SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_084527986.1 NCBI__GCF_001653335.1:WP_084527986.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

5mh6A D-2-hydroxyacid dehydrogenases (d2-hdh) from haloferax mediterranei in complex with 2-ketohexanoic acid and NAD+ (1.35 a resolution)
37% identity, 77% coverage: 63:284/290 of query aligns to 60:292/306 of 5mh6A

query
sites
5mh6A

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binding 2-Ketohexanoic acid: R64 (≠ S67), A65 (≠ S68), G66 (= G69), H89 (= H90), R224 (= R217), H272 (= H264), Y280 (≠ I272)
binding magnesium ion: T130 (= T128), A132 (≠ V130), F210 (≠ L203), E211 (≠ A204), M213 (= M206), G225 (= G218), P226 (= P219), V228 (= V221), E230 (≠ T223), D241 (≠ R234), S251 (≠ D243)
binding nicotinamide-adenine-dinucleotide: A65 (≠ S68), G66 (= G69), T86 (≠ D87), H89 (= H90), G142 (= G140), T143 (≠ D141), L144 (≠ I142), R164 (= R162), P196 (= P189), T201 (= T194), V222 (= V215), A223 (≠ G216), R224 (= R217), H272 (= H264), S274 (≠ A266)

5mhaB D-2-hydroxyacid dehydrogenases (d2-hdh) from haloferax mediterranei in complex with a mixture of 2-ketohexanoic acid and 2-hydroxyhexanoic acid, and NADPH (1.57 a resolution)
37% identity, 77% coverage: 63:284/290 of query aligns to 62:294/308 of 5mhaB

query
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5mhaB

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binding 2-Ketohexanoic acid: R66 (≠ S67), R226 (= R217), H274 (= H264), Y282 (≠ I272)
binding (2R)-2-hydroxyhexanoic acid: R66 (≠ S67), A67 (≠ S68), G68 (= G69), H91 (= H90), Y282 (≠ I272)
binding magnesium ion: F212 (≠ L203), E213 (≠ A204), M215 (= M206), D243 (≠ R234)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: A67 (≠ S68), G68 (= G69), T88 (≠ D87), L143 (≠ S139), G144 (= G140), T145 (≠ D141), L146 (≠ I142), R165 (≠ G161), R166 (= R162), S167 (≠ T163), P180 (vs. gap), T197 (≠ L188), P198 (= P189), T203 (= T194), V224 (= V215), A225 (≠ G216), R226 (= R217), H274 (= H264), S276 (≠ A266)

5mhaA D-2-hydroxyacid dehydrogenases (d2-hdh) from haloferax mediterranei in complex with a mixture of 2-ketohexanoic acid and 2-hydroxyhexanoic acid, and NADPH (1.57 a resolution)
37% identity, 77% coverage: 63:284/290 of query aligns to 62:294/308 of 5mhaA

query
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5mhaA

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binding 2-Ketohexanoic acid: R66 (≠ S67), A67 (≠ S68), G68 (= G69), H91 (= H90), R226 (= R217), H274 (= H264), Y282 (≠ I272)
binding magnesium ion: T132 (= T128), A134 (≠ V130)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: A67 (≠ S68), G68 (= G69), T88 (≠ D87), L143 (≠ S139), G144 (= G140), T145 (≠ D141), L146 (≠ I142), R165 (≠ G161), R166 (= R162), S167 (≠ T163), P180 (vs. gap), T197 (≠ L188), P198 (= P189), T203 (= T194), V224 (= V215), A225 (≠ G216), R226 (= R217), H274 (= H264), S276 (≠ A266)

5mh5A D-2-hydroxyacid dehydrogenases (d2-hdh) from haloferax mediterranei in complex with 2-keto-hexanoic acid and NADP+ (1.4 a resolution)
37% identity, 77% coverage: 63:284/290 of query aligns to 62:294/308 of 5mh5A

query
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5mh5A

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binding 2-Ketohexanoic acid: R66 (≠ S67), A67 (≠ S68), G68 (= G69), H91 (= H90), R226 (= R217), H274 (= H264), Y282 (≠ I272)
binding magnesium ion: T132 (= T128), A134 (≠ V130), F212 (≠ L203), E213 (≠ A204), M215 (= M206), D243 (≠ R234)
binding nadp nicotinamide-adenine-dinucleotide phosphate: A67 (≠ S68), G68 (= G69), T88 (≠ D87), G142 (= G138), L143 (≠ S139), G144 (= G140), T145 (≠ D141), L146 (≠ I142), R165 (≠ G161), R166 (= R162), S167 (≠ T163), T197 (≠ L188), P198 (= P189), T203 (= T194), V224 (= V215), A225 (≠ G216), R226 (= R217), H274 (= H264), S276 (≠ A266)

5vg6B Crystal structure of d-isomer specific 2-hydroxyacid dehydrogenase from xanthobacter autotrophicus py2 in complex with NADPH and mes.
36% identity, 74% coverage: 53:268/290 of query aligns to 59:282/315 of 5vg6B

query
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5vg6B

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L

V

A

P

R

F

H

G

G

C

F

E

D

V

V

T

R

M

G

V
x
W

G
x
S

R
|
R

T
|
T

A

P

R
x
K

A

A

-

L

-

E

G

G

V

V

L

-

D

S

Q

C

A

F

A

H

G

G

V

L

A

E

A

A

V

L

P

P

A

G

H

F

-

L

-

A

-

G

-

S

D

E

V

I

V

V

L

V

A

M

L
|
L

P
|
P

L

L

V

T

E

P

S

E

T
|
T

T

R

G

G

M

L

V

M

G

N

A

A

G

E

F

R

L

L

A

A

S

H

M

L

R

P

D

R

G

G

A

A

V

K

L

F

V

I

N

N

V
|
V

G

A

R
|
R

G

G

P

P

L

V

V

V

D

D

T

E

D

A

A

A

L

L

L

I

A

A

E

A

S

L

R

R

T

S

G

G

R

H

L

I

-

A

H

E

A

A

I

T

L

L

D
|
D

V

V

T

F

D

E

P

V

E
|
E

P

P

L

L

P

P

A

V

D

G

H

S

P

P

L

L

W

W

T

A

A

M

P

D

G

N

V

V

V

L

V

V

T

T

P

P

H
|
H

A

L

A
|
A

A
x
S

I

I

active site: M98 (≠ H88), R230 (= R217), D254 (= D240), E259 (= E245), H278 (= H264)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: A74 (≠ S68), R92 (= R82), M102 (≠ V94), L147 (≠ S139), G148 (= G140), D149 (= D141), L150 (≠ I142), W168 (≠ V160), S169 (≠ G161), R170 (= R162), T171 (= T163), K173 (≠ R165), L201 (= L188), P202 (= P189), T207 (= T194), V228 (= V215), R230 (= R217), H278 (= H264), A280 (= A266), S281 (≠ A267)

Sites not aligning to the query:

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 315

3n7uA NAD-dependent formate dehydrogenase from higher-plant arabidopsis thaliana in complex with NAD and azide
29% identity, 68% coverage: 92:288/290 of query aligns to 121:329/351 of 3n7uA

query
sites
3n7uA

V

V

A

S

V
|
V

A

A

E

E

W

D

V

E

V

L

A

M

Q

R

L

I

L

L

A

I

H

L

V

M

R

R

D

N

L

F

A

V

A

P

Y

G

R

Y

T

N

K

Q

Q

V

E

K

C

G

R

E

W

W

E

N

-

V

-

A

-

G

-

I

A

A

H

Y

R

R

T

A

G

Y

T

D

L

L

V

E

G

G

A

K

R

T

V

I

L

G

V

T

F

V

G

G

S

A

G
|
G

D
x
R

I
|
I

G

G

E

K

G

L

V

L

R

L

A

Q

R

R

L

L

V

K

P

P

F

F

G

G

C

C

E

N

V

L

T

L

M

Y

V

H

G
x
D

R
|
R

T

L

A

Q

R

M

A

A

G

P

V

E

L

L

D

E

Q

K

A

E

A

T

G

G

V

A

A

K

A

F

V

V

-

E

-

D

-

L

-

N

-

E

-

M

-

L

P

P

A

K

H

C

D

D

V

V

V

I

V

V

L

I

A

N

L
x
M

P
|
P

L

L

V

T

E

E

S

K

T

T

T

R

G

G

M

M

V

F

G

N

A

K

G

E

F

L

L

I

A

G

S

K

M

L

R

K

D

K

G

G

A

V

V

L

L

I

V

V

N

N

V
x
N

G
x
A

R
|
R

G

G

P

A

L

I

V

M

D

E

T

R

D

Q

A

A

L

V

A

D

E

A

S

V

R

E

T

S

G

G

R

H

L

I

H

G

A

G

I

Y

L

S

-

G

D
|
D

V

V

T

W

D

D

P

P

E
x
Q

P

P

L

A

P

P

A

K

D

D

H

H

P

P

L

W

W

R

T

Y

A

M

P

P

G

N

V

Q

V

A

V

M

T

T

P

P

H
|
H

A

T

A
x
S

A
x
G

I

T

T

T

A

I

D

D

I

A

R

Q

E

L

R

R

I

Y

W

A

A

A

A

G

V

T

A

K

R

D

K

M

V

L

A

E

A

R

H

Y

L

F

active site: R257 (= R217), D281 (= D240), Q286 (≠ E245), H305 (= H264)
binding azide ion: R257 (= R217), H305 (= H264)
binding nicotinamide-adenine-dinucleotide: V123 (= V94), G173 (= G140), R174 (≠ D141), I175 (= I142), D194 (≠ G161), R195 (= R162), M228 (≠ L188), P229 (= P189), N255 (≠ V215), A256 (≠ G216), R257 (= R217), D281 (= D240), H305 (= H264), S307 (≠ A266), G308 (≠ A267)

Sites not aligning to the query:

active site: 119
binding azide ion: 70, 71, 95
binding nicotinamide-adenine-dinucleotide: 95, 119

3jtmA Structure of recombinant formate dehydrogenase from arabidopsis thaliana
29% identity, 68% coverage: 92:288/290 of query aligns to 121:329/351 of 3jtmA

query
sites
3jtmA

V

V

A

S

V

V

A

A

E

E

W

D

V

E

V

L

A

M

Q

R

L

I

L

L

A

I

H
x
L

V

M

R

R

D

N

L

F

A

V

A

P

Y

G

R

Y

T

N

K

Q

Q

V

E

K

C

G

R

E

W

W

E

N

-

V

-

A

-

G

-

I

A

A

H

Y

R

R

T

A

G

Y

T

D

L

L

V

E

G

G

A

K

R

T

V

I

L

G

V

T

F

V

G

G

S

A

G

G

D

R

I

I

G

G

E

K

G

L

V

L

R

L

A

Q

R

R

L

L

V

K

P

P

F

F

G

G

C

C

E

N

V

L

T

L

M

Y

V

H

G

D

R

R

T

L

A

Q

R

M

A

A

G

P

V

E

L

L

D

E

Q

K

A

E

A

T

G

G

V

A

A

K

A

F

V

V

-

E

-

D

-

L

-

N

-

E

-

M

-

L

P

P

A

K

H

C

D

D

V

V

V

I

V

V

L

I

A

N

L

M

P

P

L

L

V

T

E

E

S

K

T

T

T

R

G

G

M

M

V

F

G

N

A

K

G

E

F

L

L

I

A

G

S

K

M

L

R

K

D

K

G

G

A

V

V

L

L

I

V

V

N

N

V

N

G

A

R
|
R

G

G

P

A

L

I

V

M

D

E

T

R

D

Q

A

A

L

V

A

D

E

A

S

V

R

E

T

S

G

G

R

H

L

I

H
x
G

A

G

I

Y

L

S

-

G

D
|
D

V

V

T

W

D

D

P

P

E
x
Q

P

P

L

A

P

P

A

K

D

D

H

H

P

P

L

W

W

R

T

Y

A

M

P

P

G

N

V

Q

V

A

V

M

T

T

P

P

H
|
H

A

T

A

S

A

G

I

T

T

T

A

I

D

D

I

A

R

Q

E

L

R

R

I

Y

W

A

A

A

A

G

V

T

A

K

R

D

K

M

V

L

A

E

A

R

H

Y

L

F

active site: R257 (= R217), D281 (= D240), Q286 (≠ E245), H305 (= H264)
binding azide ion: L134 (≠ H105), G276 (≠ H236)

Sites not aligning to the query:

active site: 119

Q9UBQ7 Glyoxylate reductase/hydroxypyruvate reductase; EC 1.1.1.79; EC 1.1.1.81 from Homo sapiens (Human) (see paper)
29% identity, 90% coverage: 29:288/290 of query aligns to 39:317/328 of Q9UBQ7

query
sites
Q9UBQ7

P

P

L

I

P

P

P

A

E

K

A

E

A

L

R

E

A

R

E

G

V

V

L

A

V

G

A

A

H

H

G

G

I

L

-

L

-

C

-

L

-

S

E

D

P

H

E

V

D

D

A

K

P

R

P

I

L

L

L

D

A

A

L

G

P

A

R

N

L

L

R

K

M

V

V

I

Q

S

L

T

F

M

S

S

S
x
V

G
|
G

V

I

E

D

R

H

W

L

V

A

-

L

-

D

G

E

V

I

V

K

P

K

Q

R

G

G

V

I

R

R

L

V

S

G

N

Y

A

T

D

P

H

D

V

V

H

L

G

T

V

D

A

T

V

T

A

A

E

E

W

L

V

A

V

V

A

S

Q

L

L

L

A

T

H

T

V

C

R

R

D

R

L

L

A

P

A

E

Y

A

R

I

T

E

K

E

Q

V

Q

K

E

N

C

G

R

G

W

W

E

T

A

S

H

W

R

K

T

P

G

L

T

W

L

L

V

C

G

G

-

Y

-

G

-

L

-

T

-

Q

A

S

R

T

V

V

L

G

V

I

F

I

G

G

S

L

G
|
G

D
x
R

I
|
I

G

G

E

Q

G

A

V

I

R

A

A

R

R

R

L

L

V

K

P

P

F

F

G

G

C

V

E

Q

-

R

V

F

T

L

M

Y

V

T

G

G

R
|
R

T
x
Q

A
x
P

R
|
R

-

P

A

E

G

E

V

A

L

A

D

E

Q

F

A

Q

A

A

G

E

V

F

A

V

A

S

V

T

P

P

-

E

-

L

-

A

A

Q

H

S

D

D

V

F

V

I

V

V

L

V

A

A

L

C

P
x
S

L

L

V

T

E

P

S

A

T

T

T

E

G

G

M

L

V

C

G

N

A

K

G

D

F

F

L

F

A

Q

S

K

M

M

R

K

D

E

G

T

A

A

V

V

L

F

V

I

N

N

V
x
I

G

S

R
|
R

G

G

P

D

L

V

V

V

D

N

T

Q

D

D

A

D

L

L

L

Y

A

Q

E

A

S

L

R

A

T

S

G

G

R

K

L

I

H

A

A

A

I

A

-

G

L

L

D
|
D

V

V

T

T

D

S

P

P

E

E

P

P

L

L

P

P

A

T

D

N

H

H

P

P

L

L

W

L

T

T

A

L

P

K

G

N

V

C

V

V

V

I

T

L

P

P

H
|
H

A
x
I

A
x
G

A
x
S

I

A

T

T

A

H

D

R

I

T

R

R

E

N

R

T

I

M

W

S

A

L

V

A

A

A

R

N

K

N

V

L

A

L

A

A

H

G

L

L

VG 83:84 (≠ SG 68:69) binding substrate
GRI 162:164 (≠ GDI 140:142) binding NADP(+)
RQPR 185:188 (≠ RTAR 162:165) binding NADP(+)
S217 (≠ P189) binding NADP(+)
I243 (≠ V215) binding NADP(+)
R245 (= R217) binding substrate
D269 (= D240) binding substrate
HIGS 293:296 (≠ HAAA 264:267) binding substrate
G295 (≠ A266) binding NADP(+)

Q9S7E4 Formate dehydrogenase, chloroplastic/mitochondrial; FDH; NAD-dependent formate dehydrogenase; EC 1.17.1.9 from Arabidopsis thaliana (Mouse-ear cress)
30% identity, 65% coverage: 92:280/290 of query aligns to 154:354/384 of Q9S7E4

query
sites
Q9S7E4

V

V

A

S

V

V

A

A

E

E

W

D

V

E

V

L

A

M

Q

R

L

I

L

L

A

I

H

L

V

M

R

R

D

N

L

F

A

V

A

P

Y

G

R

Y

T

N

K

Q

Q

V

E

K

C

G

R

E

W

W

E

N

-

V

-

A

-

G

-

I

A

A

H

Y

R

R

T

A

G

Y

T

D

L

L

V

E

G

G

A

K

R

T

V

I

L

G

V

T

F

V

G

G

S

A

G

G

D
x
R

I
|
I

G

G

E

K

G

L

V

L

R

L

A

Q

R

R

L

L

V

K

P

P

F

F

G

G

C

C

E

N

V

L

T

L

M

Y

V

H

G
x
D

R

R

T

L

A

Q

R

M

A

A

G

P

V

E

L

L

D

E

Q

K

A

E

A

T

G

G

V

A

A

K

A

F

V

V

-

E

-

D

-

L

-

N

-

E

-

M

-

L

P

P

A

K

H

C

D

D

V

V

V

I

V

V

L

I

A

N

L

M

P
|
P

L
|
L

V
x
T

E
|
E

S
x
K

T

T

T

R

G

G

M

M

V

F

G

N

A

K

G

E

F

L

L

I

A

G

S

K

M

L

R

K

D

K

G

G

A

V

V

L

L

I

V

V

N

N

V
x
N

G

A

R

R

G

G

P

A

L

I

V

M

D

E

T

R

D

Q

A

A

L

V

A

D

E

A

S

V

R

E

T

S

G

G

R

H

L

I

H

G

A

G

I

Y

L

S

-

G

D
|
D

V

V

T

W

D

D

P

P

E

Q

P

P

L

A

P

P

A

K

D

D

H

H

P

P

L

W

W

R

T

Y

A

M

P

P

G

N

V

Q

V

A

V

M

T

T

P

P

H
|
H

A
x
T

A
x
S

A
x
G

I

T

T

T

A

I

D

D

I

A

R

Q

E

L

R

R

I

Y

W

A

A

A

A

G

V

T

RI 207:208 (≠ DI 141:142) binding NAD(+)
D227 (≠ G161) binding NAD(+)
PLTEK 262:266 (≠ PLVES 189:193) binding NAD(+)
N288 (≠ V215) binding NAD(+)
D314 (= D240) binding NAD(+)
HTSG 338:341 (≠ HAAA 264:267) binding NAD(+)

Sites not aligning to the query:

1:29 modified: transit peptide, Chloroplast and mitochondrion

2gcgA Ternary crystal structure of human glyoxylate reductase/hydroxypyruvate reductase (see paper)
29% identity, 90% coverage: 29:288/290 of query aligns to 35:313/324 of 2gcgA

query
sites
2gcgA

P

P

L

I

P

P

P

A

E

K

A

E

A

L

R

E

A

R

E

G

V

V

L

A

V

G

A

A

H

H

G

G

I

L

-

L

-

C

-

L

-
x
L

-

S

E

D

P

H

E

V

D

D

A

K

P

R

P

I

L

L

L

D

A

A

L

G

P

A

R

N

L

L

R

K

M

V

V

I

Q

S

L

T

F

M

S
|
S

S
x
V

G
|
G

V

I

E

D

R

H

W

L

V

A

-

L

-

D

G

E

V

I

V

K

P

K

Q

R

G

G

V

I

R

R

L

V

S

G

N

Y

A

T

D

P

H

D

V

V

H
x
L

G

T

V

D

A

T

V
x
T

A

A

E

E

W

L

V

A

V

V

A

S

Q

L

L

L

A

T

H

T

V

C

R

R

D

R

L

L

A

P

A

E

Y

A

R

I

T

E

K

E

Q

V

Q

K

E

N

C

G

R

G

W

W

E

T

A

S

H

W

R

K

T

P

G

L

T

W

L

L

V

C

G

G

-

Y

-

G

-

L

-

T

-

Q

A

S

R

T

V

V

L

G

V

I

F

I

G
|
G

S

L

G
|
G

D

R

I
|
I

G

G

E

Q

G

A

V

I

R

A

A

R

R

R

L

L

V

K

P

P

F

F

G

G

C

V

E

Q

-

R

V

F

T

L

M

Y

V

T

G
|
G

R
|
R

T

Q

A

P

R
|
R

-

P

A

E

G

E

V

A

L

A

D

E

Q

F

A

Q

A

A

G

E

V

F

A

V

A

S

V

T

P

P

-

E

-

L

-

A

A

Q

H

S

D

D

V

F

V

I

V

V

L

V

A

A

L
x
C

P
x
S

L

L

V

T

E

P

S

A

T
|
T

T

E

G

G

M

L

V

C

G

N

A

K

G

D

F

F

L

F

A

Q

S

K

M

M

R

K

D

E

G

T

A

A

V

V

L

F

V

I

N

N

V
x
I

G

S

R
|
R

G

G

P

D

L

V

V

V

D

N

T

Q

D

D

A

D

L

L

L

Y

A

Q

E

A

S

L

R

A

T

S

G

G

R

K

L

I

H

A

A

A

I

A

-

G

L

L

D
|
D

V

V

T

T

D

S

P

P

E
|
E

P

P

L

L

P

P

A

T

D

N

H

H

P

P

L

L

W

L

T

T

A

L

P

K

G

N

V

C

V

V

V

I

T

L

P

P

H
|
H

A

I

A
x
G

A

S

I

A

T

T

A

H

D

R

I

T

R

R

E

N

R

T

I

M

W

S

A

L

V

A

A

A

R

N

K

N

V

L

A

L

A

A

H

G

L

L

active site: L103 (≠ H90), R241 (= R217), D265 (= D240), E270 (= E245), H289 (= H264)
binding (2r)-2,3-dihydroxypropanoic acid: L55 (vs. gap), S78 (= S67), V79 (≠ S68), G80 (= G69), R241 (= R217), H289 (= H264)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: V79 (≠ S68), T107 (≠ V94), G156 (= G138), G158 (= G140), I160 (= I142), G180 (= G161), R181 (= R162), R184 (= R165), C212 (≠ L188), S213 (≠ P189), T218 (= T194), I239 (≠ V215), R241 (= R217), D265 (= D240), H289 (= H264), G291 (≠ A266)

4zqbB Crystal structure of NADP-dependent dehydrogenase from rhodobactersphaeroides in complex with NADP and sulfate
33% identity, 88% coverage: 16:269/290 of query aligns to 29:284/316 of 4zqbB

query
sites
4zqbB

L

L

P

P

G

G

V

E

R

R

A

I

V

D

V

G

Y

F

R

R

P

E

G

L

A

S

P

P

L

-

P

-

E

-

A

A

A

E

R

R

A

A

E

E

V

V

L

D

V

I

A

A

H

I

G

V

I

A

E

N

P

P

E

D

D

-

A

-

P

P

P

A

L

D

L

L

A

A

A

E

L

L

P

P

R

N

L

L

R

V

M

W

V

I

Q

H

L

S

F

L

S

W

S

A

G

G

V

V

E

E

R

R

W

L

V

V

G

A

V

E

V

L

P

G

Q

H

G

L

V

A

R

R

-

P

-

I

L

V

S
x
R

N

L

A

V

D

D

H

P

V

E

H
x
L

G

A

V

R

A

T

V
x
M

A

A

E

E

W

A

V

A

V

L

A

A

Q

W

L

T

L

Y

A

Y

H

L

V

F

R

R

D

D

L

M

A

P

A

A

Y

Y

R

A

T

A

K

Q

Q

Q

Q

R

E

A

C

R

R

V

W

W

E

K

A

G

H

L

R

P

T

Y

G

K

T

R

L

P

V

E

G

R

A

T

R

T

V

V

L

G

V

V

F

L

G
|
G

S
x
L

G
|
G

D
x
E

I
x
L

G

G

E

A

G

A

V

A

R

A

A

L

R

R

L

L

V

R

P

D

F

A

G

G

C

F

E

D

V

V

-

H

-

G

-
x
W

-
x
S

-
x
R

-
x
S

-

P

-
x
K

T

E

M

I

V

A

G

G

R

V

T

T

A

C

R

H

A

A

G

G

-

E

V

T

L

L

D

E

Q

R

A

M

A

L

G

G

V

Q

A

V

A

-

V

-

P

-

A

-

H

-

D

E

V

I

V

L

V

V

L

C

A

L

L
|
L

P
|
P

L

L

V

T

E

G

S

E

T

T

T

R

G

G

M

L

V

L

G

D

A

A

G

R

F

R

L

L

A

A

S

C

M

L

R

P

D

E

G

G

A

A

V

Q

L

I

V

V

N

N

V
x
F

G

A

R
|
R

G

G

P

P

L

I

V

L

D

D

T

S

D

A

A

A

L

L

L

I

A

E

E

A

S

L

R

D

T

S

G

G

R

R

L

I

-

G

H

H

A

A

I

V

L

L

D

D

V

V

T

F

D

E

P

V

E
|
E

P

P

L

L

P

P

A

E

D

A

H

S

P

P

L

F

W

W

T

G

A

H

P

P

G

K

V

V

V

T

V

V

T

L

P

P

H
|
H

A

I

A
x
S

A
|
A

I

A

T

T

active site: L99 (≠ H90), R231 (= R217), E260 (= E245), H279 (= H264)
binding nadp nicotinamide-adenine-dinucleotide phosphate: R93 (≠ S84), M103 (≠ V94), G147 (= G138), L148 (≠ S139), G149 (= G140), E150 (≠ D141), L151 (≠ I142), W169 (vs. gap), S170 (vs. gap), R171 (vs. gap), S172 (vs. gap), K174 (vs. gap), L202 (= L188), P203 (= P189), F229 (≠ V215), R231 (= R217), H279 (= H264), S281 (≠ A266), A282 (= A267)

Sites not aligning to the query:

binding nadp nicotinamide-adenine-dinucleotide phosphate: 316

7jqiA Structure of wild type glyoxylate/hydroxypyruvate reductase a from escherichia coli in complex with alpha-ketoglutarate and NADP+
34% identity, 62% coverage: 91:269/290 of query aligns to 96:280/312 of 7jqiA

query
sites
7jqiA

G

G

V

E

A

Q

V
x
M

A

Q

E

E

W

Y

V

A

V

V

A

S

Q

Q

L

V

L

L

A

H

H

W

V

F

R

R

D

R

L

F

A

D

Y

Y

R

R

T

I

K

Q

Q

Q

Q

N

E

S

C

S

R

H

W

W

-

Q

-

P

-

L

-

P

E

E

A

Y

H

H

R

R

T

E

G

D

T

F

L

T

V

I

G

G

A

-

R

-

V

-

L

-

V

I

F

L

G

G

S
x
A

G
|
G

D
x
V

I
x
L

G

G

E

S

G

K

V

V

R

A

A

Q

R

S

L

L

V

Q

P

T

F

W

G

R

C

F

E

P

V

L

T

R

M

C

V
x
W

G
x
S

R
|
R

T
|
T

A

R

R
x
K

A

S

-

W

-

P

G

G

V

V

L

Q

D

S

Q

F

A

A

A

G

G

R

-

E

-

L

V

S

A

A

A

F

V

L

P

S

A

Q

H

C

D

R

V

V

V

L

V

I

L

N

A

L

L
|
L

P
|
P

L

N

V

T

E

P

S

E

T

T

T

V

G

G

M

I

V

I

G

N

A

Q

G

Q

F

L

L

L

A

E

S

K

M

L

R

P

D

D

G

G

A

A

V

Y

L

L

V

L

N

N

V
x
L

G

A

R
|
R

G

G

P

V

L

H

V

V

D

V

T

E

D

D

A

D

L

L

A

A

E

A

S

L

R

D

T

S

G

G

R

K

L

V

H

K

-

G

A

A

I

M

L

L

D

D

V

V

T

F

D

N

P

R

E

E

P

P

L

L

P

P

A

P

D

E

H

S

P

P

L

L

W

W

T

Q

A

H

P

P

G

R

V

V

V

T

V

I

T

T

P

P

H
|
H

A

V

A

A

A
|
A

I

I

T

T

binding 2-oxoglutaric acid: R227 (= R217), H275 (= H264)
binding nadp nicotinamide-adenine-dinucleotide phosphate: M99 (≠ V94), A144 (≠ S139), G145 (= G140), V146 (≠ D141), L147 (≠ I142), W165 (≠ V160), S166 (≠ G161), R167 (= R162), T168 (= T163), K170 (≠ R165), L198 (= L188), P199 (= P189), L225 (≠ V215), R227 (= R217), H275 (= H264), A278 (= A267)

Sites not aligning to the query:

6p35A 2.5 angstrom structure of wild type glyoxylate/hydroxypyruvate reductase a from escherichia coli in complex with 2-keto arginine and NADP
34% identity, 62% coverage: 91:269/290 of query aligns to 96:280/312 of 6p35A

query
sites
6p35A

G

G

V

E

A

Q

V
x
M

A

Q

E

E

W

Y

V

A

V

V

A

S

Q

Q

L

V

L

L

A

H

H

W

V

F

R

R

D

R

L

F

A

D

Y

Y

R

R

T

I

K

Q

Q

Q

Q

N

E

S

C

S

R

H

W

W

-

Q

-

P

-

L

-

P

E

E

A

Y

H

H

R

R

T

E

G

D

T

F

L

T

V

I

G

G

A

-

R

-

V

-

L

-

V

I

F

L

G

G

S
x
A

G
|
G

D
x
V

I
x
L

G

G

E

S

G

K

V

V

R

A

A

Q

R

S

L

L

V

Q

P

T

F

W

G

R

C

F

E

P

V

L

T

R

M

C

V
x
W

G
x
S

R
|
R

T
|
T

A

R

R
x
K

A

S

-

W

-

P

G

G

V

V

L

Q

D

S

Q

F

A

A

A

G

G

R

-

E

-

L

V

S

A

A

A

F

V

L

P

S

A

Q

H

C

D

R

V

V

V

L

V

I

L

N

A

L

L
|
L

P
|
P

L

N

V

T

E

P

S

E

T

T

T

V

G

G

M

I

V

I

G

N

A

Q

G

Q

F

L

L

L

A

E

S

K

M

L

R

P

D

D

G

G

A

A

V

Y

L

L

V

L

N

N

V
x
L

G

A

R
|
R

G

G

P

V

L

H

V

V

D

V

T

E

D

D

A

D

L

L

A

A

E

A

S

L

R

D

T

S

G

G

R

K

L

V

H

K

-

G

A

A

I

M

L

L

D

D

V

V

T

F

D

N

P

R

E

E

P

P

L

L

P

P

A

P

D

E

H

S

P

P

L

L

W

W

T

Q

A

H

P

P

G

R

V

V

V

T

V

I

T

T

P

P

H
|
H

A

V

A
|
A

I

I

T
|
T

binding nadp nicotinamide-adenine-dinucleotide phosphate: M99 (≠ V94), A144 (≠ S139), G145 (= G140), V146 (≠ D141), L147 (≠ I142), W165 (≠ V160), S166 (≠ G161), R167 (= R162), T168 (= T163), K170 (≠ R165), L198 (= L188), P199 (= P189), L225 (≠ V215), R227 (= R217), H275 (= H264), A277 (= A266), A278 (= A267)
binding 5-[(diaminomethylidene)amino]-2-oxopentanoic acid: R227 (= R217), H275 (= H264), T280 (= T269)

Sites not aligning to the query:

7jqhA Structure of wild type glyoxylate/hydroxypyruvate reductase a from escherichia coli in complex with phosphate and NADP+
34% identity, 62% coverage: 91:269/290 of query aligns to 97:281/313 of 7jqhA

query
sites
7jqhA

G

G

V

E

A

Q

V
x
M

A

Q

E

E

W

Y

V

A

V

V

A

S

Q

Q

L

V

L

L

A

H

H

W

V

F

R

R

D

R

L

F

A

D

Y

Y

R

R

T

I

K

Q

Q

Q

Q

N

E

S

C

S

R

H

W

W

-

Q

-

P

-

L

-

P

E

E

A

Y

H

H

R

R

T

E

G

D

T

F

L

T

V

I

G

G

A

-

R

-

V

-

L

-

V

I

F

L

G

G

S
x
A

G
|
G

D
x
V

I
x
L

G

G

E

S

G

K

V

V

R

A

A

Q

R

S

L

L

V

Q

P

T

F

W

G

R

C

F

E

P

V

L

T

R

M

C

V
x
W

G
x
S

R
|
R

T
|
T

A

R

R
x
K

A

S

-

W

-

P

G

G

V

V

L

Q

D

S

Q

F

A

A

A

G

G

R

-

E

-

L

V

S

A

A

A

F

V

L

P

S

A

Q

H

C

D

R

V

V

V

L

V

I

L

N

A

L

L
|
L

P
|
P

L

N

V

T

E

P

S

E

T

T

T

V

G

G

M

I

V

I

G

N

A

Q

G

Q

F

L

L

L

A

E

S

K

M

L

R

P

D

D

G

G

A

A

V

Y

L

L

V

L

N

N

V
x
L

G
x
A

R
|
R

G

G

P

V

L

H

V

V

D

V

T

E

D

D

A

D

L

L

A

A

E

A

S

L

R

D

T

S

G

G

R

K

L

V

H

K

-

G

A

A

I

M

L

L

D
|
D

V

V

T

F

D

N

P

R

E

E

P

P

L

L

P

P

A

P

D

E

H

S

P

P

L

L

W

W

T

Q

A

H

P

P

G

R

V

V

V

T

V

I

T

T

P

P

H
|
H

A

V

A

A

A
|
A

I

I

T

T

binding nadp nicotinamide-adenine-dinucleotide phosphate: M100 (≠ V94), A145 (≠ S139), G146 (= G140), V147 (≠ D141), L148 (≠ I142), W166 (≠ V160), S167 (≠ G161), R168 (= R162), T169 (= T163), K171 (≠ R165), L199 (= L188), P200 (= P189), L226 (≠ V215), A227 (≠ G216), R228 (= R217), D252 (= D240), H276 (= H264), A279 (= A267)

Sites not aligning to the query:

binding nadp nicotinamide-adenine-dinucleotide phosphate: 66, 90, 313

3bazA Structure of hydroxyphenylpyruvate reductase from coleus blumei in complex with NADP+ (see paper)
29% identity, 82% coverage: 53:289/290 of query aligns to 59:302/311 of 3bazA

query
sites
3bazA

L

L

L

I

A

D

A

A

L

L

P

P

R

K

L

L

R

E

M

I

V

V

Q

S

L

S

F

F

S

S

S
x
V

G

G

V

L

E

D

R

K

-

V

-

D

W

L

V

I

G

K

V

C

V

E

P

E

Q

K

G

G

V

V

R

R

L

V

S

T

N

N

A

T

D

P

H

D

V

V

H
x
L

G

T

V

D

A

D

V

V

A

A

E

D

W

L

V

A

V

I

A

G

Q

L

L

I

L

L

A

A

H

V

V

L

R

R

D

R

L

I

A

C

A

E

Y

C

R

D

T

K

K

Y

Q

V

Q

R

E

R

C

G

R

A

W

W

-

K

-

F

-

G

E

D

A

F

H

K

R

L

T

T

G

T

T

K

L

F

V

S

G

G

A

K

R

R

V

V

L

G

V

I

F

I

G
|
G

S
x
L

G
|
G

D
x
R

I
|
I

G

G

E

L

G

A

V

V

R

A

A

E

R

R

L

A

V

E

P

A

F

F

G

D

C

C

E

P

V

I

T

S

M

Y

V

F

G
x
S

R
|
R

T
x
S

A

K

R

K

A

P

G

N

V

T

L

N

D

Y

Q

T

A

Y

G

G

-

S

-

V

V

V

A

E

A

L

V

A

P

S

A

N

H

S

D

D

V

I

V

L

V

V

L

V

A

A

L
x
C

P
|
P

L

L

V

T

E

P

S

E

T
|
T

T

T

G

H

M

I

V

I

G

N

A

R

G

E

F

V

L

I

A

D

S

A

M

L

R

G

D

P

G

K

A

G

V

V

L

L

V

I

N

N

V
x
I

G
|
G

R
|
R

G

G

P

P

L

H

V

V

D

D

T

E

D

P

A

E

L

L

L

V

A

S

E

A

S

L

R

V

T

E

G

G

R

R

L

L

-

G

H

G

A
|
A

I

G

L

L

D
|
D

V

V

T

F

D

E

P

R

E
|
E

P

P

L

-

P

E

A

V

D

P

H

E

P

K

L

L

W

F

T

G

A

L

P

E

G

N

V

V

V

L

T

L

P

P

H
|
H

A

V

A
x
G

A

S

I

G

T

T

A

V

D

E

I

T

R

R

E

K

R

V

I

M

W

A

A

D

A

L

V

V

A

V

R

G

K

N

V

L

A

E

A

A

H

H

L

F

T

S

active site: L98 (≠ H90), R230 (= R217), A251 (= A237), D254 (= D240), E259 (= E245), H277 (= H264)
binding nadp nicotinamide-adenine-dinucleotide phosphate: V74 (≠ S68), G149 (= G138), L150 (≠ S139), G151 (= G140), R152 (≠ D141), I153 (= I142), S172 (≠ G161), R173 (= R162), S174 (≠ T163), C201 (≠ L188), P202 (= P189), T207 (= T194), I228 (≠ V215), G229 (= G216), R230 (= R217), D254 (= D240), H277 (= H264), G279 (≠ A266)

Q65CJ7 Hydroxyphenylpyruvate reductase; HPPR; EC 1.1.1.237 from Plectranthus scutellarioides (Coleus) (Solenostemon scutellarioides) (see paper)
29% identity, 82% coverage: 53:289/290 of query aligns to 61:304/313 of Q65CJ7

query
sites
Q65CJ7

L

L

L

I

A

D

A

A

L

L

P

P

R

K

L

L

R

E

M

I

V

V

Q

S

L

S

F

F

S

S

S

V

G

G

V

L

E

D

R

K

-

V

-

D

W

L

V

I

G

K

V

C

V

E

P

E

Q

K

G

G

V

V

R

R

L

V

S

T

N

N

A

T

D

P

H

D

V

V

H

L

G

T

V

D

A

D

V

V

A

A

E

D

W

L

V

A

V

I

A

G

Q

L

L

I

L

L

A

A

H

V

V

L

R

R

D

R

L

I

A

C

A

E

Y

C

R

D

T

K

K

Y

Q

V

Q

R

E

R

C

G

R

A

W

W

-

K

-

F

-

G

E

D

A

F

H

K

R

L

T

T

G

T

T

K

L

F

V

S

G

G

A

K

R

R

V

V

L

G

V

I

F

I

G

G

S
x
L

G
|
G

D
x
R

I
|
I

G

G

E

L

G

A

V

V

R

A

A

E

R

R

L

A

V

E

P

A

F

F

G

D

C

C

E

P

V

I

T

S

M

Y

V

F

G
x
S

R
|
R

T
x
S

A

K

R

K

A

P

G

N

V

T

L

N

D

Y

Q

T

A

Y

G

G

-

S

-

V

V

V

A

E

A

L

V

A

P

S

A

N

H

S

D

D

V

I

V

L

V

V

L

V

A

A

L

C

P

P

L

L

V

T

E

P

S

E

T

T

G

H

M

I

V

I

G

N

A

R

G

E

F

V

L

I

A

D

S

A

M

L

R

G

D

P

G

K

A

G

V

V

L

L

V

I

N

N

V
x
I

G

G

R

R

G

G

P

P

L

H

V

V

D

D

T

E

D

P

A

E

L

L

L

V

A

S

E

A

S

L

R

V

T

E

G

G

R

R

L

L

-

G

H

G

A

A

I

G

L

L

D
|
D

V

V

T

F

D

E

P

R

E

E

P

P

L

-

P

E

A

V

D

P

H

E

P

K

L

L

W

F

T

G

A

L

P

E

G

N

V

V

V

L

T

L

P

P

H

H

A

V

A

G

A

S

I

G

T

T

A

V

D

E

I

T

R

R

E

K

R

V

I

M

W

A

A

D

A

L

V

V

A

V

R

G

K

N

V

L

A

E

A

A

H

H

L

F

T

S

LGRI 152:155 (≠ SGDI 139:142) binding NADP(+)
SRS 174:176 (≠ GRT 161:163) binding NADP(+)
I230 (≠ V215) binding NADP(+)
D256 (= D240) binding NADP(+)

1wwkA Crystal structure of phosphoglycerate dehydrogenase from pyrococcus horikoshii ot3
46% identity, 36% coverage: 182:284/290 of query aligns to 195:298/304 of 1wwkA

query
sites
1wwkA

D

D

V

V

V

T

L

I

A

H

L
x
V

P
|
P

L

L

V

V

E

E

S

S

T
|
T

T

Y

G

H

M

L

V

I

G

N

A

E

G

E

F

R

L

L

A

K

S

L

M

M

R

K

D

K

G

T

A

A

V

I

L

L

V

I

N

N

V
x
T

G
x
S

R
|
R

G

G

P

P

L

V

V

V

D

D

T

T

D

N

A

A

L

L

L

V

A

K

E

A

S

L

R

K

T

E

G

G

R

W

L

I

H

A

-

G

A

A

I

G

L

L

D
|
D

V

V

T

F

D

E

P

E

E
|
E

P

P

L

L

P

P

A

K

D

D

H

H

P

P

L

L

W

T

T

K

A

F

P

D

G

N

V

V

V

L

T

T

P

P

H
|
H

A

I

A
x
G

A

A

I

S

T

T

A

V

D

E

I

A

R

Q

E

E

R

R

I

A

W

G

A

V

A

E

V

V

A

A

R

E

K

K

V

V

active site: R230 (= R217), D254 (= D240), E259 (= E245), H278 (= H264)
binding nicotinamide-adenine-dinucleotide: V201 (≠ L188), P202 (= P189), T207 (= T194), T228 (≠ V215), S229 (≠ G216), D254 (= D240), H278 (= H264), G280 (≠ A266)

Sites not aligning to the query:

active site: 96
binding nicotinamide-adenine-dinucleotide: 100, 146, 147, 148, 149, 150, 168, 169, 170

5aovA Ternary crystal structure of pyrococcus furiosus glyoxylate hydroxypyruvate reductase in presence of glyoxylate (see paper)
29% identity, 79% coverage: 58:287/290 of query aligns to 66:311/334 of 5aovA

query
sites
5aovA

P

P

R

R

L

L

R

R

M

I

V

V

Q

A

L

N

F
x
Y

S
x
A

S
x
V

G
|
G

V

Y

E

D

R

N

W

-

V

I

G

D

V

V

-

E

-

A

V

T

P

R

Q

R

G

G

V

I

R

Y

L

V

S

T

N

N

A

T

D

P

H

D

V

V

H
x
L

G

T

V

N

A

A

V
x
T

A

A

E

D

W

H

V

A

V

F

A

A

Q

L

L

L

A

A

H

T

V

A

R

R

D

H

L

V

A

V

A

K

Y

G

R

D

T

K

K

F

Q

V

Q

R

E

S

C

G

R

E

W

W

E

K

A

-

H

-

R

R

T

K

G

G

-

I

-

A

-

W

-

H

-

P

-

K

-

W

-

F

-

L

-

G

-

Y

T

E

L

L

V

Y

G

G

A

K

R

T

V

I

L

G

V

I

F

V

G

G

S
x
F

G
|
G

D
x
R

I
|
I

G

G

E

Q

G

A

V

I

R

A

A

R

R

R

L

A

V

K

P

G

F

F

G

N

C

M

E

R

V

I

T

L

M

Y

V

Y

G
x
S

R
|
R

T

T

A

-

R

R

A

K

G

S

V

Q

L

A

D

E

Q

K

A

E

A

L

G

G

V

A

A

E

A

Y

V

R

P

P

A

L

H

E

-

E

-

V

-

L

-

K

-

E

-

S

D

D

V

F

V

V

V

I

L

L

A
|
A

L
x
V

P
|
P

L

L

V

T

E

K

S

E

T
|
T

T

M

G

Y

M

M

V

I

G

N

A

E

G

E

F

R

L

L

A

K

S

L

M

M

R

K

D

P

G

T

A

A

V

I

L

L

V

V

N

N

V
x
I

G
x
A

R
|
R

G

G

P

K

L

V

V

V

D

D

T

T

D

K

A

A

L

L

L

I

A

K

E

A

S

L

R

K

T

E

G

G

R

W

L

I