SitesBLAST
Comparing WP_084275369.1 NCBI__GCF_900176045.1:WP_084275369.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
7ahhC Opua inhibited inward-facing, sbd docked (see paper)
39% identity, 86% coverage: 3:210/243 of query aligns to 2:246/382 of 7ahhC
Sites not aligning to the query:
- binding (2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide: 275, 297, 298
7aheC Opua inhibited inward facing (see paper)
39% identity, 86% coverage: 3:210/243 of query aligns to 2:246/382 of 7aheC
Sites not aligning to the query:
- binding (2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide: 275, 297, 298
7ahdC Opua (e190q) occluded (see paper)
38% identity, 86% coverage: 2:210/243 of query aligns to 1:246/260 of 7ahdC
- binding adenosine-5'-triphosphate: F12 (= F13), T39 (vs. gap), S61 (= S38), G62 (= G39), G64 (= G41), K65 (= K42), S66 (≠ T43), T67 (= T44), Q111 (= Q72), K161 (= K123), Q162 (≠ E124), S164 (= S126), G166 (= G128), M167 (= M129), Q188 (≠ E150), H221 (= H183)
P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
37% identity, 86% coverage: 1:208/243 of query aligns to 1:215/369 of P19566
- L86 (= L76) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- P160 (= P151) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- D165 (= D156) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
Sites not aligning to the query:
- 306 E→K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.
P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
36% identity, 86% coverage: 1:208/243 of query aligns to 1:215/371 of P68187
- A85 (≠ R75) mutation to M: Suppressor of EAA loop mutations in MalFG.
- K106 (≠ S96) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V114 (≠ A104) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V117 (≠ I107) mutation to M: Suppressor of EAA loop mutations in MalFG.
- E119 (≠ L109) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- A124 (≠ E114) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G137 (= G128) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
- D158 (= D149) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
Sites not aligning to the query:
- 228 R→C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 267 W→G: Normal maltose transport but constitutive mal gene expression.
- 278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
- 322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 340 G→A: Maltose transport is affected but retains ability to interact with MalT.
- 346 G→S: Normal maltose transport but constitutive mal gene expression.
- 355 F→Y: Maltose transport is affected but retains ability to interact with MalT.
8wm7D Cryo-em structure of cyanobacterial nitrate/nitrite transporter nrtbcd in complex with signalling protein pii (see paper)
38% identity, 83% coverage: 14:214/243 of query aligns to 19:226/257 of 8wm7D
Sites not aligning to the query:
2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
36% identity, 85% coverage: 2:208/243 of query aligns to 1:214/374 of 2awnB
3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
36% identity, 85% coverage: 2:208/243 of query aligns to 1:214/371 of 3puyA
- binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ F13), S37 (= S38), G38 (= G39), C39 (≠ G40), G40 (= G41), K41 (= K42), S42 (≠ T43), T43 (= T44), Q81 (= Q72), R128 (≠ K120), A132 (≠ E124), S134 (= S126), G136 (= G128), Q137 (≠ M129), E158 (= E150), H191 (= H183)
- binding magnesium ion: S42 (≠ T43), Q81 (= Q72)
3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
36% identity, 85% coverage: 2:208/243 of query aligns to 1:214/371 of 3puxA
- binding adenosine-5'-diphosphate: W12 (≠ F13), G38 (= G39), C39 (≠ G40), G40 (= G41), K41 (= K42), S42 (≠ T43), T43 (= T44), R128 (≠ K120), S134 (= S126), Q137 (≠ M129)
- binding beryllium trifluoride ion: S37 (= S38), G38 (= G39), K41 (= K42), Q81 (= Q72), S134 (= S126), G136 (= G128), H191 (= H183)
- binding magnesium ion: S42 (≠ T43), Q81 (= Q72)
3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
36% identity, 85% coverage: 2:208/243 of query aligns to 1:214/371 of 3puwA
- binding adenosine-5'-diphosphate: W12 (≠ F13), V17 (≠ I18), G38 (= G39), C39 (≠ G40), G40 (= G41), K41 (= K42), S42 (≠ T43), T43 (= T44), R128 (≠ K120), A132 (≠ E124), S134 (= S126), Q137 (≠ M129)
- binding tetrafluoroaluminate ion: S37 (= S38), G38 (= G39), K41 (= K42), Q81 (= Q72), S134 (= S126), G135 (= G127), G136 (= G128), E158 (= E150), H191 (= H183)
- binding magnesium ion: S42 (≠ T43), Q81 (= Q72)
3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
36% identity, 85% coverage: 2:208/243 of query aligns to 1:214/371 of 3puvA
- binding adenosine-5'-diphosphate: W12 (≠ F13), V17 (≠ I18), G38 (= G39), C39 (≠ G40), G40 (= G41), K41 (= K42), S42 (≠ T43), T43 (= T44), R128 (≠ K120), A132 (≠ E124), S134 (= S126), Q137 (≠ M129)
- binding magnesium ion: S42 (≠ T43), Q81 (= Q72)
8w9mD Cryo-em structure of the cyanobacterial nitrate transporter nrtbcd in complex with atp (see paper)
38% identity, 83% coverage: 14:214/243 of query aligns to 17:224/256 of 8w9mD
- binding adenosine-5'-triphosphate: H40 (≠ P37), S41 (= S38), G42 (= G39), G44 (= G41), K45 (= K42), S46 (≠ T43), T47 (= T44), Q82 (= Q72), Q135 (≠ E124), S137 (= S126), G139 (= G128), M140 (= M129), H194 (= H183)
- binding magnesium ion: S46 (≠ T43), Q82 (= Q72)
Sites not aligning to the query:
2d62A Crystal structure of multiple sugar binding transport atp- binding protein
37% identity, 81% coverage: 1:198/243 of query aligns to 4:216/375 of 2d62A
P69874 Spermidine/putrescine import ATP-binding protein PotA; EC 7.6.2.11 from Escherichia coli (strain K12) (see 3 papers)
41% identity, 80% coverage: 12:205/243 of query aligns to 26:226/378 of P69874
- C26 (≠ S12) mutation to A: Lower ATPase activity and transport efficiency.
- F27 (= F13) mutation to L: Lower ATPase activity and transport efficiency.
- F45 (≠ V31) mutation to L: Lower ATPase activity and transport efficiency.
- C54 (≠ G40) mutation to T: Loss of ATPase activity and transport.
- L60 (= L46) mutation to F: Lower ATPase activity and transport efficiency.
- L76 (= L53) mutation to P: Lower ATPase activity and transport efficiency.
- V135 (≠ D112) mutation to M: Loss of ATPase activity and transport.
- D172 (= D149) mutation to N: Loss of ATPase activity and transport.
Sites not aligning to the query:
- 276 C→A: Lower ATPase activity and transport efficiency.
- 297 mutation E->K,D: Lower ATPase activity and transport efficiency.; E→Q: Loss of ATPase activity and transport.
1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
36% identity, 84% coverage: 4:208/243 of query aligns to 1:212/367 of 1q12A
- binding adenosine-5'-triphosphate: W10 (≠ F13), S35 (= S38), G36 (= G39), C37 (≠ G40), G38 (= G41), K39 (= K42), S40 (≠ T43), T41 (= T44), R126 (≠ K120), A130 (≠ E124), S132 (= S126), G134 (= G128), Q135 (≠ M129)
8wm7C Cryo-em structure of cyanobacterial nitrate/nitrite transporter nrtbcd in complex with signalling protein pii (see paper)
39% identity, 84% coverage: 14:217/243 of query aligns to 18:228/658 of 8wm7C
Sites not aligning to the query:
8w9mC Cryo-em structure of the cyanobacterial nitrate transporter nrtbcd in complex with atp (see paper)
39% identity, 84% coverage: 14:217/243 of query aligns to 18:228/256 of 8w9mC
- binding adenosine-5'-triphosphate: Y20 (≠ K16), S42 (= S38), G43 (= G39), G45 (= G41), K46 (= K42), S47 (≠ T43), T48 (= T44), Q83 (= Q72), K132 (= K120), E136 (= E124), S138 (= S126), G140 (= G128), H195 (= H183)
- binding magnesium ion: S47 (≠ T43), Q83 (= Q72)
Sites not aligning to the query:
2awnC Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
39% identity, 79% coverage: 17:208/243 of query aligns to 9:184/344 of 2awnC
1vciA Crystal structure of the atp-binding cassette of multisugar transporter from pyrococcus horikoshii ot3 complexed with atp (see paper)
37% identity, 82% coverage: 1:199/243 of query aligns to 4:203/353 of 1vciA
1g291 Malk (see paper)
36% identity, 82% coverage: 1:199/243 of query aligns to 1:214/372 of 1g291
- binding magnesium ion: D69 (vs. gap), E71 (vs. gap), K72 (≠ E61), K79 (= K65), D80 (≠ T66)
- binding pyrophosphate 2-: S38 (= S38), G39 (= G39), C40 (≠ G40), G41 (= G41), K42 (= K42), T43 (= T43), T44 (= T44)
Sites not aligning to the query:
Query Sequence
>WP_084275369.1 NCBI__GCF_900176045.1:WP_084275369.1
MARLKVEHLTFSFGYKTILEDISFEIHEGEVVSVVGPSGGGKTTLLRLCAGLLDRQEGTI
ENSFKTQSIAFQDPRLLPWKNVLDNIAFGLKAQGVSKKERIKRAQEIALQFDLEEDDFEK
FPKELSGGMSQRVSFARALVTQPELLFLDEPFSALDIGLKRELQNHLIELITKKEITIFF
ITHDLMEAVRLSDKIFVLEPDPGRIVKTFTLDIPQSKRDDSYVYSETAKLLKDPYIIETF
ELE
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory