SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_085121995.1 NCBI__GCF_900177295.1:WP_085121995.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

2r00C Crystal structure of aspartate semialdehyde dehydrogenase ii complexed with asa from vibrio cholerae (see paper)
54% identity, 99% coverage: 3:337/337 of query aligns to 4:336/336 of 2r00C

query
sites
2r00C

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active site: C131 (= C131), Q158 (= Q158), R237 (= R236), H244 (= H243)
binding 2,2'-oxydiacetic acid: V15 (= V14), N95 (= N95), C131 (= C131), N315 (= N316)

P23247 Aspartate-semialdehyde dehydrogenase 2; ASA dehydrogenase 2; ASADH 2; Aspartate-beta-semialdehyde dehydrogenase 2; EC 1.2.1.11 from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) (see paper)
54% identity, 99% coverage: 3:337/337 of query aligns to 5:337/337 of P23247

query
sites
P23247

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C132 (= C131) active site, Acyl-thioester intermediate

Q04797 Aspartate-semialdehyde dehydrogenase; ASA dehydrogenase; ASADH; Aspartate-beta-semialdehyde dehydrogenase; EC 1.2.1.11 from Bacillus subtilis (strain 168) (see paper)
51% identity, 98% coverage: 2:332/337 of query aligns to 4:340/346 of Q04797

query
sites
Q04797

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S98 (= S97) modified: Phosphoserine
Y146 (≠ V147) modified: Phosphotyrosine

8jusA Crystal structure of aspartate semialdehyde dehydrogenase from porphyromonas gingivalis complexed with 2',5'adenosine diphosphate
48% identity, 98% coverage: 4:332/337 of query aligns to 2:329/335 of 8jusA

query
sites
8jusA

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W

I

V

E

E

F

T

E

E

D

R

E

P

L

I

D

A

E

P

N

E

E

E

A

A

R

R

E

A

I

A

L

F

R

A

D

K

A

A

P

P

G

G

V

V

S

L

V

L

F

C

D

D

H

E

R

P

Q

S

T

E

E

K

G

Q

Y

Y

V

P

T

M

P

P

A

L

E

F

C

G

A

T

G

E

E

Q

D

D

A

P

V

V

Y

I

V

V

S

G

R

R

I

I

R

R

N

Q

D

D

P

L

T

A

I

N

D

P

H

N

G

G

L

L

S

V

L

F

W

W

V

C

V

V

S

S

D

D

N

Q

L

I

R

R

K

K

G

G

A

A

A

A

L

L

N

N

A

A

V

V

Q

Q

I

I

A

A

E

E

A

Y

L

L

binding adenosine-2'-5'-diphosphate: G7 (= G9), S9 (≠ T11), G10 (= G12), G34 (≠ A36), S35 (= S37), R37 (= R39), S38 (= S40), S70 (= S72), A71 (≠ P73), T75 (≠ V77)

4r54A Complex crystal structure of sp-aspartate-semialdehyde-dehydrogenase with 3-carboxy-ethyl-phthalic acid (see paper)
46% identity, 100% coverage: 2:337/337 of query aligns to 1:345/357 of 4r54A

query
sites
4r54A

G

G

Y

Y

R

T

V

V

A

A

V

V

V

V

G
|
G

A

A

T
|
T

G
|
G

N
x
A

V
|
V

G

G

R

A

E

Q

M

M

L

I

S

K

T

M

L

L

A

E

E

E

R

S

Q

T

F

L

P

P

V

I

D

D

E

K

V

I

V

R

A

Y

L

L

A
|
A

S
|
S

E

A

R
|
R

S
|
S

A

A

G

G

Q

K

K

S

V

L

S

K

F

F

G

-

D

K

D

D

Q

Q

V

D

L

I

Q

T

V

I

Q

E

D

E

L
x
T

A

T

S

E

F

T

D

A

F

F

K

E

G

G

I

V

D

D

I

I

G

A

L

L

F

F

S
|
S

P
x
A

G
|
G

A
x
S

K

S

V
x
T

S

S

A

A

E

K

F

Y

A

A

P

P

R

Y

A

A

A

V

R

K

A

A

G

G

T

V

I

V

V

V

I

V

D

D

N
|
N

T
|
T

S
|
S

Q

Y

F

F

R
|
R

M

Q

D

N

P

P

D

D

V

V

P

P

L

L

V

V

V

V

P

P

E

E

V

V

N

N

R

A

E

H

A

A

I

L

A

D

G

A

Y

H

P

-

R

-

R

N

N

G

I

I

I

I

A

A

N

C

P

P

N
|
N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

A

A

L

L

K

E

P

P

L

V

H

R

D

Q

R

K

V

W

P

G

I

L

R

D

R

R

V

I

V

I

V

V

S

S

T

T

Y

Y

Q

Q

S

A

V

V

S

S

G

G

A

A

G
|
G

K

M

E

G

A

A

M

I

D

L

E

E

L

T

F

Q

N

R

Q

E

T

L

R

R

G

E

I

V

Y

-

V

L

N

N

D

D

P

G

I

V

K

K

-

P

-

C

-

D

-

L

-

H

T

A

E

E

I

I

F

L

P

P

K

S

Q

G

-

G

-

D

-

K

-

K

-

H

-

Y

-

P

I

I

A

A

F

F

N

N

V

A

I

L

P

P

Q

Q

I

I

D

D

V

V

F

F

M

T

E

D

D

N

G

D

S

Y

T

T

K

Y

E

E

E

E

W

M

K
|
K

M

M

A

T

V

K

E

E

T

T

K

K

K

K

I

I

L

M

-

E

D

D

P

D

R

S

I

I

K

A

V

V

H

S

A

A

T

T

C

C

V

V

R

R

V

I

P

P

T

V

F

L

I

S

G

A

H

H

A

S

E

E

A

S

V

V

N

Y

I

I

E

E

F

T

E

K

D

E

E

V

L

A

D

P

E

I

N

E

E

E

A

V

R

K

E

A

I

A

L

I

R

A

D

A

A

F

P

P

G

G

V

A

S

V

V

L

F

E

D

D

H

D

R

V

Q

A

T

H

E

Q

G

I

Y

Y

V

P

T

Q

P

A

A

I

E

N

C

A

A

V

G

G

E

S

D

R

A

D

V

T

Y

F

V

V

S

G

R

R

I

I

R

R

N

K

D

D

P

L

T

D

I

A

D

E

H

K

G

G

L

I

S

H

L

M

W

W

V

V

V

V

S

S

D

D

N

N

L

L

R

L

K

K

G
|
G

A

A

A

A

L

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

A

T

L

L

A

H

E

E

E

R

Y

G

L

L

binding 3-(2-carboxyethyl)benzene-1,2-dicarboxylic acid: G72 (= G74), S73 (≠ A75), T94 (= T96), S95 (= S97), R98 (= R100), K222 (= K215)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G9), T10 (= T11), G11 (= G12), A12 (≠ N13), V13 (= V14), A35 (= A36), S36 (= S37), R38 (= R39), S39 (= S40), T56 (≠ L58), S70 (= S72), A71 (≠ P73), G72 (= G74), T75 (≠ V77), N93 (= N95), T94 (= T96), N126 (= N130), C127 (= C131), G160 (= G164), G328 (= G320)

4r41A Complex crystal structure of 4-nitro-2-phosphono-benzoic acid with sp- aspartate-semialdehyde dehydrogenase and nicotinamide-dinucleotide (see paper)
46% identity, 100% coverage: 2:337/337 of query aligns to 1:345/357 of 4r41A

query
sites
4r41A

G

G

Y

Y

R

T

V

V

A

A

V

V

V

V

G
|
G

A

A

T
|
T

G
|
G

N
x
A

V

V

G

G

R

A

E

Q

M

M

L

I

S

K

T

M

L

L

A

E

E

E

R

S

Q

T

F

L

P

P

V

I

D

D

E

K

V

I

V

R

A

Y

L

L

A
|
A

S
|
S

E

A

R
|
R

S
|
S

A

A

G

G

Q

K

K

S

V

L

S

K

F

F

G

-

D

K

D

D

Q

Q

V

D

L

I

Q

T

V

I

Q

E

D

E

L
x
T

A

T

S

E

F

T

D

A

F

F

K

E

G

G

I

V

D

D

I

I

G

A

L

L

F

F

S
|
S

P
x
A

G
|
G

A
x
S

K

S

V

T

S

S

A

A

E

K

F

Y

A

A

P

P

R

Y

A

A

A

V

R

K

A

A

G

G

T

V

I

V

V

V

I

V

D

D

N
|
N

T
|
T

S
|
S

Q

Y

F

F

R
|
R

M

Q

D

N

P

P

D

D

V

V

P

P

L

L

V

V

V

V

P

P

E

E

V

V

N

N

R

A

E

H

A

A

I

L

A

D

G

A

Y

H

P

-

R

-

R

N

N

G

I

I

I

I

A

A

N

C

P

P

N

N

C

C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

A

A

L

L

K

E

P

P

L

V

H

R

D

Q

R

K

V

W

P

G

I

L

R

D

R

R

V

I

V

I

V

V

S

S

T

T

Y

Y

Q

Q

S

A

V

V

S

S

G

G

A

A

G
|
G

K
x
M

E
x
G

A

A

M

I

D

L

E

E

L

T

F

Q

N

R

Q

E

T

L

R

R

G

E

I

V

Y

-

V

L

N

N

D

D

P

G

I

V

K

K

-

P

-

C

-

D

-

L

-

H

T

A

E

E

I

I

F

L

P

P

K

S

Q

G

-

G

-

D

-

K

-

K

-

H

-

Y

-

P

I

I

A

A

F

F

N

N

V

A

I

L

P

P

Q

Q

I

I

D

D

V

V

F

F

M

T

E

D

D

N

G

D

S

Y

T

T

K

Y

E

E

E

E

W

M

K
|
K

M

M

A

T

V

K

E

E

T

T

K

K

K

K

I

I

L

M

-

E

D

D

P

D

R

S

I

I

K

A

V

V

H

S

A

A

T

T

C

C

V

V

R

R

V

I

P

P

T

V

F

L

I

S

G

A

H

H

A

S

E

E

A

S

V

V

N

Y

I

I

E

E

F

T

E

K

D

E

E

V

L

A

D

P

E

I

N

E

E

E

A

V

R

K

E

A

I

A

L

I

R

A

D

A

A

F

P

P

G

G

V

A

S

V

V

L

F

E

D

D

H

D

R

V

Q

A

T

H

E

Q

G

I

Y

Y

V

P

T

Q

P

A

A

I

E

N

C

A

A

V

G

G

E

S

D

R

A

D

V

T

Y

F

V

V

S

G

R

R

I

I

R

R

N

K

D

D

P

L

T

D

I

A

D

E

H

K

G

G

L

I

S

H

L

M

W

W

V

V

V

V

S

S

D

D

N

N

L

L

R

L

K

K

G

G

A

A

A

A

L

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

A

T

L

L

A

H

E

E

E

R

Y

G

L

L

binding 4-nitro-2-phosphonobenzoic acid: S70 (= S72), G72 (= G74), S73 (≠ A75), N93 (= N95), T94 (= T96), S95 (= S97), R98 (= R100), K222 (= K215)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G9), T10 (= T11), G11 (= G12), A12 (≠ N13), A35 (= A36), S36 (= S37), R38 (= R39), S39 (= S40), T56 (≠ L58), A71 (≠ P73), G160 (= G164), M161 (≠ K165), G162 (≠ E166)

4r3nA Crystal structure of the ternary complex of sp-asadh with NADP and 1, 2,3-benzenetricarboxylic acid (see paper)
46% identity, 100% coverage: 2:337/337 of query aligns to 1:345/357 of 4r3nA

query
sites
4r3nA

G

G

Y

Y

R

T

V

V

A

A

V

V

V

V

G
|
G

A

A

T
|
T

G
|
G

N
x
A

V
|
V

G

G

R

A

E

Q

M

M

L

I

S

K

T

M

L

L

A

E

E

E

R

S

Q

T

F

L

P

P

V

I

D

D

E

K

V

I

V

R

A

Y

L

L

A
|
A

S
|
S

E

A

R

R

S
|
S

A

A

G

G

Q

K

K

S

V

L

S

K

F

F

G

-

D

K

D

D

Q

Q

V

D

L

I

Q

T

V

I

Q

E

D

E

L
x
T

A

T

S

E

F

T

D

A

F

F

K

E

G

G

I

V

D

D

I

I

G

A

L

L

F

F

S
|
S

P
x
A

G
|
G

A
x
S

K

S

V

T

S

S

A

A

E

K

F

Y

A

A

P

P

R

Y

A

A

A

V

R

K

A

A

G

G

T

V

I

V

V

V

I

V

D

D

N
|
N

T
|
T

S
|
S

Q

Y

F

F

R
|
R

M

Q

D

N

P

P

D

D

V

V

P

P

L

L

V

V

V

V

P

P

E

E

V

V

N

N

R

A

E

H

A

A

I

L

A

D

G

A

Y

H

P

-

R

-

R

N

N

G

I

I

I

I

A

A

N

C

P

P

N
|
N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

A

A

L

L

K

E

P

P

L

V

H

R

D

Q

R

K

V

W

P

G

I

L

R

D

R

R

V

I

V

I

V

V

S

S

T

T

Y

Y

Q
|
Q

S

A

V

V

S

S

G

G

A

A

G
|
G

K
x
M

E

G

A

A

M

I

D

L

E

E

L

T

F

Q

N

R

Q

E

T

L

R

R

G

E

I

V

Y

-

V

L

N

N

D

D

P

G

I

V

K

K

-

P

-

C

-

D

-

L

-

H

T

A

E

E

I

I

F

L

P

P

K

S

Q

G

-

G

-

D

-

K

-

K

-

H

-

Y

-

P

I

I

A

A

F

F

N

N

V

A

I

L

P

P

Q

Q

I

I

D

D

V

V

F

F

M

T

E

D

D

N

G

D

S

Y

T

T

K

Y

E

E

E

E

W

M

K
|
K

M

M

A

T

V

K

E

E

T

T

K

K

K

K

I

I

L

M

-

E

D

D

P

D

R

S

I

I

K

A

V

V

H

S

A

A

T

T

C

C

V

V

R
|
R

V

I

P

P

T

V

F

L

I

S

G

A

H
|
H

A

S

E

E

A

S

V

V

N

Y

I

I

E

E

F

T

E

K

D

E

E

V

L

A

D

P

E

I

N

E

E

E

A

V

R

K

E

A

I

A

L

I

R

A

D

A

A

F

P

P

G

G

V

A

S

V

V

L

F

E

D

D

H

D

R

V

Q

A

T

H

E

Q

G

I

Y

Y

V

P

T

Q

P

A

A

I

E

N

C

A

A

V

G

G

E

S

D

R

A

D

V

T

Y

F

V

V

S

G

R

R

I

I

R

R

N

K

D

D

P

L

T

D

I

A

D

E

H

K

G

G

L

I

S

H

L

M

W

W

V

V

V

V

S

S

D

D

N

N

L

L

R

L

K

K

G
|
G

A

A

A

A

L

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

A

T

L

L

A

H

E

E

E

R

Y

G

L

L

active site: C127 (= C131), Q154 (= Q158), R244 (= R236), H251 (= H243)
binding benzene-1,2,3-tricarboxylic acid: S73 (≠ A75), T94 (= T96), S95 (= S97), R98 (= R100), N126 (= N130), K222 (= K215)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G9), T10 (= T11), G11 (= G12), A12 (≠ N13), V13 (= V14), A35 (= A36), S36 (= S37), S39 (= S40), T56 (≠ L58), S70 (= S72), A71 (≠ P73), G72 (= G74), N93 (= N95), T94 (= T96), N126 (= N130), C127 (= C131), G160 (= G164), M161 (≠ K165), G328 (= G320)

3q1lA Crystals structure of aspartate beta-semialdehyde dehydrogenase from streptococcus pneumoniae with cysteamine bound covalently to cys 128 (see paper)
46% identity, 100% coverage: 2:337/337 of query aligns to 1:345/357 of 3q1lA

query
sites
3q1lA

G

G

Y

Y

R

T

V

V

A

A

V

V

V

V

G

G

A

A

T

T

G

G

N

A

V

V

G

G

R

A

E

Q

M

M

L

I

S

K

T

M

L

L

A

E

E

E

R

S

Q

T

F

L

P

P

V

I

D

D

E

K

V

I

V

R

A

Y

L

L

A

A

S

S

E

A

R

R

S

S

A

A

G

G

Q

K

K

S

V

L

S

K

F

F

G

-

D

K

D

D

Q

Q

V

D

L

I

Q

T

V

I

Q

E

D

E

L

T

A

T

S

E

F

T

D

A

F

F

K

E

G

G

I

V

D

D

I

I

G

A

L

L

F

F

S

S

P

A

G

G

A

S

K

S

V

T

S

S

A

A

E

K

F

Y

A

A

P

P

R

Y

A

A

A

V

R

K

A

A

G

G

T

V

I

V

V

V

I

V

D

D

N

N

T

T

S

S

Q

Y

F

F

R

R

M

Q

D

N

P

P

D

D

V

V

P

P

L

L

V

V

V

V

P

P

E

E

V

V

N

N

R

A

E

H

A

A

I

L

A

D

G

A

Y

H

P

-

R

-

R

N

N

G

I

I

I

I

A

A

N

C

P

P

N
|
N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

A

A

L

L

K

E

P

P

L

V

H

R

D

Q

R

K

V

W

P

G

I

L

R

D

R

R

V

I

V

I

V

V

S

S

T

T

Y

Y

Q
|
Q

S

A

V

V

S

S

G
|
G

A

A

G

G

K

M

E

G

A

A

M

I

D

L

E

E

L

T

F

Q

N

R

Q

E

T

L

R

R

G

E

I

V

Y

-

V

L

N

N

D

D

P

G

I

V

K

K

-

P

-

C

-

D

-

L

-

H

T

A

E

E

I

I

F

L

P

P

K

S

Q

G

-

G

-

D

-

K

-

K

-

H

-

Y

-

P

I

I

A

A

F

F

N

N

V

A

I

L

P

P

Q

Q

I

I

D

D

V

V

F

F

M

T

E

D

D

N

G

D

S

Y

T

T

K

Y

E
|
E

E

E

W

M

K

K

M

M

A

T

V

K

E

E

T

T

K

K

K

K

I

I

L

M

-

E

D

D

P

D

R

S

I

I

K

A

V

V

H

S

A

A

T

T

C

C

V

V

R
|
R

V

I

P

P

T

V

F

L

I

S

G

A

H
|
H

A

S

E

E

A

S

V

V

N

Y

I

I

E

E

F

T

E

K

D

E

E

V

L

A

D

P

E

I

N

E

E

E

A

V

R

K

E

A

I

A

L

I

R

A

D

A

A

F

P

P

G

G

V

A

S

V

V

L

F

E

D

D

H

D

R

V

Q

A

T

H

E

Q

G

I

Y

Y

V

P

T

Q

P

A

A

I

E

N

C

A

A

V

G

G

E

S

D

R

A

D

V

T

Y

F

V

V

S

G

R

R

I

I

R

R

N

K

D

D

P

L

T

D

I

A

D

E

H

K

G

G

L

I

S

H

L

M

W

W

V

V

V

V

S

S

D

D

N

N

L

L

R

L

K

K

G

G

A

A

A

A

L

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

A

T

L

L

A

H

E

E

E

R

Y

G

L

L

active site: C127 (= C131), Q154 (= Q158), R244 (= R236), H251 (= H243)
binding 2-amino-ethanethiol: N126 (= N130), C127 (= C131), G158 (= G162), E219 (= E212)

3pwsA Crystal structure of aspartate beta-semialdehide dehydrogenase from streptococcus pneumoniae with 2',5'-adenosine diphosphate and d-2- aminoadipate (see paper)
46% identity, 100% coverage: 2:337/337 of query aligns to 1:345/357 of 3pwsA

query
sites
3pwsA

G

G

Y

Y

R

T

V

V

A

A

V

V

V

V

G
|
G

A

A

T
|
T

G
|
G

N
x
A

V

V

G

G

R

A

E

Q

M

M

L

I

S

K

T

M

L

L

A

E

E

E

R

S

Q

T

F

L

P

P

V

I

D

D

E

K

V

I

V

R

A

Y

L

L

A
|
A

S
|
S

E

A

R
|
R

S
|
S

A

A

G

G

Q

K

K

S

V

L

S

K

F

F

G

-

D

K

D

D

Q

Q

V

D

L

I

Q

T

V

I

Q

E

D

E

L
x
T

A

T

S

E

F

T

D

A

F

F

K

E

G

G

I

V

D

D

I

I

G

A

L

L

F

F

S

S

P
x
A

G

G

A

S

K

S

V
x
T

S

S

A

A

E

K

F

Y

A

A

P

P

R

Y

A

A

A

V

R

K

A

A

G

G

T

V

I

V

V

V

I

V

D

D

N

N

T

T

S

S

Q

Y

F

F

R
|
R

M

Q

D

N

P

P

D

D

V

V

P

P

L

L

V

V

V

V

P

P

E

E

V

V

N

N

R

A

E

H

A

A

I

L

A

D

G

A

Y

H

P

-

R

-

R

N

N

G

I

I

I

I

A

A

N

C

P

P

N
|
N

C

C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

A

A

L

L

K

E

P

P

L

V

H

R

D

Q

R

K

V

W

P

G

I

L

R

D

R

R

V

I

V

I

V

V

S

S

T

T

Y

Y

Q

Q

S

A

V

V

S

S

G
|
G

A

A

G
|
G

K
x
M

E

G

A

A

M

I

D

L

E

E

L

T

F

Q

N

R

Q

E

T

L

R

R

G

E

I

V

Y

-

V

L

N

N

D

D

P

G

I

V

K

K

-

P

-

C

-

D

-

L

-

H

T

A

E

E

I

I

F

L

P

P

K

S

Q

G

-

G

-

D

-

K

-

K

-

H

-

Y

-

P

I

I

A

A

F

F

N

N

V

A

I

L

P

P

Q

Q

I
|
I

D

D

V

V

F

F

M

T

E

D

D

N

G

D

S

Y

T

T

K

Y

E
|
E

E

E

W

M

K
|
K

M

M

A

T

V

K

E

E

T

T

K

K

K

K

I

I

L

M

-

E

D

D

P

D

R

S

I

I

K

A

V

V

H

S

A

A

T

T

C

C

V

V

R
|
R

V

I

P

P

T

V

F

L

I

S

G

A

H

H

A

S

E

E

A

S

V

V

N

Y

I

I

E

E

F

T

E

K

D

E

E

V

L

A

D

P

E

I

N

E

E

E

A

V

R

K

E

A

I

A

L

I

R

A

D

A

A

F

P

P

G

G

V

A

S

V

V

L

F

E

D

D

H

D

R

V

Q

A

T

H

E

Q

G

I

Y

Y

V

P

T

Q

P

A

A

I

E

N

C

A

A

V

G

G

E

S

D

R

A

D

V

T

Y

F

V

V

S

G

R

R

I

I

R

R

N

K

D

D

P

L

T

D

I

A

D

E

H

K

G

G

L

I

S

H

L

M

W

W

V

V

V

V

S

S

D

D

N

N

L

L

R

L

K

K

G

G

A

A

A

A

L

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

A

T

L

L

A

H

E

E

E

R

Y

G

L

L

binding (2R)-2-aminohexanedioic acid: R98 (= R100), N126 (= N130), G158 (= G162), I208 (= I201), E219 (= E212), K222 (= K215), R244 (= R236)
binding adenosine-2'-5'-diphosphate: G8 (= G9), T10 (= T11), G11 (= G12), A12 (≠ N13), A35 (= A36), S36 (= S37), R38 (= R39), S39 (= S40), T56 (≠ L58), A71 (≠ P73), T75 (≠ V77), G160 (= G164), M161 (≠ K165)

3pwkA Crystal structure of aspartate beta-semialdehide dehydrogenase from streptococcus pneumoniae with 2',5'-adenosine diphosphate and d-2- aminoadipate (see paper)
46% identity, 100% coverage: 2:337/337 of query aligns to 1:345/357 of 3pwkA

query
sites
3pwkA

G

G

Y

Y

R

T

V

V

A

A

V

V

V

V

G
|
G

A

A

T
|
T

G
|
G

N
x
A

V

V

G

G

R

A

E

Q

M

M

L

I

S

K

T

M

L

L

A

E

E

E

R

S

Q

T

F

L

P

P

V

I

D

D

E

K

V

I

V

R

A

Y

L

L

A
|
A

S
|
S

E

A

R
|
R

S
|
S

A

A

G

G

Q

K

K

S

V

L

S

K

F

F

G

-

D

K

D

D

Q

Q

V

D

L

I

Q

T

V

I

Q

E

D

E

L
x
T

A

T

S

E

F

T

D

A

F

F

K

E

G

G

I

V

D

D

I

I

G

A

L

L

F

F

S

S

P
x
A

G

G

A

S

K

S

V
x
T

S

S

A

A

E

K

F

Y

A

A

P

P

R

Y

A

A

A

V

R

K

A

A

G

G

T

V

I

V

V

V

I

V

D

D

N

N

T

T

S

S

Q

Y

F

F

R
|
R

M

Q

D

N

P

P

D

D

V

V

P

P

L

L

V

V

V

V

P

P

E

E

V

V

N

N

R

A

E

H

A

A

I

L

A

D

G

A

Y

H

P

-

R

-

R

N

N

G

I

I

I

I

A

A

N

C

P

P

N
|
N

C

C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

A

A

L

L

K

E

P

P

L

V

H

R

D

Q

R

K

V

W

P

G

I

L

R

D

R

R

V

I

V

I

V

V

S

S

T

T

Y

Y

Q

Q

S

A

V

V

S

S

G
|
G

A
|
A

G
|
G

K

M

E

G

A

A

M

I

D

L

E

E

L

T

F

Q

N

R

Q

E

T

L

R

R

G

E

I

V

Y

-

V

L

N

N

D

D

P

G

I

V

K

K

-

P

-

C

-

D

-

L

-

H

T

A

E

E

I

I

F

L

P

P

K

S

Q

G

-

G

-

D

-

K

-

K

-

H

-

Y

-

P

I

I

A

A

F

F

N

N

V

A

I

L

P

P

Q

Q

I

I

D

D

V

V

F

F

M

T

E

D

D

N

G

D

S

Y

T

T

K

Y

E
|
E

E

E

W

M

K
|
K

M

M

A

T

V

K

E

E

T

T

K

K

K

K

I

I

L

M

-

E

D

D

P

D

R

S

I

I

K

A

V

V

H

S

A

A

T

T

C

C

V

V

R
|
R

V

I

P

P

T

V

F

L

I

S

G

A

H

H

A

S

E

E

A

S

V

V

N

Y

I

I

E

E

F

T

E

K

D

E

E

V

L

A

D

P

E

I

N

E

E

E

A

V

R

K

E

A

I

A

L

I

R

A

D

A

A

F

P

P

G

G

V

A

S

V

V

L

F

E

D

D

H

D

R

V

Q

A

T

H

E

Q

G

I

Y

Y

V

P

T

Q

P

A

A

I

E

N

C

A

A

V

G

G

E

S

D

R

A

D

V

T

Y

F

V

V

S

G

R

R

I

I

R

R

N

K

D

D

P

L

T

D

I

A

D

E

H

K

G

G

L

I

S

H

L

M

W

W

V

V

V

V

S

S

D

D

N

N

L

L

R

L

K

K

G

G

A

A

A

A

L

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

A

T

L

L

A

H

E

E

E

R

Y

G

L

L

binding 5'-o-monophosphoryladenylyl(2'->5')adenylyl(2'->5')adenosine: G8 (= G9), T10 (= T11), G11 (= G12), A12 (≠ N13), A35 (= A36), S36 (= S37), R38 (= R39), S39 (= S40), T56 (≠ L58), A71 (≠ P73), T75 (≠ V77), G160 (= G164)
binding trans-cyclohexane-1,4-dicarboxylic acid: R98 (= R100), N126 (= N130), G158 (= G162), A159 (= A163), E219 (= E212), K222 (= K215), R244 (= R236)

2gz3A Structure of aspartate semialdehyde dehydrogenase (asadh) from streptococcus pneumoniae complexed with NADP and aspartate- semialdehyde (see paper)
46% identity, 100% coverage: 2:337/337 of query aligns to 1:345/357 of 2gz3A

query
sites
2gz3A

G

G

Y

Y

R

T

V

V

A

A

V

V

V

V

G
|
G

A

A

T
|
T

G
|
G

N
x
A

V
|
V

G

G

R

A

E

Q

M

M

L

I

S

K

T

M

L

L

A

E

E

E

R

S

Q

T

F

L

P

P

V

I

D

D

E

K

V

I

V

R

A

Y

L

L

A
|
A

S
|
S

E

A

R
|
R

S
|
S

A

A

G

G

Q

K

K

S

V

L

S

K

F

F

G

-

D

K

D

D

Q

Q

V

D

L

I

Q

T

V

I

Q

E

D

E

L
x
T

A

T

S

E

F

T

D

A

F

F

K

E

G

G

I

V

D

D

I

I

G

A

L

L

F

F

S
|
S

P
x
A

G
|
G

A

S

K

S

V
x
T

S

S

A

A

E

K

F

Y

A

A

P

P

R

Y

A

A

A

V

R

K

A

A

G

G

T

V

I

V

V

V

I

V

D

D

N
|
N

T

T

S

S

Q

Y

F

F

R

R

M

Q

D

N

P

P

D

D

V

V

P

P

L

L

V

V

V

V

P

P

E

E

V

V

N

N

R

A

E

H

A

A

I

L

A

D

G

A

Y

H

P

-

R

-

R

N

N

G

I

I

I

I

A

A

N

C

P

P

N

N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

A

A

L

L

K

E

P

P

L

V

H

R

D

Q

R

K

V

W

P

G

I

L

R

D

R

R

V

I

V

I

V

V

S

S

T

T

Y

Y

Q
|
Q

S

A

V

V

S

S

G
|
G

A

A

G
|
G

K
x
M

E

G

A

A

M

I

D

L

E

E

L

T

F

Q

N

R

Q

E

T

L

R

R

G

E

I

V

Y

-

V

L

N

N

D

D

P

G

I

V

K

K

-

P

-

C

-

D

-

L

-

H

T

A

E

E

I

I

F

L

P

P

K

S

Q

G

-

G

-

D

-

K

-

K

-

H

-

Y

-

P

I

I

A

A

F

F

N

N

V

A

I

L

P

P

Q

Q

I

I

D

D

V

V

F

F

M

T

E

D

D

N

G

D

S

Y

T

T

K

Y

E
|
E

E

E

W

M

K

K

M

M

A

T

V

K

E

E

T

T

K

K

K

K

I

I

L

M

-

E

D

D

P

D

R

S

I

I

K

A

V

V

H

S

A

A

T

T

C

C

V

V

R
|
R

V

I

P

P

T

V

F

L

I

S

G

A

H
|
H

A

S

E

E

A

S

V

V

N

Y

I

I

E

E

F

T

E

K

D

E

E

V

L

A

D

P

E

I

N

E

E

E

A

V

R

K

E

A

I

A

L

I

R

A

D

A

A

F

P

P

G

G

V

A

S

V

V

L

F

E

D

D

H

D

R

V

Q

A

T

H

E

Q

G

I

Y

Y

V

P

T

Q

P

A

A

I

E

N

C

A

A

V

G

G

E

S

D

R

A

D

V

T

Y

F

V

V

S

G

R

R

I

I

R

R

N

K

D

D

P

L

T

D

I

A

D

E

H

K

G

G

L

I

S

H

L

M

W

W

V

V

V

V

S

S

D

D

N
|
N

L

L

R

L

K

K

G

G

A
|
A

A

A

L

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

A

T

L

L

A

H

E

E

E

R

Y

G

L

L

active site: C127 (= C131), Q154 (= Q158), R244 (= R236), H251 (= H243)
binding (2r)-2-amino-4-oxobutanoic acid: C127 (= C131), Q154 (= Q158), G158 (= G162), E219 (= E212), R244 (= R236), H251 (= H243)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G9), T10 (= T11), G11 (= G12), A12 (≠ N13), V13 (= V14), A35 (= A36), S36 (= S37), R38 (= R39), S39 (= S40), T56 (≠ L58), S70 (= S72), A71 (≠ P73), G72 (= G74), T75 (≠ V77), N93 (= N95), G158 (= G162), G160 (= G164), M161 (≠ K165), N324 (= N316), A329 (= A321)

2gz2A Structure of aspartate semialdehyde dehydrogenase (asadh) from streptococcus pneumoniae complexed with 2',5'-adp (see paper)
46% identity, 100% coverage: 2:337/337 of query aligns to 1:345/357 of 2gz2A

query
sites
2gz2A

G

G

Y

Y

R

T

V

V

A

A

V

V

V

V

G
|
G

A

A

T
|
T

G
|
G

N

A

V

V

G

G

R

A

E

Q

M

M

L

I

S

K

T

M

L

L

A

E

E

E

R

S

Q

T

F

L

P

P

V

I

D

D

E

K

V

I

V

R

A

Y

L

L

A
|
A

S
|
S

E

A

R
|
R

S
|
S

A

A

G

G

Q

K

K

S

V

L

S

K

F

F

G

-

D

K

D

D

Q

Q

V

D

L

I

Q

T

V

I

Q

E

D

E

L
x
T

A

T

S

E

F

T

D

A

F

F

K

E

G

G

I

V

D

D

I

I

G

A

L

L

F

F

S

S

P
x
A

G

G

A

S

K

S

V
x
T

S

S

A

A

E

K

F

Y

A

A

P

P

R

Y

A

A

A

V

R

K

A

A

G

G

T

V

I

V

V

V

I

V

D

D

N

N

T

T

S

S

Q

Y

F

F

R

R

M

Q

D

N

P

P

D

D

V

V

P

P

L

L

V

V

V

V

P

P

E

E

V

V

N

N

R

A

E

H

A

A

I

L

A

D

G

A

Y

H

P

-

R

-

R

N

N

G

I

I

I

I

A

A

N

C

P

P

N

N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

A

A

L

L

K

E

P

P

L

V

H

R

D

Q

R

K

V

W

P

G

I

L

R

D

R

R

V

I

V

I

V

V

S

S

T

T

Y

Y

Q
|
Q

S

A

V

V

S

S

G

G

A

A

G

G

K

M

E

G

A

A

M

I

D

L

E

E

L

T

F

Q

N

R

Q

E

T

L

R

R

G

E

I

V

Y

-

V

L

N

N

D

D

P

G

I

V

K

K

-

P

-

C

-

D

-

L

-

H

T

A

E

E

I

I

F

L

P

P

K

S

Q

G

-

G

-

D

-

K

-

K

-

H

-

Y

-

P

I

I

A

A

F

F

N

N

V

A

I

L

P

P

Q

Q

I

I

D

D

V

V

F

F

M

T

E

D

D

N

G

D

S

Y

T

T

K

Y

E

E

E

E

W

M

K

K

M

M

A

T

V

K

E

E

T

T

K

K

K

K

I

I

L

M

-

E

D

D

P

D

R

S

I

I

K

A

V

V

H

S

A

A

T

T

C

C

V

V

R
|
R

V

I

P

P

T

V

F

L

I

S

G

A

H
|
H

A

S

E

E

A

S

V

V

N

Y

I

I

E

E

F

T

E

K

D

E

E

V

L

A

D

P

E

I

N

E

E

E

A

V

R

K

E

A

I

A

L

I

R

A

D

A

A

F

P

P

G

G

V

A

S

V

V

L

F

E

D

D

H

D

R

V

Q

A

T

H

E

Q

G

I

Y

Y

V

P

T

Q

P

A

A

I

E

N

C

A

A

V

G

G

E

S

D

R

A

D

V

T

Y

F

V

V

S

G

R

R

I

I

R

R

N

K

D

D

P

L

T

D

I

A

D

E

H

K

G

G

L

I

S

H

L

M

W

W

V

V

V

V

S

S

D

D

N

N

L

L

R

L

K

K

G

G

A

A

A

A

L

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

A

T

L

L

A

H

E

E

E

R

Y

G

L

L

active site: C127 (= C131), Q154 (= Q158), R244 (= R236), H251 (= H243)
binding adenosine-2'-5'-diphosphate: G8 (= G9), T10 (= T11), G11 (= G12), A35 (= A36), S36 (= S37), R38 (= R39), S39 (= S40), T56 (≠ L58), A71 (≠ P73), T75 (≠ V77)

2gz1A Structure of aspartate semialdehyde dehydrogenase (asadh) from streptococcus pneumoniae complexed with NADP (see paper)
46% identity, 100% coverage: 2:337/337 of query aligns to 1:345/357 of 2gz1A

query
sites
2gz1A

G

G

Y

Y

R

T

V

V

A

A

V

V

V

V

G
|
G

A

A

T
|
T

G
|
G

N
x
A

V
|
V

G

G

R

A

E

Q

M

M

L

I

S

K

T

M

L

L

A

E

E

E

R

S

Q

T

F

L

P

P

V

I

D

D

E

K

V

I

V

R

A

Y

L

L

A
|
A

S
|
S

E

A

R
|
R

S
|
S

A

A

G

G

Q

K

K

S

V

L

S

K

F

F

G

-

D

K

D

D

Q

Q

V

D

L

I

Q

T

V

I

Q

E

D

E

L
x
T

A

T

S

E

F

T

D

A

F

F

K

E

G

G

I

V

D

D

I

I

G

A

L

L

F

F

S
|
S

P
x
A

G
|
G

A

S

K

S

V
x
T

S

S

A

A

E

K

F

Y

A

A

P

P

R

Y

A

A

A

V

R

K

A

A

G

G

T

V

I

V

V

V

I

V

D

D

N
|
N

T

T

S

S

Q

Y

F

F

R

R

M

Q

D

N

P

P

D

D

V

V

P

P

L

L

V

V

V

V

P

P

E

E

V

V

N

N

R

A

E

H

A

A

I

L

A

D

G

A

Y

H

P

-

R

-

R

N

N

G

I

I

I

I

A

A

N

C

P

P

N

N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

A

A

L

L

K

E

P

P

L

V

H

R

D

Q

R

K

V

W

P

G

I

L

R

D

R

R

V

I

V

I

V

V

S

S

T

T

Y

Y

Q
|
Q

S

A

V

V

S
|
S

G
|
G

A

A

G
|
G

K
x
M

E

G

A

A

M

I

D

L

E

E

L

T

F

Q

N

R

Q

E

T

L

R

R

G

E

I

V

Y

-

V

L

N

N

D

D

P

G

I

V

K

K

-

P

-

C

-

D

-

L

-

H

T

A

E

E

I

I

F

L

P

P

K

S

Q

G

-

G

-

D

-

K

-

K

-

H

-

Y

-

P

I

I

A

A

F

F

N

N

V

A

I

L

P

P

Q

Q

I

I

D

D

V

V

F

F

M

T

E

D

D

N

G

D

S

Y

T

T

K

Y

E

E

E

E

W

M

K

K

M

M

A

T

V

K

E

E

T

T

K

K

K

K

I

I

L

M

-

E

D

D

P

D

R

S

I

I

K

A

V

V

H

S

A

A

T

T

C

C

V

V

R
|
R

V

I

P

P

T

V

F

L

I