SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_084200976.1 NCBI__GCF_002869505.1:WP_084200976.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

3u9sF Crystal structure of p. Aeruginosa 3-methylcrotonyl-coa carboxylase (mcc) 750 kd holoenzyme, coa complex (see paper)
72% identity, 100% coverage: 1:535/535 of query aligns to 3:537/537 of 3u9sF

query
sites
3u9sF

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active site: A150 (= A148), A192 (= A190), G193 (= G191), F381 (= F379), A421 (= A419), G422 (= G420)
binding 5-(hexahydro-2-oxo-1h-thieno[3,4-d]imidazol-6-yl)pentanal: T379 (= T377), G380 (= G378), F381 (= F379), A421 (= A419), M447 (= M445)
binding coenzyme a: R49 (= R47), K115 (= K113), T118 (= T116), A150 (= A148), N151 (≠ F149), L152 (= L150)

8k2vG 3-methylcrotonyl-coa carboxylase in mccd state with acetyl coa
67% identity, 98% coverage: 13:535/535 of query aligns to 18:541/541 of 8k2vG

query
sites
8k2vG

A

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binding acetyl coenzyme *a: K119 (= K113), G120 (= G114), G152 (= G146), G153 (= G147), A154 (= A148), L156 (= L150), T195 (= T189)
binding 5-(hexahydro-2-oxo-1h-thieno[3,4-d]imidazol-6-yl)pentanal: F385 (= F379), V387 (= V381)

8j4zJ Human 3-methylcrotonyl-coa carboxylase in bccp-cts state with substrate
67% identity, 98% coverage: 13:535/535 of query aligns to 18:541/541 of 8j4zJ

query
sites
8j4zJ

A

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A

D

D

E

E

S

N

I

I

I

I

V

V

K

R

E

K

Q

Q

G

G

T

T

I

I

F

F

L
|
L

G

A

G

G

P

P

P

P

L
|
L

V

V

K

K

A

A

A

A

T

T

G

G

E

E

I

E

V

V

S

S

A

A

E

E

E

D

L

L

G

G

G

G

A

A

D

D

V

L

H

H

C

C

R

R

T

K

S

S

G

G

V

V

T

S

D

D

H

H

Y

W

A

A

N

L

N

D

D

D

H

H

H

H

A

A

L

L

E

H

L

L

V

T

R

R

R

K

S

V

V

V

G

R

R

N

L

L

N

N

R

Y

V

Q

K

K

Q

K

I

L

D

D

I

V

D

T

V

I

R

E

D

P

S

S

I

E

E

E

P

P

A

L

F

F

P

P

V

A

E

D

D

E

I

L

Y

Y

G

G

V

I

I

V

P

G

A

A

D

N

A

L

K

K

Q

R

P

S

Y

F

D

D

V

V

R

R

E

E

V

V

I

I

A

A

R

R

V

I

V

V

D

D

G

G

S

S

E

R

F

F

D

T

E

E

F

F

K

K

A

A

L

F

Y

Y

G

G

E

D

T

T

L

L

V

V

C

T

G

G

F

F

A

A

R

R

I

I

H

F

G

G

Y

Y

P

P

V

V

G

G

I

I

I

V

A

G

N

N

N

N

G

G

I

V

L

L

F

F

A

S

E

E

S

S

S

A

V

K

K

K

G

G

A

T

H

H

F

F

V

V

E

Q

L

L

C

C

A

C

Q

Q

R

R

K

N

I

I

P

P

L

L

V

L

F

F

L

L

Q

Q

N

N

I

I

T

T

G
|
G

F
|
F

M

M

V

V

G

G

K

R

Q

E

Y

Y

E

E

H

A

G

E

G

G

I

I

A

A

R

K

H

D

G

G

A

A

K

K

M

M

V

V

H

A

A

A

V

V

A

A

C

C

A

A

E

Q

V

V

P

P

K

K

F

I

T

T

V

L

I

I

I

I

G

G

G

G

S

S

F
x
Y

G

G

A
|
A

G

G

N

N

Y

Y

A

G

M

M

C

C

G

G

R

R

A

A

Y

Y

E

S

P

P

R

R

F

F

M

L

F

Y

M

I

W

W

P

P

N

N

A

A

R

R

I

I

S

S

V

V

M

M

G

G

G

G

E

E

Q

Q

A

A

A

A

G

N

V

V

L

L

A

A

T

T

V
x
I

K

T

Q

K

E

D

Q
|
Q

L

R

A

A

R

R

E

E

G

G

K

K

A

Q

M

F

T

S

E

S

A

A

E

D

E

E

A

A

A

A

F

L

K

K

Q

E

P

P

T

I

L

I

E

K

L

K

Y

F

E

E

K

E

Q

E

G

G

H

N

P

P

Y

Y

Y

Y

A

S

S

S

A

A

R

R

L

V

W

W

D

D

D

D

G

G

V

I

I

I

D

D

P

P

A

A

D

D

T

T

R

R

M

L

V

V

L

L

G

G

L

L

S

S

L

F

S

S

A

A

A

A

L

L

N

N

A

A

P

P

V

I

K

E

D

K

S

T

Q

D

F

F

G

G

V

I

F

F

R

R

M

M

binding 5-(hexahydro-2-oxo-1h-thieno[3,4-d]imidazol-6-yl)pentanal: L219 (= L213), L224 (= L218), G384 (= G378), F385 (= F379), Y423 (≠ F417), A425 (= A419)
binding ~{S}-[2-[3-[[(2~{R})-4-[[[(2~{S},3~{S},4~{S},5~{S})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyl] 3-methylbut-2-enethioate: R56 (= R51), K119 (= K113), G120 (= G114), A122 (≠ T116), G152 (= G146), G153 (= G147), A154 (= A148), Y155 (≠ F149), F169 (= F163), T195 (= T189), A196 (= A190), G197 (= G191), I463 (≠ V457), Q467 (= Q461)

8jxmC Human 3-methylcrotonyl-coa carboxylase in bccp-h2 state with mcoa
64% identity, 98% coverage: 13:535/535 of query aligns to 18:520/520 of 8jxmC

query
sites
8jxmC

A

A

A

L

F

Y

A

Q

T

E

N

N

R

Y

D

K

A

Q

M

M

Q

K

A

A

Q

L

V

V

D

N

D

Q

L

L

R

H

Q

E

L

R

V

V

D

E

K

H

V

I

R

K

Q

L

G

G

G

G

G

G

A

E

K

K

A

A

Q

R

E

A

R

L

H

H

V

I

S

S

R
|
R

G

G

K

K

L

L

L

L

P

P

R

R

E

E

R

R

L

I

N

D

A

N

L

L

L

I

D

D

P

P

G

G

S

S

P

P

F

F

L

L

E

E

L

L

S

S

Q

Q

L

F

A

A

A

G

H

Y

D

Q

V

L

Y

Y

E

D

H

N

D

E

-

E

V

V

P

P

A

G

A

G

G

G

I

I

I

I

T

T

G

G

I

I

G

G

R

R

V

V

A

S

G

G

Q

V

E

E

C

C

M

M

I

I

F

I

V

A

N

N

D

D

A

A

T

T

V

V

K
|
K

G
|
G

G

G

T

A

Y

Y

Y

Y

P

P

L

V

T

T

V

V

K

K

K

K

Q

Q

G

L

R

R

A

A

Q

Q

T

E

I

I

A

A

A

M

E

Q

N

N

N

R

L

L

P

P

C

C

I

I

Y

Y

L

L

V

V

D

D

S

S

G
|
G

G

G

A
|
A

F
x
Y

L

L

P

P

L

R

Q

Q

D

A

E

D

V

V

F
|
F

P

P

D

D

K

R

D

D

H

H

F

F

G

G

H

R

A

T

F

F

Y

Y

N

N

Q

Q

A

A

R

I

M

M

S

S

A

S

Q

K

G

N

I

I

P

A

Q

Q

I

I

A

A

A

V

V

V

M

M

G

G

S

S

C

C

T
|
T

A
|
A

G

G

G

G

A

A

Y

Y

L

V

P

P

A

A

M

M

A

A

D

D

E

E

S

N

I

I

I

I

V

V

K

R

E

K

Q

Q

G

G

T

T

I

I

F

-

L

-

G

-

G

-

P

-

P

-

L

-

V

-

K

-

A

-

A

-

T

-

G

-

E

-

I

-

V

-

S

-

A

-

E

-

E

-

L

-

G

G

G

G

A

A

D

D

V

L

H

H

C

C

R

R

T

K

S

S

G

G

V

V

T

S

D

D

H

H

Y

W

A

A

N

L

N

D

D

D

H

H

H

H

A

A

L

L

E

H

L

L

V

T

R

R

R

K

S

V

V

V

G

R

R

N

L

L

N

N

R

Y

V

Q

K

K

Q

K

I

L

D

D

I

V

D

T

V

I

R

E

D

P

S

S

I

E

E

E

P

P

A

L

F

F

P

P

V

A

E

D

D

E

I

L

Y

Y

G

G

V

I

I

V

P

G

A

A

D

N

A

L

K

K

Q

R

P

S

Y

F

D

D

V

V

R

R

E

E

V

V

I

I

A

A

R

R

V

I

V

V

D

D

G

G

S

S

E

R

F

F

D

T

E

E

F

F

K

K

A

A

L

F

Y

Y

G

G

E

D

T

T

L

L

V

V

C

T

G

G

F

F

A

A

R

R

I

I

H

F

G

G

Y

Y

P

P

V

V

G

G

I

I

I

V

A

G

N

N

N

N

G

G

I

V

L

L

F

F

A

S

E

E

S

S

S

A

V

K

K

K

G

G

A

T

H

H

F

F

V

V

E

Q

L

L

C

C

A

C

Q

Q

R

R

K

N

I

I

P

P

L

L

V

L

F

F

L

L

Q

Q

N

N

I

I

T
|
T

G
|
G

F
|
F

M

M

V

V

G

G

K

R

Q

E

Y

Y

E

E

H

A

G

E

G

G

I

I

A

A

R

K

H

D

G

G

A

A

K

K

M

M

V

V

H

A

A

A

V

V

A

A

C

C

A

A

E

Q

V

V

P

P

K

K

F

I

T

T

V

L

I

I

I

I

G

G

G

G

S

S

F
x
Y

G
|
G

A
|
A

G

G

N

N

Y

Y

A

G

M

M

C

C

G

G

R

R

A

A

Y

Y

E

S

P

P

R

R

F

F

M

L

F

Y

M

I

W

W

P

P

N

N

A

A

R

R

I

I

S

S

V

V

M
|
M

G

G

G

G

E

E

Q

Q

A

A

A

A

G

N

V

V

L

L

A

A

T

T

V
x
I

K

T

Q

K

E

D

Q
|
Q

L

R

A

A

R

R

E

E

G

G

K

K

A

Q

M

F

T

S

E

S

A

A

E

D

E

E

A

A

A

A

F

L

K

K

Q

E

P

P

T

I

L

I

E

K

L

K

Y

F

E

E

K

E

Q

E

G

G

H

N

P

P

Y

Y

Y

Y

A

S

S

S

A

A

R

R

L

V

W

W

D

D

D

D

G

G

V

I

I

I

D

D

P

P

A

A

D

D

T

T

R

R

M

L

V

V

L

L

G

G

L

L

S

S

L

F

S

S

A

A

A

A

L

L

N

N

A

A

P

P

V

I

K

E

D

K

S

T

Q

D

F

F

G

G

V

I

F

F

R

R

M

M

binding 5-(hexahydro-2-oxo-1h-thieno[3,4-d]imidazol-6-yl)pentanal: T362 (= T377), G363 (= G378), F364 (= F379), Y402 (≠ F417), G403 (= G418), A404 (= A419), M430 (= M445)
binding ~{S}-[2-[3-[[(2~{R})-4-[[[(2~{S},3~{S},4~{S},5~{S})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyl] 3-methylbut-2-enethioate: R56 (= R51), K119 (= K113), G120 (= G114), G152 (= G146), A154 (= A148), Y155 (≠ F149), F163 (= F157), T195 (= T189), A196 (= A190), I442 (≠ V457), Q446 (= Q461)

8f3dA 3-methylcrotonyl-coa carboxylase in filament, beta-subunit centered (see paper)
59% identity, 98% coverage: 2:524/535 of query aligns to 17:566/566 of 8f3dA

query
sites
8f3dA

N

N

V

I

L

L

Q

R

S

L

R

P

I

A

N

N

T

T

R

S

D

D

A

P

A

T

F

Y

A

Q

T

E

N

N

R

M

D

A

A

R

M

M

Q

E

A

G

Q

L

V

V

D

E

D

Q

L

L

R

R

Q

A

L

R

V

V

D

R

K

Y

V

V

R

Q

Q

A

G

G

G

G

-

V

-

V

-

P

-

E

-

E

-

E

G

A

A

A

K

K

A

A

-

G

-

V

-

S

-

I

-

S

-

S

-

I

-

E

-

A

-

D

-

D

-

R

-

V

Q

R

E

K

R

L

H

H

V

L

S

S

R

R

G

G

K

K

L

M

L

L

P

A

R

R

E

D

R

R

L

I

N

E

A

R

L

L

L

I

D

D

P

P

G

G

S

T

P

R

F

F

L

L

E

E

L

L

S

S

Q

Q

L

L

A

A

A

G

H

W

D

D

V

L

Y

Y

E

W

H

D

D

D

V

K

P

K

-

K

-

E

-

Y

-

E

-

R

-

C

-

Y

A

S

A

G

G

G

I

I

I

V

T

T

G

G

I

I

G

G

R

L

V

V

A

N

G

G

Q

V

E

R

C

C

M

M

I

L

F

V

V

A

N

N

D

D

A

A

T

T

V

V

K

K

G

G

G

G

T

T

Y

Y

Y

Y

P

P

L

I

T

T

V

V

K

K

K

K

Q

H

G

L

R

R

A

A

Q

Q

T

K

I

I

A

A

A

E

E

Q

N

N

N

H

L

L

P

P

C

C

I

I

Y

Y

L

L

V

V

D

D

S

S

G

G

G

G

A

A

F

N

L

L

P

S

L

R

Q

Q

D

D

E

D

V

V

F

F

P

P

D

D

K

E

D

Q

H

H

F

F

G

G

H

R

A

I

F

F

Y

Y

N

N

Q

E

A

A

R

Q

M

M

S

S

A

I

Q

K

G

S

I

I

P

S

Q

Q

I

I

A

A

A

V

V

V

M

M

G

G

S

S

C

C

T

T

A

A

G

G

G

G

A

A

Y

Y

L

V

P

P

A

A

M

M

A

A

D

D

E

E

S

N

I

I

I

I

V

V

K

A

E

R

Q

N

G

G

T

T

I

I

F

F

L

L

G

G

G

G

P

P

P

P

L

L

V

V

K

L

A

A

A

A

T

T

G

G

E

E

I

K

V

V

S

S

A

S

E

E

E

E

L

L

G

G

G

G

A

A

D

D

V

V

H

H

C

C

R

R

T

I

S

S

G

G

V

V

T

G

D

D

H

H

Y

Y

A

A

N

T

N

D

D

D

H

L

H

H

A

A

L

L

E

Y

L

L

V

A

R

R

R

R

S

A

V

V

G

A

R

N

L

L

N

N

R

L

V

K

K

E

Q

H

I

N

D

E

I

A

D

R

V

N

R

P

D

T

S

D

I

V

E

K

P

P

A

V

F

P

P

P

V

L

E

Y

D

D

-

P

-

R

-

E

I

L

Y

G

G

G

V

F

I

I

P

P

-

D

-

M

-

L

A

S

D

D

A

V

K

V

Q

K

P

S

Y

F

D

D

V

V

R

R

E

A

V

I

I

I

A

A

R

R

V

I

V

V

D

D

G

G

S

S

E

R

F

F

D

D

E

E

F

F

K

K

A

A

L

L

Y

Y

G

G

E

N

T

T

L

L

V

V

C

C

G

G

F

F

A

A

R

R

I

I

H

E

G

G

Y

M

P

Q

V

V

G

G

I

I

I

I

A

A

N

N

N

Q

G

G

I

I

L

L

F

Y

A

S

E

E

S

S

S

A

V

L

K

K

G

G

A

A

H

H

F

F

V

I

E

G

L

L

C

C

A

T

Q

Q

R

R

K

N

I

V

P

P

L

L

V

L

F

F

L

L

Q

Q

N

N

I

I

T

T

G

G

F
|
F

M

M

V

V

G

G

K

K

Q

K

Y

Y

E

E

H

E

G

G

G

G

I

I

A

A

R

R

H

N

G

G

A

A

K

R

M

L

V

V

H

M

A

A

V

V

A

S

C

S

A

A

E

P

V

V

P

P

K

K

F

V

T

T

V

V

I

L

I

I

G

G

G

G

S

S

F

Y

G

G

A

A

G

G

N

N

Y

Y

A

G

M

M

C

C

G

G

R

R

A

A

Y

F

E

E

P

P

R

R

F

F

M

L

F

F

M

M

W

W

P

P

N

N

A

A

R

R

I

I

S

S

V

V

M

M

G

G

G

G

E

T

Q
|
Q

A

A

A

A

G

T

V

V

L

L

A

T

T

L

V

T

K

N

Q

-

E

-

Q

-

L

-

A

-

R

R

E

N

G

L

K

K

A

N

M

A

T

S

E

E

A

A

E

E

E

I

A

A

A

A

F

F

K

K

Q

D

P

K

T

V

L

K

E

K

L

K

Y

Y

E

E

K

K

Q

E

G

G

H

S

P

C

Y

Y

Y

Y

A

S

S

T

A

A

R

R

L

L

W

W

D

D

D

D

G

G

V

V

I

I

D

A

P

P

A

E

D

D

T

T

R

R

M

V

V

V

L

V

G

A

L

E

S

A

L

L

S

R

A

A

A

T

L

R

N

L

A

A

P

P

binding 5-(hexahydro-2-oxo-1h-thieno[3,4-d]imidazol-6-yl)pentanal: F426 (= F379), Q496 (= Q449)

8rthF Trypanosoma brucei 3-methylcrotonyl-coa carboxylase (see paper)
60% identity, 98% coverage: 2:524/535 of query aligns to 20:542/542 of 8rthF

query
sites
8rthF

N

N

V

V

L

L

Q

H

S

Y

R

P

I

A

N

E

T

S

R

T

D

N

A

P

A

E

F

F

A

K

T

A

N

N

R

T

D

E

A

R

M

M

Q

K

A

A

Q

L

V

T

D

A

D

E

L

L

R

R

Q

R

L

R

V

V

D

Q

K

V

V

I

R

V

Q

D

G

G

-

D

-

S

-

E

G

A

G

D

A

K

K

R

A

A

Q

R

E

D

R

R

H

H

V

I

S

S

R

R

G

G

K

K

L

L

L

L

P

V

R

H

E

Q

R

R

L

I

N

E

A

K

L

L

L

V

D

D

P

P

G

M

S

S

P

P

F

F

L

L

E

E

L

L

S

S

Q

Q

L

L

A

A

A

G

H

G

D

D

V

L

Y

Y

E

P

H

G

D

E

-

A

V

C

P

H

A

R

A

G

G

G

I

I

I

L

T

T

G

G

I

I

G

G

R

V

V

V

A

H

G

G

Q

M

E

R

C

V

M

M

I

I

F

V

V

A

N

N

D

D

A

A

T

T

V

V

K

K

G

G

G

G

T

T

Y

Y

Y

Y

P

P

L

I

T

T

V

V

K

K

K

K

Q

H

G

L

R

R

A

A

Q

Q

T

R

I

I

A

A

A

E

E

E

N

N

N

R

L

L

P

P

C

C

I

I

Y

Y

L

L

V

V

D

D

S

S

G

G

G

G

A

A

F

N

L

L

P

G

L

M

Q

Q

D

G

E

D

V

V

F

F

P

P

D

D

K

E

D

Q

H

H

F

F

G

G

H

R

A

I

F

F

Y

F

N

N

Q

Q

A

A

R

N

M

M

S

S

A

A

Q

K

G

G

I

I

P

A

Q

Q

I

I

A

A

A

T

V

V

M

M

G

G

S

S

C

C

T

T

A

A

G

G

G

G

A

A

Y

Y

L

V

P

P

A

A

M

M

A

S

D

D

E

E

S

S

I

I

I

I

V

V

K

K

E

G

Q

N

G

G

T

T

I

I

F

F

L

L

G

G

G

G

P

P

P

P

L

L

V

V

K

F

A

A

A

A

T

T

G

G

E

E

I

E

V

V

S

T

A

P

E

E

E

E

L

L

G

G

G

G

A

A

D

D

V

V

H

H

C

C

R

R

T

A

S

S

G

G

V

V

T

T

D

D

H

Y

Y

F

A

A

N

T

N

D

D

D

H

L

H

H

A

A

L

L

E

Y

L

L

V

T

R

R

R

R

S

I

V

V

G

A

R

N

L

L

N

N

R

R

V

N

K

D

-

C

Q

E

I

R

D

P

I

C

D

R

V

G

R

R

D

E

S

F

I

T

E

P

P

P

A

L

F

Y

P

D

V

P

E

S

D

E

I

I

Y

G

G

G

V

F

I

I

P

P

-

D

-

M

-

G

A

A

D

D

A

V

K

V

Q

K

P

G

Y

F

D

D

V

V

R

R

E

A

V

V

I

I

A

A

R

R

V

L

V

V

D

D

G

G

S

S

E

E

F

F

D

D

E

E

F

F

K

K

A

K

L

L

Y

Y

G

G

E

D

T

T

L

L

V

V

C

C

G

G

F

F

A

A

R

R

I

F

H

E

G

G

Y

M

P

L

V

V

G

G

I

I

I

V

A

A

N

N

N

N

G

G

I

I

L

L

F

Y

A

S

E

E

S

S

S

A

V

L

K

K

G

G

A

A

H

H

F

F

V

V

E

E

L

L

C

C

A

S

Q

H

R

R

K

N

I

I

P

P

L

L

V

L

F

F

L

L

Q

Q

N

N

I

I

T
|
T

G
|
G

F
|
F

M

M

V

V

G

G

K

K

Q

T

Y

Y

E

E

H

E

G

G

G

G

I

I

A

A

R

K

H

N

G

G

A

A

K

K

M

L

V

V

H

T

A

A

V

V

A

S

C

T

A

T

E

H

V

V

P

P

K

K

F

I

T

T

V

I

I

I

I

I

G

G

G

G

S

S

F

Y

G

G

A

A

G

G

N

N

Y

Y

A

G

M

M

C

C

G

G

R

R

A

A

Y

F

E

G

P

P

R

R

F

F

M

L

F

F

M

M

W

W

P

P

N

N

A

A

R

R

I

I

S

S

V

V

M

M

G

G

G

G

E
x
N

Q
|
Q

A

A

A

A

G

T

V

V

L

L

A

A

T

L

V

T

K

N

Q

S

E

K

Q

-

L

-

A

-

R

-

E

-

G

-

K

-

A

-

M

L

T

R

E

E

A

N

E

E

E

V

A

Q

A

D

F

F

K

K

Q

A

P

K

T

V

L

R

E

S

L

K

Y

Y

E

E

K

Y

Q

E

G

G

H

S

P

C

Y

Y

Y

Y

A

S

S

T

A

A

R

R

L

L

W

W

D

D

D

D

G

G

V

V

I

I

D

A

P

P

A

E

D

D

T

T

R

R

M

A

V

V

L

V

G

V

L

Q

S

A

L

L

S

L

A

S

A

T

L

L

N

S

A

A

P

P

binding 5-(hexahydro-2-oxo-1h-thieno[3,4-d]imidazol-6-yl)pentanal: T403 (= T377), G404 (= G378), F405 (= F379), N474 (≠ E448), Q475 (= Q449)

1vrgA Crystal structure of propionyl-coa carboxylase, beta subunit (tm0716) from thermotoga maritima at 2.30 a resolution
35% identity, 92% coverage: 21:510/535 of query aligns to 2:488/515 of 1vrgA

query
sites
1vrgA

A

S

M

L

Q

R

A

D

Q

K

V

I

D

E

D

E

L

L

R

K

Q

K

L

I

V

E

D

K

K

E

V

I

R

E

Q

Q

G

G

G

G

G

G

A

P

K

E

A

K

Q

V

E

E

R

K

H

Q

V

H

S

R

R

A

G

G

K

K

L

L

L

T

P

A

R

W

E

E

R

R

L

L

N

E

A

L

L

L

L

L

D

D

P

P

G

G

S

T

P

-

F

F

L

V

E

E

L

I

S

D

Q

K

L

F

A

V

A

E

H

H

-

R

D

N

V

T

Y

Y

-

F

-

G

-

L

-

D

E

K

H

V

D

K

V

L

P

P

A

R

A

D

G

G

I

V

I

I

T

T

G

G

I

V

G

G

R

E

V

I

A

N

G

G

Q

R

E

K

C

V

M

A

I

V

F

F

V

S

N

Q

D

D

A

F

T

T

V

V

K

M

G

G

G

G

T

S

Y

L

Y

G

P

E

L

M

T

H

V

A

K

K

K

K

Q

I

G

V

R

K

A

L

Q

L

T

D

I

L

A

A

A

L

E

K

N

M

N

G

L

I

P

P

C

V

I

I

Y

G

L

I

V

N

D

D

S

S

G

G

G

G

A
|
A

F

R

L

I

P

-

L

-

Q

-

D

Q

E

E

V

G

F

V

P

D

D

A

K

L

D

A

H

G

F

Y

G

G

H

E

A

I

F

F

Y

L

N

R

Q

N

A

T

R

-

M

L

S

A

A

S

Q

G

G

V

I

V

P

P

Q

Q

I

I

A

T

A

V

V

I

M

A

G

G

S

P

C

C

T

A

A
x
G

G
|
G

G

A

A

V

Y

Y

L

S

P

P

A

A

M

L

A

T

D

D

E

F

S

I

I

V

I

M

V

V

K

D

E

Q

Q

T

G

A

T

R

I

M

F

F

L

I

G

T

G
|
G

P

P

P
x
N

L
x
V

V

I

K

K

A

A

A

V

T

T

G

G

E

E

I

E

V

I

S

S

A

Q

E

E

E

D

L

L

G

G

G

G

A

A

D

M

V

V

H

H

C

N

R

Q

T

K

S

S

G

G

V

N

T

A

D

H

H

F

Y

L

A

A

N

D

N

N

D

D

H

E

H

K

A

A

L

M

E

S

L

L

V

V

R

R

R

T

S

L

V

L

G

S

R

Y

L

L

N

P

R

S

V

N

K

N

Q

A

I

E

D

E

I

P

D

P

V

V

R

E

D

D

S

P

I

D

E

T

P

S

A

L

F

E

P

T

V

P

E

E

D

D

I

I

Y

L

G

D

V

I

I

L

P

P

A

D

D

N

A

P

K

N

Q

K

P

G

Y

Y

D

D

V

V

R

R

E

D

V

V

I

I

A

K

R

R

V

V

V

V

D

D

G

H

S

G

E

E

F

F

D

F

E

E

F

V

K

Q

A

P

L

Y

Y

F

G

A

E

K

T

N

L

I

V

V

C

I

G

G

F

F

A

A

R

R

I

I

H

Q

G

G

Y

K

P

T

V

V

G

G

I

I

I

V

A

A

N

N

N

Q

-

P

-

S

-

V

-

L

-

A

G

G

I

V

L

L

F

D

A

I

E

D

S

S

S

S

V

D

K

K

G

A

A

A

H

R

F

F

V

I

E

R

L

F

C

L

A

D

Q

A

R

F

K

N

I

I

P

P

L

I

V

L

F

T

L

F

Q

V

N

D

I

T

T

P

G

G

F
x
Y

M

L

V

P

G

G

K

V

Q

A

Y

Q

E

E

H

H

G

G

G

G

I

I

A

I

R

R

H

H

G

G

A

A

K

K

M

L

V

L

H

Y

A

A

V

Y

A

S

C

E

A

A

E

T

V

V

P

P

K

K

F

I

T

T

V

V

I

I

I

L

G

R

G

K

S

A

F

Y

G

G

A
x
G

G
x
A

N

Y

Y

I

A

A

M

M

C

G

G

S

R

K

A

H

Y

L

E

G

P

A

R

D

F

M

M

V

F

L

M

A

W

W

P

P

N

S

A

A

R

E

I

I

S

A

V

V

M

M

G

G

G

P

E

E

Q

G

A

A

A

A

G

N

V

I

L

I

A

-

T

-

V

-

K

-

Q

-

E

-

Q

-

L

F

A

K

R

R

E

E

G

I

K

E

A

A

M

S

T

S

E

N

A

P

E

E

E

E

A

T

A

-

F

-

K

R

Q

R

P

K

T

L

L

I

E

E

L

E

Y

Y

E

K

K

Q

Q

Q

-

F

G

A

H

N

P

P

Y

Y

Y

I

A

A

S

A

A

S

R

R

L

G

W

Y

D

V

D

D

G

M

V

V

I

I

D

D

P

P

A

R

D

E

T

T

R

R

active site: A133 (= A148), G171 (≠ A190), G172 (= G191), Y365 (≠ F379), G405 (≠ A419), A406 (≠ G420)
binding bicarbonate ion: G196 (= G215), N198 (≠ P217), V199 (≠ L218)

1on3E Transcarboxylase 12s crystal structure: hexamer assembly and substrate binding to a multienzyme core (with methylmalonyl-coenzyme a and methylmalonic acid bound) (see paper)
36% identity, 93% coverage: 22:517/535 of query aligns to 9:500/520 of 1on3E

query
sites
1on3E

M

M

Q

E

A

G

Q

R

V

V

D

E

D

Q

L

L

R

A

Q

E

L

Q

V

R

D

Q

K

V

V

I

R

E

Q

A

G

G

G

G

G

G

A

E

K

R

A
x
R

Q

V

E

E

R

K

H

Q

V

H

S

S

R

Q

G

G

K

K

L

Q

L

T

P

A

R

R

E

E

R

R

L

L

N

N

A

N

L

L

L

L

D

D

P

P

G

H

S

S

P

-

F

F

L

D

E

E

L

V

S

G

Q

A

L

F

A

R

A

K

H

H

-

R

-

T

D

T

V

L

Y

F

E

G

H

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active site: A139 (= A148), G177 (≠ A190), G178 (= G191), F370 (= F379), G410 (≠ A419), S411 (≠ G420)
binding methylmalonyl-coenzyme a: R31 (≠ A44), F101 (≠ A110), G105 (= G114), S107 (≠ T116), G137 (= G146), A139 (= A148), I141 (≠ L150), G177 (≠ A190), G178 (= G191), G409 (= G418), V435 (= V444)

1on3C Transcarboxylase 12s crystal structure: hexamer assembly and substrate binding to a multienzyme core (with methylmalonyl-coenzyme a and methylmalonic acid bound) (see paper)
36% identity, 93% coverage: 22:517/535 of query aligns to 5:490/510 of 1on3C

query
sites
1on3C

M

M

Q

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