SitesBLAST
Comparing WP_085640964.1 NCBI__GCF_002115805.1:WP_085640964.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
9br7C Succinate--hydroxymethylglutarate CoA-transferase (see paper)
41% identity, 87% coverage: 3:335/383 of query aligns to 4:338/403 of 9br7C
O06644 Formyl-CoA:oxalate CoA-transferase; FCOCT; Formyl-coenzyme A transferase; EC 2.8.3.16 from Oxalobacter formigenes (see 4 papers)
33% identity, 99% coverage: 1:379/383 of query aligns to 1:412/428 of O06644
- Q17 (≠ L17) mutation to A: 45-fold decrease of the catalytic effiency.
- R38 (= R38) binding CoA
- W48 (≠ F48) mutation to F: Little change in the affinity binding and catalytic efficiency, and it does not display major structural changes.; mutation to P: Little change in the affinity binding and catalytic efficiency. It exhibits substrate inhibition with oxalate. It does not display major structural changes.
- R104 (= R103) binding CoA
- D169 (= D168) active site, Nucleophile; mutation to A: Loss of CoA-transferase activity.; mutation to E: Loss of CoA-transferase activity.; mutation to S: Loss of CoA-transferase activity.
- G259 (vs. gap) mutation to A: 2.5-fold decrease of the catalytic effiency.
- G260 (vs. gap) mutation to A: 25-fold decrease of the catalytic effiency. Reduction of the affinity binding for both formyl-CoA and oxalate.
1p5rA Formyl-coa transferase in complex with coenzyme a (see paper)
32% identity, 99% coverage: 2:379/383 of query aligns to 1:411/427 of 1p5rA
- active site: Q16 (≠ L17), E139 (≠ D139), D168 (= D168), G259 (vs. gap), G260 (vs. gap)
- binding coenzyme a: H14 (= H15), V15 (= V16), Q16 (≠ L17), A17 (= A18), R37 (= R38), M73 (≠ L73), K74 (= K74), N95 (= N95), F96 (≠ Y96), A100 (≠ V100), R103 (= R103), K136 (≠ P136), V137 (≠ A137), D168 (= D168), M199 (= M199)
2vjoA Formyl-coa transferase mutant variant q17a with aspartyl-coa thioester intermediates and oxalate (see paper)
32% identity, 99% coverage: 2:379/383 of query aligns to 1:411/427 of 2vjoA
- active site: A16 (≠ L17), E139 (≠ D139), D168 (= D168), G259 (vs. gap), G260 (vs. gap)
- binding coenzyme a: H14 (= H15), A16 (≠ L17), A17 (= A18), R37 (= R38), L71 (= L71), M73 (≠ L73), N95 (= N95), F96 (≠ Y96), G97 (≠ R97), R103 (= R103), M104 (= M104), K136 (≠ P136), V137 (≠ A137), Y138 (= Y138), D168 (= D168), M199 (= M199)
- binding oxalate ion: G257 (vs. gap), G259 (vs. gap), Q261 (vs. gap)
2vjkA Formyl-coa transferase with aspartyl-coa thioester intermediate derived from oxalyl-coa (see paper)
32% identity, 99% coverage: 2:379/383 of query aligns to 1:411/427 of 2vjkA
- active site: Q16 (≠ L17), E139 (≠ D139), D168 (= D168), G259 (vs. gap), G260 (vs. gap)
- binding coenzyme a: H14 (= H15), Q16 (≠ L17), A17 (= A18), R37 (= R38), M73 (≠ L73), K74 (= K74), N95 (= N95), F96 (≠ Y96), G97 (≠ R97), R103 (= R103), M104 (= M104), K136 (≠ P136), V137 (≠ A137), Y138 (= Y138), D168 (= D168), M199 (= M199)
- binding magnesium ion: D293 (≠ V259), D296 (= D262)
1t4cA Formyl-coa transferase in complex with oxalyl-coa (see paper)
32% identity, 99% coverage: 2:379/383 of query aligns to 1:411/427 of 1t4cA
- active site: Q16 (≠ L17), E139 (≠ D139), D168 (= D168), G259 (vs. gap), G260 (vs. gap)
- binding coenzyme a: H14 (= H15), V15 (= V16), Q16 (≠ L17), R37 (= R38), M73 (≠ L73), N95 (= N95), F96 (≠ Y96), R103 (= R103), M104 (= M104), V137 (≠ A137), Y138 (= Y138), D168 (= D168), M199 (= M199)
- binding oxalic acid: G259 (vs. gap), G260 (vs. gap)
1t3zA Formyl-coa tranferase mutant asp169 to ser (see paper)
32% identity, 99% coverage: 2:379/383 of query aligns to 1:411/427 of 1t3zA
- active site: Q16 (≠ L17), E139 (≠ D139), S168 (≠ D168), G259 (vs. gap), G260 (vs. gap)
- binding oxidized coenzyme a: H14 (= H15), V15 (= V16), A17 (= A18), R37 (= R38), K74 (= K74), N95 (= N95), F96 (≠ Y96), A100 (≠ V100), R103 (= R103), M104 (= M104), K136 (≠ P136), V137 (≠ A137), Y138 (= Y138), E139 (≠ D139), M199 (= M199)
3ubmB Formyl-coa:oxalate coa-transferase from acetobacter aceti (see paper)
33% identity, 98% coverage: 2:376/383 of query aligns to 2:409/430 of 3ubmB
- active site: Q17 (≠ L17), E140 (≠ D139), D182 (= D168), G261 (vs. gap), G262 (vs. gap)
- binding coenzyme a: V16 (= V16), R38 (= R38), L72 (= L71), N73 (≠ D72), T74 (≠ L73), K75 (= K74), N96 (= N95), F97 (≠ Y96), R98 (= R97), A101 (≠ V100), R104 (= R103), K125 (≠ S124), D182 (= D168), M213 (= M199)
P69902 Formyl-CoA:oxalate CoA-transferase; FCOCT; Formyl-coenzyme A transferase; Formyl-CoA transferase; EC 2.8.3.16 from Escherichia coli (strain K12) (see paper)
31% identity, 99% coverage: 1:379/383 of query aligns to 1:400/416 of P69902
1q6yA Hypothetical protein yfdw from e. Coli bound to coenzyme a (see paper)
31% identity, 99% coverage: 1:379/383 of query aligns to 1:400/417 of 1q6yA
- active site: Q17 (≠ L17), E140 (≠ D139), D169 (= D168), G248 (vs. gap), G249 (vs. gap)
- binding coenzyme a: V16 (= V16), Q17 (≠ L17), S18 (≠ A18), R38 (= R38), L72 (= L71), N73 (≠ D72), T74 (≠ L73), K75 (= K74), N96 (= N95), F97 (≠ Y96), H98 (≠ R97), M105 (= M104), I124 (≠ V123), K137 (≠ P136), A138 (= A137), Y139 (= Y138), D169 (= D168), M200 (= M199)
1pt5A Crystal structure of gene yfdw of e. Coli (see paper)
31% identity, 99% coverage: 2:379/383 of query aligns to 1:399/415 of 1pt5A
- active site: Q16 (≠ L17), E139 (≠ D139), D168 (= D168), G247 (vs. gap), G248 (vs. gap)
- binding acetyl coenzyme *a: V15 (= V16), S17 (≠ A18), R37 (= R38), L71 (= L71), N72 (≠ D72), T73 (≠ L73), K74 (= K74), N95 (= N95), F96 (≠ Y96), H97 (≠ R97), K124 (≠ S124), K136 (≠ P136), A137 (= A137), Y138 (= Y138), E139 (≠ D139), D168 (= D168), M199 (= M199)
1q7eA Crystal structure of yfdw protein from e. Coli (see paper)
30% identity, 99% coverage: 1:379/383 of query aligns to 1:393/410 of 1q7eA