SitesBLAST
Comparing WP_085639734.1 NCBI__GCF_002115805.1:WP_085639734.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P22256 4-aminobutyrate aminotransferase GabT; 5-aminovalerate transaminase; GABA aminotransferase; GABA-AT; Gamma-amino-N-butyrate transaminase; GABA transaminase; Glutamate:succinic semialdehyde transaminase; L-AIBAT; EC 2.6.1.19; EC 2.6.1.48 from Escherichia coli (strain K12) (see 2 papers)
54% identity, 92% coverage: 26:450/460 of query aligns to 2:424/426 of P22256
- I50 (= I74) mutation to Q: 3-fold decrease in catalytic activity and 12-fold decrease in affinity for GABA.
- GS 111:112 (≠ GA 135:136) binding pyridoxal 5'-phosphate
- E211 (= E235) mutation to S: 100-fold decrease in catalytic activity and 15-fold decrease in affinity for GABA.
- V241 (≠ I265) mutation to A: 25-fold decrease in catalytic activity and 5-fold decrease in affinity for GABA.
- Q242 (= Q266) binding pyridoxal 5'-phosphate
- K268 (= K292) modified: N6-(pyridoxal phosphate)lysine
- T297 (= T321) binding pyridoxal 5'-phosphate
1sffA Structure of gamma-aminobutyrate aminotransferase complex with aminooxyacetate (see paper)
54% identity, 92% coverage: 26:450/460 of query aligns to 1:423/425 of 1sffA
- active site: V18 (= V43), Y137 (= Y162), E205 (= E230), D238 (= D263), Q241 (= Q266), K267 (= K292), T296 (= T321), R397 (= R424)
- binding 4'-deoxy-4'-acetylyamino-pyridoxal-5'-phosphate: Q78 (= Q103), G110 (= G135), S111 (≠ A136), Y137 (= Y162), H138 (= H163), R140 (= R165), E205 (= E230), D238 (= D263), V240 (≠ I265), Q241 (= Q266), K267 (= K292), T296 (= T321)
- binding sulfate ion: N152 (= N177), Y393 (≠ G420)
1sf2A Structure of e. Coli gamma-aminobutyrate aminotransferase (see paper)
54% identity, 92% coverage: 26:450/460 of query aligns to 1:423/425 of 1sf2A
- active site: V18 (= V43), Y137 (= Y162), E205 (= E230), D238 (= D263), Q241 (= Q266), K267 (= K292), T296 (= T321), R397 (= R424)
- binding pyridoxal-5'-phosphate: G110 (= G135), S111 (≠ A136), Y137 (= Y162), H138 (= H163), E205 (= E230), D238 (= D263), V240 (≠ I265), Q241 (= Q266), K267 (= K292)
- binding sulfate ion: N152 (= N177), Y393 (≠ G420)
1szkA The structure of gamma-aminobutyrate aminotransferase mutant: e211s (see paper)
54% identity, 92% coverage: 26:450/460 of query aligns to 1:423/425 of 1szkA
- active site: V18 (= V43), Y137 (= Y162), E205 (= E230), D238 (= D263), Q241 (= Q266), K267 (= K292), T296 (= T321), R397 (= R424)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G110 (= G135), S111 (≠ A136), Y137 (= Y162), H138 (= H163), E205 (= E230), D238 (= D263), V240 (≠ I265), Q241 (= Q266), K267 (= K292)
P50457 4-aminobutyrate aminotransferase PuuE; GABA aminotransferase; GABA-AT; Gamma-amino-N-butyrate transaminase; GABA transaminase; Glutamate:succinic semialdehyde transaminase; EC 2.6.1.19 from Escherichia coli (strain K12) (see paper)
53% identity, 92% coverage: 28:449/460 of query aligns to 3:421/421 of P50457
- K267 (= K292) mutation to A: No GABA-AT activity.
3q8nC Crystal structure of 4-aminobutyrate transaminase from mycobacterium smegmatis (see paper)
48% identity, 91% coverage: 31:449/460 of query aligns to 20:437/439 of 3q8nC
- active site: V32 (= V43), Y151 (= Y162), E221 (= E230), D254 (= D263), Q257 (= Q266), K283 (= K292), T312 (= T321), R412 (= R424)
- binding 4-oxobutanoic acid: G124 (= G135), A125 (= A136), V256 (≠ I265), K283 (= K292)
4atqF Gaba-transaminase a1r958 in complex with external aldimine plp-gaba adduct (see paper)
47% identity, 91% coverage: 30:447/460 of query aligns to 22:441/444 of 4atqF
- active site: V35 (= V43), Y154 (= Y162), E226 (= E230), D259 (= D263), Q262 (= Q266), K288 (= K292), T317 (= T321), R418 (= R424)
- binding gamma-amino-butanoic acid: M95 (≠ Q103), Y154 (= Y162), R157 (= R165), E231 (= E235), K288 (= K292), G316 (= G320)
- binding pyridoxal-5'-phosphate: G127 (= G135), A128 (= A136), Y154 (= Y162), H155 (= H163), D259 (= D263), V261 (≠ I265)
6j2vA Gaba aminotransferase from corynebacterium glutamicum (see paper)
44% identity, 93% coverage: 18:447/460 of query aligns to 7:435/440 of 6j2vA
- active site: L35 (≠ V43), Y154 (= Y162), D256 (= D263), K285 (= K292)
- binding 4-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]butanoic acid: G127 (= G135), A128 (= A136), Y154 (= Y162), H155 (= H163), R157 (= R165), E223 (= E230), E228 (= E235), D256 (= D263), I258 (= I265), K285 (= K292), G313 (= G320), T314 (= T321)
O58478 Alanine/serine racemase; ASR; Ala/Ser racemase; EC 5.1.1.-; EC 5.1.1.1 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see paper)
34% identity, 93% coverage: 31:457/460 of query aligns to 35:468/474 of O58478
- D251 (≠ E235) mutation to A: Loss of activity.
- K308 (= K292) mutation to A: Loss of activity.
7vo1A Structure of aminotransferase-substrate complex (see paper)
39% identity, 86% coverage: 53:447/460 of query aligns to 40:440/452 of 7vo1A
- binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-glutamic acid: I61 (= I74), S121 (≠ T134), G122 (= G135), T123 (≠ A136), F149 (≠ Y162), H150 (= H163), R152 (= R165), E234 (= E235), D262 (= D263), V264 (≠ I265), Q265 (= Q266), K291 (= K292), N318 (≠ G320), T319 (= T321), R417 (= R424)
7vntA Structure of aminotransferase-substrate complex (see paper)
39% identity, 86% coverage: 53:447/460 of query aligns to 40:440/452 of 7vntA