SitesBLAST
Comparing WP_085635633.1 NCBI__GCF_002115805.1:WP_085635633.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
3jz4A Crystal structure of e. Coli NADP dependent enzyme (see paper)
45% identity, 98% coverage: 8:472/476 of query aligns to 14:477/481 of 3jz4A
- active site: N156 (= N150), K179 (= K173), E254 (= E249), C288 (= C283), E385 (= E380), E462 (= E457)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: P154 (= P148), W155 (= W149), K179 (= K173), A181 (= A175), S182 (= S176), A212 (≠ T207), G216 (≠ V211), G232 (= G227), S233 (= S228), I236 (≠ V231), C288 (= C283), K338 (= K333), E385 (= E380), F387 (= F382)
P25526 Succinate-semialdehyde dehydrogenase [NADP(+)] GabD; SSDH; Glutarate-semialdehyde dehydrogenase; EC 1.2.1.79; EC 1.2.1.- from Escherichia coli (strain K12) (see paper)
45% identity, 98% coverage: 8:472/476 of query aligns to 15:478/482 of P25526
8of1A Structure of aldh5f1 from moss physcomitrium patens in complex with NAD+ in the contracted conformation
44% identity, 98% coverage: 8:472/476 of query aligns to 32:495/505 of 8of1A
- binding nicotinamide-adenine-dinucleotide: I170 (≠ V146), A171 (≠ T147), P172 (= P148), W173 (= W149), K197 (= K173), A230 (≠ R206), F248 (= F225), G250 (= G227), S251 (= S228), V254 (= V231), M257 (≠ K234), L273 (= L250), C306 (= C283), K356 (= K333), E403 (= E380), F405 (= F382)
8c54A Cryo-em structure of nadh bound sla dehydrogenase rlgabd from rhizobium leguminosarum bv. Trifolii srd1565 (see paper)
43% identity, 98% coverage: 7:473/476 of query aligns to 13:478/482 of 8c54A
- binding 1,4-dihydronicotinamide adenine dinucleotide: I152 (≠ V146), T153 (= T147), P154 (= P148), K179 (= K173), A212 (≠ T207), K213 (≠ G208), F230 (= F225), T231 (= T226), G232 (= G227), S233 (= S228), V236 (= V231), W239 (≠ K234), G256 (= G251)
P51649 Succinate-semialdehyde dehydrogenase, mitochondrial; Aldehyde dehydrogenase family 5 member A1; NAD(+)-dependent succinic semialdehyde dehydrogenase; EC 1.2.1.24 from Homo sapiens (Human) (see 5 papers)
43% identity, 99% coverage: 4:473/476 of query aligns to 61:531/535 of P51649
- C93 (≠ A38) to F: in SSADHD; 3% of activity; dbSNP:rs765561257
- G176 (= G121) to R: in SSADHD; <1% of activity; dbSNP:rs72552281
- H180 (= H125) to Y: 83% of activity; dbSNP:rs2760118
- P182 (= P127) to L: 48% of activity; dbSNP:rs3765310
- R213 (= R158) binding substrate; mutation to A: Reduces catalytic activity to less than 15% of wild-type.
- C223 (= C168) to Y: in SSADHD; 5% of activity; dbSNP:rs72552282
- KPAE 228:231 (≠ KPAS 173:176) binding NAD(+)
- T233 (= T178) to M: in SSADHD; 4% of activity; dbSNP:rs1326526453
- A237 (≠ M182) to S: 65% of activity; dbSNP:rs62621664
- N255 (= N200) to S: in SSADHD; 17% of activity; dbSNP:rs145087265
- G268 (≠ V211) to E: in SSADHD; <1% of activity; dbSNP:rs375628463
- GSTTTG 284:289 (≠ GSTGVG 227:232) binding NAD(+)
- R334 (= R277) binding substrate; mutation to A: Reduces catalytic activity to less than 15% of wild-type.
- N335 (= N278) to K: in SSADHD; 1% of activity; dbSNP:rs72552283
- C340 (= C283) modified: Disulfide link with 342, In inhibited form
- C342 (≠ A285) modified: Disulfide link with 340, In inhibited form; mutation to A: Loss of regulation by redox state.
- N372 (≠ D314) natural variant: N -> S
- P382 (= P324) to L: in SSADHD; 2% of activity
- V406 (≠ I348) to I: in dbSNP:rs143741652
- G409 (= G351) to D: in SSADHD; <1% of activity; dbSNP:rs118203984
- S498 (= S440) binding substrate; mutation to A: Reduces catalytic activity to less than 15% of wild-type.
Sites not aligning to the query:
- 36 G → R: no effect on succinate-semialdehyde dehydrogenase activity; dbSNP:rs4646832
- 533 G → R: in SSADHD; <1% of activity; dbSNP:rs72552284
2w8rA The crystal structure of human ssadh in complex with NAD+ (see paper)
43% identity, 99% coverage: 4:472/476 of query aligns to 11:480/485 of 2w8rA
2w8qA The crystal structure of human ssadh in complex with ssa. (see paper)
43% identity, 99% coverage: 4:472/476 of query aligns to 11:480/485 of 2w8qA
6j76A Structure of 3,6-anhydro-l-galactose dehydrogenase in complex with nap (see paper)
37% identity, 98% coverage: 6:473/476 of query aligns to 4:476/477 of 6j76A
- active site: N148 (= N150), E246 (= E249), C280 (= C283), E458 (= E457)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: I144 (≠ V146), T145 (= T147), A146 (≠ P148), W147 (= W149), N148 (= N150), K171 (= K173), T173 (≠ A175), S174 (= S176), G204 (≠ K205), G208 (vs. gap), T223 (= T226), G224 (= G227), S225 (= S228), A228 (≠ V231), S231 (≠ K234), I232 (≠ L235), E246 (= E249), L247 (= L250), C280 (= C283), E381 (= E380), F383 (= F382), H447 (≠ F446)
4pz2B Structure of zm aldh2-6 (rf2f) in complex with NAD (see paper)
40% identity, 99% coverage: 3:472/476 of query aligns to 10:484/494 of 4pz2B
- active site: N159 (= N150), K182 (= K173), E258 (= E249), C292 (= C283), E392 (= E380), D469 (≠ E457)
- binding nicotinamide-adenine-dinucleotide: I155 (≠ V146), I156 (≠ T147), P157 (= P148), W158 (= W149), N159 (= N150), M164 (= M155), K182 (= K173), A184 (= A175), E185 (≠ S176), G215 (vs. gap), G219 (≠ T207), F233 (= F225), T234 (= T226), G235 (= G227), S236 (= S228), V239 (= V231), E258 (= E249), L259 (= L250), C292 (= C283), E392 (= E380), F394 (= F382)
4neaA 1.90 angstrom resolution crystal structure of betaine aldehyde dehydrogenase (betb) from staphylococcus aureus in complex with NAD+ and bme-free cys289 (see paper)
35% identity, 98% coverage: 7:472/476 of query aligns to 21:491/505 of 4neaA