SitesBLAST
Comparing WP_084517243.1 NCBI__GCF_000422285.1:WP_084517243.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
1aorA Structure of a hyperthermophilic tungstopterin enzyme, aldehyde ferredoxin oxidoreductase (see paper)
34% identity, 54% coverage: 551:1195/1201 of query aligns to 3:601/605 of 1aorA
- binding fe (iii) ion: E332 (≠ D917), H383 (≠ S967)
- binding tungstopterin cofactor: R76 (= R624), A92 (= A640), N93 (= N641), G95 (= G643), R182 (= R730), A183 (≠ L731), G185 (≠ A733), R186 (= R734), E311 (= E896), E313 (= E898), D338 (≠ N923), D343 (= D928), T344 (= T929), R444 (≠ T1030), H448 (= H1034), I449 (≠ T1035), K450 (≠ I1036), D489 (≠ N1083), C494 (= C1088), L495 (= L1089), F496 (= F1090)
- binding iron/sulfur cluster: R76 (= R624), C288 (= C869), C291 (= C872), I293 (≠ L874), C295 (= C876), C494 (= C1088), F496 (= F1090)
8c0zB Cryoem structure of a tungsten-containing aldehyde oxidoreductase from aromatoleum aromaticum (see paper)
30% identity, 54% coverage: 551:1195/1201 of query aligns to 2:615/616 of 8c0zB
- binding magnesium ion: N92 (= N641), A182 (≠ L731)
- binding iron/sulfur cluster: R75 (= R624), C295 (= C869), C298 (= C872), C302 (= C876), C509 (= C1088)
- binding tungsten cofactor: R75 (= R624), S91 (≠ A640), N92 (= N641), G94 (= G643), R181 (= R730), A182 (≠ L731), A183 (= A732), G184 (≠ A733), E329 (= E896), E331 (= E898), N356 (= N923), P362 (≠ T929), L465 (≠ T1035), D504 (≠ N1083), V510 (≠ L1089), F511 (= F1090)
8c0zA Cryoem structure of a tungsten-containing aldehyde oxidoreductase from aromatoleum aromaticum (see paper)
30% identity, 54% coverage: 551:1195/1201 of query aligns to 2:615/616 of 8c0zA
- binding iron/sulfur cluster: R181 (= R730), C295 (= C869), C298 (= C872), C302 (= C876), C509 (= C1088)
- binding tungsten cofactor: R75 (= R624), N92 (= N641), G94 (= G643), R181 (= R730), A183 (= A732), G184 (≠ A733), R185 (= R734), E329 (= E896), E331 (= E898), N356 (= N923), P362 (≠ T929), L465 (≠ T1035), D504 (≠ N1083), V510 (≠ L1089), F511 (= F1090)
1b4nA Formaldehyde ferredoxin oxidoreductase from pyrococcus furiosus, complexed with glutarate (see paper)
30% identity, 54% coverage: 551:1199/1201 of query aligns to 3:601/611 of 1b4nA
- binding calcium ion: G179 (= G729), E304 (≠ K894), D306 (≠ E896)
- binding glutaric acid: Y307 (= Y897), E308 (= E898), Y416 (≠ H1013), H437 (= H1034), R473 (= R1073), R484 (vs. gap)
- binding tungstopterin cofactor: K75 (≠ R624), G91 (≠ A640), N92 (= N641), L93 (≠ V642), G94 (= G643), R180 (= R730), A181 (≠ L731), A182 (= A732), G183 (≠ A733), R184 (= R734), D306 (≠ E896), E308 (= E898), N309 (≠ T899), D333 (≠ N923), M337 (≠ I927), D338 (= D928), T339 (= T929), H436 (≠ R1033), H437 (= H1034), K438 (≠ T1035), F477 (= F1090), E478 (≠ G1091), A482 (≠ S1095), C483 (≠ P1096), R484 (vs. gap), L485 (= L1097)
- binding iron/sulfur cluster: K75 (≠ R624), G283 (≠ A868), C284 (= C869), C287 (= C872), M289 (≠ L874), C291 (= C876), C483 (≠ P1096), L485 (= L1097)
1b25A Formaldehyde ferredoxin oxidoreductase from pyrococcus furiosus (see paper)
30% identity, 54% coverage: 551:1199/1201 of query aligns to 3:601/611 of 1b25A
- binding tungstopterin: K75 (≠ R624), G91 (≠ A640), N92 (= N641), L93 (≠ V642), G94 (= G643), G179 (= G729), R180 (= R730), A181 (≠ L731), G183 (≠ A733), R184 (= R734), T240 (= T820), E304 (≠ K894), D306 (≠ E896), Y307 (= Y897), E308 (= E898), N309 (≠ T899), D333 (≠ N923), D338 (= D928), T339 (= T929), I340 (= I930), H436 (≠ R1033), H437 (= H1034), K438 (≠ T1035), E478 (≠ G1091), A482 (≠ S1095), C483 (≠ P1096), R484 (vs. gap), L485 (= L1097)
- binding iron/sulfur cluster: K75 (≠ R624), R180 (= R730), C284 (= C869), C287 (= C872), M289 (≠ L874), C291 (= C876), C483 (≠ P1096)
4z3yA Active site complex bambc of benzoyl coenzyme a reductase in complex with benzoyl-coa (see paper)
28% identity, 54% coverage: 553:1195/1201 of query aligns to 6:637/653 of 4z3yA
- binding benzoyl coenzyme A: P249 (= P805), W259 (≠ Y818), F323 (≠ G892), L436 (≠ M1012), S439 (= S1015), M440 (≠ R1016), Y445 (= Y1021), T458 (vs. gap), E461 (= E1028), Q466 (vs. gap), R500 (= R1064), S504 (≠ R1068)
- binding magnesium ion: M94 (≠ N641), S183 (≠ L731)
- binding iron/sulfur cluster: R77 (= R624), R181 (≠ G729), C299 (= C869), C302 (= C872), M304 (≠ L874), K305 (≠ G875), C306 (= C876), C534 (≠ G1084)
4z3wC Active site complex bambc of benzoyl coenzyme a reductase in complex with 1,5 dienoyl-coa (see paper)
28% identity, 54% coverage: 553:1195/1201 of query aligns to 6:637/653 of 4z3wC
- binding 1,5 Dienoyl-CoA: P249 (= P805), W259 (≠ Y818), H260 (≠ G819), F323 (≠ G892), L436 (≠ M1012), L438 (≠ D1014), S439 (= S1015), M440 (≠ R1016), Y445 (= Y1021), T458 (vs. gap), E461 (= E1028), Q466 (vs. gap), P499 (≠ T1063), R500 (= R1064), S504 (≠ R1068)
- binding magnesium ion: M94 (≠ N641), S183 (≠ L731)
- binding iron/sulfur cluster: R77 (= R624), R181 (≠ G729), C299 (= C869), C302 (= C872), M304 (≠ L874), C306 (= C876), C534 (≠ G1084)
4z3zA Active site complex bambc of benzoyl coenzyme a reductase in complex with zinc (see paper)
28% identity, 54% coverage: 553:1195/1201 of query aligns to 6:637/652 of 4z3zA
- binding magnesium ion: M94 (≠ N641), S183 (≠ L731)
- binding iron/sulfur cluster: R77 (= R624), R181 (≠ G729), C299 (= C869), C302 (= C872), M304 (≠ L874), C306 (= C876), C534 (≠ G1084)
- binding zinc ion: E251 (≠ N807), H255 (≠ L811), E257 (= E813), H260 (≠ G819)
6x1oC Wor5 from pyrococcus furiosus, as crystallized (see paper)
26% identity, 54% coverage: 552:1195/1201 of query aligns to 4:608/624 of 6x1oC
- binding magnesium ion: M194 (≠ G729), D323 (≠ H893), D326 (≠ E896)
- binding phosphate ion: R249 (= R816), Y296 (= Y867)
- binding tungstopterin cofactor: K77 (≠ R624), S93 (≠ A640), V94 (≠ N641), L95 (≠ V642), S96 (≠ G643), H195 (≠ R730), A196 (≠ L731), A197 (= A732), G198 (≠ A733), Y199 (≠ R734), D326 (≠ E896), E328 (= E898), D353 (≠ N923), D358 (= D928), G359 (≠ T929), I360 (= I930), H469 (= H1034), T470 (= T1035), Y489 (≠ I1082), D490 (≠ N1083), I494 (≠ L1087), C495 (= C1088), N496 (≠ L1089), F497 (= F1090)
- binding iron/sulfur cluster: S96 (≠ G643), D299 (= D870), C302 (= C872), C306 (= C876), C495 (= C1088), F497 (= F1090), V498 (≠ G1091)
- binding (1R)-1-hydroxybutane-1-sulfonic acid: T253 (= T820), Y327 (= Y897), E328 (= E898), H446 (= H1013), H469 (= H1034)
1coyA Crystal structure of cholesterol oxidase complexed with a steroid substrate. Implications for fad dependent alcohol oxidases (see paper)
27% identity, 43% coverage: 12:524/1201 of query aligns to 12:496/501 of 1coyA
- active site: E358 (≠ G365), H442 (= H465), N480 (= N508)
- binding 3-beta-hydroxy-5-androsten-17-one: V74 (≠ R63), F80 (≠ I69), G117 (≠ A94), P341 (≠ T347), Y441 (≠ A464)
- binding flavin-adenine dinucleotide: G15 (= G15), G17 (= G17), Y18 (≠ F18), E38 (= E38), M39 (≠ K39), Y104 (vs. gap), R107 (≠ T84), G111 (= G88), G112 (= G89), V115 (= V92), N116 (≠ Y93), G117 (≠ A94), M119 (≠ N96), V247 (≠ A228), A286 (≠ G265), G287 (= G266), Y441 (≠ A464), H442 (= H465), G470 (= G498), N480 (= N508), P481 (= P509), F482 (≠ S510)
P12676 Cholesterol oxidase; CHOD; Cholesterol isomerase; EC 1.1.3.6; EC 5.3.3.1 from Streptomyces sp. (strain SA-COO) (see paper)
47% identity, 5% coverage: 465:528/1201 of query aligns to 484:542/546 of P12676
- H484 (= H465) mutation H->D,E: Mutants do not catalyze oxidation (>100000-fold reduced), but do catalyze isomerization, 10000 times slower than wild type.; mutation to K: Mutant is inactive in both oxidation (>100000-fold reduced) and isomerization assays (>10000-fold reduced).; mutation to N: Mutant activity is 4400-fold lower than wild type for oxidation, and is 30-fold lower than wild type for isomerization.; mutation to Q: Mutant activity is 120-fold lower than wild type for oxidation, and the same as wild type for isomerization.
Sites not aligning to the query:
- 398 E→Q: Mutant does not catalyze isomerization (>10000-fold reduced), and oxidation activity is 30-fold lower than wild type.
4u2sA Cholesterol oxidase in the reduced state complexed with isopropanol (see paper)
47% identity, 5% coverage: 465:528/1201 of query aligns to 439:497/498 of 4u2sA
Sites not aligning to the query:
- active site: 353
- binding dihydroflavine-adenine dinucleotide: 8, 11, 12, 31, 32, 33, 99, 102, 106, 107, 111, 112, 114, 210, 241, 242, 280, 281, 282, 438
4u2lA Dithionite reduced cholesterol in complex with sulfite (see paper)
47% identity, 5% coverage: 465:528/1201 of query aligns to 439:497/498 of 4u2lA
- active site: H439 (= H465), N477 (= N508)
- binding (s)-10-((2s,3s,4r)-5-((s)-((s)-(((2r,3s,4r,5r)-5-(6-amino-9h-purin-9-yl)-3,4-dihydroxy-tetrahydrofuran-2-yl)methoxy)(hydroxy)phosphoryloxy)(hydroxy)phosphoryloxy)-2,3,4-trihydroxypentyl)-7,8-dimethyl-2,4-dioxo-2,3,4,4a-tetrahydrobenzo[g]pteridine-5(10h)-sulfonic acid: H439 (= H465), D466 (= D497), G467 (= G498), N477 (= N508), P478 (= P509), F479 (≠ S510)
Sites not aligning to the query:
- active site: 353
- binding (s)-10-((2s,3s,4r)-5-((s)-((s)-(((2r,3s,4r,5r)-5-(6-amino-9h-purin-9-yl)-3,4-dihydroxy-tetrahydrofuran-2-yl)methoxy)(hydroxy)phosphoryloxy)(hydroxy)phosphoryloxy)-2,3,4-trihydroxypentyl)-7,8-dimethyl-2,4-dioxo-2,3,4,4a-tetrahydrobenzo[g]pteridine-5(10h)-sulfonic acid: 8, 11, 12, 31, 32, 33, 99, 102, 106, 107, 110, 111, 112, 114, 210, 241, 242, 281, 282, 438
1n1pA Atomic resolution structure of cholesterol oxidase @ ph 7.4 (streptomyces sp. Sa-coo) (see paper)
47% identity, 5% coverage: 465:528/1201 of query aligns to 439:497/498 of 1n1pA
Sites not aligning to the query:
- active site: 353
- binding flavin-adenine dinucleotide: 8, 9, 11, 12, 31, 32, 33, 99, 102, 106, 107, 111, 112, 114, 241, 242, 280, 281, 282, 438
- binding manganese (ii) ion: 323, 416, 498
1mxtA Atomic resolution structure of cholesterol oxidase (streptomyces sp. Sa-coo) (see paper)
47% identity, 5% coverage: 465:528/1201 of query aligns to 439:497/498 of 1mxtA
Sites not aligning to the query:
- active site: 353
- binding flavin-n7 protonated-adenine dinucleotide: 8, 9, 11, 12, 31, 32, 33, 99, 102, 106, 107, 111, 112, 114, 241, 242, 281, 282, 438
- binding oxygen molecule: 114, 353
1b4vA Cholesterol oxidase from streptomyces (see paper)
47% identity, 5% coverage: 465:528/1201 of query aligns to 439:497/498 of 1b4vA
Sites not aligning to the query:
- active site: 353
- binding flavin-adenine dinucleotide: 8, 9, 11, 12, 31, 32, 33, 99, 102, 106, 107, 111, 112, 114, 210, 241, 242, 281, 282, 438
3b3rA Crystal structure of streptomyces cholesterol oxidase h447q/e361q mutant bound to glycerol (0.98a) (see paper)
47% identity, 5% coverage: 467:528/1201 of query aligns to 446:502/503 of 3b3rA
Sites not aligning to the query:
- active site: 358, 444
- binding flavin-n7 protonated-adenine dinucleotide: 13, 16, 17, 36, 37, 38, 104, 107, 111, 112, 116, 117, 119, 246, 247, 286, 287, 443
- binding glycerol: 41, 117, 234, 341, 443
B5WWZ8 Long-chain-alcohol oxidase FAO1; Long-chain fatty alcohol oxidase 1; EC 1.1.3.20 from Lotus japonicus (Lotus corniculatus var. japonicus) (see paper)
38% identity, 5% coverage: 459:524/1201 of query aligns to 675:740/749 of B5WWZ8
Sites not aligning to the query:
- 396 C→G: Loss of activity.
Q01738 Cellobiose dehydrogenase; CDH; Cellobiose-quinone oxidoreductase; EC 1.1.99.18 from Phanerodontia chrysosporium (White-rot fungus) (Sporotrichum pruinosum) (see paper)
27% identity, 12% coverage: 7:155/1201 of query aligns to 233:391/773 of Q01738
Sites not aligning to the query:
- 19 modified: Pyrrolidone carboxylic acid
1kdgB Crystal structure of the flavin domain of cellobiose dehydrogenase (see paper)
27% identity, 12% coverage: 7:155/1201 of query aligns to 6:164/546 of 1kdgB
- binding 6-hydroxy-flavin-adenine dinucleotide: V13 (≠ I14), G14 (= G15), P17 (≠ F18), E37 (= E38), R38 (≠ K39), G90 (= G83), C91 (≠ T84), G95 (= G88), G96 (= G89), N100 (≠ Y93), G101 (≠ A94), L103 (≠ N96)
- binding alpha-D-mannopyranose: T6 (≠ S7), K32 (≠ R33)
Sites not aligning to the query:
- active site: 400, 480, 523
- binding 6-hydroxy-flavin-adenine dinucleotide: 218, 219, 257, 258, 479, 480, 512, 513, 523, 525, 528
- binding alpha-D-mannopyranose: 4, 5
Query Sequence
>WP_084517243.1 NCBI__GCF_000422285.1:WP_084517243.1
MRQDPVSEYDYVVIGSGFGGSVSALRLAQKGYRVAVLEKGRRYRTEDFPQTNWNLRKYLW
LPRLGLYGIQVLTLLRNVFILSGTGVGGGSLVYANNLLVPPDEVFRKPEWGPGDWRAKLA
PYYAKARAMLGAVRCPQVGQADRLLAEVGRELRGEDTFHVNDVGVFFGPPGKTVPDPYFG
GEGPPRTGCTHCGACMIGCPVGGKNTLDKNYLHLAERAGARIFPETEATAIRPRAGGGYT
VFTRQPTGLRHPRRTFTARGVVLSGGVLGTVKLLQKCRDAGFLPGLSPRLGEGVRTNSEA
LLGVKANDPGADYSDQIAITSGIYPDPATHIEVVRYNRGSDAMALLTTLLTDGGGRMPRA
LRFLGIALRRPGAFLRSLWPFGWAARTSILLVMQTDENQIRLDFKPRWWRLGLKSLNSSL
APGARKVPSYIPIANEVARRMAEKIGGEPVSVWTEVLLDVSTTAHILGGCVMGAGAEKGV
VDHAGRVHGYPGLWVADGSVVPANLGVNPSLTITALAEYFMDQVPEKEKPSVAPGPPDGR
ATGTRRRPAGGYTGRVLVVDLSRRSTEVYEPADEDYRRYLSGYGLGAAFIMERQRPGVAP
FSNGSYLGFCSGLLGGTGALFGGRLMVVGKSPLTGGWGDANVGGSLSRALKRCGYDAVFF
TGRADPPAWVHLTDKGVEIRDASELWGKDSVATEHFIRERLEAPRARVACIGPAGERLSL
MAGIATDGGRLAARSGLGALMGWKRLKALSFDGRKRVSVARPAEIKRINQRFLKRFRKSG
LQDRLLLRLTNPFSRFLALTGLPAPANPALLRELLRRYGTSGFTLYSTMIGDMPIRNWSG
VGHLDYGFSRSAKLSDESVLRHQVRRYACDACPLGCGGTIRIEAGRYAGETGHKPEYETL
AAFGGLLLQDDLAAIIDMNEACNRAGIDTISAGAAIAFAVECFEAGLIDENTTGGLRLGW
GRTREISTLLRQIIEREGFGDILADGVKQAALRLGKGTESLAVHAGGQELPMHDSRLDPG
YAIAYQCEPTPGRHTISSYLYADLFGVDRRFPHARRRIRTARTRTARRVERYRAGSIYMQ
LINGAGLCLFGALTSPLPVVDYLNAATGWDLPADAYFDTGERILSLRKAFNAREGIKPED
QRLNPRALGHPPLQRGPLKGRRLDMDRLERCFYETMGWDLETGGPTRETAVRLGLEKICG
V
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory