SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_084199764.1 NCBI__GCF_002869505.1:WP_084199764.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P22033 Methylmalonyl-CoA mutase, mitochondrial; MCM; Methylmalonyl-CoA isomerase; EC 5.4.99.2 from Homo sapiens (Human) (see 28 papers)
67% identity, 97% coverage: 17:714/719 of query aligns to 43:739/750 of P22033

query
sites
P22033

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I

Q50 (= Q24) binding malonyl-CoA
I69 (≠ V43) to V: in MMAM; likely benign; dbSNP:rs115923556
P86 (= P62) to L: in MMAM; mut0 and mut-; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs769348060
G87 (= G63) to E: in MMAM; mut0; dbSNP:rs1554160986
R93 (= R69) to H: in MMAM; mut0; decreased methylmalonyl-CoA mutase activity; dbSNP:rs121918251
G94 (= G70) to R: in MMAM; mut0; dbSNP:rs727504022; to V: in MMAM; mut- and mut0; dbSNP:rs535411418
P95 (= P71) to R: in MMAM; mut0; dbSNP:rs190834116
YPTM 96:99 (≠ QATM 72:75) binding malonyl-CoA
Y100 (= Y76) to C: in MMAM; mut-; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs864309735
W105 (= W81) to R: in MMAM; mut0; dbSNP:rs121918249
TIRQY 106:110 (= TIRQY 82:86) binding malonyl-CoA
R108 (= R84) to C: in MMAM; mut0; dbSNP:rs121918257; to G: in MMAM; mut-; dbSNP:rs121918257; to H: in MMAM; mut0; dbSNP:rs483352778
Q109 (= Q85) to R: in MMAM; mut0; dbSNP:rs1461110052
G133 (= G109) to R: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs879253828
A137 (= A113) to V: in MMAM; mut0; dbSNP:rs941483851
D139 (= D115) to N: in MMAM; uncertain significance; dbSNP:rs879253829
L140 (= L116) to P: in MMAM; decreased protein expression; decreased methylmalonyl-CoA mutase activity
A141 (= A117) to T: in MMAM; decreased protein expression; dbSNP:rs1554160730
H143 (= H119) to Y: in MMAM; mut0
G145 (= G121) to S: in MMAM; mut0; dbSNP:rs1237080100
S148 (= S124) to L: in MMAM; mut0; dbSNP:rs1300547552
D156 (= D132) to N: in MMAM; mut-
G158 (= G134) to V: in MMAM; mut0
G161 (= G137) to R: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs2127420039; to V: in MMAM; decreased protein expression
F174 (= F150) to S: in MMAM; mut0; dbSNP:rs864309733
M186 (= M162) to V: in MMAM; mut-; dbSNP:rs148331800
T187 (= T163) to S: in MMAM; mut0; dbSNP:rs879253830
N189 (= N165) to I: in MMAM; mut-; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs200908035; to K: in MMAM; mut-; dbSNP:rs1561959114
A191 (= A167) to E: in MMAM; mut- and mut0; affects proper folding; reduced protein level; decreased methylmalonyl-CoA mutase activity; dbSNP:rs760782399
A197 (= A173) to E: in MMAM; mut0
G203 (≠ A179) to R: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs778702777
E205 (= E181) natural variant: Missing (in MMAM; mut0; dbSNP:rs879253831)
G215 (= G191) to C: in MMAM; mut- and mut0; dbSNP:rs121918258; to S: in MMAM; mut0; dbSNP:rs121918258
TIQ 216:218 (= TIQ 192:194) binding malonyl-CoA
Q218 (= Q194) to H: in MMAM; mut0 and mut-; no effect on protein abundance; loss of methylmalonyl-CoA mutase activity; alters thermodynamic stability; dbSNP:rs1446389693
N219 (= N195) to Y: in MMAM; mut0; no effect on protein abundance; loss of methylmalonyl-CoA mutase activity; dbSNP:rs121918256
R228 (= R204) binding malonyl-CoA; to Q: in MMAM; mut0; dbSNP:rs770810987
T230 (= T206) to I: in MMAM; mut-; dbSNP:rs879253833; to R: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs879253833
Y231 (= Y207) to N: in MMAM; mut-; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity; strong decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs864309736
K255 (= K231) binding malonyl-CoA
S262 (= S238) to N: in MMAM; mut0; dbSNP:rs1767683356
H265 (= H241) binding malonyl-CoA; to Y: in MMAM; mut-
E276 (= E252) to D: in MMAM; uncertain significance; mut-; dbSNP:rs12175488
L281 (= L257) to S: in MMAM; mut0; dbSNP:rs796052007
G284 (= G260) to E: in MMAM; mut0; dbSNP:rs879253835; to R: in MMAM; mut0; dbSNP:rs761477436
S288 (≠ I264) to P: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs1179778233
G291 (≠ A267) to E: in MMAM; mut0
Q293 (≠ A269) to P: in MMAM; mut0; dbSNP:rs138374956
RLS 304:306 (= RLS 280:282) binding malonyl-CoA
L305 (= L281) to S: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs1554160246
S306 (= S282) to F: in MMAM; mut0; dbSNP:rs1085307929
W309 (= W285) to G: in MMAM; decreased protein expression; dbSNP:rs2127418704
G312 (= G288) to V: in MMAM; mut0; dbSNP:rs864309734
Y316 (= Y292) to C: in MMAM; mut-; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity; no decreased affinity for adenosylcob(III)alamin; dbSNP:rs781474200
A324 (= A300) to T: in MMAM; mut-; dbSNP:rs780387525
R326 (= R302) to K: in MMAM; uncertain significance; dbSNP:rs758577372
L328 (= L304) to F: in MMAM; mut0; affects proper folding; no effect on protein abundance; loss of methylmalonyl-CoA mutase activity; dbSNP:rs796052002; to P: in MMAM; mut0; dbSNP:rs965316043
S344 (= S319) to F: in MMAM; mut-; affects proper folding; no effect on protein abundance; loss of methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin
L346 (≠ M321) natural variant: Missing (in MMAM; mut0)
L347 (= L322) to R: in MMAM; mut0; dbSNP:rs1026703654
H350 (= H325) to Y: in MMAM; mut0; dbSNP:rs1407914109
L358 (= L333) to P: in MMAM; mut0; dbSNP:rs1767612147
N366 (= N341) to S: in MMAM; mut-; no effect on protein abundance; loss of methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs864309737
R369 (= R344) to C: in MMAM; mut0; dbSNP:rs772552898; to H: in MMAM; mut- and mut0; no effect on protein abundance; loss of methylmalonyl-CoA mutase activity; alters thermodynamic stability; dbSNP:rs564069299
T370 (= T345) to P: in MMAM; mut0; dbSNP:rs368790885
A377 (= A352) to E: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs121918250
Q383 (= Q358) to H: in MMAM; mut0; to P: in MMAM; mut0
H386 (= H361) to N: in MMAM; mut0; dbSNP:rs1554159937; to R: in MMAM; mut0; dbSNP:rs866933356
T387 (= T362) to I: in MMAM; mut-; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs1400022403
N388 (= N363) to H: in MMAM; mut0; dbSNP:rs766010704; to K: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs879253840
S389 (≠ A364) natural variant: Missing (in MMAM; mut0)
I412 (= I387) natural variant: Missing (in MMAM; mut0)
P424 (= P399) to L: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs879253842
G426 (= G401) to E: in MMAM; mut-; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs533755473; to R: in MMAM; mut-; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity; strong decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs769922244
G427 (= G402) to D: in MMAM; mut0; dbSNP:rs753288303
G454 (= G429) to E: in MMAM; mut0
A499 (= A474) to T: in dbSNP:rs2229385
I505 (≠ V480) to T: in MMAM; decreased protein expression; decreased methylmalonyl-CoA mutase activity
Q514 (= Q489) to E: in MMAM; uncertain significance; to K: in MMAM; decreased protein expression; dbSNP:rs2127416800
L518 (= L493) to P: in MMAM; mut0; dbSNP:rs864309738
R532 (≠ A507) to H: in dbSNP:rs1141321
A535 (≠ E510) to P: in MMAM; mut0; dbSNP:rs760183775
A552 (= A527) to V: in MMAM; uncertain significance; dbSNP:rs879253845
C560 (≠ A535) to Y: in MMAM; mut0; dbSNP:rs1238333040
T566 (≠ S541) to R: in MMAM; mut0; dbSNP:rs1767288356
F573 (= F548) to S: in MMAM; mut-; affects proper folding; no effect on protein abundance; no effect on methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; does not alter thermodynamic stability; dbSNP:rs775593146
Y587 (= Y562) to C: in MMAM; mut-
I597 (≠ W572) to R: in MMAM; no changed in protein expression; decreased methylmalonyl-CoA mutase activity; dbSNP:rs1554158951
P615 (= P590) to L: in MMAM; mut0; affects proper folding; reduced strongly protein level; dbSNP:rs1554158777; to R: in MMAM; mut0; dbSNP:rs1554158777; to T: in MMAM; mut0; affects proper folding; reduced strongly protein level; loss of methylmalonyl-CoA mutase activity; dbSNP:rs1302409621
R616 (= R591) to C: in MMAM; mut0; dbSNP:rs765284825
L617 (≠ M592) to R: in MMAM; mut0; dbSNP:rs1554158775
K621 (= K596) to N: in MMAM; mut0
G623 (= G598) to R: in MMAM; mut0; dbSNP:rs121918254
Q624 (= Q599) to R: in MMAM; no effect on protein abundance; dbSNP:rs768521956
D625 (= D600) to G: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs879253847; to V: in MMAM; mut0; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity
G626 (= G601) to C: in MMAM; mut-; dbSNP:rs982110849
H627 (= H602) binding axial binding residue; to R: in MMAM; mut0; dbSNP:rs372486357
G630 (= G605) to E: in MMAM; mut0; dbSNP:rs143023066
V633 (= V608) to G: in MMAM; mut-; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; does not alter thermodynamic stability; dbSNP:rs200055428
G637 (≠ A612) to E: in MMAM; mut-; to R: in MMAM; mut0; dbSNP:rs781501004
F638 (= F613) to I: in MMAM; mut0
D640 (= D615) to Y: in MMAM; mut0; dbSNP:rs865815395
G642 (= G617) to R: in MMAM; mut-; dbSNP:rs747897332
G648 (≠ S623) to D: in MMAM; mut-; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity; strong decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs766721811
V669 (= V644) to E: in MMAM; mut0; dbSNP:rs1360470463
I671 (≠ A646) to V: in dbSNP:rs8589
L674 (= L649) to F: in MMAM; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs1164271240
H678 (= H653) to R: in MMAM; mut-; dbSNP:rs147094927
E684 (= E659) natural variant: E -> EL (in MMAM; mut-)
L685 (= L660) to R: in MMAM; mut-; dbSNP:rs864309739
R694 (≠ A669) to L: in MMAM; mut-; decreased protein abundance; loss of methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs756225782; to W: in MMAM; mut- and mut0; no effect on protein abundance; loss of methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs777758903
M700 (≠ I675) to K: in MMAM; mut-; no effect on protein abundance; loss of methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs140600746
G703 (= G678) to R: in MMAM; mut0; dbSNP:rs121918255
G717 (= G692) to V: in MMAM; mut-; no effect on protein abundance; interferes with the binding of the cofactor to the apoenzyme; decreased methylmalonyl-CoA mutase activity; strong decreased affinity for adenosylcob(III)alamin; decreased thermodynamic stability; dbSNP:rs121918252
G723 (= G698) to D: in MMAM; decreased protein expression; decreased methylmalonyl-CoA mutase activity; dbSNP:rs755077681

Sites not aligning to the query:

1:32 modified: transit peptide, Mitochondrion
7:750 natural variant: Missing (in MMAM; mut-)
152:750 natural variant: Missing (in MMAM; mut0)
228:750 natural variant: Missing (in MMAM; mut0)

2xiqA Crystal structure of human methylmalonyl-coa mutase in complex with adenosylcobalamin and malonyl-coa (see paper)
67% identity, 97% coverage: 17:714/719 of query aligns to 8:704/714 of 2xiqA

query
sites
2xiqA

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active site: Y75 (= Y86), Y229 (= Y240), H230 (= H241), K586 (= K596), D590 (= D600), H592 (= H602)
binding cobalamin: Y75 (= Y86), L105 (= L116), H108 (= H119), A125 (= A136), R193 (= R204), E233 (= E244), G320 (= G330), W321 (= W331), E357 (= E367), G360 (≠ A370), L361 (= L371), G591 (= G601), H592 (= H602), D593 (= D603), R594 (= R604), G595 (= G605), I599 (≠ V609), G635 (= G645), S637 (= S647), L639 (= L649), A641 (= A651), G667 (= G677), G668 (= G678), F687 (= F697), G688 (= G698), T691 (= T701)
binding malonyl-coenzyme a: Y61 (≠ Q72), T63 (= T74), M64 (= M75), R68 (= R79), T71 (= T82), R73 (= R84), Y75 (= Y86), S150 (= S161), T152 (= T163), T181 (= T192), R193 (= R204), K220 (= K231), H230 (= H241), R269 (= R280), S271 (= S282), F273 (= F284), R313 (= R323), A314 (≠ T324), H315 (= H325), Q317 (= Q327), Q348 (= Q358)

8dyjB Crystal structure of human methylmalonyl-coa mutase in complex with adp and cob(ii)alamin (see paper)
67% identity, 97% coverage: 17:714/719 of query aligns to 7:703/708 of 8dyjB

query
sites
8dyjB

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I

binding adenosine-5'-diphosphate: Y74 (= Y86), T151 (= T163), R192 (= R204), Y228 (= Y240), H229 (= H241), F272 (= F284), Q316 (= Q327), N352 (= N363), E356 (= E367), L360 (= L371), P361 (= P372)
binding cobalamin: F102 (= F114), L104 (= L116), H107 (= H119), A124 (= A136), V191 (= V203), R192 (= R204), H229 (= H241), E232 (= E244), G319 (= G330), W320 (= W331), E356 (= E367), G359 (≠ A370), L360 (= L371), G590 (= G601), H591 (= H602), D592 (= D603), R593 (= R604), G594 (= G605), I598 (≠ V609), S636 (= S647), L638 (= L649), A640 (= A651), G666 (= G677), G667 (= G678), V668 (= V679), F686 (= F697), G687 (= G698), T690 (= T701)

8gjuJ Crystal structure of human methylmalonyl-coa mutase (mmut) in complex with methylmalonic acidemia type a protein (mmaa), coenzyme a, and gdp (see paper)
65% identity, 97% coverage: 17:714/719 of query aligns to 8:681/689 of 8gjuJ

query
sites
8gjuJ

W

W

A

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