SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_085635109.1 NCBI__GCF_002115805.1:WP_085635109.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P96060 2-aminoethylphosphonate--pyruvate transaminase; 2-aminoethylphosphonate aminotransferase; AEP transaminase; AEPT; EC 2.6.1.37 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
47% identity, 90% coverage: 16:370/393 of query aligns to 8:363/367 of P96060

query
sites
P96060

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D168 (= D176) mutation to A: Loss of enzymatic activity.
K194 (= K202) mutation K->L,R: Loss of enzymatic activity.
R340 (= R347) mutation R->A,K: Decreased affinity for all of the substrates.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

1m32A Crystal structure of 2-aminoethylphosphonate transaminase (see paper)
47% identity, 90% coverage: 16:370/393 of query aligns to 3:358/361 of 1m32A

query
sites
1m32A

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binding pyridoxal-5'-phosphate: S60 (= S73), G61 (= G74), S62 (= S75), Y87 (= Y100), T138 (= T151), D163 (= D176), M165 (= M178)
binding phosphonoacetaldehyde: Y87 (= Y100), Y324 (= Y336), R335 (= R347)

1m32B Crystal structure of 2-aminoethylphosphonate transaminase (see paper)
47% identity, 90% coverage: 16:370/393 of query aligns to 4:359/362 of 1m32B

query
sites
1m32B

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binding pyridoxal-5'-phosphate: S61 (= S73), G62 (= G74), S63 (= S75), Y88 (= Y100), T139 (= T151), D164 (= D176), M166 (= M178), F238 (= F249), T239 (= T250)
binding phosphate ion: Y88 (= Y100), Y325 (= Y336)

6pd2C Pntc-aept: fusion protein of phosphonate-specific cytidylyltransferase and 2-aminoethylphosphonate (aep) transaminase from treponema denticola in complex with cytidine monophosphate-aep (see paper)
32% identity, 92% coverage: 16:375/393 of query aligns to 252:614/616 of 6pd2C

query
sites
6pd2C

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G

G

V

M

P

A

G

G

F

V

G

G

F

F

V

V

I

I

A

C

R

N

K

K

D

A

E

E

I

L

E

E

A

K

S

T

K

K

G

D

N

Y

-

P

S

M

H

R

S

N

L

Y

S

Y

L

L

D

N

V

L

H

Y

A

D

Q

Q

W

Y

A

A

T

Y

M

F

E

A

K

K

T

T

G

H

Q

Q

W

T

R

R

F

F

T

T

P

P

T

V

H

Q

V

T

V

M

A

Y

A

A

F

L

L

R

K

Q

A

A

L

V

D

-

L

L

H

E

E

T

A

K

E

Q

G

E

G

T

V

V

S

Q

A

K

R

R

G

Y

A

E

R

R

Y

Y

T

T

R

A

N

C

R

W

D

N

V

I

V

L

V

V

Q

A

G

A

M

I

R

K

D

K

L

L

G

G

F

L

E

K

T

M

L

L

L

V

A

K

D

E

R

E

W

H

L

Q

S

S

P

H

I

F

I

I

V

T

T

A

F

I

F

L

C

E

P

P

A

E

D

T

P

P

S

K

F

Y

T

S

F

F

Q

E

G

A

F

L

Y

H

D

D

A

F

M

A

K

A

A

E

R

H

G

S

F

F

I

T

I

I

Y

Y

P

P

G

G

K

K

L

L

T

G

V

N

V

I

N

D

S

T

F

F

R

R

I

I

G

A

V

N

I

I

G

G

Q

D

M

I

D

Q

E

P

H

E

V

E

M

M

R

R

V

F

V

T

E

V

A

K

A

L

R

K

D

E

A

Y

L

M

T

N

D

G

M

I

N

G

V

V

binding pyridoxal-5'-phosphate: S313 (= S73), G314 (= G74), T315 (≠ S75), Y340 (= Y100), T390 (= T151), D415 (= D176), V417 (≠ M178), S418 (= S179)

Sites not aligning to the query:

binding 5'-O-[(S)-{[(R)-(2-aminoethyl)(hydroxy)phosphoryl]oxy}(hydroxy)phosphoryl]cytidine: 8, 9, 10, 11, 15, 25, 83, 84, 85, 88, 104, 105, 106, 136, 153, 196, 220, 222
binding magnesium ion: 106, 136, 220, 220, 222, 222

Q73MU2 Bifunctional 2-aminoethylphosphonate cytidylyltransferase/aminotransferase; EC 2.7.7.107; EC 2.6.1.37 from Treponema denticola (strain ATCC 35405 / DSM 14222 / CIP 103919 / JCM 8153 / KCTC 15104) (see paper)
32% identity, 92% coverage: 16:375/393 of query aligns to 252:614/616 of Q73MU2

query
sites
Q73MU2

L

L

T

N

P

P

G

G

P

P

L

A

T

T

A

T

Y

D

S

S

V

V

K

K

Q

Y

A

A

M

Q

L

V

K

S

-

A

D

D

W

I

G

C

S

P

W

R

D

E

D

K

D

A

F

F

R

G

A

D

M

L

T

M

R

Q

A

W

L

L

-

C

-

D

R

E

S

L

R

K

L

L

L

F

A

A

L

L

I

A

G

S

G

E

G

T

A

N

S

P

D

D

-

E

Y

Y

D

E

C

T

V

V

P

M

I

F

Q

G

G

C

S
|
S

G
|
G

S
x
T

Y

G

C

A

V

D

E

E

A

V

M

M

L

V

G

S

S

S

F

C

V

V

P

P

R

D

D

T

G

G

K

R

V

L

L

L

V

V

L

I

A

D

N

N

G

G

A

S

Y

Y

G

G

L

A

R

R

A

M

A

A

Q

K

T

I

L

A

G

D

Y

I

L

Y

E

K

R

I

D

P

Y

M

H

D

L

I

L

F

D

K

K

S

G

S

D

T

Y

Y

L

E

P

P

P

L

R

D

G

L

D

Q

E

K

V

L

A

E

A

A

I

E

L

F

A

A

A

T

D

-

P

K

A

K

I

Y

T

T

H

H

V

L

L

A

A

C

I

V

H

Y

C

H

E

E

T
|
T

S

T

G

G

I

L

Q

L

N

N

P

P

V

L

E

H

E

I

I

I

A

C

E

P

A

M

T

A

K

K

A

K

A

Y

G

G

R

M

K

V

L

T

L

I

I

V

D

D

S

A

M

V

S

S

A

A

F

Y

G

C

A

G

L

M

P

P

L

M

D

D

P

L

V

K

K

S

L

L

N

G

C

I

A

D

A

F

F

M

V

A

S

S

S

T

A

S

N

N

K
|
K

C

N

I

I

E

Q

G

G

V

M

P

A

G

G

F

V

G

G

F

F

V

V

I

I

A

C

R

N

K

K

D

A

E

E

I

L

E

E

A

K

S

T

K

K

G

D

N

Y

-

P

S

M

H

R

S

N

L

Y

S

Y

L

L

D

N

V

L

H

Y

A

D

Q

Q

W

Y

A

A

T

Y

M

F

E

A

K

K

T

T

G

H

Q

Q

W

T

R

R

F

F

T
|
T

P

P

T

V

H

Q

V

T

V

M

A

Y

A

A

F

L

L

R

K

Q

A

A

L

V

D

-

L

L

H

E

E

T

A

K

E

Q

G

E

G

T

V

V

S

Q

A

K

R

R

G

Y

A

E

R

R

Y

Y

T

T

R

A

N

C

R

W

D

N

V

I

V

L

V

V

Q

A

G

A

M

I

R

K

D

K

L

L

G

G

F

L

E

K

T

M

L

L

L

V

A

K

D

E

R

E

W

H

L

Q

S

S

P

H

I

F

I

I

V

T

T

A

F

I

F

L

C

E

P

P

A

E

D

T

P

P

S

K

F

Y

T

S

F

F

Q

E

G

A

F

L

Y

H

D

D

A

F

M

A

K

A

A

E

R

H

G

S

F

F

I

T

I

I

Y

Y

P

P

G

G

K

K

L

L

T

G

V

N

V

I

N

D

S

T

F

F

R

R

I

I

G

A

V

N

I

I

G

G

Q

D

M

I

D

Q

E

P

H

E

V

E

M

M

R

R

V

F

V

T

E

V

A

K

A

L

R

K

D

E

A

Y

L

M

T

N

D

G

M

I

N

G

V

V

S313 (= S73) binding pyridoxal 5'-phosphate
G314 (= G74) binding pyridoxal 5'-phosphate
T315 (≠ S75) binding pyridoxal 5'-phosphate
T390 (= T151) binding pyridoxal 5'-phosphate
K441 (= K202) binding pyridoxal 5'-phosphate
T490 (= T250) binding pyridoxal 5'-phosphate

Sites not aligning to the query:

8 binding CMP-(2-aminoethyl)phosphonate
10 binding CMP-(2-aminoethyl)phosphonate
11 binding CMP-(2-aminoethyl)phosphonate
15 R→A: Strong decrease in cytidylyltransferase activity.
25 binding CMP-(2-aminoethyl)phosphonate; K→A: Almost loss of cytidylyltransferase activity.
83 binding CMP-(2-aminoethyl)phosphonate
88 binding CMP-(2-aminoethyl)phosphonate
104 binding CMP-(2-aminoethyl)phosphonate
105 binding CMP-(2-aminoethyl)phosphonate
106 binding Mg(2+)
136 binding CMP-(2-aminoethyl)phosphonate; binding Mg(2+)
153 binding CMP-(2-aminoethyl)phosphonate; K→A: Strong decrease in cytidylyltransferase activity.
196 binding CMP-(2-aminoethyl)phosphonate
220 binding Mg(2+); binding Mg(2+)
222 binding Mg(2+); binding Mg(2+)

2dr1A Crystal structure of the ph1308 protein from pyrococcus horikoshii ot3
26% identity, 91% coverage: 16:374/393 of query aligns to 19:376/381 of 2dr1A

query
sites
2dr1A

L

L

L

F

T

T

P

A

G

G

P

P

L

V

T

A

T

C

A

F

Y

P

S

E

V

V

K

L

Q

E

A

I

M

M

-

K

L

V

K

Q

D

M

W

F

G

S

S

H

W

R

D

S

D

K

D

E

F

Y

R

R

A

K

M

V

T

H

R

M

A

D

L

T

R

V

S

E

R

R

L

L

L

R

A

E

L

F

I

L

G

E

G

V

G

E

A

K

S

G

D

E

Y

V

D

L

C

L

V

V

P

P

I

S

Q
x
S

G
|
G

S
x
T

G

G

S

-

Y

-

C

I

V

M

E

E

A

A

M

S

L

I

G

R

S

N

F

G

V

V

P

S

R

K

D

G

G

G

K

K

V

V

L

L

V

V

L

T

A

I

N

I

G

G

A

A

Y
x
F

G

G

L

K

R

R

A

Y

A

K

Q

E

T

V

L

V

G

E

Y

S

L

N

E

G

R

R

D

K

Y

A

H

V

L

V

L

L

D

E

K

-

G

-

D

-

Y

Y

L

E

P

P

P

G

R

K

G

A

-

V

-

K

-

P

D

E

E

D

V

L

A

D

I

A

L

L

A

R

A

K

D

N

P

P

A

D

I

V

T

E

H

A

V

V

L

T

A

I

I

T

H

Y

C

N

E

E

T
|
T

S

S

S

T

G

G

I

V

Q

L

N

N

P

P

V

L

E

P

E

E

I

L

A

A

E

K

A

V

T

A

K

K

A

E

A

H

G

D

R

K

K

L

L

V

L

F

I

V

D
|
D

S

A

M
x
V

S

S

A

A

F

M

G

G

A

G

L

A

P

D

L

I

D

K

P

F

V

D

K

K

L

W

N

G

C

L

A

D

A

V

F

V

V

F

S

S

S
|
S

A

S

N

Q

K
|
K

C

A

I

F

E

-

G

G

V

V

P

P

-

P

-

G

-

L

G

A

F

I

G

G

F

A

V

F

I

S

A

E

R

R

K

F

D

L

E

E

I

I

E

-

A

A

S

E

K

K

G

M

N

P

S

E

H

R

S

G

L

W

S

Y

L

F

D

D

V

I

H

P

A

L

Q

Y

W

V

A

K

T

Y

M

L

E

K

K

E

T

K

G

E

Q

S

W

T

R

P

F

S

T

T

P

P

T

M

H

P

V

Q

V

V

A

F

A

G

F

I

L

N

K

V

A

A

L

L

D

R

L

I

H

I

E

E

A

K

E

M

G

G

V

K

S

E

A

K

R

W

G

L

A

E

R

M

Y

Y

T

E

R

K

N

R

R

A

D

K

V

M

V

V

V

R

Q

E

G

G

M

V

R

R

D

E

L

I

G

G

F

L

E

D

T

I

L

L

L

A

A

E

D

P

R

G

W

H

L

E

S

S

P

P

I

T

I

I

V

T

T

A

F

V

F

L

C

T

P

P

A

-

D

-

P

P

S

G

F

I

T

K

F

G

Q

D

G

E

F

V

Y

Y

D

E

A

A

M

M

K

R

A

K

R

R

G

G

F

F

I

E

I

L

Y

A

P

K

G

G

K

Y

L

G

T

S

V

V

V

K

-

E

N

K

S

T

F

F

R

R

I

I

G

G

V

H

I

M

G

G

Q

Y

M

M

D

K

E

F

H

E

V

D

M

I

R

Q

R

E

V

M

V

L

E

D

A

N

A

L

R

R

D

E

A

V

L

I

T

N

D

E

M

L

N

K

binding pyridoxal-5'-phosphate: S75 (≠ Q71), G76 (= G72), T77 (≠ S73), F102 (≠ Y100), T153 (= T151), D178 (= D176), V180 (≠ M178), S201 (= S199), K204 (= K202)

2yrrA Hypothetical alanine aminotransferase (tth0173) from thermus thermophilus hb8
31% identity, 71% coverage: 16:295/393 of query aligns to 3:276/352 of 2yrrA

query
sites
2yrrA

L

L

T

T

P

P

G

G

P

P

L

T

T

P

T

I

A

P

Y

E

S

R

V

V

K

Q

Q

K

A

A

M

L

L

L

K

R

D

P

W

M

-

R

G

G

S

H

W

L

D

D

D

P

D

E

F

V

R

L

A

R

M

V

T

N

R

R

A

A

L

I

R

Q

S

E

R

R

L

L

L

A

A

A

L

L

I

F

G

D

G

P

G

G

A

E

S

G

D

A

Y

L

D

-

C

V

V

A

P

A

I

L

Q

A

G

G

S
|
S

G
|
G

S
|
S

Y

L

C

G

V

M

E

E

A

A

M

G

L

L

G

A

S

N

F

L

V

-

P

-

R

D

D

R

G

G

K

P

V

V

L

L

V

V

L

L

A

V

N

N

G

G

A

A

Y
x
F

G

S

L

Q

R

R

A

V

A

A

Q

E

T

M

L

A

G

A

Y

L

L

H

E

G

R

L

D

D

Y

P

H

E

L

V

L

L

D

D

K

F

G

P

D

P

Y

G

L

E

P

P

P

V

R

D

G

P

D

E

E

A

V

V

A

A

A

R

I

A

L

L

A

K

A

R

D

R

P

-

A

R

I

Y

T

R

H

M

V

V

L

A

A

L

I

V

H

H

C

G

E

E

T
|
T

S

S

S

T

G

G

I

V

Q

L

N

N

P

P

V

A

E

E

E

A

I

I

A

G

E

A

A

L

T

A

K

K

A

E

A

A

G

G

R

A

K

L

L

F

I

L

D
|
D

S

A

M
x
V

S

T

A

T

F

L

G

G

A

M

L

L

P

P

L

F

D

S

P

M

V

R

K

A

L

M

N

G

C

V

A

D

A

Y

F

A

V

F

S

T

S

G

A

S

N

Q

K
|
K

C

C

I

L

E

S

G

A

V

P

P

P

G

G

F

L

G

A

F

P

V

I

I

A

A

A

R

S

K

L

D

E

E

A

I

R

E

K

A

A

S

F

K

T

G

G

N

K

S

-

H

R

S

G

L

W

S

Y

L

L

D

D

V

L

H

-

A

A

Q

R

W

V

A

A

T

E

M

H

E

W

K

E

T

R

G

G

Q

G

W

Y

R

H

F

H

T

T

P

T

P

P

T

V

H

L

V

L

V

H

A

Y

A

A

F

L

L

L

K

E

A

A

L

L

D

D

L

L

H

-

E

V

A

L

E

E

G

E

G

G

V

V

S

A

A

A

R

R

G

E

A

R

R

R

Y

A

T

R

R

E

N

V

R

Y

D

A

V

W

V

V

V

L

Q

E

G

E

M

L

R

K

D

A

L

R

G

G

F

F

binding pyridoxal-5'-phosphate: S60 (= S73), G61 (= G74), S62 (= S75), F85 (≠ Y100), T135 (= T151), D160 (= D176), V162 (≠ M178), K186 (= K202)

2yriA Crystal structure of alanine-pyruvate aminotransferase with 2- methylserine
31% identity, 71% coverage: 16:295/393 of query aligns to 3:276/352 of 2yriA

query
sites
2yriA

L

L

T

T

P

P

G
|
G

P

P

L

T

T

P

T

I

A

P

Y

E

S

R

V

V

K

Q

Q

K

A

A

M

L

L

L

K

R

D

P

W

M

-

R

G

G

S

H

W

L

D

D

D

P

D

E

F

V

R

L

A

R

M

V

T

N

R

R

A

A

L

I

R

Q

S

E

R

R

L

L

L

A

A

A

L

L

I

F

G

D

G

P

G

G

A

E

S

G

D

A

Y

L

D

-

C

V

V

A

P

A

I

L

Q

A

G

G

S
|
S

G
|
G

S
|
S

Y

L

C

G

V

M

E

E

A

A

M

G

L

L

G

A

S

N

F

L

V

-

P

-

R

D

D

R

G

G

K

P

V

V

L

L

V

V

L

L

A

V

N

N

G

G

A

A

Y
x
F

G

S

L

Q

R

R

A

V

A

A

Q

E

T

M

L

A

G

A

Y

L

L

H

E

G

R

L

D

D

Y

P

H

E

L

V

L

L

D

D

K

F

G

P

D

P

Y

G

L

E

P

P

P

V

R

D

G

P

D

E

E

A

V

V

A

A

A

R

I

A

L

L

A

K

A

R

D

R

P

-

A

R

I

Y

T

R

H

M

V

V

L

A

A

L

I

V

H

H

C

G

E

E

T
|
T

S

S

S

T

G

G

I

V

Q

L

N

N

P

P

V

A

E

E

E

A

I

I

A

G

E

A

A

L

T

A

K

K

A

E

A

A

G

G

R

A

K

L

L

F

I

L

D
|
D

S

A

M
x
V

S

T

A

T

F

L

G

G

A

M

L

L

P

P

L

F

D

S

P

M

V

R

K

A

L

M

N

G

C

V

A

D

A

Y

F

A

V

F

S

T

S

G

A

S

N

Q

K
|
K

C

C

I

L

E

S

G

A

V

P

P

P

G

G

F

L

G

A

F

P

V

I

I

A

A

A

R

S

K

L

D

E

E

A

I

R

E

K

A

A

S

F

K

T

G

G

N

K

S

-

H

R

S

G

L

W

S

Y

L

L

D

D

V

L

H

-

A

A

Q

R

W

V

A

A

T

E

M

H

E

W

K

E

T

R

G

G

Q

G

W
x
Y

R

H

F

H

T
|
T

P

T

P

P

T

V

H

L

V

L

V

H

A

Y

A

A

F

L

L

L

K

E

A

A

L

L

D

D

L

L

H

-

E

V

A

L

E

E

G

E

G

G

V

V

S

A

A

A

R

R

G

E

A

R

R

R

Y

A

T

R

R

E

N

V

R

Y

D

A

V

W

V

V

V

L

Q

E

G

E

M

L

R

K

D

A

L

R

G

G

F

F

binding (s,e)-3-hydroxy-2-((3-hydroxy-2-methyl-5-(phosphonooxymethyl)pyridin-4-yl)methyleneamino)-2-methylpropanoic acid: G7 (= G20), S60 (= S73), G61 (= G74), S62 (= S75), F85 (≠ Y100), T135 (= T151), D160 (= D176), V162 (≠ M178), K186 (= K202)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: Y229 (≠ W247), T232 (= T250)

Sites not aligning to the query:

binding (s,e)-3-hydroxy-2-((3-hydroxy-2-methyl-5-(phosphonooxymethyl)pyridin-4-yl)methyleneamino)-2-methylpropanoic acid: 326

2z9xA Crystal structure of pyridoxamine-pyruvate aminotransferase complexed with pyridoxyl-l-alanine (see paper)
28% identity, 73% coverage: 68:352/393 of query aligns to 62:349/392 of 2z9xA

query
sites
2z9xA

V

V

P

I

I

L

Q

H

G

G

S
x
E

G

P

S

V

Y

L

C

G

V

L

E

E

A

A

M

A

L

A

G

A

S

S

F

L

V

I

P

S

R

P

D

D

G

D

K

V

V

V

L

L

V

N

L

L

A

A

N

S

G

G

A

V

Y
|
Y

G

G

L

-

R

-

A

-

Q

K

T

G

L

F

G

G

Y

Y

L

W

E

A

R

K

D

R

Y

Y

-

S

-

P

H

H

L

L

D

E

-

I

-

E

-

V

-

P

K

Y

G

N

D

E

Y

A

L

I

P

D

P

P

R

Q

G

A

D

-

E

-

V

V

A

A

A

D

I

M

L

L

A

K

A

A

D

H

P

P

A

E

I

I

T

T

H

V

V

V

L

S

A

V

I

C

H

H

C

H

E

D

T
|
T

S

P

S

S

G

G

I

T

Q

I

N

N

P

P

V

I

E

D

E

A

I

I

A

G

E

A

A

L

T

V

K

S

A

A

A

H

G

G

R

A

K

Y

L

L

L

I

I

V

D
|
D

S

A

M
x
V

S

S

A

S

F

F

G

G

A

G

L

M

P

K

L

T

D

H

P

P

V

E

K

D

L

C

N

K

C

A

A

D

A

I

F

Y

V

V

S

T

S

G

A

P

N

N

K
|
K

C

C

I

L

E

G

G

A

V

P

P

P

G

G

F

L

G

T

F

M

V

M

I

G

A

V

R

S

K

E

D

R

E

A

I

W

E

A

A

K

S

M

K

K

G

A

N

N

-

P

-

L

-

A

-

P

-

R

S

A

H

S

S

M

L

L

S

S

L

I

-

V

D

D

V

W

H

E

A

N

Q

A

W

W

A

S

T

-

M

-

E

-

K

R

T

D

G

K

Q

P

W

F

R

P

F

F

T

T

P

P

P

S

T

V

H

S

V

E

V

I

A

N

A

G

F

L

L

D

K

V

A

A

L

L

D

D

L

L

H

Y

E

L

A

N

E

E

G

G

G

P

V

-

S

E

A

A

R

V

G

W

A

A

R

R

Y

H

T

A

R

L

N

T

R

A

D

K

V

A

V

M

V

R

Q

A

G

G

M

V

R

T

D

A

L

M

G

G

F

L

E

S

T

V

L

W

L

A

A

A

-

S

D

D

R

S

W

I

L

A

S

S

P

P

I

T

V

T

T

A

F

V

F

R

C

T

P

P

A

D

D

-

P

-

S

G

F

V

T

D

F

E

Q

K

G

A

F

L

Y

R

D

Q

A

A

M

A

K

R

A

A

R

R

G

Y

F

G

I

V

Y

F

P

S

-

S

-

G

-

R

-

G

-

E

-

T

-

L

G

G

K

K

L

L

T

T

V

-

N

-

S

-

F

-

R
|
R

I

I

G

G

V

H

I

M

G

G

binding alanine: Y94 (= Y100), R344 (= R347)
binding 3-hydroxy-5-(hydroxymethyl)-2-methylisonicotinaldehyde: E67 (≠ S73), Y94 (= Y100), T145 (= T151), D170 (= D176), V172 (≠ M178), K196 (= K202)

2z9wA Crystal structure of pyridoxamine-pyruvate aminotransferase complexed with pyridoxal (see paper)
28% identity, 73% coverage: 68:352/393 of query aligns to 62:349/392 of 2z9wA

query
sites
2z9wA

V

V

P

I

I

L

Q

H

G

G

S
x
E

G
x
P

S

V

Y

L

C

G

V

L

E

E

A

A

M

A

L

A

G

A

S

S

F

L

V

I

P

S

R

P

D

D

G

D

K

V

V

V

L

L

V

N

L

L

A

A

N

S

G

G

A

V

Y
|
Y

G

G

L

-

R

-

A

-

Q

K

T

G

L

F

G

G

Y

Y

L

W

E

A

R

K

D

R

Y

Y

-

S

-

P

H

H

L

L

D

E

-

I

-

E

-

V

-

P

K

Y

G

N

D

E

Y

A

L

I

P

D

P

P

R

Q

G

A

D

-

E

-

V

V

A

A

A

D

I

M

L

L

A

K

A

A

D

H

P

P

A

E

I

I

T

T

H

V

V

V

L

S

A

V

I

C

H

H

C

H

E

D

T
|
T

S

P

S

S

G

G

I

T

Q

I

N

N

P

P

V

I

E

D

E

A

I

I

A

G

E

A

A

L

T

V

K

S

A

A

A

H

G

G

R

A

K

Y

L

L

L

I

I

V

D
|
D

S

A

M
x
V

S
|
S

A

S

F

F

G

G

A

G

L

M

P

K

L

T

D

H

P

P

V

E

K

D

L

C

N

K

C

A

A

D

A

I

F

Y

V

V

S

T

S

G

A

P

N

N

K
|
K

C

C

I

L

E

G

G

A

V

P

P

P

G

G

F

L

G

T

F

M

V

M

I

G

A

V

R

S

K

E

D

R

E

A

I

W

E

A

A

K

S

M

K

K

G

A

N

N

-

P

-

L

-

A

-

P

-

R

S

A

H

S

S

M

L

L

S

S

L

I

-

V

D

D

V

W

H

E

A

N

Q

A

W

W

A

S

T

-

M

-

E

-

K

R

T

D

G

K

Q

P

W

F

R

P

F

F

T

T

P

P

P

S

T

V

H

S

V

E

V

I

A

N

A

G

F

L

L

D

K

V

A

A

L

L

D

D

L

L

H

Y

E

L

A

N

E

E

G

G

G

P

V

-

S

E

A

A

R

V

G

W

A

A

R

R

Y

H

T

A

R

L

N

T

R

A

D

K

V

A

V

M

V

R

Q

A

G

G

M

V

R

T

D

A

L

M

G

G

F

L

E

S

T

V

L

W

L

A

A

A

-

S

D

D

R

S

W

I

L

A

S

S

P

P

I

T

V

T

T

A

F

V

F

R

C

T

P

P

A

D

D

-

P

-

S

G

F

V

T

D

F

E

Q

K

G

A

F

L

Y

R

D

Q

A

A

M

A

K

R

A

A

R

R

G

Y

F

G

I

V

Y

F

P

S

-

S

-

G

-

R

-

G

-

E

-

T

-

L

G

G

K

K

L

L

T

T

V

-

N

-

S

-

F

-

R

R

I

I

G

G

V

H

I

M

G

G

binding 3-hydroxy-5-(hydroxymethyl)-2-methylisonicotinaldehyde: E67 (≠ S73), P68 (≠ G74), Y94 (= Y100), T145 (= T151), D170 (= D176), V172 (≠ M178), S173 (= S179), K196 (= K202)

2z9vA Crystal structure of pyridoxamine-pyruvate aminotransferase complexed with pyridoxamine (see paper)
28% identity, 73% coverage: 68:352/393 of query aligns to 62:349/392 of 2z9vA

query
sites
2z9vA

V

V

P

I

I

L

Q

H

G

G

S
x
E

G

P

S
x
V

Y

L

C

G

V

L

E

E

A

A

M

A

L

A

G

A

S

S

F

L

V

I

P

S

R

P

D

D

G

D

K

V

V

V

L

L

V

N

L

L

A

A

N

S

G

G

A

V

Y
|
Y

G

G

L

-

R

-

A

-

Q

K

T

G

L

F

G

G

Y

Y

L

W

E

A

R

K

D

R

Y

Y

-

S

-

P

H

H

L

L

D

E

-

I

-

E

-

V

-

P

K

Y

G

N

D

E

Y

A

L

I

P

D

P

P

R

Q

G

A

D

-

E

-

V

V

A

A

A

D

I

M

L

L

A

K

A

A

D

H

P

P

A

E

I

I

T

T

H

V

V

V

L

S

A

V

I

C

H

H

C

H

E

D

T
|
T

S

P

S

S

G

G

I

T

Q

I

N

N

P

P

V

I

E

D

E

A

I

I

A

G

E

A

A

L

T

V

K

S

A

A

A

H

G

G

R

A

K

Y

L

L

L

I

I

V

D
|
D

S

A

M
x
V

S

S

A

S

F

F

G

G

A

G

L

M

P

K

L

T

D

H

P

P

V

E

K

D

L

C

N

K

C

A

A

D

A

I

F

Y

V

V

S

T

S

G

A

P

N

N

K

K

C

C

I

L

E

G

G

A

V

P

P

P

G

G

F

L

G

T

F

M

V

M

I

G

A

V

R

S

K

E

D

R

E

A

I

W

E

A

A

K

S

M

K

K

G

A

N

N

-

P

-

L

-

A

-

P

-

R

S

A

H

S

S

M

L

L

S

S

L

I

-

V

D

D

V

W

H

E

A

N

Q

A

W

W

A

S

T

-

M

-

E

-

K

R

T

D

G

K

Q

P

W

F

R

P

F

F

T

T

P

P

P

S

T

V

H

S

V

E

V

I

A

N

A

G

F

L

L

D

K

V

A

A

L

L

D

D

L

L

H

Y

E

L

A

N

E

E

G

G

G

P

V

-

S

E

A

A

R

V

G

W

A

A

R

R

Y

H

T

A

R

L

N

T

R

A

D

K

V

A

V

M

V

R

Q

A

G

G

M

V

R

T

D

A

L

M

G

G

F

L

E

S

T

V

L

W

L

A

A

A

-

S

D

D

R

S

W

I

L

A

S

S

P

P

I

T

V

T

T

A

F

V

F

R

C

T

P

P

A

D

D

-

P

-

S

G

F

V

T

D

F

E

Q

K

G

A

F

L

Y

R

D

Q

A

A

M

A

K

R

A

A

R

R

G

Y

F

G

I

V

Y

F

P

S

-

S

-

G

-

R

-

G

-

E

-

T

-

L

G

G

K

K

L

L

T

T

V

-

N

-

S

-

F

-

R

R

I

I

G

G

V

H

I

M

G

G

binding 4-(aminomethyl)-5-(hydroxymethyl)-2-methylpyridin-3-ol: E67 (≠ S73), V69 (≠ S75), Y94 (= Y100), T145 (= T151), D170 (= D176), V172 (≠ M178)

Q988B8 Pyridoxamine--pyruvate transaminase; Pyridoxamine-pyruvate aminotransferase; EC 2.6.1.30 from Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099) (Mesorhizobium loti (strain MAFF 303099)) (see 2 papers)
28% identity, 73% coverage: 68:352/393 of query aligns to 63:350/393 of Q988B8

query
sites
Q988B8

V

V

P

I

I

L

Q

H

G

G

S
x
E

G

P

S

V

Y

L

C

G

V

L

E

E

A

A

M

A

L

A

G

A

S

S

F

L

V

I

P

S

R

P

D

D

G

D

K

V

V

V

L

L

V

N

L

L

A

A

N

S

G

G

A

V

Y
|
Y

G

G

L

-

R

-

A

-

Q

K

T

G

L

F

G

G

Y

Y

L

W

E

A

R

K

D

R

Y

Y

-

S

-

P

H

H

L

L

D

E

-

I

-

E

-

V

-

P

K

Y

G

N

D

E

Y

A

L

I

P

D

P

P

R

Q

G

A

D

-

E

-

V

V

A

A

A

D

I

M

L

L

A

K

A

A

D

H

P

P

A

E

I

I

T

T

H

V

V

V

L

S

A

V

I

C

H

H

C

H

E

D

T
|
T

S

P

S

S

G

G

I

T

Q

I

N

N

P

P

V

I

E

D

E

A

I

I

A

G

E

A

A

L

T

V

K

S

A

A

A

H

G

G

R

A

K

Y

L

L

L

I

I

V

D

D

S

A

M

V

S

S

A

S

F

F

G

G

A

G

L

M

P

K

L

T

D

H

P

P

V

E

K

D

L

C

N

K

C

A

A

D

A

I

F

Y

V

V

S

T

S

G

A

P

N

N

K
|
K

C
|
C

I

L

E

G

G

A

V

P

P

P

G

G

F

L

G

T

F

M

V

M

I

G

A

V

R

S

K

E

D

R

E

A

I

W

E

A

A

K

S

M

K

K

G

A

N

N

-

P

-

L

-

A

-

P

-

R

S

A

H

S

S

M

L

L

S

S

L

I

-

V

D

D

V

W

H

E

A

N

Q

A

W

W

A

S

T

-

M

-

E

-

K

R

T

D

G

K

Q

P

W

F

R

P

F

F

T

T

P

P

P

S

T

V

H

S

V

E

V

I

A

N

A

G

F

L

L

D

K

V

A

A

L

L

D

D

L

L

H

Y

E

L

A

N

E

E

G

G

G

P

V

-

S

E

A

A

R

V

G

W

A

A

R

R

Y

H

T

A

R

L

N

T

R

A

D

K

V

A

V

M

V

R

Q

A

G

G

M

V

R

T

D

A

L

M

G

G

F

L

E

S

T

V

L

W

L

A

A

A

-

S

D

D

R

S

W

I

L

A

S

S

P

P

I

T

V

T

T

A

F

V

F

R

C

T

P

P

A

D

D

-

P

-

S

G

F

V

T

D

F

E

Q

K

G

A

F

L

Y

R

D

Q

A

A

M

A

K

R

A

A

R

R

G

Y

F

G

I

V

Y

F

P

S

-

S

-

G

-
x
R

-

G

-

E

-

T

-

L

G

G

K

K

L

L

T

T

V

-

N

-

S

-

F

-

R
|
R

I

I

G

G

V

H

I

M

G

G

E68 (≠ S73) binding pyridoxal 5'-phosphate; mutation E->A,G: Low but detectable pyridoxamine--pyruvate transaminase activity.
Y95 (= Y100) binding pyridoxal 5'-phosphate
T146 (= T151) binding pyridoxal 5'-phosphate
K197 (= K202) modified: N6-(pyridoxal phosphate)lysine; mutation to L: Loss of function.
C198 (= C203) mutation to A: No effect on enzyme activity.
R336 (vs. gap) mutation to A: Strongly decreased affinity for pyruvate.
R345 (= R347) binding pyridoxal 5'-phosphate

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

3zrrA Crystal structure and substrate specificity of a thermophilic archaeal serine : pyruvate aminotransferase from sulfolobus solfataricus (see paper)
25% identity, 86% coverage: 16:352/393 of query aligns to 1:338/379 of 3zrrA

query
sites
3zrrA

L

L

T

H

P
x
V

G

G

P

P

L

T

T

T

T

I

A

K

Y

E

S

D

V

V

K

L

Q

V

A

A

M

G

L

L

K

E

D

N

-

N

W

V

G

G

S
x
F

W

T

D

S

D

K

D

E

F

F

-

V

R

E

A

A

M

L

T

A

R

Y

A

S

L

L

R

K

S

G

R

-

L

-

L

L

A

R

L

Y

I

V

G

M

G

G

G

A

A

S

S

K

D

N

Y

Y

D

Q

C

P

V

L

P

I

I

I

Q

P

G

G

S
x
G

G
|
G

S
x
T

Y

S

C

A

V

M

E

E

A

S

M

V

L

T

G

S

S

L

F

L

V

K

P

P

R

N

D

D

G

-

K

K

V

I

L

L

V

V

L

V

A

S

N

N

G

G

A

V

Y
x
F

G

G

L

D

R

R

A

W

A

E

Q

Q

T

I

L

F

G

K

Y

R

L

Y

E

P

R

V

D

N

Y

V

H

K

L

V

L

L

-

R

-

P

D

S

K

P

G

G

D

D

Y

Y

L

V

P

K

P

P

R

-

G

G

D

E

E

V

V

E

A

E

I

V

L

R

A

K

A

S

D

E

P

Y

A

K

I

L

T

-

H

-

V

V

L

A

A

L

I

T

H

H

C

V

E

E

T
|
T

S

S

S

T

G

G

I

V

Q

R

N

E

P

P

V

V

E

K

E

D

I

V

A

I

E

N

A

K

T

I

K

R

A

K

A

Y

G

V

R

E

K

L

L

I

L

V

I

V

D
|
D

S

G

M
x
V

S

S

A

S

F

V

G

G

A

A

L

E

P

E

L

V

D

K

P

A

V

E

K

E

L

W

N

N

C

V

A

D

A

V

F

Y

V

L

S

T

S

A

A

S

N

Q

K

K

C

A

I

L

E

G

G

S

V

A

P

A

G

G

F

L

G

G

F

L

V

L

I

L

A

L

-

S

-

P

R

K

K

A

D

L

E

S

I

I

E

L

A

D

S

S

K

Q

G

N

N

S

S

I

H

A

S

G

L

Y

S

Y

L

L

D

D

V

L

H

R

A

N

Q

W

W

L

A

P

T

V

M

M

-

R

-

G

-

A

-

E

E

E

K

G

T

K

G

A

Q

A

W
x
Y

R

F

F

A

T
|
T

P

P

T

V

H

H

V

V

V

I

A

L

A

Q

F

L

L

A

K

E

A

A

L

F

D

R

L

L

H

I

E

E

A

K

E

E

G

-

G

G

V

I

S

E

A

N

R

R

G

I

A

K

R

R

Y

H

T

T

R

M

N

V

R

A

D

S

V

A

V

I

V

R

Q

A

G

G

M

L

R

E

D

A

L

L

G

G

F

L

E

E

T

-

L

I

L

V

A

A

D

R

R

R

-

P

-

E

-

S

W

Y

L

S

S

N

P

T

I

V

I

T

V

G

T

V

F

I

F

L

C

K

P

V

A

A

D

D

P

P

S

Q

F

K

T

V

F

L

Q

A

G

G

F

T

Y

V

D

N

A

-

M

-

K

-

A

-

R

E

G

G

F

V

I

E

I

F

Y

A

P

P

G

G

K

V

L

H

T

P

V

A

V

F

N

K

S

Y

F

F

R
|
R

I

I

G

G

V

H

I

M

G

G

binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: V4 (≠ P19), F24 (≠ S38), G58 (≠ S73), G59 (= G74), T60 (≠ S75), F84 (≠ Y100), T134 (= T151), D159 (= D176), V161 (≠ M178), Y236 (≠ W247), T239 (= T250), R333 (= R347)

3zrqA Crystal structure and substrate specificity of a thermophilic archaeal serine : pyruvate aminotransferase from sulfolobus solfataricus (see paper)
25% identity, 86% coverage: 16:352/393 of query aligns to 4:341/382 of 3zrqA

query
sites
3zrqA

L

L

T

H

P

V

G

G

P

P

L

T

T

T

T

I

A

K

Y

E

S

D

V

V

K

L

Q

V

A

A

M

G

L

L

K

E

D

N

-

N

W

V

G

G

S

F

W

T

D

S

D

K

D

E

F

F

-

V

R

E

A

A

M

L

T

A

R

Y

A

S

L

L

R

K

S

G

R

-

L

-

L

L

A

R

L

Y

I

V

G

M

G

G

G

A

A

S

S

K

D

N

Y

Y

D

Q

C

P

V

L

P

I

I

I

Q

P

G

G

S

G

G
|
G

S
x
T

Y

S

C

A

V

M

E

E

A

S

M

V

L

T

G

S

S

L

F

L

V

K

P

P

R

N

D

D

G

-

K

K

V

I

L

L

V

V

L

V

A

S

N

N

G

G

A

V

Y
x
F

G

G

L

D

R

R

A

W

A

E

Q

Q

T

I

L

F

G

K

Y

R

L

Y

E

P

R

V

D

N

Y

V

H

K

L

V

L

L

-

R

-

P

D

S

K

P

G

G

D

D

Y

Y

L

V

P

K

P

P

R

-

G

G

D

E

E

V

V

E

A

E

I

V

L

R

A

K

A

S

D

E

P

Y

A

K

I

L

T

-

H

-

V

V

L

A

A

L

I

T

H

H

C

V

E

E

T
|
T

S

S

S

T

G

G

I

V

Q

R

N

E

P

P

V

V

E

K

E

D

I

V

A

I

E

N

A

K

T

I

K

R

A

K

A

Y

G

V

R

E

K

L

L

I

L

V

I

V

D
|
D

S

G

M
x
V

S

S

A

S

F

V

G

G

A

A

L

E

P

E

L

V

D

K

P

A

V

E

K

E

L

W

N

N

C

V

A

D

A

V

F

Y

V

L

S

T

S

A

A

S

N

Q

K
|
K

C

A

I

L

E

G

G

S

V

A

P

A

G

G

F

L

G

G

F

L

V

L

I

L

A

L

-

S

-

P

R

K

K

A

D

L

E

S

I

I

E

L

A

D

S

S

K

Q

G

N

N

S

S

I

H

A

S

G

L

Y

S

Y

L

L

D

D

V

L

H

R

A

N

Q

W

W

L

A

P

T

V

M

M

-

R

-

G

-

A

-

E

E

E

K

G

T

K

G

A

Q

A

W
x
Y

R

F

F

A

T
|
T

P

P

T

V

H

H

V

V

V

I

A

L

A

Q

F

L

L

A

K

E

A

A

L

F

D

R

L

L

H

I

E

E

A

K

E

E

G

-

G

G

V

I

S

E

A

N

R

R

G

I

A

K

R

R

Y

H

T

T

R

M

N

V

R

A

D

S

V

A

V

I

V

R

Q

A

G

G

M

L

R

E

D

A

L

L

G

G

F

L

E

E

T

-

L

I

L

V

A

A

D

R

R

R

-

P

-

E

-

S

W

Y

L

S

S

N

P

T

I

V

I

T

V

G

T

V

F

I

F

L

C

K

P

V

A

A

D

D

P

P

S

Q

F

K

T

V

F

L

Q

A

G

G

F

T

Y

V

D

N

A

-

M

-

K

-

A

-

R

E

G

G

F

V

I

E

I

F

Y

A

P

P

G

G

K

V

L

H

T

P

V

A

V

F

N

K

S

Y

F

F

R

R

I

I

G

G

V

H

I

M

G

G

binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G62 (= G74), T63 (≠ S75), F87 (≠ Y100), T137 (= T151), D162 (= D176), V164 (≠ M178), K188 (= K202), Y239 (≠ W247), T242 (= T250)

3zrpA Crystal structure and substrate specificity of a thermophilic archaeal serine : pyruvate aminotransferase from sulfolobus solfataricus (see paper)
25% identity, 85% coverage: 17:352/393 of query aligns to 1:337/377 of 3zrpA

query
sites
3zrpA

L

L

T

H

P

V

G

G

P

P

L

T

T

T

T

I

A

K

Y

E

S

D

V

V

K

L

Q

V

A

A

M

G

L

L

K

E

D

N

-

N

W

V

G

G

S

F

W

T

D

S

D

K

D

E

F

F

-

V

R

E

A

A

M

L

T

A

R

Y

A

S

L

L

R

K

S

G

R

-

L

-

L

L

A

R

L

Y

I

V

G

M

G

G

G

A

A

S

S

K

D

N

Y

Y

D

Q

C

P

V

L

P

I

I

I

Q

P

G

G

S
x
G

G
|
G

S
x
T

Y

S

C

A

V

M

E

E

A

S

M

V

L

T

G

S

S

L

F

L

V

K

P

P

R

N

D

D

G

-

K

K

V

I

L

L

V

V

L

V

A

S

N

N

G

G

A

V

Y
x
F

G

G

L

D

R

R

A

W

A

E

Q

Q

T

I

L

F

G

K

Y

R

L

Y

E

P

R

V

D

N

Y

V

H

K

L

V

L

L

-

R

-

P

D

S

K

P

G

G

D

D

Y

Y

L

V

P

K

P

P

R

-

G

G

D

E

E

V

V

E

A

E

I

V

L

R

A

K

A

S

D

E

P

Y

A

K

I

L

T

-

H

-

V

V

L

A

A

L

I

T

H

H

C

V

E

E

T
|
T

S

S

S

T

G

G

I

V

Q

R

N

E

P

P

V

V

E

K

E

D

I

V

A

I

E

N

A

K

T

I

K

R

A

K

A

Y

G

V

R

E

K

L

L

I

L

V

I

V

D
|
D

S

G

M
x
V

S

S

A

S

F

V

G

G

A

A

L

E

P

E

L

V

D

K

P

A

V

E

K

E

L

W

N

N

C

V

A

D

A

V

F

Y

V

L

S

T

S

A

A

S

N

Q

K
|
K

C

A

I

L

E

G

G

S

V

A

P

A

G

G

F

L

G

G

F

L

V

L

I

L

A

L

-

S

-

P

R

K

K

A

D

L

E

S

I

I

E

L

A

D

S

S

K

Q

G

N

N

S

S

I

H

A

S

G

L

Y

S

Y

L

L

D

D

V

L

H

R

A

N

Q

W

W

L

A

P

T

V

M

M

-

R

-

G

-

A

-

E

E

E

K

G

T

K

G

A

Q

A

W
x
Y

R

F

F

A

T
|
T

P

P

T

V

H

H

V

V

V

I

A

L

A

Q

F

L

L

A

K

E

A

A

L

F

D

R

L

L

H

I

E

E

A

K

E

E

G

-

G

G

V

I

S

E

A

N

R

R

G

I

A

K

R

R

Y

H

T

T

R

M

N

V

R

A

D

S

V

A

V

I

V

R

Q

A

G

G

M

L

R

E

D

A

L

L

G

G

F

L

E

E

T

-

L

I

L

V

A

A

D

R

R

R

-

P

-

E

-

S

W

Y

L

S

S

N

P

T

I

V

I

T

V

G

T

V

F

I

F

L

C

K

P

V

A

A

D

D

P

P

S

Q

F

K

T

V

F

L

Q

A

G

G

F

T

Y

V

D

N

A

-

M

-

K

-

A

-

R

E

G

G

F

V

I

E

I

F

Y

A

P

P

G

G

K

V

L

H

T

P

V

A

V

F

N

K

S

Y

F

F

R

R

I

I

G

G

V

H

I

M

G

G

binding pyridoxal-5'-phosphate: G57 (≠ S73), G58 (= G74), T59 (≠ S75), F83 (≠ Y100), T133 (= T151), D158 (= D176), V160 (≠ M178), K184 (= K202), Y235 (≠ W247), T238 (= T250)

1vjoA Crystal structure of alanine--glyoxylate aminotransferase (alr1004) from nostoc sp. At 1.70 a resolution (see paper)
24% identity, 76% coverage: 16:314/393 of query aligns to 21:319/377 of 1vjoA

query
sites
1vjoA

L

L

T

G

P

P

G

G

P

P

L

S

T

N

T

A

A

H

Y

P

S

S

V

V

K

L

Q

Q

A

A

M

M

-

N

L

V

K

S

D

P

W

V

G

G

S

H

W

L

D

D

D

P

D

A

F

F

R

L

A

A

M

L

T

M

R

D

A

E

L

I

R

Q

S

S

R

L

L

L

L

R

A

Y

L

V

I

W

G

Q

G

-

A

T

S

E

D

N

Y

P

D

L

C

T

V

I

P

A

I

V

Q

S

G

G

S
x
T

G
|
G

S
x
T

Y

A

C

A

V

M

E

E

A

A

M

T

L

I

G

A

S

N

F

A

V

V

P

E

R

P

D

G

G

D

K

V

V

V

L

L

V

I

L

G

A

V

N

A

G

G

A

Y

Y
x
F

G

G

L

N

R

R

A

L

A

V

Q

D

T

M

L

A

G

G

Y

R

L

Y

E

G

R

A

D

D

Y

V

H

R

L

T

L

I

D

S

K

K

G

-

D

-

Y

-

L

-

P

-

P

P

R

W

G

G

D

E

E

V

V

F

A

S

-

L

-

E

-

L

-

R

-

T

A

A

I

L

L

E

A

T

A

H

D

R

P

P

A

A

I

I

T

L

H

A

V

L

L

-

A

-

I

V

H

H

C

A

E

E

T
|
T

S

S

S

T

G

G

I

A

Q

R

N

Q

P

P

V

L

E

E

E

G

I

V

A

G

E

E

A

L

T

C

K

R

A

E

A

F

G

G

R

T

K

L

L

L

I

V

D
|
D

S

T

M
x
V

S

T

A

S

F

L

G

G

A

G

L

V

P

P

-

I

-

F

L

L

D

D

P

A

V

W

K

G

L

V

N

D

C

L

A

A

A

Y

F

S

V

C

S

S

S

Q

A
x
K

N

G

-

L

K

G

C

C

I

S

E

P

G

G

V

A

P

S

G

P

F

F

G

T

F

M

V

S

I

S

A

R

R

A

K

I

D

E

E

K

I

L

E

Q

A

R

S

R

K

R

G

T

N

K

S

V

H

A

S

N

L

W

S

Y

L

L

D

D

V

M

H

N

A

L

Q

L

W

G

A

K

T

Y

M

W

E

G

K

S

T

E

G

R

Q

V

W

Y

R

H

F

H

T

T

P

A

P

P

T

I

H

N

V

L

V

Y

A

Y

A

A

F

L

L

R

K

E

A

A

L

L

D

R

L

L

H

I

E

-

A

A

E

Q

G

E

G

G

V

L

S

A

A

N

R

C

G

W

A

Q

R

R

Y

H

T

Q

R

K

N

N

R

V

D

E

V

Y

V

L

V

W

Q

E

G

R

M

L

R

E

D

D

L

I

G

G

F

L

E

S

T

L

L

H

L

V

A

E

D

K

R

E

W

Y

L

R

S

L

P

P

I

T

I

L

V

T

T

T

F

V

F

C

C

I

P

P

binding pyridoxal-5'-phosphate: T77 (≠ S73), G78 (= G74), T79 (≠ S75), F104 (≠ Y100), T154 (= T151), D179 (= D176), V181 (≠ M178), K205 (≠ A200)

Q56YA5 Serine--glyoxylate aminotransferase; Alanine--glyoxylate aminotransferase; AGT; Asparagine aminotransferase; Serine--pyruvate aminotransferase; EC 2.6.1.45; EC 2.6.1.44; EC 2.6.1.-; EC 2.6.1.51 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
22% identity, 88% coverage: 12:356/393 of query aligns to 8:356/401 of Q56YA5

query
sites
Q56YA5

G

G

E

R

P

H

Y

H

L

L

L

F

T

V

P

P

G

G

P

P

L

V

T

N

T

I

A

P

Y

E

S

P

V

V

K

I

Q

R

A

A

M

M

L

N

K

R

D

N

W

-

G

-

S

-

W

-

D

N

D

E

D

D

F

Y

R

R

-

S

-

P

-

A

-

I

-

P

A

A

M

L

T

T

R

K

A

T

L

L

R

L

S

E

R

D

L

V

L

K

A

K

L

I

I

F

G

K

G

T

A

S

S

G

D

T

Y

P

D

F

C

L

V

F

P

P

I

T

Q
x
T

G
|
G

S
x
T

G

G

S

A

Y

W

C

-

V

E

E

S

A

A

M

L

L

T

G

N

S

T

F

L

V

S

P

P

R

G

D

D

G

-

K

R

V

I

L

V

V

S

L

F

A

L

N

I

G

G

A

Q

Y

F

G

S

L

L

R

L

A

W

A

I

Q

D

T

Q

L

Q

G

K

Y

R

L

L

E

N

R

F

D

N

Y

V

H

D

L

V

D

E

K

S

G

D

D

W

Y

G

L

Q

P

G

P

A

R

N

G

L

D

Q

E

V

V

L

A

A

A

S

I

K

L

L

A

S

A

Q

D

D

P

E

-

N

-

H

A

T

I

I

T

K

H

A

V

I

L

C

A

I

I

V

H

H

C

N

E

E

T
|
T

S

A

S

T

G

G

I

V

Q

T

N

N

P

D

V

I

E

S

E

A

I

V

A

R

E

T

A

L

T

L

K

D

A

H

A

Y

G

K

R

H

K

P

-

A

-

L

L

L

I

V

D

D

S

G

M

V

S

S

A

S

F

I

G

C

A

A

L

L

P

D

L

F

D

R

P

M

V

D

K

E

L

W

N

G

C

V

A

D

A

V

F

A

V

L

S

T

S

G

A

S

N
x
Q

K
|
K

C

A

I

L

E

S

G

L

V

P

P

T

G

G

F

L

G

G

F

I

V

V

I

C

A

A

R

S

K

P

D

K

E

A

I

L

E

E

A

A

S

T

K

K

G

T

N

-

S

-

H

-

S

S

L

K

S

S

L

L

D

K

V

V

H

F

A

F

Q

D

W

W

A

N

T

D

M

Y

E

L

K

K

T

F

G

Y

Q

K

-

L

-

G

-

T

-

Y

W

W

R

P

F

Y

T

T

P
|
P

P

S

T

I

H

Q

V

L

A

Y

A

G

F

L

L

R

K

A

A

A

L

L

D

D

L

L

H

I

E

F

A

E

E

E

G

G

-

L

-

E

G

N

V

I

S

I

A

A

R

R

G

H

A

A

R

R

Y

L

T

G

R

K

N

A

R

T

D

R

V

L

V

A

V

V

Q

E

-

A

-

W

G

G

M

L

R

K

D

N

L

C

G

T

F

Q

E

K

T

-

L

-

A

-

D

E

R

E

W

W

L

I

S

S

P

N

I

T

I

V

V

T

T

A

F

V

F

M

C

V

P

P

-

P

-

H

A

I

D

D

P

G

S

S

F

E

T

I

F

V

Q

R

-

R

-

A

-

W

G

Q

F

R

Y

Y

D

N

A

L

M

S

K

L

A

G

R

L

G

G

F

L

I

N

I

K

Y

V

P

A

G

G

K

K

L

V

T

-

V

-

N

-

S

-

F

F

R
|
R

I

I

G

G

V

H

I

L

G

G

Q

N

M

V

D

N

E

E

TGT 68:70 (≠ QGS 71:73) binding pyridoxal 5'-phosphate
T148 (= T151) binding pyridoxal 5'-phosphate
QK 200:201 (≠ NK 201:202) binding pyridoxal 5'-phosphate
K201 (= K202) binding 3-hydroxypyruvate
P251 (= P251) mutation to L: Abolishes aminotransferase activity.
R347 (= R347) binding 3-hydroxypyruvate

6pk3B Alanine-glyoxylate aminotransferase 1 (agt1) from arabidopsis thaliana (see paper)
22% identity, 88% coverage: 12:356/393 of query aligns to 7:355/400 of 6pk3B

query
sites
6pk3B

G

G

E

R

P

H

Y

H

L

L

L

F

T

V

P

P

G

G

P

P

L

V

T

N

T

I

A

P

Y

E

S

P

V

V

K

I

Q

R

A

A

M

M

L

N

K

R

D

N

W

-

G

-

S

-

W

-

D

N

D

E

D

D

F

Y

R

R

-

S

-

P

-

A

-

I

-

P

A

A

M

L

T

T

R

K

A

T

L

L

R

L

S

E

R

D

L

V

L

K

A

K

L

I

I

F

G

K

G

T

A

S

S

G

D

T

Y

P

D

F

C

L

V

F

P

P

I

T

Q
x
T

G
|
G

S
x
T

G

G

S

A

Y
x
W

C

-

V

E

E

S

A

A

M

L

L

T

G

N

S

T

F

L

V

S

P

P

R

G

D

D

G

-

K

R

V

I

L

V

V

S

L

F

A

L

N

I

G

G

A

Q

Y
x
F

G

S

L

L

R

L

A

W

A

I

Q

D

T

Q

L

Q

G

K

Y

R

L

L

E

N

R

F

D

N

Y

V

H

D

L

V

D

E

K

S

G

D

D

W

Y

G

L

Q

P

G

P

A

R

N

G

L

D

Q

E

V

V

L

A

A

A

S

I

K

L

L

A

S

A

Q

D

D

P

E

-

N

-

H

A

T

I

I

T

K

H

A

V

I

L

C

A

I

I

V

H

H

C

N

E

E

T
|
T

S

A

S

T

G

G

I

V

Q

T

N

N

P

D

V

I

E

S

E

A

I

V

A

R

E

T

A

L

T

L

K

D

A

H

A

Y

G

K

R

H

K

P

-

A

-

L

L

L

I

V

D
|
D

S

G

M
x
V

S

S

A

S

F

I

G

C

A

A

L

L

P

D

L

F

D

R

P

M

V

D

K

E

L

W

N

G

C

V

A

D

A

V

F

A

V

L

S

T

S

G

A

S

N
x
Q

K
|
K

C

A

I

L

E

S

G

L

V

P

P

T

G

G

F

L

G

G

F

I

V

V

I

C

A

A

R

S

K

P

D

K

E

A

I

L

E

E

A

A

S

T

K

K

G

T

N

-

S

-

H

-

S

S

L

K

S

S

L

L

D

K

V

V

H

F

A

F

Q

D

W

W

A

N

T

D

M

Y

E

L

K

K

T

F

G

Y

Q

K

-

L

-

G

-

T

-

Y

W

W

R

P

F

Y

T

T

P

P

P

S

T

I

H

Q

V

L

A

Y

A

G

F

L

L

R

K

A

A

A

L

L

D

D

L

L

H

I

E

F

A

E

E

E

G

G

-

L

-

E

G

N

V

I

S

I

A

A

R

R

G

H

A

A

R

R

Y

L

T

G

R

K

N

A

R

T

D

R

V

L

V

A

V

V

Q

E

-

A

-

W

G

G

M

L

R

K

D

N

L

C

G

T

F

Q

E

K

T

-

L

-

A

-

D

E

R

E

W

W

L

I

S

S

P

N

I

T

I

V

V

T

T

A

F

V

F

M

C

V

P

P

-

P

-

H

A

I

D

D

P

G

S

S

F

E

T

I

F

V

Q

R

-

R

-

A

-

W

G

Q

F

R

Y

Y

D

N

A

L

M

S

K

L

A

G

R

L

G

G

F

L

I

N

I

K

Y

V

P

A

G

G

K

K

L

V

T

-

V

-

N

-

S

-

F

F

R

R

I

I

G

G

V

H

I

L

G

G

Q

N

M

V

D

N

E

E

binding pyridoxal-5'-phosphate: T67 (≠ Q71), G68 (= G72), T69 (≠ S73), W72 (≠ Y76), F94 (≠ Y100), T147 (= T151), D174 (= D176), V176 (≠ M178), Q199 (≠ N201), K200 (= K202)

6pk1A Alanine-glyoxylate aminotransferase 1 (agt1) from arabidopsis thaliana in presence of serine (see paper)
22% identity, 88% coverage: 12:356/393 of query aligns to 6:354/399 of 6pk1A

query
sites
6pk1A

G

G

E

R

P

H

Y

H

L

L

L

F

T

V

P

P

G

G

P

P

L

V

T

N

T

I

A

P

Y

E

S

P

V

V

K

I

Q

R

A

A

M

M

L

N

K

R

D

N

W

-

G

-

S

-

W

-

D

N

D

E

D

D

F

Y

R

R

-

S

-

P

-

A

-

I

-

P

A

A

M

L

T

T

R

K

A

T

L

L

R

L

S

E

R

D

L

V

L

K

A

K

L

I

I

F

G

K

G

T

A

S

S

G

D

T

Y

P

D

F

C

L

V

F

P

P

I

T

Q
x
T

G
|
G

S
x
T

G

G

S

A

Y
x
W

C

-

V

E

E

S

A

A

M

L

L

T

G

N

S

T

F

L

V

S

P

P

R

G

D

D

G

-

K

R

V

I

L

V

V

S

L

F

A

L

N

I

G

G

A

Q

Y
x
F

G

S

L

L

R

L

A

W

A

I

Q

D

T

Q

L

Q

G

K

Y

R

L

L

E

N

R

F

D

N

Y

V

H

D

L

V

D

E

K

S

G

D

D

W

Y

G

L

Q

P

G

P

A

R

N

G

L

D

Q

E

V

V

L

A

A

A

S

I

K

L

L

A

S

A

Q

D

D

P

E

-

N

-

H

A

T

I

I

T

K

H

A

V

I

L

C

A

I

I

V

H

H

C

N

E

E

T
|
T

S

A

S

T

G

G

I

V

Q

T

N

N

P

D

V

I

E

S

E

A

I

V

A

R

E

T

A

L

T

L

K

D

A

H

A

Y

G

K

R

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binding 3-hydroxypyruvic acid: F93 (≠ Y100), R345 (= R347)
binding pyridoxal-5'-phosphate: T66 (≠ Q71), G67 (= G72), T68 (≠ S73), W71 (≠ Y76), F93 (≠ Y100), T146 (= T151), D173 (= D176), V175 (≠ M178), K199 (= K202)

P21549 Alanine--glyoxylate aminotransferase; AGT; Serine--pyruvate aminotransferase; SPT; EC 2.6.1.44; EC 2.6.1.51 from Homo sapiens (Human) (see 24 papers)
25% identity, 77% coverage: 70:370/393 of query aligns to 78:384/392 of P21549

query
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G82 (= G74) to E: in HP1; abolishes alanine--glyoxylate aminotransferase activity by interfering with pyridoxal phosphate binding; dbSNP:rs121908522
W108 (≠ Y100) to R: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; dbSNP:rs180177197
A112 (= A104) to D: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; causes protein aggregation; dbSNP:rs796052061
L150 (≠ T142) to P: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177222
F152 (≠ V144) to I: in HP1; associated in cis with L-11 and M-340; results in protein destabilization; no loss of dimerization; decreased alanine--glyoxylate aminotransferase activity; loss of alanine--glyoxylate aminotransferase activity when associated with L-11 and M-340; mitochondrial mistargeting when associated with L-11 and M-340; dbSNP:rs121908524
G156 (≠ C149) to R: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; dbSNP:rs121908530
S158 (≠ T151) to L: in HP1; loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177225
G161 (= G154) to C: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; reduced expression levels; decreased protein stability; protein aggregation seen in the cytosol with a decreased aggregation propensity in the presence of pyridoxal phosphate; reduced peroxisomal localization; dbSNP:rs180177227; to R: in HP1; loss of alanine--glyoxylate aminotransferase activity; reduced expression levels; decreased protein stability; protein aggregation seen in the cytosol with a decreased aggregation propensity in the presence of pyridoxal phosphate; loss of dimerization; dbSNP:rs180177227; to S: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; reduced expression levels; decreased protein stability; protein aggregation seen in the cytosol with a decreased aggregation propensity in the presence of pyridoxal phosphate; reduced peroxisomal localization; dbSNP:rs180177227
L166 (≠ V159) to P: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177230
G170 (≠ A163) to R: in HP1; associated in cis with L-11 and M-340; decrease in alanine--glyoxylate aminotransferase activity; loss of dimerization; partial loss of protein stability but protein stability increases in the presence of pyridoxal phosphate; causes protein aggregation; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with L-11 and M-340; results in mitochondrial mistargeting when associated with L-11 and M-340; dbSNP:rs121908529
C173 (≠ T166) to Y: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; causes protein aggregation; dbSNP:rs180177231
D183 (= D176) to N: in HP1; loss of alanine--glyoxylate aminotransferase activity; no loss of dimerization; no effect on protein stability; dbSNP:rs180177236
S187 (≠ A180) to F: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization but improved dimerization in the presence of pyridoxal phosphate; decreased protein stability; dbSNP:rs180177238
I202 (≠ A195) to N: in HP1; uncertain significance; dbSNP:rs536352238
S205 (= S198) to P: in HP1; loss of alanine--glyoxylate aminotransferase activity; decreased protein stability; dbSNP:rs121908520
K209 (= K202) mutation to R: Affects pyridoxal phosphate binding; loss of alanine--glyoxylate aminotransferase activity.
S218 (vs. gap) to L: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; no effect on protein stability; dbSNP:rs180177253
R233 (≠ S223) to C: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs121908526; to H: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs121908527
I244 (≠ V234) to T: in HP1; associated in cis with L-11 and M-340; results in protein misfolding; decreased alanine--glyoxylate aminotransferase activity in vitro; no loss of dimerization; partial mitochondrial mistargeting; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with L-11 and M-340; dbSNP:rs121908525
C253 (≠ T240) to R: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177264
I279 (≠ E267) to T: in dbSNP:rs140992177
A280 (= A268) to V: in dbSNP:rs73106685
V326 (≠ C313) to I: in dbSNP:rs115057148
I340 (= I335) to M: in allele minor; associated in cis with L-11; no effect on alanine--glyoxylate aminotransferase activity in vitro; decreased specific alanine--glyoxylate aminotransferase activity in vitro when associated with L-11; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with L-11 and I-152; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with L-11 and R-170; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with L-11 and T-244; results in mitochondrial mistargeting when associated with L-11 and R-170; dbSNP:rs4426527

Sites not aligning to the query:

9 T → N: no loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs115014558
11 P → L: in allele minor; associated in cis with M-340; decreased specific alanine--glyoxylate aminotransferase activity in vitro when associated with M-340; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with I-152 and M-340; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with R-170 and M-340; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with T-244 and M-340; causes mitochondrial mistargeting when associated with R-170 and M-340; dbSNP:rs34116584
36 R → C: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177157
41 G → E: in HP1; loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177168; G → R: in HP1; associated in cis with L-11 and M-340; results in loss of protein stability; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; partial mitochondrial mistargeting; intraperoxisomal protein aggregation seen; dbSNP:rs121908523; G → V: in HP1; reduced alanine--glyoxylate aminotransferase activity; no loss of dimerization; no effect on protein stability; dbSNP:rs180177168
47 G → R: in HP1; associated in cis with L-11 and M-340; results in protein misfolding; decreased alanine--glyoxylate aminotransferase activity; reduced expression levels; reduced pyridoxal phosphate binding; reduced dimerization; reduced thermostability; increased propensity to aggregation; increased susceptibility to proteolytic degradation within the N-terminal region; mitochondrial mistargeting; exposure to pyridoxine can rescue the functionality by partially preventing aggregation and degradation and by redirecting all the protein to the peroxisome; dbSNP:rs180177173

Query Sequence

>WP_085635109.1 NCBI__GCF_002115805.1:WP_085635109.1
MSATPLPAPELGEPYLLTPGPLTTAYSVKQAMLKDWGSWDDDFRAMTRALRSRLLALIGG
GASDYDCVPIQGSGSYCVEAMLGSFVPRDGKVLVLANGAYGLRAAQTLGYLERDYHLLDK
GDYLPPRGDEVAAILAADPAITHVLAIHCETSSGIQNPVEEIAEATKAAGRKLLIDSMSA
FGALPLDPVKLNCAAFVSSANKCIEGVPGFGFVIARKDEIEASKGNSHSLSLDVHAQWAT
MEKTGQWRFTPPTHVVAAFLKALDLHEAEGGVSARGARYTRNRDVVVQGMRDLGFETLLA
DRWLSPIIVTFFCPADPSFTFQGFYDAMKARGFIIYPGKLTVVNSFRIGVIGQMDEHVMR
RVVEAARDALTDMNVSSAAPPAAALEERKKLAA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory