SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_085219145.1 NCBI__GCF_900177405.1:WP_085219145.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4yerA Crystal structure of an abc transporter atp-binding protein (tm_1403) from thermotoga maritima msb8 at 2.35 a resolution
35% identity, 78% coverage: 1:242/311 of query aligns to 1:245/285 of 4yerA

query
sites
4yerA

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binding adenosine-5'-diphosphate: F14 (≠ Y14), F17 (≠ L18), N39 (= N40), G40 (= G41), G42 (= G43), K43 (= K44), T44 (= T45), T45 (= T46), T135 (≠ E134), F136 (≠ L135), S137 (= S136)

7roqA Alternative structure of human abca1
34% identity, 93% coverage: 17:306/311 of query aligns to 783:1082/1831 of 7roqA

query
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7roqA

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Sites not aligning to the query:

binding cholesterol: 688, 1106, 1426, 1526, 1527

5x40A Structure of a cbio dimer bound with amppcp (see paper)
36% identity, 76% coverage: 1:235/311 of query aligns to 1:248/280 of 5x40A

query
sites
5x40A

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binding phosphomethylphosphonic acid adenylate ester: F14 (≠ Y14), V18 (≠ L18), A20 (= A20), N40 (= N40), G41 (= G41), G43 (= G43), K44 (= K44), S45 (≠ T45), T46 (= T46), Q88 (= Q85), H139 (≠ Q133), M140 (≠ E134), L141 (= L135), S142 (= S136), G144 (= G138), Q145 (≠ M139), Q166 (≠ E160), H198 (= H192)
binding magnesium ion: S45 (≠ T45), Q88 (= Q85)

7tbwA The structure of atp-bound abca1 (see paper)
35% identity, 98% coverage: 5:309/311 of query aligns to 754:1053/1928 of 7tbwA

query
sites
7tbwA

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binding adenosine-5'-triphosphate: Y763 (= Y14), D765 (≠ S16), K768 (vs. gap), N790 (= N40), G791 (= G41), G793 (= G43), K794 (= K44), T795 (= T45), Q835 (= Q85), Q887 (≠ E134), S889 (= S136), H944 (= H192)
binding magnesium ion: Q835 (= Q85), D912 (= D159), Q913 (≠ E160)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 1630, 1633, 1657, 1658, 1660, 1661, 1662, 1663, 1702, 1753, 1755, 1756, 1757, 1779, 1783, 1811
binding cholesterol: 218, 219, 360, 367
binding magnesium ion: 1662, 1702

8ee6A Cryo-em structure of human abca7 in pe/ch nanodiscs (see paper)
34% identity, 98% coverage: 1:306/311 of query aligns to 620:934/1808 of 8ee6A

query
sites
8ee6A

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binding phosphothiophosphoric acid-adenylate ester: F633 (≠ Y14), A640 (= A20), N660 (= N40), G661 (= G41), G663 (= G43), K664 (= K44), T665 (= T45), T666 (= T46)

Sites not aligning to the query:

binding phosphothiophosphoric acid-adenylate ester: 607, 608, 1507, 1514, 1535, 1536, 1537, 1538, 1539

8f5bA Human abca4 structure in complex with amp-pnp
32% identity, 97% coverage: 3:303/311 of query aligns to 719:1028/1924 of 8f5bA

query
sites
8f5bA

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binding phosphoaminophosphonic acid-adenylate ester: N757 (= N40), G758 (= G41), G760 (= G43), K761 (= K44), T762 (= T45), T763 (= T46), Q802 (= Q85), D853 (≠ E134)
binding magnesium ion: T762 (= T45), Q802 (= Q85)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 1652, 1653, 1654, 1655, 1656, 1657, 1697, 1748, 1750
binding magnesium ion: 1657, 1697

O95477 Phospholipid-transporting ATPase ABCA1; ATP-binding cassette sub-family A member 1; ATP-binding cassette transporter 1; ABC-1; ATP-binding cassette 1; Cholesterol efflux regulatory protein; EC 7.6.2.1 from Homo sapiens (Human) (see 35 papers)
38% identity, 71% coverage: 5:226/311 of query aligns to 899:1123/2261 of O95477

query
sites
O95477

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D917 (≠ N22) to Y: in a colorectal cancer sample; somatic mutation
T929 (≠ F34) to I: in TGD; moderately decreased protein abundance; highly decreased ATPase activity; highly decreased phospholipid translocase activity; loss protein subcellular localization to the plasma membrane
N935 (= N40) to S: in TGD; moderately decreased protein abundance; highly decreased ATPase activity; highly decreased phospholipid translocase activity; dbSNP:rs28937313
K939 (= K44) mutation to M: Inhibits ATPase activity; when associated with M-1952. Decreases translocase activity; when associated with M-1952. Does not affect protein subcellular localization in plasma membrane and endosome; when associated with M-1952.
S1042 (≠ M144) modified: Phosphoserine; by PKA
P1065 (≠ V167) natural variant: P -> S
M1091 (≠ I194) to T: in FHA1; loss of localization to plasma membrane; decreased cholesterol efflux; decreased phospholipid efflux
C1110 (≠ I213) modified: S-palmitoyl cysteine; mutation to S: Decreased palmitoylation; when associated with S-3, S-23 and S-1111.
C1111 (≠ L214) modified: S-palmitoyl cysteine; mutation to S: Decreased palmitoylation; when associated with S-3, S-23 and S-1110.

Sites not aligning to the query:

3 modified: S-palmitoyl cysteine; C→S: Mild decrease of palmitoylation. Loss of localization to plasma membrane. Decreased cholesterol efflux. Decreased phospholipid efflux. Decreased palmitoylation; when associated with S-23, S-1110 and S-1111.
23 modified: S-palmitoyl cysteine; C→S: Mild decrease of palmitoylation. Loss of localization to plasma membrane. Decreased palmitoylation; when associated with S-3, S-1110 and S-1111.
74 I→C: 85-90% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane; when associated with C-371.; I→K: 85-90% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane; when associated with E-371.
75 modified: Disulfide link with 309
85 P → L: in FHA1; Alabama; dbSNP:rs145183203
98 modified: carbohydrate, N-linked (GlcNAc...) asparagine
100 S→C: Highly decreased protein abundance. Highly decreased ATPase activity. Highly decreased phospholipid translocase activity.
210 E → D: in a colorectal cancer sample; somatic mutation
219 R → K: in dbSNP:rs2230806
230 R → C: in dbSNP:rs9282541
244 modified: carbohydrate, N-linked (GlcNAc...) asparagine
248 P → A: in dbSNP:rs142625938
255 A → T: in TGD; deficient cellular cholesterol efflux; dbSNP:rs758100110
304 V→C: No effect on phospholipid and cholesterol efflux or localization to cell membrane; when associated with C-308.
308 V→C: No effect on phospholipid and cholesterol efflux or localization to cell membrane; when associated with C-304.
309 modified: Disulfide link with 75
364 S → C: in dbSNP:rs775035559
371 I→C: No effect on phospholipid and cholesterol efflux or localization to cell membrane. 85-90% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane; when associated with C-74 or C-375.; I→E: 85-90% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane; when associated with K-74.
375 L→C: 85-90% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane; when associated with C-371.
399 V → A: in dbSNP:rs9282543
401 K → Q: in dbSNP:rs138487227
496 R → W: in dbSNP:rs147675550
568 K→A: 60-65% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane.
573 Y→F: No effect on phospholipid and cholesterol efflux and on localization to cell membrane.
581 D→K: 80-85% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane; when associated with K-584 and K-585.
583 F→K: 90-95% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane; when associated with E-590.
584 E→K: 80-85% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane; when associated with K-581 and K-585.
585 D→K: 80-85% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane; when associated with K-581 and K-584.
590 W → S: in TGD; moderately decreased protein abundance; highly decreased ATPase activity; highly decreased phospholipid translocase activity; dbSNP:rs137854496; W→E: 90-95% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane; when associated with K-583.
593 F→L: Moderately decreased protein abundance. Highly decreased ATPase activity. Highly decreased phospholipid translocase activity.
638 R → Q: in dbSNP:rs374190304
771 V → M: in dbSNP:rs2066718
774 T → P: in dbSNP:rs35819696; natural variant: T -> S
776 K → N: in dbSNP:rs138880920
815 E → G: in dbSNP:rs145582736
825 V → I: in dbSNP:rs2066715
883 I → M: in dbSNP:rs2066714
1172 E → D: in dbSNP:rs33918808
1181 S → F: in dbSNP:rs76881554
1216 G → V: in dbSNP:rs562403512
1341 R → T: in dbSNP:rs147743782
1376 S → G: in dbSNP:rs145689805
1379 L → F: in TGD; the mutant protein is retained in the endoplasmic reticulum while the wild-type protein is located at the plasma membrane
1407 A → T: in a colorectal cancer sample; somatic mutation; dbSNP:rs189206655
1463 modified: Disulfide link with 1477
1477 modified: Disulfide link with 1463; C → R: in TGD; loss of interaction with APOE; unable to generate APOE-containing high density lipoproteins; moderately decreased protein abundance; moderately decreased ATPase activity; moderately decreased phospholipid translocase activity; dbSNP:rs137854494
1512 T→M: Moderately decreased protein abundance. Does not affect ATPase activity. Moderately decreased phospholipid translocase activity.
1555 I → T: in dbSNP:rs1997618
1587 K → R: in dbSNP:rs2230808
1611 N → D: in FHA1; deficient cellular cholesterol efflux
1615 R → Q: in dbSNP:rs1251839800
1648 L → P: in dbSNP:rs1883024
1670 A → T: in dbSNP:rs1203589782
1680 R → Q: in dbSNP:rs150125857
1704 V → D: in TGD; the mutant protein is retained in the endoplasmic reticulum while the wild-type protein is located at the plasma membrane
1731 S → C: in dbSNP:rs760507032
1897 R → W: in FHA1; uncertain significance; dbSNP:rs760768125
1925 R → Q: in Scott syndrome; shows impaired trafficking of the mutant protein to the plasma membrane; dbSNP:rs142688906
1952 K→M: Inhibits ATPase activity; when associated with M-939. Decreases translocase activity; when associated with M-939. Does not affect protein subcellular localization in plasma membrane and endosome; when associated with M-939.
2054 modified: Phosphoserine; by PKA
2081 R → W: in TGD; highly decreased protein abundance; highly decreased ATPase activity; highly decreased phospholipid translocase activity; loss protein subcellular localization to the plasma membrane; dbSNP:rs137854501
2109 A → T: in a colorectal cancer sample; somatic mutation
2150 P → L: in FHA1; moderately decreased protein abundance; does not affect ATPase activity; moderately decreased phospholipid translocase activity; dbSNP:rs369098049
2163 natural variant: F -> S
2168 L → P: in dbSNP:rs2853577
2243 D → E: in dbSNP:rs34879708
2244 V → I: in dbSNP:rs144588452

7tbyA The structure of human abca1 in nanodisc (see paper)
34% identity, 97% coverage: 5:306/311 of query aligns to 691:985/1788 of 7tbyA

query
sites
7tbyA

L

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P

P

T

T

A

A

G

G

V

V

D

D

V

P

E

Y

L

S

R

R

D

G

M

I

W

W

A

E

L

L

V

L

H

L

R

K

L

Y

R

R

E

Q

G

G

G

R

V

-

T

T

I

I

L

L

T

S

T

T

H

H

Y

H

I

M

E

D

E

E

A

A

E

D

E

V

M

L

A

G

D

D

R

R

V

I

G

A

V

I

I

I

S

S

K

H

G

G

E

K

L

L

I

C

L

C

V

V

E

G

E

S

K

S

T

L

A

F

L

L

M

K

K

N

K

Q

L

L

G

G

R

T

-

G

K

Y

T

Y

L

L

T

T

L

L

N

V

L

K

A

V

D

S

A

A

M

I

E

S

T

N

I

L

P

I

A

R

E

K

L

H

G

V

E

S

W

E

A

A

L

R

T

L

L

V

K

E

A

D

D

I

G

G

N

H

E

E

L

L

E

T

Y

Y

E

V

F

L

D

P

A

Y

N

E

A

G

E

A

R

F

T

V

G

E

V

L

-

F

P

H

S

E

L

I

L

D

K

D

R

R

M

L

S

S

D

D

I

L

G

G

V

I

G

S

F

-

K

-

D

S

L

Y

N

G

T

I

R

S

Q

E

S

T

L

L

E

E

I

I

F

F

V

L

N

K

L

V

V

A

H

W

K

K

Sites not aligning to the query:

binding cholesterol: 161, 162

7lkzA Structure of atp-bound human abca4 (see paper)
31% identity, 97% coverage: 3:303/311 of query aligns to 707:1022/1920 of 7lkzA

query
sites
7lkzA

P

P

V

G

L

V

S

C

I

V

A

K

S

N

L

L

S

V

K

K

T

I

Y
x
F

-

E

A

P

S

C

G

G

L

R

T

P

A
|
A

L

V

N

D

D

R

V

L

S

N

L

I

D

T

I

F

R

Y

K

E

G

N

E

Q

I

I

F

T

A

A

L

F

L

L

G

G

P

H

N
|
N

G
|
G

A

A

G
|
G

K
|
K

T
|
T

T

T

L

T

I

L

S

S

I

I

V

L

C

T

G

G

I

L

V

L

T

P

P

P

T

T

G

S

G

G

T

T

V

V

L

L

V

V

D

G

G

G

V

R

D

D

A

I

A

E

R

T

D

S

F

L

R

D

M

A

A

V

R

R

T

Q

R

S

I

L

G

G

L

M

V

C

P

P

Q
|
Q

E

-

L

-

H

H

T

N

D

I

S

L

F

F

E

H

-

H

-

L

T

T

V

V

W

A

A

E

T

H

V

M

T

L

F

F

S

Y

R

A

G

Q

L

L

F

K

G

G

K

K

P

S

P

Q

S

E

P

E

A

A

H

Q

V

L

E

E

-

M

-

E

K

A

V

M

L

L

K

E

D

D

L

T

S

G

L

L

W

H

D

H

K
|
K

R

R

K

N

S

E

K

E

I

A

Q

Q

E
x
D

L

L

S
|
S

G

G

M

M

K

Q

R

R

R

K

V

L

M

S

I

V

A

A

K

I

A

A

L

F

S

V

H

G

E

D

P

A

D

K

I

V

L

V

F

I

L

L

D

D

E

Q

P

P

T

T

A

S

G

G

V

V

D

D

V

P

E

Y

L

S

R

R

D

S

M

I

W

W

A

D

L

L

V

L

H

L

R

K

L

Y

R

R

E

S

G

G

G

R

V

-

T

T

I

I

L

M

T

S

T

T

H

H

Y

H

I

M

E

D

E

E

A

A

E

D

E

L

M

L

A

G

D

D

R

R

V

I

G

A

V

I

I

I

S

A

K

Q

G

G

E

R

L

L

I

Y

L

C

V

S

E

G

E

T

K

P

T

L

A

F

L

L

M

K

K

N

K

C

L

F

G

G

-

T

-

G

-

L

-

Y

-

L

-

T

-

L

-

V

-

R

-

K

-

M

R

K

K

Q

T

V

L

L

T

D

L

G

N

D

L

V

A

N

D

E

A

L

M

M

E

D

T

V

I

V

P

L

A

H

E

H

L

V

G

P

E

E

W

-

A

A

L

K

T

L

L

V

K

E

A

C

D

I

G

G

N

Q

E

E

L

L

E

I

Y

F

E

L

F

L

-

P

D

N

A

K

N

N

A

F

E

K

R

H

T

R

G

A

V

Y

P

A

S

S

L

L

L

F

K

R

R

E

M

L

S

E

D

E

I

T

-

L

-

A

G

D

V

L

G

G

F

L

K

S

D

S

L

F

N

G

T

I

R

S

Q

D

S

T

S

P

L

L

E

E

I

I

F

F

V

L

N

K

L

V

binding adenosine-5'-triphosphate: F718 (≠ Y14), A725 (= A20), N745 (= N40), G746 (= G41), G748 (= G43), K749 (= K44), T750 (= T45), K834 (= K127), D841 (≠ E134), S843 (= S136)
binding magnesium ion: T750 (= T45), Q790 (= Q85)

Sites not aligning to the query:

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: 22, 465, 566, 567, 568, 572, 592, 593, 595, 1076, 1355, 1362, 1470, 1471
binding adenosine-5'-triphosphate: 1619, 1646, 1647, 1649, 1650, 1651, 1652, 1742, 1744, 1745

Q5SSE9 ATP-binding cassette sub-family A member 13; EC 7.6.2.- from Mus musculus (Mouse) (see paper)
31% identity, 97% coverage: 3:305/311 of query aligns to 3808:4128/5034 of Q5SSE9

query
sites
Q5SSE9

P

P

V

G

L

V

S

A

I

L

A

I

S

S

L

V

S

T

K

K

T

E

Y

Y

A

E

S

D

G

H

L

K

T

V

A

A

L

V

N

Q

D

E

V

L

S

T

L

L

D

T

I

F

R

H

K

R

G

D

E

Q

I

I

F

T

A

A

L

L

G

G

P

T

N

N

G

G

A

A

G

G

K
|
K

T

T

L

I

I

I

S

S

I

M

V

L

C

M

G

G

I

L

V

F

T

P

P

P

T

T

G

S

G

G

T

T

V

I

L

T

V

I

D

N

G

G

V

K

D

N

A

L

A

Q

R

T

D

D

F

L

R

S

M

K

A

V

R

R

T

E

R

E

I

L

G

G

L

V

V

C

P

P

Q

Q

E

Q

-

D

L

V

H

L

T

L

D

D

S

N

F

L

E

-

T

-

V

-

W

-

A

-

T

T

V

V

T

R

F

E

S

H

R

L

G

M

L

L

F

F

G

A

K

S

P

I

P

K

S

A

P

P

-

W

-

T

-

K

-

E

-

L

-

Q

A

Q

H

Q

V

V

E

N

K

K

V

T

L

L

K

D

D

E

L

V

S

E

L

L

W

T

D

Q

K

H

R

Q

K

H

S

K

K

P

I

A

Q

G

E

V

L

L

S

S

G

G

M

M

K

K

R

R

R

K

V

L

M

S

I

I

A

G

K

I

A

A

L

F

S

M

H

G

E

M

P

S

D

K

I

T

L

V

F

V

L

L

D

D

E

E

P

P

T

S

A

S

G

G

V

V

D

D

V

P

E

C

L

S

R

R

D

S

M

L

W

W

A

D

L

I

V

L

H

L

R

K

L

Y

R

R

E

E

G

G

G

R

V

-

T

T

I

I

L

F

T

T

T
|
T

H

H

Y

H

I

L

E

D

E

E

A

A

E

E

E

M

M

L

A

S

D

D

R

H

V

V

G

A

V

V

I

L

S

Q

K

Q

G

G

E

R

L

L

I

R

L

C

V

Y

E

A

E

P

K

P

T

A

A

D

L

L

M

K

K

E

K

T

L

Y

G

G

R

-

K

Q

T

G

L

L

T

T

L

L

N

T

L

L

A

S

D

K

A

Q

-

P

-

S

-

I

M

L

E

E

T

T

I

Q

-

E

P

P

A

K

E

D

L

V

G

A

E

R

-

V

-

T

-

S

-

L

-

I

-

Q

-

I

-

Y

-

I

-

P

W

Q

A

A

L

F

T

L

L

K

K

D

A

S

D

C

G

G

N

G

E

E

L

L

E

T

Y

Y

E

T

F

I

D

P

A

K

N

D

A

A

E

D

R

R

T

T

G

C

V

F

P

K

S

G

L

L

-

C

-

Q

-

A

-

L

-

D

-

Q

-

N

L

L

K

Q

R

H

M

L

S

H

D

L

I

T

G

G

V

Y

G

G

F

I

K

S

D

D

L

-

N

-

T

-

R

-

Q

-

S

T

L

L

E

E

I

V

F

F

V

L

N

M

L

L

V

L

H

Q

K3849 (= K44) mutation to M: Does not affect intracellular vesicle localization. Affects cholesterol internalization.
T3999 (= T191) mutation to A: Does not affect protein expression. Affects intracellular vesicles localization. Impairs intracellular cholesterol accumulation in the vesicle.

Sites not aligning to the query:

3577 H→P: Does not affect protein expression. Decreases intracellular cholesterol accumulation in the vesicle.
4735 K→M: Does not affect intracellular vesicle localization.Affects cholesterol internalization.
4818 R→C: Does not affect protein expression. Decreases intracellular cholesterol accumulation in the vesicle.

P78363 Retinal-specific phospholipid-transporting ATPase ABCA4; ATP-binding cassette sub-family A member 4; RIM ABC transporter; RIM proteinv; RmP; Retinal-specific ATP-binding cassette transporter; Stargardt disease protein; EC 7.6.2.1 from Homo sapiens (Human) (see 43 papers)
36% identity, 78% coverage: 3:244/311 of query aligns to 927:1169/2273 of P78363

query
sites
P78363

P
|
P

V
x
G

L
x
V

S
x
C

I
x
V

A
x
K

S
x
N

L
|
L

S
x
V

K
|
K

T
x
I

Y
x
F

-
x
E

A
x
P

S
x
C

G
|
G

L
x
R

T
x
P

A
|
A

L
x
V

N
x
D

D
x
R

V
x
L

S
x
N

L
x
I

D
x
T

I
x
F

R
x
Y

K
x
E

G
x
N

E
x
Q

I
|
I

F
x
T

A
|
A

L
x
F

L
|
L

G
|
G

P
x
H

N
|
N

G
|
G

A
|
A

G
|
G

K
|
K

T
|
T

L
x
T

I
x
L

S
|
S

I
|
I

V
x
L

C
x
T

G
|
G

I
x
L

V
x
L

T
x
P

P
|
P

T
|
T

G
x
S

G
|
G

T
|
T

V
|
V

L
|
L

V
|
V

D
x
G