SitesBLAST
Comparing WP_084931115.1 NCBI__GCF_002095475.1:WP_084931115.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
4hpnA Crystal structure of a proposed galactarolactone cycloisomerase from agrobacterium tumefaciens, target efi-500704, with bound ca, ordered loops (see paper)
44% identity, 99% coverage: 1:386/388 of query aligns to 1:373/378 of 4hpnA
- active site: F19 (= F20), G50 (= G53), R53 (≠ Q56), T134 (= T138), K164 (= K172), K166 (= K174), D194 (= D203), N196 (= N205), E220 (= E229), G245 (= G254), E246 (= E255), T247 (≠ C256), Q267 (= Q276), D269 (= D278), H296 (= H305), V297 (= V306), W298 (= W307), R320 (≠ T329), E329 (= E342), F330 (= F343), H334 (= H347)
- binding calcium ion: D194 (= D203), D209 (≠ R218), E220 (= E229), G237 (≠ E246), E246 (= E255)
5olcC Crystal structure of the 3,6-anhydro-d-galactonate cycloisomerase from zobellia galactanivorans (see paper)
38% identity, 99% coverage: 2:386/388 of query aligns to 2:348/351 of 5olcC
- active site: K148 (= K172), K150 (= K174), D178 (= D203), N180 (= N205), E204 (= E229), G229 (= G254), E230 (= E255), D253 (= D278), H280 (= H305), E304 (= E342), E309 (≠ H347)
- binding magnesium ion: D178 (= D203), E204 (= E229), E230 (= E255)
3ozmD Crystal structure of enolase superfamily member from bordetella bronchiseptica complexed with mg, m-xylarate and l-lyxarate
34% identity, 100% coverage: 1:388/388 of query aligns to 2:374/381 of 3ozmD
- active site: M20 (≠ T19), G53 (= G50), D56 (≠ G53), S143 (≠ T138), K170 (= K172), K172 (= K174), D200 (= D203), N202 (= N205), E226 (= E229), G252 (= G254), E253 (= E255), N254 (≠ C256), Q274 (= Q276), D276 (= D278), H303 (= H305), T304 (≠ V306), F305 (≠ W307), E328 (= E342), I331 (≠ R345), H333 (= H347)
- binding L-arabinaric acid: K172 (= K174), D200 (= D203), N202 (= N205), E253 (= E255), H303 (= H305), F305 (≠ W307), E328 (= E342)
- binding magnesium ion: D200 (= D203), E226 (= E229), E253 (= E255)
3ozmA Crystal structure of enolase superfamily member from bordetella bronchiseptica complexed with mg, m-xylarate and l-lyxarate
34% identity, 100% coverage: 1:388/388 of query aligns to 2:374/386 of 3ozmA
- active site: M20 (≠ T19), G53 (= G50), D56 (≠ G53), S143 (≠ T138), K170 (= K172), K172 (= K174), D200 (= D203), N202 (= N205), E226 (= E229), G252 (= G254), E253 (= E255), N254 (≠ C256), Q274 (= Q276), D276 (= D278), H303 (= H305), T304 (≠ V306), F305 (≠ W307), E328 (= E342), I331 (≠ R345), H333 (= H347)
- binding D-xylaric acid: S24 (= S23), K29 (≠ P28), Y146 (= Y141), K170 (= K172), K172 (= K174), D200 (= D203), N202 (= N205), E253 (= E255), H303 (= H305), F305 (≠ W307), E328 (= E342)
- binding magnesium ion: D200 (= D203), E226 (= E229), E253 (= E255)
3sjnA Crystal structure of enolase spea_3858 (target efi-500646) from shewanella pealeana with magnesium bound
32% identity, 99% coverage: 1:386/388 of query aligns to 2:370/373 of 3sjnA
- active site: S46 (vs. gap), L49 (vs. gap), T139 (= T138), K165 (= K172), G167 (≠ K174), M171 (≠ L178), D198 (= D203), A200 (= A204), E225 (= E229), I247 (≠ V251), G250 (= G254), E251 (= E255), S252 (≠ C256), Q272 (= Q276), D274 (= D278), H301 (= H305), G302 (≠ V306), F303 (≠ W307), M325 (≠ I341), E326 (= E342), Q329 (≠ R345), S331 (≠ H347)
- binding magnesium ion: D198 (= D203), E225 (= E229), E251 (= E255)
3op2A Crystal structure of putative mandelate racemase from bordetella bronchiseptica rb50 complexed with 2-oxoglutarate/phosphate
33% identity, 100% coverage: 1:388/388 of query aligns to 2:369/375 of 3op2A
- active site: M20 (≠ T19), G53 (= G50), D56 (≠ G53), S138 (≠ T138), K165 (= K172), K167 (= K174), D195 (= D203), N197 (= N205), E221 (= E229), G247 (= G254), E248 (= E255), N249 (≠ C256), Q269 (= Q276), D271 (= D278), H298 (= H305), T299 (≠ V306), F300 (≠ W307), E323 (= E342), I326 (≠ R345), H328 (= H347)
- binding 2-oxoglutaric acid: K165 (= K172), K167 (= K174), D195 (= D203), E248 (= E255), H298 (= H305), E323 (= E342)
- binding magnesium ion: D195 (= D203), E221 (= E229), E248 (= E255)
3bjsB Crystal structure of a member of enolase superfamily from polaromonas sp. Js666
32% identity, 92% coverage: 29:386/388 of query aligns to 27:366/376 of 3bjsB
- active site: P49 (≠ Q51), S52 (≠ P54), L93 (≠ Q94), G136 (≠ T138), K164 (= K172), R166 (≠ K174), D194 (= D203), N196 (= N205), E220 (= E229), G246 (= G254), E247 (= E255), N248 (≠ C256), Q268 (= Q276), D270 (= D278), H297 (= H305), S298 (≠ V306), S299 (≠ W307), E322 (≠ A330), C324 (≠ A332), K327 (= K346)
- binding magnesium ion: D194 (= D203), E220 (= E229), E247 (= E255)
Sites not aligning to the query:
2o56A Crystal structure of a member of the enolase superfamily from salmonella typhimurium
29% identity, 91% coverage: 34:385/388 of query aligns to 22:385/392 of 2o56A
- active site: K165 (= K172), D167 (≠ K174), M171 (≠ L178), L186 (= L180), E214 (≠ D203), H216 (≠ N205), E240 (= E229), G265 (= G254), E266 (= E255), Q287 (= Q276), D289 (= D278), H316 (= H305), E341 (≠ F343), A346 (≠ N348)
- binding magnesium ion: E214 (≠ D203), E240 (= E229), E266 (= E255)
4e6mA Crystal structure of putative dehydratase protein from salmonella enterica subsp. Enterica serovar typhimurium (salmonella typhimurium)
28% identity, 99% coverage: 1:386/388 of query aligns to 2:395/401 of 4e6mA
- active site: L45 (vs. gap), G48 (= G53), K169 (= K172), D171 (≠ K174), I175 (vs. gap), E223 (≠ D203), H225 (≠ N205), E249 (= E229), G274 (= G254), E275 (= E255), R276 (≠ C256), Q296 (= Q276), D298 (= D278), H325 (= H305), C327 (≠ W307), E350 (≠ A330), A355 (≠ L335)
- binding magnesium ion: E223 (≠ D203), E249 (= E229), E275 (= E255)
4h83F Crystal structure of mandelate racemase/muconate lactonizing enzyme (efi target:502127)
29% identity, 99% coverage: 1:386/388 of query aligns to 2:365/368 of 4h83F
- active site: F20 (= F20), D51 (≠ G50), I139 (≠ T138), K166 (= K172), K168 (= K174), I196 (≠ V202), D197 (= D203), A198 (= A204), N199 (= N205), E223 (= E229), G249 (= G254), Q250 (≠ E255), N271 (≠ Q276), D273 (= D278), H300 (= H305), E301 (≠ V306), E302 (≠ A312), E320 (≠ A330), D325 (= D344)
- binding bicarbonate ion: R220 (≠ L226), I318 (≠ F328)
3no1A Crystal structure of mandelate racemase/muconate lactonizing enzyme from a marine actinobacterium in complex with magnesium
30% identity, 93% coverage: 25:386/388 of query aligns to 13:353/355 of 3no1A
- active site: D39 (≠ G50), I127 (≠ T138), K154 (= K172), K156 (= K174), I184 (≠ V202), D185 (= D203), A186 (= A204), N187 (= N205), E211 (= E229), G237 (= G254), Q238 (≠ E255), N259 (≠ Q276), D261 (= D278), H288 (= H305), E289 (≠ V306), E290 (≠ A312), E308 (≠ A330), D313 (= D344)
- binding magnesium ion: D185 (= D203), E211 (= E229), Q238 (≠ E255)
3ck5A Crystal structure of a racemase from streptomyces coelicolor a3(2) with bound magnesium
29% identity, 97% coverage: 3:378/388 of query aligns to 2:347/357 of 3ck5A
- active site: T19 (≠ F20), T50 (vs. gap), G137 (≠ T138), K164 (= K172), K166 (= K174), D195 (= D203), N197 (= N205), I220 (≠ F228), E221 (= E229), I243 (≠ V251), G246 (= G254), E247 (= E255), E268 (≠ Q276), D270 (= D278), H297 (= H305), G298 (= G310), V299 (≠ I311), Y315 (≠ F328), E317 (vs. gap)
- binding magnesium ion: D195 (= D203), E221 (= E229), E247 (= E255)
Q9RKF7 3,6-anhydro-alpha-L-galactonate cycloisomerase; AHGA cycloisomerase; EC 5.5.1.25 from Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
29% identity, 97% coverage: 3:378/388 of query aligns to 2:350/361 of Q9RKF7
- D195 (= D203) binding Mg(2+)
- E221 (= E229) binding Mg(2+)
- E247 (= E255) binding Mg(2+)
4h1zB Crystal structure of putative isomerase from sinorhizobium meliloti, open loop conformation (target efi-502104)
35% identity, 89% coverage: 31:376/388 of query aligns to 50:375/388 of 4h1zB
- active site: G70 (= G53), V72 (≠ A55), Y113 (≠ Q94), I157 (≠ A137), F180 (= F168), S182 (≠ L170), K184 (= K172), A186 (≠ K174), D214 (= D203), H216 (≠ N205), E240 (= E229), G265 (= G254), E266 (= E255), Q287 (= Q276), P288 (= P277), E289 (≠ D278), H315 (= H305), A316 (≠ V306), T317 (≠ A312), E341 (≠ L335)
- binding magnesium ion: D214 (= D203), E240 (= E229), E266 (= E255)
3stpA Crystal structure of a putative galactonate dehydratase
28% identity, 99% coverage: 1:386/388 of query aligns to 1:386/390 of 3stpA
- active site: L66 (≠ Y52), S69 (≠ A55), S151 (≠ T138), K177 (= K172), R179 (≠ K174), P189 (≠ L178), E214 (≠ D203), Y216 (≠ N205), E240 (= E229), G265 (= G254), E266 (= E255), H267 (≠ C256), Q287 (= Q276), D289 (= D278), I311 (≠ V300), H316 (= H305), E336 (= E342), F341 (≠ H347)
- binding magnesium ion: E214 (≠ D203), E240 (= E229), E266 (= E255)
Q8ZL58 L-talarate/galactarate dehydratase; TalrD/GalrD; StTGD; EC 4.2.1.156; EC 4.2.1.42 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
31% identity, 95% coverage: 11:380/388 of query aligns to 40:382/398 of Q8ZL58
- DAK 46:48 (≠ D-K 17:18) binding substrate
- KR 82:83 (vs. gap) binding substrate
- K195 (= K172) binding substrate
- K197 (= K174) active site, Proton acceptor; mutation to A: Loss of dehydration activity on both L-talarate and galactarate and loss of epimerization activity.
- D226 (= D203) binding Mg(2+)
- N228 (= N205) binding substrate
- E252 (= E229) binding Mg(2+)
- E278 (= E255) binding Mg(2+)
- H328 (= H305) active site, Proton donor/acceptor; mutation H->N,A: Loss of dehydration activity on both L-talarate and galactarate and loss of epimerization activity.
- E348 (= E342) binding substrate
2pp1A Crystal structure of l-talarate/galactarate dehydratase from salmonella typhimurium lt2 liganded with mg and l-lyxarohydroxamate (see paper)
31% identity, 95% coverage: 11:380/388 of query aligns to 37:379/395 of 2pp1A
- active site: K45 (= K18), S78 (vs. gap), K192 (= K172), K194 (= K174), D223 (= D203), N225 (= N205), E249 (= E229), G274 (= G254), E275 (= E255), D298 (= D278), H325 (= H305), E345 (= E342)
- binding (2r,3s,4r)-2,3,4-trihydroxy-5-(hydroxyamino)-5-oxopentanoic acid: D43 (= D17), K45 (= K18), K79 (vs. gap), F168 (= F146), K194 (= K174), E275 (= E255), H325 (= H305), E345 (= E342)
- binding magnesium ion: D223 (= D203), E249 (= E229), E275 (= E255)
4h19A Crystal structure of an enolase (mandelate racemase subgroup, target efi-502087) from agrobacterium tumefaciens, with bound mg and d- ribonohydroxamate, ordered loop
30% identity, 97% coverage: 1:377/388 of query aligns to 1:358/372 of 4h19A
- active site: I20 (≠ F20), T51 (≠ Q51), T143 (= T138), K172 (= K172), K174 (= K174), D203 (= D203), N205 (= N205), E229 (= E229), G254 (= G254), E255 (= E255), Q276 (= Q276), D278 (= D278), H305 (= H305), A306 (≠ S309), G307 (= G310), E327 (= E342)
- binding (2R,3R,4R)-N,2,3,4,5-pentakis(oxidanyl)pentanamide: D22 (≠ S22), H25 (≠ A25), H52 (≠ Y52), K172 (= K172), K174 (= K174), D203 (= D203), N205 (= N205), E229 (= E229), E255 (= E255), H305 (= H305), E327 (= E342)
- binding calcium ion: D268 (≠ A268), H298 (≠ W298)
- binding magnesium ion: D203 (= D203), E229 (= E229), E255 (= E255)
2pp3A Crystal structure of l-talarate/galactarate dehydratase mutant k197a liganded with mg and l-glucarate (see paper)
31% identity, 95% coverage: 11:380/388 of query aligns to 37:379/395 of 2pp3A
- active site: K45 (= K18), S78 (vs. gap), K192 (= K172), A194 (≠ K174), D223 (= D203), N225 (= N205), E249 (= E229), G274 (= G254), E275 (= E255), D298 (= D278), H325 (= H305), E345 (= E342)
- binding l-glucaric acid: D43 (= D17), K79 (vs. gap), K192 (= K172), D223 (= D203), N225 (= N205), E275 (= E255), H325 (= H305), E345 (= E342), F347 (≠ D344)
- binding magnesium ion: D223 (= D203), E249 (= E229), E275 (= E255)
2ggeD Crystal structure of mandelate racemase/muconate lactonizing enzyme from bacillus subtilis complexed with mg++ at 1.8 a
28% identity, 98% coverage: 9:388/388 of query aligns to 15:368/376 of 2ggeD
- active site: Y21 (≠ L15), S128 (≠ T138), K158 (= K172), K160 (= K174), D189 (= D203), N191 (= N205), E216 (= E229), G241 (= G254), E242 (= E255), D265 (= D278), H292 (= H305), N318 (= N331), D319 (≠ A332), E325 (= E342)
- binding magnesium ion: E216 (= E229), E242 (= E255), H292 (= H305)
Query Sequence
>WP_084931115.1 NCBI__GCF_002095475.1:WP_084931115.1
MKIKSVSSHLLRVPLGDKTFYSSQAAFPERNSYLVRIETDTGLVGWGEGGQYGPAQPVAA
VVDHVFAPRILGRDPTEPVRIWEALYAFSRDFGQRGTYIEALSAIDIALWDIAGQAAGLP
VWKLLGGRFRDKITAYATGCYYPEDFSHLPSQLTALHEEAAGYANAGFGLLKVKIGLLSL
EDDIARLRTIREAVGPTIGLLVDANHAYNAATAVRMGRMMKDLGVLFFEEPVVPEDREGY
RKVRAENPIAVAGGECEFTRYGFRDFIASGCVDIVQPDLAVCGGFTAFTQILTLANSWGV
STVPHVWGSGIAVAAALHAVATIPAFPFTANAIPLLNQPVIEFDRKHNPLRDDLLHQRFE
LIDGCLAVPDAPGLGVTVRDDILDQYTY
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory