SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_085544283.1 NCBI__GCF_900177735.1:WP_085544283.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

6yu7A Crystal structure of mhst in complex with l-tyrosine (see paper)
45% identity, 98% coverage: 7:445/446 of query aligns to 2:442/442 of 6yu7A

query
sites
6yu7A

R

R

G

E

E

Q

W

W

S

A

S

S

K

R

I

L

G

G

F

F

I

I

L

L

A

A

A

A

A

M

G

G

S
|
S

A
|
A

I

V

G
|
G

L

L

G
|
G

S

N

I

I

W

W

R

R

F

F

P

S

Y

Y

I

V

T

T

G

G

Q

E

S

N

G

G

G

G

A

A

V

A

F

F

V

L

L

L

V

V

Y

Y

L

L

F

G

L

F

V

I

F

A

T

L

I

I

G

G

I

I

S

P

L

I

M

V

M

L

A

A

E

E

I

F

V

T

I

I

G

G

K

R

K

R

G

A

R

Q

L

S

N

D

A

A

V

V

G

G

S

S

F

F

R

E

R

K

L

L

G

A

-

P

G

G

K

K

H

P

W

W

G

K

L

V

V

A

G

G

W

L

I

M

G

G

V

V

I

A

A

A

A

G

F

F

V

L

I

I

L

L

S

S

Y

F

Y
|
Y

G

G

V

V

I

I

G

A

G

G

W

W

T

I

M

L

A

F

Y

Y

V

L

L

F

K

N

S

Y

F

I

T

T

G

G

L

Q

I

L

S

W

T

S

S

A

G

P

S

A

G

E

E

G

V

F

A

G

K

G

I

F

F

F

E

E

A

G

F

F

I

I

S

A

D

N

G

P

K

T

Q

L

M

P

L

L

F

F

Y

W

Q

Q

G

A

L

L

F

F

M

M

A

I

A

A

T

T

V

I

I

W

V

I

V

V

F

A

R

I

G

G

V

V

S

K

G

K

G

G

I

I

E

E

R

R

L

S

C

N

T

K

F

I

C

L

M

M

P

P

A

L

L

L

F

G

I

V

L

L

M

L

I

I

L

A

L

L

I

A

G

I

R

Y

A

S

L

L

T

T

L

L

P

G

G

G

A

A

M

K

E

E

G

G

V

L

V

A

F

F

F

L

L

F

K

S

P

P

D

D

F

W

S

S

K

A

L

L

S

K

G

D

E

P

-

G

V

V

V

Y

L

L

S

A

A

A

M

I

G

S

Q

Q

A

A

F

F

F
|
F

S
x
T

L

L

S
|
S

L

L

G

G

I
x
M

G

G

A

A

M

L

V

I

I

T

Y

Y

G

G

S

S

Y

Y

L

V

P

S

D

K

K

D

T

S

D

R

I

L

P

P

K

G

A

A

S

A

L

V

Q

S

I

V

C

A

F

G

L

L

T

D

F

T

M

A

I

F

S

A

L

I

M

I

A

A

G

G

L

I

I

M

I

I

F

F

P

P

S

A

L

V

F

F

A

A

F

L

G

G

M

L

E

S

P

P

G

S

A

G

G

G

A

P

G

G

L

L

T

V

F

F

I

V

T

V

L

L

P

P

V

D

V

I

F

F

A

D

K

S

M

I

P

R

G

L

G

G

V

P

L

I

W

V

A

G

A

I

S

A

F

F

F

F

V

I

L

L

F

L

F

G

F

A

A

A

A

A

L

L

T

S

S

S

S

A

V

V

S

S

L
|
L

L

L

E

E

V

V

V

P

V

V

S

A

Y

Y

M

F

I

M

D

R

S

K

L

F

G

D

W

W

D

S

R

R

K

K

K

Q

A

A

T

A

I

I

L

T

L

L

G

G

V

V

L

I

I

I

S

T

V

L

V

L

G

G

I

I

P

P

S

S

A

S

L

L

S

S

-

F

-

G

-

V

Q

L

G

G

A

E

V

V

T

T

I

I

N

-

V

I

F

P

G

G

V

L

S

N

F

I

L

F

D

D

A

S

A

V

D

D

F

F

F

I

A

A

S

S

N

S

L

V

L

F

M

L

P

P

A

L

G

G

G

G

F

M

L

I

T

I

A

A

V

L

F

F

I

I

G

G

W

W

V

G

A

W

Y

K

S

T

A

S

G

-

F

-

D

D

G

A

I

L

T

A

G

E

G

S

G

D

A

L

V

T

P

D

F

S

R

V

H

W

K

G

K

K

L

L

W

W

M

I

F

L

I

S

L

L

R

R

F

F

I

I

A

A

P

P

V

I

C

A

I

I

L

L

V

I

I

V

F

F

I

L

A

S

G

A

L

F

K

Q

binding tyrosine: S18 (= S23), A19 (= A24), G21 (= G26), G23 (= G28), Y101 (= Y105), F223 (= F226), T224 (≠ S227), S226 (= S229), M229 (≠ I232), L321 (= L324)

6yu4A Crystal structure of mhst in complex with l-4f-phenylalanine (see paper)
45% identity, 98% coverage: 7:445/446 of query aligns to 2:442/442 of 6yu4A

query
sites
6yu4A

R

R

G

E

E

Q

W

W

S

A

S

S

K

R

I

L

G

G

F

F

I

I

L

L

A

A

A

A

A

M

G

G

S
|
S

A
|
A

I

V

G
|
G

L
|
L

G
|
G

S

N

I

I

W

W

R

R

F

F

P

S

Y

Y

I

V

T

T

G

G

Q

E

S

N

G

G

G

G

A

A

V

A

F

F

V

L

L

L

V

V

Y

Y

L

L

F

G

L

F

V

I

F

A

T

L

I

I

G

G

I

I

S

P

L

I

M

V

M

L

A

A

E

E

I

F

V

T

I

I

G

G

K

R

K

R

G

A

R

Q

L

S

N

D

A

A

V

V

G

G

S

S

F

F

R

E

R

K

L

L

G

A

-

P

G

G

K

K

H

P

W

W

G

K

L

V

V

A

G

G

W

L

I

M

G

G

V

V

I

A

A

A

A

G

F

F

V

L

I

I

L

L

S

S

Y

F

Y
|
Y

G

G

V

V

I

I

G

A

G

G

W

W

T

I

M

L

A

F

Y

Y

V

L

L

F

K

N

S

Y

F

I

T

T

G

G

L

Q

I

L

S

W

T

S

S

A

G

P

S

A

G

E

E

G

V

F

A

G

K

G

I

F

F

F

E

E

A

G

F

F

I

I

S

A

D

N

G

P

K

T

Q

L

M

P

L

L

F

F

Y

W

Q

Q

G

A

L

L

F

F

M

M

A

I

A

A

T

T

V

I

I

W

V

I

V

V

F

A

R

I

G

G

V

V

S

K

G

K

G

G

I

I

E

E

R

R

L

S

C

N

T

K

F

I

C

L

M

M

P

P

A

L

L

L

F

G

I

V

L

L

M

L

I

I

L

A

L

L

I

A

G

I

R

Y

A

S

L

L

T

T

L

L

P

G

G

G

A

A

M

K

E

E

G

G

V

L

V

A

F

F

F

L

L

F

K

S

P

P

D

D

F

W

S

S

K

A

L

L

S

K

G

D

E

P

-

G

V

V

V

Y

L

L

S

A

A

A

M

I

G

S

Q

Q

A

A

F

F

F
|
F

S
x
T

L

L

S
|
S

L

L

G

G

I
x
M

G

G

A

A

M

L

V

I

I

T

Y

Y

G

G

S

S

Y

Y

L

V

P

S

D

K

K

D

T

S

D

R

I

L

P

P

K

G

A

A

S

A

L

V

Q

S

I

V

C

A

F

G

L

L

T

D

F

T

M

A

I

F

S

A

L

I

M

I

A

A

G

G

L

I

I

M

I

I

F

F

P

P

S

A

L

V

F

F

A

A

F

L

G

G

M

L

E

S

P

P

G

S

A

G

G

G

A

P

G

G

L

L

T

V

F

F

I

V

T

V

L

L

P

P

V

D

V

I

F

F

A

D

K

S

M

I

P

R

G

L

G

G

V

P

L

I

W

V

A

G

A

I

S

A

F

F

F

F

V

I

L

L

F

L

F

G

F

A

A

A

A

A

L

L

T

S

S

S

S

A

V

V

S

S

L
|
L

L

L

E

E

V

V

V

P

V

V

S

A

Y

Y

M

F

I

M

D

R

S

K

L

F

G

D

W

W

D

S

R

R

K

K

K

Q

A

A

T

A

I

I

L

T

L

L

G

G

V

V

L

I

I

I

S

T

V

L

V

L

G

G

I

I

P

P

S

S

A

S

L

L

S

S

-

F

-

G

-

V

Q

L

G

G

A

E

V

V

T

T

I

I

N

-

V

I

F

P

G

G

V

L

S

N

F

I

L

F

D

D

A

S

A

V

D

D

F

F

F

I

A

A

S

S

N

S

L

V

L

F

M

L

P

P

A

L

G

G

G

G

F

M

L

I

T

I

A

A

V

L

F

F

I

I

G

G

W

W

V

G

A

W

Y

K

S

T

A

S

G

-

F

-

D

D

G

A

I

L

T

A

G

E

G

S

G

D

A

L

V

T

P

D

F

S

R

V

H

W

K

G

K

K

L

L

W

W

M

I

F

L

I

S

L

L

R

R

F

F

I

I

A

A

P

P

V

I

C

A

I

I

L

L

V

I

I

V

F

F

I

L

A

S

G

A

L

F

K

Q

binding 4-fluoro-l-phenylalanine: S18 (= S23), A19 (= A24), G21 (= G26), L22 (= L27), G23 (= G28), Y101 (= Y105), F223 (= F226), T224 (≠ S227), S226 (= S229), M229 (≠ I232), L321 (= L324)

6yu6B Crystal structure of mhst in complex with l-leucine (see paper)
45% identity, 98% coverage: 7:445/446 of query aligns to 2:442/446 of 6yu6B

query
sites
6yu6B

R

R

G

E

E

Q

W

W

S

A

S

S

K

R

I

L

G

G

F

F

I

I

L

L

A

A

A

A

A

M

G

G

S
|
S

A
|
A

I

V

G
|
G

L

L

G
|
G

S

N

I

I

W

W

R

R

F

F

P

S

Y

Y

I

V

T

T

G

G

Q

E

S

N

G

G

G

G

A

A

V

A

F

F

V

L

L

L

V

V

Y

Y

L

L

F

G

L

F

V

I

F

A

T

L

I

I

G

G

I

I

S

P

L

I

M

V

M

L

A

A

E

E

I

F

V

T

I

I

G

G

K

R

K

R

G

A

R

Q

L

S

N

D

A

A

V

V

G

G

S

S

F

F

R

E

R

K

L

L

G

A

-

P

G

G

K

K

H

P

W

W

G

K

L

V

V

A

G

G

W

L

I

M

G

G

V

V

I

A

A

A

A

G

F

F

V

L

I

I

L

L

S

S

Y

F

Y

Y

G

G

V

V

I

I

G

A

G

G

W

W

T

I

M

L

A

F

Y

Y

V

L

L

F

K

N

S

Y

F

I

T

T

G

G

L

Q

I

L

S

W

T

S

S

A

G

P

S

A

G

E

E

G

V

F

A

G

K

G

I

F

F

F

E

E

A

G

F

F

I

I

S

A

D

N

G

P

K

T

Q

L

M

P

L

L

F

F

Y

W

Q

Q

G

A

L

L

F

F

M

M

A

I

A

A

T

T

V

I

I

W

V

I

V

V

F

A

R

I

G

G

V

V

S

K

G

K

G

G

I

I

E

E

R

R

L

S

C

N

T

K

F

I

C

L

M

M

P

P

A

L

L

L

F

G

I

V

L

L

M

L

I

I

L

A

L

L

I

A

G

I

R

Y

A

S

L

L

T

T

L

L

P

G

G

G

A

A

M

K

E

E

G

G

V

L

V

A

F

F

F

L

L

F

K

S

P

P

D

D

F

W

S

S

K

A

L

L

S

K

G

D

E

P

-

G

V

V

V

Y

L

L

S

A

A

A

M

I

G

S

Q

Q

A

A

F

F

F
|
F

S
x
T

L

L

S
|
S

L

L

G

G

I
x
M

G

G

A

A

M

L

V

I

I

T

Y

Y

G

G

S

S

Y

Y

L

V

P

S

D

K

K

D

T

S

D

R

I

L

P

P

K

G

A

A

S

A

L

V

Q

S

I

V

C

A

F

G

L

L

T

D

F

T

M

A

I

F

S

A

L

I

M

I

A

A

G

G

L

I

I

M

I

I

F

F

P

P

S

A

L

V

F

F

A

A

F

L

G

G

M

L

E

S

P

P

G

S

A

G

G

G

A

P

G

G

L

L

T

V

F

F

I

V

T

V

L

L

P

P

V

D

V

I

F

F

A

D

K

S

M

I

P

R

G

L

G

G

V

P

L

I

W

V

A

G

A

I

S

A

F

F

F

F

V

I

L

L

F

L

F

G

F

A

A

A

A

A

L

L

T

S

S

S

S

A

V

V

S

S

L
|
L

L

L

E

E

V

V

V

P

V

V

S

A

Y

Y

M

F

I

M

D

R

S

K

L

F

G

D

W

W

D

S

R

R

K

K

K

Q

A

A

T

A

I

I

L

T

L

L

G

G

V

V

L

I

I

I

S

T

V

L

V

L

G

G

I

I

P

P

S

S

A

S

L

L

S

S

-

F

-

G

-

V

Q

L

G

G

A

E

V

V

T

T

I

I

N

-

V

I

F

P

G

G

V

L

S

N

F

I

L

F

D

D

A

S

A

V

D

D

F

F

F

I

A

A

S

S

N

S

L

V

L

F

M

L

P

P

A

L

G

G

G

G

F

M

L

I

T

I

A

A

V

L

F

F

I

I

G

G

W

W

V

G

A

W

Y

K

S

T

A

S

G

-

F

-

D

D

G

A

I

L

T

A

G

E

G

S

G

D

A

L

V

T

P

D

F

S

R

V

H

W

K

G

K

K

L

L

W

W

M

I

F

L

I

S

L

L

R

R

F

F

I

I

A

A

P

P

V

I

C

A

I

I

L

L

V

I

I

V

F

F

I

L

A

S

G

A

L

F

K

Q

binding leucine: S18 (= S23), A19 (= A24), G21 (= G26), G23 (= G28), F223 (= F226), T224 (≠ S227), S226 (= S229), M229 (≠ I232), L321 (= L324)

6yu5A Crystal structure of mhst in complex with l-valine (see paper)
45% identity, 98% coverage: 7:442/446 of query aligns to 2:439/441 of 6yu5A

query
sites
6yu5A

R

R

G

E

E

Q

W

W

S

A

S

S

K

R

I

L

G

G

F

F

I

I

L

L

A

A

A

A

A

M

G

G

S
|
S

A
|
A

I

V

G

G

L

L

G
|
G

S

N

I

I

W

W

R

R

F

F

P

S

Y

Y

I

V

T

T

G

G

Q

E

S

N

G

G

G

G

A

A

V

A

F

F

V

L

L

L

V

V

Y

Y

L

L

F

G

L

F

V

I

F

A

T

L

I

I

G

G

I

I

S

P

L

I

M

V

M

L

A

A

E

E

I

F

V

T

I

I

G

G

K

R

K

R

G

A

R

Q

L

S

N

D

A

A

V

V

G

G

S

S

F

F

R

E

R

K

L

L

G

A

-

P

G

G

K

K

H

P

W

W

G

K

L

V

V

A

G

G

W

L

I

M

G

G

V

V

I

A

A

A

A

G

F

F

V

L

I

I

L

L

S

S

Y

F

Y
|
Y

G

G

V

V

I

I

G

A

G

G

W

W

T

I

M

L

A

F

Y

Y

V

L

L

F

K

N

S

Y

F

I

T

T

G

G

L

Q

I

L

S

W

T

S

S

A

G

P

S

A

G

E

E

G

V

F

A

G

K

G

I

F

F

F

E

E

A

G

F

F

I

I

S

A

D

N

G

P

K

T

Q

L

M

P

L

L

F

F

Y

W

Q

Q

G

A

L

L

F

F

M

M

A

I

A

A

T

T

V

I

I

W

V

I

V

V

F

A

R

I

G

G

V

V

S

K

G

K

G

G

I

I

E

E

R

R

L

S

C

N

T

K

F

I

C

L

M

M

P

P

A

L

L

L

F

G

I

V

L

L

M

L

I

I

L

A

L

L

I

A

G

I

R

Y

A

S

L

L

T

T

L

L

P

G

G

G

A

A

M

K

E

E

G

G

V

L

V

A

F

F

F

L

L

F

K

S

P

P

D

D

F

W

S

S

K

A

L

L

S

K

G

D

E

P

-

G

V

V

V

Y

L

L

S

A

A

A

M

I

G

S

Q

Q

A

A

F

F

F
|
F

S
x
T

L

L

S
|
S

L

L

G

G

I

M

G

G

A

A

M

L

V

I

I

T

Y

Y

G

G

S

S

Y

Y

L

V

P

S

D

K

K

D

T

S

D

R

I

L

P

P

K

G

A

A

S

A

L

V

Q

S

I

V

C

A

F

G

L

L

T

D

F

T

M

A

I

F

S

A

L

I

M

I

A

A

G

G

L

I

I

M

I

I

F

F

P

P

S

A

L

V

F

F

A

A

F

L

G

G

M

L

E

S

P

P

G

S

A

G

G

G

A

P

G

G

L

L

T

V

F

F

I

V

T

V

L

L

P

P

V

D

V

I

F

F

A

D

K

S

M

I

P

R

G

L

G

G

V

P

L

I

W

V

A

G

A

I

S

A

F

F

F

F

V

I

L

L

F

L

F

G

F

A

A

A

A

A

L

L

T

S

S
|
S

S

A

V

V

S

S

L

L

L

L

E

E

V

V

V

P

V

V

S

A

Y

Y

M

F

I

M

D

R

S

K

L

F

G

D

W

W

D

S

R

R

K

K

K

Q

A

A

T

A

I

I

L

T

L

L

G

G

V

V

L

I

I

I

S

T

V

L

V

L

G

G

I

I

P

P

S

S

A

S

L

L

S

S

-

F

-

G

-

V

Q

L

G

G

A

E

V

V

T

T

I

I

N

-

V

I

F

P

G

G

V

L

S

N

F

I

L

F

D

D

A

S

A

V

D

D

F

F

F

I

A

A

S

S

N

S

L

V

L

F

M

L

P

P

A

L

G

G

G

G

F

M

L

I

T

I

A

A

V

L

F

F

I

I

G

G

W

W

V

G

A

W

Y

K

S

T

A

S

G

-

F

-

D

D

G

A

I

L

T

A

G

E

G

S

G

D

A

L

V

T

P

D

F

S

R

V

H

W

K

G

K

K

L

L

W

W

M

I

F

L

I

S

L

L

R

R

F

F

I

I

A

A

P

P

V

I

C

A

I

I

L

L

V

I

I

V

F

F

I

L

A

S

binding valine: S18 (= S23), A19 (= A24), G23 (= G28), Y101 (= Y105), F223 (= F226), T224 (≠ S227), S226 (= S229), S317 (= S320)

6yu3A Crystal structure of mhst in complex with l-phenylalanine (see paper)
45% identity, 98% coverage: 7:442/446 of query aligns to 2:439/441 of 6yu3A

query
sites
6yu3A

R

R

G

E

E

Q

W

W

S

A

S

S

K

R

I

L

G

G

F

F

I

I

L

L

A

A

A

A

A

M

G

G

S
|
S

A
|
A

I

V

G
|
G

L

L

G
|
G

S

N

I

I

W

W

R

R

F

F

P

S

Y

Y

I

V

T

T

G

G

Q

E

S

N

G

G

G

G

A

A

V

A

F

F

V

L

L

L

V

V

Y

Y

L

L

F

G

L

F

V

I

F

A

T

L

I

I

G

G

I

I

S

P

L

I

M

V

M

L

A

A

E

E

I

F

V

T

I

I

G

G

K

R

K

R

G

A

R

Q

L

S

N

D

A

A

V

V

G

G

S

S

F

F

R

E

R

K

L

L

G

A

-

P

G

G

K

K

H

P

W

W

G

K

L

V

V

A

G

G

W

L

I

M

G

G

V

V

I

A

A

A

A

G

F

F

V

L

I

I

L

L

S

S

Y

F

Y
|
Y

G

G

V

V

I

I

G

A

G

G

W

W

T

I

M

L

A

F

Y

Y

V

L

L

F

K

N

S

Y

F

I

T

T

G

G

L

Q

I

L

S

W

T

S

S

A

G

P

S

A

G

E

E

G

V

F

A

G

K

G

I

F

F

F

E

E

A

G

F

F

I

I

S

A

D

N

G

P

K

T

Q

L

M

P

L

L

F

F

Y

W

Q

Q

G

A

L

L

F

F

M

M

A

I

A

A

T

T

V

I

I

W

V

I

V

V

F

A

R

I

G

G

V

V

S

K

G

K

G

G

I

I

E

E

R

R

L

S

C

N

T

K

F

I

C

L

M

M

P

P

A

L

L

L

F

G

I

V

L

L

M

L

I

I

L

A

L

L

I

A

G

I

R

Y

A

S

L

L

T

T

L

L

P

G

G

G

A

A

M

K

E

E

G

G

V

L

V

A

F

F

F

L

L

F

K

S

P

P

D

D

F

W

S

S

K

A

L

L

S

K

G

D

E

P

-

G

V

V

V

Y

L

L

S

A

A

A

M

I

G

S

Q

Q

A

A

F

F

F
|
F

S
x
T

L

L

S
|
S

L

L

G

G

I
x
M

G

G

A

A

M

L

V

I

I

T

Y

Y

G

G

S

S

Y

Y

L

V

P

S

D

K

K

D

T

S

D

R

I

L

P

P

K

G

A

A

S

A

L

V

Q

S

I

V

C

A

F

G

L

L

T

D

F

T

M

A

I

F

S

A

L

I

M

I

A

A

G

G

L

I

I

M

I

I

F

F

P

P

S

A

L

V

F

F

A

A

F

L

G

G

M

L

E

S

P

P

G

S

A

G

G

G

A

P

G

G

L

L

T

V

F

F

I

V

T

V

L

L

P

P

V

D

V

I

F

F

A

D

K

S

M

I

P

R

G

L

G

G

V

P

L

I

W

V

A

G

A

I

S

A

F

F

F

F

V

I

L

L

F

L

F

G

F

A

A

A

A

A

L

L

T

S

S

S

S

A

V

V

S

S

L
|
L

L

L

E

E

V

V

V

P

V

V

S

A

Y

Y

M

F

I

M

D

R

S

K

L

F

G

D

W

W

D

S

R

R

K

K

K

Q

A

A

T

A

I

I

L

T

L

L

G

G

V

V

L

I

I

I

S

T

V

L

V

L

G

G

I

I

P

P

S

S

A

S

L

L

S

S

-

F

-

G

-

V

Q

L

G

G

A

E

V

V

T

T

I

I

N

-

V

I

F

P

G

G

V

L

S

N

F

I

L

F

D

D

A

S

A

V

D

D

F

F

F

I

A

A

S

S

N

S

L

V

L

F

M

L

P

P

A

L

G

G

G

G

F

M

L

I

T

I

A

A

V

L

F

F

I

I

G

G

W

W

V

G

A

W

Y

K

S

T

A

S

G

-

F

-

D

D

G

A

I

L

T

A

G

E

G

S

G

D

A

L

V

T

P

D

F

S

R

V

H

W

K

G

K

K

L

L

W

W

M

I

F

L

I

S

L

L

R

R

F

F

I

I

A

A

P

P

V

I

C

A

I

I

L

L

V

I

I

V

F

F

I

L

A

S

binding phenylalanine: S18 (= S23), A19 (= A24), G21 (= G26), G23 (= G28), Y101 (= Y105), F223 (= F226), T224 (≠ S227), S226 (= S229), M229 (≠ I232), L321 (= L324)

6yu2A Crystal structure of mhst in complex with l-isoleucine (see paper)
45% identity, 98% coverage: 7:442/446 of query aligns to 2:439/441 of 6yu2A

query
sites
6yu2A

R

R

G

E

E

Q

W

W

S

A

S

S

K

R

I

L

G

G

F

F

I

I

L

L

A

A

A

A

A

M

G

G

S
|
S

A
|
A

I

V

G
|
G

L

L

G
|
G

S

N

I

I

W

W

R

R

F

F

P

S

Y

Y

I

V

T

T

G

G

Q

E

S

N

G

G

G

G

A

A

V

A

F

F

V

L

L

L

V

V

Y

Y

L

L

F

G

L

F

V

I

F

A

T

L

I

I

G

G

I

I

S

P

L

I

M

V

M

L

A

A

E

E

I

F

V

T

I

I

G

G

K

R

K

R

G

A

R

Q

L

S

N

D

A

A

V

V

G

G

S

S

F

F

R

E

R

K

L

L

G

A

-

P

G

G

K

K

H

P

W

W

G

K

L

V

V

A

G

G

W

L

I

M

G

G

V

V

I

A

A

A

A

G

F

F

V

L

I

I

L

L

S

S

Y

F

Y
|
Y

G

G

V

V

I

I

G

A

G

G

W

W

T

I

M

L

A

F

Y

Y

V

L

L

F

K

N

S

Y

F

I

T

T

G

G

L

Q

I

L

S

W

T

S

S

A

G

P

S

A

G

E

E

G

V

F

A

G

K

G

I

F

F

F

E

E

A

G

F

F

I

I

S

A

D

N

G

P

K

T

Q

L

M

P

L

L

F

F

Y

W

Q

Q

G

A

L

L

F

F

M

M

A

I

A

A

T

T

V

I

I

W

V

I

V

V

F

A

R

I

G

G

V

V

S

K

G

K

G

G

I

I

E

E

R

R

L

S

C

N

T

K

F

I

C

L

M

M

P

P

A

L

L

L

F

G

I

V

L

L

M

L

I

I

L

A

L

L

I

A

G

I

R

Y

A

S

L

L

T

T

L

L

P

G

G

G

A

A

M

K

E

E

G

G

V

L

V

A

F

F

F

L

L

F

K

S

P

P

D

D

F

W

S

S

K

A

L

L

S

K

G

D

E

P

-

G

V

V

V

Y

L

L

S

A

A

A

M

I

G

S

Q

Q

A

A

F

F

F
|
F

S

T

L

L

S

S

L

L

G

G

I
x
M

G

G

A

A

M

L

V

I

I

T

Y

Y

G

G

S

S

Y

Y

L

V

P

S

D

K

K

D

T

S

D

R

I

L

P

P

K

G

A

A

S

A

L

V

Q

S

I

V

C

A

F

G

L

L

T

D

F

T

M

A

I

F

S

A

L

I

M

I

A

A

G

G

L

I

I

M

I

I

F

F

P

P

S

A

L

V

F

F

A

A

F

L

G

G

M

L

E

S

P

P

G

S

A

G

G

G

A

P

G

G

L

L

T

V

F

F

I

V

T

V

L

L

P

P

V

D

V

I

F

F

A

D

K

S

M

I

P

R

G

L

G

G

V

P

L

I

W

V

A

G

A

I

S

A

F

F

F

F

V

I

L

L

F

L

F

G

F

A

A

A

A

A

L

L

T

S

S
|
S

S

A

V

V

S

S

L

L

L

L

E

E

V

V

V

P

V

V

S

A

Y

Y

M

F

I

M

D

R

S

K

L

F

G

D

W

W

D

S

R

R

K

K

K

Q

A

A

T

A

I

I

L

T

L

L

G

G

V

V

L

I

I

I

S

T

V

L

V

L

G

G

I

I

P

P

S

S

A

S

L

L

S

S

-

F

-

G

-

V

Q

L

G

G

A

E

V

V

T

T

I

I

N

-

V

I

F

P

G

G

V

L

S

N

F

I

L

F

D

D

A

S

A

V

D

D

F

F

F

I

A

A

S

S

N

S

L

V

L

F

M

L

P

P

A

L

G

G

G

G

F

M

L

I

T

I

A

A

V

L

F

F

I

I

G

G

W

W

V

G

A

W

Y

K

S

T

A

S

G

-

F

-

D

D

G

A

I

L

T

A

G

E

G

S

G

D

A

L

V

T

P

D

F

S

R

V

H

W

K

G

K

K

L

L

W

W

M

I

F

L

I

S

L

L

R

R

F

F

I

I

A

A

P

P

V

I

C

A

I

I

L

L

V

I

I

V

F

F

I

L

A

S

binding isoleucine: S18 (= S23), A19 (= A24), G21 (= G26), G23 (= G28), Y101 (= Y105), F223 (= F226), M229 (≠ I232), S317 (= S320)

4us3A Crystal structure of the bacterial nss member mhst in an occluded inward-facing state (see paper)
45% identity, 98% coverage: 7:442/446 of query aligns to 2:439/441 of 4us3A

query
sites
4us3A

R

R

G

E

E

Q

W

W

S

A

S

S

K

R

I

L

G

G

F

F

I

I

L

L

A

A

A

A

A

M

G
|
G

S
|
S

A
|
A

I
x
V

G
|
G

L
|
L

G
|
G

S
x
N

I

I

W

W

R

R

F

F

P

S

Y

Y

I

V

T

T

G

G

Q

E

S

N

G

G

G

G

A

A

V

A

F

F

V

L

L

L

V

V

Y

Y

L

L

F

G

L

F

V

I

F

A

T

L

I

I

G

G

I

I

S

P

L

I

M

V

M

L

A

A

E

E

I

F

V

T

I

I

G

G

K

R

K

R

G

A

R

Q

L

S

N

D

A

A

V

V

G

G

S

S

F

F

R

E

R

K

L

L

G

A

-

P

G

G

K

K

H

P

W

W

G

K

L

V

V

A

G

G

W

L

I

M

G

G

V

V

I

A

A

A

A

G

F

F

V

L

I

I

L

L

S

S

Y

F

Y
|
Y

G

G

V

V

I

I

G

A

G

G

W

W

T

I

M

L

A

F

Y

Y

V

L

L

F

K

N

S

Y

F

I

T

T

G

G

L

Q

I

L

S

W

T

S

S

A

G

P

S

A

G

E

E

G

V

F

A

G

K

G

I

F

F

F

E

E

A

G

F

F

I

I

S

A

D

N

G

P

K

T

Q

L

M

P

L

L

F

F

Y

W

Q

Q

G

A

L

L

F

F

M

M

A

I

A

A

T

T

V

I

I

W

V

I

V

V

F

A

R

I

G

G

V

V

S

K

G

K

G

G

I

I

E

E

R

R

L

S

C

N

T

K

F

I

C

L

M

M

P

P

A

L

L

L

F

G

I

V

L

L

M

L

I

I

L

A

L

L

I

A

G

I

R

Y

A

S

L

L

T

T

L

L

P

G

G

G

A

A

M

K

E

E

G

G

V

L

V

A

F

F

F

L

L

F

K

S

P

P

D

D

F

W

S

S

K

A

L

L

S

K

G

D

E

P

-

G

V

V

V

Y

L

L

S

A

A

A

M

I

G

S

Q

Q

A

A

F

F

F
|
F

S
x
T

L

L

S
|
S

L

L

G

G

I
x
M

G

G

A

A

M

L

V

I

I

T

Y

Y

G

G

S

S

Y

Y

L

V

P

S

D

K

K

D

T

S

D

R

I

L

P

P

K

G

A

A

S

A

L

V

Q

S

I

V

C

A

F

G

L

L

T
x
D

F

T

M

A

I

F

S

A

L

I

M

I

A

A

G

G

L

I

I

M

I

I

F

F

P

P

S

A

L

V

F

F

A

A

F

L

G

G

M

L

E

S

P

P

G

S

A

G

G

G

A

P

G

G

L

L

T

V

F

F

I

V

T

V

L

L

P

P

V

D

V

I

F

F

A

D

K

S

M

I

P

R

G

L

G

G

V

P

L

I

W

V

A

G

A

I

S

A

F

F

F

F

V

I

L

L

F

L

F

G

F

A

A
|
A

A

A

L

L

T
x
S

S
|
S

S

A

V

V

S
|
S

L
|
L

L

L

E

E

V

V

V

P

V

V

S

A

Y

Y

M

F

I

M

D

R

S

K

L

F

G

D

W

W

D

S

R

R

K

K

K

Q

A

A

T

A

I

I

L

T

L

L

G

G

V

V

L

I

I

I

S

T

V

L

V

L

G

G

I

I

P

P

S

S

A

S

L

L

S

S

-

F

-

G

-

V

Q

L

G

G

A

E

V

V

T

T

I

I

N

-

V

I

F

P

G

G

V

L

S

N

F

I

L

F

D

D

A

S

A

V

D

D

F

F

F

I

A

A

S

S

N

S

L

V

L

F

M

L

P

P

A

L

G

G

G

G

F

M

L

I

T

I

A

A

V

L

F

F

I

I

G

G

W

W

V

G

A

W

Y

K

S

T

A

S

G

-

F

-

D

D

G

A

I

L

T

A

G

E

G

S

G

D

A

L

V

T

P

D

F

S

R

V

H

W

K

G

K

K

L

L

W

W

M

I

F

L

I

S

L

L

R

R

F

F

I

I

A

A

P

P

V

I

C

A

I

I

L

L

V

I

I

V

F

F

I

L

A

S

binding sodium ion: G17 (= G22), A19 (= A24), V20 (≠ I25), V20 (≠ I25), G21 (= G26), N24 (≠ S29), T224 (≠ S227), D256 (≠ T259), A313 (= A316), S316 (≠ T319), S317 (= S320)
binding tryptophan: S18 (= S23), A19 (= A24), L22 (= L27), G23 (= G28), Y101 (= Y105), F223 (= F226), T224 (≠ S227), S226 (= S229), M229 (≠ I232), S320 (= S323), L321 (= L324)

4us4A Crystal structure of the bacterial nss member mhst in an occluded inward-facing state (lipidic cubic phase form) (see paper)
45% identity, 97% coverage: 15:445/446 of query aligns to 1:433/433 of 4us4A

query
sites
4us4A

G

G

F

F

I

I

L

L

A

A

A

A

A

M

G
|
G

S
|
S

A
|
A

I
x
V

G
|
G

L

L

G
|
G

S
x
N

I

I

W

W

R

R

F

F

P

S

Y

Y

I

V

T

T

G

G

Q

E

S

N

G

G

G

G

A

A

V

A

F

F

V

L

L

L

V

V

Y

Y

L

L

F

G

L

F

V

I

F

A

T

L

I

I

G

G

I

I

S

P

L

I

M

V

M

L

A

A

E

E

I

F

V

T

I

I

G

G

K

R

K

R

G

A

R

Q

L

S

N

D

A

A

V

V

G

G

S

S

F

F

R

E

R

K

L

L

G

A

-

P

G

G

K

K

H

P

W

W

G

K

L

V

V

A

G

G

W

L

I

M

G

G

V

V

I

A

A

A

A

G

F

F

V

L

I

I

L

L

S

S

Y

F

Y
|
Y

G

G

V

V

I

I

G

A

G

G

W

W

T

I

M

L

A

F

Y

Y

V

L

L

F

K

N

S

Y

F

I

T

T

G

G

L

Q

I

L

S

W

T

S

S

A

G

P

S

A

G

E

E

G

V

F

A

G

K

G

I

F

F

F

E

E

A

G

F

F

I

I

S

A

D

N

G

P

K

T

Q

L

M

P

L

L

F

F

Y

W

Q

Q

G

A

L

L

F

F

M

M

A

I

A

A

T

T

V

I

I

W

V

I

V

V

F

A

R

I

G

G

V

V

S

K

G

K

G

G

I

I

E

E

R

R

L

S

C

N

T

K

F

I

C

L

M

M

P

P

A

L

L

L

F

G

I

V

L

L

M

L

I

I

L
x
A

L

L

I

A

G

I

R

Y

A

S

L

L

T
|
T

L

L

P

G

G

G

A

A

M

K

E

E

G

G

V

L

V

A

F

F

F

L

L

F

K

S

P

P

D

D

F

W

S

S

K

A

L

L

S

K

G

D

E

P

-

G

V

V

V

Y

L

L

S

A

A

A

M

I

G

S

Q

Q

A

A

F

F

F
|
F

S
x
T

L

L

S
|
S

L

L

G

G

I
x
M

G

G

A

A

M

L

V

I

I

T

Y

Y

G

G

S

S

Y

Y

L

V

P

S

D

K

K

D

T

S

D

R

I

L

P

P

K

G

A

A

S

A

L

V

Q

S

I

V

C

A

F

G

L

L

T
x
D

F

T

M

A

I

F

S

A

L

I

M

I

A

A

G

G

L

I

I

M

I

I

F

F

P

P

S

A

L

V

F

F

A

A

F

L

G

G

M

L

E

S

P

P

G

S

A

G

G

G

A

P

G

G

L

L

T

V

F

F

I

V

T

V

L

L

P

P

V

D

V

I

F

F

A

D

K

S

M
x
I

P
x
R

G
x
L

G

G

V

P

L

I

W

V

A

G

A

I

S

A

F

F

F

F

V

I

L

L

F

L

F

G

F

A

A
|
A

A

A

L

L

T
x
S

S
|
S

S

A

V

V

S

S

L
|
L

L

L

E

E

V

V

V

P

V

V

S

A

Y

Y

M

F

I

M

D

R

S

K

L

F

G

D

W

W

D

S

R

R

K

K

K

Q

A

A

T

A

I

I

L

T

L

L

G

G

V

V

L

I

I

I

S

T

V

L

V

L

G

G

I

I

P

P

S

S

A

S

L

L

S

S

-

F

-

G

-

V

Q

L

G

G

A

E

V

V

T

T

I

I

N

-

V

I

F

P

G

G

V

L

S

N

F

I

L

F

D

D

A

S

A

V

D

D

F

F

F

I

A

A

S

S

N

S

L

V

L

F

M

L

P

P

A

L

G

G

G

G

F

M

L

I

T

I

A

A

V

L

F

F

I

I

G

G

W

W

V

G

A

W

Y

K

S

T

A

S

G

-

F

-

D

D

G

A

I

L

T

A

G

E

G

S

G

D

A

L

V

T

P

D

F

S

R

V

H

W

K

G

K

K

L

L

W

W

M

I

F

L

I

S

L

L

R

R

F

F

I

I

A

A

P

P

V

I

C

A

I

I

L

L

V

I

I

V

F

F

I

L

A

S

G

A

L

F

K

Q

binding (2s)-2,3-dihydroxypropyl(7z)-pentadec-7-enoate: A173 (≠ L186), T180 (= T193), I287 (≠ M299), R288 (≠ P300), L289 (≠ G301)
binding sodium ion: G8 (= G22), S9 (= S23), A10 (= A24), V11 (≠ I25), V11 (≠ I25), N15 (≠ S29), T215 (≠ S227), D247 (≠ T259), A304 (= A316), S307 (≠ T319), S308 (= S320)
binding tryptophan: S9 (= S23), A10 (= A24), G12 (= G26), G14 (= G28), Y92 (= Y105), F214 (= F226), T215 (≠ S227), S217 (= S229), M220 (≠ I232), L312 (= L324)

3gwwA Leucine transporter leut in complex with s-fluoxetine (see paper)
29% identity, 98% coverage: 7:441/446 of query aligns to 1:456/501 of 3gwwA

query
sites
3gwwA

R

R

G

E

E

H

W

W

S

A

S

T

K

R

I

L

G

G

F

L

I

I

L

L

A

A

A

M

A

A

G

G

S

N

A
|
A

I

V

G
|
G

L
|
L

G
|
G

S

N

I

F

W

L

R
|
R

F

F

P

P

Y

V

I

Q

T

A

G

A

Q

E

S

N

G

G

G

G

A

G

V

A

F

F

V

M

L

I

V

P

Y

Y

L

I

F

I

L

A

V

F

F

L

T

L

I

V

G

G

I

I

S

P

L

L

M

M

M

W

A

I

E

E

I

W

V

A

I

M

G

G

K

R

K

Y

G

G

R

G

L

A

N

Q

A

G

V

H

G

G

S

T

-

T

-

P

-

A

-

I

-

F

-

Y

-

L

-

L

F

W

R

R

R

N

L

R

G

F

G

A

K

K

H

I

W

L

G

G

L

V

V

F

G

G

-

L

W

W

I

I

G

P

V

L

I

V

A

V

A

A

F

I

V

-

I

-

L

-

S

-

Y

Y

Y
|
Y

G

V

V

Y

I

I

G

E

G

S

W

W

T

T

M

L

A

G

Y

F

V

A

L

I

K

K

S

F

F

L

T

V

G

G

L

L

I

V

S

P

T

E

S

P

G

P

S

P

G

D

E

S

V

I

A

L

K

R

I

P

F

F

E

K

A

E

F

F

-

L

-

Y

-

S

-

Y

-

I

-

G

I

V

S

P

D

K

G

G

K

D

Q

E

M

P

L

I

F

L

Y

K

Q

P

G

S

L

L

F

F

-

A

-

Y

-

I

-

V

-

F

-

L

-

I

-

T

M

M

A

F

A

I

T

N

V

V

I

S

V

I

V

L

F

I

R

R

G

G

V

I

S

S

G

K

G

G

I

I

E

E

R

R

L

F

C

A

T

K

F

I

C

A

M

M

P

P

A

T

L

L

F

F

I

I

L

L

M

A

I

V

L

F

L

L

I

V

G

I

R

R

A

V

-

F

-

L

L

L

T

E

L

T

P

P

G

N

-

G

-

T

A

A

M

A

E

D

G

G

V

L

V

N

F

F

F

L

L

W

K

T

P

P

D

D

F

F

S

E

K

K

L

L

S

K

G

D

E

P

-

G

V

V

V

W

L

I

S

A

A

A

M

V

G

G

Q

Q

A

I

F

F

F
|
F

S
x
T

L

L

S
|
S

L

L

G

G

I
x
F

G

G

A

A

M

I

V

I

I

T

Y

Y

G

A

S

S

Y

Y

L

V

P

R

D

K

K

D

T

Q

D

D

I

I

P

V

K

L

A

S

S

G

L

L

Q

T

I

A

C

A

F

T

L

L

T

N

F

E

M

K

I

A

S

E

-

V

L

I

M

L

A

G

G

G

L

S

I

I

I

S

F

I

P

P

S

A

L

A

F

V

A

A

F

F

-

F

-

G

-

V

-

A

-

N

-

A

-

V

G

A

M

I

E

A

P

K

G

A

A

G

G
x
A

A
x
F

G

N

L

L

T

G

F

F

I

I

T

T

L

L

P

P

V

A

V

I

F

F

A

S

K

Q

M

T

P

A

G

G

G

G

V

T

L

F

W

L

A

G

A

F

S

L

F

W

F

F

V

F

L

L

F

L

F

F

F

F

A

A

A

G

L

L

T

T

S

S

S

S

V

I

S

A

L
x
I

L

M

E

Q

V

P

V

M

V

I

S

A

Y

F

M

L

I

E

D

D

S

E

L

L

G

K

W

L

D

S

R

R

K

K

K

H

A

A

T

V

I

L

L

W

L

T

G

A

V

A

L

I

I

V

S

F

V

F

V

-

G

-

I

-

P

-

S

-

A

-

L

-

S

-

Q

-

G

-

A

S

V

A

T

H

I

L

N

V

V

M

F

F

G

L

V

N

S

K

F

S

L

L

D
|
D

A

E

A

M

D
|
D

F

F

F

W

A

A

S

G

N

T

L

I

L

G

M

V

P

V

A

F

G

F

G

G

F

L

L

T

T

E

A

L

V

I

F

I

I

F

G

F

W

W

V

I

-

F

A

G

Y

A

S

D

A

K

G

A

F

W

D

E

G

E

I

I

T

N

G

R

G

G

G

G

A

I

V

I

P

-

F

-

R

K

H

V

K

P

K

R

L

I

W

Y

M

Y

F

Y

I

V

L

M

R

R

F

Y

I

I

A

T

P

P

V

A

C

F

I

L

L

A

V

V

I

L

F

L

I

V

binding leucine: A18 (= A24), G20 (= G26), G22 (= G28), Y104 (= Y105), F244 (= F226), T245 (≠ S227), S247 (= S229), F250 (≠ I232), I350 (≠ L324)
binding (3S)-N-methyl-3-phenyl-3-[4-(trifluoromethyl)phenoxy]propan-1-amine: L21 (= L27), G22 (= G28), R26 (= R32), Y104 (= Y105), A310 (≠ G284), F311 (≠ A285), D392 (= D376), D395 (= D379)

3mpnA F177r1 mutant of leut (see paper)
29% identity, 98% coverage: 7:441/446 of query aligns to 1:459/505 of 3mpnA

query
sites
3mpnA

R

R

G

E

E

H

W

W

S

A

S

T

K

R

I

L

G

G

F

L

I

I

L

L

A

A

A

M

A

A

G

G

S
x
N

A
|
A

I

V

G
|
G

L
|
L

G
|
G

S

N

I

F

W

L

R

R

F

F

P

P

Y

V

I

Q

T

A

G

A

Q

E

S

N

G

G

G

G

A

G

V

A

F

F

V

M

L

I

V

P

Y

Y

L

I

F

I

L

A

V

F

F

L

T

L

I

V

G

G

I

I

S

P

L

L

M

M

M

W

A

I

E

E

I

W

V

A

I

M

G

G

K

R

K

Y

G

G

R

G

L

A

N

Q

A

G

V

H

G

G

S

T

-

T

-

P

-

A

-

I

-

F

-

Y

-

L

-

L

F

W

R

R

R

N

L

R

G

F

G

A

K

K

H

I

W

L

G

G

L

V

V

F

G

G

-

L

W

W

I

I

G

P

V

L

I

V

A

V

A

A

F

I

V

-

I

-

L

-

S

-

Y

Y

Y
|
Y

G

V

V

Y

I

I

G

E

G

S

W

W

T

T

M

L

A

G

Y

F

V

A

L

I

K

K

S

F

F

L

T

V

G

G

L

L

I

V

S

P

T

E

S

P

G

P

S

P

G

T

E

D

V

P

A

D

K

S

I

I

F

L

E

R

A

P

F

F

-

K

-

E

-

F

-

L

-

Y

-

S

-

Y

-

I

-

G

I

V

S

P

D

K

G

G

K

D

Q

E

M

P

L

I

F

L

Y

K

Q

P

G

S

L

L

F

F

-

A

-

Y

-

I

-

V

-

F

-

L

-

I

-

T

M

M

A
x
C

A

I

T

N

V

V

I

S

V

I

V

L

F

I

R

R

G

G

V

I

S

S

G

K

G

G

I

I

E

E

R

R

L

F

C

A

T

K

F

I

C

A

M

M

P

P

A

T

L

L

F

F

I

I

L

L

M

A

I

V

L

F

L

L

I

V

G

I

R

R

A

V

-

F

-

L

L

L

T

E

L

T

P

P

G

N

-

G

-

T

A

A

M

A

E

D

G

G

V

L

V

N

F

F

F

L

L

W

K

T

P

P

D

D

F

F

S

E

K

K

L

L

S

K

G

D

E

P

-

G

V

V

V

W

L

I

S

A

A

A

M

V

G

G

Q

Q

A

I

F

F

F
|
F

S
x
T

L

L

S
|
S

L

L

G

G

I
x
F

G

G

A

A

M

I

V

I

I

T

Y

Y

G

A

S

S

Y

Y

L

V

P

R

D

K

K

D

T

Q

D

D

I

I

P

V

K

L

A

S

S

G

L

L

Q

T

I

A

C

A

F

T

L

L

T

N

F

E

M

K

I

A

S

E

-

V

L

I

M

L

A

G

G

G

L

S

I

I

I

S

F

I

P

P

S

A

L

A

F

V

A

A

F

F

-

F

-

G

-

V

-

A

-

N

-

A

-

V

G

A

M

I

E

A

P

K

G

A

A

G

G

A

A

F

G

N

L

L

T

G

F

F

I

I

T

T

L

L

P

P

V

A

V

I

F

F

A

S

K

Q

M

T

P

A

G

G

G

G

V

T

L

F

W

L

A

G

A

F

S

L

F

W

F

F

V

F

L

L

F

L

F

F

F

F

A

A

A

G

L

L

T

T

S
|
S

S

S

V

I

S

A

L

I

L

M

E

Q

V

P

V

M

V

I

S

A

Y

F

M

L

I

E

D

D

S

E

L

L

G

K

W

L

D

S

R

R

K

K

K

H

A

A

T

V

I

L

L

W

L

T

G
x
A

V

A

L

I

I

V

S

F

V

F

V

-

G

-

I

-

P

-

S

-

A

-

L

-

S

-

Q

-

G

-

A

S

V

A

T

H

I

L

N

V

V

M

F

F

G

L

V

N

S

K

F

S

L

L

D

D

A

E

A

M

D

D

F

F

F

W

A

A

S

G

N

T

L

I

L

G

M

V

P

V

A

F

G

F

G

G

F

L

L

T

T

E

A

L

V

I

F

I

I

F

G

F

W

W

V

I

-

F

A

G

Y

A

S

D

A

K

G

A

F

W

D

E

G

E

I

I

T

N

G

R

G

G

G

G

A

I

V

I

P

-

F

-

R

K

H

V

K

P

K

R

L

I

W

Y

M

Y

F

Y

I

V

L

M

R

R

F

Y

I

I

A

T

P

P

V

A

C

F

I

L

L

A

V

V

I

L

F

L

I

V

binding leucine: N17 (≠ S23), A18 (= A24), G20 (= G26), L21 (= L27), G22 (= G28), Y104 (= Y105), F247 (= F226), T248 (≠ S227), S250 (= S229), F253 (≠ I232), S349 (= S320)
binding S-[(1-oxyl-2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrol-3-yl)methyl] methanesulfonothioate: C171 (≠ A155), A377 (≠ G348)

2qeiA Crystal structure analysis of leut complexed with l-alanine, sodium, and clomipramine (see paper)
29% identity, 98% coverage: 6:441/446 of query aligns to 1:460/511 of 2qeiA

query
sites
2qeiA

S

K

R

R

G

E

E

H

W

W

S

A

S

T

K

R

I

L

G

G

F

L

I

I

L

L

A

A

A

M

A

A

G
|
G

S

N

A
|
A

I
x
V

G
|
G

L

L

G
|
G

S
x
N

I

F

W

L

R
|
R

F

F

P

P

Y
x
V

I
x
Q

T

A

G

A

Q

E

S

N

G

G

G

G

A

G

V

A

F

F

V

M

L

I

V

P

Y

Y

L

I

F

I

L

A

V

F

F

L

T

L

I

V

G

G

I

I

S

P

L

L

M

M

M

W

A

I

E

E

I

W

V

A

I

M

G

G

K

R

K

Y

G

G

R

G

L

A

N

Q

A

G

V

H

G

G