SitesBLAST

E190 (= E206) mutation E->A,C,K,L: Reduction of transport rate.
A192 (= A208) mutation A->D,S,L: Loss of transport and MalK dissociation from the membrane.
G196 (= G212) mutation to A: No effect.; mutation to P: Loss of transport and MalK dissociation from the membrane.
P209 (= P225) mutation to A: No effect.

4ki0F Crystal structure of the maltose-binding protein/maltose transporter complex in an outward-facing conformation bound to maltohexaose (see paper)
25% identity, 62% coverage: 78:271/312 of query aligns to 234:454/490 of 4ki0F

query
sites
4ki0F

K

E

D

K

F

L

S

S

P

P

F

G

W

Y

V

T

V

V

H

T

P

T

T

G

L

W

H

K

N

N

L

F

H

T

K

R

L

V

F

F

F

T

E

D

T

E

-

G

-

I

H

Q

Y

K

P

P

-

F

-

L

-

A

I

I

W

F

L

V

W

W

N

-

T

T

M

V

V

V

V

F

S

S

V

L

V

I

A

T

T

V

F

F

L

L

S

T

L

V

F

A

C

V

A

G

V

M

C

V

A

L

G

A

Y

C

A

L

I

V

E

Q

R

W

L

E

R

A

F

L

K

R

G

G

S

K

R

A

L

V

V

Y

G

R

M

V

A

L

I

L

F

I

L

L

A

P

Y
|
Y

L

A

V

V

P

P

P

S

S

F

I

I

L

-

F

-

I

-

P

-

L

-

S

S

T

I

M

L

V

I

F

F

S

K

Y

-

G

G

L

L

F

F

D

N

S

Q

K

S

F

F

A

G

L

E

I

I

-

N

-

M

-

L

-

S

-

A

-

L

-

F

-

G

-

V

-

K

-

P

-

A

-

W

-

F

-

S

-

D

-

P

L

T

T

T

Y

A

P

R

T

T

F

M

L

L

I

I

P

I

F

V

C
x
N

T

T

W

W

L

L

-
x
G

-

Y

-

P

-
x
Y

-

M

-

I

-

L

L

C

M

M

G

G

Y

L

F

L

R

K

S

A

I

I

P

P

Y

D

E

D

L

L

E

Y

E

E

C

A

A

S

L

A

I

M

D

D

G

G

A

A

T

G

R

P

L

F

Q

Q

I

N

L

F

V

F

K

K

I

I

I

T

L

L

P

P

L

L

A

L

V

I

P

K

G

P

L

L

I

T

S

P

A

L

G

M

I

I

F

A

A
x
S

F

F

T

A

L

F

S
x
N

W

F

N

N

E

N

F

F

I

V

Y

L

A

I

L

Q

T

L

F

L

I

T

S

N

S

G

T

G

T

P

N

D

K

R

-

L

-

G

-

T

T

T

V

T

P

P

V

A

G

G

V

Y

V

T

T

D

E

L

L

L

V

V

E

N

binding alpha-D-glucopyranose: Y310 (= Y150), N361 (≠ C189), G365 (vs. gap), Y368 (vs. gap), S418 (≠ A238), N422 (≠ S242)

P02916 Maltose/maltodextrin transport system permease protein MalF from Escherichia coli (strain K12) (see 4 papers)
25% identity, 62% coverage: 78:271/312 of query aligns to 249:469/514 of P02916

query
sites
P02916

K

E

D

K

F

L

S

S

P

P

F

G

W

Y

V

T

V

V

H

T

P

T

T

G

L

W

H

K

N

N

L

F

H

T

K

R

L

V

F

F

F

T

E

D

T

E

-

G

-

I

H

Q

Y

K

P

P

-

F

-

L

-

A

I

I

W

F

L

V

W

W

N

-

T

T

M

V

V

V

V

F

S

S

V

L

V

I

A

T

T

V

F

F

L

L

S

T

L

V

F

A

C

V

A

G

V

M

C

V

A

L

G

A

Y

C

A

L

I

V

E

Q

R

W

L

E

R

A

F

L

K

R

G

G

S

K

R

A

L

V

V

Y

G

R

M

V

A

L

I

L

F

I

L

L

A

P

Y

Y

L

A

V

V

P

P

P

S

S

F

I

I

L

-

F

-

I

-

P

-

L

-

S

S

T

I

M
x
L

V

I

F

F

S

K

Y

-

G

G

L

L

F

F

D

N

S

Q

K

S

F

F

A

G

L

E

I

I

-

N

-

M

-

L

-

S

-

A

-

L

-

F

-

G

-

V

-

K

-

P

-

A

-

W

-

F

-

S

-

D

-

P

L

T

T

T

Y

A

P

R

T

T

F

M

L
|
L

I

I

P

I

F

V

C
x
N

T

T

W

W

L

L

-
x
G

-

Y

-

P

-

Y

-

M

-

I

-

L

L

C

M

M

G

G

Y

L

F

L

R

K

S

A

I

I

P

P

Y

D

E

D

L

L

E

Y

E
|
E

C

A

A
x
S

L

A

I

M

D

D

G
|
G

A

A

T

G

R

P

L

F

Q

Q

I

N

L

F

V

F

K

K

I

I

I

T

L

L

P
|
P

L

L

A

L

V

I

P

K

G

P

L

L

I

T

S

P

A

L

G

M

I

I

F

A

A

S

F

F

T

A

L

F

S

N

W

F

N

N

E

N

F

F

I

V

Y

L

A

I

L

Q

T

L

F

L

I

T

S

N

S

G

T

G

T

P

N

D

K

R

-

L

-

G

-

T

T

T

V

T

P

P

V

A

G

G

V

Y

V

T

T

D

E

L

L

L

V

V

E

N

L334 (≠ M164) mutation to W: Ability to transport lactose in a saturable manner.
L372 (= L185) mutation to W: Growth on maltose but not on media containing either maltoheptaose or maltoheptaose plus maltose.
N376 (≠ C189) mutation N->K,H: No growth on maltose.
G380 (vs. gap) mutation G->C,S: No growth on maltose.
E401 (= E206) mutation E->A,C,K,L: Reduction of transport rate.
S403 (≠ A208) mutation S->C,D,K,L: Reduction of transport rate.
G407 (= G212) mutation G->A,P: No effect.
P420 (= P225) mutation to A: No effect.

Query Sequence

>WP_085127191.1 NCBI__GCF_900177295.1:WP_085127191.1
MTDATPQRATEPGVARDGPARRGRGGDEGEGMDYLVSLPRRVVTVYLPLLVFLFVLLFPF
YWMVITTFKSNEELYDFKDFSPFWVVHPTLHNLHKLFFETHYPIWLWNTMVVSVVATFLS
LFCAVCAGYAIERLRFKGSRLVGMAIFLAYLVPPSILFIPLSTMVFSYGLFDSKFALILT
YPTFLIPFCTWLLMGYFRSIPYELEECALIDGATRLQILVKIILPLAVPGLISAGIFAFT
LSWNEFIYALTFISSTTNKTVPVGVVTELVEGDVYHWGSLMAGALMGSLPVAVLYSFFVE
YYVSSMTGAVKE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory