SitesBLAST

T97 (≠ L85) binding pyridoxal 5'-phosphate
S98 (= S86) binding pyridoxal 5'-phosphate
D132 (= D116) mutation to A: Reduces binding to pyridoxal phosphate and strongly reduces catalytic activity.; mutation to E: Enhances binding to pyridoxal phosphate.
T172 (= T155) binding pyridoxal 5'-phosphate
D201 (= D184) binding pyridoxal 5'-phosphate; mutation to E: Enhances binding to pyridoxal phosphate.
H204 (= H187) binding pyridoxal 5'-phosphate

1qz9A The three dimensional structure of kynureninase from pseudomonas fluorescens (see paper)
52% identity, 95% coverage: 8:385/397 of query aligns to 19:403/404 of 1qz9A

query
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active site: F128 (= F113), D200 (= D184), Y225 (= Y209), K226 (= K210), R374 (= R356)
binding pyridoxal-5'-phosphate: T95 (= T84), T96 (≠ L85), S97 (= S86), F128 (= F113), D131 (= D116), T171 (= T155), D200 (= D184), A202 (= A186), H203 (= H187), C223 (= C207), Y225 (= Y209), K226 (= K210)

P70712 Kynureninase; L-kynurenine hydrolase; EC 3.7.1.3 from Rattus norvegicus (Rat) (see paper)
29% identity, 91% coverage: 18:380/397 of query aligns to 69:458/464 of P70712

query
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P70712

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Sites not aligning to the query:

1 modified: N-acetylmethionine

3e9kA Crystal structure of homo sapiens kynureninase-3-hydroxyhippuric acid inhibitor complex (see paper)
28% identity, 92% coverage: 15:380/397 of query aligns to 61:444/446 of 3e9kA

query
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active site: F160 (= F113), D245 (= D184), Y270 (= Y209), K271 (= K210), R420 (= R356)
binding 3-Hydroxyhippuric acid: S70 (= S24), F160 (= F113), H248 (= H187), K271 (= K210), R420 (= R356)
binding pyridoxal-5'-phosphate: L132 (= L85), T133 (≠ S86), F160 (= F113), D163 (= D116), D245 (= D184), A247 (= A186), H248 (= H187), Y270 (= Y209), K271 (= K210)

Q16719 Kynureninase; L-kynurenine hydrolase; EC 3.7.1.3 from Homo sapiens (Human) (see 4 papers)
28% identity, 92% coverage: 15:380/397 of query aligns to 66:458/465 of Q16719

query
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Q16719

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K

N

V

L

F

H

Q

L

A

L

L

M

A

L

A

S

A

F

L

F

K

K

L

P

N

T

P

P

H

K

R

R

R
x
Y

V
x
K

I
|
I

L
|
L

S
x
L

D
x
E

T
x
A

G
x
K

N
x
A

F
|
F

P
|
P

S
|
S

D
|
D

L
x
H

Y
|
Y

M
x
A

A
x
I

E
|
E

G
x
S

L
x
Q

I
x
L

S
x
Q

L
|
L

L
x
H

G
|
G

P
x
L

D
x
N

Y
x
I

E
|
E

-
x
E

-
x
S

L
x
M

R
|
R

L
x
M

V
x
I

A
x
K

P
|
P

E
x
R

E
|
E

V
x
G

G
x
E

A
x
E

A
x
T

I
x
L

T
x
R

-
x
I

-
x
E

-
x
D

-
x
I

-
x
L

-
x
E

-
x
V

-
x
I

-
x
E

-
x
K

-
x
E

-
x
G

D
|
D

E
x
S

I
|
I

A
|
A

V
|
V

T
x
I

L
|
L

I
x
F

T
x
S

E
x
G

V
|
V

D
x
H

Y
x
F

R
x
Y

T
|
T

G
|
G

R
x
Q

K
x
H

H
x
F

D
x
N

M
x
I

A
x
P

A
|
A

L
x
I

T
|
T

A
x
K

K
x
A

A
x
G

H
x
Q

A
|
A

N
x
K

G
|
G

V
x
C

V
x
Y

T
x
V

V
x
G

W
x
F

D
|
D

L
|
L

A
|
A

H
|
H

S
x
A

A
x
V

G
|
G

A
x
N

I
x
V

P
x
E

V
x
L

D
x
Y

L
|
L

A
x
H

G
x
D

A
x
W

K
x
G

A
x
V

D
|
D

F
|
F

A
|
A

I
x
C

G
x
W

C
|
C

T
x
S

Y
|
Y

K
|
K

Y
|
Y

L
|
L

N
|
N

G
x
A

G
|
G

P
x
A

G
|
G

A
x
G

P
x
I

A
|
A

F
x
G

I
x
A

Y
x
F

V
x
I

A
x
H

P
x
E

R
x
K

H
|
H

A
|
A

D
x
H

M
x
T

A
x
I

L
x
K

P
|
P

A
|
A

L
|
L

S
x
V

G
|
G

W
|
W

L
x
F

G
|
G

H
|
H

D
x
E

S
x
L

P
x
S

F
x
T

A
x
R

F
|
F

D
x
K

L
x
M

G
x
D

Y
x
N

K
|
K

A
x
L

G
x
Q

V
x
L

-
x
I

-
x
P

G
|
G

I
x
V

S
x
C

R
x
G

M
x
F

R
|
R

V
x
I

G
x
S

T
x
N

P
|
P

V
x
I

L
|
L

Q
x
L

M
x
V

A
x
C

A
x
S

L
|
L

D
x
H

A
|
A

A
x
S

L
|
L

D
x
E

V
x
I

W
x
F

D
x
K

D
x
Q

V
x
A

D
x
T

L
x
M

N
x
K

D
x
A

L
|
L

R
|
R

T
x
K

R
x
K

S
|
S

I
x
V

D
x
L

L
|
L

S
x
T

-
x
G

-
x
Y

-
x
L

E
|
E

R
x
Y

F
x
L

I
|
I

A
x
K

-
x
H

-
x
N

-
x
Y

-
x
G

-
x
K

-
x
D

G
x
K

V
x
A

E
x
A

A
x
T

T
x
K

C
x
K

P
|
P

D
x
V

L
x
V

T
x
N

L
x
I

A
x
I

S
x
T

P
|
P

R
x
S

N
x
H

A
x
V

A
x
E

E
|
E

R
|
R

G
|
G

S
x
C

Q
|
Q

V
x
L

S
x
T

F
x
I

K
x
T

F
|
F

E
x
S

-
x
V

E
x
P

G
x
N

Y
x
K

A
x
D

A
x
V

M
x
F

Q
|
Q

A
x
E

L
|
L

I
x
E

A
x
K

H
x
R

K
x
G

V
|
V

I
x
V

G
x
C

D
|
D

F
x
K

R
|
R

A
x
N

P
|
P

D
x
N

I
x
G

M
x
I

R
|
R

F
x
V

G
x
A

F
x
P

T
x
V

P
|
P

L
|
L

Y
|
Y

L
x
N

I
x
S

L
x
F

E
x
H

D
|
D

V
|
V

D
x
Y

Q
x
K

A
x
F

V
x
T

E
x
N

R
x
L

L
|
L

A
x
T

R
x
S

I
|
I

M
x
L

T138 (≠ S86) binding pyridoxal 5'-phosphate
T198 (≠ A144) to A: in HYXKY; reduced 3-hydroxykynureninase activity; dbSNP:rs606231307
D250 (= D184) binding pyridoxal 5'-phosphate
H253 (= H187) binding pyridoxal 5'-phosphate
Y275 (= Y209) binding pyridoxal 5'-phosphate
W305 (= W239) binding pyridoxal 5'-phosphate
N333 (≠ T265) binding pyridoxal 5'-phosphate
K412 (≠ Y334) to E: in dbSNP:rs9013

Sites not aligning to the query:

156:465 natural variant: Missing (in VCRL2; strongly reduced 3-hydroxykynureninase activity)

2hzpA Crystal structure of homo sapiens kynureninase (see paper)
28% identity, 92% coverage: 15:380/397 of query aligns to 61:445/447 of 2hzpA

query
sites
2hzpA

E

E

G

N

M

A

I

I

Y

Y

L

F

D

L

G

G

N

N

S

S

L

L

G

G

P

L

L

Q

P

P

K

K

S

M

A

V

A

K

A

T

R

Y

V

L

A

E

Q

E

T

E

V

L

A

-

S

D

E

K

W

W

G

A

D

K

H

I

L

A

I

A

T

Y

G

G

W

H

N

E

A

V

C

G

G

K

-

R

-

P

W

W

M

I

D

T

M

G

P

D

A

E

R

S

L

I

G

V

D

G

Q

L

I

M

G

K

R

D

L

I

I

V

G

G

A

A

E

N

A

E

G

K

H

E

V

I

V

A

V

L

G

M

D

N

T

A

L
|
L

S
x
T

I

V

K

N

V

L

F

H

Q

L

A

L

L

M

A

L

A

S

A

F

L

F

K

K

L

P

N

T

P

P

H

K

R

R

R

Y

V

K

I

I

L

L

S

L

D

E

T

A

G

K

N

A

F
|
F

P

P

S

S

D
|
D

L

H

Y

Y

M

A

A

I

E

E

G

S

L

Q

I

L

S

Q

L

L

L

H

G

G

P

L

D

N

Y

I

E

E

-

E

-

S

L

M

R

R

L

M

V

I

A

K

P

P

E

R

E

E

V

G

G

E

A

E

A

T

I

L

T

R

-

I

-

E

-

D

-

I

-

L

-

E

-

V

-

I

-

E

-

K

-

E

-

G

D

D

E

S

I

I

A

A

V

V

T

I

L

L

I

F

T

S

E

G

V

V

D

H

Y

F

R

Y

T

T

G

G

R

Q

K

H

H

F

D

N

M

I

A

P

A

A

L

I

T

T

A

K

K

A

A

G

H

Q

A

A

N

K

G

G

V

C

V

Y

T

V

V

G

W

F

D
|
D

L

L

A
|
A

H
|
H

S

A

A

V

G

G

A

N

I

V

P

E

V

L

D

Y

L

L

A

H

G

D

A

W

K

G

A

V

D

D

F

F

A

A

I

C

G

W

C

C

T

S

Y
|
Y

K
|
K

Y

Y

L

L

N

N

G

A

G

G

P

A

G

G

A

G

P

I

A

A

F

G

I

A

Y

F

V

I

A

H

P

E

R

K

H

H

A

A

D

H

M

T

A

I

L

K

P

P

A

A

L

L

S

V

G

G

W

W

L

F

G

G

H

H

D

E

S

L

P

S

F

T

A

R

F

F

D

K

L

M

G

D

Y

N

K

K

A

L

G

Q

V

L

-

I

-

P

G

G

I

V

S

C

R

G

M

F

R

R

V

I

G

S

T

N

P

P

V

I

L

L

Q

L

M

V

A

C

A

S

L

L

D

H

A

A

A

S

L

L

D

E

V

I

W

F

D

K

D

Q

V

A

D

T

L

M

N

K

D

A

L

L

R

R

T

K

R

K

S

S

I

V

D

L

L

L

S

T

-

G

-

Y

-

L

E

E

R

Y

F

L

I

I

A

K

G

H

V

N

E

Y

A

G

T

K

C

V

P

-

D

-

L

V

T

N

L

I

A

I

S

T

P

P

R

S

N

H

A

V

A

E

E

E

R

R

G

G

S

C

Q

Q

V

L

S

T

F

I

K

T

F

F

E

S

-

V

E

P

G

N

Y

K

A

D

A

V

M

F

Q

Q

A

E

L

L

I

E

A

K

H

R

K

G

V

V

I

V

G

C

D

D

F

K

R

R

A

N

P

P

D

N

I

G

M

I

R
|
R

F

V

G

A

F

P

T

V

P

P

L

L

Y

Y

L

N

I

S

L

F

E

H

D

D

V

V

D

Y

Q

K

A

F

V

T

E

N

R

L

L

L

A

T

R

S

I

I

M

L

active site: F160 (= F113), D245 (= D184), Y270 (= Y209), K271 (= K210), R421 (= R356)
binding pyridoxal-5'-phosphate: L132 (= L85), T133 (≠ S86), F160 (= F113), D163 (= D116), D245 (= D184), A247 (= A186), H248 (= H187), Y270 (= Y209), K271 (= K210)

3lvmB Crystal structure of e.Coli iscs (see paper)
25% identity, 83% coverage: 10:340/397 of query aligns to 6:323/394 of 3lvmB

query
sites
3lvmB

A

A

F

M

H

K

L

L

P

P

E

-

G

-

M

-

I

I

Y

Y

L

L

D

D

G

Y

N

S

S

A

L

T

G

T

P

P

L

V

P

D

K

P

S

R

A

V

A

A

A

E

R

K

V

M

A

M

Q

Q

-

F

-

M

T

T

V

M

A

D

S

G

E

T

W

F

G

G

D

N

-

P

-

A

-

S

-

R

-

S

H

H

L

R

I

F

T

-

G

G

W

W

N

Q

A

A

C

E

G

E

W

A

M

V

D

D

M

I

P

-

A

A

R

R

L

-

G

-

D

N

Q

Q

I

I

G

A

R

D

L

L

I

V

G

G

A

A

E

D

A

P

G

R

H

E

V

I

V

V

V

F

-

T

-

S

-
x
G

-
x
A

-
x
T

-

E

G

S

D

D

T

N

L

L

S

A

I

I

K

K

V

G

F

-

Q

-

A

-

L

-

A

A

A

N

L

F

K

Y

L

Q

N

K

P

K

H

G

R

K

R

H

V

I

I

I

L

T

S

S

D

K

T

T

G

E

N
x
H

F

K

P

A

S

-

D

-

L

-

Y

-

M

V

A

L

E

D

G

T

L

C

I

R

S

Q

L

L

L

E

G

R

P

E

D

G

Y

F

E

E

L

V

R

T

L

Y

V

L

A

A

P

P

E

Q

-

R

-

N

-

G

-

I

-

D

-

L

-

K

E

E

V

L

G

E

A

A

I

M

T

R

D

D

E

D

I

T

A

I

V

L

T

V

L

S

I

I

T

M

E

H

V

V

D

N

Y

N

R

E

T

I

G

G

R

V

K

V

H

Q

D

D

M

I

A

A

L

I

T

G

A

E

K

M

A

C

H

R

A

A

N

R

G

G

V

I

T

Y

V

H

W

V

D
|
D

L

A

A
x
T

H
x
Q

S

S

A

V

G

G

A

K

I

L

P

P

V

I

D

D

L

L

A

S

G

Q

A

L

K

K

A

V

D

D

F

L

A

M

I

S

G

F

C

S

T

G

Y
x
H

K
|
K

Y

I

L

Y

N

-

G

-

G

G

P

P

G

K

A

G

P

I

A

G

F

A

I

L

Y

Y

V

V

A

R

P

-

R

R

H

K

A

P

D

R

M

V

A

R

L

I

P

E

A

A

L

Q

S

M

G

H

W

G

L

G

G

G

H

H

D

E

S

R

P

G

F

-

A

-

F

-

D

-

L

-

G

-

Y

-

K

-

A

-

G

-

V

-

G

-

I

-

S

-

R

-

M

M

R

R

V

S

G

G

T

T

P

L

P

P

V

V

L

H

Q

Q

M

I

A

V

A

G

L

M

D

G

A

E

A

A

L

Y

D

R

V

I

W

A

D

K

D

E

-

E

-

M

-

A

-

T

-

E

-

M

V

E

D

R

L

L

N

R

D

G

L

L

R

R

T

N

R

R

-

L

-

W

-

N

-

G

S

I

I

K

D

D

L

I

S

E

E

E

R

V

F

Y

I

L

A

N

G

G

-

D

V

L

E

E

A

H

T

G

C

A

P

P

D

N

L

I

T

L

L

-

A

-

S

-

P

-

R

-

N

-

A

-

E

-

R

-

G

-

S

-

Q

N

V

V

S

S

F

F

K

N

F

Y

E

V

E

E

G

G

Y

E

A

S

A

L

M

I

Q

M

A

A

L

L

active site: H110 (≠ N112), D186 (= D184), T188 (≠ A186), Q189 (≠ H187), K212 (= K210)
binding pyridoxal-5'-phosphate: G80 (vs. gap), A81 (vs. gap), T82 (vs. gap), H110 (≠ N112), D186 (= D184), T188 (≠ A186), Q189 (≠ H187), H211 (≠ Y209), K212 (= K210)

Sites not aligning to the query:

active site: 329

P0A6B7 Cysteine desulfurase IscS; NifS protein homolog; ThiI transpersulfidase; TusA transpersulfidase; EC 2.8.1.7 from Escherichia coli (strain K12) (see 4 papers)
25% identity, 81% coverage: 18:340/397 of query aligns to 5:317/404 of P0A6B7

query
sites
P0A6B7

I

I

Y

Y

L

L

D

D

G

Y

N

S

S

A

L

T

G

T

P

P

L

V

P

D

K

P

S

R

A

V

A

A

A

E

R

K

V

M

A

M

Q

Q

-

F

-

M

T

T

V

M

A

D

S

G

E

T

W

F

G

G

D

N

-

P

-

A

-

S

-

R

-

S

H

H

L

R

I

F

T

-

G

G

W

W

N

Q

A

A

C

E

G

E

W

A

M

V

D

D

M

I

P

-

A

A

R

R

L

-

G

-

D

N

Q

Q

I

I

G

A

R

D

L

L

I

V

G

G

A

A

E

D

A

P

G

R

H

E

V

I

V

V

V

F

-

T

-

S

-

G

-

A

-

T

-

E

G

S

D

D

T

N

L

L

S

A

I

I

K

K

V

G

F

-

Q

-

A

-

L

-

A

A

A

N

L

F

K

Y

L

Q

N

K

P

K

H

G

R

K

R

H

V

I

I

I

L

T

S

S

D

K

T

T

G

E

N

H

F

K

P

A

S

-

D

-

L

-

Y

-

M

V

A

L

E

D

G

T

L

C

I

R

S

Q

L

L

L

E

G

R

P

E

D

G

Y

F

E

E

L

V

R

T

L

Y

V

L

A

A

P

P

E

Q

-

R

-

N

-

G

-

I

-

D

-

L

-

K

E

E

V

L

G

E

A

A

I

M

T

R

D

D

E

D

I

T

A

I

V

L

T

V

L

S

I

I

T

M

E

H

V

V

D

N

Y

N

R

E

T

I

G

G

R

V

K

V

H

Q

D

D

M

I

A

A

L

I

T

G

A

E

K

M

A

C

H

R

A

A

N

R

G

G

V

I

T

Y

V

H

W

V

D

D

L

A

A

T

H
x
Q

S

S

A

V

G

G

A

K

I

L

P

P

V

I

D

D

L

L

A

S

G

Q

A

L

K

K

A

V

D

D

F

L

A

M

I

S

G

F

C
x
S

T
x
G

Y
x
H

K
|
K

Y

I

L

Y

N

-

G

-

G

G

P

P

G

K

A

G

P

I

A

G

F

A

I

L

Y

Y

V

V

A

R

P

-

R

R

H

K

A

P

D

R

M

V

A

R

L

I

P

E

A

A

L

Q

S

M

G

H

W

G

L

G

G

G

H

H

D

E

S

R

P

G

F

-

A

-

F

-

D

-

L

-

G

-

Y

-

K

-

A

-

G

-

V

-

G

-

I

-

S

-

R

-

M

M

R

R

V

S

G

G

T
|
T

P

L

P

P

V

V

L

H

Q

Q

M

I

A

V

A

G

L

M

D

G

A

E

A

A

L

Y

D

R

V

I

W

A

D

K

D

E

-

E

-

M

-

A

-

T

-

E

-

M

V

E

D

R

L

L

N

R

D

G

L

L

R

R

T

N

R

R

-

L

-

W

-

N

-

G

S

I

I

K

D

D

L

I

S

E

E

E

R

V

F

Y

I

L

A

N

G

G

-

D

V

L

E

E

A

H

T

G

C

A

P

P

D

N

L

I

T

L

L

-

A

-

S

-

P

-

R

-

N

-

A

-

E

-

R

-

G

-

S

-

Q

N

V

V

S

S

F

F

K

N

F

Y

E

V

E

E

G

G

Y

E

A

S

A

L

M

I

Q

M

A

A

L

L

Q183 (≠ H187) binding pyridoxal 5'-phosphate
SGH 203:205 (≠ CTY 207:209) binding pyridoxal 5'-phosphate
K206 (= K210) modified: N6-(pyridoxal phosphate)lysine
T243 (= T265) binding pyridoxal 5'-phosphate

Sites not aligning to the query:

328 active site, Cysteine persulfide intermediate; C→A: Loss of cysteine desulfurization.
376:404 mutation Missing: Normal cysteine desulfurase activity, decreased binding to IscU, decreased sulfur transfer to IscU, decreased Fe-S cluster assembly.

P0A6B9 Cysteine desulfurase IscS; EC 2.8.1.7 from Escherichia coli O157:H7 (see paper)
25% identity, 81% coverage: 18:340/397 of query aligns to 5:317/404 of P0A6B9

query
sites
P0A6B9

I

I

Y

Y

L

L

D

D

G

Y

N

S

S

A

L

T

G

T

P

P

L

V

P

D

K

P

S

R

A

V

A

A

A

E

R

K

V

M

A

M

Q

Q

-

F

-

M

T

T

V

M

A

D

S

G

E

T

W

F

G

G

D

N

-

P

-

A

-

S

-
x
R

-

S

H

H

L

R

I

F

T

-

G

G

W
|
W

N

Q

A

A

C

E

G
x
E

W

A

M

V

D
|
D

M

I

P

-

A

A

R

R

L

-

G

-

D

N

Q

Q

I

I

G

A

R

D

L

L

I

V

G

G

A

A

E
x
D

A

P

G

R

H

E

V

I

V

V

V

F

-

T

-

S

-

G

-
x
A

-
x
T

-

E

G

S

D

D

T

N

L

L

S

A

I

I

K

K

V

G

F

-

Q

-

A

-

L

-

A

A

A

N

L
x
F

K

Y

L

Q

N

K

P

K

H

G

R

K

R

H

V

I

I

I

L

T

S

S

D

K

T

T

G

E

N

H

F

K

P

A

S

-

D

-

L

-

Y

-

M

V

A

L

E

D

G

T

L

C

I
x
R

S

Q

L

L

L

E

G
x
R

P

E

D

G

Y

F

E

E

L

V

R

T

L

Y

V

L

A

A

P

P

E

Q

-

R

-

N

-

G

-

I

-

D

-

L

-

K

E

E

V

L

G

E

A

A

I

M

T

R

D

D

E

D

I

T

A

I

V

L

T

V

L

S

I

I

T

M

E

H

V

V

D

N

Y

N

R

E

T

I

G

G

R

V

K

V

H

Q

D

D

M

I

A

A

L

I

T

G

A

E

K

M

A

C

H

R

A

A

N

R

G

G

V

I

T

Y

V

H

W

V

D

D

L

A

A

T

H
x
Q

S

S

A

V

G

G

A

K

I

L

P

P

V

I

D

D

L

L

A

S

G

Q

A

L

K

K

A

V

D

D

F

L

A

M

I

S

G

F

C
x
S

T
x
G

Y
x
H

K
|
K

Y

I

L

Y

N

-

G

-

G

G

P

P

G

K

A

G

P

I

A

G

F

A

I

L

Y

Y

V

V

A

R

P

-

R
|
R

H

K

A

P

D
x
R

M
x
V

A
x
R

L
x
I

P
x
E

A

A

L

Q

S

M

G

H

W

G

L

G

G
|
G

H

H

D

E

S
x
R

P
x
G

F

-

A

-

F

-

D

-

L

-

G

-

Y

-

K

-

A

-

G

-

V

-

G

-

I

-

S

-

R

-

M
|
M

R

R

V

S

G

G

T
|
T

P

L

P

P

V

V

L

H

Q

Q

M

I

A

V

A

G

L

M

D

G

A

E

A

A

L

Y

D

R

V

I

W

A

D

K

D

E

-

E

-

M

-

A

-

T

-

E

-

M

V

E

D

R

L

L

N

R

D

G

L

L

R

R

T

N

R

R

-

L

-

W

-

N

-

G

S

I

I

K

D

D

L

I

S

E

E

E

R

V

F

Y

I

L

A

N

G

G

-

D

V

L

E

E

A

H

T

G

C

A

P

P

D

N

L

I

T

L

L

-

A

-

S

-

P

-

R

-

N

-

A

-

E

-

R

-

G

-

S

-

Q

N

V

V

S

S

F

F

K

N

F

Y

E

V

E

E

G

G

Y
x
E

A

S

A

L

M

I

Q

M

A

A

L

L

R39 (vs. gap) mutation to E: Decreased binding to CyaY.
W45 (= W52) mutation to R: No binding to TusA, decreased binding to ThiI. 3% 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U), 7% 4-thiouridine produced.
E49 (≠ G56) mutation to A: No binding to TusA. 24% mnm(5)s(2)U tRNA produced.
D52 (= D59) mutation D->A,M,R,Y: No binding to TusA. 0-20% mnm(5)s(2)U tRNA produced.
D65 (≠ E75) mutation to F: Decreased binding to TusA. 22% mnm(5)s(2)U tRNA produced.
AT 75:76 (vs. gap) binding pyridoxal 5'-phosphate
F89 (≠ L97) mutation to E: Decreased binding to ThiI.
R112 (≠ I124) mutation to E: Decreased binding to IscX.
R116 (≠ G128) mutation to E: Decreased binding to CyaY, IscX, ThiI.
Q183 (≠ H187) binding pyridoxal 5'-phosphate
SGH 203:205 (≠ CTY 207:209) binding pyridoxal 5'-phosphate
K206 (= K210) modified: N6-(pyridoxal phosphate)lysine
R220 (= R227) mutation to E: No binding to CyaY, IscX, ThiI.
R223 (≠ D230) mutation to E: No binding CyaY, IscX, decreased binding to ThiI.
RVR 223:225 (≠ DMA 230:232) mutation to EVE: No binding to IscX.
RIE 225:227 (≠ ALP 232:234) mutation to EIR: No binding to CyaY, IscX.
G234 (= G241) mutation to L: Decreased binding to CyaY, IscX.
RG---------------M 237:239 (≠ SPFAFDLGYKAGVGISRM 244:261) mutation to EGE: No binding to CyaY, IscX, ThiI.
T243 (= T265) binding pyridoxal 5'-phosphate
E311 (≠ Y334) mutation to R: Decreased binding to ThiI.

Sites not aligning to the query:

327 A→V: No binding to IscX, decreased binding to CyaY, IscU, ThiI.
328 C→S: Retains binding to IscU, ThiI.
340 R→E: No binding to CyaY, ThiI, decreased binding to IscX, TusA.

6o11A E. Coli cysteine desulfurase sufs c364a with a cys-aldimine intermediate (see paper)
25% identity, 64% coverage: 127:381/397 of query aligns to 146:403/406 of 6o11A

query
sites
6o11A

L

L

G

N

P

P

D

D

Y

G

E

T

L

L

R

Q

L

L

V

-

A

-

P

-

E

E

E

T

V

L

G

P

A

T

A

L

I

F

T

D

D

E

E

K

I

T

A

R

V

L

T

L

L

A

I

I

T

T

E

H

V

V

D

S

Y
x
N

R

V

T

L

G

G

R

T

K

E

H

N

D

P

M

L

A

A

A

E

L

M

T

I

A

T

K

L

A

A

H

H

A

Q

N

H

G

G

V

A

V

K

T

V

V

L

W

V

D
|
D

L

G

A
|
A

H
x
Q

S

A

A

V

G

M

A

H

I

H

P

P

V

V

D

D

L

V

A

Q

G

A

A

L

K

D

A

C

D

D

F

F

A

Y

I

V

G

F

C
x
S

T

G

Y
x
H

K
|
K

Y

L

L

Y

N

-

G

-

G

G

P

P

G

T

A

G

P

I

A

G

F

I

I

L

Y

Y

V

V

A

-

P

-

R

-

H

-

A

K

D

E

M

A

A

L

L

L

P

Q

A

E

L

M

S

P

G

P

W

W

L

E

G

G

H

G

D

G

S

S

P

M

F

I

A

A

-

T

-

V

-

S

F

L

D

S

L

E

G

G

Y

T

K

T

A

W

G

T

V

K

G

A

I

P

S

W

R

R

M

F

R

E

V

A

G

G

T

T

P

P

P

N

V

T

L

G

Q

G

M

I

A

I

A

G

L

L

D

G

A

A

L

L

D

E

V

Y

W

V

D

S

D

A

V

L

D

G

L

L

N

N

D

N

L

I

R

A

T

E

R

Y

S

E

I

Q

D

N

L

L

S

M

E

H

R

Y

F

A

I

L

A

S

G

Q

V

L

E

E

A

-

T

S

C

V

P

P

D

D

L

L

T

T

L

L

A

Y

S

G

P

P

R

Q

N

N

-

R

-

L

-

G

-

V

-

I

-

A

-

F

-

N

-

L

-

G

-

K

-

H

A

A

A

Y

E

D

R

V

G

G

S

S

-

F

-

L

-

D

Q

N

V

Y

S

G

F

I

K

A

F

V

E
x
R

E

T

G

G

Y

H

A

H

A

A

M

A

Q

M

A

P

L

L

I

M

A

A

H

Y

K

-

V

-

I

-

G

-

D

-

F

Y

R

N

A

V

P

P

D

A

I

M

M

C

R
|
R

F

A

G

S

F

L

T

A

P

-

L

M

Y

Y

L

N

I

T

L

H

E

E

D

E

V

V

D

D

Q

R

A

L

V

V

E

T

R

G

L

L

A

Q

R

R

I

I

M

H

R

R

binding n-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-l-cysteine: N175 (≠ Y159), D200 (= D184), A202 (= A186), Q203 (≠ H187), S223 (≠ C207), H225 (≠ Y209), K226 (= K210), R359 (≠ E331), R379 (= R356)

Sites not aligning to the query:

binding n-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-l-cysteine: 30, 31, 94, 95, 123

5db5A Crystal structure of plp-bound e. Coli sufs (cysteine persulfide intermediate) in space group p21
25% identity, 64% coverage: 127:381/397 of query aligns to 144:401/404 of 5db5A

query
sites
5db5A

L

L

G

N

P

P

D

D

Y

G

E

T

L

L

R

Q

L

L

V

-

A

-

P

-

E

E

E

T

V

L

G

P

A

T

A

L

I

F

T

D

D

E

E

K

I

T

A

R

V

L

T

L

L

A

I

I

T

T

E

H

V

V

D

S

Y

N

R

V

T

L

G

G

R

T

K

E

H

N

D

P

M

L

A

A

A

E

L

M

T

I

A

T

K

L

A

A

H

H

A

Q

N

H

G

G

V

A

V

K

T

V

V

L

W

V

D
|
D

L

G

A
|
A

H
x
Q

S

A

A

V

G

M

A

H

I

H

P

P

V

V

D

D

L

V

A

Q

G

A

A

L

K

D

A

C

D

D

F

F

A

Y

I

V

G

F

C
x
S

T

G

Y
x
H

K
|
K

Y

L

L

Y

N

-

G

-

G

G

P

P

G

T

A

G

P

I

A

G

F

I

I

L

Y

Y

V

V

A

-

P

-

R

-

H

-

A

K

D

E

M

A

A

L

L

L

P

Q

A

E

L

M

S

P

G

P

W

W

L

E

G

G

H

G

D

G

S

S

P

M

F

I

A

A

-

T

-

V

-

S

F

L

D

S

L

E

G

G

Y

T

K

T

A

W

G

T

V

K

G

A

I

P

S

W

R

R

M

F

R

E

V

A

G
|
G

T
|
T

P

P

P

N

V

T

L

G

Q

G

M

I

A

I

A

G

L

L

D

G

A

A

L

L

D

E

V

Y

W

V

D

S

D

A

V

L

D

G

L

L

N

N

D

N

L

I

R

A

T

E

R

Y

S

E

I

Q

D

N

L

L

S

M

E

H

R

Y

F

A

I

L

A

S

G

Q

V

L

E

E

A

-

T

S

C

V

P

P

D

D

L

L

T

T

L

L

A

Y

S

G

P

P

R

Q

N

N

-

R

-

L

-

G

-

V

-

I

A

A

A

F

E

N

R

L

G

G

S

K

Q

H

V

H

S

A

F

Y

-

D

-

V

-

G

K

S

F

F

E

L

E

D

G

N

Y

Y

A

G

A

I

M

A

Q

V

A
x
R

L

T

I

G

A

H

H

H

-
x
C

-

A

-

M

K

P

V

L

I

M

G

A

D

Y

F

Y

R

N

A

V

P

P

D

A

I

M

M

C

R
|
R

F

A

G

S

F

L

T

A

P

-

L

M

Y

Y

L

N

I

T

L

H

E

E

D

E

V

V

D

D

Q

R

A

L

V

V

E

T

R

G

L

L

A

Q

R

R

I

I

M

H

R

R

active site: D198 (= D184), A200 (= A186), Q201 (≠ H187), K224 (= K210), R357 (≠ A339), C362 (vs. gap)
binding cysteine: R377 (= R356)
binding pyridoxal-5'-phosphate: D198 (= D184), A200 (= A186), Q201 (≠ H187), S221 (≠ C207), H223 (≠ Y209), K224 (= K210), G275 (= G264), T276 (= T265)

Sites not aligning to the query:

active site: 121
binding cysteine: 29
binding pyridoxal-5'-phosphate: 92, 93, 121

6ndnA Crystal structure of selenocysteine lyase from escherichia coli
25% identity, 64% coverage: 127:381/397 of query aligns to 146:403/406 of 6ndnA

query
sites
6ndnA

L

L

G

N

P

P

D

D

Y

G

E

T

L

L

R

Q

L

L

V

-

A

-

P

-

E

E

E

T

V

L

G

P

A

T

A

L

I

F

T

D

D

E

E

K

I

T

A

R

V

L

T

L

L

A

I

I

T

T

E

H

V

V

D

S

Y
x
N

R

V

T

L

G

G

R

T

K

E

H

N

D

P

M

L

A

A

A

E

L

M

T

I

A

T

K

L

A

A

H

H

A

Q

N

H

G

G

V

A

V

K

T

V

V

L

W

V

D
|
D

L

G

A
|
A

H
x
Q

S

A

A

V

G

M

A

H

I

H

P

P

V

V

D

D

L

V

A

Q

G

A

A

L

K

D

A

C

D

D

F

F

A

Y

I

V

G

F

C
x
S

T

G

Y
x
H

K
|
K

Y

L

L

Y

N

-

G

-

G

G

P

P

G

T

A

G

P

I

A

G

F

I

I

L

Y

Y

V

V

A

-

P

-

R

-

H

-

A

K

D

E

M

A

A

L

L

L

P

Q

A

E

L

M

S

P

G

P

W

W

L

E

G

G

H

G

D

G

S

S

P

M

F

I

A

A

-

T

-

V

-

S

F

L

D

S

L

E

G

G

Y

T

K

T

A

W

G

T

V

K

G

A

I

P

S

W

R

R

M

F

R

E

V

A

G

G

T

T

P

P

P

N

V

T

L

G

Q

G

M

I

A

I

A

G

L

L

D

G

A

A

L

L

D

E

V

Y

W

V

D

S

D

A

V

L

D

G

L

L

N

N

D

N

L

I

R

A

T

E

R

Y

S

E

I

Q

D

N

L

L

S

M

E

H

R

Y

F

A

I

L

A

S

G

Q

V

L

E

E

A

-

T

S

C

V

P

P

D

D

L

L

T

T

L

L

A

Y

S

G

P

P

R

Q

N

N

-

R

-

L

-

G

-

V

-

I

A

A

A

F

E

N

R

L

G

G

S

K

Q

H

V

H

S

A

F

Y

-

D

-

V

-

G

K

S

F

F

E

L

E

D

G

N

Y

Y

A

G

A

I

M

A

Q

V

A

R

L

T

I

G

A

H

H

H

-

C

-

A

-

M

K

P

V

L

I

M

G

A

D

Y

F

Y

R

N

A

V

P

P

D

A

I

M

M

C

R

R

F

A

G

S

F

L

T

A

P

-

L

M

Y

Y

L

N

I

T

L

H

E

E

D

E

V

V

D

D

Q

R

A

L

V

V

E

T

R

G

L

L

A

Q

R

R

I

I

M

H

R

R

binding pyridoxal-5'-phosphate: N175 (≠ Y159), D200 (= D184), A202 (= A186), Q203 (≠ H187), S223 (≠ C207), H225 (≠ Y209), K226 (= K210)

Sites not aligning to the query:

binding pyridoxal-5'-phosphate: 94, 95, 123

P77444 Cysteine desulfurase; Cysteine sulfinate desulfinase; CSD; Selenocysteine beta-lyase; SCL; Selenocysteine lyase; Selenocysteine reductase; EC 2.8.1.7; EC 3.13.1.-; EC 4.4.1.16 from Escherichia coli (strain K12) (see 2 papers)
25% identity, 64% coverage: 127:381/397 of query aligns to 146:403/406 of P77444

query
sites
P77444

L

L

G

N

P

P

D

D

Y

G

E

T

L

L

R

Q

L

L

V

-

A

-

P

-

E

E

E

T

V

L

G

P

A

T

A

L

I

F

T

D

D

E

E

K

I

T

A

R

V

L

T

L

L

A

I

I

T

T

E

H

V

V

D

S

Y

N

R

V

T

L

G

G

R

T

K

E

H

N

D

P

M

L

A

A

A

E

L

M

T

I

A

T

K

L

A

A

H

H

A

Q

N

H

G

G

V

A

V

K

T

V

V

L

W

V

D

D

L

G

A

A

H

Q

S

A

A

V

G

M

A

H

I

H

P

P

V

V

D

D

L

V

A

Q

G

A

A

L

K

D

A

C

D

D

F

F

A

Y

I

V

G

F

C

S

T

G

Y

H

K

K

Y

L

L

Y

N

-

G

-

G

G

P

P

G

T

A

G

P

I

A

G

F

I

I

L

Y

Y

V

V

A

-

P

-

R

-

H

-

A

K

D

E

M

A

A

L

L

L

P

Q

A

E

L

M

S

P

G

P

W

W

L

E

G

G

H

G

D

G

S

S

P

M

F

I

A

A

-

T

-

V

-

S

F

L

D

S

L

E

G

G

Y

T

K

T

A

W

G

T

V

K

G

A

I

P

S

W

R

R

M

F

R

E

V

A

G

G

T

T

P

P

P

N

V

T

L

G

Q

G

M

I

A

I

A

G

L

L

D

G

A

A

L

L

D

E

V

Y

W

V

D

S

D

A

V

L

D

G

L

L

N

N

D

N

L

I

R

A

T

E

R

Y

S

E

I

Q

D

N

L

L

S

M

E

H

R

Y

F

A

I

L

A

S

G

Q

V

L

E

E

A

-

T

S

C

V

P

P

D

D

L

L

T

T

L

L

A

Y

S

G

P

P

R

Q

N

N

-

R

-

L

-

G

-

V

-

I

A

A

A

F

E

N

R

L

G

G

S

K

Q

H

V

H

S

A

F

Y

-

D

-

V

-

G

K

S

F

F

E

L

E

D

G

N

Y

Y

A

G

A

I

M

A

Q

V

A

R

L

T

I

G

A

H

H

H

-
x
C

-

A

-

M

K

P

V

L

I

M

G

A

D

Y

F

Y

R

N

A

V

P

P

D

A

I

M

M

C

R
|
R

F

A

G

S

F

L

T

A

P

-

L

M

Y

Y

L

N

I

T

L

H

E

E

D

E

V

V

D

D

Q

R

A

L

V

V

E

T

R

G

L

L

A

Q

R

R

I

I

M

H

R

R

C364 (vs. gap) mutation to A: Abolishes activity towards L-cysteine but not towards selenocysteine.
R379 (= R356) mutation to A: Loss of function.

Sites not aligning to the query:

55 H→A: No effect.
123 H→A: Loss of function; possibly due to destabilization of PLP in the active site.

Q9Y697 Cysteine desulfurase; EC 2.8.1.7 from Homo sapiens (Human) (see 5 papers)
23% identity, 81% coverage: 18:340/397 of query aligns to 59:370/457 of Q9Y697

query
sites
Q9Y697

I

L

Y

Y

L

M

D

D

G

V

N

Q

S

A

L

T

G

T

P

P

L

L

-

D

P

P

K
x
R

S

V

A

L

A

D

A

A

R

M

V

L

A

P

Q

Y

T

L

V

I

A

N

S

Y

E

Y

W

G

G

N

D

P

H

H

L

S

I

R

T

T

-

H

-

A

-

Y

G

G

W

W

N

E

A

S

C

E

G

A

W

A

M

M

D

E

M

-

P

-

A

-

R

R

L

A

G

R

D

Q

Q

Q

I

V

G

A

R

S

L

L

I

I

G

G

A

A

E

D

A

P

G

R

H

E

V

I

V

F

-

T

-

S

G

G

D
x
A

T
|
T

L

E

S

S

I

N

K

N

V

I

F

A

Q

I

A

K

L

G

A

V

A

A

A

R

L

F

K

Y

L

R

N

S

P

R

H

K

R

K

R

H

V

L

I

I

L

T

S

T

D

Q

T

T

G

E

N

H

F

K

-

C

-

V

-

L

-

D

P

S

S

C

D

R

L

S

Y

L

M

E

A

A

E

E

G

G

L

F

I

Q

S

V

L

T

L

Y

G

L

P

P

D

V

Y

Q

E

K

L

S

R

G

L

I

V

I

A

D

P

L

E

K

E

E

V

L

G

E

A

A

I

I

T

Q

D

P

E

D

I

T

A

S

V

L

T

V

L

S

I

V

T

M

E

T

V

V

D

N

Y

N

R

E

T

I

G

G

R

V

K

K

H

Q

D

P

M

I

A

A

A

E

L

I

T

G

A

R

K

I

A

C

H

S

A

S

N

R

G

K

V

V

V

Y

T

F

V

H

W

T

D

D

L

A

A

A

H
x
Q

S

A

A

V

G

G

A

K

I

I

P

P

V

L

D

D

L

V

A

N

G

D

A

M

K

K

A

I

D

D

F

L

A

M

I

S

G

I

C
x
S

T

G

Y
x
H

K
|
K

Y

I

L

Y

N

-

G

-

G

G

P

P

G

K

A

G

P

V

A

G

F

A

I

I

Y

Y

V

I

A

R

P

R

R

R

H

-

A

-

D

-

M

-

A

-

L

-

P

-

A

-

L

-

S

-

G

-

W

-

L

-

G

-

H

-

D

-

S

-

P

P

F

R

A

V

F

R

D

V

L

E

G

A

Y

L

K

Q

A

S

G

G

V

G

G

G

I

Q

S

E

R

R

-

G

M

M

R

R

V

S

G

G

T
|
T

P

V

P

P

V

T

L

P

Q

L

M

V

A

V

A

G

L

L

D

G

A

A

L

C

D

E

V

V

W

A

D

Q

V

-

D

E

L

M

N

E

D

Y

L

D

R

H

T

K

R

R

S

I

I

S

D

K

L

L

S

S

E

E

R

R

F

L

I

I

A

Q

G

N

V

I

E

M

A

K

T

S

C

L

P

P

D

D

L

V

T

V

L

M

-

N

A

G

S

D

P

P

R

K

N

H

A

H

A

Y

E

P

R

G

G

C

S

I

Q

N

V

L

S

S

F

F

K

A

F

Y

E

V

E

E

G

G

Y

E

A

S

A

L

M

L

Q

M

A

A

L

L

R72 (≠ K30) to Q: in COXPD52; decreased protein expression in homozygous patient cells; dbSNP:rs200592030
A127 (≠ D83) binding pyridoxal 5'-phosphate
T128 (= T84) binding pyridoxal 5'-phosphate
Q235 (≠ H187) binding pyridoxal 5'-phosphate
S255 (≠ C207) binding pyridoxal 5'-phosphate
H257 (≠ Y209) binding pyridoxal 5'-phosphate
K258 (= K210) modified: N6-(pyridoxal phosphate)lysine
T295 (= T265) binding pyridoxal 5'-phosphate

Sites not aligning to the query:

381 binding Zn(2+)

6o13A E. Coli cysteine desulfurase sufs h123a with a cys-ketimine intermediate (see paper)
25% identity, 64% coverage: 127:381/397 of query aligns to 145:402/405 of 6o13A

query
sites
6o13A

L

L

G

N

P

P

D

D

Y

G

E

T

L

L

R

Q

L

L

V

-

A

-

P

-

E

E

E

T

V

L

G

P

A

T

A

L

I

F

T

D

D

E

E

K

I

T

A

R

V

L

T

L

L

A

I

I

T

T

E

H

V

V

D

S

Y
x
N

R

V

T

L

G

G

R

T

K

E

H

N

D

P

M

L

A

A

A

E

L

M

T

I

A

T

K

L

A

A

H

H

A

Q

N

H

G

G

V

A

V

K

T

V

V

L

W

V

D
|
D

L

G

A
|
A

H
x
Q

S

A

A

V

G

M

A

H

I

H

P

P

V

V

D

D

L

V

A

Q

G

A

A

L

K

D

A

C

D

D

F

F

A

Y

I

V

G

F

C
x
S

T

G

Y
x
H

K
|
K

Y

L

L

Y

N

-

G

-

G

G

P

P

G

T

A

G

P

I

A

G

F

I

I

L

Y

Y

V

V

A

-

P

-

R

-

H

-

A

K

D

E

M

A

A

L

L

L

P

Q

A

E

L

M

S

P

G

P

W

W

L

E

G

G

H

G

D

G

S

S

P

M

F

I

A

A

-

T

-

V

-

S

F

L

D

S

L

E

G

G

Y

T

K

T

A

W

G

T

V

K

G

A

I

P

S

W

R

R

M

F

R

E

V

A

G

G

T

T

P

P

P

N

V

T

L

G

Q

G

M

I

A

I

A

G

L

L

D

G

A

A

L

L

D

E

V

Y

W

V

D

S

D

A

V

L

D

G

L

L

N

N

D

N

L

I

R

A

T

E

R

Y

S

E

I

Q

D

N

L

L

S

M

E

H

R

Y

F

A

I

L

A

S

G

Q

V

L

E

E

A

-

T

S

C

V

P

P

D

D

L

L

T

T

L

L

A

Y

S

G

P

P

R

Q

N

N

-

R

-

L

-

G

-

V

-

I

A

A

A

F

E

N

R

L

G

G

S

K

Q

H

V

H

S

A

F

Y

-

D

-

V

-

G

K

S

F

F

E

L

E

D

G

N

Y

Y

A

G

A

I

M

A

Q

V

A

R

L

T

I

G

A

H

H

H

-
x
C

-

A

-

M

K

P

V

L

I

M

G

A

D

Y

F

Y

R

N

A

V

P

P

D

A

I

M

M

C

R
|
R

F

A

G

S

F

L

T

A

P

-

L

M

Y

Y

L

N

I

T

L

H

E

E

D

E

V

V

D

D

Q

R

A

L

V

V

E

T

R

G

L

L

A

Q

R

R

I

I

M

H

R

R

binding Cys-ketimine: N174 (≠ Y159), D199 (= D184), A201 (= A186), Q202 (≠ H187), S222 (≠ C207), H224 (≠ Y209), K225 (= K210), C363 (vs. gap), R378 (= R356)

Sites not aligning to the query:

binding Cys-ketimine: 93, 94

1kmkA E. Coli nifs/csdb protein at 2.20a with the cysteine perselenide intermediate (residue csz). (see paper)
25% identity, 64% coverage: 127:381/397 of query aligns to 145:402/405 of 1kmkA

query
sites
1kmkA

L

L

G

N

P

P

D

D

Y

G

E

T

L

L

R

Q

L

L

V

-

A

-

P

-

E

E

E

T

V

L

G

P

A

T

A

L

I

F

T

D

D

E

E

K

I

T

A

R

V

L

T

L

L

A

I

I

T

T

E

H

V

V

D

S

Y
x
N

R

V

T

L

G

G

R

T

K

E

H

N

D

P

M

L

A

A

A

E

L

M

T

I

A

T

K

L

A

A

H

H

A

Q

N

H

G

G

V

A

V

K

T

V

V

L

W

V

D
|
D

L

G

A
|
A

H
x
Q

S

A

A

V

G

M

A

H

I

H

P

P

V

V

D

D

L

V

A

Q

G

A

A

L

K

D

A

C

D

D

F

F

A

Y

I

V

G

F

C
x
S

T

G

Y
x
H

K
|
K

Y

L

L

Y

N

-

G

-

G

G

P

P

G

T

A

G

P

I

A

G

F

I

I

L

Y

Y

V

V

A

-

P

-

R

-

H

-

A

K

D

E

M

A

A

L

L

L

P

Q

A

E

L

M

S

P

G

P

W

W

L

E

G

G

H

G

D

G

S

S

P

M

F

I

A

A

-

T

-

V

-

S

F

L

D

S

L

E

G

G

Y

T

K

T

A

W

G

T

V

K

G

A

I

P

S

W

R

R

M

F

R

E

V

A

G

G

T

T

P

P

P

N

V

T

L

G

Q

G

M

I

A

I

A

G

L

L

D

G

A

A

L

L

D

E

V

Y

W

V

D

S

D

A

V

L

D

G

L

L

N

N

D

N

L

I

R

A

T

E

R

Y

S

E

I

Q

D

N

L

L

S

M

E

H

R

Y

F

A

I

L

A

S

G

Q

V

L

E

E

A

-

T

S

C

V

P

P

D

D

L

L

T

T

L

L

A

Y

S

G

P

P

R

Q

N

N

-

R

-

L

-

G

-

V

-

I

A

A

A

F

E

N

R

L

G

G

S

K

Q

H

V

H

S

A

F

Y

-

D

-

V

-

G

K

S

F

F

E

L

E

D

G

N

Y

Y

A

G

A

I

M

A

Q

V

A
x
R

L

T

I

G

A

H

H

H

-
x
C

-

A

-

M

K

P

V

L

I

M

G

A

D

Y

F

Y

R

N

A

V

P

P

D

A

I

M

M

C

R
|
R

F

A

G

S

F

L

T

A

P

-

L

M

Y

Y

L

N

I

T

L

H

E

E

D

E

V

V

D

D

Q

R

A

L

V

V

E

T

R

G

L

L

A

Q

R

R

I

I

M

H

R

R

active site: D199 (= D184), A201 (= A186), Q202 (≠ H187), K225 (= K210), R358 (≠ A339), C363 (vs. gap)
binding pyridoxal-5'-phosphate: D199 (= D184), A201 (= A186), Q202 (≠ H187), S222 (≠ C207), H224 (≠ Y209), K225 (= K210)
binding selenocysteine: N174 (≠ Y159), K225 (= K210), R358 (≠ A339), C363 (vs. gap), R378 (= R356)

Sites not aligning to the query:

active site: 122
binding pyridoxal-5'-phosphate: 93, 94, 122
binding selenocysteine: 122

1i29A Crystal structure of csdb complexed with l-propargylglycine (see paper)
25% identity, 64% coverage: 127:381/397 of query aligns to 145:402/405 of 1i29A

query
sites
1i29A

L

L

G

N

P

P

D

D

Y

G

E

T

L

L

R

Q

L

L

V

-

A

-

P

-

E

E

E

T

V

L

G

P

A

T

A

L

I

F

T

D

D

E

E

K

I

T

A

R

V

L

T

L

L

A

I

I

T

T

E

H

V

V

D

S

Y

N

R

V

T

L

G

G

R

T

K

E

H

N

D

P

M

L

A

A

A

E

L

M

T

I

A

T

K

L

A

A

H

H

A

Q

N

H

G

G

V

A

V

K

T

V

V

L

W

V

D
|
D

L

G

A
|
A

H
x
Q

S

A

A

V

G

M

A

H

I

H

P

P

V

V

D

D

L

V

A

Q

G

A

A

L

K

D

A

C

D

D

F

F

A

Y

I

V

G

F

C
x
S

T

G

Y
x
H

K
|
K

Y

L

L

Y

N

-

G

-

G

G

P

P

G

T

A

G

P

I

A

G

F

I

I

L

Y

Y

V

V

A

-

P

-

R

-

H

-

A

K

D

E

M

A

A

L

L

L

P

Q

A

E

L

M

S

P

G

P

W

W

L

E

G

G

H

G

D

G

S

S

P

M

F

I

A

A

-

T

-

V

-

S

F

L

D

S

L

E

G

G

Y

T

K

T

A

W

G

T

V

K

G

A

I

P

S

W

R

R

M

F

R

E

V

A

G

G

T

T

P

P

P

N

V

T

L

G

Q

G

M

I

A

I

A

G

L

L

D

G

A

A

L

L

D

E

V

Y

W

V

D

S

D

A

V

L

D

G

L

L

N

N

D

N

L

I

R

A

T

E

R

Y

S

E

I

Q

D

N

L

L

S

M

E

H

R

Y

F

A

I

L

A

S

G

Q

V

L

E

E

A

-

T

S

C

V

P

P

D

D

L

L

T

T

L

L

A

Y

S

G

P

P

R

Q

N

N

-

R

-

L

-

G

-

V

-

I

A

A

A

F

E

N

R

L

G

G

S

K

Q

H

V

H

S

A

F

Y

-

D

-

V

-

G

K

S

F

F

E

L

E

D

G

N

Y

Y

A

G

A

I

M

A

Q

V

A
x
R

L

T

I

G

A

H

H

H

-
x
C

-

A

-

M

K

P

V

L

I

M

G

A

D

Y

F

Y

R

N

A

V

P

P

D

A

I

M

M

C

R

R

F

A

G

S

F

L

T

A

P

-

L

M

Y

Y

L

N

I

T

L

H

E

E

D

E

V

V

D

D

Q

R

A

L

V

V

E

T

R

G

L

L

A

Q

R

R

I

I

M

H

R

R

active site: D199 (= D184), A201 (= A186), Q202 (≠ H187), K225 (= K210), R358 (≠ A339), C363 (vs. gap)
binding pyridoxal-5'-phosphate: D199 (= D184), A201 (= A186), S222 (≠ C207), H224 (≠ Y209), K225 (= K210)

Sites not aligning to the query:

active site: 122
binding (2S)-2-aminobut-3-ynoic acid: 30, 122
binding pyridoxal-5'-phosphate: 93, 94, 122

8tvtA Structure of human cysteine desulfurase nfs1 with l-propargylglycine bound to active site plp in complex with isd11, acp1 and iscu2
23% identity, 81% coverage: 18:340/397 of query aligns to 5:316/400 of 8tvtA

query
sites
8tvtA

I

L

Y

Y

L

M

D

D

G

V

N
x
Q

S
x
A

L

T

G

T

P

P

L

L

-

D

P

P

K

R

S

V

A

L

A

D

A

A

R

M

V

L

A

P

Q

Y

T

L

V

I

A

N

S

Y

E

Y

W

G

G

N

D

P

H

H

L

S

I

R

T

T

-

H

-

A

-

Y

G

G

W

W

N

E

A

S

C

E

G

A

W

A

M

M

D

E

M

-

P

-

A

-

R

R

L

A

G

R

D

Q

Q

Q

I

V

G

A

R

S

L

L

I

I

G

G

A

A

E

D

A

P

G

R

H

E

V

I

V

F

-

T

-

S

G
|
G

D
x
A

T
|
T

L

E

S

S

I

N

K

N

V

I

F

A

Q

I

A

K

L

G

A

V

A

A

A

R

L

F

K

Y

L

R

N

S

P

R

H

K

R

K

R

H

V

L

I

I

L

T

S

T

D

Q

T

T

G

E

N
x
H

F

K

-

C

-

V

-

L

-

D

P

S

S

C

D

R

L

S

Y

L

M

E

A

A

E

E

G

G

L

F

I

Q

S

V

L

T

L

Y

G

L

P

P

D

V

Y

Q

E

K

L

S

R

G

L

I

V

I

A

D

P

L

E

K

E

E

V

L

G

E

A

A

I

I

T

Q

D

P

E

D

I

T

A

S

V

L

T

V

L

S

I

V

T

M

E

T

V

V

D

N

Y
x
N

R

E

T

I

G

G

R

V

K

K

H

Q

D

P

M

I

A

A

A

E

L

I

T

G

A

R

K

I

A

C

H

S

A

S

N

R

G

K

V

V

V

Y

T

F

V

H

W

T

D
|
D

L

A

A

A

H
x
Q

S

A

A

V

G

G

A

K

I

I

P

P

V

L

D

D

L

V

A

N

G

D

A

M

K

K

A

I

D

D

F

L

A

M

I

S

G

I

C
x
S

T

G

Y
x
H

K
|
K

Y

I

L

Y

N

-

G

-

G

G

P

P

G

K

A

G

P

V

A

G

F

A

I

I

Y

Y

V

I

A

R

P

R

R

R

H

-

A

-

D

-

M

-

A

-

L

-

P

-

A

-

L

-

S

-

G

-

W

-

L

-

G

-

H

-

D

-

S

-

P

P

F

R

A

V

F

R

D

V

L

E

G

A

Y

L

K

Q

A

S

G

G

V

G

G

G

I

Q

S

E

R

R

-

G

M

M

R

R

V

S

G

G

T

T

P

V

P

P

V

T

L

P

Q

L

M

V

A

V

A

G

L

L

D

G

A

A

L

C

D

E

V

V

W

A

D

Q

V

-

D

E

L

M

N

E

D

Y

L

D

R

H

T

K

R

R

S

I

I

S

D

K

L

L

S

S

E

E

R

R

F

L

I

I

A

Q

G

N

V

I

E

M

A

K

T

S

C

L

P

P

D

D

L

V

T

V

L

M

-

N

A

G

S

D

P

P

R

K

N

H

A

H

A

Y

E

P

R

G

G

C

S

I

Q

N

V

L

S

S

F

F

K

A

F

Y

E

V

E

E

G

G

Y

E

A

S

A

L

M

L

Q

M

A

A

L

L

binding L-Propargylglycine: Q10 (≠ N23), A11 (≠ S24), H102 (≠ N112), N153 (≠ Y159), H203 (≠ Y209), K204 (= K210)
binding pyridoxal-5'-phosphate: G72 (= G82), A73 (≠ D83), T74 (= T84), H102 (≠ N112), D178 (= D184), Q181 (≠ H187), S201 (≠ C207), H203 (≠ Y209), K204 (= K210)

Sites not aligning to the query:

binding L-Propargylglycine: 325, 327, 353

6nzuA Structure of the human frataxin-bound iron-sulfur cluster assembly complex (see paper)
23% identity, 81% coverage: 18:340/397 of query aligns to 4:315/402 of 6nzuA

query
sites
6nzuA

I

L

Y

Y

L

M

D

D

G

V

N

Q

S

A

L

T

G

T

P

P

L

L

-

D

P

P

K

R

S

V

A

L

A

D

A

A

R

M

V

L

A

P

Q

Y

T

L

V

I

A

N

S

Y

E

Y

W

G

G

N

D

P

H

H

L

S

I

R

T

T

-

H

-

A

-

Y

G

G

W

W

N

E

A

S

C

E

G

A

W

A

M

M

D

E

M

-

P

-

A

-

R

R

L

A

G

R

D

Q

Q

Q

I

V

G

A

R

S

L

L

I

I

G

G

A

A

E

D

A

P

G

R

H

E

V

I

V

F

-

T

-

S

G

G

D
x
A

T
|
T

L

E

S

S

I

N

K

N

V

I

F

A

Q

I

A

K

L

G

A

V

A

A

A

R

L

F

K

Y

L

R

N

S

P

R

H

K

R

K

R

H

V

L

I

I

L

T

S

T

D

Q

T

T

G

E

N

H

F

K

-

C

-

V

-

L

-

D

P

S

S

C

D

R

L

S

Y

L

M

E

A

A

E

E

G

G

L

F

I

Q

S

V

L

T

L

Y

G

L

P

P

D

V

Y

Q

E

K

L

S

R

G

L

I

V

I

A

D

P

L

E

K

E

E

V

L

G

E

A

A

I

I

T

Q

D

P

E

D

I

T

A

S

V

L

T

V

L

S

I

V

T

M

E

T

V

V

D

N

Y

N

R

E

T

I

G

G

R

V

K

K

H

Q

D

P

M

I

A

A

A

E

L

I

T

G

A

R

K

I

A

C

H

S

A

S

N

R

G

K

V

V

V

Y

T

F

V

H

W

T

D
|
D

L

A

A

A

H

Q

S

A

A

V

G

G

A

K

I

I

P

P

V

L

D

D

L

V

A

N

G

D

A

M

K

K

A

I

D

D

F

L

A

M

I

S

G

I

C

S

T

G

Y
x
H

K
|
K

Y

I

L

Y

N

-

G

-

G

G

P

P

G

K

A

G

P

V

A

G

F

A

I

I

Y

Y

V

I

A

R

P

R

R

R

H

-

A

-

D

-

M

-

A

-

L

-

P

-

A

-

L

-

S

-

G

-

W

-

L

-

G

-

H

-

D

-

S

-

P

P

F

R

A

V

F

R

D

V

L

E

G

A

Y

L

K

Q

A

S

G

G

V

G

G

G

I

Q

S

E

R

R

-

G

M

M

R

R

V

S

G

G

T

T

P

V

P

P

V

T

L

P

Q

L

M

V

A

V

A

G

L

L

D

G

A

A

L

C

D

E

V

V

W

A

D

Q

V

-

D

E

L

M

N

E

D

Y

L

D

R

H

T

K

R

R

S

I

I

S

D

K

L

L

S

S

E

E

R

R

F

L

I

I

A

Q

G

N

V

I

E

M

A

K

T

S

C

L

P

P

D

D

L

V

T

V

L

M

-

N

A

G

S

D

P

P

R

K

N

H

A

H

A

Y

E

P

R

G

G

C

S

I

Q

N

V

L

S

S

F

F

K

A

F

Y

E

V

E

E

G

G

Y

E

A

S

A

L

M

L

Q

M

A

A

L

L

binding pyridoxal-5'-phosphate: A72 (≠ D83), T73 (= T84), D177 (= D184), H202 (≠ Y209), K203 (= K210)

P96060 2-aminoethylphosphonate--pyruvate transaminase; 2-aminoethylphosphonate aminotransferase; AEP transaminase; AEPT; EC 2.6.1.37 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
25% identity, 50% coverage: 52:250/397 of query aligns to 26:226/367 of P96060

query
sites
P96060

W

F

N

D

A

S

C

C

G

T

W

W

M

D

D

D

-

D

-

Y

-

N

-

I

-

G

M

V

P

V

A

E

R

Q

L

I

G

R

D

Q

Q

Q

I

L

G

T

R

A

L

L

I

A

G

T

A

A

E

S

A

E

G

G

H

Y

-

T

-

S

V

V

V

L

G

Q

D

G

T

S

L

G

S

S

I

Y

K

A

V

V

F

E

Q

A

A

V

L

L

A

G

A

S

A

A

L

L

K

-

L

-

N

G

P

P

H

Q

R

D

R

K

V

V

I

L

L

I

S

V

D

S

T

N

G

G

N

A

F

Y

P

G

S

A

D

R

L

M

Y

V

M

E

A

M

E

A

G

G

L

L

I

M

S

G

L

I

L

A

G

H

P

H

D

A

Y

Y

E

D

L

C

R

G

L

E

V

V

A

A

P

R

E

P

E

D

V

V

G

Q

A

A

-

I

-

D

A

A

I

I

T

L

D

N

-

A

-

D

-

P

E

T

I

I

A

S

V

H

T

I

L

A

I

M

T

V

E

H

V

S

D

E

Y

T

R

T

T

T

G

G

R

M

K

L

H

N

D

P

M

I

A

D

A

E

L

V

T

G

A

A

K

L

A

A

H

H

A

R

N

Y

G

G

V

K

V

T

T

Y

V

I

W

V

D
|
D

L

A

A

M

H

S

S

S

A

F

G

G

A

G

I

I

P

P

V

M

D

D

L

I

A

A

G

A

A

L

K

H

A

I

D

D

F

Y

A

L

I

I

G

S

C

S

T

A

Y

N

K
|
K

Y

C

L

I

N

Q

G

G

G

V

P

P

G

G

A

-

P

-

A

-

F

F

I

A

Y

F

V

V

A

I

P

A

R

R

H

E

A

Q

D

K

M

L

A

A

L

-

P

-

A

-

L

-

S

-

G

A

W

C

L

K

G

G

H

H

D

S

S

R

P

S

F

L

A

S

F

L

D

D

L

L

D168 (= D184) mutation to A: Loss of enzymatic activity.
K194 (= K210) mutation K->L,R: Loss of enzymatic activity.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
340 mutation R->A,K: Decreased affinity for all of the substrates.

Query Sequence

>WP_085640330.1 NCBI__GCF_002115805.1:WP_085640330.1
MTDFTATRAAFHLPEGMIYLDGNSLGPLPKSAAARVAQTVASEWGDHLITGWNACGWMDM
PARLGDQIGRLIGAEAGHVVVGDTLSIKVFQALAAALKLNPHRRVILSDTGNFPSDLYMA
EGLISLLGPDYELRLVAPEEVGAAITDEIAVTLITEVDYRTGRKHDMAALTAKAHANGVV
TVWDLAHSAGAIPVDLAGAKADFAIGCTYKYLNGGPGAPAFIYVAPRHADMALPALSGWL
GHDSPFAFDLGYKAGVGISRMRVGTPPVLQMAALDAALDVWDDVDLNDLRTRSIDLSERF
IAGVEATCPDLTLASPRNAAERGSQVSFKFEEGYAAMQALIAHKVIGDFRAPDIMRFGFT
PLYLILEDVDQAVERLARIMRDRLWDTPEFKQRKAVT

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory