SitesBLAST

165:174 (vs. 131:140, 90% identical) binding FAD
S174 (= S140) binding substrate
WIT 198:200 (= WIT 164:166) binding FAD
SR 222:223 (≠ -R 187) binding substrate
G250 (= G214) to A: in IVA; uncertain significance
Y277 (≠ R241) binding substrate
DLER 284:287 (≠ DYER 248:251) binding substrate
E286 (= E250) active site, Proton acceptor; mutation to D: Residual isovaleryl-CoA dehydrogenase activity.; mutation to G: Loss of isovaleryl-CoA dehydrogenase activity. Does not affect isovaleryl-CoA dehydrogenase activity; when associated with 407-E.; mutation to Q: Loss of isovaleryl-CoA dehydrogenase activity.
A291 (= A255) to V: in IVA; uncertain significance; dbSNP:rs886042098
R312 (= R276) binding FAD
Q323 (= Q287) binding FAD
I379 (= I343) to T: in IVA; uncertain significance
QCFGG 380:384 (≠ QLLGG 344:348) binding FAD
R398 (= R362) to Q: in IVA; uncertain significance; dbSNP:rs1477527791
Y403 (= Y367) to N: in IVA; uncertain significance
A407 (= A371) mutation to E: Does not affect isovaleryl-CoA dehydrogenase activity; when associated with 286-D.
AG 407:408 (= AG 371:372) binding substrate
TSE 409:411 (= TSE 373:375) binding FAD

Sites not aligning to the query:

1:32 modified: transit peptide, Mitochondrion

5lnxD Crystal structure of mmgc, an acyl-coa dehydrogenase from bacillus subtilis.
44% identity, 95% coverage: 15:386/390 of query aligns to 4:373/374 of 5lnxD

query
sites
5lnxD

E

Q

E

E

A

Q

D

V

L

M

R

R

D

K

T

M

V

V

M

R

S

D

F

F

S

A

Q

R

E

K

R

E

I

I

A

A

P

P

L

A

A

A

E

E

R

I

I

M

D

E

R

K

E

T

D

D

W

E

F

F

P

P

R

F

E

Q

L

L

W

I

P

K

Q

K

L

M

G

G

A

K

L

H

G

G

L

L

H

M

G

G

I

I

T

P

V

V

E

P

E

E

E

Q

Y

Y

G

G

S

A

G

G

L

A

G

D

Y

V

L

V

H

S

H

Y

C

I

V

L

A

A

M

I

E

H

E

E

V

I

S

S

R

R

A

I

S

S

A

A

S

A

V

V

G

G

L

V

S

I

Y

L

G

S

A

V

H

H

S

T

N

S

L

V

C

G

V

T

N

N

Q

P

I

I

R

L

R

Y

N

F

G

G

S

N

E

E

D

E

Q

Q

K

K

R

M

R

K

Y

Y

L

I

P

P

K

N

L

L

V

A

S

S

G

G

E

D

H

H

V

L

G

G

S

A

L

F

A

A

M
x
L

S
x
T

E

E

P

P

G

H

A

S

G
|
G

S
|
S

D

D

V

A

V

G

S

S

M

L

K

R

T

T

R

T

A

A

E

I

K

K

R

K

G

N

D

G

R

K

W

Y

I

L

L

L

N

N

G

G

G

S

K

K

M

I

W
x
F

I

I

T
|
T

N

N

A

G

S

G

E

A

A

A

E

D

T

I

L

Y

V

I

I

T

Y

F

A

A

K

L

T

T

D

A

P

P

D

D

A

Q

G

G

P

R

R

H

G

G

I

I

T

S

A

A

F

F

I

I

V

V

E

E

K

K

G

N

F

T

K

P

G

G

F

F

S

T

I

V

A

G

Q

K

K

K

L

E

D

R

K

K

L

L

G

G

M

L

R

Y

G

G

S

S

P

N

T

T

C

T

E

E

L

L

V

I

F

F

E

D

D

N

C

A

E

E

V

V

P

P

E

E

E

A

N

N

V

L

L

L

G

G

E

K

V

E

G

G

K

D

G

G

V

F

R

H

V

I

L

A

M

M

S

A

G

N

L

L

D

N

Y

V

E
x
G

R

R

A

I

V

G

L

I

A

A

G

Q

P

A

L

L

G

G

I

I

M

A

Q

E

A

A

L

L

D

E

V

H

A

A

I

V

P

D

Y

Y

V

A

H

K

E

Q

R
|
R

K

V

Q
|
Q

F
|
F

G

G

Q

R

P

P

I
|
I

G

A

A

A

F
x
N

Q

Q

L

G

V
x
I

Q

S

G

F

K

K

L

L

A

A

D

D

M

M

Y

A

T

T

T

R

A

A

N

E

A

A

C

A

R

R

A

H

Y

L

V

V

Y

Y

A

H

V

A

A

A

Q

D

A

L

C

H

D

N

R

R

G

-

E

L

T

N

T

C

R

G

K

K

D

E

A

A

A

S

G

M

A

A

I

K

L

Q

Y

F

A

A

A

S

E

D

K

A

A

A

T

V

Q

K

V

-

A

A

L

L

D

D

A

A

I

V

Q
|
Q

L
x
I

L

Y

G

G

G
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G

N

Y

G

G

Y

Y

I

M

N

K

D

D

Y

Y

P

P

T

V

G

E

R

R

L

L

R

R

D

D

A

A

K

K

L

V

Y

T

E

Q

I

I

G
x
Y

A
x
E

G

G

T
|
T

S

N

E
|
E

I

I

R

Q

R

R

W

L

L

I

I

I

G

S

R
x
K

E

Y

L

L

F

L

active site: L122 (≠ M133), T123 (≠ S134), G239 (≠ E250), E358 (≠ A371), K370 (≠ R383)
binding flavin-adenine dinucleotide: L122 (≠ M133), T123 (≠ S134), G128 (= G139), S129 (= S140), F153 (≠ W164), T155 (= T166), R265 (= R276), Q267 (= Q278), F268 (= F279), I272 (= I283), N275 (≠ F286), I278 (≠ V289), Q331 (= Q344), I332 (≠ L345), G335 (= G348), Y357 (≠ G370), T360 (= T373), E362 (= E375)

5ol2F The electron transferring flavoprotein/butyryl-coa dehydrogenase complex from clostridium difficile (see paper)
44% identity, 95% coverage: 19:387/390 of query aligns to 10:378/378 of 5ol2F

query
sites
5ol2F

L

M

L

L

R

K

D

E

T

L

V

Y

M

V

S

S

F

F

S

A

Q

E

E

N

R

E

I

V

A

K

P

P

L

L

A

A

E

T

R
x
E

I

L

D

D

R

E

E
|
E

D

E

W
x
R

F

F

P

P

R

Y

E

E

L

T

W

V

P

E

Q

K

L

M

G

A

A

K

L

A

G

G

L

M

H

M

G

G

I

I

T

P

V

Y

E

P

E

K

E

E

Y

Y

G

G

S

E

G

G

L

G

G

D

Y

T

L

V

H

G

H

Y

C

I

V

M

A

A

M

V

E

E

V

L

S

S

R

R

A

V

S

C

A

G

S

T

V

T

G

G

L

V

S

I

Y

L

G

S

A

A

H

H

S

T

N

S

L

L

C

G

V

S

N

W

Q

P

I

I

R

Y

R

Q

N

Y

G

G

S

N

E

E

D

E

Q

Q

K

K

R

Q

R

K

Y

F

L

L

P

R

K

P

L

L

V

A

S

S

G

G

E

E

H

K

V

L

G

G

S

A

L
x
F

A

G

M
x
L

S
x
T

E

E

P
|
P

G

N

A

A

G

G

S
x
T

D

D

V

A

V

S

S

G

M

Q

K

Q

T

T

R

T

A

A

E

V

K

L

R

D

G

G

D

D

R

E

W

Y

I

I

L

L

N

N

G

G

G

S

K

K

M

I

W
x
F

I
|
I

T
|
T

N

N

A

A

S

I

E

A

A

G

E

D

T

I

L

Y

V

V

I

V

Y

M

A

A

K

M

T

T

D

D

P

K

D

S

A

K

G

G

P

N

R

K

G

G

I

I

T

S

A

A

F

F

I

I

V

V

E

E

K

K

G

G

F

T

K

P

G

G

F

F

S

S

I

F

A

G

Q

V

K

K

L
x
E

D

K

K

K

L

M

G

G

M

I

R

R

G

G

S

S

P

A

T

T

C

S

E

E

L

L

V

I

F

F

E

E

D

D

C

C

E

R

V

I

P

P

E

K

E

E

N

N

V

L

L

L

G

G

E

K

V

E

G

G

K

Q

G

G

V

F

R

K

V

I

L

A

M

M

S

S

G

T

L
|
L

D

D

Y

G

E
x
G

R
|
R

A

I

V

G

L

I

A

A

G

Q

P

A

L

L

G

G

I

L

M

A

Q

Q

A

G

A

A

L

L

D

D

V

E

A

T

I

V

P

K

Y

Y

V

V

H

K

E

E

R
|
R

K

V

Q

Q

F
|
F

G

G

Q

R

P

P

I
x
L

G

S

A

K

F
|
F

Q

Q

L

N

V

T

Q

Q

G

F

K

Q

L

L

A

A

D

D

M

M

Y

E

T

V

T

K

A

V

N

Q

A

A

C

A

R

R

A

H

Y

L

V

V

Y

Y

A

Q

V

A

A

A

Q

I

A

N

C

K

D

D

R

L

G

G

E

K

T

P

T

Y

R

G

K

V

D

E

A

A

G

M

A

A

I

K

L

L

Y

F

A

A

E

E

K

T

A

A

T

M

Q

E

V

V

A

T

L

T

D

K

A

A

I

V

Q
|
Q

L
|
L

L

H

G
|
G

N

Y

G

G

Y

Y

I

T

N

R

D

D

Y

Y

P

P

T

V

G

E

R

R

L

M

R

R

D

D

A

A

K

K

L

I

Y

T

E

E

I

I

G
x
Y

A
x
E

G
|
G

T
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T

S

S

E
|
E

I

V

R

Q

R

R

W

M

L

V

I

I

G

S

R
x
G

E

K

L

L

F

L

E

K

active site: L124 (≠ M133), T125 (≠ S134), G241 (≠ E250), G374 (≠ R383)
binding calcium ion: E29 (≠ R38), E33 (= E42), R35 (≠ W44)
binding coenzyme a persulfide: L238 (= L247), R242 (= R251), E362 (≠ A371), G363 (= G372)
binding flavin-adenine dinucleotide: F122 (≠ L131), L124 (≠ M133), T125 (≠ S134), P127 (= P136), T131 (≠ S140), F155 (≠ W164), I156 (= I165), T157 (= T166), E198 (≠ L207), R267 (= R276), F270 (= F279), L274 (≠ I283), F277 (= F286), Q335 (= Q344), L336 (= L345), G338 (= G347), G339 (= G348), Y361 (≠ G370), T364 (= T373), E366 (= E375)

4l1fA Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
41% identity, 97% coverage: 9:388/390 of query aligns to 1:380/380 of 4l1fA

query
sites
4l1fA

L

M

N

D

F

F

G

N

L
|
L

G

T

E

E

E

D

A

Q

D
x
Q

L

M

L

I

R

K

D

D

T

M

V

A

M

A

S

E

F

F

S

A

Q

E

E

K

R

F

I

L

A

A

P

P

L

T

A

V

E

E

R

E

I

R

D

D

R

K

E

A

D

H

W

I

F

W

P

D

R

R

E

K

L

L

W

I

P

D

Q

K

L

M

G

G

A

E

L

A

G

G

L

F

H

C

G

G

I

I

T

C

V

F

E

P

E

E

Y

Y

G

G

S

M

G

G

L

L

G

D

Y

V

L

L

H

S

H

Y

C

I

V

L

A

A

M

V

E

E

V

L

S

S

R

K

A

V

S

D

A

D

S

G

V

T

G

G

L

I

S

T

Y

L

G

S

A

A

H

N

S

V

N

S

L

L

C

C

V

A

N

T

Q

P

I

I

R

Y

R

M

N

F

G

G

S

T

E

E

D

E

Q

Q

K

K

R

Q

R

K

Y

Y

L

L

P

A

K

P

L

I

V

A

S

E

G

G

E

T

H

H

V

V

G

G

S

A

L
x
F

A

G

M
x
L

S
x
T

E

E

P

P

G

S

A

A

G
|
G

S
x
T

D

D

V

A

V

S

S

A

M

Q

K

Q

T

T

R

T

A

A

E

V

K

L

R

K

G

G

D

D

R

K

W

Y

I

I

L

L

N

N

G

G

G

S

K

K

M

I

W
x
F

I
|
I

T
|
T

N

N

A

G

S

K

E

E

A

A

E

D

T

T

L

Y

V

V

I

V

Y

F

A

A

K

M

T

T

D

D

P

K

D

S

A

Q

G

G

P

V

R
x
H

G

G

I

I

T

S

A

A

F

F

I

I

V

L

E

E

K

K

G

G

F

M

K

P

G

G

F

F

S

R

I

F

A

G

Q

K

K

I

L

E

D

D

K

K

L

M

G

G

M

G

R

H

G

T

S

S

P

I

T

T

C

A

E

E

L

L

V

I

F

F

E

E

D

D

C

C

E

E

V

V

P

P

E

K

E

E

N

N

V

L

L

L

G

G

E

K

V

E

G

G

K

E

G

G

V
x
F

R

K

V

I

L

A

M
|
M

S
x
E

G

T

L
|
L

D
|
D

Y

G

E
x
G

R
|
R

A

I

V

G

L

V

A

A

G

Q

P

A

L

L

G

G

I

I

M

A

Q

E

A

G

A

A

L

L

D

A

V

A

A

A

I

V

P

K

Y

Y

V

S

H

K

E

E

R
|
R

K

E

Q
|
Q

F
|
F

G

G

Q

R

P

S

I
|
I

G

S

A

K

F
|
F

Q

Q

L

A

V
x
L

Q

Q

G

F

K

M

L

M

A

A

D

D

M

M

Y

A

T

T

T

K

A

I

N

E

A

A

C

A

R

R

A

Y

Y

L

V

V

Y

Y

A

H

V

A

A

A

Q

M

A

L

C

K

D

N

R

E

G

G

E

K

T

P

T

Y

R

S

K

E

D

A

A

A

G

M

A

A

I

K

L

C

Y

F

A

A

A

S

E

D

K

V

A

A

T

M

Q

E

V

V

A

T

L

T

D

D

A

A

I

V

Q
|
Q

L
x
I

L

F

G

G

G
|
G

N

Y

G

G

Y

Y

I

T

N

V

D

D

Y

Y

P

P

T

A

G

E

R

R

L

Y

L

M

R

R

D

N

A

A

K

K

L
x
I

Y

T

E

Q

I

I

G
x
Y

A
x
E

G
|
G

T
|
T

S

N

E
x
Q

I

V

R

M

R

R

W

I

L

V

I

T

G

S

R
|
R

E

A

L

L

F

L

E

R

E

D

active site: L125 (≠ M133), T126 (≠ S134), G242 (≠ E250), E363 (≠ A371), R375 (= R383)
binding coenzyme a persulfide: T132 (≠ S140), H179 (≠ R187), F232 (≠ V240), M236 (= M244), E237 (≠ S245), L239 (= L247), D240 (= D248), R243 (= R251), Y362 (≠ G370), E363 (≠ A371), G364 (= G372), R375 (= R383)
binding flavin-adenine dinucleotide: F123 (≠ L131), L125 (≠ M133), T126 (≠ S134), G131 (= G139), T132 (≠ S140), F156 (≠ W164), I157 (= I165), T158 (= T166), R268 (= R276), Q270 (= Q278), F271 (= F279), I275 (= I283), F278 (= F286), L281 (≠ V289), Q336 (= Q344), I337 (≠ L345), G340 (= G348), I358 (≠ L366), Y362 (≠ G370), T365 (= T373), Q367 (≠ E375)
binding 1,3-propandiol: L5 (= L13), Q10 (≠ D18)

7y0bA Crystal structure of human short-chain acyl-coa dehydrogenase
42% identity, 96% coverage: 13:386/390 of query aligns to 7:380/385 of 7y0bA

query
sites
7y0bA

L

L

G

P

E

E

E

T

A

H

D

Q

L

M

L

L

R

L

D

Q

T

T

V

C

M

R

S

D

F

F

S

A

Q

E

E

K

R

E

I

L

A

F

P

P

L

I

A

A

E

A

R

Q

I

V

D

D

R

K

E

E

D

H

W

L

F

F

P

P

R

A

E

A

L

Q

W

V

P

K

Q

K

L

M

G

G

A

G

L

L

G

G

L

L

H

L

G

A

I

M

T

D

V

V

E

P

E

E

Y

L

G

G

S

A

G

G

L

L

G

D

Y

Y

L

L

H

A

H

Y

C

A

V

I

A

A

M

M

E

E

V

I

S

S

R

R

A

G

S

C

A

A

S

S

V

T

G

G

L

V

S

I

Y

M

G

S

A

V

H

N

S

N

N

S

L

L

C

Y

V

L

N

G

Q

P

I

I

R

L

R

K

N

F

G

G

S

S

E

K

D

E

Q

Q

K

K

R

Q

R

A

Y

W

L

V

P

T

K

P

L

F

V

T

S

S

G

G

E

D

H

K

V

I

G

G

S

C

L
x
F

A

A

M
x
L

S
|
S

E

E

P

P

G

G

A

N

G
|
G

S
|
S

D

D

V

A

V

G

S

A

M

A

K

S

T

T

R

T

A

A

E

R

K

A

R

E

G

G

D

D

R

S

W

W

I

V

L

L

N

N

G

G

G

T

K

K

M

A

W
|
W

I

I

T
|
T

N

N

A

A

S

W

E

E

A

A

E

S

T

A

L

A

V

V

I

V

Y

F

A

A

K

S

T

T

D

D

P

R

D

A

A

L

G

Q

P

N

R

K

G

G

I

I

T

S

A

A

F

F

I

L

V

V

E

P

K

M

G

P

F

T

K

P

G

G

F

L

S

T

I

L

A

G

Q

K

K

K

L

E

D

D

K

K

L

L

G

G

M

I

R

R

G

G

S

S

P

S

T

T

C

A

E

N

L

L

V

I

F

F

E

E

D

D

C

C

E

R

V

I

P

P

E

K

E

D

N

S

V

I

L

L

G

G

E

E

V

P

G

G

K

M

G

G

V

F

R

K

V

I

L

A

M

M

S

Q

G

T

L

L

D

D

Y

M

E

G

R

R

A

I

V

G

L

I

A

A

A

S

G

Q

P

A

L

L

G

G

I

I

M

A

Q

Q

A

T

A

A

L

L

D

D

V

C

A

A

I

V

P

N

Y

Y

V

A

H

E

E

N

R
|
R

K

M

Q

A

F
|
F

G

G

Q

A

P

P

I

L

G

T

A

K

F
x
L

Q

Q

L
x
V

V

I

Q

Q

G

F

K

K

L

L

A

A

D

D

M

M

Y

A

T

L

T

A

A

L

N

E

A

S

C

A

R

R

A

L

Y

L

V

T

Y

W

A

R

V

A

A

A

Q

M

A

L

C

K

D

D

R

N

G

K

E

K

T

P

T

F

R

I

K

K

D

E

A

A

G

M

A

A

I

K

L

L

Y

A

A

A

A

S

E

E

K

A

A

A

T

T

Q

A

V

I

A

S

L

H

D

Q

A

A

I

I

Q
|
Q

L
x
I

L

L

G

G

G
|
G

N
x
M

G

G

Y

Y

I

V

N
x
T

D
x
E

Y

M

P

P

T

A

G

E

R

R

L

H

L

Y

R

R

D

D

A

A

K

R

L
x
I

Y

T

E

E

I

I

G
x
Y

A

E

G

G

T
|
T

S

S

E
|
E

I
|
I

R

Q

R

R

W
x
L

L

V

I

I

G

A

R

G

E

H

L

L

F

L

binding (2S,3R,4R,5S,6R)-2-[4-chloranyl-3-[[4-[(3S)-oxolan-3-yl]oxyphenyl]methyl]phenyl]-6-(hydroxymethyl)oxane-3,4,5-triol: M343 (≠ N349), T347 (≠ N353), E348 (≠ D354)
binding flavin-adenine dinucleotide: F125 (≠ L131), L127 (≠ M133), S128 (= S134), G133 (= G139), S134 (= S140), W158 (= W164), T160 (= T166), R270 (= R276), F273 (= F279), L280 (≠ F286), V282 (≠ L288), Q338 (= Q344), I339 (≠ L345), G342 (= G348), I360 (≠ L366), Y364 (≠ G370), T367 (= T373), E369 (= E375), I370 (= I376), L373 (≠ W379)

8sgsA Human liver mitochondrial short-chain specific acyl-coa dehydrogenase (see paper)
42% identity, 96% coverage: 13:386/390 of query aligns to 4:377/381 of 8sgsA

query
sites
8sgsA

L

L

G

P

E

E

E

T

A

H

D

Q

L

M

L

L

R

L

D

Q

T

T

V

C

M

R

S

D

F

F

S

A

Q

E

E

K

R

E

I

L

A

F

P

P

L

I

A

A

E

A

R

Q

I

V

D

D

R

K

E

E

D

H

W

L

F

F

P

P

R

A

E

A

L

Q

W

V

P

K

Q

K

L

M

G

G

A

G

L

L

G

G

L

L

H

L

G

A

I

M

T

D

V

V

E

P

E

E

Y

L

G

G

S

A

G

G

L

L

G

D

Y

Y

L

L

H

A

H

Y

C

A

V

I

A

A

M

M

E

E

V

I

S

S

R

R

A

G

S

C

A

A

S

S

V

T

G

G

L

V

S

I

Y

M

G

S

A

V

H

N

S

N

N

S

L

L

C

Y

V

L

N

G

Q

P

I

I

R

L

R

K

N

F

G

G

S

S

E

K

D

E

Q

Q

K

K

R

Q

R

A

Y

W

L

V

P

T

K

P

L

F

V

T

S

S

G

G

E

D

H

K

V

I

G

G

S

C

L
x
F

A

A

M
x
L

S
|
S

E

E

P

P

G

G

A

N

G
|
G

S
|
S

D

D

V
x
A

V

G

S

A

M

A

K

S

T

T

R

T

A

A

E

R

K

A

R

E

G

G

D

D

R

S

W

W

I

V

L

L

N

N

G

G

G

T

K

K

M

A

W
|
W

I

I

T
|
T

N

N

A

A

S

W

E

E

A

A

E

S

T

A

L

A

V

V

I

V

Y

F

A

A

K

S

T

T

D

D

P

R

D

A

A

L

G

Q

P
x
N

R

K

G

G

I

I

T

S

A

A

F

F

I

L

V

V

E

P

K

M

G

P

F

T

K

P

G

G

F

L

S

T

I

L

A

G

Q

K

K

K

L

E

D

D

K

K

L

L

G

G

M

I

R

R

G

G

S

S

P

S

T

T

C

A

E

N

L

L

V

I

F

F

E

E

D

D

C

C

E

R

V

I

P

P

E

K

E

D

N

S

V

I

L

L

G

G

E

E

V

P

G

G

K

M

G

G

V
x
F

R

K

V

I

L

A

M
|
M

S

Q

G

T

L
|
L

D

D

Y

M

E

G

R

R

A

I

V

G

L

I

A

A

A

S

G

Q

P

A

L

L

G

G

I

I

M

A

Q

Q

A

T

A

A

L

L

D

D

V

C

A

A

I

V

P

N

Y

Y

V

A

H

E

E

N

R
|
R

K

M

Q

A

F
|
F

G

G

Q

A

P

P

I
x
L

G

T

A

K

F
x
L

Q

Q

L

V

V

I

Q

Q

G

F

K

K

L

L

A

A

D

D

M

M

Y

A

T

L

T

A

A

L

N

E

A

S

C

A

R

R

A

L

Y

L

V

T

Y

W

A

R

V

A

A

A

Q

M

A

L

C

K

D

D

R

N

G

K

E

K

T

P

T

F

R
x
I

K

K

D

E

A

A

G

M

A

A

I

K

L

L

Y

A

A

A

A

S

E

E

K

A

A

A

T

T

Q

A

V

I

A

S

L

H

D

Q

A

A

I

I

Q
|
Q

L
x
I

L

L

G
|
G

N

M

G

G

Y

Y

I

V

N

T

D

E

Y

M

P

P

T

A

G

E

R

R

L

H

L

Y

R

R

D

D

A

A

K

R

L
x
I

Y

T

E

E

I
|
I

G
x
Y

A
x
E

G
|
G

T
|
T

S

S

E
|
E

I

I

R

Q

R

R

W

L

L

V

I

I

G

A

R

G

E

H

L

L

F

L

binding coenzyme a: S131 (= S140), A133 (≠ V142), N177 (≠ P186), F231 (≠ V240), M235 (= M244), L238 (= L247), I312 (≠ R321), E362 (≠ A371), G363 (= G372)
binding flavin-adenine dinucleotide: F122 (≠ L131), L124 (≠ M133), S125 (= S134), G130 (= G139), S131 (= S140), W155 (= W164), T157 (= T166), R267 (= R276), F270 (= F279), L274 (≠ I283), L277 (≠ F286), Q335 (= Q344), I336 (≠ L345), G338 (= G347), G339 (= G348), I357 (≠ L366), I360 (= I369), Y361 (≠ G370), T364 (= T373), E366 (= E375)

7y0aC Crystal structure of human short-chain acyl-coa dehydrogenase
42% identity, 96% coverage: 13:386/390 of query aligns to 10:383/387 of 7y0aC

query
sites
7y0aC

L

L

G

P

E

E

E

T

A

H

D

Q

L

M

L

L

R

L

D

Q

T

T

V

C

M

R

S

D

F

F

S

A

Q

E

E

K

R

E

I

L

A

F

P

P

L

I

A

A

E

A

R

Q

I

V

D

D

R

K

E

E

D

H

W

L

F

F

P

P

R

A

E

A

L

Q

W

V

P

K

Q

K

L

M

G

G

A

G

L

L

G

G

L

L

H

L

G

A

I

M

T

D

V

V

E

P

E

E

Y

L

G

G

S

A

G

G

L

L

G

D

Y

Y

L

L

H

A

H

Y

C

A

V

I

A

A

M

M

E

E

V

I

S

S

R

R

A

G

S

C

A

A

S

S

V

T

G

G

L

V

S

I

Y

M

G

S

A

V

H

N

S

N

N

S

L

L

C

Y

V

L

N

G

Q

P

I

I

R

L

R

K

N

F

G

G

S

S

E

K

D

E

Q

Q

K

K

R

Q

R

A

Y

W

L

V

P

T

K

P

L

F

V

T

S

S

G

G

E

D

H

K

V

I

G

G

S

C

L
x
F

A

A

M
x
L

S
|
S

E

E

P

P

G

G

A

N

G
|
G

S
|
S

D

D

V

A

V

G

S

A

M

A

K

S

T

T

R

T

A

A

E

R

K

A

R

E

G

G

D

D

R

S

W

W

I

V

L

L

N

N

G

G

G

T

K

K

M

A

W
|
W

I

I

T
|
T

N

N

A

A

S

W

E

E

A

A

E

S

T

A

L

A

V

V

I

V

Y

F

A

A

K

S

T

T

D

D

P

R

D

A

A

L

G

Q

P

N

R

K

G

G

I

I

T

S

A

A

F

F

I

L

V

V

E

P

K

M

G

P

F

T

K

P

G

G

F

L

S

T

I

L

A

G

Q

K

K

K

L

E

D

D

K

K

L

L

G

G

M

I

R

R

G

G

S

S

P

S

T
|
T

C

A

E

N

L

L

V

I

F

F

E

E

D

D

C

C

E

R

V

I

P

P

E

K

E

D

N

S

V

I

L

L

G

G

E

E

V

P

G

G

K

M

G

G

V

F

R

K

V

I

L

A

M

M

S

Q

G

T

L

L

D

D

Y

M

E

G

R

R

A

I

V

G

L

I

A

A

A

S

G

Q

P

A

L

L

G

G

I

I

M

A

Q

Q

A

T

A

A

L

L

D

D

V

C

A

A

I

V

P

N

Y

Y

V

A

H

E

E

N

R
|
R

K

M

Q

A

F
|
F

G

G

Q

A

P

P

I
x
L

G

T

A

K

F
x
L

Q

Q

L
x
V

V

I

Q

Q

G

F

K

K

L

L

A

A

D

D

M

M

Y

A

T

L

T

A

A

L

N

E

A

S

C

A

R

R

A

L

Y

L

V

T

Y

W

A

R

V

A

A

A

Q

M

A

L

C

K

D

D

R

N

G

K

E

K

T

P

T

F

R

I

K

K

D

E

A

A

G

M

A

A

I

K

L

L

Y

A

A

A

A

S

E

E

K

A

A

A

T

T

Q

A

V

I

A

S

L

H

D

Q

A

A

I

I

Q
|
Q

L
x
I

L

L

G

G

G
|
G

N

M

G

G

Y

Y

I

V

N

T

D

E

Y

M

P

P

T

A

G

E

R

R

L

H

L

Y

R

R

D

D

A

A

K

R

L
x
I

Y

T

E

E

I

I

G
x
Y

A

E

G

G

T
|
T

S

S

E
|
E

I

I

R

Q

R

R

W
x
L

L

V

I

I

G

A

R

G

E

H

L

L

F

L

binding flavin-adenine dinucleotide: F128 (≠ L131), L130 (≠ M133), S131 (= S134), G136 (= G139), S137 (= S140), W161 (= W164), T163 (= T166), T214 (= T217), R273 (= R276), F276 (= F279), L280 (≠ I283), L283 (≠ F286), V285 (≠ L288), Q341 (= Q344), I342 (≠ L345), G345 (= G348), I363 (≠ L366), Y367 (≠ G370), T370 (= T373), E372 (= E375), L376 (≠ W379)

P16219 Short-chain specific acyl-CoA dehydrogenase, mitochondrial; SCAD; Butyryl-CoA dehydrogenase; EC 1.3.8.1 from Homo sapiens (Human) (see 3 papers)
42% identity, 96% coverage: 13:386/390 of query aligns to 34:407/412 of P16219

query
sites
P16219

L

L

G

P

E

E

E

T

A

H

D

Q

L

M

L

L

R

L

D

Q

T

T

V

C

M

R

S

D

F

F

S

A

Q

E

E

K

R

E

I

L

A

F

P

P

L

I

A

A

E

A

R

Q

I

V

D

D

R

K

E

E

D

H

W

L

F

F

P

P

R

A

E

A

L

Q

W

V

P

K

Q

K

L

M

G

G

A

G

L

L

G

G

L

L

H

L

G

A

I

M

T

D

V

V

E

P

E

E

Y

L

G

G

G
|
G

S

A

G

G

L

L

G

D

Y

Y

L

L

H

A

H

Y

C

A

V

I

A

A

M

M

E

E

E
|
E

V

I

S

S

R

R

A

G

S

C

A

A

S

S

V

T

G

G

L

V

S

I

Y

M

G

S

A

V

H

N

S

N

N

S

L

L

C

Y

V

L

N

G

Q

P

I

I

R

L

R

K

N

F

G

G

S

S

E

K

D

E

Q

Q

K

K

R

Q

R

A

Y

W

L

V

P

T

K

P

L

F

V

T

S

S

G

G

E

D

H

K

V

I

G

G

S

C

L
x
F

A
|
A

M
x
L

S
|
S

E
|
E

P
|
P

G
|
G

A
x
N

G
|
G

S
|
S

D

D

V

A

V

G

S

A

M

A

K

S

T

T

R

T

A

A

E
x
R

K

A

R

E

G

G

D

D

R

S

W

W

I

V

L

L

N

N

G

G

G

T

K

K

M

A

W
|
W

I
|
I

T
|
T

N

N

A

A

S

W

E

E

A
|
A

E

S

T

A

L

A

V

V

I

V

Y

F

A

A

K

S

T

T

D

D

P

R

D

A

A

L

G

Q

P

N

R

K

G
|
G

I

I

T

S

A

A

F

F

I

L

V

V

E

P

K

M

G

P

F

T

K

P

G

G

F

L

S

T

I

L

A

G

Q

K

K

K

L

E

D

D

K

K

L

L

G

G

M

I

R

R

G

G

S

S

P

S

T

T

C

A

E

N

L

L

V

I

F

F

E

E

D

D

C

C

E

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V

I

P

P

E

K

E

D

N

S

V

I

L

L

G

G

E

E

V

P

G

G

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M

G

G

V

F

R

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V

I

L

A

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Y

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A

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A

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A

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G

I

I

M

A

Q

Q

A

T

A

A

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L

D

D

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C

A

A

I

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N

Y

Y

V

A

H

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E

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R
|
R

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M

Q

A

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F

G

G

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A

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P

I

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|
Q

L

V

V

I

Q

Q

G

F

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K

L

L

A

A

D

D

M

M

Y

A

T

L

T

A

A

L

N

E

A

S

C

A

R
|
R

A

L

Y

L

V

T

Y

W

A

R

V

A

A

A

Q

M

A

L

C

K

D

D

R

N

G

K

E

K

T

P

T

F

R

I

K

K

D

E

A

A

G

M

A

A

I

K

L

L

Y

A

A

A

A
x
S

E

E

K

A

A

A

T

T

Q

A

V

I

A

S

L

H

D

Q

A

A

I

I

Q
|
Q

L
x
I

L
|
L

G
|
G

N

M

G

G

Y

Y

I

V

N

T

D

E

Y

M

P

P

T

A

G

E

R
|
R

L

H

L

Y

R

R

D

D

A

A

K

R

L

I

Y

T

E

E

I

I

G

Y

A

E

G

G

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|
T

S
|
S

E
|
E

I

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R

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L

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A

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H

L

L

F

L

G90 (= G69) to S: in ACADSD; loss of acyl-CoA dehydrogenase activity; dbSNP:rs121908005
E104 (= E83) natural variant: Missing (in ACADSD; loss of acyl-CoA dehydrogenase activity; dbSNP:rs387906308)
152:161 (vs. 131:140, 70% identical) binding in other chain
R171 (≠ E150) to W: 69% of wild-type acyl-CoA dehydrogenase activity; confers susceptibility to ethylmalonicaciduria; dbSNP:rs1800556
WIT 185:187 (= WIT 164:166) binding in other chain
A192 (= A171) to V: in ACADSD; loss of acyl-CoA dehydrogenase activity; dbSNP:rs28940874
G209 (= G188) to S: 86% of wild-type acyl-CoA dehydrogenase activity; confers susceptibility to ethylmalonicaciduria; dbSNP:rs1799958
R297 (= R276) binding FAD
Q308 (= Q287) binding in other chain
R325 (= R304) to W: in ACADSD; loss of acyl-CoA dehydrogenase activity; dbSNP:rs121908006
S353 (≠ A332) to L: in ACADSD; loss of acyl-CoA dehydrogenase activity; dbSNP:rs28941773
QILGG 365:369 (≠ QLLGG 344:348) binding FAD
R380 (= R359) to W: in ACADSD; loss of acyl-CoA dehydrogenase activity; dbSNP:rs28940875
TSE 394:396 (= TSE 373:375) binding in other chain

Sites not aligning to the query:

1:24 modified: transit peptide, Mitochondrion

1jqiA Crystal structure of rat short chain acyl-coa dehydrogenase complexed with acetoacetyl-coa (see paper)
41% identity, 96% coverage: 13:386/390 of query aligns to 7:380/384 of 1jqiA

query
sites
1jqiA

L

L

G

P

E

E

E

T

A

H

D

Q

L

M

L

L

R

R

D

Q

T

T

V

C

M

R

S

D

F

F

S

A

Q

E

E

K

R

E

I

L

A

V

P

P

L

I

A

A

E

A

R

Q

I

L

D

D

R

K

E

E

D

H

W

L

F

F

P

P

R

T

E

S

L

Q

W

V

P

K

Q

K

L

M

G

G

A

E

L

L

G

G

L

L

H

L

G

A

I

M

T

D

V

V

E

P

E

E

Y

L

G

S

G

G

S

A

G

G

L

L

G

D

Y

Y

L

L

H

A

H

Y

C

S

V

I

A

A

M

L

E

E

V

I

S

S

R

R

A

G

S

C

A

A

S

S

V

T

G

G

L

V

S

I

Y

M

G

S

A

V

H

N

S

N

N

S

L
|
L

C

Y

V

L

N

G

Q

P

I

I

R

L

R

K

N

F

G

G

S

S

E

S

D

Q

Q

Q

K

K

R

Q

Y

W

L

I

P

T

K

P

L

F

V

T

S

N

G

G

E

D

H

K

V

I

G

G

S

C

L
x
F

A

A

M
x
L

S
|
S

E

E

P

P

G

G

A

N

G
|
G

S
|
S

D

D

V

A

V

G

S

A

M

A

K

S

T

T

R

T

A

A

E

R

K

E

R

E

G

G

D

D

R

S

W

W

I

V

L

L

N

N

G

G

G

T

K

K

M

A

W
|
W

I

I

T
|
T

N

N

A

S

S

W

E

E

A

A

E

S

T

A

L

T

V

V

I

V

Y

F

A

A

K

S

T

T

D

D

P

R

D

S

A

R

G

Q

P

N

R

K

G

G

I

I

T

S

A

A

F

F

I

L

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V

E

P

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M

G

P

F

T

K

P

G

G

F

L

S

T

I

L

A

G

Q

K

K

K

L

E

D

D

K

K

L

L

G

G

M

I

R

R

G

A

S

S

P

S

T

T

C

A

E

N

L

L

V

I

F

F

E

E

D

D

C

C

E

R

V

I

P

P

E

K

E

E

N

N

V

L

L

L

G

G

E

E

V

P

G

G

K

M

G

G

V
x
F

R

K

V

I

L

A

M
|
M

S
x
Q

G

T

L
|
L

D
|
D

Y

M

E

G

R
|
R

A

I

V

G

L

I

A

A

A

S

G

Q

P

A

L

L

G

G

I

I

M

A

Q

Q

A

A

A

S

L

L

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D

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A

A

I

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K

Y

Y

V

A

H

E

E

N

R
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R

K

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Q

A

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F

G

G

Q

A

P

P

I

L

G

T

A

K

F
x
L

Q

Q

L

N

V

I

Q

Q

G

F

K

K

L

L

A

A

D

D

M

M

Y

A

T

L

T

A

A

L

N

E

A

S

C

A

R

R

A

L

Y

L

V

T

Y

W

A

R

V

A

A

A

Q

M

A

L

C

K

D

D

R

N

G

K

E

K

T

P

T

F

R

T

K

K

D

E

A

S

A

A

G

M

A

A

I

K

L

L

Y

A

A

A

A

S

E

E

K

A

A

A

T

T

Q

A

V

I

A

S

L

H

D

Q

A

A

I

I

Q
|
Q

L
x
I

L

L

G

G

G
|
G

N

M

G

G

Y

Y

I

V

N

T

D

E

Y

M

P

P

T

A

G

E

R

R

L

Y

R

R

D

D

A

A

K

R

L
x
I

Y

T

E

E

I

I

G
x
Y

A
x
E

G
|
G

T
|
T

S

S

E
|
E

I
|
I

R

Q

R

R

W

L

L

V

I

I

G

A

R
x
G

E

H

L

L

F

L

active site: G377 (≠ R383)
binding acetoacetyl-coenzyme a: L95 (= L101), F125 (≠ L131), S134 (= S140), F234 (≠ V240), M238 (= M244), Q239 (≠ S245), L241 (= L247), D242 (= D248), R245 (= R251), Y364 (≠ G370), E365 (≠ A371), G366 (= G372)
binding flavin-adenine dinucleotide: F125 (≠ L131), L127 (≠ M133), S128 (= S134), G133 (= G139), S134 (= S140), W158 (= W164), T160 (= T166), R270 (= R276), F273 (= F279), L280 (≠ F286), Q338 (= Q344), I339 (≠ L345), G342 (= G348), I360 (≠ L366), T367 (= T373), E369 (= E375), I370 (= I376)

P15651 Short-chain specific acyl-CoA dehydrogenase, mitochondrial; SCAD; Butyryl-CoA dehydrogenase; EC 1.3.8.1 from Rattus norvegicus (Rat) (see 2 papers)
41% identity, 96% coverage: 13:386/390 of query aligns to 34:407/412 of P15651

query
sites
P15651

L

L

G

P

E

E

E

T

A

H

D

Q

L

M

L

L

R

R

D

Q

T

T

V

C

M

R

S

D

F

F

S

A

Q

E

E

K

R

E

I

L

A

V

P

P

L

I

A

A

E

A

R

Q

I

L

D

D

R

K

E

E

D

H

W

L

F

F

P

P

R

T

E

S

L

Q

W

V

P

K

Q

K

L

M

G

G

A

E

L

L

G

G

L

L

H

L

G

A

I

M

T

D

V

V

E

P

E

E

Y

L

G

S

G

G

S

A

G

G

L

L

G

D

Y

Y

L

L

H

A

H

Y

C

S

V

I

A

A

M

L

E

E

V

I

S

S

R

R

A

G

S

C

A

A

S

S

V

T

G

G

L

V

S

I

Y

M

G

S

A

V

H

N

S

N

N

S

L

L

C

Y

V

L

N

G

Q

P

I

I

R

L

R

K

N

F

G

G

S

S

E

S

D

Q

Q

Q

K

K

R

Q

Y

W

L

I

P

T

K

P

L

F

V

T

S

N

G

G

E

D

H

K

V

I

G

G

S

C

L
x
F

A
|
A

M
x
L

S
|
S

E
|
E

P
|
P

G
|
G

A
x
N

G
|
G

S
|
S

D

D

V

A

V

G

S

A

M

A

K

S

T

T

R

T

A

A

E

R

K

E

R

E

G

G

D

D

R

S

W

W

I

V

L

L

N

N

G

G

G

T

K

K

M

A

W
|
W

I
|
I

T
|
T

N

N

A

S

S

W

E

E

A

A

E

S

T

A

L

T

V

V

I

V

Y

F

A

A

K

S

T

T

D

D

P

R

D

S

A

R

G

Q

P

N

R

K

G

G

I

I

T

S

A

A

F

F

I

L

V

V

E

P

K

M

G

P

F

T

K

P

G

G

F

L

S

T

I

L

A

G

Q

K

K

K

L

E

D

D

K

K

L

L

G

G

M

I

R

R

G

A

S

S

P

S

T

T

C

A

E

N

L

L

V

I

F

F

E

E

D

D

C

C

E

R

V

I

P

P

E

K

E

E

N

N

V

L

L

L

G

G

E

E

V

P

G

G

K

M

G

G

V

F

R

K

V

I

L

A

M

M

S

Q

G

T

L

L

D
|
D

Y
x
M

E
x
G

R
|
R

A

I

V

G

L

I

A

A

A

S

G

Q

P

A

L

L

G

G

I

I

M

A

Q

Q

A

A

A

S

L

L

D

D

V

C

A

A

I

V

P

K

Y

Y

V

A

H

E

E

N

R
|
R

K

H

Q

A

F

F

G

G

Q

A

P

P

I

L

G

T

A

K

F

L

Q

Q

L

N

V

I

Q

Q

G

F

K

K

L

L

A

A

D

D

M

M

Y

A

T

L

T

A

A

L

N

E

A

S

C

A

R

R

A

L

Y

L

V

T

Y

W

A

R

V

A

A

A

Q

M

A

L

C

K

D

D

R

N

G

K

E

K

T

P

T

F

R

T

K

K

D

E

A

S

A

A

G

M

A

A

I

K

L

L

Y

A

A

A

A

S

E

E

K

A

A

A

T

T

Q

A

V

I

A

S

L

H

D

Q

A

A

I

I

Q
|
Q

L
x
I

L
|
L

G
|
G

N

M

G

G

Y

Y

I

V

N

T

D

E

Y

M

P

P

T

A

G

E

R

R

L

Y

R

R

D

D

A

A

K

R

L

I

Y

T

E

E

I

I

G

Y

A
x
E

G

G

T
|
T

S
|
S

E
|
E

I

I

R

Q

R

R

W

L

L

V

I

I

G

A

R

G

E

H

L

L

F

L

152:161 (vs. 131:140, 70% identical) binding FAD
S161 (= S140) binding substrate
WIT 185:187 (= WIT 164:166) binding FAD
DMGR 269:272 (≠ DYER 248:251) binding substrate
R297 (= R276) binding FAD
QILGG 365:369 (≠ QLLGG 344:348) binding FAD
E392 (≠ A371) active site, Proton acceptor
TSE 394:396 (= TSE 373:375) binding FAD

Sites not aligning to the query:

1:24 modified: transit peptide, Mitochondrion

3pfdC Crystal structure of an acyl-coa dehydrogenase from mycobacterium thermoresistibile bound to reduced flavin adenine dinucleotide solved by combined iodide ion sad mr (see paper)
43% identity, 94% coverage: 20:386/390 of query aligns to 4:368/369 of 3pfdC

query
sites
3pfdC

L

L

R

R

D

E

T

A

V

I

M

R

S

A

F

L

S

A

Q

E

E

K

R

E

I

I

A

A

P

P

L

Y

A

A

E

A

R

E

I

V

D

D

R

E

E

K

D

A

W

R

F

F

P

P

R

E

E

E

L

A

W

L

P

A

Q

A

L

L

G

N

A

S

L

S

G

G

L

F

H

S

G

A

I

I

T

H

V

V

E

P

E

E

Y

Y

G

G

S

Q

G

G

L

A

G

D

Y

S

L

V

H

A

H

T

C

C

V

I

A

V

M

I

E

E

V

V

S

A

R

R

A

V

S

D

A

C

S

S

V

A

G

S

L

L

S

I

Y

P

G

A

A

V

H

N

S

K

N

L

L

G

C

T

V

M

N

G

Q

L

I

I

R

L

R

R

N

-

G

G

S

S

E

E

D

E

Q

L

K

K

R

K

R

Q

Y

V

L

L

P

P

K

A

L

V

V

A

S

S

G

G

E

E

H

A

V

M

G

A

S

S

L
x
Y

A

A

M
x
L

S
|
S

E

E

P

R

G

E

A

A

G
|
G

S
|
S

D

D

V

A

S

S

M

M

K

R

T

T

R

R

A

A

E

V

K

A

R

D

G

G

D

D

R

D

W

W

I

I

L

L

N

N

G

G

G

S

K

K

M

C

W
|
W

I
|
I

T
|
T

N

N

A

G

S

G

E

K

A

S

E

T

T

W

L

Y

V

T

I

V

Y

M

A

A

K

V

T

T

D

D

P

P

D

D

A

K

G

G

P

A

R

N

G

G

I

I

T

S

A

A

F

F

I

M

V

V

E

H

K

K

G

D

F

D

K

E

G

G

F

F

S

T

I

V

A

G

Q

P

K

K

L

E

D

R

K

K

L

L

G

G

M

I

R

K

G

G

S

S

P

P

T

T

C

T

E

E

L

L

V

Y

F

F

E

E

D

N

C

C

E

R

V

I

P

P

E

G

E

D

N

R

V

I

L

I

G

G

E

E

V

P

G

G

K

T

G

G

V

F

R

K

V

T

L

A

M

L

S

A

G

T

L

L

D

D

Y

H

E
x
T

R

R

A

P

V

T

L

I

A

G

A

A

G

Q

P

A

L

V

G

G

I

I

M

A

Q

Q

A

G

A

A

L

L

D

D

V

A

A

A

I

I

P

A

Y

Y

V

T

H

K

E

E

R
|
R

K

K

Q

Q

F
|
F

G

G

Q

R

P

P

I
x
V

G

S

A

D

F
x
N

Q

Q

L

G

V

V

Q

Q

G

F

K

M

L

L

A

A

D

D

M

M

Y

A

T

M

T

K

A

I

N

E

A

A

C

A

R

R

A

L

Y

M

V

V

Y

Y

A

S

V

A

A

A

Q

A

A

R

C

A

D

E

R

R

G

G

E

D

T

L

T

G

R

F

K

I

D

S

A

A

G

S

A

K

I

-

L

C

Y

F

A

A

A

S

E

D

K

V

A

A

T

M

Q

E

V

V

A

T

L

T

D

D

A

A

I

V

Q
|
Q

L
|
L

L

F

G

G

G
|
G

N

Y

G

G

Y

Y

I

T

N

Q

D

D

Y

F

P

P

T

V

G

E

R

R

L

M

R

R

D

D

A

A

K

K

L
x
I

Y

T

E

Q

I

I

G
x
Y

A
x
E

G

G

T
|
T

S

N

E
x
Q

I

I

R

Q

R

R

W

V

L

V

I

M

G

S

R
|
R

E

A

L

L

F

L

active site: L116 (≠ M133), S117 (= S134), T233 (≠ E250), E353 (≠ A371), R365 (= R383)
binding dihydroflavine-adenine dinucleotide: Y114 (≠ L131), L116 (≠ M133), S117 (= S134), G122 (= G139), S123 (= S140), W147 (= W164), I148 (= I165), T149 (= T166), R259 (= R276), F262 (= F279), V266 (≠ I283), N269 (≠ F286), Q326 (= Q344), L327 (= L345), G330 (= G348), I348 (≠ L366), Y352 (≠ G370), T355 (= T373), Q357 (≠ E375)

7w0jE Acyl-coa dehydrogenase, tfu_1647
43% identity, 96% coverage: 15:387/390 of query aligns to 9:382/382 of 7w0jE

query
sites
7w0jE

E

E

A

H

D

E

L

A

L

L

R

R

D

E

T

A

V

I

M

R

S

S

F

V

S

A

Q

E

E

D

R

K

I

I

A

A

P

P

L

H

A

A

E

A

R

D

I

V

D

D

R

E

E

Q

D

S

W

R

F

F

P

P

R

Q

E

E

L

A

W

Y

P

E

Q

A

L

L

G

R

A

A

L

S

G

D

L

F

H

H

G

A

I

P

T

H

V

V

E

A

E

E

Y

Y

G

G

S

V

G

G

L

A

G

D

Y

A

L

L

H

A

H

T

C

C

V

I

A

V

M

I

E

E

V

I

S

A

R

R

A

V

S

C

A

A

S

S

V

S

G

S

L

L

S

-

Y

I

G

P

A

A

H

V

S

N

N

K

L

L

C

G

V

S

N

M

Q

P

I

L

R

I

R

L

N

S

G

G

S

S

E

D

D

E

Q

V

K

K

R

Q

R

R

Y

Y

L

L

P

P

K

E

L

L

V

A

S

S

G

G

E

E

H

A

V

M

G

F

S

S

L

Y

A

G

M

L

S
|
S

E

E

P

R

G

E

A

A

G

G

S

S

D

D

V

T

V

A

S

S

M

M

K

R

T

T

R

R

A

A

E

V

K

R

R

D

G

G

D

D

R

D

W

W

I

I

L

L

N

N

G

G

G

Q

K

K

M

S

W
|
W

I

I

T

T

N

N

A

A

S

G

E

I

A

S

E

K

T

Y

L

Y

V

T

I

V

Y

M

A

A

K

V

T

T

D

D

P

P

D

D

A

-

G

G

P

P

R

R

G

G

-

R

-

N

I

I

T

S

A

A

F

F

I

V

V

V

E

H

K

I

G

D

F

D

K

P

G

G

F

F

S

S

I

F

A

G

Q

E

K

P

L

E

D

R

K

K

L

L

G

G

M

I

R

K

G

G

S

S

P

P

T

T

C

R

E

E

L

L

V

I

F

F

E

D

D

N

C

V

E

R

V

I

P

P

E

G

E

D

N

R

V

L

L

V

G

G

E

K

V

V

G

G

K

E

G

G

V

L

R

R

V

T

L

A

M

L

S

R

G

T

L

L

D

D

Y

H

E

T

R

R

A

V

V

T

L

I

A

G

A

A

G

Q

P

A

L

V

G

G

I

I

M

A

Q

Q

A

G

A

A

L

L

D

D

V

Y

A

A

I

L

P

G

Y

Y

V

V

H

K

E

E

R
|
R

K

K

Q
|
Q

F
|
F

G

G

Q

K

P

A

I
|
I

G

A

A

D

F
|
F

Q

Q

L

G

V
x
I

Q

Q

G

F

K

M

L

L

A

A

D

D

M

M

Y

A

T

M

T

K

A

L

N

E

A

A

C

A

R

R

A

Q

Y

M

V

V

Y

Y

A

V

V

A

A

A

Q

A

A

K

C

S

D

E

R

R

G

D

E

D

T

A

T

D

R

L

K

S

-

F

D

Y

A

G

A

A

G

A

A

A

I

K

L

C

Y

F

A

A

A

S

E

D

K

V

A

A

T

M

Q

E

V

I

A

T

L

T

D

D

A

A

I

V

Q
|
Q

L
|
L

L

L

G

G

G
|
G

N

Y

G

G

Y

Y

I

T

N

R

D

D

Y

Y

P

P

T

V

G

E

R

R

L

M

R

R

D

D

A

A

K

K

L

I

Y

T

E

Q

I

I

G
x
Y

A
x
E

G

G

T
|
T

S

N

E
x
Q

I
|
I

R

Q

R

R

W

V

L

V

I

M

G

A

R

R

E

Q

L

L

F

L

E

K

binding [[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2R,3S,4S)-5-azanyl-2,3,4-tris(oxidanyl)pentyl] hydrogen phosphate: S127 (= S134), W157 (= W164), R270 (= R276), Q272 (= Q278), F273 (= F279), I277 (= I283), F280 (= F286), I283 (≠ V289), Q339 (= Q344), L340 (= L345), G343 (= G348), Y365 (≠ G370), E366 (≠ A371), T368 (= T373), Q370 (≠ E375), I371 (= I376)

8i4rA Crystal structure of acyl-coa dehydrogenase complexed with acetyl-coa from thermobifida fusca
43% identity, 96% coverage: 15:387/390 of query aligns to 8:381/381 of 8i4rA

query
sites
8i4rA

E

E

A

H

D

E

L

A

L

L

R

R

D

E

T

A

V

I

M

R

S

S

F

V

S

A

Q

E

E

D

R

K

I

I

A

A

P

P

L

H

A

A

E

A

R

D

I

V

D

D

R

E

E

Q

D

S

W

R

F

F

P

P

R

Q

E

E

L

A

W

Y

P

E

Q

A

L

L

G

R

A

A

L

S

G

D

L

F

H

H

G

A

I

P

T

H

V

V

E

A

E

E

Y

Y

G

G

S

V

G

G

L

A

G

D

Y

A

L

L

H

A

H

T

C

C

V

I

A

V

M

I

E

E

V

I

S

A

R

R

A

V

S

C

A

A

S

S

V

S

G

S

L

L

S

-

Y

I

G

P

A

A

H

V

S

N

N

K

L

L

C

G

V

S

N

M

Q

P

I

L

R

I

R

L

N

S

G

G

S

S

E

D

D

E

Q

V

K

K

R

Q

R

R

Y

Y

L

L

P

P

K

E

L

L

V

A

S

S

G

G

E

E

H

A

V

M

G

F

S

S

L
x
Y

A

G

M
x
L

S
|
S

E

E

P

R

G

E

A

A

G
|
G

S
|
S

D

D

V
x
T

V

A

S

S

M

M

K

R

T

T

R

R

A

A

E

V

K

R

R

D

G

G

D

D

R

D

W

W

I

I

L

L

N

N

G

G

G

Q

K

K

M

S

W
|
W

I
|
I

T
|
T

N

N

A

A

S

G

E

I

A

S

E

K

T

Y

L

Y

V

T

I

V

Y

M

A

A

K

V

T

T

D

D

P

P

D

D

A

-

G

G

P

P

R

R

G

G

-
x
R

-

N

I

I

T

S

A

A

F

F

I

V

V

V

E

H

K

I

G

D

F

D

K

P

G

G

F

F

S

S

I

F

A

G

Q

E

K

P

L

E

D

R

K

K

L

L

G

G

M

I

R

K

G

G

S

S

P

P

T

T

C

R

E

E

L

L

V

I

F

F

E

D

D

N

C

V

E

R

V

I

P

P

E

G

E

D

N

R

V

L

L

V

G

G

E

K

V

V

G

G

K

E

G

G

V

L

R
|
R

V

T

L

A

M
x
L

S
x
R

G

T

L
|
L

D
|
D

Y

H

E

T

R
|
R

A

V

V

T

L

I

A

G

A

A

G

Q

P

A

L

V

G

G

I

I

M

A

Q

Q

A

G

A

A

L

L

D

D

V

Y

A

A

I

L

P

G

Y

Y

V

V

H

K

E

E

R

R

K

K

Q

Q

F

F

G

G

Q

K

P

A

I

I

G

A

A

D

F

F

Q

Q

L

G

V

I

Q

Q

G

F

K

M

L

L

A

A

D

D

M

M

Y

A

T

M

T

K

A

L

N

E

A

A

C

A

R

R

A

Q

Y

M

V

V

Y

Y

A

V

V

A

A

A

Q

A

A

K

C

S

D

E

R

R

G

D

E

D

T

A

T

D

R

L

K

S

-

F

D

Y

A

G

A

A

G

A

A

A

I

K

L

C

Y

F

A

A

A

S

E

D

K

V

A

A

T

M

Q

E

V

I

A

T

L

T

D

D

A

A

I

V

Q

Q

L

L

G

G

N

Y

G

G

Y

Y

I

T

N

R

D

D

Y

Y

P

P

T

V

G

E

R

R

L

M

R

R

D

D

A

A

K

K

L
x
I

Y

T

E

Q

I

I

G

Y

A
x
E

G
|
G

T
|
T

S

N

E
x
Q

I

I

R

Q

R

R

W

V

L

V

I

M

G

A

R
|
R

E

Q

L

L

F

L

E

K

binding acetyl coenzyme *a: S132 (= S140), T134 (≠ V142), R180 (vs. gap), R234 (= R241), L237 (≠ M244), R238 (≠ S245), L240 (= L247), D241 (= D248), R244 (= R251), E365 (≠ A371), G366 (= G372), R377 (= R383)
binding flavin-adenine dinucleotide: Y123 (≠ L131), L125 (≠ M133), S126 (= S134), G131 (= G139), S132 (= S140), W156 (= W164), I157 (= I165), T158 (= T166), I360 (≠ L366), T367 (= T373), Q369 (≠ E375)

8i4pA Crystal structure of acyl-coa dehydrogenase from thermobifida fusca
43% identity, 96% coverage: 15:387/390 of query aligns to 8:381/381 of 8i4pA

query
sites
8i4pA

E

E

A

H

D

E

L

A

L

L

R

R

D

E

T

A

V

I

M

R

S

S

F

V

S

A

Q

E

E

D

R

K

I

I

A

A

P

P

L

H

A

A

E

A

R

D

I

V

D

D

R

E

E

Q

D

S

W

R

F

F

P

P

R

Q

E

E

L

A

W

Y

P

E

Q

A

L

L

G

R

A

A

L

S

G

D

L

F

H

H

G

A

I

P

T

H

V

V

E

A

E

E

Y

Y

G

G

S

V

G

G

L

A

G

D

Y

A

L

L

H

A

H

T

C

C

V

I

A

V

M

I

E

E

V

I

S

A

R

R

A

V

S

C

A

A

S

S

V

S

G

S

L

L

S

-

Y

I

G

P

A

A

H

V

S

N

N

K

L

L

C

G

V

S

N

M

Q

P

I

L

R

I

R

L

N

S

G

G

S

S

E

D

D

E

Q

V

K

K

R

Q

R

R

Y

Y

L

L

P

P

K

E

L

L

V

A

S

S

G

G

E

E

H

A

V

M

G

F

S

S

L
x
Y

A

G

M
x
L

S
|
S

E

E

P

R

G

E

A

A

G
|
G

S
|
S

D

D

V

T

V

A

S

S

M

M

K

R

T

T

R

R

A

A

E

V

K

R

R

D

G

G

D

D

R

D

W

W

I

I

L

L

N

N

G

G

G

Q

K

K

M

S

W
|
W

I
|
I

T
|
T

N

N

A

A

S

G

E

I

A

S

E

K

T

Y

L

Y

V

T

I

V

Y

M

A

A

K

V

T

T

D

D

P

P

D

D

A

-

G

G

P

P

R

R

G

G

-

R

-

N

I

I

T

S

A

A

F

F

I

V

V

V

E

H

K

I

G

D

F

D

K

P

G

G

F

F

S

S

I

F

A

G

Q

E

K

P

L

E

D

R

K

K

L

L

G

G

M

I

R

K

G

G

S

S

P

P

T

T

C

R

E

E

L

L

V

I

F

F

E

D

D

N

C

V

E

R

V

I

P

P

E

G

E

D

N

R

V

L

L

V

G

G

E

K

V

V

G

G

K

E

G

G

V

L

R

R

V

T

L

A

M

L

S

R

G

T

L

L

D

D

Y

H

E

T

R

R

A

V

V

T

L

I

A

G

A

A

G

Q

P

A

L

V

G

G

I

I

M

A

Q

Q

A

G

A

A

L

L

D

D

V

Y

A

A

I

L

P

G

Y

Y

V

V

H

K

E

E

R

R

K

K

Q

Q

F

F

G

G

Q

K

P

A

I

I

G

A

A

D

F

F

Q

Q

L

G

V

I

Q

Q

G

F

K

M

L

L

A

A

D

D

M

M

Y

A

T

M

T

K

A

L

N

E

A

A

C

A

R

R

A

Q

Y

M

V

V

Y

Y

A

V

V

A

A

A

Q

A

A

K

C

S

D

E

R

R

G

D

E

D

T

A

T

D

R

L

K

S

-

F

D

Y

A

G

A

A

G

A

A

A

I

K

L

C

Y

F

A

A

A

S

E

D

K

V

A

A

T

M

Q

E

V

I

A

T

L

T

D

D

A

A

I

V

Q

Q

L

L

G

G

N

Y

G

G

Y

Y

I

T

N

R

D

D

Y

Y

P

P

T

V

G

E

R

R

L

M

R

R

D

D

A

A

K

K

L
x
I

Y

T

E

Q

I

I

G
x
Y

A

E

G

G

T
|
T

S

N

E
x
Q

I

I

R

Q

R

R

W

V

L

V

I

M

G

A

R

R

E

Q

L

L

F

L

E

K

binding flavin-adenine dinucleotide: Y123 (≠ L131), L125 (≠ M133), S126 (= S134), G131 (= G139), S132 (= S140), W156 (= W164), I157 (= I165), T158 (= T166), I360 (≠ L366), Y364 (≠ G370), T367 (= T373), Q369 (≠ E375)

2vigB Crystal structure of human short-chain acyl coa dehydrogenase
41% identity, 96% coverage: 13:386/390 of query aligns to 1:367/371 of 2vigB

query
sites
2vigB

L

L

G

P

E

E

E

T

A

H

D

Q

L

M

L

L

R

L

D

Q

T

T

V

C

M

R

S

D

F

F

S

A

Q

E

E

K

R

E

I

L

A

F

P

P

L

I

A

A

E

A

R

Q

I

V

D

D

R

K

E

E

D

H

W

L

F

F

P

P

R

A

E

A

L

Q

W

V

P

K

Q

K

L

M

G

G

A

G

L

L

G

G

L

L

H

L

G

A

I

M

T

D

V

V

E

P

E

E

Y

L

G

G

S

A

G

G

L

L

G

D

Y

Y

L

L

H

A

H

Y

C

A

V

I

A

A

M

M

E

E

V

I

S

S

R

R

A

G

S

C

A

A

S

S

V

T

G

G

L

V

S

I

Y

M

G

S

A

V

H

N

S

N

N

S

L

L

C

Y

V

L

N

G

Q

P

I

I

R

L

R

K

N

F

G

G

S

S

E

K

D

E

Q

Q

K

K

R

Q

R

A

Y

W

L

V

P

T

K

P

L

F

V

T

S

S

G

G

E

D

H

K

V

I

G

G

S

C

L

F

A

A

M
x
L

S
|
S

E

E

P

P

G

G

A

N

G
|
G

S
|
S

D

D

V

A

V

G

S

A

M

A

K

S

T

T

R

T

A

A

E

R

K

A

R

E

G

G

D

D

R

S

W

W

I

V

L

L

N

N

G

G

G

T

K

K

M

A

W
|
W

I

I

T
|
T

N

N

A

A

S

W

E

E

A

A

E

S

T

A

L

A

V

V

I

V

Y

F

A

A

K

S

T

T

D

S

P

-

D

-

A

-

G

-

P

-

R

-

G

-

I

I

T

S

A

A

F

F

I

L

V

V

E

P

K

M

G

P

F

T

K

P

G

G

F

L

S

T

I

L

A

G

Q

K

K

K

L

E

D

D

K

K

L

L

G

G

M

I

R

R

G

G

S

S

P

S

T

T

C

A

E

N

L

L

V

I

F

F

E

E

D

D

C

C

E

R

V

I

P

P

E

K

E

D

N

S

V

I

L

L

G

G

E

E

V

P

G

G

K

M

G

G

V
x
F

R

K

V

I

L

A

M
|
M

S
x
Q

G

T

L
|
L

D
|
D

Y

M

E
x
G

R
|
R

A

I

V

G

L

I

A

A

A

S

G

Q

P

A

L

L

G

G

I

I

M

A

Q

Q

A

T

A

A

L

L

D

D

V

C

A

A

I

V

P

N

Y

Y

V

A

H

E

E

N

R
|
R

K

M

Q

A

F
|
F

G

G

Q

A

P

P

I
x
L

G

T

A

K

F
x
L

Q

Q

L

V

V

I

Q

Q

G

F

K

K

L

L

A

A

D

D

M

M

Y

A

T

L

T

A

A

L

N

E

A

S

C

A

R

R

A

L

Y

L

V

T

Y

W

A

R

V

A

A

A

Q

M

A

L

C

K

D

D

R

N

G

K

E

K

T

P

T

F

R

I

K

K

D

E

A

A

G

M

A

A

I

K

L

L

Y

A

A

A

A

S

E

E

K

A

A

A

T

T

Q

A

V

I

A

S

L

H

D

Q

A

A

I

I

Q
|
Q

L
x
I

L

L

G

G

G
|
G

N

M

G

G

Y

Y

I

V

N

T

D

E

Y

M

P

P

T

A

G

E

R

R

L

H

L

Y

R

R

D

D

A

A

K

R

L
x
I

Y

T

E

E

I

I

G
x
Y

A
x
E

G
|
G

T
|
T

S

S

E
|
E

I
|
I

R

Q

R

R

W

L

L

V

I

I

G

A

R
x
G

E

H

L

L

F

L

active site: L121 (≠ M133), S122 (= S134), G231 (≠ E250), E352 (≠ A371), G364 (≠ R383)
binding coenzyme a persulfide: S128 (= S140), F221 (≠ V240), M225 (= M244), Q226 (≠ S245), L228 (= L247), D229 (= D248), R232 (= R251), E352 (≠ A371), G353 (= G372), I357 (= I376)
binding flavin-adenine dinucleotide: L121 (≠ M133), S122 (= S134), G127 (= G139), S128 (= S140), W152 (= W164), T154 (= T166), R257 (= R276), F260 (= F279), L264 (≠ I283), L267 (≠ F286), Q325 (= Q344), I326 (≠ L345), G329 (= G348), I347 (≠ L366), Y351 (≠ G370), T354 (= T373), E356 (= E375)

4n5fA Crystal structure of a putative acyl-coa dehydrogenase with bound fadh2 from burkholderia cenocepacia j2315
40% identity, 95% coverage: 15:385/390 of query aligns to 8:378/378 of 4n5fA

query
sites
4n5fA

E

E

E

D

A

Q

D

R

L

M

L

I

R

R

D

D

T

A

V

A

M

R

S

A

F

F

S

A

Q

T

E

E

R

M

I

L

A

A

P

P

L

N

A

A

E

A

R

Q

I

W

D

D

R

H

E

D

D

A

W

H

F

L

P

P

R

D

E

A

L

I

W

V

P

A

Q

Q

L

L

G

G

A

E

L

L

G

G

L

L

H

L

G

G

I

M

T

I

V

V

E

P

E

Q

E

E

Y

L

G

G

S

S

G

Y

L

T

G

D

Y

Y

L

V

H

A

H

Y

C

A

V

L

A

A

M

M

E

E

V

V

S

A

R

A

A

G

S

D

A

A

S

A

V

C

G

A

L

T

S

M

Y

M

G

S

A

V

H

H

S

N

N

S

L

V

C

G

V

C

N

G

Q

P

I

I

R

L

R

G

N

F

G

G

S

T

E

P

D

A

Q

Q

K

K

R

D

R

R

Y

W

L

L

P

A

K

D

L

M

V

A

S

A

G

G

E

R

H

V

V

I

G

G

S

A

L

F

A

C

M
x
L

S
x
T

E

E

P

P

G

H

A

A

G
|
G

S
|
S

D

E

V

A

V

N

S

N

M

L

K

R

T

T

R

R

A

A

E

E

K

L

R

R

G

D

D

G

R

Q

W

W

I

V

L

L

N

N

G

G

G

A

K

K

M

Q

W
x
F

I

V

T
|
T

N

N

A

G

S

Q

E

R

A

A

E

G

T

V

L

A

V

I

I

V

Y

F

A

A

K

M

T

T

D

D

P

P

D

E

A

A

G

G

P

K

R

R

G

G

I

I

T

S

A

A

F

F

I

L

V

V

E

P

K

T

G

D

F

T

K

P

G

G

F

F

S

I

I

V

A

G

Q

K

K

P

L

E

D

K

K

K

L

M

G

G

M

I

R

R

G

A

S

S

P

D

T
|
T

C

C

E

P

L

I

V

T

F

F

E

E

D

N

C

C

E

A

V

I

P

P

E

E

E

D

N

N

V

L

L

L

G

G

E

N

V

R

G

G

K

E

G

G

V

L

R

K

V

I

L

A

M

L

S

S

G

N

L

L

D

E

Y

G

E
x
G

R

R

A

I

V

G

L

I

A

A

G

Q

P

A

L

L

G

G

I

I

M

A

Q

R

A

A

L

F

D

D

V

K

A

A

I

R

P

R

Y

Y

V

A

H

G

E

E

R

R

K

V

Q

Q

F

F

G

G

Q

K

P

P

I

I

G

A

A

E

F

H

Q

Q

L

A

V

I

Q

Q

G

Q

K

K

L

L

A

A

D

D

M

M

Y

A

T

V

T

Q

A

I

N

N

A

A

C

A

R

R

A

L

Y

L

V

V

Y

H

A

H

V

A

A

A

Q

K

A

L

C

R

D

T

R

A

G

G

E

L

T

P

T

C

R

L

K

S

D

E

A

A

A

S

G

Q

A

A

I

K

L

L

Y

F

A

A

A

S

E

E

K

M

A

A

T

E

Q

R

V

V

A

C

L

S

D

D

A

A

I

I

Q

Q

L

I

L

H

G

G

N

Y

G

G

Y

Y

I

L

N

V

D

D

Y

Y

P

E

T

V

G

E

R

R

L

H

L

Y

R

R

D

D

A

A

K

R

L

I

Y

T

E

Q

I

I

G
x
Y

A
x
E

G

G

T
|
T

S

S

E
|
E

I

V

R

Q

R

R

W
x
M

L

V

I

I

G

A

R
|
R

E

Q

L

L

active site: L126 (≠ M133), T127 (≠ S134), G243 (≠ E250), E364 (≠ A371), R376 (= R383)
binding dihydroflavine-adenine dinucleotide: L126 (≠ M133), T127 (≠ S134), G132 (= G139), S133 (= S140), F157 (≠ W164), T159 (= T166), T210 (= T217), Y363 (≠ G370), T366 (= T373), E368 (= E375), M372 (≠ W379)

P45954 Short/branched chain specific acyl-CoA dehydrogenase, mitochondrial; SBCAD; 2-methyl branched chain acyl-CoA dehydrogenase; 2-MEBCAD; 2-methylbutyryl-coenzyme A dehydrogenase; 2-methylbutyryl-CoA dehydrogenase; EC 1.3.8.5 from Homo sapiens (Human) (see 6 papers)
39% identity, 95% coverage: 15:385/390 of query aligns to 59:428/432 of P45954

query
sites
P45954

E

D

E

E

A

E

D

M

L

M

L

I

R

K

D

S

T

S

V

V

M

K

S

K

F

F

S

A

Q

Q

E

E

R

Q

I

I

A

A

P

P

L

L

A

V

E

S

R

T

I

M

D

D

R

E

E

N

D

S

W

K

F

M

P

E

R

K

E

S

L

V

W

I

P

Q

Q

G

L

L

G

F

A

Q

L

Q

G

G

L

L

H

M

G

G

I

I

T

E

V

V

E

D

E

P

E

E

Y

Y

G

G

S

T

G

G

L

A

G

S

Y

F

L

L

H

S

H

T

C

V

V

L

A

V

M

I

E

E

V

L

S

A

R

K

A

V

S

D

A

A

S

S

V

V

G

A

L
x
V

S
x
F

Y

C

G

E

A

I

H

Q

S

N

N

T

L

L

C

I

V

N

N

T

Q

L

I

I

R

R

R

K

N

H

G

G

S

T

E

E

D

E

Q

Q

K

K

R

A

R

T

Y

Y

L

L

P

P

K

Q

L

L

V

T

S

T

G

-

E

E

H

K

V

V

G

G

S

S

L
x
F

A
x
C

M
x
L

S
|
S

E
|
E

P
x
A

G
|
G

A
|
A

G
|
G

S
|
S

D

D

V

S

V

F

S

A

M

L

K

K

T

T

R

R

A

A

E

D

K

K

R

E

G

G

D

D

R

Y

W

Y

I

V

L

L

N

N

G

G

G

S

K

K

M

M

W
|
W

I
|
I

T
x
S

N
x
S

A

A

S

E

E

H

A

A

E

G

T

L

L

F

V

L

I

V

Y

M

A

A

K

N

T

V

D

D

P

P

D

T

A

I

G

G

P
x
Y

R

K

G

G

I

I

T

T

A

S

F

F

I

L

V

V

E

D

K

R

G

D

F

T

K

P

G

G

F

L

S

H

I

I

A

G

Q

K

K

P

L

E

D

N

K

K

L

L

G

G

M
x
L

R

R

G

A

S

S

P

S

T

T

C

C

E

P

L

L

V

T

F

F

E

E

D

N

C

V

E

K

V

V

P

P

E

E

E

A

N

N

V

I

L

L

G

G

E

Q

V

I

G

G

K

H

G

G

V
x
Y

R

K

V

Y

L

A

M

I

S

G

G

S

L

L

D
x
N

Y
x
E

E
x
G

R
|
R

A

I

V

G

L

I

A

A

G

Q

P

M

L

L

G

G

I

L

M

A

Q

Q

A

G

A

C

L

F

D

D

V

Y

A

T

I

I

P

P

Y

Y

V
x
I

H

K

E

E

R
|
R

K

I

Q

Q

F

F

G

G

Q

K

P

R

I

L

G

F

A

D

F

F

Q
|
Q

L

G

V

L

Q

Q

G

H

K

Q

L

V

A

A

D

H

M

V

Y

A

T

T

T

Q

A

L

N

E

A

A

C

A

R

R

A

L

Y

L

V

T

Y

Y

A

N

V

A

A

A

Q

R

A

L

C

L

D

E

R

A

G

G

E

K

T

P

T

F

R

I

K

K

D

E

A

A

A

S

G

M

A

A

I

K

L

Y

Y

Y

A

A

A

S

E

E

K

I

A

A

T

G

Q

Q

V

T

A

T

L

S

D

K

A

C

I

I

Q
x
E

L
x
W

L
x
M

G
|
G

N

V

G

G

Y

Y

I

T

N

K

D

D

Y

Y

P

P

T

V

G

E

R

K

L

Y

L

F

R

R

D

D

A

A

K

K

L

I

Y

G

E

T

I

I

G

Y

A

E

G

G

T
x
A

S
|
S

E
x
N

I

I

R

Q

R

L

W

N

L

T

I

I

G

A

R

K

E

H

L

I

V137 (≠ L93) mutation to L: Decreased acyl-CoA dehydrogenase activity.
F138 (≠ S94) mutation to L: Increased acyl-CoA dehydrogenase activity. No effect on substrate specificity.
174:183 (vs. 131:140, 60% identical) binding in other chain
S183 (= S140) binding substrate
WIS 207:209 (≠ WIT 164:166) binding in other chain
S210 (≠ N167) mutation to N: Increased acyl-CoA dehydrogenase activity. Changed substrate specificity.
Y229 (≠ P186) binding substrate
L255 (≠ M212) to F: in SBCADD; loss of protein expression; loss of 2-methylbutyryl-CoA dehydrogenase activity; dbSNP:rs137852649
Y283 (≠ V240) binding substrate
NEGR 291:294 (≠ DYER 248:251) binding substrate
I316 (≠ V273) to V: in dbSNP:rs1131430
R319 (= R276) binding FAD
Q330 (= Q287) binding FAD
EWMGG 387:391 (≠ QLLGG 344:348) binding FAD
A416 (≠ T373) mutation to T: Increased acyl-CoA dehydrogenase activity. No effect on substrate specificity.
ASN 416:418 (≠ TSE 373:375) binding in other chain

Sites not aligning to the query:

1:33 modified: transit peptide, Mitochondrion
13 R → K: in dbSNP:rs12263012

Query Sequence

>WP_085124268.1 NCBI__GCF_900177295.1:WP_085124268.1
MIPNQLPSLNFGLGEEADLLRDTVMSFSQERIAPLAERIDREDWFPRELWPQLGALGLHG
ITVEEEYGGSGLGYLHHCVAMEEVSRASASVGLSYGAHSNLCVNQIRRNGSEDQKRRYLP
KLVSGEHVGSLAMSEPGAGSDVVSMKTRAEKRGDRWILNGGKMWITNASEAETLVIYAKT
DPDAGPRGITAFIVEKGFKGFSIAQKLDKLGMRGSPTCELVFEDCEVPEENVLGEVGKGV
RVLMSGLDYERAVLAAGPLGIMQAALDVAIPYVHERKQFGQPIGAFQLVQGKLADMYTTA
NACRAYVYAVAQACDRGETTRKDAAGAILYAAEKATQVALDAIQLLGGNGYINDYPTGRL
LRDAKLYEIGAGTSEIRRWLIGRELFEESA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory