SitesBLAST
Comparing WP_085640004.1 NCBI__GCF_002115805.1:WP_085640004.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
6iunB Crystal structure of enoyl-coa hydratase (ech) from ralstonia eutropha h16 in complex with NAD
44% identity, 98% coverage: 6:687/695 of query aligns to 3:691/692 of 6iunB
- active site: A60 (= A62), F65 (= F67), E73 (= E73), H77 (≠ P77), G101 (= G101), E104 (= E104), E124 (= E124), G132 (= G132), K248 (≠ T246), S407 (= S405), H428 (= H426), E440 (= E438), N478 (= N476)
- binding nicotinamide-adenine-dinucleotide: G300 (= G297), T301 (= T298), M302 (= M299), E321 (= E318), T322 (≠ M319), Y365 (= Y363), A377 (= A375), V378 (= V376), E380 (= E378), V384 (= V382), V388 (= V386), N405 (= N403), S407 (= S405)
Q08426 Peroxisomal bifunctional enzyme; PBE; PBFE; L-bifunctional protein; LBP; Multifunctional enzyme 1; MFE1; EC 4.2.1.17; EC 5.3.3.8; EC 1.1.1.35 from Homo sapiens (Human) (see 5 papers)
42% identity, 97% coverage: 13:683/695 of query aligns to 11:705/723 of Q08426
- V40 (≠ E42) to G: in dbSNP:rs1062551
- I41 (≠ A43) to R: in dbSNP:rs1062552
- T75 (≠ W75) to I: in dbSNP:rs1062553
- K165 (≠ D165) modified: N6-acetyllysine; alternate; mutation to Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-171; Q-346 and Q-584.
- K171 (≠ R171) modified: N6-acetyllysine; mutation to Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-165; Q-346 and Q-584.
- A274 (= A270) to T: in dbSNP:rs2302819
- A325 (≠ L316) to G: in dbSNP:rs1062555
- K346 (= K340) modified: N6-acetyllysine; mutation to Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-165; Q-171 and Q-584.
- K584 (≠ E567) modified: N6-acetyllysine; alternate; mutation to Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-165; Q-171 and Q-346.
- K598 (≠ G578) to T: in dbSNP:rs1042437
- T606 (= T586) to P: in dbSNP:rs1042438
Sites not aligning to the query:
- 3 E → K: in FRTS3; the mutant is mistargeted to mitochondria; results in impaired mitochondrial oxidative phosphorylation and defects in the transport of fluids across the epithelium of renal proximal tubular cells; dbSNP:rs398124646
3zw9A Crystal structure of rat peroxisomal multifunctional enzyme type 1 (rpmfe1) complexed with (2s,3s)-3-hydroxy-2-methylbutanoyl-coa (see paper)
41% identity, 97% coverage: 12:683/695 of query aligns to 13:707/723 of 3zw9A
- active site: A64 (= A62), F69 (= F67), G79 (≠ P77), G103 (= G101), E106 (= E104), P125 (= P123), E126 (= E124), P133 (= P131), G134 (= G132), K252 (≠ T246), S413 (= S405), H434 (= H426), E446 (= E438), N484 (= N476)
- binding nicotinamide-adenine-dinucleotide: L305 (≠ I294), G306 (= G295), G308 (= G297), T309 (= T298), M310 (= M299), E329 (= E318), Q334 (≠ A323), A383 (= A375), V384 (= V376), F385 (= F377), E386 (= E378), N411 (= N403), S413 (= S405), H434 (= H426)
- binding (2s,3s)-3-hydroxy-2-methylbutanoyl-coa: V24 (= V23), A62 (= A60), G63 (= G61), A64 (= A62), I66 (= I64), G102 (= G100), G103 (= G101), E106 (= E104), E126 (= E124), P133 (= P131), Y159 (≠ Q157)
5omoA Crystal structure of rat peroxisomal multifunctional enzyme type-1 (rpmfe1) complexed with with 3s-hydroxy-decanoyl-coa and 3-keto- decanoyl-coa
41% identity, 97% coverage: 12:683/695 of query aligns to 15:709/725 of 5omoA
- active site: A66 (= A62), F71 (= F67), G81 (≠ P77), G105 (= G101), E108 (= E104), P127 (= P123), E128 (= E124), P135 (= P131), G136 (= G132), K254 (≠ T246), S415 (= S405), H436 (= H426), E448 (= E438), N486 (= N476)
- binding (s)-3-hydroxydecanoyl-coa: P25 (= P22), V26 (= V23), A28 (= A25), P31 (≠ Q28), A64 (= A60), A66 (= A62), D67 (= D63), I68 (= I64), L103 (= L99), G105 (= G101), E108 (= E104), P127 (= P123), E128 (= E124), Y161 (≠ Q157), F260 (= F252), K280 (≠ R272)
- binding 3-keto-decanoyl-coa: S415 (= S405), N486 (= N476), K519 (≠ A509), M520 (= M510), V525 (= V515), Y658 (= Y634)
5mgbA Crystal structure of rat peroxisomal multifunctional enzyme type-1 (rpmfe1) complexed with acetoacetyl-coa and NAD (see paper)
41% identity, 97% coverage: 12:683/695 of query aligns to 15:709/725 of 5mgbA
- active site: A66 (= A62), F71 (= F67), G81 (≠ P77), G105 (= G101), E108 (= E104), P127 (= P123), E128 (= E124), P135 (= P131), G136 (= G132), K254 (≠ T246), S415 (= S405), H436 (= H426), E448 (= E438), N486 (= N476)
- binding acetoacetyl-coenzyme a: P25 (= P22), V26 (= V23), A64 (= A60), G65 (= G61), A66 (= A62), D67 (= D63), I68 (= I64), G105 (= G101), E128 (= E124), Y161 (≠ Q157)
- binding nicotinamide-adenine-dinucleotide: L307 (≠ I294), G308 (= G295), G310 (= G297), T311 (= T298), M312 (= M299), E331 (= E318), S332 (≠ M319), Q336 (≠ A323), V386 (= V376), F387 (= F377), E388 (= E378), N413 (= N403), S415 (= S405), H436 (= H426)
3zwcA Crystal structure of rat peroxisomal multifunctional enzyme type 1 (rpmfe1) complexed with 3s-hydroxy-decanoyl-coa (see paper)
41% identity, 97% coverage: 12:683/695 of query aligns to 15:709/725 of 3zwcA
- active site: A66 (= A62), F71 (= F67), G81 (≠ P77), G105 (= G101), E108 (= E104), P127 (= P123), E128 (= E124), P135 (= P131), G136 (= G132), K254 (≠ T246), S415 (= S405), H436 (= H426), E448 (= E438), N486 (= N476)
- binding (s)-3-hydroxydecanoyl-coa: V26 (= V23), A64 (= A60), G65 (= G61), A66 (= A62), D67 (= D63), I68 (= I64), G77 (≠ E73), L78 (≠ P74), L80 (= L76), V101 (≠ T97), G104 (= G100), G105 (= G101), E108 (= E104), E128 (= E124), F260 (= F252)
- binding nicotinamide-adenine-dinucleotide: G308 (= G295), G310 (= G297), T311 (= T298), M312 (= M299), E331 (= E318), Q336 (≠ A323), A385 (= A375), V386 (= V376), F387 (= F377), E388 (= E378), K393 (= K383), N413 (= N403), S415 (= S405), H436 (= H426)
2x58A The crystal structure of mfe1 liganded with coa (see paper)
41% identity, 97% coverage: 12:683/695 of query aligns to 15:709/725 of 2x58A