SitesBLAST
Comparing WP_085218423.1 NCBI__GCF_900177405.1:WP_085218423.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5itvA Crystal structure of bacillus subtilis bacc dihydroanticapsin 7- dehydrogenase in complex with nadh (see paper)
36% identity, 96% coverage: 9:250/251 of query aligns to 10:253/255 of 5itvA
- active site: G18 (= G17), S141 (= S133), Y154 (= Y146), K158 (= K150)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G13), S17 (= S16), G18 (= G17), I19 (= I18), D38 (= D38), I39 (≠ R39), T61 (vs. gap), I63 (≠ V62), N89 (= N82), G91 (= G84), T139 (= T131), S141 (= S133), Y154 (= Y146), K158 (= K150), P184 (= P176), G185 (= G177), I186 (≠ G178), I187 (≠ V179)
6y0sAAA R-specific alcohol dehydrogenase (see paper)
33% identity, 98% coverage: 4:250/251 of query aligns to 4:249/251 of 6y0sAAA
1zk1A Structure of r-specific alcohol dehydrogenase (mutant g37d) from lactobacillus brevis in complex with phenylethanol and NAD (see paper)
33% identity, 98% coverage: 4:250/251 of query aligns to 4:249/251 of 1zk1A
- active site: G17 (= G17), S142 (= S133), Y155 (= Y146), K159 (= K150)
- binding 1-phenylethanone: A93 (= A86), N95 (≠ G88), Y155 (= Y146), Y189 (≠ G178)
- binding nicotinamide-adenine-dinucleotide: G13 (= G13), L16 (≠ S16), I18 (= I18), D37 (= D38), H61 (≠ G60), D62 (= D61), S63 (≠ V62), N89 (= N82), A90 (= A83), I92 (= I85), M140 (≠ T131), Y155 (= Y146), G188 (= G177), I190 (≠ V179), L194 (≠ I183)
1zjzA Structure of r-specific alcohol dehydrogenase (mutant g37d) from lactobacillus brevis in complex with phenylethanol and NAD (see paper)
33% identity, 98% coverage: 4:250/251 of query aligns to 4:249/251 of 1zjzA
- active site: G17 (= G17), S142 (= S133), Y155 (= Y146), K159 (= K150)
- binding nicotinamide-adenine-dinucleotide: G13 (= G13), L16 (≠ S16), I18 (= I18), D37 (= D38), D62 (= D61), N89 (= N82), A90 (= A83), G91 (= G84), I92 (= I85), Y155 (= Y146), G188 (= G177), I190 (≠ V179), L194 (≠ I183)
- binding (1r)-1-phenylethanol: A93 (= A86), N95 (≠ G88), L152 (≠ T143), Y155 (= Y146)
1zjyA Structure of r-specific alcohol dehydrogenase (mutant g37d) from lactobacillus brevis in complex with phenylethanol and nadh (see paper)
33% identity, 98% coverage: 4:250/251 of query aligns to 4:249/251 of 1zjyA
- active site: G17 (= G17), S142 (= S133), Y155 (= Y146), K159 (= K150)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G13 (= G13), L16 (≠ S16), G17 (= G17), I18 (= I18), D37 (= D38), D62 (= D61), N89 (= N82), A90 (= A83), G91 (= G84), I92 (= I85), Y155 (= Y146), G188 (= G177), I190 (≠ V179), L194 (≠ I183)
- binding (1r)-1-phenylethanol: A93 (= A86), N95 (≠ G88), L152 (≠ T143), Y155 (= Y146), Y189 (≠ G178)
6ixmC Crystal structure of the ketone reductase chkred20 from the genome of chryseobacterium sp. Ca49 complexed with NAD (see paper)
33% identity, 98% coverage: 4:250/251 of query aligns to 3:246/248 of 6ixmC
- active site: G16 (= G17), S142 (= S133), Y155 (= Y146), K159 (= K150)
- binding nicotinamide-adenine-dinucleotide: G12 (= G13), S15 (= S16), G16 (= G17), I17 (= I18), D36 (= D38), I37 (≠ R39), A61 (≠ G60), D62 (= D61), T63 (≠ V62), N89 (= N82), A90 (= A83), M140 (vs. gap), S142 (= S133), Y155 (= Y146), K159 (= K150), P185 (= P176), A186 (≠ G177), Y187 (≠ G178), I188 (≠ V179), L192 (≠ I183)
1zk4A Structure of r-specific alcohol dehydrogenase (wildtype) from lactobacillus brevis in complex with acetophenone and NADP (see paper)
33% identity, 98% coverage: 4:250/251 of query aligns to 4:249/251 of 1zk4A
- active site: G17 (= G17), S142 (= S133), Y155 (= Y146), K159 (= K150)
- binding 1-phenylethanone: A93 (= A86), Y155 (= Y146), Y189 (≠ G178)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G13), T15 (≠ A15), L16 (≠ S16), I18 (= I18), T36 (≠ L36), G37 (≠ A37), R38 (vs. gap), H61 (≠ G60), D62 (= D61), N89 (= N82), A90 (= A83), G91 (= G84), I92 (= I85), Y155 (= Y146), G188 (= G177), I190 (≠ V179), T192 (= T181), L194 (≠ I183)
5t5qC Crystal structure of short-chain dehydrogenase/reductase sdr:glucose/ribitol dehydrogenase from brucella melitensis
36% identity, 98% coverage: 3:248/251 of query aligns to 4:241/245 of 5t5qC
- active site: G18 (= G17), S140 (= S133), N150 (≠ T143), Y153 (= Y146), K157 (= K150)
- binding nicotinamide-adenine-dinucleotide: N16 (≠ A15), G17 (≠ S16), G18 (= G17), I19 (= I18), D38 (= D38), L39 (≠ R39), D63 (= D64), A64 (≠ E65), S90 (≠ N82), I113 (≠ V105), Y153 (= Y146), K157 (= K150), P182 (= P176), I185 (≠ V179), T187 (= T181), M189 (≠ I183)
H9XP47 Meso-2,3-butanediol dehydrogenase; BDH; meso-2,3-BDH; (R,S)-butane-2,3-diol dehydrogenase; NAD(H)-dependent meso-2,3-BDH; SmBdh; EC 1.1.1.- from Serratia marcescens (see paper)
33% identity, 98% coverage: 4:250/251 of query aligns to 3:242/251 of H9XP47
- N15 (≠ S16) binding NAD(+)
- M17 (≠ I18) binding NAD(+)
- D36 (= D38) binding NAD(+)
- D60 (= D61) binding NAD(+)
- V61 (= V62) binding NAD(+)
- N87 (vs. gap) binding NAD(+)
- S138 (= S133) binding (R)-acetoin; binding (S)-acetoin
- V139 (≠ A134) mutation to Q: Retains 50% of activity with acetoin as substrate; when associated with A-247.
- S140 (= S135) binding (R)-acetoin
- Y151 (= Y146) binding (R)-acetoin; binding (S)-acetoin; binding NAD(+)
- K155 (= K150) binding NAD(+)
- V184 (= V179) binding NAD(+)
- T186 (= T181) binding NAD(+)
- RDK 197:199 (≠ KAS 194:196) mutation to SEAAGKPLGYGTET: Mimics longer alpha6 helix. Retains 3% of activity with acetoin as substrate.
Sites not aligning to the query:
- 247 Q→A: Retains 10% of activity with acetoin as substrate. Retains 50% of activity with acetoin as substrate; when associated with Q-139.
6vspB Structure of serratia marcescens 2,3-butanediol dehydrogenase mutant q247a (see paper)
33% identity, 98% coverage: 4:250/251 of query aligns to 5:244/252 of 6vspB
6xewA Structure of serratia marcescens 2,3-butanediol dehydrogenase (see paper)
33% identity, 98% coverage: 4:250/251 of query aligns to 3:242/251 of 6xewA
- active site: G16 (= G17), S138 (= S133), Y151 (= Y146)
- binding r,3-hydroxybutan-2-one: S138 (= S133), S140 (= S135), Y151 (= Y146)
- binding s,3-hydroxybutan-2-one: S138 (= S133), Y151 (= Y146), S182 (≠ G177)
- binding nicotinamide-adenine-dinucleotide: G12 (= G13), N15 (≠ S16), G16 (= G17), M17 (≠ I18), D36 (= D38), W37 (≠ R39), W37 (≠ R39), A38 (≠ D40), I59 (vs. gap), D60 (= D61), V61 (= V62), N87 (vs. gap), A88 (= A83), G89 (= G84), V110 (= V105), T136 (= T131), S138 (= S133), Y151 (= Y146), K155 (= K150), S182 (≠ G177), L183 (≠ G178), V184 (= V179), T186 (= T181), N187 (≠ P182), M188 (≠ I183), T189 (≠ W184)
6vspA Structure of serratia marcescens 2,3-butanediol dehydrogenase mutant q247a (see paper)
33% identity, 98% coverage: 4:250/251 of query aligns to 3:242/251 of 6vspA
- active site: G16 (= G17), S138 (= S133), Y151 (= Y146)
- binding nicotinamide-adenine-dinucleotide: G12 (= G13), N15 (≠ S16), G16 (= G17), M17 (≠ I18), D36 (= D38), W37 (≠ R39), W37 (≠ R39), A38 (≠ D40), I59 (vs. gap), D60 (= D61), V61 (= V62), N87 (vs. gap), A88 (= A83), G89 (= G84), V90 (≠ I85), V110 (= V105), T136 (= T131), S138 (= S133), Y151 (= Y146), K155 (= K150), P181 (= P176), S182 (≠ G177), L183 (≠ G178), V184 (= V179), T186 (= T181), N187 (≠ P182), M188 (≠ I183), T189 (≠ W184)
6f9qC Binary complex of a 7s-cis-cis-nepetalactol cyclase from nepeta mussinii with NAD+ (see paper)
35% identity, 98% coverage: 3:248/251 of query aligns to 5:253/260 of 6f9qC
- active site: G19 (= G17), Y159 (= Y146)
- binding nicotinamide-adenine-dinucleotide: G15 (= G13), S18 (= S16), G19 (= G17), I20 (= I18), D40 (= D38), I41 (≠ R39), Q42 (≠ D40), C63 (≠ G60), D64 (= D61), V65 (= V62), N91 (= N82), A92 (= A83), G93 (= G84), N144 (≠ T131), Y159 (= Y146), K163 (= K150), P189 (= P176), M190 (≠ G177), V192 (= V179), T194 (= T181), P195 (= P182), L196 (≠ I183), T197 (≠ W184)
A0A3Q8GLE8 (+)-cis,cis-nepetalactol synthase NEPS3; Nepetalactol-related short-chain reductase 3; NmNEPS3; EC 5.5.1.35 from Nepeta racemosa (Catmint) (Raceme catnip) (see paper)
35% identity, 98% coverage: 3:248/251 of query aligns to 11:259/270 of A0A3Q8GLE8
- 21:27 (vs. 13:19, 86% identical) binding NAD(+)
- DIQ 46:48 (≠ DRD 38:40) binding NAD(+)
- DV 70:71 (= DV 61:62) binding NAD(+)
- N97 (= N82) binding NAD(+)
- N150 (≠ T131) mutation to T: No effect on catalytic activity.
- S154 (= S135) mutation to L: Abolishes catalytic activity.
- YVMSK 165:169 (≠ YGASK 146:150) binding NAD(+)
- K169 (= K150) mutation to M: Abolishes catalytic activity.
- M196 (≠ G177) mutation to S: Abolishes catalytic activity.
- VATPL 198:202 (≠ VETPI 179:183) binding NAD(+)
7ejiB Crystal structure of kred f147l/l153q/y190p/l199a/m205f/m206f variant and methyl methacrylate complex
34% identity, 97% coverage: 8:250/251 of query aligns to 8:249/251 of 7ejiB
- binding methyl 2-methylprop-2-enoate: S142 (= S133), I143 (≠ A134), Y155 (= Y146), F205 (= F204)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G13), T15 (≠ A15), L16 (≠ S16), G17 (= G17), I18 (= I18), R38 (= R39), H39 (≠ D40), D62 (= D61), A63 (≠ V62), N89 (= N82), A90 (= A83), V112 (= V105), M140 (≠ T131), S142 (= S133), Y155 (= Y146), K159 (= K150), P187 (= P176), P189 (≠ G178), I190 (≠ V179), T192 (= T181), P193 (= P182), L194 (≠ I183)
7ejhA Crystal structure of kred mutant-f147l/l153q/y190p/l199a/m205f/m206f and 2-hydroxyisoindoline-1,3-dione complex
34% identity, 97% coverage: 8:250/251 of query aligns to 10:251/253 of 7ejhA