SitesBLAST
Comparing WP_084935516.1 NCBI__GCF_002095475.1:WP_084935516.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P07117 Sodium/proline symporter; Proline carrier; Proline permease; Propionate transporter from Escherichia coli (strain K12) (see 4 papers)
83% identity, 100% coverage: 1:493/494 of query aligns to 1:493/502 of P07117
- R257 (= R257) mutation to C: Sodium-independent binding affinity for proline.
- C281 (= C281) mutation to S: Does not affect proline uptake activity. Confers resistance to N-ethylmaleimide. Na(+)-dependent proline binding activity is similar to wild-type carrier.
- C344 (= C344) mutation to S: Small decrease in proline uptake activity. Confers resistance to N-ethylmaleimide. Exhibits low Na(+)-dependent proline binding.
- C349 (= C349) mutation to S: Does not affect proline uptake activity. Sensitive to N-ethylmaleimide. Na(+)-dependent proline binding activity is similar to wild-type carrier.
- R376 (= R376) mutation R->E,Q: No change in activity.; mutation to K: Loss of activity.
Q92911 Sodium/iodide cotransporter; Na(+)/I(-) cotransporter; Natrium iodide transporter; Sodium-iodide symporter; Na(+)/I(-) symporter; Solute carrier family 5 member 5 from Homo sapiens (Human) (see 3 papers)
24% identity, 73% coverage: 30:391/494 of query aligns to 42:403/643 of Q92911
- A102 (vs. gap) natural variant: A -> P
- H226 (≠ K222) mutation H->A,D,E,K: Significant loss of iodide transport activity but no effect on its localization to the cell membrane.
- D237 (= D228) mutation to A: Loss of localization to the cell membrane, significant loss of iodide transport activity but no effect on homodimerization.
- Y242 (≠ L233) Required for homodimerization; mutation to A: Loss of localization to the cell membrane, significant loss of iodide transport activity but no effect on homodimerization. Reduced homodimerization; when associated with A-471. Loss of iodide transport activity; when associated with F-535.
- T243 (≠ N234) Required for homodimerization; mutation to A: Loss of localization to the cell membrane, significant loss of iodide transport activity but no effect on homodimerization. Reduced homodimerization; when associated with A-471.
Sites not aligning to the query:
- 471 Required for homodimerization; Q→A: No effect on localization to the cell membrane, iodide transport activity and homodimerization. Significant loss of homodimerization; when associated with A-242 or A243.
- 525 A→F: Loss of localization to the cell membrane, significant loss of iodide transport activity but no effect on homodimerization. Loss of iodide transport activity; when associated with A-242.
- 536 T → Q: requires 2 nucleotide substitutions
- 556 S → Q: requires 2 nucleotide substitutions
Q9NY91 Probable glucose sensor protein SLC5A4; Solute carrier family 5 member 4 from Homo sapiens (Human) (see paper)
22% identity, 67% coverage: 108:437/494 of query aligns to 126:492/659 of Q9NY91
- E457 (≠ S402) mutation to Q: Confers sugar transport activity not found in the wild-type protein. Increased sensitivity to inhibitor phlorizin.
3dh4A Crystal structure of sodium/sugar symporter with bound galactose from vibrio parahaemolyticus (see paper)
24% identity, 74% coverage: 72:435/494 of query aligns to 43:426/512 of 3dh4A
Sites not aligning to the query:
Q8N695 Sodium-coupled monocarboxylate transporter 1; Apical iodide transporter; Electrogenic sodium monocarboxylate cotransporter; Sodium iodide-related cotransporter; Solute carrier family 5 member 8 from Homo sapiens (Human) (see 3 papers)
23% identity, 87% coverage: 30:457/494 of query aligns to 40:467/610 of Q8N695
- V193 (≠ A191) to I: in dbSNP:rs1709189
- F251 (≠ L242) to V: in dbSNP:rs11834933
Sites not aligning to the query:
- 608 T→A: Loss of interaction with PDZK1.
- 608:610 PDZ-binding
- 610 L→A: Loss of interaction with PDZK1.
9bimA Cryo-em structure of human cht1 in the hc-3 bound outward-facing state
25% identity, 78% coverage: 8:390/494 of query aligns to 7:393/516 of 9bimA
Sites not aligning to the query:
8j74A Human high-affinity choline transporter cht1 in the hc-3-bound outward-facing open conformation, dimeric state (see paper)
25% identity, 78% coverage: 8:390/494 of query aligns to 6:394/515 of 8j74A
- binding Lauryl Maltose Neopentyl Glycol: F203 (= F204), S206 (≠ I207), H207 (≠ A208), I213 (≠ D214), G214 (≠ S215), F215 (≠ L216), H219 (≠ E220), C326 (≠ F322), P327 (≠ N323)
- binding (2S,2'S)-2,2'-biphenyl-4,4'-diylbis(2-hydroxy-4,4-dimethylmorpholin-4-ium): W60 (≠ D55), Y89 (≠ L82), W139 (≠ F137), L245 (= L242), W252 (≠ H253)
Sites not aligning to the query:
Q9ET37 Solute carrier family 5 member 4A; SGLT3-a from Mus musculus (Mouse) (see paper)
23% identity, 67% coverage: 108:437/494 of query aligns to 126:492/656 of Q9ET37
- E457 (≠ A406) mutation to Q: Confers sodium-dependent sugar transport activity not found in the wild type protein.
8zqpA High affinity choline transporter 1
25% identity, 78% coverage: 8:390/494 of query aligns to 5:393/470 of 8zqpA
Q9GZV3 High affinity choline transporter 1; hCHT1; Hemicholinium-3-sensitive choline transporter; CHT; Solute carrier family 5 member 7 from Homo sapiens (Human) (see 5 papers)
24% identity, 78% coverage: 8:390/494 of query aligns to 8:396/580 of Q9GZV3
- D48 (≠ S41) to G: in CMS20; decreased choline transmembrane transporter activity; no effect on localization at plasma membrane; dbSNP:rs886039768
- G65 (= G58) to E: in CMS20; loss of choline transmembrane transporter activity; no effect on localization at plasma membrane; dbSNP:rs886039765
- I89 (= I80) to V: 40% reduction in choline transmembrane transporter activity; found in 0.06 of Ashkenazi Jews; dbSNP:rs1013940; mutation to A: Decreased choline transmembrane transporter activity, only 20% of wild-type choline uptake activity.
- P105 (≠ T101) to S: in CMS20; decreased choline transmembrane transporter activity; no effect on localization at plasma membrane; dbSNP:rs886039766
- Y111 (≠ A107) to H: in CMS20; no effect on localization at plasma membrane
- Y175 (= Y174) to C: in CMS20; uncertain significance; dbSNP:rs1331713195
- I291 (≠ V288) to T: in CMS20; uncertain significance; dbSNP:rs375397889
- V344 (= V338) to L: in CMS20; uncertain significance
- R361 (≠ E355) to Q: in CMS20; decreased choline transmembrane transporter activity; no effect on localization at plasma membrane; dbSNP:rs147656110
Sites not aligning to the query:
- 418 F → V: in CMS20; uncertain significance
- 446 R → G: in CMS20; decreased choline transmembrane transporter activity; no effect on localization at plasma membrane
- 451 E→Q: Decreased choline transmembrane transporter activity, only 5% of wild-type choline uptake activity.
- 527:532 Dileucine-like motif
- 530 I→A: No change in protein internalization. No change in choline transmembrane transporter activity.
- 531 L→A: Loss of protein internalization to vesicular structures in neurons. Increased choline transmembrane transporter activity.
- 531:532 LV→AA: Decreased protein internalization; when associated with V-538. Increased choline transmembrane transporter activity; when associated with V-538.
- 532 V→A: Decreased protein internalization. Increased choline transmembrane transporter activity.
- 538 K→V: Decreased protein internalization; when associated with 531-L-V-532. Increased choline transmembrane transporter activity; when associated with 531-L-V-532.
P11170 Sodium/glucose cotransporter 1; Na(+)/glucose cotransporter 1; High affinity sodium-glucose cotransporter; Solute carrier family 5 member 1 from Oryctolagus cuniculus (Rabbit) (see 2 papers)
30% identity, 30% coverage: 288:437/494 of query aligns to 359:492/662 of P11170
- Q457 (≠ S402) mutation to W: Drasticly decreased affinity for glucose and phlorizin.
- T460 (≠ W405) mutation to W: Decreased affinity for glucose and phlorizin.
Sites not aligning to the query:
- 255 modified: Disulfide link with 608
- 608 modified: Disulfide link with 255
7sl9A Cryoem structure of smct1 (see paper)
22% identity, 84% coverage: 41:457/494 of query aligns to 30:446/497 of 7sl9A
7yniA Structure of human sglt1-map17 complex bound with substrate 4d4fdg in the occluded conformation (see paper)
26% identity, 35% coverage: 299:471/494 of query aligns to 328:490/566 of 7yniA