SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_084704279.1 NCBI__GCF_000744815.1:WP_084704279.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

5hudE Non-covalent complex of and dahp synthase and chorismate mutase from corynebacterium glutamicum with bound transition state analog (see paper)
38% identity, 74% coverage: 28:106/107 of query aligns to 4:82/84 of 5hudE

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binding 8-hydroxy-2-oxa-bicyclo[3.3.1]non-6-ene-3,5-dicarboxylic acid: R29 (= R53), S33 (= S57), I36 (≠ V60), R40 (= R64), L48 (= L72), V49 (≠ S73), R52 (= R76), E53 (= E77), L76 (= L100)

P9WIC1 Intracellular chorismate mutase; CM; EC 5.4.99.5 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
44% identity, 62% coverage: 32:97/107 of query aligns to 29:93/105 of P9WIC1

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P9WIC1

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R61 (= R64) Important for catalysis; mutation to K: It is catalytically catastrophic, but strongly activated by AroG.

Sites not aligning to the query:

101 Important for activation via AroG; G→A: The catalytic efficiency and the affinity are 5 and 3-fold lower than the wild-type. The activation by AroG is 10-fold lower than the wild-type.
102 Important for activation via AroG; R→A: The catalytic efficiency and the affinity are slightly modified. The activation by AroG is 2-fold lower than the wild-type.
103 Important for activation via AroG; L→A: The catalytic efficiency and the affinity are identical to the wild-type. The activation by AroG is 10-fold lower than the wild type.

5ckxD Non-covalent complex of dahp synthase and chorismate mutase from mycobacterium tuberculosis with bound transition state analog and feedback effectors tyrosine and phenylalanine (see paper)
44% identity, 62% coverage: 32:97/107 of query aligns to 3:67/79 of 5ckxD

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5ckxD

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binding 8-hydroxy-2-oxa-bicyclo[3.3.1]non-6-ene-3,5-dicarboxylic acid: R24 (= R53), S28 (= S57), I31 (≠ V60), R35 (= R64), R47 (= R76), E48 (= E77)

Sites not aligning to the query:

binding 8-hydroxy-2-oxa-bicyclo[3.3.1]non-6-ene-3,5-dicarboxylic acid: 70

Query Sequence

>WP_084704279.1 NCBI__GCF_000744815.1:WP_084704279.1
MSTELVPTQPNARPETGVRAEAPDAAAAEALIAQARERIDALDGELLRIVKERMAVSARV
QRARIATGGPRLSLAREQQILARFHAELGRAGTELGMLLLELSRGRA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory