SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_085770473.1 NCBI__GCF_002117405.1:WP_085770473.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P9WP55 O-acetylserine sulfhydrylase; OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase; EC 2.5.1.47 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
63% identity, 94% coverage: 18:323/326 of query aligns to 3:305/310 of P9WP55

query
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P9WP55

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K44 (= K61) modified: N6-(pyridoxal phosphate)lysine
N74 (= N92) binding pyridoxal 5'-phosphate
GTGGT 178:182 (= GTGGT 196:200) binding pyridoxal 5'-phosphate
S266 (= S284) binding pyridoxal 5'-phosphate

2q3dA 2.2 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) from mycobacterium tuberculosis in complex with the reaction intermediate alpha-aminoacrylate (see paper)
63% identity, 94% coverage: 18:323/326 of query aligns to 3:305/306 of 2q3dA

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2q3dA

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active site: K44 (= K61), S266 (= S284), P293 (= P311)
binding 2-[(3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl)-amino]-propionic acid: K44 (= K61), T71 (= T89), S72 (= S90), N74 (= N92), T75 (= T93), Q144 (= Q162), V177 (= V195), G178 (= G196), T179 (= T197), G180 (= G198), T182 (= T200), G222 (= G240), I223 (= I241), S266 (= S284), P293 (= P311), D294 (≠ S312)

4lmaA Crystal structure analysis of o-acetylserine sulfhydrylase cysk1 from microcystis aeruginosa 7806 (see paper)
59% identity, 95% coverage: 17:326/326 of query aligns to 2:311/318 of 4lmaA

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4lmaA

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active site: K46 (= K61), S269 (= S284)
binding pyridoxal-5'-phosphate: K46 (= K61), N77 (= N92), G181 (= G196), T182 (= T197), G183 (= G198), T185 (= T200), G225 (= G240), S269 (= S284), P296 (= P311)

3zeiA Structure of the mycobacterium tuberculosis o-acetylserine sulfhydrylase (oass) cysk1 in complex with a small molecule inhibitor (see paper)
63% identity, 92% coverage: 18:318/326 of query aligns to 3:300/300 of 3zeiA

query
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active site: K44 (= K61), S266 (= S284), P293 (= P311)
binding 3-[(Z)-[(5Z)-5-[[2-(2-hydroxy-2-oxoethyloxy)phenyl]methylidene]-3-methyl-4-oxidanylidene-1,3-thiazolidin-2-ylidene]amino]benzoic acid: T71 (= T89), S72 (= S90), I126 (≠ V144), Q144 (= Q162), F145 (= F163), K215 (≠ P233), G222 (= G240), A225 (= A243), F227 (= F245)
binding pyridoxal-5'-phosphate: K44 (= K61), N74 (= N92), V177 (= V195), G178 (= G196), T179 (= T197), G180 (= G198), T182 (= T200), G222 (= G240), S266 (= S284), P293 (= P311), D294 (≠ S312)

2q3cA 2.1 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) holoenzyme from mycobacterium tuberculosis in complex with the inhibitory peptide dfsi (see paper)
63% identity, 92% coverage: 18:318/326 of query aligns to 3:300/300 of 2q3cA

query
sites
2q3cA

I

I

Y

A

D

E

S

D

I

I

T

T

Q

Q

T

L

I

I

G

G

D

R

T

T

P

P

I

L

V

V

R

R

L

L

D

R

R

R

I

V

A

T

A

-

E

-

K

D

G

G

V

A

K

V

A

A

N

D

L

I

L

V

A

A

K

K

L

L

E

E

F

F

N

N

P

P

I

A

A

N

S

S

V

V

K
|
K

D

D

R

R

I

I

G

G

V

V

A

A

M

M

I

L

E

Q

S

A

L

A

E

E

K

Q

S

A

G

G

R

L

I

I

A

K

P

P

G

-

K

D

T

T

V

I

L

I

I

L

E

E

P

P

T
|
T

S
|
S

G
|
G

N

N

T
|
T

G

G

I

I

A

A

L

L

A

A

F

M

V

V

A

C

A

A

R

R

G

G

Y

Y

R

R

L

C

I

V

L

L

V

T

M

M

P

P

E

E

S

T

M

M

S

S

I

L

E

E

R

R

K

M

M

L

L

L

A

R

L

A

L

Y

G

G

A

A

E

E

L

L

V

I

L

L

T

T

P

P

A

G

A

A

L

D

G

G

M
|
M

K

S

G

G

A

A

V

I

A

A

K

K

A

A

L

E

E

E

L

L

A

A

A

K

E

T

N

D

P

Q

G

R

S

Y

V

F

I

V

P

P

Q

Q

Q
|
Q

F

F

E

E

N

N

P

P

A

A

N

N

P

P

E

A

I

I

H

H

R

R

L

V

T

T

A

A

E

E

I

V

W

W

N

R

D

D

T

T

A

D

G

G

A

K

I

V

D

D

Y

I

F

V

V

V

S

A

G

G

V

V

G

G

T

T

G

G

T

T

I

I

T

T

G

G

V

V

G

A

Q

Q

A

V

L

I

K

K

T

E

R

R

K

K

P

P

D

S

L

A

R

R

I

F

V

V

A

A

V

V

E

E

P

P

E

A

D

A

S

S

A

P

V

V

L

L

S

S

G

G

R

G

P

Q

P
x
K

G

G

P

P

H

H

K

P

I

I

Q

Q

G
|
G

I

I

G

G

A
|
A

G

G

F

F

V

V

P

P

S

P

I

V

L

L

D

D

R

Q

G

D

V

L

I

V

D

D

E

E

V

I

T

T

I

V

G

G

N

N

Q

E

T

D

A

A

F

L

E

N

T

V

A

A

R

R

L

L

A

A

R

R

L

E

E

E

G

G

V

L

P

L

G

V

G

G

I

I

S
|
S

S

S

G

G

A

A

V

T

A

V

A

A

L

L

E

Q

I

V

A

A

G

R

R

R

P

P

E

E

A

N

A

A

G

G

K

K

N

L

I

I

V

V

L

V

I

V

I

L

P
|
P

S

D

F

F

A

G

E

E

R

R

Y

Y

L

L

active site: K44 (= K61), S266 (= S284), P293 (= P311)
binding : T71 (= T89), S72 (= S90), G73 (= G91), T75 (= T93), M122 (= M140), Q144 (= Q162), K215 (≠ P233), G222 (= G240), A225 (= A243)

4lmbA Crystal structure analysis of o-acetylserine sulfhydrylase cysk2 complexed with cystine from microcystis aeruginosa 7806 (see paper)
59% identity, 94% coverage: 18:324/326 of query aligns to 3:309/310 of 4lmbA

query
sites
4lmbA

I

I

Y

A

D

R

S

D

I

I

T

T

Q

Q

T

L

I

V

G

G

D

R

T

T

P

P

I

L

V

V

R

Q

L

L

D

N

R

R

I

I

A

P

A

V

E

A

K

E

G

G

V

V

K

K

A

A

N

R

L

I

L

V

A

V

K

K

L

L

E

E

F

S

F

M

N

N

P

P

I

A

A

A

S

S

V

V

K
|
K

D

D

R

R

I

I

G

G

V

V

A

S

M

M

I

V

E

E

S

D

L

A

E

E

K

A

S

A

G

G

R

L

I

I

A

H

P

P

G

D

K

K

T

T

V

I

L

L

I

V

E

E

P

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

I

A

A

L

L

A

A

F

M

V

V

A

A

R

K

G

G

Y

Y

R

R

L

L

I

V

L

L

V

T

M

M

P

P

E

E

S

T

M
|
M

S

S

I

L

E

E

R

R

K

A

M

M

L

L

A

K

L

A

L

Y

G

G

A

A

E

Q

L

L

V

E

L

L

T

T

P

P

A

G

A

S

L

Q

G

G

M
|
M

K

E

G

G

A

A

V

I

A

T

K

R

A

A

L

E

E

E

L

I

A

V

A

E

E

N

N

T

P

P

G

N

S

A

V

Y

I

S

P

L

Q

Q

Q
|
Q

F
|
F

E

R

N

N

P

P

A

A

N

N

P

P

E

K

I

I

H

H

R

R

L

E

T

T

A

A

E

E

I

I

W

W

N

A

D

D

T

T

A

D

G

G

A

L

I

V

D

D

Y

I

F

V

V

I

S

G

G

G

V
|
V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

T

T

G

G

V

I

G

A

Q

E

A

T

L

I

K

K

T

P

R

R

K

R

P

P

D

Q

L

F

R

Q

I

A

V

I

A

A

V

V

E

E

P

P

E

S

D

N

S

S

A

P

V

V

L

L

S

S

G

G

R

G

P

Q

P

P

G

G

P

P

H

H

K

K

I

I

Q
|
Q

G
|
G

I
|
I

G

G

A
|
A

G

G

F

F

V

I

P

P

S

A

I

I

L

F

D

R

R

P

G

E

V

L

I

I

D

D

E

E

V

V

V

I

T

I

I

V

G

D

N

D

Q

T

T

E

A

A

F

F

E

A

T

Y

A

A

R

R

L

L

A

A

R

R

L

Q

E

E

G

G

V

L

P

L

G

S

G

G

I

I

S
|
S

S

A

G

G

A

A

V

L

A

W

A

A

L

I

E

Q

I

V

A

G

G

K

R

R

P

P

E

E

A

N

A

E

G

D

K

K

N

L

I

I

V

V

L

M

I

I

I

Q

P
|
P

S

S

F

F

A

G

E

E

R

R

Y

Y

L

L

S

S

T

T

A

A

L

L

F

F

E

K

active site: K46 (= K61), S269 (= S284)
binding cysteine: K46 (= K61), T74 (= T89), S75 (= S90), N77 (= N92), T78 (= T93), M101 (= M116), M125 (= M140), M125 (= M140), Q147 (= Q162), F148 (= F163), Q224 (= Q239), G225 (= G240), G225 (= G240), I226 (= I241), A228 (= A243)
binding pyridoxal-5'-phosphate: K46 (= K61), N77 (= N92), V180 (= V195), G181 (= G196), T182 (= T197), G183 (= G198), T185 (= T200), G225 (= G240), S269 (= S284), P296 (= P311)

5xoqA Crystal structure of o-acetylserine sulfhydrylase with bound transcription factor peptide inhibitor from planctomyces limnophilus
61% identity, 95% coverage: 18:326/326 of query aligns to 4:309/310 of 5xoqA

query
sites
5xoqA

I

I

Y

F

D

K

S

D

I

N

T

S

Q

E

T

S

I

I

G

G

D

R

T

T

P

P

I

L

V

V

R

Q

L

I

D

N

R

R

I

L

A

T

A

A

E

-

K

-

G

G

V

L

K

S

A

S

N

R

L

V

L

L

A

A

K

K

L

I

E

E

F

G

F

R

N

N

P

P

I

A

A

Y

S

S

V

V

K

K

D

C

R

R

I

I

G

G

V

A

A

A

M

M

I

I

E

W

S

D

L

A

E

E

K

Q

S

S

G

G

R

K

I

L

A

K

P

P

G

G

K

M

T

H

V

V

L

-

I

V

E

E

P

P

T
|
T

S
|
S

G
|
G

N

N

T
|
T

G

G

I

I

A

A

L

L

A

A

F

F

V

V

A

C

A

A

R

R

G

G

Y

Y

R

K

L

L

I

T

L

L

V

T

M

M

P

P

E

E

S

T

M

M

S

S

I

I

E

E

R

R

K

M

M

M

L

L

A

K

L

S

L

F

G

G

A

A

E

D

L

L

V

V

L

L

T

T

P

P

A

G

A

A

L

D

G

G

M
|
M

K

K

G

G

A

A

V

I

A

S

K

K

A

A

L

E

E

E

L

L

A

A

E

Q

N

-

P

P

G

G

S

W

V

F

I

I

P

P

Q

Q

Q
|
Q

F

F

E

K

N

N

P

P

A

A

N

N

P

P

E

A

I

I

H

H

R

V

L

K

T

T

A

G

E

P

E

E

I

I

W

W

N

N

D

D

T

T

A

E

G

G

A

Q

I

V

D

D

Y

V

F

F

V

V

S

A

G

G

V

V

G

G

T

T

G

G

T

T

I

I

T

T

G

G

V

V

G

A

Q

R

A

F

L

L

K

K

T

H

-

E

R

K

K

K

P

H

D

P

L

V

R

H

I

V

V

V

A

A

V

V

E

E

P

P

E

A

D

A

S

S

A

P

V

V

L

L

S

A

G

G

R

G

P

P

P

A

G

G

P
x
R

H
|
H

K

K

I

I

Q
|
Q

G
|
G

I

I

G

G

A
|
A

G

G

F

F

V

V

P

P

S

D

I

T

L

F

D

D

R

R

G

S

V

V

I

V

D

D

E

E

V

I

V

L

T

S

I

V

G

T

N

D

Q

D

T

E

A

A

F

I

E

E

T

T

A

A

R

R

R

K

L

L

A

A

R

M

L

E

E

E

G

G

V

I

P

S

G

C

G

G

I

I

S

S

S

C

G

G

A

A

V

M

A

A

A

G

A

A

L

L

E

K

I

V

A

A

G

A

R

R

P

P

E

E

A

F

A

A

G

G

K

K

N

T

I

I

V

V

L

T

I

V

I

L

P

P

S

D

F

A

A

G

E

E

R

R

Y

Y

L

L

S

S

T

T

A

A

L

L

F

F

E

E

G

N

L

L

binding : T72 (= T89), S73 (= S90), G74 (= G91), T76 (= T93), M123 (= M140), Q144 (= Q162), R218 (≠ P235), H219 (= H236), Q222 (= Q239), G223 (= G240), A226 (= A243)

4aecA Crystal structure of the arabidopsis thaliana o-acetyl-serine-(thiol)- lyasE C (see paper)
58% identity, 95% coverage: 17:326/326 of query aligns to 12:319/323 of 4aecA

query
sites
4aecA

R

N

I

I

Y

A

D

D

S

N

I

V

T

S

Q

Q

T

L

I

I

G

G

D

K

T

T

P

P

I

M

V

V

R

Y

L

L

D

N

R

S

I

I

A

A

A

-

E

-

K

K

G

G

V

C

K

V

A

A

N

N

L

I

L

A

A

A

K

K

L

L

E

E

F

I

F

M

N

E

P

P

I

C

A

C

S

S

V

V

K
|
K

D

D

R

R

I

I

G

G

V

Y

A

S

M

M

I

V

E

T

S

D

L

A

E

E

K

Q

S

K

G

G

R

F

I

I

A

S

P

P

G

G

K

K

T

S

V

V

L

L

I

V

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

G

L

L

A

A

F

F

V

I

A

A

A

S

R

R

G

G

Y

Y

R

R

L

L

I

I

L

L

V

T

M

M

P

P

E

A

S

S

M

M

S

S

I

M

E

E

R

R

K

V

M

L

L

L

A

K

L

A

L

F

G

G

A

A

E

E

L

L

V

V

L

L

T

T

P

D

A

P

A

A

L

K

G

G

M

M

K

T

G

G

A

A

V

V

A

Q

K

K

A

A

L

E

E

E

L

I

A

L

A

K

E

N

N

T

P

P

G

D

S

A

V

Y

I

M

P

L

Q

Q

F

F

E

D

N

N

P

P

A

A

N

N

P

P

E

K

I

I

H

H

R

Y

L

E

T

T

A

G

E

P

E

E

I

I

W

W

N

D

D

D

T

T

A

K

G

G

A

K

I

V

D

D

Y

I

F

F

V

V

S

A

G

G

V
x
I

G
|
G

T
|
T

G
|
G

T
|
T

I

I

T

T

G

G

V

V

G

G

Q

R

A

F

L

I

K

K

T

E

R

K

K

N

P

P

D

K

L

T

R

Q

I

V

V

I

A

G

V

V

E

E

P

P

E

T

D

E

S

S

A

D

V

I

L

L

S

S

G

G

R

G

P

K

P

P

G

G

P

P

H

H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

S

K

I

N

L

L

D

D

R

Q

G

K

V

I

I

M

D

D

E

E

V

V

V

I

T

A

I

I

G

S

N

S

Q

E

T

E

A

A

F

I

E

E

T

T

A

A

R

K

R

Q

L

L

A

A

R

L

L

K

E

E

G

G

V

L

P

M

G

V

G

G

I

I

S
|
S

S

S

G

G

A

A

V

A

A

A

L

I

E

K

I

V

A

A

G

K

R

R

P

P

E

E

A

N

A

A

G

G

K

K

N

L

I

I

V

A

L

V

I

V

I

F

P
|
P

S

S

F

F

A

G

E

E

R

R

Y

Y

L

L

S

S

T

T

A

P

L

L

F

F

E

Q

G

S

L

I

active site: K54 (= K61), S277 (= S284)
binding pyridoxal-5'-phosphate: K54 (= K61), N85 (= N92), I188 (≠ V195), G189 (= G196), T190 (= T197), G191 (= G198), G192 (= G199), T193 (= T200), G233 (= G240), S277 (= S284), P304 (= P311)

P47999 Cysteine synthase, chloroplastic/chromoplastic; At.OAS.7-4; Beta-substituted Ala synthase 2;1; ARAth-Bsas2;1; CSase B; AtCS-B; CS-B; O-acetylserine (thiol)-lyase; O-acetylserine sulfhydrylase; OAS-TL B; cpACS1; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
58% identity, 95% coverage: 18:326/326 of query aligns to 75:381/392 of P47999

query
sites
P47999

I

I

Y

A

D

D

S

N

I

A

T

A

Q

Q

T

L

I

I

G

G

D

K

T

T

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P

I

M

V

V

R

Y

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L

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N

R

N

I

V

A

V

A

-

E

-

K

K

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G

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C

K

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A

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S

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A

A

K

K

L

L

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E

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I

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E

P

P

I

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V

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K

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D

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R

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Y

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M

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A

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E

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P

G

G

K

K

T

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V

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L

L

I

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E

P

S

T

T

S

S

G

G

N

N

T

T

G

G

I

I

A

G

L

L

A

A

F

F

V

I

A

A

A

S

R

K

G

G

Y

Y

R

K

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I

I

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T

M

M

P

P

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M

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M

L

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R

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A

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E

A

P

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M

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G

G

A

A

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I

A

Q

K

K

A

A

L

E

E

E

L

I

A

L

A

K

E

K

N

T

P

P

G

N

S

S

V

Y

I

M

P

L

Q

Q

F

F

E

D

N

N

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P

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A

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N

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K

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H

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E

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L

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H

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A

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A

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L

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G

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G

A

A

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A

A

A

L

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E

Q

I

V

A

A

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R

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P

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N

A

A

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K

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L

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A

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L

I

Sites not aligning to the query:

61 modified: N-acetylalanine

7n2tA O-acetylserine sulfhydrylase from citrullus vulgaris in the internal aldimine state, with citrate bound (see paper)
59% identity, 95% coverage: 18:326/326 of query aligns to 3:309/309 of 7n2tA

query
sites
7n2tA

I

I

Y

A

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A

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A

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K

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S

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N

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M

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M

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A

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K

A

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A

K

A

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K

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T

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P

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Y

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Q

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F

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A

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L

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A

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K

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L

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x
R

I

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R
|
R

P

P

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A

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binding phosphate ion: R277 (≠ E294), R281 (= R298)

2isqA Crystal structure of o-acetylserine sulfhydrylase from arabidopsis thaliana in complex with c-terminal peptide from arabidopsis serine acetyltransferase (see paper)
58% identity, 94% coverage: 17:324/326 of query aligns to 2:307/320 of 2isqA

query
sites
2isqA

R

R

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K

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N

I

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A

A

A

-

E

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L

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A

A

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M

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S

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T

G

G

I

V

A

G

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L

A

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A

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K

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Y

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P

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K

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G

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A

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I

A

A

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K

A

A

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E

E

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N

T

P

P

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Q

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F

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E

N

N

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A

A

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N

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H

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K

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G

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A

A

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L

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L

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G

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G

A

A

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A

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L

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K

I

L

A

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A

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G

K

K

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V

L

A

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|
P

S

S

F

F

A

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E

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R

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Y

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L

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F

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D

active site: K44 (= K61), S267 (= S284)
binding pyridoxal-5'-phosphate: K44 (= K61), N75 (= N92), G177 (= G194), G179 (= G196), T180 (= T197), G181 (= G198), T183 (= T200), G223 (= G240), S267 (= S284), P294 (= P311)
binding : T72 (= T89), S73 (= S90), G74 (= G91), T76 (= T93), G122 (= G139), M123 (= M140), K124 (= K141), G217 (= G234), P218 (= P235), H219 (= H236), Q222 (= Q239), G223 (= G240)

P47998 Cysteine synthase 1; At.OAS.5-8; Beta-substituted Ala synthase 1;1; ARAth-Bsas1;1; CSase A; AtCS-A; Cys-3A; O-acetylserine (thiol)-lyase 1; OAS-TL A; O-acetylserine sulfhydrylase; Protein ONSET OF LEAF DEATH 3; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
58% identity, 94% coverage: 17:324/326 of query aligns to 4:309/322 of P47998

query
sites
P47998

R

R

I

I

Y

A

D

K

S

D

I

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T

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Q

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L

I

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N

T

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P

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L

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V

R

Y

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L

D

N

R

N

I

V

A

A

A

-

E

-

K

E

G

G

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C

K

V

A

G

N

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L

A

A

A

K

K

L

L

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E

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P

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K

D

D

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M

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K

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V

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S
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S

G

G

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|
N

T
|
T

G

G

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V

A

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L

A

A

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A

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M

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A

L

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T

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A

P

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M

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K

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G

A

A

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I

A

A

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A

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E

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L

I

A

L

A

A

E

K

N

T

P

P

G

N

S

G

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Y

I

M

P

L

Q

Q

Q
|
Q

F

F

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E

N

N

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P

A

A

N

N

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P

E

K

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I

H
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H

R

Y

L

E

T

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A
x
G

E

P

E

E

I

I

W

W

N

K

D

G

T

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A

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A

K

I

I

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D

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G

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F

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S

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T

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I

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K

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K

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S

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G

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x
K

P

P

G

G

P

P

H
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H

K
|
K

I

I

Q

Q

G

G

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I

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A

A

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G

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I

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P

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S

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K

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E

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L

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S

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G

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A

A

A

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E

K

I

L

A

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R

P

P

E

E

A

N

A

A

G

G

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K

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V

L

A

I

I

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P

S

S

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F

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D

K46 (= K61) modified: N6-(pyridoxal phosphate)lysine; mutation to A: No cysteine synthase activity.
T74 (= T89) mutation to A: Strong reduction of cysteine synthase activity.; mutation to S: Reduction of cysteine synthase activity.
S75 (= S90) mutation S->A,N,T: Strong reduction of cysteine synthase activity.
N77 (= N92) binding pyridoxal 5'-phosphate; mutation to A: Reduction of cysteine synthase activity.; mutation to D: Strong reduction of cysteine synthase activity.
T78 (= T93) mutation T->A,S: Reduction of cysteine synthase activity.
Q147 (= Q162) mutation Q->A,E: Strong reduction of cysteine synthase activity.
H157 (= H172) mutation H->Q,N: Slight reduction of cysteine synthase activity.
G162 (≠ A177) mutation to E: In old3-1; displays a early leaf death phenotype. Abolishes cysteine synthase activity.
GTGGT 181:185 (= GTGGT 196:200) binding pyridoxal 5'-phosphate
T182 (= T197) mutation T->A,S: Slight reduction of cysteine synthase activity.
T185 (= T200) mutation T->A,S: Strong reduction of cysteine synthase activity.
K217 (≠ P232) mutation to A: Impaired interaction with SAT1.
H221 (= H236) mutation to A: Impaired interaction with SAT1.
K222 (= K237) mutation to A: Impaired interaction with SAT1.
S269 (= S284) binding pyridoxal 5'-phosphate; mutation to A: Strong reduction of cysteine synthase activity.; mutation to T: Reduction of cysteine synthase activity.

1z7yA Crystal structure of the arabidopsis thaliana o-acetylserine sulfhydrylase k46a mutant (see paper)
58% identity, 94% coverage: 17:324/326 of query aligns to 2:307/320 of 1z7yA

query
sites
1z7yA

R

R

I

I

Y

A

D

K

S

D

I

V

T

T

Q

E

T

L

I

I

G

G

D

N

T

T

P

P

I

L

V

V

R

Y

L

L

D

N

R

N

I

V

A

A

A

-

E

-

K

E

G

G

V

C

K

V

A

G

N

R

L

V

L

A

A

A

K

K

L

L

E

E

F

M

N

E

P

P

I

C

A

S

S

S

V

V

K
x
A

D

D

R

R

I

I

G

G

V

F

A

S

M

M

I

I

E

S

S

D

L

A

E

E

K

K

S

K

G

G

R

L

I

I

A

K

P

P

G

G

K

E

T

S

V

V

L

L

I

I

E

E

P

P

T

T

S

S

G
|
G

N
|
N

T
|
T

G

G

I

V

A

G

L

L

A

A

F

F

V

T

A

A

R

K

G

G

Y

Y

R

K

L

L

I

I

L

I

V

T

M

M

P

P

E

A

S

S

M

M

S

S

I

T

E

E

R

R

K

I

M

I

L

L

A

L

L

A

L

F

G

G

A

V

E

E

L

L

V

V

L

L

T

T

P

D

A

P

A

A

L

K

G

G

M

M

K

K

G

G

A

A

V

I

A

A

K

K

A

A

L

E

E

E

L

I

A

L

A

A

E

K

N

T

P

P

G

N

S

G

V

Y

I

M

P

L

Q

Q

Q
|
Q

F

F

E

E

N

N

P

P

A

A

N

N

P

P

E

K

I

I

H

H

R

Y

L

E

T

T

A

G

E

P

E

E

I

I

W

W

N

K

D

G

T

T

A

G

G

G

A

K

I

I

D

D

Y

G

F

F

V

V

S

S

G

G

V
x
I

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

T

T

G

G

V

A

G

G

Q

K

A

Y

L

L

K

K

T

E

R

Q

K

N

P

A

D

N

L

V

R

K

I

L

V

Y

A

G

V

V

E

E

P

P

E

V

D

E

S

S

A

A

V

I

L

L

S

S

G

G

R

G

P

K

P

P

G

G

P

P

H

H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

S

S

I

V

L

L

D

N

R

V

G

D

V

L

I

I

D

D

E

E

V

V

T

Q

I

V

G

S

N

S

Q

D

T

E

A

S

F

I

E

D

T

M

A

A

R

R

R

Q

L

L

A

A

R

L

L

K

E

E

G

G

V

L

P

L

G

V

G

G

I

I

S
|
S

S

S

G

G

A

A

V

A

A

A

L

I

E

K

I

L

A

A

G

Q

R

R

P

P

E

E

A

N

A

A

G

G

K

K

N

L

I

F

V

V

L

A

I

I

I

F

P
|
P

S
|
S

F

F

A

G

E

E

R

R

Y

Y

L

L

S

S

T

T

A

V

L

L

F

F

E

D

active site: A44 (≠ K61), S267 (= S284)
binding n-[(3-hydroxy-2-methyl-5-{[(trihydroxyphosphoranyl)oxy]methyl}pyridin-4-yl)methylene]methionine: G74 (= G91), N75 (= N92), T76 (= T93), Q145 (= Q162), I178 (≠ V195), G179 (= G196), T180 (= T197), G181 (= G198), T183 (= T200), G223 (= G240), S267 (= S284), P294 (= P311), S295 (= S312)

8b9wA Cysteine synthase from trypanosoma theileri with plp bound (see paper)
55% identity, 93% coverage: 21:323/326 of query aligns to 12:311/329 of 8b9wA

query
sites
8b9wA

S

S

I

V

T

D

Q

A

T

L

I

I

G

G

D

N

T

T

P

P

I

A

V

V

R

Y

L

L

D

R

R

N

I

M

A

-

E

-

K

N

G

K

V

T

K

A

A

A

N

T

L

I

L

V

A

L

K

K

L

L

E

E

F

S

F

E

N

N

P

P

I

M

A

A

S

S

V

V

K
|
K

D

D

R

R

I

L

G

A

V

Y

A

A

M

I

I

Y

E

D

S

K

L

A

E

E

K

K

S

E

G

G

R

K

I

I

A

K

P

R

G

G

K

K

T

S

V

V

L

I

I

V

E

E

P

A

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

F

L

V

L

A

G

A

A

A

V

R

R

G

G

Y

Y

R

K

L

V

I

I

L

I

V

T

M

M

P

P

E

D

S

S

M

M

S

S

I

M

E

E

R

R

K

S

M

L

L

L

A

R

L

I

L

F

G

G

A

A

E

E

L

L

V

I

L

L

T

T

P

P

A

A

A

S

L

L

G

G

M

M

K

K

G

G

A

A

V

V

A

A

K

M

A

A

L

N

E

R

L

I

A

V

A

S

E

N

N

N

P

P

G

D

S

A

V

V

I

L

P

A

Q

D

Q

Q

F

F

E

C

N

T

P

K

A

Y

N

N

P

A

E

Q

I

I

H

H

R

E

L

E

T

T

A

G

E

P

E

E

I

I

W

W

N

R

D

Q

T

T

A

K

G

G

A

H

I

V

D

D

Y

C

F

F

V

V

S

A

G

G

V

V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

L

T

T

G

G

V

V

G

A

Q

R

A

F

L

L

K

K

T

S

R

V

K

G

P

C

D

N

L

A

R

N

I

I

V

V

A

A

V

V

E

E

P

P

E

Q

D

E

S

S

A

P

V

V

L

L

S

S

G

G

R

G

P

R

P

P

G

G

P

P

H

H

K

R

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

V

V

P

P

S

E

I

V

L

L

D

D

R

R

G

E

V

L

I

V

D

D

E

E

V

I

T

Q

I

V

G

S

N

S

Q

D

T

K

A

A

F

I

E

E

T

T

A

A

R

Q

R

K

L

L

A

S

R

R

L

M

E

D

G

G

V

I

P

F

G

C

G

G

I

F

S
|
S

S

A

G

G

A

A

A

N

V

V

A

F

A

A

L

L

E

K

I

I

A

A

G

E

R

R

P

P

E

E

A

M

A

A

G

G

K

K

N

T

I

I

V

V

L

T

I

V

I

I

P
|
P

S

S

F

F

A

G

E

E

R

R

Y

Y

L

L

S

S

T

T

A

A

L

L

F

Y

binding pyridoxal-5'-phosphate: K49 (= K61), N80 (= N92), G184 (= G196), T185 (= T197), G186 (= G198), T188 (= T200), G228 (= G240), S272 (= S284), P299 (= P311)

3t4pA Crystal structure of o-acetyl serine sulfhydrylase from leishmania donovani in complex with designed tetrapeptide (see paper)
54% identity, 95% coverage: 18:326/326 of query aligns to 10:315/319 of 3t4pA

query
sites
3t4pA

I

V

Y

A

D

Q

S

S

I

I

T

D

Q

Q

T

L

I

I

G

G

D

Q

T

T

P

P

I

A

V

L

R

Y

L

L

D

N

R

K

I

L

A

-

E

-

K

N

G

N

V

T

K

K

A

A

N

K

L

V

A

L

K

K

L

M

E

E

F

C

F

E

N

N

P

P

I

M

A

A

S

S

V

V

K
|
K

D

D

R

R

I

L

G

G

V

F

A

A

M

I

I

Y

E

D

S

K

L

A

E

E

K

K

S

E

G

G

R

K

I

L

A

I

P

P

G

G