SitesBLAST
Comparing WP_085545458.1 NCBI__GCF_900177735.1:WP_085545458.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
O59010 Glutamate transporter homolog; Glt(Ph); Sodium-aspartate symporter Glt(Ph); Sodium-dependent aspartate transporter from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see 3 papers)
48% identity, 98% coverage: 9:398/400 of query aligns to 11:418/425 of O59010
- S65 (= S57) mutation to V: Strongly decreased chloride conductance.
- R276 (= R261) mutation to S: Increased rate of aspartate transport; when associated with R-395.
- RSS 276:278 (= RSS 261:263) binding L-aspartate
- M311 (= M297) mutation to A: Decreased dependence of aspartate binding on Na(+) concentration.
- T314 (= T300) binding L-aspartate
- V355 (= V341) binding L-aspartate
- D394 (= D374) binding L-aspartate
- M395 (= M375) mutation to R: Increased rate of aspartate transport; when associated with S-276.
- R397 (= R377) mutation to A: Strongly decreased affinity for aspartate.
- N401 (= N381) binding L-aspartate
- D405 (= D385) mutation to N: Strongly decreased affinity for aspartate.
2nwwA Crystal structure of gltph in complex with tboa (see paper)
48% identity, 97% coverage: 9:396/400 of query aligns to 2:407/407 of 2nwwA
6bavA Crystal structure of gltph r397c in complex with s-benzyl-l-cysteine (see paper)
48% identity, 97% coverage: 9:397/400 of query aligns to 3:409/409 of 6bavA
6bauA Crystal structure of gltph r397c in complex with l-cysteine (see paper)
48% identity, 97% coverage: 9:396/400 of query aligns to 3:408/408 of 6bauA
- binding cysteine: S270 (= S263), M303 (= M297), T306 (= T300), A345 (= A339), G346 (= G340), V347 (= V341), G351 (= G345), D386 (= D374), C389 (≠ R377), T390 (= T378), N393 (= N381)
6x15A Inward-facing state of the glutamate transporter homologue gltph in complex with l-aspartate and sodium ions (see paper)
48% identity, 97% coverage: 9:397/400 of query aligns to 11:417/419 of 6x15A
- binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: F46 (≠ V38), F46 (≠ V38), P75 (= P68), L91 (≠ M84), F95 (≠ V88), L130 (= L122), I133 (≠ V125), I159 (≠ L151), Y167 (≠ M158), K196 (= K180), G200 (≠ L184), I207 (≠ Y191), F210 (= F194), L250 (= L234), I262 (≠ F247), M269 (≠ S254), T334 (≠ S320), V335 (≠ I321), G336 (= G322), T340 (≠ G326), L343 (≠ V329), M399 (≠ S379)
- binding aspartic acid: S277 (= S262), S278 (= S263), T314 (= T300), G354 (= G340), A358 (= A344), G359 (= G345), D394 (= D374), R397 (= R377), T398 (= T378)
- binding sodium ion: Y89 (= Y82), T92 (= T85), S93 (≠ T86), G306 (= G292), T308 (= T294), N310 (= N296), N310 (= N296), M311 (= M297), D312 (= D298), S349 (= S335), I350 (≠ V336), T352 (= T338), N401 (= N381), V402 (= V382), D405 (= D385)
Sites not aligning to the query:
6x14A Inward-facing state of the glutamate transporter homologue gltph in complex with tfb-tboa (see paper)
47% identity, 97% coverage: 9:396/400 of query aligns to 8:413/413 of 6x14A
- binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: G66 (= G61), V83 (≠ I79), I157 (≠ F152), Y164 (≠ M158), K193 (= K180), T305 (= T294), I306 (= I295), I347 (≠ V336)
- binding (2~{S},3~{S})-2-azanyl-3-[[3-[[4-(trifluoromethyl)phenyl]carbonylamino]phenyl]methoxy]butanedioic acid: I13 (= I14), M199 (= M186), S275 (= S263), T311 (= T300), G356 (= G345), L384 (≠ A367), D391 (= D374), R394 (= R377)
Sites not aligning to the query:
6zl4A The structure of glutamate transporter homologue glttk in complex with the photo switchable compound (cis) (see paper)
45% identity, 98% coverage: 9:398/400 of query aligns to 6:415/424 of 6zl4A
- binding decyl-beta-d-maltopyranoside: L191 (≠ K180), G195 (≠ L184), R282 (≠ T272)
- binding (2~{S},3~{S})-2-azanyl-3-[[4-[2-(4-methoxyphenyl)hydrazinyl]phenyl]methoxy]butanedioic acid: R271 (= R261), S272 (= S262), S273 (= S263), M307 (= M297), T310 (= T300), G353 (= G343), A354 (= A344), R394 (= R377), T395 (= T378)
Sites not aligning to the query:
6xwnB Structure of glutamate transporter homologue glttk in the presence of tboa inhibitor (see paper)
45% identity, 98% coverage: 9:398/400 of query aligns to 9:418/426 of 6xwnB
6zgbA Glutamate transporter homologue glttk in complex with a photo cage compound (see paper)
45% identity, 98% coverage: 9:398/400 of query aligns to 7:416/425 of 6zgbA
5e9sA Crystal structure of substrate-bound glutamate transporter homologue glttk (see paper)
45% identity, 98% coverage: 9:398/400 of query aligns to 9:418/427 of 5e9sA
- binding aspartic acid: R274 (= R261), S275 (= S262), S276 (= S263), T313 (= T300), G353 (= G340), V354 (= V341), A357 (= A344), G358 (= G345), D394 (= D374), R397 (= R377), T398 (= T378)
- binding decyl-beta-d-maltopyranoside: L194 (≠ K180), G198 (≠ L184), Y202 (≠ L188)
- binding sodium ion: Y87 (= Y82), T90 (= T85), S91 (≠ T86), S276 (= S263), G305 (= G292), A306 (= A293), T307 (= T294), N309 (= N296), N309 (= N296), M310 (= M297), D311 (= D298), S348 (= S335), I349 (≠ V336), G350 (= G337), T351 (= T338), N401 (= N381), V402 (= V382), D405 (= D385)
6r7rA Crystal structure of the glutamate transporter homologue glttk in complex with d-aspartate (see paper)
45% identity, 98% coverage: 9:398/400 of query aligns to 2:407/416 of 6r7rA