SitesBLAST

Q9D6J6 NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial; NADH-ubiquinone oxidoreductase 24 kDa subunit; EC 7.1.1.2 from Mus musculus (Mouse) (see paper)
34% identity, 94% coverage: 12:171/171 of query aligns to 47:208/248 of Q9D6J6

query
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Q9D6J6

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C134 (= C97) binding [2Fe-2S] cluster
C139 (= C102) binding [2Fe-2S] cluster
C175 (= C138) binding [2Fe-2S] cluster
C179 (= C142) binding [2Fe-2S] cluster

7dgq9 NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (see paper)
33% identity, 94% coverage: 12:171/171 of query aligns to 9:170/207 of 7dgq9

query
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7dgq9

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binding fe2/s2 (inorganic) cluster: C96 (= C97), T98 (≠ S99), C101 (= C102), C137 (= C138), L138 (= L139), A140 (≠ N141), C141 (= C142)
binding : N20 (≠ E23), R23 (≠ A26), K29 (≠ H32), P32 (≠ E35), H35 (vs. gap), R49 (≠ K50)

P04394 NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial; NADH dehydrogenase subunit II; NADH-ubiquinone oxidoreductase 24 kDa subunit; EC 7.1.1.2 from Bos taurus (Bovine) (see 2 papers)
33% identity, 94% coverage: 12:171/171 of query aligns to 48:209/249 of P04394

query
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P04394

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C135 (= C97) binding [2Fe-2S] cluster
C140 (= C102) binding [2Fe-2S] cluster
C176 (= C138) binding [2Fe-2S] cluster
C180 (= C142) binding [2Fe-2S] cluster

Sites not aligning to the query:

1:32 modified: transit peptide, Mitochondrion

9fdkC Crystal structure of oxidized nuoef variant r66g(nuof) from aquifex aeolicus (see paper)
32% identity, 90% coverage: 17:170/171 of query aligns to 6:159/160 of 9fdkC

query
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9fdkC

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binding fe2/s2 (inorganic) cluster: C86 (= C97), V90 (= V101), C91 (= C102), C127 (= C138), L128 (= L139), A130 (≠ N141), C131 (= C142)

P19404 NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial; NDUFV2; NADH-ubiquinone oxidoreductase 24 kDa subunit; EC 7.1.1.2 from Homo sapiens (Human) (see 2 papers)
33% identity, 94% coverage: 12:171/171 of query aligns to 48:209/249 of P19404

query
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P19404

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Y193 (≠ H155) modified: Phosphotyrosine; by SRC

Sites not aligning to the query:

29 V → A: in dbSNP:rs906807

8oh5A Cryo-em structure of the electron bifurcating transhydrogenase stnabc complex from sporomusa ovata (state 2) (see paper)
36% identity, 83% coverage: 27:168/171 of query aligns to 6:147/150 of 8oh5A

query
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8oh5A

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binding fe2/s2 (inorganic) cluster: C76 (= C97), C81 (= C102), C117 (= C138), L118 (= L139), G119 (= G140), C120 (≠ N141), C121 (= C142)

8e9gE Mycobacterial respiratory complex i with both quinone positions modelled (see paper)
37% identity, 91% coverage: 13:168/171 of query aligns to 21:173/233 of 8e9gE

query
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8e9gE

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binding fe2/s2 (inorganic) cluster: C102 (= C97), C107 (= C102), C143 (= C138), N144 (≠ L139), A145 (≠ G140), A146 (≠ N141), C147 (= C142), V152 (≠ T147)

O52681 Bifurcating [FeFe] hydrogenase gamma subunit; Hydrogenase (NAD(+), ferredoxin) gamma subunit; Iron-hydrogenase gamma subunit; EC 1.12.1.4 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
30% identity, 87% coverage: 21:169/171 of query aligns to 2:150/161 of O52681

query
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O52681

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A

D

K

V

I

E

Y

G

G

V

V

A

A

T

T

F

F

Y

Y

S

A

Q

Q

I

F

Y

S

R

L

T

K

P

P

V

K

G

G

R

K

H

Y

V

T

I

I

R

M

Y

V

C

C

D
|
D

S
x
G

V
x
T

V
x
A

C

C

H

H

I

M

T

A

G

G

F

S

Q

P

G

E

I

V

Q

L

A

K

A

A

L

I

E

E

Q

E

N

E

L

T

N

G

I

L

K

T

P

P

G

G

Q

N

T

V

T

T

T

E

D

D

G

L

R

M

F

F

T

S

L

L

L

D

P

Q

T

V

C

G

C

C

L
|
L

G
|
G

N
x
A

C

C

D

A

K

L

G

A

P

P

T
x
V

M

M

M

V

V

I

D

N

E

G

D

E

T

V

H

Y

V

G

H

N

L

L

T

T

P

A

E

D

G

K

I

V

A

K

N

E

L

I

L

L

E

R

Q

K

D79 (= D98) mutation to P: Increase (30 mV) in the redox potential.
G80 (≠ S99) mutation to P: Large decrease (149 mV) in the redox potential. Causes a change to a [4Fe-4S] cluster protein.
T81 (≠ V100) mutation to P: Small increase (11 mV) in the redox potential.
A82 (≠ V101) mutation to P: Decrease (46 mV) in the redox potential.
L120 (= L139) mutation to P: Large decrease (65 mV) in the redox potential.
G121 (= G140) mutation to P: Decrease (22 mV) in the redox potential.
A122 (≠ N141) mutation to P: Large decrease (132 mV) in the redox potential.
V128 (≠ T147) mutation V->A,C,H,Y: Small increase (about 2-14 mV) in the redox potential.; mutation V->D,G,R,S,T,W: Increase (about 23-33 mV) in the redox potential.; mutation V->E,K,L,M: Decrease (about 37-50 mV) in the redox potential.; mutation V->F,I,P,Q: Small decrease (about 4-20 mV) in the redox potential.; mutation to N: Large increase (85 mV) in the redox potential.

7p5hC Tmhydabc- d2 map (see paper)
31% identity, 84% coverage: 27:169/171 of query aligns to 5:147/156 of 7p5hC

query
sites
7p5hC

I

V

E

E

H

E

E

I

K

L

H

K

Y

Y

E

G

D

Y

A

K

R

R

A

E

A

N

S

L

I

I

E

K

A

I

L

L

K

L

I

E

V

I

Q

Q

K

E

Q

I

R

Y

G

R

W

Y

V

L

P

P

D

E

G

D

A

V

I

I

N

N

A

Y

I

V

A

S

E

T

V

A

L

M

G

G

I

I

P

P

A

P

S

A

D

K

V

I

E

Y

G

G

V

V

A

A

T

T

F

F

Y

Y

S

A

Q

Q

I

F

Y

S

R

L

T

K

P

P

V

K

G

G

R

K

H

Y

V

T

I

I

R

M

Y

V

C
|
C

D

D

S
x
G

V

T

V
x
A

C
|
C

H

H

I

M

T

A

G

G

F

S

Q

P

G

E

I

V

Q

L

A

K

A

A

L

I

E

E

Q

E

N

E

L

T

N

G

I

L

K

T

P

P

G

G

Q

N

T

V

T

T

T

E

D

D

G

L

R

M

F

F

T

S

L

L

L

D

P

Q

T

V

C

G

C
|
C

L
|
L

G

G

N
x
A

C
|
C

D

A

K

L

G

A

P

P

T

V

M

M

M

V

V

I

D

N

E

G

D

E

T

V

H

Y

V

G

H

N

L

L

T

T

P

A

E

D

G

K

I

V

A

K

N

E

L

I

L

L

E

R

Q

K

binding fe2/s2 (inorganic) cluster: C75 (= C97), G77 (≠ S99), A79 (≠ V101), C80 (= C102), C116 (= C138), L117 (= L139), A119 (≠ N141), C120 (= C142)

8b6fAI ndutt15 (see paper)
36% identity, 81% coverage: 31:168/171 of query aligns to 34:173/231 of 8b6fAI

query
sites
8b6fAI

K

K

H

Y

H

P

Y

L

E

K

D

Q

A

K

R

R

A

S

A

A

S

V

I

M

E

P

A

L

L

L

K

Y

I

L

V

V

Q

Q

K

E

Q

Q

R

N

G

N

-

N

W

W

V

V

P

P

D

L

G

S

A

A

I

M

N

K

A

K

I

I

A

A

E

K

V

L

L

L

G

E

I

M

P

P

A

E

S

I

D

D

V

V

E

Y

G

E

V

V

A

A

T

T

F

F

Y

Y

S

T

Q

M

I

Y

Y

N

R

R

T

E

P

P

V

V

G

G

R

K

H

F

V

H

I

L

R

Q

Y

I

C
|
C

D

G

S

T

V

T

V
x
P

C
|
C

H

Q

I

L

T

C

G

G

F

S

Q

R

G

E

I

I

Q

T

A

K

A

A

L

I

E

E

Q

E

N

Y

L

T

N

Q

I

T

K

K

P

L

G

G

Q

H

T

T

T

S

T

A

D

D

G

G

R

K

F

W

T

T

L

L

L

E

P

E

T

V

C

E

C
|
C

L
|
L

G
|
G

N
x
A

C
|
C

D

S

K

N

G

A

P

P

T
x
M

M

I

M

Q

V

V

D

N

E

N

D

K

-

W

T

V

H

Y

V

E

H

D

L

L

T

T

P

T

E

E

G

N

I

V

A

V

N

K

L

L

E

K

binding fe2/s2 (inorganic) cluster: C101 (= C97), P105 (≠ V101), C106 (= C102), C142 (= C138), L143 (= L139), G144 (= G140), A145 (≠ N141), C146 (= C142), M151 (≠ T147)

7zmbH Cryoem structure of mitochondrial complex i from chaetomium thermophilum (state 2) (see paper)
32% identity, 91% coverage: 12:167/171 of query aligns to 14:173/221 of 7zmbH

query
sites
7zmbH

D

N

P

P

D

D

E

I

V

P

F

F

V

K

L

F

S

T

E

P

T

E

E

N

H

E

H

K

A

I

I

I

E

E

H

Q

-

I

-

L

E

K

K

R

H

Y

H

P

Y

P

E

Q

D

Y

A

K

R

K

A

A

S

V

I

M

E

P

A

L

L

L

K

D

I

L

V

G

Q

Q

K

R

Q

Q

R

H

G

G

W

F

V

C

P

S

D

I

G

S

A

V

I

M

N

N

A

E

I

V

A

A

E

R

V

I

L

L

G

E

I

M

P

P

A

P

S

M

D

R

V

V

E

Y

G

E

V

V

A

A

T

S

F

F

Y

Y

S

T

Q

M

I

Y

Y

N

R

R

T

T

P

P

V

V

G

G

R

K

H

F

V

H

I

V

R

Q

Y

A

C
|
C

D

T

S

T

V

T

V
x
P

C
|
C

H

Q

I

L

-

G

T

C

G

G

F

S

Q

D

G

A

I

I

Q

V

A

K

A

A

L

I

E

K

Q

E

N

H

L

L

N

G

I

I

K

N

P

Q

G

G

Q

E

T

T

T

P

D

D

G

G

R

L

F

F

T

T

L

F

L

I

P

E

T

V

C

E

C
|
C

L

L

G

G

N
x
A

C
|
C

D

V

K

N

G

A

P

P

T

M

M

V

M

Q

V

I

D

N

E

D

D

D

T

Y

H

Y

V

E

H

D

L

L

T

T

P

P

E

E

G

T

I

I

A

K

N

Q

L

V

L

L

binding fe2/s2 (inorganic) cluster: C101 (= C97), P105 (≠ V101), C106 (= C102), C144 (= C138), A147 (≠ N141), C148 (= C142)

8eswV2 NADH dehydrogenase (Ubiquinone) 24 kDa subunit, isoform A (see paper)
34% identity, 78% coverage: 38:171/171 of query aligns to 41:174/214 of 8eswV2

query
sites
8eswV2

R

R

A

G

A

A

S

M

I

I

E

P

A

L

L

L

K

D

I

L

V

A

Q

Q

K
x
R

Q

Q

R

Y

G

G

W

W

V

L

P

P

D

I

G

S

A

A

I

M

N

H

A

K

I

V

A

A

E

E

V

I

L

L

G

Q

I

L

P

P

A

N

S

M

D

R

V

V

E

Y

G

E

V

V

A

A

T

T

F

F

Y

Y

S

T

Q

M

I

F

Y

M

R

R

T

K

P

P

V

T

G

G

R

K

H

Y

V

H

I

I

R

Q

Y

V

C
|
C

D

T

S

T

V

T

V

P

C
|
C

H

W

I

L

T

R

G

G

F

S

Q

D

G

D

I

I

Q

L

A

E

A

T

L

C

E

K

Q

K

N

Q

L

L

N

G

I

I

K

G

P

V

G

G

Q

D

T

T

T

K

D

D

G

R

R

K

F

F

T

T

L

I

L

S

P

E

T

V

C

E

C
|
C

L
|
L

G
|
G

N

A

C
|
C

D

V

K

N

G

A

P

P

T

M

M

V

M

A

V

I

D

N

E

D

D

D

T

Y

H

Y

V

E

H

D

L

L

T

T

P

S

E

K

G

D

I

M

A

Q

N

D

L

I

L

L

E

N

Q

D

Y

L

Q

K

binding fe2/s2 (inorganic) cluster: C100 (= C97), C105 (= C102), C141 (= C138), L142 (= L139), G143 (= G140), C145 (= C142)
binding : R53 (≠ K50)

Sites not aligning to the query:

binding : 27, 30, 37

8b9zE Drosophila melanogaster complex i in the active state (dm1) (see paper)
34% identity, 78% coverage: 38:171/171 of query aligns to 41:174/214 of 8b9zE

query
sites
8b9zE

R

R

A

G

A

A

S

M

I

I

E

P

A

L

L

L

K

D

I

L

V

A

Q

Q

K

R

Q

Q

R

Y

G

G

W

W

V

L

P

P

D

I

G

S

A

A

I

M

N

H

A

K

I

V

A

A

E

E

V

I

L

L

G

Q

I

L

P

P

A

N

S

M

D

R

V

V

E

Y

G

E

V

V

A

A

T

T

F

F

Y

Y

S

T

Q

M

I

F

Y

M

R

R

T

K

P

P

V

T

G

G

R

K

H

Y

V

H

I

I

R

Q

Y

V

C
|
C

D

T

S
x
T

V
x
T

V
x
P

C
|
C

H

W

I

L

T

R

G

G

F

S

Q

D

G

D

I

I

Q

L

A

E

A

T

L

C

E

K

Q

K

N

Q

L

L

N

G

I

I

K

G

P

V

G

G

Q

D

T

T

T

K

D

D

G

R

R

K

F

F

T

T

L

I

L

S

P

E

T

V

C

E

C
|
C

L
|
L

G
|
G

N
x
A

C
|
C

D

V

K

N

G

A

P

P

T

M

M

V

M

A

V

I

D

N

E

D

D

D

T

Y

H

Y

V

E

H

D

L

L

T

T

P

S

E

K

G

D

I

M

A

Q

N

D

L

I

L

L

E

N

Q

D

Y

L

Q

K

binding fe2/s2 (inorganic) cluster: C100 (= C97), T102 (≠ S99), T103 (≠ V100), P104 (≠ V101), C105 (= C102), C141 (= C138), L142 (= L139), G143 (= G140), A144 (≠ N141), C145 (= C142)

8a5eC Cryo-em structure of the electron bifurcating fe-fe hydrogenase hydabc complex from acetobacterium woodii in the reduced state (see paper)
36% identity, 75% coverage: 42:170/171 of query aligns to 28:156/156 of 8a5eC

query
sites
8a5eC

I

M

E

Q

A

I

L

L

K

Q

I

E

V

T

Q

Q

K

L

Q

K

R

Y

G

G

W

Y

V

L

P

P

D

L

G

E

A

L

I

Q

N

G

A

T

I

I

A

A

E

D

V

E

L

L

G

G

I

I

P

P

A

L

S

T

D

E

V

V

E

Y

G

G

V

V

A

A

T

T

F

F

Y

Y

S

S

Q

Q

I

F

Y

T

R

L

T

K

P

P

V

K

G

G

R

K

H

Y

V

K

I

I

R

G

Y

I

C
|
C

D

L

S
x
G

V

T

V
x
A

C
|
C

H

Y

I

V

T

R

G

G

F

S

Q

Q

G

A

I

I

Q

I

A

D

A

K

L

V

E

N

Q

S

N

V

L

L

N

G

I

T

K

Q

P

V

G

G

Q

D

T

T

T

E

D

D

G

G

R

K

F

W

T

S

L

V

L

D

P

A

T

T

C
x
R

C
|
C

L

V

G

G

N

A

C
|
C

D

G

K

L

G

A

P

P

T

V

M

M

V

I

D

N

E

E

D

E

T

V

H

F

V

G

H

R

L

L

T

T

P

V

E

D

G

E

I

I

A

P

N

G

L

I

L

L

E

E

Q

K

Y

Y

binding fe2/s2 (inorganic) cluster: C83 (= C97), G85 (≠ S99), A87 (≠ V101), C88 (= C102), R123 (≠ C137), C124 (= C138), C128 (= C142)

Query Sequence

>WP_084934605.1 NCBI__GCF_002095475.1:WP_084934605.1
MHDQKIAIHTIDPDEVFVLSETEHHAIEHEKHHYEDARAASIEALKIVQKQRGWVPDGAI
NAIAEVLGIPASDVEGVATFYSQIYRTPVGRHVIRYCDSVVCHITGFQGIQAALEQNLNI
KPGQTTTDGRFTLLPTCCLGNCDKGPTMMVDEDTHVHLTPEGIANLLEQYQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory