SitesBLAST

T27 (= T38) mutation T->A,S: Retains 60% of wild-type activity.; mutation to N: Retains 20% of wild-type activity.
E98 (≠ C109) mutation to A: Retains 80% of wild-type activity.
K156 (= K167) mutation K->A,Q: Retains less than 10% of wild-type activity.; mutation to R: Retains 40% of wild-type activity.
F212 (= F222) mutation F->A,Q: Loss of activity.; mutation to Y: No change in activity.
E218 (= E228) mutation E->A,Q: Loss of activity.; mutation to D: Retains 70% of wild-type activity.

P15993 Aromatic amino acid transport protein AroP; Aromatic amino acid:H(+) symporter AroP; General aromatic amino acid permease; General aromatic transport system from Escherichia coli (strain K12) (see paper)
39% identity, 98% coverage: 9:463/463 of query aligns to 1:451/457 of P15993

query
sites
P15993

L

M

I

M

E

E

A

G

E

Q

Q

Q

P

H

-

G

E

E

K

Q

L

L

R

K

R

R

N

G

L

L

H

K

N

N

R

R

H

H

I

I

Q

Q

L

L

I

I

A

A

I

L

G

G

A

A

I

I

G

G

T

T

G

G

L

L

F

F

M

L

G

G

S

S

G

A

K

S

T

V

I

I

S

Q

L

S

A

A

G

G

P

P

S

G

I

I

F

L

V

G

Y

Y

M

A

I

I

I

A

G

G

F

F

M

I

L

A

F

F

F

L

V

I

M

M

R

R

A

Q

M

L

G

G

E

E

L

M

L

V

S

E

N

E

L

P

E

V

Y

A

K

G

S

S

F

F

S

S

D

H

F

F

A

A

A

Y

D

K

L

Y

L

W

G

G

P

S

W

F

A

A

G

G

Y

F

F

A

T

S

G

G

W

W

T

N

Y

Y

W

W

F

V

C

L

W
x
Y

V

V

V

L

T

V

G

A

I

M

A

A

D

E

V

L

V

T

A

A

I

V

S

G

A

K

Y

Y

F

I

Q

Q

L

F

W

W

F

Y

P

P

G

E

F

I

S

P

I

T

W

W

M

V

S

S

A

A

L

A

L

V

C

F

I

F

F

V

A

V

F

I

L

N

A

A

L

I

N

N

I

L

A

T

T

N

V

V

K

K

L

V

F

F

G

G

E

E

M

M

E

E

F

F

W

W

F

F

A

A

I

I

K

K

I

V

V

I

A

A

I

V

V

V

A

A

L

M

I

I

T

F

G

G

I

G

V

W

L

L

V

L

S

-

M

-

H

-

Y

F

P

S

S

G

P

N

N

G

G

G

T

P

T

Q

A

A

S

T

L

V

S

S

N

N

I

L

W

W

D

D

H

Q

G

G

M

F

F

L

P

P

K

H

G

G

L

F

S

T

G

G

F

L

F

V

A

M

G

M

F

M

Q

A

I

I

A

I

V

M

F

F

A

S

F

F

V

G

G

G

I

L

E

E

L

L

V

V

G

G

T

I

A

T

A

A

E

E

T

A

K

D

D

N

P

P

H

E

K

Q

V

S

L

I

P

P

R

K

A

A

I

T

N

N

A

Q

I

V

P

I

V

Y

R

R

I

I

I

L

M

I

F

F

Y

Y

V

I

L

G

A

S

L

L

L

A

V

V

I

L

M

L

A

S

V

L

T

M

P

P

W

W

N

T

Q

R

V

V

L

T

P

A

N

D

R

T

S

S

P

P

F

F

V

V

E

L

M

I

F

F

V

H

L

E

I

L

G

G

L

D

P

T

A

F

A

V

A

A

S

N

I

A

V

L

N

N

F

I

V

V

L

L

T

T

S

A

A

A

A

L

S

S

S

V

A

Y

N

N

S

S

G

C

I

V

F

Y

S

C

T

N

S

S

R

R

M

M

L

L

F

F

G

G

L

L

A

A

E

Q

L

Q

G

G

V

N

A

A

H

P

K

K

A

A

F

L

G

A

R

S

L

V

S

D

A

K

R

R

A

G

V

V

P

P

T

V

T

N

G

T

L

I

F

L

F

V

S

S

C

A

L

L

C

V

L

T

L

A

V

L

G

C

V

V

A

L

L

I

I

N

Y

Y

L

L

I

A

P

P

D

E

V

S

M

A

T

F

V

G

F

L

T

L

M

M

V

A

T

L

T

V

V

V

S

S

A

A

I

L

L

V

F

I

M

N

F

-

V

-

W

W

T

A

I

M

I

I

L

S

C

L

S

A

Y

H

L

M

A

K

Y

F

R

R

K

R

Q

A

H

K

P

Q

Q

E

R

Q

H

G

A

V

S

T

K

R

F

F

K

P

M

A

P

L

L

L

G

Y

K

P

F

L

M

G

C

N

W

W

V

I

C

C

M

L

A

L

F

F

F

M

A

A

F

A

V

V

L

L

V

V

L

I

L

M

T

L

L

M

Q

T

E

P

D

G

T

M

R

A

Q

I

A

S

L

V

M

Y

V

L

T

I

P

P

L

V

W

W

F

L

V

I

I

V

L

L

T

G

L

I

G

G

W

Y

L

L

L

F

R

K

R

E

R

K

K

T

A

A

Y103 (≠ W110) Key residue for tryptophan transport; mutation to F: Decreases tryptophan transport to less than 50% of wild-type levels and reduces the ability of tryptophan to inhibit phenylalanine transport from 95 to 62%.

P24207 Phenylalanine-specific permease; Phenylalanine:H(+) symporter PheP from Escherichia coli (strain K12) (see 3 papers)
39% identity, 97% coverage: 12:462/463 of query aligns to 13:458/458 of P24207

query
sites
P24207

A

S

E

N

Q

Q

P

E

E

P

K

T

L

L

R

H

R

R

N

G

L

L

H

H

N

N

R
|
R

H

H

I

I

Q

Q

L

L

I

I

A

A

I

L

G

G

A

A

I

I

G

G

T

T

G

G

L

L

F

F

M

L

G

G

S

I

G

G

K

P

T

A

I

I

S

Q

L

M

A

A

G

G

P
|
P

S

A

I

V

I

L

F

L

V

G

Y

Y

M

G

I

V

I

A

G

G

F

I

M

I

L

A

F

F

F

L

V

I

M

M

R

R

A

Q

M

L

G

G

E

E

L

M

L

V

S

E

N

E

L

P

E

V

Y

S

K

G

S

S

F
|
F

S

A

D

H

F
|
F

A

A

A
x
Y

D

K

L
x
Y

L
x
W

G

G

P

P

W
x
F

A

A

G

G

Y
x
F

F

L

T

S

G

G

W
|
W

T

N

Y
|
Y

W
|
W

F

V

C

M

W
x
F

V

V

V

L

T

V

G

G

I

M

A

A

D
x
E

V

L

V

T

A

A

I

A

S

G

A

I

Y

Y

F

M

Q

Q

L

Y

W

W

F

F

P

P

G

D

F

V

S

P

I

T

W

W

M

I

S

W

A

A

L

A

C

F

I

F

F

I

A

I

F

I

L

N

A

A

L

V

N

N

I

L

A

V

T

N

V

V

K

R

L

L

F

Y

G

G

E

E

M

T

E

E

F

F

W

W

F

F

A

A

I

L

I

I

K
|
K

I

V

V

L

A

A

I

I

V

I

A

G

L

M

I

I

I

G

T

F

G

G

I

L

V

W

L

L

V

L

S

-

M

-

H

-

Y

F

P

S

S

G

P

H

N

G

G

G

T

E

T

K

A

A

S

S

L

I

S

D

N

N

I

L

W

W

D

R

H

Y

G

G

M

F

F

F

P

A

K

T

G

G

L

W

S

N

G

G

F

L

F

I

A

L

G

S

F

L

Q

A

I

V

A

I

V

M

F

F

A

S

F

F

V

G

G

G

I

L

E
|
E

L

L

V

I

G

G

T

I

A

T

A

A

E

E

T

A

K

R

D

D

P

P

H

E

K

K

V

S

L

I

P

P

R

K

A

A

I

V

N

N

A

Q

I

V

P

V

V

Y

R
|
R

I

I

I

L

M

L

F

F

Y

Y

V

I

L

G

A

S

L

L

L

V

V

V

I

L

M

L

A

A

V

L

T

Y

P

P

W

W

N

V

Q

E

V

V

L

K

P

S

N

N

R

S

S

S

P

P

F

F

V

V

E

M

M

I

F

F

V

H

L

N

I

L

G

D

L

S

P

N

A

V

A

A

S

S

I

A

V

L

N

N

F

F

V

V

V

I

L

L

T

V

S

A

A

S

A

L

S

S

S

V

A

Y

N

N

S

S

G

G

I

V

F

Y

S

S

T

N

S

S

R

R

M

M

L

L

F

F

G

G

L

L

A

S

E

V

L

Q

G

G

V

N

A

A

H

P

K

K

A

F

F

L

G

T

R

R

L

V

S

S

A

R

R

R

A

G

V

V

P
|
P

T

I

T

N

G

S

L

L

F

M

F

L

S

S

C

G

L

A

C

I

L

T

L

S

V

L

G

V

V

V

A

L

L

I

I

N

Y

Y

L

L

I

L

P

P

D

Q

V

K

M

A

T

F

V

G

F

L

T

L

M

M

V

A

T

L

T

V

V

V

S

A

A

T

I

L

L

L

F

L

M

N

F

-

V

-

W

W

T

I

I

M

I

I

L

C

C

L

S

A

Y

H

L

L

A

R

Y

F

R

R

K

A

Q

A

H

M

P

R

Q

R

R

Q

H

G

A

R

A

E

S

T

K

Q

F

F

K

K

M

A

P

L

L

L

G

Y

K

P

F

F

M

G

C

N

W

Y

V

L

C

C

M

I

A

A

F

F

F

L

A

G

F

M

V

I

L

L

V

L

L

L

L

M

T

C

L

T

Q

M

E

D

D

D

T

M

R

R

Q

L

A

S

L

A

M

I

V

L

T

L

P
|
P

L

V

W

W

F

I

V

V

I

F

L

L

T

F

L

M

G

A

W

F

L

K

L

T

R

L

R

R

K

K

R26 (= R25) mutation R->G,S,Q: Strong decrease in phenylalanine transport activity.
P54 (= P53) mutation to A: 50% of wild-type phenylalanine transport activity.; mutation to G: No change in phenylalanine transport activity.; mutation to L: 26% of wild-type phenylalanine transport activity.
F87 (= F86) mutation to L: No effect on phenylalanine transport activity.
F90 (= F89) mutation to L: 65% of wild-type phenylalanine transport activity.
Y92 (≠ A91) mutation to L: 41% of wild-type phenylalanine transport activity.
Y94 (≠ L93) mutation to L: 69% of wild-type phenylalanine transport activity.
W95 (≠ L94) mutation to L: 10% of wild-type phenylalanine transport activity.
F98 (≠ W97) mutation to L: No effect on phenylalanine transport activity.
F101 (≠ Y100) mutation to L: 38% of wild-type phenylalanine transport activity.
W105 (= W104) mutation to L: 39% of wild-type phenylalanine transport activity.
Y107 (= Y106) mutation to L: No effect on phenylalanine transport activity.
W108 (= W107) mutation to L: 71% of wild-type phenylalanine transport activity.
F111 (≠ W110) mutation to L: 60% of wild-type phenylalanine transport activity.; mutation to Y: Enables the transport of tryptophan to almost the same steady-state level as that of phenylalanine.
E118 (≠ D117) mutation E->G,L,V,N: Loss of activity.
K168 (= K167) mutation K->L,R: Strong decrease in phenylalanine transport activity.; mutation to N: Loss of activity.
E226 (= E228) mutation E->A,Q,K,R,W: Loss of activity.
R252 (= R254) mutation R->D,E,F,W,P: Loss of activity.
P341 (= P343) mutation to A: 5% of wild-type phenylalanine transport activity.; mutation P->G,Q,K,R: Loss of activity.; mutation to S: 3% of wild-type phenylalanine transport activity.; mutation to T: 17% of wild-type phenylalanine transport activity.
P442 (= P446) mutation to A: 46% of wild-type phenylalanine transport activity.; mutation to G: 52% of wild-type phenylalanine transport activity.; mutation to L: 43% of wild-type phenylalanine transport activity.

P46349 Gamma-aminobutyric acid permease; GABA permease; 4-aminobutyrate permease; Gamma-aminobutyrate permease; Proline transporter GabP from Bacillus subtilis (strain 168) (see paper)
35% identity, 97% coverage: 13:462/463 of query aligns to 3:450/469 of P46349

query
sites
P46349

E

Q

Q

S

P

Q

E

S

K

G

L

L

R

K

N

E

L

L

H

K

N

T

R

R

H

H

I

M

Q

T

L

M

I

I

A

S

I

I

G

A

G

G

A

V

I

I

G

G

T

A

G

G

L

L

F

F

M

V

G
|
G

S

S

G

G

K

S

T

V

I

I

S

H

L

S

A

T

G
|
G

P

P

S

G

I

A

I

V

F

V

V

S

Y

Y

M

A

I

L

I

A

G

G

F

L

M

L

L

V

F

I

F

F

V

I

M

M

R

R

A

M

M

L

G

G

E

E

L

M

L

S

L

A

S

V

N

N

L

P

E

T

Y

S

K

G

S

S

F

F

S

S

D

Q

F

Y

A

A

A

H

D

D

L

A

L

I

G

G

P

P

W

W

A

A

G

G

Y

F

F

T

T

I

G

G

W

W

T

L

Y

Y

W

W

F

F

C

F

W

W

V

V

V

I

T

V

G

I

I

A

A

I

D

E

V

A

V

I

A

A

I

G

S

A

A

G

Y

I

F

I

Q

Q

L

Y

W

W

F

F

P

H

G

D

F

I

S

P

I

L

W

W

M

L

S

T

A

S

L

L

L

I

C

L

I

T

F

I

A

V

F

L

L

T

A

L

L

T

N

N

I

V

A

Y

T

S

V

V

K

K

L

S

F

F

G

G

E

E

M

F

E

E

F

Y

W

W

F

F

A

S

I

L

I

I

K

K

I

V

V

V

A

T

I

I

V

I

A

A

L

F

I

L

I

I

T

V

G

G

I

F

V

A

L

F

V

I

S

F

M

G

H

F

Y

-

P

-

S

A

P

P

N

G

G

S

T

E

T

P

A

V

S

G

L

F

S

S

N

N

I

L

W

T

D

G

H

K

G

G

M

F

F

F

P

P

K

E

G

G

L

I

S

S

G

S

F

V

F

L

A

L

G

G

F

I

Q

V

I

V

A

V

V

I

F

F

A

S

F

F

V

M

G

G

I

T

E

E

L

I

V

V

G

A

T

I

A

A

A

G

E

E

T

T

K

S

D

N

P

P

H

I

K

E

V

S

L

V

P

T

R

K

A

A

I

T

N

R

A

S

I

V

P

V

V

W

R

R

I

I

M

V

F

F

Y

Y

V

V

L

G

A

S

L

I

L

A

V

I

I

V

M

V

A

A

V

L

T

L

P

P

W

W

N

N

Q

S

V

A

L

N

P

I

N

L

R

E

S

S

P

P

F

F

V

V

E

A

M

V

F

L

V

E

L

H

I

I

G

G

L

V

P

P

A

A

S

Q

I

I

V

M

N

N

F

F

V

I

V

V

L

L

T

T

S

A

A

V

A

L

S

S

S

C

A

L

N

N

S

S

G
|
G

I

L

F

Y

S

T

T

T

S

S

R

R

M

M

L

L

F

Y

G

S

L

L

A

A

E

E

L

R

G

N

V

E

A

A

H

P

K

R

A

R

F

F

G

M

R

K

L

L

S

S

A

K

R

K

A

G

V

V

P

P

T

V

T

Q

G

A

L

I

F

V

F

A

S
x
G

C

T

L

F

C
x
F

L

S

L

Y

V

I

G

A

V

V

A

V

L

M

I

N

Y

Y

L

F

I

S

P

P

D

D

V

-

M

-

T

T

V

V

F

F

T

L

M

F

V

L

T

V

T

N

V

S

S

S

A

G

I

A

L

I

F

A

M

L

F

L

V

V

W

Y

T

L

I

V

I

I

L

A

C

V

S

S

Y

Q

L

L

A

K

Y

M

R

R

K

K

Q

K

H

L

P

E

Q

K

R

T

H

N

-

P

A

E

A

A

S

L

K

K

F

I

K

K

M

M

P

W

L

L

G

F

K

P

F

F

M

L

C

T

W

Y

V

L

C

T

M

I

A

I

F

A

F

I

A

C

F
x
G

V

I

L

L

V

V

L

S

L

M

T

A

L

F

Q

I

E

D

D

S

T

M

R

R

Q

D

A

E

L

L

M

L

V

L

T

T

P

G

L

V

W

I

F

T

V

G

I

I

L

V

T

L

L

I

G

S

W

Y

L

L

L

V

-

F

R

R

R

K

R

R

K

K

G33 (= G43) mutation to D: Lack of activity.
G42 (= G52) mutation to S: Lack of activity.
G301 (= G313) mutation to V: Lack of activity.
G338 (≠ S350) mutation to E: Lack of activity.
F341 (≠ C353) mutation to S: Lack of activity.
G414 (≠ F427) mutation to R: Lack of activity.

P25737 Lysine-specific permease LysP; Lysine transporter LysP; Trigger transporter LysP from Escherichia coli (strain K12) (see 2 papers)
34% identity, 97% coverage: 1:448/463 of query aligns to 1:447/489 of P25737

query
sites
P25737

M
|
M

V

V

D

S

Q

E

S

T

K

K

E

T

A

T

L

-

I

-

E

-

A

-

E

E

Q

A

P

P

E

-

K

G

L

L

R

R

N

E

L

L

H

K

N

A

R

R

H

H

I

L

Q

T

L

M

I

I

A

A

I

I

G

G

A

S

I

I

G

G

T

T

G

G

L

L

F

F

M

V

G

A

S

S

G

G

K

A

T

T

I

I

S

S

L

Q

A

A

G

G

P

P

S

G

-

G

I

A

I

L

F

L

V

S

Y

Y

M

M

I

L

I

I

G

G

F

L

M

M

L

V

F

Y

F

F

V

L

M

M

R

T

A

S

M

L

G

G

E

E

L

L

L

A

S

Y

N

M

L

P

E

V

Y

S

K

G

S

S

F

F

S

A

D

T

F

Y

A

G

A

Q

D

N

L

Y

L

V

G

E

P

E

W

G

A

F

G

G

Y

F

F

A

T

L

G

G

W

W

T

N

Y
|
Y

W

W

F

Y

C

N

W
|
W

V

A

V

V

T

T

G

I

I

A

A

V

D

D

V

L

V

V

A

A

I

A

S

Q

A

L

Y

V

F

M

Q

S

L

W

W

W

F

F

P

P

G

D

F

T

S

P

I

G

W

W

M

I

S

W

A

S

L

A

L

L

C

F

I

L

F

G

A

V

F

I

L

F

A

L

L

L

N

N

I

Y

A

I

T

S

V

V

K

R

L

G

F

F

G

G

E

E

M

A

E

E

F

Y

W

W

F

F

A

S

I

L

I

I

K
|
K

I

V

V

T

A

T

I

V

V

I

A

V

L

F

I

I

T

V

G

G

I

V

V

L

L

M

V

I

S

I

M

G

H

I

Y

F

P

K

S

G

P

A

N

Q

G

-

T

-

T

P

A

A

S

G

L

W

S

S

N

N

I

-

W

W

D

T

H

I

G

G

-

E

G

A

M

P

F

F

P

A

K

G

G

G

L

F

S

A

G

A

F

M

F

I

A

G

G

V

F

A

Q

M

I

I

A

V

V

G

F
|
F

A

S

F

F

V

Q

G

G

I

T

E
|
E

L

L

V

I

G

G

T

I

A

A

A

G

E
|
E

T

S

K

E

D

D

P

P

H

A

K

K

V

N

L

I

P

P

R

R

A

A

I

V

N

R

A

Q

I

V

P

F

V

W

R

R

I

I

I

L

M

L

F

F

Y

Y

V

V

L

F

A

A

L

I

L

L

V

I

I

I

M

S

A

L

V

I

T

I

P

P

W

Y

N

T

Q

D

-

P

-

S

V

L

L

L

P

R

N

N

R
x
D

-

V

-

K

-
x
D

-

I

-

S

-

V

S

S

P

P

F

F

V

T

E

L

M

V

F

F

V

Q

L

H

I

A

G

G

L

L

P

L

A

S

A

A

S

A

I

V

V

M

N

N

F

A

V

V

V

I

L

L

T

T

S

A

A

V

A

L

S

S

S

A

A

G

N

N

S

S

G

G

I

M

F

Y

S

A

T

S

S

T

R

R

M

M

L

L

F

Y

G

T

L

L

A

A

E

C

L

D

G

G

V

K

A

A

H

P

K

R

A

I

F

F

G

A

R

K

L

L

S

S

A

R

R

G

A

G

V

V

P

P

T

R

T

N

G

A

L

L

F

Y

F

-

S

-

C

A

L

T

C

T

L

V

L

I

V

A

G

G

V

L

A

C

L

F

I

L

Y

T

L

S

I

M

P

F

D

G

V

N

M

Q

T

T

V

V

F

Y

T

L

M

W

V

L

T

L

T

N

V

T

S

S

A

G

I

M

L

T

F

G

M

F

F

I

V

A

W

W

T

L

I

G

I

I

L

A

C

I

S

S

Y

H

L

Y

A

R

Y

F

R

R

K

R

Q

G

H

Y

P

V

-

L

Q

Q

R

G

H

H

A

D

A

I

S

N

K

D

F

L

K

P

M

Y

P

R

L

S

G

G

K

F

F

F

M

-

C

-

W

-

V

P

C

L

M

G

A

P

F

I

F

F

A

A

F

F

V

I

L

L

V

C

L

L

-

I

L

I

T

T

L

L

Q

G

E

Q

D

N

T

Y

R
x
E

Q

A

A

F

L

L

M

K

V
x
D

T

T

P

I

L
x
D

W

W

M1 (= M1) modified: Initiator methionine, Removed
Y102 (= Y106) mutation to L: Retains 4% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
W106 (= W110) mutation to L: Retains 20% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
K163 (= K167) mutation to A: Retains 24% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
F216 (= F222) mutation to L: Retains 13% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
E222 (= E228) mutation to A: Abolishes lysine uptake. Strongly inhibits CadC.
E230 (= E236) mutation to V: Abolishes lysine uptake. Shows significant less inhibition of CadC.
D275 (≠ R279) Essential for the stimulus-dependent interaction with CadC; mutation to A: Retains 88% of wild-type lysine uptake activity, but can hardly inhibit CadC. Cannot interact with CadC; when associated with A-278.
D278 (vs. gap) Essential for the stimulus-dependent interaction with CadC; mutation to A: Retains 88% of wild-type lysine uptake activity, but can hardly inhibit CadC. Cannot interact with CadC; when associated with A-275.
E438 (≠ R439) mutation to A: Retains 14% of wild-type lysine uptake activity. Is unable to inhibit CadC.
D443 (≠ V444) mutation to A: Retains 11% of wild-type lysine uptake activity. Is unable to inhibit CadC.
D446 (≠ L447) mutation to A: Retains 13% of wild-type lysine uptake activity. Is unable to inhibit CadC.

Q9URZ4 Cationic amino acid transporter 1 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
32% identity, 93% coverage: 3:432/463 of query aligns to 65:502/587 of Q9URZ4

query
sites
Q9URZ4

D

D

Q

D

S

F

K

K

E

P

A

A

L

K

I

A

E

T

A

G

E

E

Q

D

P

G

E

T

K

A

L

L

R

K

R

R

N

S

L

L

H

K

N

S

R

R

H

H

I

M

Q

Q

L

M

I

I

A

S

I

I

G

G

A

A

I

I

G

G

T

T

G

G

L

L

F

Y

M

V

G

G

S

S

G

G

K

S

T

S

I

L

S

A

L

D

A

G

G

G

P

P

-

A

S

S

I

V

I

I

F

I

V

N

Y

Y

M

S

I

L

I

I

G

G

F

I

M

M

L

M

F

F

V

I

M

V

R

Y

A

A

M

L

G

G

E

E

L

M

L

A

L

V

S

A

N

Y

L

P

E

V

Y

A

K

G

S

G

F

F

S

N

D

T

F

Y

A

A

A

T

D

R

L

F

L

I

G

D

P

P

W

A

A

W

G

G

Y

F

F

A

T

V

G

S

W

W

T

N

Y

Y

W

F

F

I

C

N

W

Y

V

F

V

V

T

T

G

F

I

P

A

L

D

E

V

L

V

T

A

T

I

C

S

A

A

I

Y

T

F

F

Q

R

L

Y

W

W

F

T

P

D

-

I

G

N

F

S

S

A

I

A

W

W

M

I

S

S

A

I

L

F

L

L

C

V

I

V

F

I

A

-

F

-

L

I

A

I

L

V

N

N

I

L

A

F

T

G

V

V

K

R

L

A

F

Y

G

G

E

E

M

V

E

E

F

F

W

I

F

L

A

S

I

T

I

V

K

K

I

V

V

V

A

A

I

T

V

F

A

G

L

F

I

I

T

L

G

A

I

I

V

I

L

I

V

N

S

C

M

G

H

G

Y

V

P

P

S

T

P

D

N

H

G

R

T

G

T

Y

A

I

S

G

L

G

S

S

N

-

I

I

W

I

D

K

H

H

G

K

G

-

M

P

F

F

P

R

K

H

G

G

L

F

S

K

G

G

F

F

F

C

A

S

G

V

F

F

Q

T

I

T

A

A

V

A

F

F

A

S

F

F

V

S

G

G

I

T

E

E

L

V

V

I

G

G

T

L

A

A

E

E

T

V

K

D

D

N

P

P

H

Q

K

K

V

A

L

L

P

P

R

H

A

A

I

V

N

K

A

Q

I

V

P

F

V

W

R

R

I

I

I

A

M

I

F

F

Y

Y

V

V

L

V

A

S

L

L

L

I

V

L

I

I

-

G

M

L

A

L

V

I

T

S

P

P

W

D

N

D

-

P

Q

N

V

L

L

M

P

G

N

N

R

G

S

S

P

T

F

S

V

V

E

S

M

P

F

F

V

V

L

L

I

A

-

I

-

K

-

E

-

A

-

N

-

I

-

K

G

G

L

L

P

P

A

-

S

S

I

V

V

F

N

N

F

A

V

V

V

I

L

I

T

I

S

S

A

V

S

S

S

V

A

T

N

N

S

S

G

S

I

T

F

Y

S

T

T

A

S

G

R

R

M

T

L

L

F

H

G

G

L

M

A

A

E

N

L

L

G

K

V

Q

A

A

H

P

K

S

A

F

F

F

G

K

R

Y

L

T

S

D

A

R

R

L

A

G

V

R

P

P

T

-

G

-

L

L

F

L

F

A

S

M

C

I

L

V

C

V

L

L

V

F

G

G

V

F

A

F

L

A

I

Y

Y

I

L

N

I

E

P

A

D

D

V

K

M

N

-

G

-

N

-

D

-

V

-

S

-

D

T

T

V

V

F

F

T

N

M

W

V

L

T

L

T

A

V

L

S

S

A

G

I

L

L

S

F

N

M

F

F

F

V

T

W

W

-

G

T

S

I

I

I

C

L

L

C

C

S

H

-

I

-

F

Y

R

L

L

A

A

Y

F

R

K

K

K

Q

Q

-

G

H

H

P

S

Q

L

R

K

H

E

A

L

A

G

S

-

K

-

F

F

K

V

M

S

P

P

L

M

G

G

K

-

F

-

M

-

C

I

W

W

-

G

V

S

C

C

M

I

A

G

F

L

F

F

A

F

F

N

V

I

L

L

V

C

L

L

L

M

Sites not aligning to the query:

29 modified: Phosphoserine
30 modified: Phosphoserine
37 modified: Phosphoserine

P04817 Arginine permease CAN1; Canavanine resistance protein 1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
30% identity, 92% coverage: 11:435/463 of query aligns to 76:513/590 of P04817

query
sites
P04817

E

E

A

G

E

E

-

V

Q

Q

P

N

E

A

K

E

L

V

R

K

R

R

N

E

L

L

H

K

N

Q

R

R

H

H

I

I

Q

G

L

M

I

I

A

A

I

L

G

G

A

T

I

I

G

G

T

T

G

G

L

L

F

F

M

I

G

G

S

L

G

S

K

T

T
x
P

I

L

S

T

L

N

A

A

G

G

P

P

-

V

S

G

I

A

I

L

F

I

V

S

Y

Y

M

L

I

F

I

M

G

G

F

S

M

L

L

A

F

Y

F

S

V

V

M

T

R

Q

A

S

M

L

G

G

E

E

L

M

L

A

L

T

S

F

N

I

L
x
P

E
x
V

Y

T

K

S

S
|
S

F

F

S

T

D

V

F

F

A

S

A

Q

D

R

L

F

L

L

G

S

P

P

W

A

A

F

G

G

Y

A

F

A

T

N

G

G

W

Y

T

M

Y
|
Y

W

W

F

F

C

S

W

W

V

A

V

I

T

T

G

F

I

A

A

L

D

E

V

L

V

S

A

V

I

V

S

G

A

Q

Y

V

F

I

Q

Q

L

F

W

W

F

-

P

-

G

T

F

Y

S

K

I

V

W

P

M

L

S

A

A

A

L

W

L

I

C

S

I

I

F

F

A

W

F

V

L

I

A

I

-

T

-

I

L

M

N

N

I

L

A

F

T

P

V

V

K

K

L

Y

F

Y

G

G

E

E

M

F

E

E

F

F

W

W

F

V

A

A

I

S

I

I

K

K

I

V

V

L

A

A

I

I

V

I

A

G

L

F

I

L

I

I

T

Y

G

C

I

F

V

C

L

M

V

V

S

C

M

G

H

A

Y

-

P

-

S

-

P

-

N

-

G

G

T

V

T

T

A

G

S

P

L

V

S

G

-

F

N

R

I

Y

W

W

D

R

H

N

G

P

G

G

M

A

F

W

P

G

K

P

G

G

L

I

-

I

-

S

-

K

-

D

-

K

-

N

S

E

G

G

F

R

F

F

A

L

G

G

F

W

Q

V

I

S

-

S

-

L

-

I

-

N

A

A

V

A

F

F

A

T

F

F

V

Q

G

G

I

T

E

E

L

L

V

V

G

G

T

I

A

T

A

A

A
x
G

E

E

T

A

K

A

D

N

P
|
P

H

R

K

K

V

S

L

V

P

P

R

R

A

A

I

I

N

K

A

K

I

V

P

V

V

F

R

R

I

I

I

L

M

T

F

F

Y

Y

V

I

L

G

A

S

L

L

V

F

I

I

M

G

A

L

V

L

T

V

P

P

W

Y

N

N

Q

D

-

P

-

K

-

L

-

T

-

Q

-

S

-
x
T

V
x
S

L
x
Y

P

V

N

S

R

T

S

S

P

P

F

F

V

I

E

I

M

A

F

I

V

E

L

N

I

S

G

G

L

T

P

K

A

V

A

L

A

P

S

H

I

I

V

F

N

N

F

A

V

V

V

I

L

L

T

T

S

T

A

I

S

S

S

A

A

A

N

N

S

S

G

N

I

I

F

Y

S

V

T

G

S

S

R

R

M

I

L

L

F

F

G

G

L

L

A

S

E

K

L

N

G

K

V

L

A

A

H

P

K

K

A

F

F

L

G

S

R

R

L

T

S

T

A

K

R

G

A

G

V

V

P

P

T

Y

T

I

G

A

L

V

F

F

F

V

S

T

C

A

L

A

C

F

L

-

V

-

G

-

V

G

A

A

L

L

I

A

Y

Y

L

M

I

E

P

T

D

S

V

T

-

G

M

D

T

K

V

V

F

F

T

E

M
x
W

V

L

T

L

T

N

V

I

S

T

A

G

I

V

L

A

F

G

M
x
F

F

F

V

A

W

W

T

L

I

F

I

I

L

S

C

I

S

S

Y

H

L

I

A

R

Y

F

R

M

K

Q

Q

A

H

L

P

K

Q

Y

R

R

H

-

A

G

A

I

S

S

K

R

F

D

K

E

M

L

P

P

L

F

-

K

G

A

K

K

F

L

M

M

C

P

W

G

V

L

C

A

M

Y

A

Y

F

A

A

T

F

F

V

M

L

T

V

I

L

I

T

I

L

I

Q

Q

P113 (≠ T47) mutation to L: In CAN1-343; confers citrulline transport activity in GAP1-deleted cells.
P148 (≠ L81) mutation to L: In CAN1-337; confers citrulline transport activity in GAP1-deleted cells and leads to sensitivity to L-glutamic acid alpha-hydroxamate, alpha-aminoisobutyrate, 3-chloro-L-alanine, L-ethionine, L-allylglycine, and D-histidine, but not sensitivity to L-aspartic acid alpha-hydroxamate or p-fluoro-L-phenylalanine.
V149 (≠ E82) mutation to F: In CAN1-315; confers citrulline transport activity in GAP1-deleted cells.
S152 (= S85) mutation to F: In CAN1-342; confers citrulline transport activity in GAP1-deleted cells.
Y173 (= Y106) mutation to D: In CAN1-306; confers citrulline transport activity in GAP1-deleted cells.; mutation to H: In CAN1-327; confers citrulline transport activity in GAP1-deleted cells.
G308 (≠ A235) mutation to A: In CAN1-341; confers citrulline transport activity in GAP1-deleted cells.
P313 (= P240) mutation to S: In CAN1-329; confers citrulline transport activity in GAP1-deleted cells and leads to sensitivity to L-glutamic acid alpha-hydroxamate, alpha-aminoisobutyrate, 3-chloro-L-alanine, L-ethionine, L-allylglycine, and D-histidine, L-aspartic acid alpha-hydroxamate and p-fluoro-L-phenylalanine.
TS 354:355 (≠ -V 275) mutation Missing: In CAN1-318; confers citrulline transport activity in GAP1-deleted cells.
Y356 (≠ L276) mutation to H: In CAN1-340; confers citrulline transport activity in GAP1-deleted cells.; mutation to N: In CAN1-339; confers citrulline transport activity in GAP1-deleted cells.
W451 (≠ M373) mutation to C: In CAN1-328; confers citrulline transport activity in GAP1-deleted cells.; mutation to L: In CAN1-316; confers citrulline transport activity in GAP1-deleted cells.; mutation to S: In CAN1-335; confers citrulline transport activity in GAP1-deleted cells.
F461 (≠ M383) mutation to S: In CAN1-307; confers citrulline transport activity in GAP1-deleted cells.

P19145 General amino-acid permease GAP1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 3 papers)
31% identity, 86% coverage: 1:398/463 of query aligns to 71:476/602 of P19145

query
sites
P19145

M

L

V

S

D

E

Q

A

S

E

K
|
K

E

V

A

A

L

I

I

I

E

T

A

A

E

Q

Q

T

P

P

E

-

K

-

L

L

R

K

R

H

N

H

L

L

H

K

N

N

R

R

H

H

I

L

Q

Q

L

M

I

I

A

A

I

I

G

G

A

A

I

I

G

G

T

T

G

G

L

L

F

L

M

V

G

G

S

S

G

G

K

T

T

A

I

L

S

R

L

T

A

G

G

G

P

P

-

A

S

S

I

L

F

I

V

G

Y

W

M

G

I

S

I

T

G

G

F

T

M

M

L

I

F

Y

F

A

V

M

M

V

R

M

A

A

M

L

G

G

E

E

L

L

L

A

L

V

S

I

N

F

L

P

E

I

Y

S

K

G

S

G

F

F

S

T

D

T

F

Y

A

A

A

T

D

R

L

F

L

I

G

D

P

E

W

S

A

F

G

G

Y

Y

F

A

T

N

G

N

W

F

T

N

Y

Y

W

M

F

L

C

Q

W

W

V

L

V

V

T

V

G

L

I

P

A

L

D

E

V

I

V

V

A

S

I

A

S

S

A

I

Y

T

F

V

Q

N

L

F

W

W

-

G

-

T

F

D

P

P

G

K

F

Y

S

R

I

D

W

G

M

F

S

V

A

A

L

L

L

F

C

W

I

L

F

-

A

A

F

I

L

V

A

I

L

I

N

N

I

M

A

F

T

G

V

V

K

K

L

G

F

Y

G

G

E

E

M

A

E

E

F

F

W

V

F

F

A

S

I

F

I

I

K

K

I

V

V

I

A

T

I

V

V

V

A

G

L

F

I

I

T

L

G

G

I

I

V

I

L

L

V

-

S

-

M

-

H

N

Y

C

P

G

S

G

P

G

N

P

G

T

T

G

A

Y

S

I

L

G

S

G

N

K

I

Y

W

W

D

H

H

D

G

P

G

G

M

A

F

F

P

A

K

G

G

D

L

T

S

P

G

G

F

A

-

K

F

F

A

K

G

G

-

V

-

C

-

S

-

V

F

F

Q

V

I

T

A

A

V

A

F

F

A

S

F

F

V
x
A

G

G

I

S

E

E

L

L

V

V

G

G

T

L

A

A

A

S

E

E

T

S

K

V

D

E

P

P

H

R

K

K

V

S

L

V

P

P

R

K

A

A

I

A

N

K

A

Q

I

V

P

F

V

W

R

R

I

I

I

T

M

L

F

F

Y

Y

V

I

L

L

A

S

L

L

V

M

I

I

M

G

A

L

V

L

T

V

P

P

W

Y

N

N

Q

D

-

K

-

S

-

L

-

I

-

G

-

A

-

S

V

V

L

D

P

A

N

A

R

A

S

S

P

P

F

F

V

V

E

I

M

A

F

I

V

K

L

T

I

H

G

G

L

I

P

K

A

G

A

L

A

P

S

S

I

V

V

V

N

N

F

V

V

V

V

I

L

L

T

I

S

A

A

V

A

L

S

S

S

V

A

G

N

N

S

S

G

A

I

I

F

Y

S

A

T

C

S

S

R

R

M

T

L

M

F

V

G

A

L

L

A

A

E

E

L

Q

G

R

V

F

A

L

H

P

K

E

A

I

F

F

G

S

R

Y

L

V

S

D

A

R

R

K

A

G

V

R

P

P

T

L

T

V

G

G

L

I

F

A

F

V

S

T

C

S

L

A

C

F

L

G

L

L

V

-

G

-

V

I

A

A

L

F

I

V

Y

A

L

A

I

S

P

K

D

K

V

E

M

G

T

E

V

V

F

F

T

N

M

W

V

L

T

L

T

A

V

L

S

S

A

G

I

L

L

S

F

S

M

L

F

F

V

T

W

W

T

G

I

G

I

I

L

C

C

I

S

C

Y

H

L

I

A

R

Y

F

R

R

K

K

K76 (= K6) modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
A297 (≠ V225) mutation to V: Impairs basic amino-acids transport and regulation by these amino-acids.

P48813 High-affinity glutamine permease from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
27% identity, 86% coverage: 2:398/463 of query aligns to 128:535/663 of P48813

query
sites
P48813

V

V

D

D

Q

G

S

I

K
|
K

E

Q

A

S

L

P

I

Q

E

E

A

Q

E

E

Q

Q

P

K

-

Q

E

E

K

N

L

L

R

K

N

S

L

I

H

K

N

P

R

R

H

H

I

T

Q

V

L

M

I

M

A

S

I

L

G

G

G

T

A

G

I

I

G

G

T

T

G

G

L

L

F

L

M

V

G

G

S

N

G

S

K

K

T

V

I

L

S

N

L

N

A

A

G

G

P

P

S

G

-

G

I

L

I

I

F

I

V

G

Y

Y

M

A

I

I

I

M

G

G

F

S

M

C

L

V

F

Y

F

C

V

I

M

I

R

Q

A

A

M

C

G

G

E

E

L

L

-

A

-

V

L

I

L

Y

S

S

N

D

L

L

E

-

Y

I

K

G

S

G

F

F

S

N

D

T

F

Y

A

P

A

L

D

F

L

L

L

V

G

D

P

P

W

A

A

L

G

G

Y

F

F

S

T

V

G

A

W

W

T

L

Y

F

W

C

F

L

C

Q

W

W

V

L

V

C

T

V

G

C

I

P

A

L

D

E

V

L

V

V

A

T

I

A

S

S

A

M

Y

T

F

I

Q

K

L

Y

W

W

F

T

P

T

G

-

F

-

S

S

I

V

W

N

M

P

S

D

A

V

L

F

L

V

C

V

I

I

F

F

A

Y

F

V

L

L

-

I

-

V

A

V

L

I

N

N

I

V

A

F

T

G

V

A

K

K

L

G

F

Y

G

A

E

E

M

A

E

D

F

F

W

F

F

F

A

N

I

C

K

K

I

I

V

L

A

M

I

I

V

V

A

G

L

F

I

F

I

I

T

L

G

A

I

I

V

I

L

I

V

D

S

C

M

-

H

-

Y

-

P

-

S

G

P

G

N

A

G

G

T

T

T

D

A

G

S

Y

L

I

-

G

S

S

N

K

I

Y

W

W

D

R

H

D

G

P

G

G

M

A

F

F

P

R

-

G

-

D

-

T

-

P

-

I

K

Q

G

R

L

F

S

K

G

G

F

V

A

A

G

T

F

F

Q

V

I

T

A

A

V

A

F

F

A

A

F

F

V

G

G

M

I

S

E

E

L

Q

V

L

G

A

T

M

A

T

A

A

A

S

E

E

T

Q

K

S

D

N

P

P

H

R

K

K

V

A

L

I

P

P

R

S

A

A

I

A

N

K

A

K

I

M

P

I

V

Y

R

R

I

I

I

L

M

F

F

V

Y

F

V

L

L

A

A

S

L

L

L

T

V

L

I

V

M

G

A

F

V

L

T

V

P

P

W

Y

-

T

-

S

N

D

Q

Q

V

L

L

L

-

G

-

A

-

G

-

S

-

A

P

T

N

K

R

A

S

S

P

P

F

Y

V

V

E

I

M

A

F

V

V

S

L

S

I

H

G

G

L

V

P

R

A

V

A

P

S

H

I

F

V

I

N

N

F

A

V

V

V

I

L

L

T

L

S

S

A

V

A

L

S

S

S

V

A

A

N

N

S

G

G

A

I

F

F

Y

S

T

T

S

S

S

R

R

M

I

L

L

F

M

G

S

L

L

A

A

E

K

L

Q

G

G

V

N

A

A

H

P

K

K

A

C

F

F

G

D

R

Y

L

I

S

D

A

R

R

E

A

G

V

R

P

P

T

A

G

A

L

M

F

L

F

V

S

S

C

A

L

L

C

F

L

G

L

V

V

-

G

-

V

I

A

A

L

F

I

C

Y

A

L

S

I

S

P

K

D

K

V

E

M

E

T

D

V

V

F

F

T

T

M

W

V

L

T

L

T

A

V

I

S

S

A

G

I

L

L

S

F

Q

M

L

F

F

V

T

W

W

T

I

I

T

I

I

L

C

C

L

S

S

Y

H

L

I

A

R

Y

F

R

R

K

R

K132 (= K6) modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Q03770 SPS-sensor component SSY1; Amino-acid permease homolog SSY1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
23% identity, 88% coverage: 11:418/463 of query aligns to 270:756/852 of Q03770

query
sites
Q03770

E

R

A

A

E

T

Q

R

P

K

E

Y

K

H

L

I

R

Q

R

R

N

K

L

L

H

K

N

V

R

R

H

H

I

I

Q

Q

L

M

I

L

A

S

I

I

G

G

G

A

A

C

I

F

G

S

T

V

G

G

L

L

F

F

M

L

G

T

S

S

G

G

K

K

T

A

I

F

S

S

L

I

A

A

G

G

P

P

-

F

S

G

I

T

I

L

F

L

V

G

Y

F

M

G

I

L

I

T

G

G

F

S

M

I

L

I

F

L

F

A

V

T

M

M

R

L

A

S

M

F

G

T

E

E

L

L

L

S

L

T

S

L

N

I

L

P

E

V

Y

S

K

S

S

G

F

F

S

S

D

G

F

L

A

A

A

S

D

R

L

F

L

V

G

E

P

D

W

A

A

F

G

G

Y

F

F

A

T

L

G

G

W

W

T

T

Y

Y

W

W

F

I

C

S

W

C

V

M

V

L

T

A

G

L

I

P

A

A

D

Q

V

V

V

S

A

S

I

S

S
x
T

A

F

Y

Y

F

L

Q

S

L

Y

W

Y

F

N

-

N

-

V

-

N

-

I

-

S

P

K

G

G

F

V

S

T

I

A

W

G

M

F

S

I

A

T

L

L

L

F

C

S

I

A

F

F

A

S

F

-

L

I

A

V

L

V

N

N

I

L

A

L

T

D

V

V

K

S

L

I

F

M

G

G

E

E

M

I

E

V

F

Y

W

V

F

A

A

G

I

I

I

S

K

K

I

V

V

I

A

I

I

A

V

I

A

L

L

M

I

V

I

F

T

T

G

M

I

I

V

I

L

L

V

N

S

A

M

G

H

H

-

G

-

N

-

D

-

I

-

H

-

E

-

G

-

V

-

G

-

F

-

R

Y

Y

P

W

S

D

P

S

N

S

G

K

T

S

T

V

A

R

S

N

L

L

S

T

-

Y

-

G

-

L

-

Y

-

R

-

P

-

T

-

F

N

D

I

L

W

A

D

D

H

A

G

G

G

E

M

G

F

S

P

K

K

K

G

G

L

I

S

S

G

G

F

P

F

K

A

G

G

R

F

F

-

L

-

A

-

T

-

A

-

S

-

V

-

M

Q

L

I

I

A

S

V

T

F

F

A

A

F

F

V

S

G

G

I

V

E

E

L

M

V

T

G

F

T

L

A

A

A

S

A

G

E

E

T

A

K

I

D

N

P

P

H

R

K

K

V

T

L

I

P

P

R

S

A

A

I

T

N

K

A

R

I

T

P

F

V

S

R

I

I

V

I

L

M

I

F

S

Y

Y

V

V

L

F

A

L

L

I

-

F

-

S

-

V

-

G

L

I

V

N

I

I

M

Y

A

S

V

G

T

D

P

P

-

R

-

L

-

S

-

Y

-

F

-

P

-

G

-

I

-

S

-

E

-

K

-

R

-

Y

-

E

-

A

-

I

-

K

-

G

-

T

-

G

-

M

-

D

W

W

N

R

Q

L

V

R

L

T

P

N

N

C

R

R

-

G

-

I

-

D

-

Y

-

R

-

Q

-

I

-

S

-

V

-

G

-

T

-

G

-

Y

-

S

S

S

P

P

F

W

V

V

E

V

M

A

F

L

V

Q

L

N

I

F

G

G

L

L

P

C

A

T

A

F

A

A

S

S

I

A

V

F

N

N

F

A

V

I

V

L

L

I

T

F

S

F

A

T

A

A

S

T

S

A

A

G

N

I

S

S

G

S

I

L

F

F

S

S

T

C

S

S

R

R

M

T

L

L

F

Y

G

A

L

M

A

S

E

V

L

Q

G

R

V

K

A

A

H

P

K

P

A

V

F

F

G

E

R

I

L

C

S

S

A

K

R

R

A

G

V

V

P

P

T

Y

T

V

G

S

L

V

F

I

F

F

S

S

C

S

L

L

C

F

L

S

L

V

V

-

G

-

V

I

A

A

L

Y

I

I

Y

A

L

V

I

D

P

Q

D

T

V

A

M

I

T

E

V

N

F

F

T

D

M

V

V

L

T

A

T

N

V

V

S

S

A

S

I

A

L

S

F

T

M

S

F

I

V

I

W

W

T

M

I

G

I

L

L

N

C

L

S

S

Y

F

L

L

A

R

Y

F

-

Y

-

A

-

L

-

K

-

Q

R

R

K

K

Q

D

H

I

P

I

Q

S

R

R

H

N

A

D

A

S

S

S

-

Y

K

P

F

Y

K

K

M

S

P

P

L

F

G

Q

K

P

F

Y

M

L

C

A

T382 (≠ S122) mutation T->H,L: Constitutively active, up-regulates amino acid permease transcription in response to subthreshold concentrations of amino acids.; mutation to K: In SSY1-102; constitutively active, up-regulates amino acid permease transcription in the absence of amino-acids.; mutation to R: Constitutively active, up-regulates amino acid permease transcription in the absence of amino acids.

P30825 High affinity cationic amino acid transporter 1; CAT-1; CAT1; Ecotropic retroviral leukemia receptor homolog; Ecotropic retrovirus receptor homolog; Solute carrier family 7 member 1; System Y+ basic amino acid transporter from Homo sapiens (Human) (see paper)
21% identity, 72% coverage: 6:340/463 of query aligns to 15:381/629 of P30825

query
sites
P30825

K

R

E

R

A

K

L

V

I

V

E

D

A

C

E

S

Q

R

P

E

E

E

-

T

K

R

L

L

R

S

R

R

N

C

L

L

H

N

N

T

R

F

H

D

I

L

Q

V

L

A

I

L

A

G

I

V

G

G

G

S

A

T

I

L

G

G

T

A

G

G

L

V

F

Y

M

V

G

L

S

A

G

G

K

A

T

V

I

A

-

R

S

E

L

N

A

A

G

G

P

P

S

A

I

I

I

V

F

I

V

S

Y

F

M

L

I

I

I

A

G

A

F

L

M

A

L

S

F

V

F

L

V

A

M

G

R

L

A

C

M

Y

G

G

E

E

L

F

L

G

L

A

S

R

N

V

L

P

E

K

Y

T

K

G

S

S

F

A

S

Y

D

L

F

Y

A

S

A

Y

D

V

L

T

L

V

G

G

P

E

W

L

A

W

G

A

Y

F

F

I

T

T

G

G

W

W

T

N

Y

L

W

I

F

L

C

S

W

Y

V

I

T

-

G

-

I

-

A

-

D

-

V

-

A

G

I

T

S

S

A

S

Y

V

F

A

Q

R

L

A

W

W

F

S

P

A

G

T

F

F

S

D

I

E

W

L

M

I

S

G

A

R

L

P

L

I

C

G

I

E

F

F

A

S

F

R

L

T

A

H

L

M

N

T

I

L

A

N

T

A

V

P

K

G

L

V

F

L

G

A

E

E

M

N

E

P

F

D

W

I

F

F

A

A

I

V

I

I

-

I

-

L

-

I

-

L

-

T