SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_085218115.1 NCBI__GCF_900177405.1:WP_085218115.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P22033 Methylmalonyl-CoA mutase, mitochondrial; MCM; Methylmalonyl-CoA isomerase; EC 5.4.99.2 from Homo sapiens (Human) (see 28 papers)
62% identity, 98% coverage: 1:699/714 of query aligns to 33:736/750 of P22033

query
sites
P22033

M
x
L

S
x
H

D
x
Q

K
x
Q

P
|
P

T
x
L

R
x
H

A
x
P

D
x
E

W
|
W

E
x
A

A
|
A

L
|
L

A
|
A

A
x
K

K
|
K

E
x
Q

V
x
L

K
|
K

G
|
G

R
x
K

-
x
N

-
x
P

-
x
E

D
|
D

L
|
L

T
x
I

W
|
W

H
|
H

T
|
T

P
|
P

E
|
E

G
|
G

I
|
I

A
x
S

V
x
I

K
|
K

P
|
P

L
|
L

Y
|
Y

T
x
S

A
x
K

E
x
R

D
|
D

V
x
T

A
x
M

D
|
D

I
x
L

D
x
P

P
x
E

G
x
E

L
|
L

P
|
P

G
|
G

F
x
V

A
x
K

P
|
P

F
|
F

T
|
T

R
|
R

G
|
G

V
x
P

R
x
Y

A
x
P

S
x
T

M
|
M

Y
|
Y

A
x
T

G
x
F

R
|
R

P
|
P

W
|
W

T
|
T

I
|
I

R
|
R

Q
|
Q

Y
|
Y

A
|
A

G
|
G

F
|
F

S
|
S

T
|
T

A
x
V

E
|
E

S
|
S

N
|
N

A
x
K

F
|
F

Y
|
Y

R
x
K

R
x
D

N
|
N

L
x
I

A
x
K

A
|
A

G
|
G

Q
|
Q

K
x
Q

G
|
G

L
|
L

S
|
S

V
|
V

A
|
A

F
|
F

D
|
D

L
|
L

A
|
A

T
|
T

H
|
H

R
|
R

G
|
G

Y
|
Y

D
|
D

S
|
S

D
|
D

H
x
N

P
|
P

R
|
R

V
|
V

T
x
R

G
|
G

D
|
D

V
|
V

G
|
G

K
x
M

A
|
A

G
|
G

V
|
V

A
|
A

I
|
I

D
|
D

S
x
T

V
|
V

E
|
E

D
|
D

M
x
T

K
|
K

I
|
I

L
|
L

F
|
F

D
|
D

G
|
G

I
|
I

P
|
P

L
|
L

D
x
E

K
|
K

M
|
M

S
|
S

V
|
V

S
|
S

M
|
M

T
|
T

M
|
M

N
|
N

G
|
G

A
|
A

V
|
V

I
|
I

P
|
P

I
x
V

L
|
L

A
|
A

F
x
N

F
|
F

I
|
I

V
|
V

A
x
T

G
|
G

E
|
E

Q
|
Q

G
|
G

V
|
V

A
x
P

Q
x
K

E
|
E

M
x
K

L
|
L

D
x
T

G
|
G

T
|
T

I
|
I

Q
|
Q

N
|
N

D
|
D

I
|
I

L
|
L

K
|
K

E
|
E

F
|
F

M
|
M

V
|
V

R
|
R

N
|
N

T
|
T

Y
|
Y

I
|
I

Y
x
F

P
|
P

E
|
E

P
|
P

S
|
S

M
|
M

R
x
K

I
|
I

S
x
A

D
|
D

I
|
I

F
|
F

A
x
E

F
x
Y

T
|
T

S
x
A

A
x
K

N
x
H

M
|
M

P
|
P

K
|
K

F
|
F

N
|
N

S
|
S

I
|
I

S
|
S

I
|
I

S
|
S

G
|
G

Y
|
Y

H
|
H

M
|
M

Q
|
Q

E
|
E

A
|
A

G
|
G

A
|
A

T
x
D

Q
x
A

V
x
I

Q
x
L

E
|
E

L
|
L

A
|
A

F
x
Y

T
|
T

I
x
L

A
|
A

D
|
D

G
|
G

M
x
L

E
|
E

Y
|
Y

V
x
S

K
x
R

Y
x
T

G
|
G

V
x
L

A
x
Q

S
x
A

G
|
G

L
|
L

D
x
T

I
|
I

D
|
D

R
x
E

F
|
F

A
|
A

G
x
P

R
|
R

L
|
L

S
|
S

F
|
F

F
x
W

A
x
G

I
|
I

G
|
G

M
|
M

N
|
N

F
|
F

F
x
Y

M
|
M

E
|
E

I
|
I

A
|
A

K
|
K

L
x
M

R
|
R

A
|
A

A
x
G

R
|
R

V
x
R

L
|
L

W
|
W

Y
x
A

R
x
H

V
x
L

M
x
I

T
x
E

S
x
K

L
x
M

-
x
F

G
x
Q

A
x
P

K
|
K

D
x
N

E
x
S

R
x
K

S
|
S

K
x
L

M
x
L

L
|
L

R
|
R

T
x
A

H
|
H

C
|
C

Q
|
Q

T
|
T

S
|
S

G
|
G

V
x
W

S
|
S

L
|
L

T
|
T

E
|
E

Q
|
Q

D
|
D

P
|
P

Y
|
Y

N
|
N

V
x
I

M
x
V

R
|
R

T
|
T

T
x
A

I
|
I

E
|
E

A
|
A

M
|
M

A
|
A

M
x
V

L
x
F

G
|
G

T
|
T

Q
|
Q

S
|
S

L
|
L

H
|
H

T
|
T

N
|
N

A
x
S

L
x
F

D
|
D

E
|
E

A
|
A

I
x
L

A
x
G

L
|
L

P
|
P

T
|
T

D
x
V

F
x
K

S
|
S

A
|
A

R
|
R

I
|
I

A
|
A

R
|
R

N
|
N

T
|
T

Q
|
Q

L
x
I

V
x
I

I
|
I

Q
|
Q

E
|
E

T
x
S

G
|
G

M
x
I

T
x
P

N
x
K

V
|
V

V
x
A

D
|
D

P
|
P

L
x
W

G
|
G

S
|
S

Y
|
Y

Y
x
M

V
x
M

E
|
E

A
x
C

L
|
L

T
|
T

Q
x
N

R
x
D

L
x
V

V
x
Y

D
|
D

E
x
A

A
|
A

W
x
L

A
x
K

I
x
L

I
|
I

E
x
N

R
x
E

V
x
I

E
|
E

A
x
E

E
x
M

G
|
G

M
|
M

A
|
A

K
|
K

A
|
A

V
|
V

A
|
A

A
x
E

G
|
G

W
x
I

P
|
P

K
|
K

A
x
L

M
x
R

I
|
I

E
|
E

A
x
C

A
|
A

A
x
R

A
x
Q

A
|
A

R
|
R

I
|
I

D
|
D

R
x
S

G
|
G

E
x
S

Q
x
E

V
|
V

I
|
I

V
|
V

G
|
G

V
|
V

N
|
N

R
x
K

Y
|
Y

R
x
Q

K
x
L

A
x
E

E
x
K

E
|
E

D
|
D

P
x
A

I
x
V

E
|
E

I
x
V

L
|
L

D
x
A

V
x
I

D
|
D

N
|
N

H
x
T

A
x
S

V
|
V

R
|
R

E
x
N

A
x
R

Q
|
Q

I
|
I

R
x
E

R
x
K

I
x
L

A
x
K

K
|
K

V
x
I

K
|
K

A
x
S

E
x
S

R
|
R

D
|
D

E
x
Q

A
|
A

A
x
L

C
x
A

Q
x
E

S
x
R

A
x
C

L
|
L

G
x
A

A
|
A

L
|
L

R
x
T

E
|
E

G
x
C

A
|
A

K
x
A

R
x
S

-
x
G

D
|
D

G
|
G

N
|
N

L
x
I

L
|
L

A
|
A

L
|
L

A
|
A

V
|
V

E
x
D

C
x
A

A
x
S

R
|
R

R
x
A

R
|
R

A
x
C

T
|
T

L
x
V

G
|
G

E
|
E

I
|
I

S
x
T

S
x
D

A
|
A

M
x
L

E
x
K

D
x
K

A
x
V

F
|
F

G
|
G

R
x
E

Y
x
H

G
x
K

T
x
A

N
|
N

P
x
D

A
x
R

P
x
M

V
|
V

S
|
S

G
|
G

V
x
A

Y
|
Y

G
x
R

G
x
Q

A
x
E

Y
x
F

T
x
G

G
x
E

D
x
S

A
x
K

R
x
E

W
x
I

T
|
T

G
x
S

L
x
A

V
x
I

D
x
K

G
x
R

V
|
V

E
x
H

M
x
K

V
x
F

G
x
M

K
x
E

R
|
R

M
x
E

G
|
G

R
|
R

K
x
R

P
|
P

R
|
R

L
|
L

V
|
V

A
|
A

K
|
K

M
|
M

G
|
G

Q
|
Q

D
|
D

G
|
G

H
|
H

D
|
D

R
|
R

G
|
G

A
|
A

N
x
K

L
x
V

V
x
I

S
x
A

S
x
T

M
x
G

F
|
F

G
x
A

D
|
D

L
|
L

G
|
G

F
|
F

E
x
D

V
|
V

V
x
D

P
x
I

G
|
G

P
|
P

L
|
L

F
|
F

Q
|
Q

T
|
T

P
|
P

K
x
R

E
|
E

A
x
V

A
|
A

A
x
Q

L
x
Q

A
|
A

V
|
V

E
x
D

R
x
A

Q
x
D

V
|
V

D
x
H

V
x
A

V
|
V

G
|
G

A
x
I

S
|
S

S
x
T

L
|
L

A
|
A

G
|
G

H
|
H

K
|
K

T
|
T

L
|
L

V
|
V

P
|
P

E
|
E

L
|
L

I
|
I

G
x
K

H
x
E

L
|
L

R
x
N

D
x
S

M
x
L

G
|
G

R
|
R

A
x
P

D
|
D

I
|
I

K
x
L

V
|
V

V
x
M

V
x
C

G
|
G

V
|
V

I
|
I

P
|
P

A
x
P

Q
|
Q

D
|
D

Y
|
Y

D
x
E

M
x
F

L
|
L

R
x
F

E
|
E

A
x
V

G
|
G

V
|
V

Q
x
S

G
x
N

I
x
V

F
|
F

G
|
G

P
|
P

G
|
G

T
|
T

N
x
R

L
x
I

I
x
P

D
x
K

A
|
A

G
x
V

E
x
Q

V
|
V

L
|
L

Q50 (≠ E18) binding malonyl-CoA
I69 (≠ V34) to V: in MMAM; likely benign; dbSNP:rs115923556
P86 (= P51) to L: in MMAM; mut0 and mut-; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs769348060
G87 (= G52) to E: in MMAM; mut0; dbSNP:rs1554160986
R93 (= R58) to H: in MMAM; mut0; decreased methylmalonyl-CoA mutase activity; dbSNP:rs121918251
G94 (= G59) to R: in MMAM; mut0; dbSNP:rs727504022; to V: in MMAM; mut- and mut0; dbSNP:rs535411418
P95 (≠ V60) to R: in MMAM; mut0; dbSNP:rs190834116
YPTM 96:99 (≠ RASM 61:64) binding malonyl-CoA
Y100 (= Y65) to C: in MMAM; mut-; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs864309735
W105 (= W70) to R: in MMAM; mut0; dbSNP:rs121918249
TIRQY 106:110 (= TIRQY 71:75) binding malonyl-CoA
R108 (= R73) to C: in MMAM; mut0; dbSNP:rs121918257; to G: in MMAM; mut-; to H: in MMAM; mut0; dbSNP:rs483352778
Q109 (= Q74) to R: in MMAM; mut0; dbSNP:rs1461110052
G133 (= G98) to R: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs879253828
A137 (= A102) to V: in MMAM; mut0; dbSNP:rs941483851
D139 (= D104) to N: in MMAM; uncertain significance; dbSNP:rs879253829
L140 (= L105) to P: in MMAM; decreased protein expression; decreased methylmalonyl-CoA mutase activity
A141 (= A106) to T: in MMAM; decreased protein expression; dbSNP:rs1554160730
H143 (= H108) to Y: in MMAM; mut0
G145 (= G110) to S: in MMAM; mut0
S148 (= S113) to L: in MMAM; mut0; dbSNP:rs1300547552
D156 (= D121) to N: in MMAM; mut-
G158 (= G123) to V: in MMAM; mut0
G161 (= G126) to R: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; to V: in MMAM; decreased protein expression
F174 (= F139) to S: in MMAM; mut0; dbSNP:rs864309733
M186 (= M151) to V: in MMAM; mut-; dbSNP:rs148331800
T187 (= T152) to S: in MMAM; mut0; dbSNP:rs879253830
N189 (= N154) to I: in MMAM; mut-; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs200908035; to K: in MMAM; mut-; dbSNP:rs1561959114
A191 (= A156) to E: in MMAM; mut- and mut0; affects proper folding; reduced protein level; decreased methylmalonyl-CoA mutase activity; dbSNP:rs760782399
A197 (= A162) to E: in MMAM; mut0
G203 (= G168) to R: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs778702777
E205 (= E170) natural variant: Missing (in MMAM; mut0; dbSNP:rs879253831)
G215 (= G180) to C: in MMAM; mut- and mut0; dbSNP:rs121918258; to S: in MMAM; mut0; dbSNP:rs121918258
TIQ 216:218 (= TIQ 181:183) binding malonyl-CoA
Q218 (= Q183) to H: in MMAM; mut0 and mut-; no effect on protein abundance; loss of methylmalonyl-CoA mutase activity; alters thermodynamic stability; dbSNP:rs1446389693
N219 (= N184) to Y: in MMAM; mut0; no effect on protein abundance; loss of methylmalonyl-CoA mutase activity; dbSNP:rs121918256
R228 (= R193) binding malonyl-CoA; to Q: in MMAM; mut0; dbSNP:rs770810987
T230 (= T195) to I: in MMAM; mut-; to R: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs879253833
Y231 (= Y196) to N: in MMAM; mut-; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity; strong decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs864309736
K255 (= K220) binding malonyl-CoA
S262 (= S227) to N: in MMAM; mut0
H265 (= H230) binding malonyl-CoA; to Y: in MMAM; mut-
E276 (= E241) to D: in MMAM; uncertain significance; mut-; dbSNP:rs12175488
L281 (≠ I246) to S: in MMAM; mut0; dbSNP:rs796052007
G284 (= G249) to E: in MMAM; mut0; dbSNP:rs879253835; to R: in MMAM; mut0; dbSNP:rs761477436
S288 (≠ V253) to P: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs1179778233
G291 (= G256) to E: in MMAM; mut0
Q293 (≠ A258) to P: in MMAM; mut0
RLS 304:306 (= RLS 269:271) binding malonyl-CoA
L305 (= L270) to S: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs1554160246
S306 (= S271) to F: in MMAM; mut0; dbSNP:rs1085307929
W309 (≠ F274) to G: in MMAM; decreased protein expression
G312 (= G277) to V: in MMAM; mut0; dbSNP:rs864309734
Y316 (≠ F281) to C: in MMAM; mut-; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity; no decreased affinity for adenosylcob(III)alamin; dbSNP:rs781474200
A324 (= A289) to T: in MMAM; mut-; dbSNP:rs780387525
R326 (= R291) to K: in MMAM; uncertain significance; dbSNP:rs758577372
L328 (= L293) to F: in MMAM; mut0; affects proper folding; no effect on protein abundance; loss of methylmalonyl-CoA mutase activity; dbSNP:rs796052002; to P: in MMAM; mut0; dbSNP:rs965316043
S344 (= S308) to F: in MMAM; mut-; affects proper folding; no effect on protein abundance; loss of methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin
L346 (≠ M310) natural variant: Missing (in MMAM; mut0)
L347 (= L311) to R: in MMAM; mut0; dbSNP:rs1026703654
H350 (= H314) to Y: in MMAM; mut0; dbSNP:rs1407914109
L358 (= L322) to P: in MMAM; mut0
N366 (= N330) to S: in MMAM; mut-; no effect on protein abundance; loss of methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs864309737
R369 (= R333) to C: in MMAM; mut0; dbSNP:rs772552898; to H: in MMAM; mut- and mut0; no effect on protein abundance; loss of methylmalonyl-CoA mutase activity; alters thermodynamic stability; dbSNP:rs564069299
T370 (= T334) to P: in MMAM; mut0; dbSNP:rs368790885
A377 (= A341) to E: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs121918250
Q383 (= Q347) to H: in MMAM; mut0; to P: in MMAM; mut0
H386 (= H350) to N: in MMAM; mut0; dbSNP:rs1554159937; to R: in MMAM; mut0; dbSNP:rs866933356
T387 (= T351) to I: in MMAM; mut-; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability
N388 (= N352) to H: in MMAM; mut0; dbSNP:rs766010704; to K: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs879253840
S389 (≠ A353) natural variant: Missing (in MMAM; mut0)
I412 (= I376) natural variant: Missing (in MMAM; mut0)
P424 (= P388) to L: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs879253842
G426 (= G390) to E: in MMAM; mut-; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs533755473; to R: in MMAM; mut-; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity; strong decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs769922244
G427 (= G391) to D: in MMAM; mut0; dbSNP:rs753288303
G454 (= G418) to E: in MMAM; mut0
A499 (≠ P463) to T: in dbSNP:rs2229385
I505 (≠ V469) to T: in MMAM; decreased protein expression; decreased methylmalonyl-CoA mutase activity
Q514 (= Q478) to E: in MMAM; uncertain significance; to K: in MMAM; decreased protein expression
L518 (≠ I482) to P: in MMAM; mut0; dbSNP:rs864309738
R532 (≠ S496) to H: in dbSNP:rs1141321
A535 (≠ G499) to P: in MMAM; mut0; dbSNP:rs760183775
A552 (= A515) to V: in MMAM; uncertain significance; dbSNP:rs879253845
C560 (≠ A523) to Y: in MMAM; mut0; dbSNP:rs1238333040
T566 (≠ S529) to R: in MMAM; mut0
F573 (= F536) to S: in MMAM; mut-; affects proper folding; no effect on protein abundance; no effect on methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; does not alter thermodynamic stability; dbSNP:rs775593146
Y587 (= Y550) to C: in MMAM; mut-
I597 (≠ W560) to R: in MMAM; no changed in protein expression; decreased methylmalonyl-CoA mutase activity; dbSNP:rs1554158951
P615 (= P578) to L: in MMAM; mut0; affects proper folding; reduced strongly protein level; to R: in MMAM; mut0; dbSNP:rs1554158777; to T: in MMAM; mut0; affects proper folding; reduced strongly protein level; loss of methylmalonyl-CoA mutase activity; dbSNP:rs1302409621
R616 (= R579) to C: in MMAM; mut0; dbSNP:rs765284825
L617 (= L580) to R: in MMAM; mut0; dbSNP:rs1554158775
K621 (= K584) to N: in MMAM; mut0
G623 (= G586) to R: in MMAM; mut0; dbSNP:rs121918254
Q624 (= Q587) to R: in MMAM; no effect on protein abundance; dbSNP:rs768521956
D625 (= D588) to G: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs879253847; to V: in MMAM; mut0; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity
G626 (= G589) to C: in MMAM; mut-; dbSNP:rs982110849
H627 (= H590) binding axial binding residue; to R: in MMAM; mut0; dbSNP:rs372486357
G630 (= G593) to E: in MMAM; mut0; dbSNP:rs143023066
V633 (≠ L596) to G: in MMAM; mut-; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; does not alter thermodynamic stability; dbSNP:rs200055428
G637 (≠ M600) to E: in MMAM; mut-; to R: in MMAM; mut0; dbSNP:rs781501004
F638 (= F601) to I: in MMAM; mut0
D640 (= D603) to Y: in MMAM; mut0; dbSNP:rs865815395
G642 (= G605) to R: in MMAM; mut-; dbSNP:rs747897332
G648 (= G611) to D: in MMAM; mut-; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity; strong decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs766721811
V669 (= V632) to E: in MMAM; mut0; dbSNP:rs1360470463
I671 (≠ A634) to V: in dbSNP:rs8589
L674 (= L637) to F: in MMAM; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs1164271240
H678 (= H641) to R: in MMAM; mut-; dbSNP:rs147094927
E684 (= E647) natural variant: E -> EL (in MMAM; mut-)
L685 (= L648) to R: in MMAM; mut-; dbSNP:rs864309739
R694 (= R657) to L: in MMAM; mut-; decreased protein abundance; loss of methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; to W: in MMAM; mut- and mut0; no effect on protein abundance; loss of methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs777758903
M700 (≠ V663) to K: in MMAM; mut-; no effect on protein abundance; loss of methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs140600746
G703 (= G666) to R: in MMAM; mut0; dbSNP:rs121918255
G717 (= G680) to V: in MMAM; mut-; no effect on protein abundance; interferes with the binding of the cofactor to the apoenzyme; decreased methylmalonyl-CoA mutase activity; strong decreased affinity for adenosylcob(III)alamin; decreased thermodynamic stability; dbSNP:rs121918252
G723 (= G686) to D: in MMAM; decreased protein expression; decreased methylmalonyl-CoA mutase activity; dbSNP:rs755077681

Sites not aligning to the query:

1:32 modified: transit peptide, Mitochondrion
7:750 natural variant: Missing (in MMAM; mut-)
152:750 natural variant: Missing (in MMAM; mut0)
228:750 natural variant: Missing (in MMAM; mut0)

2xiqA Crystal structure of human methylmalonyl-coa mutase in complex with adenosylcobalamin and malonyl-coa (see paper)
62% identity, 97% coverage: 4:699/714 of query aligns to 1:701/714 of 2xiqA

query
sites
2xiqA

K

Q

P

P

T

L

R

H

A

P

D

E

W

W

E

A

A

A

L

L

A

A

A

K

K

K

E

Q

V

L

K

K

G

G

R

K

-

N

-

P

-

E

D

D

L

L

T

I

W

W

H

H

T

T

P

P

E

E

G

G

I

I

A

S

V

I

K

K

P

P

L

L

Y

Y

T

S

A

K

E

R

D

D

V

T

A

M

D

D

I

L

D

P

P

E

G

E

L

L

P

P

G

G

F

V

A

K

P

P

F

F

T

T

R

R

G

G

V

P

R
x
Y

A

P

S
x
T

M
|
M

Y

Y

A

T

G

F

R
|
R

P

P

W

W

T
|
T

I

I

R
|
R

Q

Q

Y
|
Y

A

A

G

G

F

F

S

S

T

T

A

V

E

E

S

S

N

N

A

K

F

F

Y

Y

R

K

R

D

N

N

L

I

A

K

A

A

G

G

Q

Q

K

Q

G

G

L

L

S

S

V

V

A

A

F

F

D

D

L
|
L

A

A

T

T

H
|
H

R

R

G

G

Y

Y

D

D

S

S

D

D

H

N

P

P

R

R

V

V

T

R

G

G

D

D

V

V

G

G

K

M

A
|
A

G

G

V

V

A

A

I

I

D

D

S

T

V

V

E

E

D

D

M

T

K

K

I

I

L

L

F

F

D

D

G

G

I

I

P

P

L

L

D

E

K

K

M

M

S

S

V

V

S
|
S

M

M

T
|
T

M

M

N

N

G

G

A

A

V

V

I

I

P

P

I

V

L

L

A

A

F

N

F

F

I

I

V

V

A

T

G

G

E

E

Q

Q

G

G

V

V

A

P

Q

K

E

E

M

K

L

L

D

T

G

G

T
|
T

I

I

Q

Q

N

N

D

D

I

I

L

L

K

K

E

E

F

F

M

M

V

V

R
|
R

N

N

T

T

Y

Y

I

I

Y

F

P

P

E

E

P

P

S

S

M

M

R

K

I

I

S

A

D

D

I

I

F

F

A

E

F

Y

T

T

S

A

A

K

N

H

M

M

P

P

K
|
K

F

F

N

N

S

S

I

I

S

S

I

I

S

S

G

G

Y
|
Y

H
|
H

M

M

Q

Q

E
|
E

A

A

G

G

A

A

T

D

Q

A

V

I

Q

L

E

E

L

L

A

A

F

Y

T

T

I

L

A

A

D

D

G

G

M

L

E

E

Y

Y

V

S

K

R

Y

T

G

G

V

L

A

Q

S

A

G

G

L

L

D

T

I

I

D

D

R

E

F

F

A

A

G

P

R
|
R

L

L

S
|
S

F

F

F
|
F

F

W

A

G

I

I

G

G

M

M

N

N

F

F

F

Y

M

M

E

E

I

I

A

A

K

K

L

M

R

R

A

A

A

G

R

R

V

R

L

L

W

W

Y

A

R

H

V

L

M

I

T

E

S

K

L

M

-

F

G

Q

A

P

K

K

D

N

E

S

R

K

S

S

K

L

M

L

L

L

R
|
R

T
x
A

H
|
H

C

C

Q
|
Q

T

T

S

S

G
|
G

V
x
W

S

S

L

L

T

T

E

E

Q

Q

D

D

P

P

Y

Y

N

N

V

I

M

V

R

R

T

T

T

A

I

I

E

E

A

A

M

M

A

A

M

V

L

F

G

G

T

T

Q
|
Q

S

S

L

L

H

H

T

T

N

N

A

S

L

F

D

D

E
|
E

A

A

I

L

A
x
G

L
|
L

P

P

T

T

D

V

F

K

S

S

A

A

R

R

I

I

A

A

R

R

N

N

T

T

Q

Q

L

I

V

I

I

I

Q

Q

E

E

T

S

G

G

M

I

T

P

N

K

V

V

V

A

D

D

P

P

L

W

G

G

S

S

Y

Y

Y

M

V

M

E

E

A

C

L

L

T

T

Q

N

R

D

L

V

V

Y

D

D

E

A

A

A

W

L

A

K

I

L

I

I

E

N

R

E

V

I

E

E

A

E

E

M

G

G

M

M

A

A

K

K

A

A

V

V

A

A

A

E

G

G

W

I

P

P

K

K

A

L

M

R

I

I

E

E

A

C

A

A

A

R

A

Q

A

A

R

R

I

I

D

D

R

S

G

G

E

S

Q

E

V

V

I

I

V

V

G

G

V

V

N

N

R

K

Y

Y

R

Q

K

L

A

E

E

K

E

E

D

D

P

T

I

V

E

E

I

V

L

L

D

A

V

I

D

D

N

N

H

T

A

S

V

V

R

R

E

N

A

R

Q

Q

I

I

R

E

R

K

I

L

A

K

K

K

V

I

K

K

A

S

E

S

R

R

D

D

E

Q

A

A

A

L

C

A

Q

E

S

R

A

C

L

L

G

A

A

A

L

L

R

T

E

E

G

C

A

A

K

A

R

S

-

G

D

D

G

G

N

N

L

I

L

L

A

A

L

L

A

A

V

V

E

D

C

A

A

S

R

R

R

A

R

R

A

C

T

T

L

V

G

G

E

E

I

I

S

T

S

D

A

A

M

L

E

K

D

K

A

V

F

F

G

G

R

E

Y

H

G

K

T

A

N

N

P

D

A

R

P

M

V

V

S

S

G

G

V

A

Y

Y

G

R

G

Q

A

E

Y

F

T

G

G

E

D

S

A

K

R

E

W

I

T

T

G

S

L

A

V

I

D

K

G

R

V

V

E

H

M

K

V

F

G

M

K

E

R

R

M

E

G

G

R

R

K

R

P

P

R

R

L

L

V

V

A

A

K
|
K

M

M

G

G

Q

Q

D
|
D

G
|
G

H
|
H

D
|
D

R
|
R

G
|
G

A

A

N

K

L

V

V
x
I

S

A

S

T

M

G

F

F

G

A

D

D

L

L

G

G

F

F

E

D

V

V

V

D

P

I

G

G

P

P

L

L

F

F

Q

Q

T

T

P

P

K

R

E

E

A

V

A

A

A

Q

L

Q

A

A

V

V

E

D

R

A

Q

D

V

V

D

H

V

A

V

V

G
|
G

A

V

S
|
S

S

T

L
|
L

A

A

A
|
A

G

G

H

H

K

K

T

T

L

L

V

V

P

P

E

E

L

L

I

I

G

K

H

E

L

L

R

N

D

S

M

L

G

G

R

R

A

P

D

D

I

I

K

L

V

V

V

M

V

C

G
|
G

V

V

I

I

P

P

A

P

Q

Q

D

D

Y

Y

D

E

M

F

L

L

R

F

E

E

A

V

G

G

V

V

Q

S

G

N

I

V

F
|
F

G
|
G

P

P

G

G

T
|
T

N

R

L

I

I

P

D

K

A

A

G

V

E

Q

V

V

L

L

active site: Y75 (= Y75), Y229 (= Y229), H230 (= H230), K586 (= K584), D590 (= D588), H592 (= H590)
binding cobalamin: Y75 (= Y75), L105 (= L105), H108 (= H108), A125 (= A125), R193 (= R193), E233 (= E233), G320 (= G319), W321 (≠ V320), E357 (= E356), G360 (≠ A359), L361 (= L360), G591 (= G589), H592 (= H590), D593 (= D591), R594 (= R592), G595 (= G593), I599 (≠ V597), G635 (= G633), S637 (= S635), L639 (= L637), A641 (= A639), G667 (= G665), G668 (= G666), F687 (= F685), G688 (= G686), T691 (= T689)
binding malonyl-coenzyme a: Y61 (≠ R61), T63 (≠ S63), M64 (= M64), R68 (= R68), T71 (= T71), R73 (= R73), Y75 (= Y75), S150 (= S150), T152 (= T152), T181 (= T181), R193 (= R193), K220 (= K220), H230 (= H230), R269 (= R269), S271 (= S271), F273 (= F273), R313 (= R312), A314 (≠ T313), H315 (= H314), Q317 (= Q316), Q348 (= Q347)

8dyjB Crystal structure of human methylmalonyl-coa mutase in complex with adp and cob(ii)alamin (see paper)
62% identity, 97% coverage: 5:699/714 of query aligns to 1:700/708 of 8dyjB

query
sites
8dyjB

K

Q

P

P

T

L

R

H

A

P

D

E

W

W

E

A

A

A

L

L

A

A

A

K

K

K

E

Q

V

L

K

K

G

G

R

K

-

N

-

P

-

E

D

D

L

L

T

I

W

W

H

H

T

T

P

P

E

E

G

G

I

I

A

S

V

I

K

K

P

P

L

L

Y

Y

T

S

A

K

E

R

D

D

V

T

A

M

D

D

I

L

D

P

P

E

G

E

L

L

P

P

G

G

F

V

A

K

P

P

F

F

T

T

R

R

G

G

V

P

R

Y

A

P

S

T

M

M

Y

Y

A

T

G

F

R

R

P

P

W

W

T

T

I

I

R

R

Q

Q

Y
|
Y

A

A

G

G

F

F

S

S

T

T

A

V

E

E

S

S

N

N

A

K

F

F

Y

Y

R

K

R

D

N

N

L

I

A

K

A

A

G

G

Q

Q

K

Q

G

G

L

L

S

S

V

V

A

A

F
|
F

D

D

L
|
L

A

A

T

T

H
|
H

R

R

G

G

Y

Y

D

D

S

S

D

D

H

N

P

P

R

R

V

V

T

R

G

G

D

D

V

V

G

G

K

M

A
|
A

G

G

V

V

A

A

I

I

D

D

S

T

V

V

E

E

D

D

M

T

K

K

I

I

L

L

F

F

D

D

G

G

I

I

P

P

L

L

D

E

K

K

M

M

S

S

V

V

S

S

M

M

T
|
T

M

M

N

N

G

G

A

A

V

V

I

I

P

P

I

V

L

L

A

A

F

N

F

F

I

I

V

V

A

T

G

G

E

E

Q

Q

G

G

V

V

A

P

Q

K

E

E

M

K

L

L

D

T

G

G

T

T

I

I

Q

Q

N

N

D

D

I

I

L

L

K

K

E

E

F

F

M

M

V
|
V

R
|
R

N

N

T

T

Y

Y

I

I

Y

F

P

P

E

E

P

P

S

S

M

M

R

K

I

I

S

A

D

D

I

I

F

F

A

E

F

Y

T

T

S

A

A

K

N

H

M

M

P

P

K

K

F

F

N

N

S

S

I

I

S

S

I

I

S

S

G

G

Y
|
Y

H
|
H

M

M

Q

Q

E
|
E

A

A

G

G

A

A

T

D

Q

A

V

I

Q

L

E

E

L

L

A

A

F

Y

T

T

I

L

A

A

D

D

G

G

M

L

E

E

Y

Y

V

S

K

R

Y

T

G

G

V

L

A

Q

S

A

G

G

L

L

D

T

I

I

D

D

R

E

F

F

A

A

G

P

R

R

L

L

S

S

F

F

F
|
F

F

W

A

G

I

I

G

G

M

M

N

N

F

F

F

Y

M

M

E

E

I

I

A

A

K

K

L

M

R

R

A

A

A

G

R

R

V

R

L

L

W

W

Y

A

R

H

V

L

M

I

T

E

S

K

L

M

-

F

G

Q

A

P

K

K

D

N

E

S

R

K

S

S

K

L

M

L

L

L

R

R

T

A

H

H

C

C

Q
|
Q

T

T

S

S

G
|
G

V
x
W

S

S

L

L

T

T

E

E

Q

Q

D

D

P

P

Y

Y

N

N

V

I

M

V

R

R

T

T

T

A

I

I

E

E

A

A

M

M

A

A

M

V

L

F

G

G

T

T

Q

Q

S

S

L

L

H

H

T

T

N
|
N

A

S

L

F

D

D

E
|
E

A

A

I

L

A
x
G

L
|
L

P
|
P

T

T

D

V

F

K

S

S

A

A

R

R

I

I

A

A

R

R

N

N

T

T

Q

Q

L

I

V

I

I

I

Q

Q

E

E

T

S

G

G

M

I

T

P

N

K

V

V

V

A

D

D

P

P

L

W

G

G

S

S

Y

Y

Y

M

V

M

E

E

A

C

L

L

T

T

Q

N

R

D

L

V

V

Y

D

D

E

A

A

A

W

L

A

K

I

L

I

I

E

N

R

E

V

I

E

E

A

E

E

M

G

G

M

M

A

A

K

K

A

A

V

V

A

A

A

E

G

G

W

I

P

P

K

K

A

L

M

R

I

I

E

E

A

C

A

A

A

R

A

Q

A

A

R

R

I

I

D

D

R

S

G

G

E

S

Q

E

V

V

I

I

V

V

G

G

V

V

N

N

R

K

Y

Y

R

Q

K

L

A

E

E

K

E

E

D

D

P

T

I

V

E

E

I

V

L

L

D

A

V

I

D

D

N

N

H

T

A

S

V

V

R

R

E

N

A

R

Q

Q

I

I

R

E

R

K

I

L

A

K

K

K

V

I

K

K

A

S

E

S

R

R

D

D

E

Q

A

A

A

L

C

A

Q

E

S

R

A

C

L

L

G

A

A

A

L

L

R

T

E

E

G

C

A

A

K

A

R

S

-

G

D

D

G

G

N

N

L

I

L

L

A

A

L

L

A

A

V

V

E

D

C

A

A

S

R

R

R

A

R

R

A

C

T

T

L

V

G

G

E

E

I

I

S

T

S

D

A

A

M

L

E

K

D

K

A

V

F

F

G

G

R

E

Y

H

G

K

T

A

N

N

P

D

A

R

P

M

V

V

S

S

G

G

V

A

Y

Y

G

R

G

Q

A

E

Y

F

T

G

G

E

D

S

A

K

R

E

W

I

T

T

G

S

L

A

V

I

D

K

G

R

V

V

E

H

M

K

V

F

G

M

K

E

R

R

M

E

G

G

R

R

K

R

P

P

R

R

L

L

V

V

A

A

K

K

M

M

G

G

Q

Q

D

D

G
|
G

H
|
H

D
|
D

R
|
R

G
|
G

A

A

N

K

L

V

V
x
I

S

A

S

T

M

G

F

F

G

A

D

D

L

L

G

G

F

F

E

D

V

V

V

D

P

I

G

G

P

P

L

L

F

F

Q

Q

T

T

P

P

K

R

E

E

A

V

A

A

A

Q

L

Q

A

A

V

V

E

D

R

A

Q

D

V

V

D

H

V

A

V

V

G

G

A

V

S
|
S

S

T

L
|
L

A

A

A
|
A

G

G

H

H

K

K

T

T

L

L

V

V

P

P

E

E

L

L

I

I

G

K

H

E

L

L

R

N

D

S

M

L

G

G

R

R

A

P

D

D

I

I

K

L

V

V

V

M

V

C

G
|
G

V
|
V

I

I

P

P

A

P

Q

Q

D

D

Y

Y

D

E

M

F

L

L

R

F

E

E

A

V

G

G

V

V

Q

S

G

N

I

V

F
|
F

G
|
G

P

P

G

G

T
|
T

N

R

L

I

I

P

D

K

A

A

G

V

E

Q

V

V

L

L

binding adenosine-5'-diphosphate: Y74 (= Y75), T151 (= T152), R192 (= R193), Y228 (= Y229), H229 (= H230), F272 (= F273), Q316 (= Q316), N352 (= N352), E356 (= E356), L360 (= L360), P361 (= P361)
binding cobalamin: F102 (= F103), L104 (= L105), H107 (= H108), A124 (= A125), V191 (= V192), R192 (= R193), H229 (= H230), E232 (= E233), G319 (= G319), W320 (≠ V320), E356 (= E356), G359 (≠ A359), L360 (= L360), G590 (= G589), H591 (= H590), D592 (= D591), R593 (= R592), G594 (= G593), I598 (≠ V597), S636 (= S635), L638 (= L637), A640 (= A639), G666 (= G665), G667 (= G666), V668 (= V667), F686 (= F685), G687 (= G686), T690 (= T689)

8gjuJ Crystal structure of human methylmalonyl-coa mutase (mmut) in complex with methylmalonic acidemia type a protein (mmaa), coenzyme a, and gdp (see paper)
60% identity, 97% coverage: 4:699/714 of query aligns to 1:678/689 of 8gjuJ

query
sites
8gjuJ

K

Q

P

P

T

L

R

H

A

P

D

E

W

W

E

A

A

A

L

L

A

A

A

K

K

K

E

Q

V

L

K

K

G

G

R

K

-

N

-

P

-

E

D

D

L

L

T

I

W

W

H

H

T

T

P

P

E

E

G

G

I

I

A

S

V

I

K

K

P

P

L

L

Y

Y

T

S

A

K

E

R

D

D

V

T

A

M

D

D

I

L

D

P

P

E

G

E

L

L

P

P

G

G

F

V

A

K

P

P

F

F

T

T

R

R

G

G

V

P

R
x
Y

A

P

S
x
T

M

M

Y

Y

A

T

G

F

R
|
R

P

P

W

W

T
|
T

I

I

R
|
R

Q

Q

Y

Y

A

A

G

G

F

F

S

S

T

T

A

V

E

E

S

S

N

N

A

K

F

F

Y

Y

R

K

R

D

N

N

L

I

A

K

A

A

G

G

Q

Q

K

Q

G

G

L

L

S

S

V

V

A

A

F

F

D

D

L

L

A

A

T

T

H

H

R

R

G

G

Y

Y

D

D

S

S

D

D

H

N

P

P

R

R

V

V

T

R

G

G

D

D

V

V

G

G

K

M

A

A

G

G

V

V

A

A

I

I

D

D

S

T

V

V

E

E

D

D

M

T

K

K

I

I

L

L

F

F

D

D

G

G

I

I

P

P

L

L

D

E

K

K

M

M

S

S

V

V

S
|
S

M

M

T
|
T

M

M

N

N

G

G

A

A

V

V

I

I

P

P

I

V

L

L

A

A

F

N

F

F

I
<