SitesBLAST
Comparing WP_085125981.1 NCBI__GCF_900177295.1:WP_085125981.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
4e5tA Crystal structure of a putative mandelate racemase/muconate lactonizing enzyme (target psi-200750) from labrenzia alexandrii dfl- 11
64% identity, 98% coverage: 1:393/400 of query aligns to 1:389/392 of 4e5tA
3rcyF Crystal structure of mandelate racemase/muconate lactonizing enzyme- like protein from roseovarius sp. Tm1035
55% identity, 98% coverage: 1:391/400 of query aligns to 1:390/397 of 3rcyF
- active site: K165 (= K164), D167 (= D166), R175 (≠ F174), G208 (= G207), H210 (= H209), E234 (= E233), G259 (= G258), E260 (= E259), Q281 (= Q280), A283 (≠ N282), H310 (= H309), A313 (≠ C312), L332 (= L331), E335 (= E334)
- binding magnesium ion: E234 (= E233), E260 (= E259)
- binding alpha-D-ribofuranose: R85 (= R85), P86 (= P86), P239 (= P238), A266 (≠ Y265), E267 (= E266)
3rraB Crystal structure of enolase prk14017 (target efi-500653) from ralstonia pickettii 12j with magnesium bound
35% identity, 100% coverage: 1:400/400 of query aligns to 2:369/379 of 3rraB
- active site: I35 (≠ L43), R38 (≠ H46), Y118 (= Y128), K145 (= K164), N147 (≠ D166), E151 (≠ P170), D184 (≠ G207), H186 (= H209), E210 (= E233), G235 (= G258), E236 (= E259), R237 (= R260), Q257 (= Q280), D259 (≠ N282), H286 (= H309), P288 (≠ Y311), E311 (= E334)
- binding magnesium ion: D184 (≠ G207), E210 (= E233), E236 (= E259)
Q6BF17 D-galactonate dehydratase; GalD; EC 4.2.1.6 from Escherichia coli (strain K12)
34% identity, 93% coverage: 1:373/400 of query aligns to 1:355/382 of Q6BF17
- H185 (= H209) mutation H->N,Q: Loss of activity.
- H285 (= H309) mutation to N: Loss of activity.
- E310 (= E334) mutation to Q: Loss of activity.
4e6mA Crystal structure of putative dehydratase protein from salmonella enterica subsp. Enterica serovar typhimurium (salmonella typhimurium)
32% identity, 94% coverage: 1:375/400 of query aligns to 2:392/401 of 4e6mA
- active site: L45 (≠ Y41), G48 (≠ P44), K169 (= K164), D171 (= D166), I175 (vs. gap), E223 (≠ G207), H225 (= H209), E249 (= E233), G274 (= G258), E275 (= E259), R276 (= R260), Q296 (= Q280), D298 (≠ N282), H325 (= H309), C327 (≠ Y311), E350 (= E334), A355 (≠ L346)
- binding magnesium ion: E223 (≠ G207), E249 (= E233), E275 (= E259)
2qq6B Crystal structure of mandelate racemase/muconate lactonizing enzyme- like protein from rubrobacter xylanophilus dsm 9941
34% identity, 95% coverage: 2:381/400 of query aligns to 4:389/396 of 2qq6B
- active site: P37 (= P44), G79 (= G81), D124 (≠ Y130), K166 (= K164), D168 (= D166), D213 (≠ G207), H215 (= H209), E239 (= E233), G264 (= G258), E265 (= E259), M286 (≠ Q280), D288 (≠ N282), H315 (= H309), N316 (≠ L310), E340 (= E334), D345 (≠ H343)
- binding magnesium ion: D213 (≠ G207), E239 (= E233), E265 (= E259), H315 (= H309)
2o56A Crystal structure of a member of the enolase superfamily from salmonella typhimurium
30% identity, 95% coverage: 1:379/400 of query aligns to 2:387/392 of 2o56A
- active site: K165 (= K164), D167 (= D166), M171 (vs. gap), L186 (≠ D180), E214 (≠ G207), H216 (= H209), E240 (= E233), G265 (= G258), E266 (= E259), Q287 (= Q280), D289 (≠ N282), H316 (= H309), E341 (= E334), A346 (≠ G340)
- binding magnesium ion: E214 (≠ G207), E240 (= E233), E266 (= E259)
3sjnA Crystal structure of enolase spea_3858 (target efi-500646) from shewanella pealeana with magnesium bound
30% identity, 93% coverage: 1:372/400 of query aligns to 2:364/373 of 3sjnA
- active site: S46 (≠ C42), L49 (≠ F45), T139 (≠ Y130), K165 (= K164), G167 (≠ A168), M171 (≠ S172), D198 (≠ G207), A200 (≠ H209), E225 (= E233), I247 (≠ V255), G250 (= G258), E251 (= E259), S252 (≠ R260), Q272 (= Q280), D274 (≠ N282), H301 (= H309), G302 (≠ L310), F303 (≠ Y311), M325 (≠ L333), E326 (= E334), Q329 (= Q337), S331 (≠ W339)
- binding magnesium ion: D198 (≠ G207), E225 (= E233), E251 (= E259)
3v3wA Crystal structure of an enolase from the soil bacterium cellvibrio japonicus (target efi-502161) with bound mg and glycerol
28% identity, 99% coverage: 1:397/400 of query aligns to 1:396/397 of 3v3wA
- active site: L36 (= L43), R39 (≠ H46), H122 (≠ Y130), K144 (≠ T161), R147 (≠ K164), Q149 (≠ D166), Y159 (vs. gap), E179 (= E188), D205 (≠ G207), H207 (= H209), E231 (= E233), G256 (= G258), E257 (= E259), V258 (≠ R260), R278 (≠ Q280), T280 (≠ N282), F306 (≠ P308), H307 (= H309), G308 (vs. gap), A309 (vs. gap), E334 (= E334)
- binding magnesium ion: D205 (≠ G207), E231 (= E233), E257 (= E259)
Sites not aligning to the query:
4ihcB Crystal structure of probable mannonate dehydratase dd703_0947 (target efi-502222) from dickeya dadantii ech703
30% identity, 98% coverage: 1:393/400 of query aligns to 2:388/395 of 4ihcB
- active site: F39 (= F45), R42 (= R48), R150 (= R157), Q152 (≠ G159), D201 (≠ G207), H203 (= H209), E227 (= E233), G252 (= G258), E253 (= E259), L254 (≠ R260), R274 (≠ Q280), H276 (≠ N282), H303 (= H309), P305 (vs. gap), E330 (= E334)
- binding magnesium ion: D201 (≠ G207), E227 (= E233), E253 (= E259)
Sites not aligning to the query:
B3PDB1 D-galactonate dehydratase family member RspA; D-mannonate dehydratase; Starvation sensing protein RspA homolog; EC 4.2.1.-; EC 4.2.1.8 from Cellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa) (see paper)
28% identity, 99% coverage: 1:397/400 of query aligns to 1:401/402 of B3PDB1
- D210 (≠ G207) binding Mg(2+)
- E236 (= E233) binding Mg(2+)
- E262 (= E259) binding Mg(2+)
3p93C Crystal structure of d-mannonate dehydratase from chromohalobacter salexigens complexed with mg,d-mannonate and 2-keto-3-deoxy-d- gluconate
28% identity, 99% coverage: 1:397/400 of query aligns to 1:383/384 of 3p93C