Comparing WP_085770443.1 NCBI__GCF_002117405.1:WP_085770443.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P13009 Methionine synthase; 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12-dependent; MS; EC 2.1.1.13 from Escherichia coli (strain K12) (see 5 papers)
60% identity, 99% coverage: 10:1229/1238 of query aligns to 3:1224/1227 of P13009
Sites not aligning to the query:
Q99707 Methionine synthase; MS; 5-methyltetrahydrofolate--homocysteine methyltransferase; Cobalamin-dependent methionine synthase; Vitamin-B12 dependent methionine synthase; EC 2.1.1.13 from Homo sapiens (Human) (see 6 papers)
55% identity, 98% coverage: 20:1229/1238 of query aligns to 27:1262/1265 of Q99707
4cczA Crystal structure of human 5-methyltetrahydrofolate-homocysteine methyltransferase, the homocysteine and folate binding domains
57% identity, 50% coverage: 21:642/1238 of query aligns to 12:611/611 of 4cczA
3bulA E. Coli i690c/g743c meth c-terminal fragment (649-1227) (see paper)
58% identity, 46% coverage: 656:1229/1238 of query aligns to 2:574/577 of 3bulA
3ivaA Structure of the b12-dependent methionine synthase (meth) c-teminal half with adohcy bound (see paper)
58% identity, 46% coverage: 656:1229/1238 of query aligns to 2:574/576 of 3ivaA
8g3hA Structure of cobalamin-dependent methionine synthase (meth) in a resting state (see paper)
36% identity, 67% coverage: 20:846/1238 of query aligns to 10:806/841 of 8g3hA
Sites not aligning to the query:
3k13C Structure of the pterin-binding domain metr of 5- methyltetrahydrofolate-homocysteine methyltransferase from bacteroides thetaiotaomicron
67% identity, 23% coverage: 359:645/1238 of query aligns to 1:286/287 of 3k13C
6bdyA Crystal structure of the meth reactivation domain bound to sinefungin (see paper)
52% identity, 26% coverage: 908:1229/1238 of query aligns to 4:324/326 of 6bdyA
1mskA Methionine synthase (activation domain) (see paper)
52% identity, 26% coverage: 908:1229/1238 of query aligns to 4:324/327 of 1mskA
1bmtA How a protein binds b12: a 3.O angstrom x-ray structure of the b12- binding domains of methionine synthase (see paper)
67% identity, 20% coverage: 656:900/1238 of query aligns to 2:246/246 of 1bmtA
8sseA Methionine synthase, c-terminal fragment, cobalamin and reactivation domains from thermus thermophilus hb8 (see paper)
33% identity, 43% coverage: 665:1197/1238 of query aligns to 4:506/507 of 8sseA
1q8jA Cobalamin-dependent methionine synthase (1-566) from thermotoga maritima (cd2+, hcy, methyltetrahydrofolate complex) (see paper)
28% identity, 47% coverage: 20:604/1238 of query aligns to 12:538/559 of 1q8jA
3bofA Cobalamin-dependent methionine synthase (1-566) from thermotoga maritima complexed with zn2+ and homocysteine (see paper)
28% identity, 47% coverage: 20:604/1238 of query aligns to 12:538/560 of 3bofA
5vooA Methionine synthase folate-binding domain with methyltetrahydrofolate from thermus thermophilus hb8 (see paper)
35% identity, 23% coverage: 365:646/1238 of query aligns to 5:281/282 of 5vooA
7xcnP Crystal structure of the mttb-mttc complex at 2.7 a resolution (see paper)
34% identity, 13% coverage: 685:843/1238 of query aligns to 28:188/215 of 7xcnP
Sites not aligning to the query:
3ezxA Structure of methanosarcina barkeri monomethylamine corrinoid protein
35% identity, 14% coverage: 666:842/1238 of query aligns to 8:185/212 of 3ezxA
Sites not aligning to the query:
2i2xB Crystal structure of methanol:cobalamin methyltransferase complex mtabc from methanosarcina barkeri (see paper)
29% identity, 17% coverage: 670:879/1238 of query aligns to 46:245/258 of 2i2xB
Q46EH4 Methanol--corrinoid protein; Methanol:corrinoid methyltransferase 1 subunit of 27 kDa; MT1 subunit 27 kDa from Methanosarcina barkeri (strain Fusaro / DSM 804) (see paper)
29% identity, 17% coverage: 670:879/1238 of query aligns to 46:245/258 of Q46EH4
Sites not aligning to the query:
4jgiB 1.5 angstrom crystal structure of a novel cobalamin-binding protein from desulfitobacterium hafniense dcb-2 (see paper)
33% identity, 14% coverage: 704:873/1238 of query aligns to 45:202/206 of 4jgiB
1y80A Structure of a corrinoid (factor iiim)-binding protein from moorella thermoacetica
38% identity, 8% coverage: 749:843/1238 of query aligns to 3:99/125 of 1y80A
Sites not aligning to the query:
>WP_085770443.1 NCBI__GCF_002117405.1:WP_085770443.1
MTFDKAHGPSILEALETRASERILILDGAMGTMVQRHKFSEEDFRGERFKDHHKDLRGNN
DLLIFTQPEAILEIHRQYLRAGADIIETNTFSATTIAQADYGLEAIVSELNREGARLARR
ACEEIGRETGIARFVAGSIGPTNRTASISPDVSNPGFRAVSFDELRAAYKQAALGLIEGG
ADILLVETIFDTLNAKAAIAALEDAFDEVGTRFPIMISGTITDLSGRTLSGQTARAFWHS
LAHARPFSIGFNCALGAKEMRQHIVEIGRAADTRVCAFPNAGLPNEFGLYDESPEYMAEL
VGEFARSGLVNVLGGCCGTTPDHIAAIAKAAQGVAPREIPEIPPLLRLSGLEPFTLTSDI
PFVNVGERTNVTGSAKFRKLITAGEYAAALDVARDQVANGAQVIDVNMDEGLLDSKQAMV
DFLNLVAAEPDIARVPVMVDSSKFEVIEAGLKCLQGKGVVNSISLKEGEEKFRDEARKVS
RYGAAMVVMAFDEKGQADTFERKVEIAKRAYKILVEEVGFPPQDIIFDPNIFAVATGIEE
HENYGVDFIEATKAIKEAMPLVHVSGGVSNLSFSFRGNEPVREAMHSVFLYHAIQAGMDM
GIVNAGQLAVYEKIDPELREACEDVVLNRRKDGTERLVTLAEKFKGAGAKREEKDAAWRE
ASVEKRLEYALVNGVADYIEADVEEARIASTRPLDVIEGPLMAGMNVVGDLFGQGKMFLP
QVVKSARVMKQAVAHLLPYMEKDKDQRSSAGKILLATVKGDVHDIGKNIVGVVLGCNNFE
VIDLGVMTPAAKIIEMARNEKVDLIGLSGLITPSLDEMCFVASELEREGFETPLLIGGAT
TSRVHTAVKISPNYRRGQAVYVTDASRAVGVAQALLSATRGDYKAKTRDEYERVAEVHAK
AQADKNRVPIAAARANAAKLDWESYTPTKPTFTGVRGFASYDVAELIPYIDWTPFFSTWE
FKGRFPALLDDPARGEAARTLYDDARAMLDRIVAERWFTPKAAIGFWPANSVGDDIALYT
GESRQERLATLYTLRQQLPRQGARPNLALADFVAPEGSGKADYIGAFVVTAGAEEAKISA
RYARANDDYGSIMVKALADRIVEALAERMHERVRREFWGYAAAENLAPQELMGEPYQGIR
PAPGYPAQPDHSEKATLFRLLSAERTGAKLTETFAMSPPSTICGVYIAHPEAHYFGVSKV
ERDQVEDYARRKGVPVAEMERWLAPVLNYAREVEAAAE
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SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.
Lawrence Berkeley National Laboratory