SitesBLAST
Comparing WP_085637757.1 NCBI__GCF_002115805.1:WP_085637757.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
8hprD Lpqy-sugabc in state 4 (see paper)
51% identity, 95% coverage: 20:365/365 of query aligns to 19:360/362 of 8hprD
- binding adenosine-5'-triphosphate: S38 (= S39), C40 (= C41), G41 (= G42), K42 (= K43), S43 (= S44), T44 (= T45), Q82 (= Q83), R129 (= R130), Q133 (= Q134), S135 (= S136), G136 (= G137), G137 (= G138), Q159 (≠ E160), H192 (= H193)
- binding magnesium ion: S43 (= S44), Q82 (= Q83)
Sites not aligning to the query:
8hprC Lpqy-sugabc in state 4 (see paper)
51% identity, 95% coverage: 20:365/365 of query aligns to 19:361/363 of 8hprC
- binding adenosine-5'-triphosphate: S38 (= S39), G39 (= G40), G41 (= G42), K42 (= K43), S43 (= S44), Q82 (= Q83), Q133 (= Q134), G136 (= G137), G137 (= G138), Q138 (= Q139), H192 (= H193)
- binding magnesium ion: S43 (= S44), Q82 (= Q83)
Sites not aligning to the query:
8hplC Lpqy-sugabc in state 1 (see paper)
48% identity, 98% coverage: 7:365/365 of query aligns to 5:382/384 of 8hplC
P9WQI3 Trehalose import ATP-binding protein SugC; MtbSugC; Nucleotide-binding domain of SugABC transporter; NBD of SugABC transporter; SugABC transporter ATPase SugC; EC 7.5.2.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
48% identity, 99% coverage: 4:365/365 of query aligns to 5:388/393 of P9WQI3
- H193 (= H193) mutation to A: Decreased hydrolysis of ATP. No change in KM, but 2-fold reduction in Vmax compared to wild-type.
2d62A Crystal structure of multiple sugar binding transport atp-binding protein
43% identity, 96% coverage: 9:360/365 of query aligns to 11:370/375 of 2d62A
1g291 Malk (see paper)
44% identity, 97% coverage: 9:361/365 of query aligns to 8:368/372 of 1g291
- binding magnesium ion: D69 (vs. gap), E71 (vs. gap), K72 (vs. gap), K79 (= K74), D80 (= D75), E292 (= E287), D293 (= D288), K359 (= K352)
- binding pyrophosphate 2-: S38 (= S39), G39 (= G40), C40 (= C41), G41 (= G42), K42 (= K43), T43 (≠ S44), T44 (= T45)
2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
50% identity, 80% coverage: 5:296/365 of query aligns to 3:301/374 of 2awnB
P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
50% identity, 80% coverage: 5:296/365 of query aligns to 4:300/369 of P19566
- L86 (= L87) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- P160 (= P161) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- D165 (= D166) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
Sites not aligning to the query:
- 306 E→K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.
3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
50% identity, 80% coverage: 5:296/365 of query aligns to 3:301/371 of 3puyA
- binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ Y14), S37 (= S39), G38 (= G40), C39 (= C41), G40 (= G42), K41 (= K43), S42 (= S44), T43 (= T45), Q81 (= Q83), R128 (= R130), A132 (≠ Q134), S134 (= S136), G136 (= G138), Q137 (= Q139), E158 (= E160), H191 (= H193)
- binding magnesium ion: S42 (= S44), Q81 (= Q83)
3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
50% identity, 80% coverage: 5:296/365 of query aligns to 3:301/371 of 3puxA
- binding adenosine-5'-diphosphate: W12 (≠ Y14), G38 (= G40), C39 (= C41), G40 (= G42), K41 (= K43), S42 (= S44), T43 (= T45), R128 (= R130), S134 (= S136), Q137 (= Q139)
- binding beryllium trifluoride ion: S37 (= S39), G38 (= G40), K41 (= K43), Q81 (= Q83), S134 (= S136), G136 (= G138), H191 (= H193)
- binding magnesium ion: S42 (= S44), Q81 (= Q83)
3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
50% identity, 80% coverage: 5:296/365 of query aligns to 3:301/371 of 3puwA
- binding adenosine-5'-diphosphate: W12 (≠ Y14), V17 (≠ I19), G38 (= G40), C39 (= C41), G40 (= G42), K41 (= K43), S42 (= S44), T43 (= T45), R128 (= R130), A132 (≠ Q134), S134 (= S136), Q137 (= Q139)
- binding tetrafluoroaluminate ion: S37 (= S39), G38 (= G40), K41 (= K43), Q81 (= Q83), S134 (= S136), G135 (= G137), G136 (= G138), E158 (= E160), H191 (= H193)
- binding magnesium ion: S42 (= S44), Q81 (= Q83)
3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
50% identity, 80% coverage: 5:296/365 of query aligns to 3:301/371 of 3puvA
- binding adenosine-5'-diphosphate: W12 (≠ Y14), V17 (≠ I19), G38 (= G40), C39 (= C41), G40 (= G42), K41 (= K43), S42 (= S44), T43 (= T45), R128 (= R130), A132 (≠ Q134), S134 (= S136), Q137 (= Q139)
- binding magnesium ion: S42 (= S44), Q81 (= Q83)
P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
50% identity, 80% coverage: 5:296/365 of query aligns to 4:302/371 of P68187
- A85 (= A86) mutation to M: Suppressor of EAA loop mutations in MalFG.
- K106 (≠ D107) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V114 (≠ L115) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V117 (= V118) mutation to M: Suppressor of EAA loop mutations in MalFG.
- E119 (≠ Q120) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- A124 (≠ E125) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G137 (= G138) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
- D158 (= D159) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
- R228 (≠ L229) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- F241 (≠ L242) mutation to I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- W267 (≠ V267) mutation to G: Normal maltose transport but constitutive mal gene expression.
- G278 (≠ P277) mutation to P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- S282 (≠ I281) mutation to L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G284 (= G283) mutation to S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G302 (= G296) mutation to D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
Sites not aligning to the query:
- 308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
- 322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 340 G→A: Maltose transport is affected but retains ability to interact with MalT.
- 346 G→S: Normal maltose transport but constitutive mal gene expression.
- 355 F→Y: Maltose transport is affected but retains ability to interact with MalT.
1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
50% identity, 80% coverage: 5:296/365 of query aligns to 1:299/367 of 1q12A