SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_085218187.1 NCBI__GCF_900177405.1:WP_085218187.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P18669 Phosphoglycerate mutase 1; BPG-dependent PGAM 1; Phosphoglycerate mutase isozyme B; PGAM-B; EC 5.4.2.11; EC 5.4.2.4 from Homo sapiens (Human) (see 4 papers)
62% identity, 94% coverage: 4:217/228 of query aligns to 6:224/254 of P18669

query
sites
P18669

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10:17 (vs. 8:15, 88% identical) binding substrate
H11 (= H9) active site, Tele-phosphohistidine intermediate
SG 23:24 (≠ TG 21:22) binding substrate
Y26 (≠ W24) modified: Phosphotyrosine
ERHY 89:92 (= ERHY 87:90) binding substrate
K100 (= K98) binding substrate
RR 116:117 (= RR 114:115) binding substrate

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
251 modified: N6-acetyllysine; alternate
253 modified: N6-acetyllysine
254 modified: N6-acetyllysine

Q3JWH7 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase; BPG-dependent PGAM; PGAM; Phosphoglyceromutase; dPGM; EC 5.4.2.11 from Burkholderia pseudomallei (strain 1710b) (see paper)
58% identity, 100% coverage: 1:228/228 of query aligns to 1:231/249 of Q3JWH7

query
sites
Q3JWH7

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R8 (= R8) binding (2R)-2,3-bisphosphoglycerate
H9 (= H9) active site, Tele-phosphohistidine intermediate; binding (2R)-2,3-bisphosphoglycerate
N15 (= N15) binding (2R)-2,3-bisphosphoglycerate
T21 (= T21) binding (2R)-2,3-bisphosphoglycerate; binding (2R)-3-phosphoglycerate
G22 (= G22) binding (2R)-2,3-bisphosphoglycerate; binding (2R)-3-phosphoglycerate
R60 (= R60) binding (2R)-2,3-bisphosphoglycerate
E87 (= E87) binding (2R)-2,3-bisphosphoglycerate; binding (2R)-3-phosphoglycerate
Y90 (= Y90) binding (2R)-2,3-bisphosphoglycerate; binding (2R)-3-phosphoglycerate
K98 (= K98) binding (2R)-2,3-bisphosphoglycerate; binding (2R)-3-phosphoglycerate
R114 (= R114) binding (2R)-2,3-bisphosphoglycerate; binding (2R)-3-phosphoglycerate
R115 (= R115) binding (2R)-2,3-bisphosphoglycerate; binding (2R)-3-phosphoglycerate
H182 (= H179) binding (2R)-2,3-bisphosphoglycerate
G183 (= G180) binding (2R)-2,3-bisphosphoglycerate
N184 (= N181) binding (2R)-2,3-bisphosphoglycerate; binding (2R)-3-phosphoglycerate

7xb8B Phosphoglycerate mutase 1 complexed with a covalent inhibitor
62% identity, 94% coverage: 4:217/228 of query aligns to 4:222/249 of 7xb8B

query
sites
7xb8B

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L

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binding 4-(cyclopenten-1-yl)-2-[(3-sulfophenyl)sulfonylamino]benzoic acid: F20 (= F20), R88 (= R88), Y90 (= Y90), L93 (= L93), K98 (= K98), V110 (= V110), W113 (= W113), R114 (= R114), N186 (= N181)

7xb7B Phosphoglycerate mutase 1 complexed with a covalent inhibitor
62% identity, 94% coverage: 4:217/228 of query aligns to 4:222/246 of 7xb7B

query
sites
7xb7B

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L

binding 3-[[5-(cyclopenten-1-yl)-2-(methylcarbamoyl)phenyl]sulfamoyl]benzenesulfonic acid: F20 (= F20), R88 (= R88), Y90 (= Y90), L93 (= L93), K98 (= K98), V110 (= V110), R114 (= R114), N186 (= N181)

3gp5A Crystal structure of phosphoglyceromutase from burkholderia pseudomallei with 3-phosphoglyceric acid and vanadate (see paper)
58% identity, 100% coverage: 1:228/228 of query aligns to 1:231/248 of 3gp5A

query
sites
3gp5A

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N

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active site: H9 (= H9), R60 (= R60), E87 (= E87), H182 (= H179)
binding vanadate ion: R8 (= R8), H9 (= H9), N15 (= N15), R60 (= R60), E87 (= E87), H182 (= H179), G183 (= G180)

1yfkA Crystal structure of human b type phosphoglycerate mutase (see paper)
62% identity, 94% coverage: 4:217/228 of query aligns to 4:222/243 of 1yfkA

query
sites
1yfkA

L

L

V

V

L

L

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G

Q

E

S

S

A

A

W

W

N

N

L

L

E

E

N

N

R

R

F

F

T

S

G

G

W

W

W

Y

D

D

V

A

N

D

L

L

T

S

P

P

Q

A

G

G

E

H

A

E

E

E

A

A

K

K

A

R

A

G

G

G

E

Q

L

A

L

L

A

R

E

D

R

A

G

G

L

Y

D

E

F

F

D

D

I

I

C

C

F

F

T

T

S

S

L

V

Q

Q

T

K

R
|
R

A

A

I

I

K

R

T

T

L

L

N

W

I

T

A

V

L

L

E

D

A

A

M

I

G

D

R

Q

L

M

W

W

L

L

P

P

V
|
V

E

V

K
x
R

H

T

W

W

R

R

L

L

N

N

E
|
E

R

R

H

H

Y

Y

G

G

L

L

T

T

G

G

L

L

D

N

K

K

A

A

E

E

T

T

A

A

K

K

H

H

S

G

A

E

E

A

Q

Q

V

V

H

K

I

I

W

W

R

R

S

S

F

Y

D

D

I

V

P

P

A

P

M

M

E

E

P

P

G

D

G

H

D

P

F

F

-

Y

-

S

D

N

L

I

T

S

N

K

D

D

R

R

Y

Y

A

A

G

D

I

L

-

T

-

E

-

D

E

Q

I

L

P

P

S

S

H

C

E

E

S

S

L

L

K

K

D

D

T

T

I

I

A

A

R

R

V

A

L

L

P

P

Y

F

W

W

E

N

E

E

R

E

I

I

I

V

P

P

E

Q

L

I

K

K

E

E

G

G

R

K

R

R

V

V

L

L

V

I

S

A

A

A

H
|
H

G

G

N

N

S

S

L

L

R

R

A

G

L

I

V

V

K

K

H

H

L

L

N

E

R

G

I

L

T

S

D

E

A

E

D

A

I

I

S

M

G

E

L

L

E

N

I

L

P

P

T

T

G

G

Q

I

P

P

I

I

V

V

F

Y

E

E

L

L

D

D

D

K

S

N

L

L

active site: H9 (= H9), R60 (= R60), E87 (= E87), H184 (= H179)
binding chloride ion: V79 (= V79), R81 (≠ K81)

8it8C Phosphoglycerate mutase 1 complexed with a compound
62% identity, 94% coverage: 4:217/228 of query aligns to 5:223/240 of 8it8C

query
sites
8it8C

L

L

V

V

L

L

I

I

R

R

H

H

G

G

Q

E

S

S

A

A

W

W

N
|
N

L

L

E

E

N
|
N

R
|
R

F
|
F

T

S

G

G

W

W

W

Y

D

D

V

A

N

D

L

L

T

S

P

P

Q

A

G

G

E

H

A

E

E

E

A

A

K

K

A

R

A

G

G

G

E

Q

L

A

L

L

A

R

E

D

R

A

G

G

L

Y

D

E

F

F

D

D

I

I

C

C

F

F

T

T

S

S

L

V

Q

Q

T

K

R

R

A

A

I

I

K

R

T

T

L

L

N

W

I

T

A

V

L

L

E

D

A

A

M

I

G

D

R

Q

L

M

W

W

L

L

P

P

V

V

E

V

K

R

H

T

W

W

R

R

L

L

N

N

E

E

R
|
R

H

H

Y

Y

G

G

L

L

T

T

G

G

L

L

D

N

K
|
K

A

A

E

E

T

T

A

A

K

K

H

H

S

G

A

E

E

A

Q

Q

V

V

H

K

I

I

W

W

R
|
R

R

R

S

S

F

Y

D

D

I

V

P

P

A

P

M

M

E

E

P

P

G

D

G

H

D

P

F

F

-

Y

-

S

D

N

L

I

T

S

N

K

D

D

R

R

Y

Y

A

A

G

D

I

L

-

T

-

E

-

D

E

Q

I

L

P

P

S

S

H

C

E

E

S

S

L

L

K

K

D

D

T

T

I

I

A

A

R

R

V

A

L

L

P

P

Y

F

W

W

E

N

E

E

R

E

I

I

I

V

P

P

E

Q

L

I

K

K

E

E

G

G

R

K

R

R

V

V

L

L

V

I

S

A

A

A

H

H

G

G

N

N

S

S

L

L

R

R

A

G

L

I

V

V

K

K

H

H

L

L

N

E

R

G

I

L

T

S

D

E

A

E

D

A

I

I

S

M

G

E

L

L

E

N

I

L

P

P

T

T

G

G

Q

I

P

P

I

I

V

V

F

Y

E

E

L

L

D

D

D

K

S

N

L

L

binding 4-(4-chlorophenyl)-2-[(4-phenylphenyl)sulfonylamino]benzoic acid: N16 (= N15), N19 (= N18), R20 (= R19), F21 (= F20), R89 (= R88), K99 (= K98), R115 (= R114)

5zs8C Acetylation of lysine 100 of phosphoglycerate mutase 1 complexed with kh_ol
62% identity, 94% coverage: 4:217/228 of query aligns to 5:223/240 of 5zs8C

query
sites
5zs8C

L

L

V

V

L

L

I

I

R

R

H

H

G

G

Q

E

S

S

A

A

W

W

N

N

L

L

E

E

N
|
N

R

R

F
|
F

T

S

G

G

W

W

W

Y

D

D

V

A

N

D

L

L

T

S

P

P

Q

A

G

G

E

H

A

E

E

E

A

A

K

K

A

R

A

G

G

G

E

Q

L

A

L

L

A

R

E

D

R

A

G

G

L

Y

D

E

F

F

D

D

I

I

C

C

F

F

T

T

S

S

L

V

Q

Q

T

K

R

R

A

A

I

I

K

R

T

T

L

L

N

W

I

T

A

V

L

L

E

D

A

A

M

I

G

D

R

Q

L

M

W

W

L

L

P

P

V

V

E

V

K

R

H

T

W

W

R

R

L

L

N

N

E

E

R
|
R

H

H

Y

Y

G

G

L

L

T

T

G

G

L

L

D

N

K
|
K

A

A

E

E

T

T

A

A

K

K

H

H

S

G

A

E

E

A

Q

Q

V

V

H

K

I

I

W

W

R
|
R

R

R

S

S

F

Y

D

D

I

V

P

P

A

P

M

M

E

E

P

P

G

D

G

H

D

P

F

F

-

Y

-

S

D

N

L

I

T

S

N

K

D

D

R

R

Y

Y

A

A

G

D

I

L

-

T

-

E

-

D

E

Q

I

L

P

P

S

S

H

C

E

E

S

S

L

L

K

K

D

D

T

T

I

I

A

A

R

R

V

A

L

L

P

P

Y

F

W

W

E

N

E

E

R

E

I

I

I

V

P

P

E

Q

L

I

K

K

E

E

G

G

R

K

R

R

V

V

L

L

V

I

S

A

A

A

H

H

G

G

N

N

S

S

L

L

R

R

A

G

L

I

V

V

K

K

H

H

L

L

N

E

R

G

I

L

T

S

D

E

A

E

D

A

I

I

S

M

G

E

L

L

E

N

I

L

P

P

T

T

G

G

Q

I

P

P

I

I

V

V

F

Y

E

E

L

L

D

D

D

K

S

N

L

L

binding N-(3,4-dihydroxy-9,10-dioxo-9,10-dihydroanthracen-2-yl)-2-hydroxybenzene-1-sulfonamide: N19 (= N18), F21 (= F20), R89 (= R88), K99 (= K98), R115 (= R114)

3fdzA Crystal structure of phosphoglyceromutase from burkholderia pseudomallei 1710b with bound 2,3-diphosphoglyceric acid and 3- phosphoglyceric acid (see paper)
58% identity, 100% coverage: 1:227/228 of query aligns to 1:230/230 of 3fdzA

query
sites
3fdzA

M

M

P

Y

T

K

L

L

V

V

L

L

I

I

R
|
R

H
|
H

G

G

Q

E

S

S

A

T

W

W

N
|
N

L

K

E

E

N

N

R

R

F

F

T
|
T

G

G

W

W

W

V

D

D

V

V

N

D

L

L

T

T

P

E

Q

Q

G

G

E

N

A

R

E

E

A

A

K

R

A

Q

A

A

G

G

E

Q

L

L

A

K

E

E

R

A

G

G

L

Y

D

T

F

F

D

D

I

I

C

A

F

Y

T

T

S

S

L

V

Q

L

T

K

R
|
R

A

A

I

I

K

R

T

T

L

L

N

W

I

H

A

V

L

Q

E

D

A

Q

M

M

G

D

R

L

L

M

W

Y

L

V

P

P

V

V

E

V

K

H

H

S

W

W

R

R

L

L

N

N

E
|
E

R

R

H

H

Y
|
Y

G

G

G

A

L

L

T

S

G

G

L

L

D

N

K
|
K

A

A

E

E

T

T

A

A

K

K

H

Y

S

G

A

D

E

E

Q

Q

V

V

H

L

I

V

W

W

R
|
R

S

S

F

Y

D

D

I

T

P

P

A

A

M

L

E

E

P

P

G

G

G

D

D

E

F

R

D

A

L

P

T

Y

N

A

D

D

R

P

R

R

Y

Y

A

A

G

K

I

V

-

P

-

R

-

E

E

Q

I

L

P

P

S

L

H

T

E

E

S

C

L

L

K

K

D

D

T

T

I

V

A

A

R

R

V

V

L

L

P

P

Y

L

W

W

E

N

E

E

R

S

I

I

I

A

P

P

E

A

L

V

K

K

E

A

G

G

R

K

R

Q

V

V

L

L

V

I

S

A

A

A

H
|
H

G
|
G

N
|
N

S

S

L

L

R

R

A

A

L

L

V

I

K

K

H

Y

L

L

N

D

R

G

I

I

T

S

D

D

A

A

D

D

I

I

S

V

G

G

L

L

E

N

I

I

P

P

T

N

G

G

Q

V

P

P

I

L

V

V

F

Y

E

E

L

L

D

D

D

E

S

S

L

L

D

T

A

P

K

I

D

R

F

H

Y

Y

L

L

K

G

D

D

active site: H9 (= H9), R60 (= R60), E87 (= E87), H182 (= H179)
binding (2R)-2,3-diphosphoglyceric acid: R8 (= R8), H9 (= H9), N15 (= N15), T21 (= T21), R60 (= R60), E87 (= E87), Y90 (= Y90), K98 (= K98), R114 (= R114), R115 (= R115), H182 (= H179), G183 (= G180), N184 (= N181)

5y65C Phosphoglycerate mutase 1 complexed with a small molecule inhibitor kh2
62% identity, 94% coverage: 4:217/228 of query aligns to 4:222/239 of 5y65C

query
sites
5y65C

L

L

V

V

L

L

I

I

R

R

H
|
H

G

G

Q

E

S

S

A

A

W

W

N

N

L

L

E

E

N
|
N

R
|
R

F
|
F

T

S

G

G

W

W

W

Y

D

D

V

A

N

D

L

L

T

S

P

P

Q

A

G

G

E

H

A

E

E

E

A

A

K

K

A

R

A

G

G

G

E

Q

L

A

L

L

A

R

E

D

R

A

G

G

L

Y

D

E

F

F

D

D

I

I

C

C

F

F

T

T

S

S

L

V

Q

Q

T

K

R
|
R

A

A

I

I

K

R

T

T

L

L

N

W

I

T

A

V

L

L

E

D

A

A

M

I

G

D

R

Q

L

M

W

W

L

L

P

P

V

V

E

V

K

R

H

T

W

W

R

R

L

L

N

N

E
|
E

R
|
R

H

H

Y

Y

G

G

L

L

T

T

G

G

L

L

D

N

K
|
K

A

A

E

E

T

T

A

A

K

K

H

H

S

G

A

E

E

A

Q

Q

V

V

H

K

I

I

W
|
W

R
|
R

R

R

S

S

F

Y

D

D

I

V

P

P

A

P

M

M

E

E

P

P

G

D

G

H

D

P

F

F

-

Y

-

S

D

N

L

I

T

S

N

K

D

D

R

R

Y

Y

A

A

G

D

I

L

-

T

-

E

-

D

E

Q

I

L

P

P

S

S

H

C

E

E

S

S

L

L

K

K

D

D

T

T

I

I

A

A

R

R

V

A

L

L

P

P

Y

F

W

W

E

N

E

E

R

E

I

I

I

V

P

P

E

Q

L

I

K

K

E

E

G

G

R

K

R

R

V

V

L

L

V

I

S

A

A

A

H
|
H

G

G

N

N

S

S

L

L

R

R

A

G

L

I

V

V

K

K

H

H

L

L

N

E

R

G

I

L

T

S

D

E

A

E

D

A

I

I

S

M

G

E

L

L

E

N

I

L

P

P

T

T

G

G

Q

I

P

P

I

I

V

V

F

Y

E

E

L

L

D

D

D

K

S

N

L

L

active site: H9 (= H9), R60 (= R60), E87 (= E87), H184 (= H179)
binding ~{N}-[3,4-bis(oxidanyl)-9,10-bis(oxidanylidene)anthracen-2-yl]-4-(dimethylamino)benzenesulfonamide: N18 (= N18), R19 (= R19), F20 (= F20), R88 (= R88), K98 (= K98), W113 (= W113), R114 (= R114)

5y2uB X-ray structure of phosphoglycerate mutase 1(pgam1) complexed with a small molecule
62% identity, 94% coverage: 4:217/228 of query aligns to 4:222/237 of 5y2uB

query
sites
5y2uB

L

L

V

V

L

L

I

I

R

R

H
|
H

G

G

Q

E

S

S

A

A

W

W

N

N

L

L

E

E

N

N

R

R

F
|
F

T

S

G

G

W

W

W

Y

D

D

V

A

N

D

L

L

T

S

P

P

Q

A

G

G

E

H

A

E

E

E

A

A

K

K

A

R

A

G

G

G

E

Q

L

A

L

L

A

R

E

D

R

A

G

G

L

Y

D

E

F

F

D

D

I

I

C

C

F

F

T

T

S

S

L

V

Q

Q

T

K

R
|
R

A

A

I

I

K

R

T

T

L

L

N

W

I

T

A

V

L

L

E

D

A

A

M

I

G

D

R

Q

L

M

W

W

L

L

P

P

V

V

E

V

K

R

H

T

W

W

R

R

L

L

N

N

E
|
E

R
|
R

H

H

Y

Y

G

G

L
|
L

T

T

G

G

L

L

D

N

K

K

A

A

E

E

T

T

A

A

K

K

H

H

S

G

A

E

E

A

Q

Q

V

V

H

K

I

I

W
|
W

R
|
R

R

R

S

S

F

Y

D

D

I

V

P

P

A

P

M

M

E

E

P

P

G

D

G

H

D

P

F

F

-

Y

-

S

D

N

L

I

T

S

N

K

D

D

R

R

Y

Y

A

A

G

D

I

L

-

T

-

E

-

D

E

Q

I

L

P

P

S

S

H

C

E

E

S

S

L

L

K

K

D

D

T

T

I

I

A

A

R

R

V

A

L

L

P

P

Y

F

W

W

E

N

E

E

R

E

I

I

I

V

P

P

E

Q

L

I

K

K

E

E

G

G

R

K

R

R

V

V

L

L

V

I

S

A

A

A

H
|
H

G

G

N

N

S

S

L

L

R

R

A

G

L

I

V

V

K

K

H

H

L

L

N

E

R

G

I

L

T

S

D

E

A

E

D

A

I

I

S

M

G

E

L

L

E

N

I

L

P

P

T

T

G

G

Q

I

P

P

I

I

V

V

F

Y

E

E

L

L

D

D

D

K

S

N

L

L

active site: H9 (= H9), R60 (= R60), E87 (= E87), H184 (= H179)
binding alizarin red: F20 (= F20), R88 (= R88), L93 (= L93), W113 (= W113), R114 (= R114)

8itdC Phosphoglycerate mutase 1 complexed with a compound
62% identity, 94% coverage: 4:217/228 of query aligns to 5:223/242 of 8itdC

query
sites
8itdC

L

L

V

V

L

L

I

I

R

R

H

H

G

G

Q

E

S

S

A

A

W

W

N

N

L

L

E

E

N

N

R

R

F
|
F

T

S

G

G

W

W

W

Y

D

D

V

A

N

D

L

L

T

S

P

P

Q

A

G

G

E

H

A

E

E

E

A

A

K

K

A

R

A

G

G

G

E

Q

L

A

L

L

A

R

E

D

R

A

G

G

L

Y

D

E

F

F

D

D

I

I

C

C

F

F

T

T

S

S

L

V

Q

Q

T

K

R

R

A

A

I

I

K

R

T

T

L

L

N

W

I

T

A

V

L

L

E

D

A

A

M

I

G

D

R

Q

L

M

W

W

L

L

P

P

V

V

E

V

K

R

H

T

W

W

R

R

L

L

N

N

E

E

R
|
R

H

H

Y

Y

G

G

L

L

T

T

G

G

L

L

D

N

K
|
K

A

A

E

E

T

T

A
|
A

A

A

K

K

H

H

S

G

A
x
E

E

A

Q

Q

V
|
V

H

K

I

I

W
|
W

R
|
R

R

R

S

S

F

Y

D

D

I

V

P

P

A

P

M

M

E

E

P

P

G

D

G

H

D

P

F

F

-

Y

-

S

D

N

L

I

T

S

N

K

D

D

R

R

Y

Y

A

A

G

D

I

L

-

T

-

E

-

D

E

Q

I

L

P

P

S

S

H

C

E

E

S

S

L

L

K

K

D

D

T

T

I

I

A

A

R

R

V

A

L

L

P

P

Y

F

W

W

E

N

E

E

R

E

I

I

I

V

P

P

E

Q

L

I

K

K

E

E

G

G

R

K

R

R

V

V

L

L

V

I

S

A

A

A

H

H

G

G

N

N

S

S

L

L

R

R

A

G

L

I

V

V

K

K

H

H

L

L

N

E

R

G

I

L

T

S

D

E

A

E

D

A

I

I

S

M

G

E

L

L

E

N

I

L

P

P

T

T

G

G

Q

I

P

P

I

I

V

V

F

Y

E

E

L

L

D

D

D

K

S

N

L

L

binding 4-(4-chloranyl-2-oxidanyl-phenyl)-2-[(4-phenylphenyl)sulfonylamino]benzoic acid: F21 (= F20), R89 (= R88), K99 (= K98), A103 (= A102), E108 (≠ A107), V111 (= V110), W114 (= W113), R115 (= R114)

8itcC Phosphoglycerate mutase 1 complexed with a compound
62% identity, 94% coverage: 4:217/228 of query aligns to 5:223/237 of 8itcC

query
sites
8itcC

L

L

V

V

L

L

I

I

R

R

H

H

G

G

Q

E

S

S

A

A

W

W

N

N

L

L

E

E

N

N

R

R

F

F

T

S

G

G

W

W

W

Y

D

D

V

A

N

D

L

L

T

S

P

P

Q

A

G

G

E

H

A

E

E

E

A

A

K

K

A

R

A

G

G

G

E

Q

L

A

L

L

A

R

E

D

R

A

G

G

L

Y

D

E

F

F

D

D

I

I

C

C

F

F

T

T

S

S

L

V

Q

Q

T

K

R

R

A

A

I

I

K

R

T

T

L

L

N

W

I

T

A

V

L

L

E

D

A

A

M

I

G

D

R

Q

L

M

W

W

L

L

P

P

V

V

E

V

K

R

H

T

W

W

R

R

L

L

N

N

E

E

R

R

H

H

Y

Y

G

G

L

L

T

T

G

G

L

L

D

N

K
|
K

A

A

E

E

T

T

A

A

K

K

H

H

S

G

A
x
E

E

A

Q

Q

V
|
V

H
x
K

I

I

W
|
W

R
|
R

S

S

F

Y

D

D

I

V

P

P

A

P

M

M

E

E

P

P

G

D

G

H

D

P

F

F

-

Y

-

S

D

N

L

I

T

S

N

K

D

D

R

R

Y

Y

A

A

G

D

I

L

-

T

-

E

-

D

E

Q

I

L

P

P

S

S

H

C

E

E

S

S

L

L

K

K

D

D

T

T

I

I

A

A

R

R

V

A

L

L

P

P

Y

F

W

W

E

N

E

E

R

E

I

I

I

V

P

P

E

Q

L

I

K

K

E

E

G

G

R

K

R

R

V

V

L

L

V

I

S

A

A

A

H

H

G

G

N

N

S

S

L

L

R

R

A

G

L

I

V

V

K

K

H

H

L

L

N

E

R

G

I

L

T

S

D

E

A

E

D

A

I

I

S

M

G

E

L

L

E

N

I

L

P

P

T

T

G

G

Q

I

P

P

I

I

V

V

F

Y

E

E

L

L

D

D

D

K

S

N

L

L

binding 5-(4-chlorophenyl)-3-[(4-phenylphenyl)sulfonylamino]phthalic acid: K99 (= K98), E108 (≠ A107), V111 (= V110), V111 (= V110), K112 (≠ H111), W114 (= W113), R115 (= R114), R116 (= R115)

8itbC Phosphoglycerate mutase 1 complexed with a compound
62% identity, 94% coverage: 4:217/228 of query aligns to 5:223/237 of 8itbC

query
sites
8itbC

L

L

V

V

L

L

I

I

R

R

H

H

G

G

Q

E

S

S

A

A

W

W

N

N

L

L

E

E

N
|
N

R
|
R

F
|
F

T

S

G

G

W

W

W

Y

D

D

V

A

N

D

L

L

T

S

P

P

Q

A

G

G

E

H

A

E

E

E

A

A

K

K

A

R

A

G

G

G

E

Q

L

A

L

L

A

R

E

D

R

A

G

G

L

Y

D

E

F

F

D

D

I

I

C

C

F

F

T

T

S

S

L

V

Q

Q

T

K

R

R

A

A

I

I

K

R

T

T

L

L

N

W

I

T

A

V

L

L

E

D

A

A

M

I

G

D

R

Q

L

M

W

W

L

L

P

P

V

V

E

V

K

R

H

T

W

W

R

R

L

L

N

N

E

E

R
|
R

H

H

Y

Y

G

G

L
|
L

T

T

G

G

L

L

D

N

K
|
K

A

A

E

E

T

T

A
|
A

A

A

K

K

H

H

S

G

A
x
E

E

A

Q

Q

V

V

H

K

I

I

W
|
W

R
|
R

R

R

S

S

F

Y

D

D

I

V

P

P

A

P

M

M

E

E

P

P

G

D

G

H

D

P

F

F

-

Y

-

S

D

N

L

I

T

S

N

K

D

D

R

R

Y

Y

A

A

G

D

I

L

-

T

-

E

-

D

E

Q

I

L

P

P

S

S

H

C

E

E

S

S

L

L

K

K

D

D

T

T

I

I

A

A

R

R

V

A

L

L

P

P

Y

F

W

W

E

N

E

E

R

E

I

I

I

V

P

P

E

Q

L

I

K

K

E

E

G

G

R

K

R

R

V

V

L

L

V

I

S

A

A

A

H

H

G

G

N

N

S

S

L

L

R
|
R

A

G

L

I

V

V

K

K

H

H

L

L

N

E

R

G

I

L

T

S

D

E

A

E

D

A

I

I

S

M

G

E

L

L

E

N

I

L

P

P

T

T

G

G

Q

I

P

P

I

I

V

V

F

Y

E

E

L

L

D

D

D

K

S

N

L

L

binding 5-(3-chloranyl-4-fluoranyl-phenyl)-2-oxidanyl-3-[(4-phenylphenyl)sulfonylamino]benzoic acid: N19 (= N18), R20 (= R19), F21 (= F20), R89 (= R88), L94 (= L93), K99 (= K98), K99 (= K98), A103 (= A102), E108 (≠ A107), W114 (= W113), R115 (= R114), R190 (= R184)

8it7C Phosphoglycerate mutase 1 complexed with a compound
62% identity, 94% coverage: 4:217/228 of query aligns to 5:223/237 of 8it7C

query
sites
8it7C

L

L

V

V

L

L

I

I

R

R

H

H

G

G

Q

E

S

S

A

A

W

W

N

N

L

L

E

E

N

N

R

R

F
|
F

T

S

G

G

W

W

W

Y

D

D

V

A

N

D

L

L

T

S

P

P

Q

A

G

G

E

H

A

E

E

E

A

A

K

K

A

R

A

G

G

G

E

Q

L

A

L

L

A

R

E

D

R

A

G

G

L

Y

D

E

F

F

D

D

I

I

C

C

F

F

T

T

S

S

L

V

Q

Q

T

K

R

R

A

A

I

I

K

R

T

T

L

L

N

W

I

T

A

V

L

L

E

D

A

A

M

I

G

D

R

Q

L

M

W

W

L

L

P

P

V

V

E

V

K

R

H

T

W

W

R

R

L

L

N

N

E

E

R
|
R

H

H

Y

Y

G

G

L
|
L

T

T

G

G

L

L

D

N

K
|
K

A

A

E

E

T

T

A
|
A

A

A

K

K

H

H

S

G

A
x
E

E

A

Q

Q

V
|
V

H
x
K

I

I

W
|
W

R
|
R

R

R

S

S

F

Y

D

D

I

V

P

P

A

P

M

M

E

E

P

P

G

D

G

H

D

P

F

F

-

Y

-

S

D

N

L

I

T

S

N

K

D

D

R

R

Y

Y

A

A

G

D

I

L

-

T

-

E

-

D

E

Q

I

L

P

P

S

S

H

C

E

E

S

S

L

L

K

K

D

D

T

T

I

I

A

A

R

R

V

A

L

L

P

P

Y

F

W

W

E

N

E

E

R

E

I

I

I

V

P

P

E

Q

L

I

K

K

E

E

G

G

R

K

R

R

V

V

L

L

V

I

S

A

A

A

H

H

G

G

N

N

S

S

L

L

R

R

A

G

L

I

V

V

K

K

H

H

L

L

N

E

R

G

I

L

T

S

D

E

A

E

D

A

I

I

S

M

G

E

L

L

E

N

I

L

P

P

T

T

G

G

Q

I

P

P

I

I

V

V

F

Y

E

E

L

L

D

D

D

K

S

N

L

L

binding 4-(4-chlorophenyl)-2-oxidanyl-6-[(4-phenylphenyl)sulfonylamino]benzoic acid: F21 (= F20), R89 (= R88), L94 (= L93), K99 (= K98), A103 (= A102), E108 (≠ A107), V111 (= V110), K112 (≠ H111), W114 (= W113), R115 (= R114)

8it6C Phosphoglycerate mutase 1 complexed with a compound
62% identity, 94% coverage: 4:217/228 of query aligns to 5:223/237 of 8it6C

query
sites
8it6C

L

L

V

V

L

L

I

I

R

R

H

H

G

G

Q

E

S

S

A

A

W

W

N

N

L

L

E

E

N

N

R

R

F
|
F

T

S

G

G

W

W

W

Y

D

D

V

A

N

D

L

L

T

S

P

P

Q

A

G

G

E

H

A

E

E

E

A

A

K

K

A

R

A

G

G

G

E

Q

L

A

L

L

A

R

E

D

R

A

G

G

L

Y

D

E

F

F

D

D

I

I

C

C

F

F

T

T

S

S

L

V

Q

Q

T

K

R

R

A

A

I

I

K

R

T

T

L

L

N

W

I

T

A

V

L

L

E

D

A

A

M

I

G

D

R

Q

L

M

W

W

L

L

P

P

V

V

E

V

K

R

H

T

W

W

R

R

L

L

N

N

E

E

R
|
R

H

H

Y
|
Y

G

G

L

L

T

T

G

G

L

L

D

N

K
|
K

A

A

E

E

T

T

A
|
A

A

A

K

K

H

H

S

G

A
x
E

E

A

Q

Q

V
|
V

H
x
K

I

I

W
|
W

R
|
R

R

R

S

S

F

Y

D

D

I

V

P

P

P
|
P

P

P

A

P

M

M

E

E

P

P

G

D

G

H

D

P

F

F

-

Y

-

S

D

N

L

I

T

S

N

K

D

D

R

R

Y

Y

A

A

G

D

I

L

-

T

-

E

-

D

E

Q

I

L

P

P

S

S

H

C

E

E

S

S

L

L

K

K

D

D

T

T

I

I

A

A

R

R

V

A

L

L

P

P

Y

F

W

W

E

N

E

E

R

E

I

I

I

V

P

P

E

Q

L

I

K

K

E

E

G

G

R

K

R

R

V

V

L

L

V

I

S

A

A

A

H

H

G

G

N

N

S

S

L

L

R

R

A

G

L

I

V

V

K

K

H

H

L

L

N

E

R

G

I

L

T

S

D

E

A

E

D

A

I

I

S

M

G

E

L

L

E

N

I

L

P

P

T

T

G

G

Q

I

P

P

I

I

V

V

F

Y

E

E

L

L

D

D

D

K

S

N

L

L

binding 4-[4-chloranyl-2-(2-hydroxy-2-oxoethyloxy)phenyl]-2-[(4-phenylphenyl)sulfonylamino]benzoic acid: F21 (= F20), R89 (= R88), Y91 (= Y90), K99 (= K98), A103 (= A102), E108 (≠ A107), V111 (= V110), K112 (≠ H111), W114 (= W113), R115 (= R114), P122 (= P121)

8it5C Phosphoglycerate mutase 1 complexed with a compound
62% identity, 94% coverage: 4:217/228 of query aligns to 5:223/237 of 8it5C

query
sites
8it5C

L

L

V

V

L

L

I

I

R

R

H

H

G

G

Q

E

S

S

A

A

W

W

N

N

L

L

E

E

N
|
N

R
|
R

F
|
F

T

S

G

G

W

W

W

Y

D

D

V

A

N

D

L

L

T

S

P

P

Q

A

G

G

E

H

A

E

E

E

A

A

K

K

A

R

A

G

G

G

E

Q

L

A

L

L

A

R

E

D

R

A

G

G

L

Y

D

E

F

F

D

D

I

I

C

C

F

F

T

T

S

S

L

V

Q

Q

T

K

R

R

A

A

I

I

K

R

T

T

L

L

N

W

I

T

A

V

L

L

E

D

A

A

M

I

G

D

R

Q

L

M

W

W

L

L

P

P

V

V

E

V

K

R

H

T

W

W

R

R

L

L

N

N

E

E

R
|
R

H

H

Y
|
Y

G

G

L

L

T

T

G

G

L

L

D

N

K
|
K

A

A

E

E

T

T

A

A

K

K

H

H

S

G

A

E

E

A

Q

Q

V
|
V

H

K

I

I

W

W

R
|
R

R

R

S

S

F

Y

D

D

I

V

P

P

P
|
P

P

P

A

P

M

M

E

E

P

P

G

D

G

H

D

P

F

F

-

Y

-

S

D

N

L

I

T

S

N

K

D

D

R

R

Y

Y

A

A

G

D

I

L

-

T

-

E

-

D

E

Q

I

L

P

P

S

S

H

C

E

E

S

S

L

L

K

K

D

D

T

T

I

I

A

A

R

R

V

A

L

L

P

P

Y

F

W

W

E

N

E

E

R

E

I

I

I

V

P

P

E

Q

L

I

K

K

E

E

G

G

R

K

R

R

V

V

L

L

V

I

S

A

A

A

H

H

G

G

N

N

S

S

L

L

R

R

A

G

L

I

V

V

K

K

H

H

L

L

N

E

R

G

I

L

T

S

D

E

A

E

D

A

I

I

S

M

G

E

L

L

E

N

I

L

P

P

T

T

G

G

Q

I

P

P

I

I

V

V

F

Y

E

E

L

L

D

D

D

K

S

N

L

L

binding 5-(4-chlorophenyl)-3-[(4-phenylphenyl)sulfonylamino]thiophene-2-carboxylic acid: N19 (= N18), R20 (= R19), F21 (= F20), R89 (= R88), Y91 (= Y90), K99 (= K98), V111 (= V110), R115 (= R114), P122 (= P121)

5y35C Phosphoglycerate mutase 1 complexed with a small molecule inhibitor kh1
62% identity, 94% coverage: 4:217/228 of query aligns to 5:223/237 of 5y35C

query
sites
5y35C

L

L

V

V

L

L

I

I

R

R

H
|
H

G

G

Q

E

S

S

A

A

W

W

N

N

L

L

E

E

N
|
N

R

R

F
|
F

T

S

G

G

W

W

W

Y

D

D

V

A

N

D

L

L

T

S

P

P

Q

A

G

G

E

H

A

E

E

E

A

A

K

K

A

R

A

G

G

G

E

Q

L

A

L

L

A

R

E

D

R

A

G

G

L

Y

D

E

F

F

D

D

I

I

C

C

F

F

T

T

S

S

L

V

Q

Q

T

K

R
|
R

A

A

I

I

K

R

T

T

L

L

N

W

I

T

A

V

L

L

E

D

A

A

M

I

G

D

R

Q

L

M

W

W

L

L

P

P

V

V

E

V

K

R

H

T

W

W

R

R

L

L

N

N

E
|
E

R
|
R

H

H

Y

Y

G

G

L

L

T

T

G

G

L

L

D

N

K
|
K

A

A

E

E

T

T

A

A

K

K

H

H

S

G

A

E

E

A

Q

Q

V

V

H

K

I

I

W
|
W

R
|
R

R

R

S

S

F

Y

D

D

I

V

P

P

A

P

M

M

E

E

P

P

G

D

G

H

D

P

F

F

-

Y

-

S

D

N

L

I

T

S

N

K

D

D

R

R

Y

Y

A

A

G

D

I

L

-

T

-

E

-

D

E

Q

I

L

P

P

S

S

H

C

E

E

S

S

L

L

K

K

D

D

T

T

I

I

A

A

R

R

V

A

L

L

P

P

Y

F

W

W

E

N

E

E

R

E

I

I

I

V

P

P

E

Q

L

I

K

K

E

E

G

G

R

K

R

R

V

V

L

L

V

I

S

A

A

A

H
|
H

G

G

N

N

S

S

L

L

R

R

A

G

L

I

V

V

K

K

H

H

L

L

N

E

R

G

I

L

T

S

D

E

A

E

D

A

I

I

S

M

G

E

L

L

E

N

I

L

P

P

T

T

G

G

Q

I

P

P

I

I

V

V

F

Y

E

E

L

L

D

D

D

K

S

N

L

L

active site: H10 (= H9), R61 (= R60), E88 (= E87), H185 (= H179)
binding ~{N}-[3,4-bis(oxidanyl)-9,10-bis(oxidanylidene)anthracen-2-yl]-4-methyl-benzenesulfonamide: N19 (= N18), F21 (= F20), R89 (= R88), K99 (= K98), W114 (= W113), R115 (= R114)

7xb9B Phosphoglycerate mutase 1 complexed with a covalent inhibitor
62% identity, 94% coverage: 4:217/228 of query aligns to 4:222/231 of 7xb9B

query
sites
7xb9B

L

L

V

V

L

L

I

I

R

R

H

H

G

G

Q

E

S

S

A

A

W

W

N

N

L

L

E

E

N

N

R

R

F
|
F

T

S

G

G

W

W

W

Y

D

D

V

A

N

D

L

L

T

S

P

P

Q

A

G

G

E

H

A

E

E

E

A

A

K

K

A

R

A

G

G

G

E

Q

L

A

L

L

A

R

E

D

R

A

G

G

L

Y

D

E

F

F

D

D

I

I

C

C

F

F

T

T

S

S

L

V

Q

Q

T

K

R

R

A

A

I

I

K

R

T

T

L

L

N

W

I

T

A

V

L

L

E

D

A

A

M

I

G

D

R

Q

L

M

W

W

L

L

P

P

V

V

E

V

K

R

H

T

W

W

R

R

L

L

N

N

E

E

R
|
R

H

H

Y
|
Y

G

G

L

L

T

T

G

G

L

L

D

N

K
|
K

A

A

E

E

T

T

A

A

K

K

H

H

S

G

A

E

E

A

Q

Q

V

V

H

K

I

I

W
|
W

R
|
R

R

R

S

S

F

Y

D

D

I

V

P

P

A

P

M

M

E

E

P

P

G

D

G

H

D

P

F

F

-

Y

-

S

D

N

L

I

T

S

N

K

D

D

R

R

Y

Y

A

A

G

D

I

L

-

T

-

E

-

D

E

Q

I

L

P

P

S

S

H

C

E

E

S

S

L

L

K

K

D

D

T

T

I

I

A

A

R

R

V

A

L

L

P

P

Y

F

W

W

E

N

E

E

R

E

I

I

I

V

P

P

E

Q

L

I

K

K

E

E

G

G

R

K

R

R

V

V

L

L

V

I

S

A

A

A

H

H

G

G

N
|
N

S

S

L

L

R

R

A

G

L

I

V

V

K

K

H

H

L

L

N

E

R

G

I

L

T

S

D

E

A

E

D

A

I

I

S

M

G

E

L

L

E

N

I

L

P

P

T

T

G

G

Q

I

P

P

I

I

V

V

F

Y

E

E

L

L

D

D

D

K

S

N

L

L

binding 3-[[5-(cyclopenten-1-yl)-2-methoxycarbonyl-phenyl]sulfamoyl]benzenesulfonic acid: F20 (= F20), R88 (= R88), Y90 (= Y90), K98 (= K98), W113 (= W113), R114 (= R114), N186 (= N181)

6isnC Phosphoglycerate mutase 1 complexed with a small molecule inhibitor
62% identity, 94% coverage: 4:217/228 of query aligns to 5:223/234 of 6isnC

query
sites
6isnC

L

L

V

V

L

L

I

I

R

R

H

H

G

G

Q

E

S

S

A

A

W

W

N

N

L

L

E

E

N
|
N

R
|
R

F
|
F

T

S

G

G

W

W

W

Y

D

D

V

A

N

D

L

L

T

S

P

P

Q

A

G

G

E

H

A

E

E

E

A

A

K

K

A

R

A

G

G

G

E

Q

L

A

L

L

A

R

E

D

R

A

G

G

L

Y

D

E

F

F

D

D

I

I

C

C

F

F

T

T

S

S

L

V

Q

Q

T

K

R

R

A

A

I

I

K

R

T

T

L

L

N

W

I

T

A

V

L

L

E

D

A

A

M

I

G

D

R

Q

L

M

W

W

L

L

P

P

V

V

E

V

K

R

H

T

W

W

R

R

L

L

N

N

E

E

R
|
R

H

H

Y

Y

G

G

L

L

T

T

G

G

L

L

D

N

K
|
K

A

A

E

E

T

T

A

A

K

K

H

H

S

G

A

E

E

A

Q

Q

V

V

H

K

I

I

W

W

R
|
R

R

R

S

S

F

Y

D

D

I

V

P

P

A

P

M

M

E

E

P

P

G

D

G

H

D

P

F

F

-

Y

-

S

D

N

L

I

T

S

N

K

D

D

R

R

Y

Y

A

A

G

D

I

L

-

T

-

E

-

D

E

Q

I

L

P

P

S

S

H

C

E

E

S

S

L

L

K

K

D

D

T

T

I

I

A

A

R

R

V

A

L

L

P

P

Y

F

W

W

E

N

E

E

R

E

I

I

I

V

P

P

E

Q

L

I

K

K

E

E

G

G

R

K

R

R

V

V

L

L

V

I

S

A

A

A

H

H

G

G

N

N

S

S

L

L

R

R

A

G

L

I

V

V

K

K

H

H

L

L

N

E

R

G

I

L

T

S

D

E

A

E

D

A

I

I

S

M

G

E

L

L

E

N

I

L

P

P

T

T

G

G

Q

I

P

P

I

I

V

V

F

Y

E

E

L

L

D

D

D

K

S

N

L

L

binding 4-chloro-N-(3,4-dihydroxy-9,10-dioxo-9,10-dihydroanthracen-2-yl)benzene-1-sulfonamide: N19 (= N18), R20 (= R19), F21 (= F20), R89 (= R88), K99 (= K98), R115 (= R114)

Query Sequence

>WP_085218187.1 NCBI__GCF_900177405.1:WP_085218187.1
MPTLVLIRHGQSAWNLENRFTGWWDVNLTPQGEAEAKAAGELLAERGLDFDICFTSLQTR
AIKTLNIALEAMGRLWLPVEKHWRLNERHYGGLTGLDKAETAAKHSAEQVHIWRRSFDIP
PPAMEPGGDFDLTNDRRYAGIEIPSHESLKDTIARVLPYWEERIIPELKEGRRVLVSAHG
NSLRALVKHLNRITDADISGLEIPTGQPIVFELDDSLDAKDFYYLKDR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory