A

E

E

H

K

S

S

D

T

Y

F

L

I

E

E

T

V

C

C

Y

Y

L

L

L

L

L

I

Y

Y

G

G

E

E

L

L

P

P

T

D

K

T

E

D

Q

Q

K

L

D

A

K

Q

F

F

V

T

S

G

T

R

V

I

T

Q

N

R

H

H

T

T

M

M

V

L

H

H

E

E

Q

D

L

L

A

K

H

R

F

F

F

F

N

D

G

G

F

F

R

P

R

R

D

N

S

A

H

H

P

P

M

M

A

P

I

V

M

L

C

S

G

S

V

V

V

V

G

N

A

A

L

L

S

S

A

A

F

Y

Y

Y

H

Q

D

D

S

A

L

L

D

D

I

P

S

M

D

D

E

N

Q

G

H

Q

R

V

Q

E

V

L

A

S

A

T

F

I

R

R

L

L

I

L

A

A

K

K

M

L

P

P

T

T

L

I

A

A

A

A

M

Y

S

A

Y

Y

K

K

F

K

S

S

I

V

G

G

Q

Q

P

P

F

F

M

L

Y

Y

P

P

D

D

N

N

S

S

M

L

G

T

Y

L

A

V

E

E

N

N

F

F

L

L

H

R

M

L

M

T

F

F

G

G

V

F

P

P

C

A

E

E

K

P

S

Y

N

Q

I

A

S

D

P

P

V

E

L

V

A

V

D

R

A

A

M

L

D

D

K

M

I

L

F

F

L

I

L

L

H

H

A

A

D

D

H
|
H

E

E

Q

Q

N
|
N

A

C

S
|
S

T

T

S

S

T

T

V

V

R

R

L

L

A

V

G

G

S

S

S

S

G

R

A

A

N

N

P

L

F

F

A

T

C

S

I

I

A

S

A

G

G

G

I

I

A

N

A

A

L

L

W

W

G

G

P

P

S

L

H
|
H

G

G

G

G

A

A

N

N

E

Q

A

A

V

V

L

L

D

E

M

M

L

L

Q

E

E

G

I

I

G

R

D

D

V

S

S

-

R

-

I

-

D

-

E

-

F

-

V

-

A

-

R

-

A

-

K

-

D

-

K

-

N

-

D

-

P

-

F

-

R

-

L

-

M

-

G

-

F

-

G

G

H

D

R

D

V

V

Y

S

K

E

N

N

F

Y

D

D

P

P

R
|
R

A

A

K

R

V

I

M

V

K

K

K

E

A

Q

A

A

D

D

E

K

V

I

L

L

A

G

E

D

L

-

G

-

L

-

E

-

N

-

D

D

P

S

L

L

L

L

A

G

I

I

A

A

K

K

R

E

L

L

E

E

E

E

I

A

A

-

L

-

E

-

D

-

E

-

Y

-

F

-

I

-

E

-

K

-

K

-

L

-

Y

-

P

-

N

-

V

V

D
|
D

F

F

Y

Y

S

T

G

G

I

L

I

I

L

Y

K

R

A

A

I

L

G

G

I

F

P

P

T

T

S

R

M

M

F

F

T

T

V

V

I

L

F
|
F

A

A

V

L

G

G

R
|
R

T

L

I

P

G

G

W

W

I

I

A

A

H

H

W

W

H

R

E

E

M

M

-

H

I

D

S

E

G

G

S

D

Y

S

R

K

I

I

G

G

R

R

P

P

R

R

Q

Q

L

I

Y

Y

T

T

G

G

H

Y

D

T

K

E

R

R

D

D

F

Y

V

V

P

T

L

I

active site: S231 (= S236), H261 (= H266), R289 (= R316), D318 (= D365)
binding oxaloacetate ion: H226 (= H231), N229 (= N234), F339 (= F386), R343 (= R390)

8bp7E Citrate-bound hexamer of synechococcus elongatus citrate synthase (see paper)
38% identity, 90% coverage: 44:429/429 of query aligns to 5:377/379 of 8bp7E

query
sites
8bp7E

Y

F

D

R

P

P

G

G

F

L

V

E

S

G

T

V

A

P

S

A

C

T

E

L

S

S

Q

S

I

I

T

S

Y

F

I

V

D

D

G

G

G

Q

K

R

G

G

V

V

L

L

L

E

H

Y

R

R

G

G

Y

I

P

S

I

I

E

E

Q

Q

L

L

A

A

E

Q

H

Q

S

S

D

S

Y

F

L

L

E

E

T

T

C

A

Y

Y

L

L

L

L

L

I

Y

W

G

G

E

H

L
|
L

P
|
P

T

T

K

Q

E

Q

Q

E

K

L

D

T

K

E

F

F

V

E

S

H

T

E

V

I

T

R

N

Y

H

H

T

R

M

R

V

I

H

K

E

F

Q

R

L

I

A

R

H

D

F

M

F

M

N

K

G

C

F

F

R

P

R

D

D

S

S

G

H

H

P

P

M

M

A

D

I

A

M

L

C

Q

G

A

V

S

V

A

G

A

A

A

L

L

S

G

A

L

F

F

Y

Y

-

S

H

R

D

R

S

A

L

L

D

D

I

-

S

-

D

D

E

P

Q

E

H

Y

R

I

Q

R

V

A

A

A

A

V

F

V

R

R

L

L

I

L

A

A

K

K

M

I

P

P

T

T

L

M

A

V

A

A

M

A

S

F

Y

Q

K

L

F

I

S

R

I

K

G

G

Q

N

P

D

F

P

M

I

Y

Q

P

P

D

R

N

D

S

E

M

L

G

D

Y

Y

A

A

E

A

N

N

F

F

L

L

H

Y

M

M

M

L

F

-

G

-

V

-

P

-

C

-

E

T

K

E

S

R

N

E

I

P

S

D

P

P

V

V

L

A

A

A

D

R

A

I

M

F

D

D

K

I

I

C

F

L

L

T

L

L

H

H

A

A

D

E

H

H

E

T

Q

I

N

N

A

A

S

S

T

T

S

F

T

S

V

A

R

M

L

V

A

T

G

A

S

S

S

T

G

L

A

T

N

D

P

P

F

Y

A

A

C

V

I

V

A

A

A

S

G

A

I

V

A

G

A

T

L

L

W

A

G

G

P

P

S

L

H

H

G

G

G

G

A

A

N

N

E

E

A

E

V

V

L

L

D

D

M

M

L

L

Q

E

E

A

I

I

G

G

D

S

V

V

S

E

R

N

I

V

D

E

E

P

F

Y

V

L

A

D

R

H

A

C

K

I

D

A

K

T

N

K

D

T

P

-

F

-

R

R

L

I

M

M

G

G

F

F

G

G

H

H

R

R

V

V

Y

Y

K

K

N

V

F

K

D

D

P

P

R

R

A

A

K

V

V

I

M

L

K

Q

K

N

A

L

A

A

D

E

E

Q

V

L

L

F

A

D

E

I

L

F

G

G

L

-

E

-

N

H

D

D

P

P

L

Y

L

Y

A

E

I

I

A

A

K

V

R

A

L

V

E

E

E

K

I

A

A

A

L

A

E

E

D

R

E

-

Y

-

F

L

I

S

E

H

K

K

K

G

L

I

Y

Y

P

P

N

N

V

V

D

D

F

F

Y

Y

S

S

G

G

I

L

I

V

L

Y

K

R

A

K

I

L

G

G

I

I

P

P

T

S

S

D

M

L

F

F

T

T

V

P

I

V

F

F

A

A

V

I

G

A

R

R

T

V

I

A

G

G

W

W

I

L

A

A

H

H

W

W

H

K

E

E

M

Q

I

L

S

N

G

E

S

N

Y

-

R

R

I

I

G

F

R

R

P

P

R

T

Q

Q

L

I

Y

Y

T

T

G

G

H

S

D

H

K

N

R

L

D

D

F

Y

V

T

P

P

L

I

E