SitesBLAST
Comparing WP_085123321.1 NCBI__GCF_900177295.1:WP_085123321.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3q0jC Crystal structure of the mycobacterium tuberculosis crotonase in complex with the inhibitor acetoacetylcoa
45% identity, 97% coverage: 6:258/260 of query aligns to 4:255/255 of 3q0jC
- active site: A65 (= A68), M70 (≠ F73), T80 (≠ R83), F84 (≠ E87), G108 (= G111), E111 (= E114), P130 (= P133), E131 (= E134), V136 (≠ I139), P138 (= P141), G139 (= G142), L224 (≠ A227), F234 (= F237)
- binding acetoacetyl-coenzyme a: Q23 (≠ E26), A24 (≠ V27), L25 (≠ R28), A27 (= A30), A63 (= A66), G64 (= G67), A65 (= A68), D66 (= D69), I67 (≠ L70), K68 (= K71), M70 (≠ F73), F84 (≠ E87), G107 (= G110), G108 (= G111), E111 (= E114), P130 (= P133), E131 (= E134), P138 (= P141), G139 (= G142), M140 (≠ A143)
3q0gC Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
45% identity, 97% coverage: 6:258/260 of query aligns to 4:255/255 of 3q0gC
- active site: A65 (= A68), M70 (≠ F73), T80 (≠ R83), F84 (≠ E87), G108 (= G111), E111 (= E114), P130 (= P133), E131 (= E134), V136 (≠ I139), P138 (= P141), G139 (= G142), L224 (≠ A227), F234 (= F237)
- binding coenzyme a: L25 (≠ R28), A63 (= A66), I67 (≠ L70), K68 (= K71), Y104 (≠ F107), P130 (= P133), E131 (= E134), L134 (≠ V137)
3q0gD Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
45% identity, 97% coverage: 6:258/260 of query aligns to 3:250/250 of 3q0gD
- active site: A64 (= A68), M69 (≠ F73), T75 (≠ R83), F79 (≠ E87), G103 (= G111), E106 (= E114), P125 (= P133), E126 (= E134), V131 (≠ I139), P133 (= P141), G134 (= G142), L219 (≠ A227), F229 (= F237)
- binding Butyryl Coenzyme A: F225 (≠ L233), F241 (= F249)
3h81A Crystal structure of enoyl-coa hydratase from mycobacterium tuberculosis (see paper)
45% identity, 97% coverage: 6:258/260 of query aligns to 3:254/256 of 3h81A
- active site: A64 (= A68), M69 (≠ F73), T79 (≠ R83), F83 (≠ E87), G107 (= G111), E110 (= E114), P129 (= P133), E130 (= E134), V135 (≠ I139), P137 (= P141), G138 (= G142), L223 (≠ A227), F233 (= F237)
- binding calcium ion: F233 (= F237), Q238 (≠ K242)
1dubA 2-enoyl-coa hydratase, data collected at 100 k, ph 6.5 (see paper)
44% identity, 97% coverage: 6:257/260 of query aligns to 4:257/260 of 1dubA
- active site: A68 (= A68), M73 (≠ F73), S83 (≠ R83), L87 (≠ E87), G111 (= G111), E114 (= E114), P133 (= P133), E134 (= E134), T139 (≠ I139), P141 (= P141), G142 (= G142), K227 (≠ A227), F237 (= F237)
- binding acetoacetyl-coenzyme a: K26 (≠ E26), A27 (≠ V27), L28 (≠ R28), A30 (= A30), A66 (= A66), A68 (= A68), D69 (= D69), I70 (≠ L70), Y107 (≠ F107), G110 (= G110), G111 (= G111), E114 (= E114), P133 (= P133), E134 (= E134), L137 (≠ V137), G142 (= G142), F233 (≠ L233), F249 (= F249)
1ey3A Structure of enoyl-coa hydratase complexed with the substrate dac-coa (see paper)
44% identity, 97% coverage: 6:257/260 of query aligns to 2:255/258 of 1ey3A
- active site: A66 (= A68), M71 (≠ F73), S81 (≠ R83), L85 (≠ E87), G109 (= G111), E112 (= E114), P131 (= P133), E132 (= E134), T137 (≠ I139), P139 (= P141), G140 (= G142), K225 (≠ A227), F235 (= F237)
- binding 4-(n,n-dimethylamino)cinnamoyl-coa: K24 (≠ E26), L26 (≠ R28), A28 (= A30), A64 (= A66), G65 (= G67), A66 (= A68), D67 (= D69), I68 (≠ L70), L85 (≠ E87), W88 (= W90), G109 (= G111), P131 (= P133), L135 (≠ V137), G140 (= G142)
P14604 Enoyl-CoA hydratase, mitochondrial; mECH; mECH1; Enoyl-CoA hydratase 1; ECHS1; Short-chain enoyl-CoA hydratase; SCEH; EC 4.2.1.17; EC 5.3.3.8 from Rattus norvegicus (Rat) (see 3 papers)
44% identity, 97% coverage: 6:257/260 of query aligns to 34:287/290 of P14604
- E144 (= E114) mutation to D: Reduces activity 50-fold.; mutation to Q: Reduces activity 3300-fold.
- E164 (= E134) mutation to D: Reduces activity 1250-fold.; mutation to Q: Reduces activity 330000-fold.
Sites not aligning to the query:
- 1:29 modified: transit peptide, Mitochondrion
1mj3A Crystal structure analysis of rat enoyl-coa hydratase in complex with hexadienoyl-coa (see paper)
44% identity, 97% coverage: 6:257/260 of query aligns to 4:255/258 of 1mj3A
- active site: A68 (= A68), M73 (≠ F73), S83 (≠ R83), L85 (≠ R85), G109 (= G111), E112 (= E114), P131 (= P133), E132 (= E134), T137 (≠ I139), P139 (= P141), G140 (= G142), K225 (≠ A227), F235 (= F237)
- binding hexanoyl-coenzyme a: K26 (≠ E26), A27 (≠ V27), L28 (≠ R28), A30 (= A30), A66 (= A66), G67 (= G67), A68 (= A68), D69 (= D69), I70 (≠ L70), G109 (= G111), P131 (= P133), E132 (= E134), L135 (≠ V137), G140 (= G142)
2hw5C The crystal structure of human enoyl-coenzyme a (coa) hydratase short chain 1, echs1
43% identity, 94% coverage: 14:257/260 of query aligns to 14:257/260 of 2hw5C
- active site: A68 (= A68), M73 (≠ F73), S83 (≠ R83), L87 (≠ E87), G111 (= G111), E114 (= E114), P133 (= P133), E134 (= E134), T139 (≠ I139), P141 (= P141), G142 (= G142), K227 (≠ A227), F237 (= F237)
- binding crotonyl coenzyme a: K26 (≠ E26), A27 (≠ V27), L28 (≠ R28), A30 (= A30), K62 (≠ E62), I70 (≠ L70), F109 (≠ L109)
2dubA Enoyl-coa hydratase complexed with octanoyl-coa (see paper)
43% identity, 97% coverage: 6:257/260 of query aligns to 3:251/254 of 2dubA
- active site: A67 (= A68), M72 (≠ F73), S82 (≠ K88), G105 (= G111), E108 (= E114), P127 (= P133), E128 (= E134), T133 (≠ I139), P135 (= P141), G136 (= G142), K221 (≠ A227), F231 (= F237)
- binding octanoyl-coenzyme a: K25 (≠ E26), A26 (≠ V27), L27 (≠ R28), A29 (= A30), A65 (= A66), A67 (= A68), D68 (= D69), I69 (≠ L70), K70 (= K71), G105 (= G111), E108 (= E114), P127 (= P133), E128 (= E134), G136 (= G142), A137 (= A143)
5zaiC Crystal structure of 3-hydroxypropionyl-coa dehydratase from metallosphaera sedula (see paper)
38% identity, 94% coverage: 17:260/260 of query aligns to 14:259/259 of 5zaiC
- active site: A65 (= A68), F70 (= F73), S82 (≠ A82), R86 (≠ E87), G110 (= G111), E113 (= E114), P132 (= P133), E133 (= E134), I138 (= I139), P140 (= P141), G141 (= G142), A226 (= A227), F236 (= F237)
- binding coenzyme a: K24 (≠ V27), L25 (≠ R28), A63 (= A66), G64 (= G67), A65 (= A68), D66 (= D69), I67 (≠ L70), P132 (= P133), R166 (≠ P167), F248 (= F249), K251 (= K252)
5jbxB Crystal structure of liuc in complex with coenzyme a and malonic acid (see paper)
41% identity, 89% coverage: 28:259/260 of query aligns to 26:260/261 of 5jbxB
- active site: A67 (= A68), R72 (vs. gap), L84 (≠ R83), R88 (≠ E87), G112 (= G111), E115 (= E114), T134 (≠ P133), E135 (= E134), I140 (= I139), P142 (= P141), G143 (= G142), A228 (= A227), L238 (≠ F237)
- binding coenzyme a: R26 (= R28), A28 (= A30), A65 (= A66), D68 (= D69), L69 (= L70), K70 (= K71), L110 (= L109), G111 (= G110), T134 (≠ P133), E135 (= E134), L138 (≠ V137), R168 (≠ P167)
Sites not aligning to the query:
Q4WF54 Mevalonyl-coenzyme A hydratase sidH; Siderophore biosynthesis protein H; EC 4.2.1.- from Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293) (Neosartorya fumigata) (see paper)
36% identity, 93% coverage: 17:257/260 of query aligns to 21:265/270 of Q4WF54
Sites not aligning to the query:
- 268:270 PTS1-type peroxisomal targeting signal
6slbAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
36% identity, 97% coverage: 8:259/260 of query aligns to 5:256/257 of 6slbAAA
- active site: Q64 (≠ A68), F69 (= F73), L80 (≠ R83), N84 (≠ E87), A108 (≠ G111), S111 (≠ E114), A130 (≠ P133), F131 (≠ E134), L136 (≠ I139), P138 (= P141), D139 (≠ G142), A224 (= A227), G234 (≠ F237)
- binding (~{E})-6-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-6-oxidanylidene-hex-3-enoic acid: R58 (≠ E62), A62 (= A66), Q64 (≠ A68), D65 (= D69), L66 (= L70), Y76 (≠ V79), A108 (≠ G111), F131 (≠ E134), D139 (≠ G142)
6slaAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
36% identity, 97% coverage: 8:259/260 of query aligns to 2:244/245 of 6slaAAA
- active site: Q61 (≠ A68), L68 (≠ A75), N72 (≠ E87), A96 (≠ G111), S99 (≠ E114), A118 (≠ P133), F119 (≠ E134), L124 (≠ I139), P126 (= P141), N127 (≠ G142), A212 (= A227), G222 (≠ F237)
- binding ~{S}-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyl] 2-(2,5-dihydrooxepin-7-yl)ethanethioate: L21 (≠ R28), A59 (= A66), Q61 (≠ A68), D62 (= D69), L63 (= L70), L68 (≠ A75), Y71 (≠ S86), A94 (≠ L109), G95 (= G110), A96 (≠ G111), F119 (≠ E134), I122 (≠ V137), L124 (≠ I139), N127 (≠ G142), F234 (= F249), K237 (= K252)
Q7CQ56 1,4-dihydroxy-2-naphthoyl-CoA synthase; DHNA-CoA synthase; EC 4.1.3.36 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
31% identity, 97% coverage: 8:258/260 of query aligns to 25:279/285 of Q7CQ56
4elxA Structure of apo e.Coli. 1,4-dihydroxy-2- naphthoyl coa synthases with cl (see paper)
30% identity, 99% coverage: 2:258/260 of query aligns to 16:262/268 of 4elxA
- active site: G83 (≠ A68), H88 (≠ F73), L92 (≠ V79), G116 (= G111), V119 (≠ E114), G139 (≠ E134), S144 (≠ I139), D146 (≠ P141), G147 (= G142), A233 (≠ E229), Y241 (≠ F237)
- binding chloride ion: G115 (= G110), G139 (≠ E134), W167 (≠ C162)
O53561 Enoyl-CoA hydratase EchA19; EC 4.2.1.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
35% identity, 93% coverage: 17:257/260 of query aligns to 18:263/266 of O53561
- K135 (≠ T129) modified: N6-succinyllysine; mutation to E: Nearly wild-type levels of succinylation in vitro, reduces specific activity 8-fold.
- 135:142 (vs. 129:136, 38% identical) mutation to EFGISEAE: Very low levels of succinylation in vitro, reduces specific activity 15-fold.
- K142 (≠ R136) modified: N6-succinyllysine; mutation to E: About 50% succinylation in vitro, reduces specific activity 7-fold.
3h02A 2.15 angstrom resolution crystal structure of naphthoate synthase from salmonella typhimurium.
32% identity, 95% coverage: 11:258/260 of query aligns to 24:260/266 of 3h02A
- active site: G82 (≠ A68), H86 (≠ E72), L90 (≠ V79), G114 (= G111), V117 (≠ E114), G137 (≠ E134), S142 (≠ I139), D144 (≠ P141), G145 (= G142), A231 (≠ E229), Y239 (≠ F237)
- binding bicarbonate ion: G113 (= G110), Q135 (= Q132), G137 (≠ E134), W165 (≠ C162)
4elwA Structure of e. Coli. 1,4-dihydroxy-2- naphthoyl coenzyme a synthases (menb) in complex with nitrate (see paper)
30% identity, 99% coverage: 2:258/260 of query aligns to 16:261/267 of 4elwA
- active site: G83 (≠ A68), L91 (≠ V79), G115 (= G111), V118 (≠ E114), G138 (≠ E134), S143 (≠ I139), D145 (≠ P141), G146 (= G142), A232 (≠ E229), Y240 (≠ F237)
- binding nitrate ion: G114 (= G110), T137 (≠ P133), G138 (≠ E134), F144 (≠ M140), W166 (≠ C162)
Query Sequence
>WP_085123321.1 NCBI__GCF_900177295.1:WP_085123321.1
MTDASQPVLSERIDEAVVLVRINRGEVRNALNTATRKALAEAFAACHDDESVRAIVLTGN
EEAFAAGADLKEFMAAGAVDIARGRSEKYWKTIMATPQPIIAAVNGFALGGGMELAMMAD
IIVAGEGATFGQPEVRVGIMPGAGGTQRLTRAVGKYNAMRLCLTGKPIDAAEAYRIGLVS
QLVPDAEVLATALDMARSLARLPPLALQATKEAILHSENTSLEAGLAMERRALQVLFASR
DKNEGMTAFFEKRRPSFTGE
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory