SitesBLAST

Q8NLB7 Gentisate transporter from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / BCRC 11384 / CCUG 27702 / LMG 3730 / NBRC 12168 / NCIMB 10025 / NRRL B-2784 / 534) (see paper)
25% identity, 31% coverage: 239:436/640 of query aligns to 52:230/444 of Q8NLB7

query
sites
Q8NLB7

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D54 (≠ E241) mutation to A: Loss of transport activity.; mutation to E: Retains 50% of its transport activity.
D57 (= D244) mutation to A: Loss of transport activity.; mutation to E: Retains 50% of its transport activity.
R103 (= R296) mutation to A: Loss of transport activity.

Sites not aligning to the query:

309 W→V: Loss of transport activity.
312 D→A: Loss of transport activity.
313 R→A: Loss of transport activity.
317 mutation I->H,Y: Loss of transport activity.
386 R→A: Loss of transport activity.

P0AGF4 D-xylose-proton symporter; D-xylose transporter from Escherichia coli (strain K12) (see paper)
28% identity, 50% coverage: 277:598/640 of query aligns to 62:429/491 of P0AGF4

query
sites
P0AGF4

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G83 (= G298) mutation to A: Abolishes xylose transport.
R133 (= R338) mutation R->C,H,L: Abolishes xylose transport.
E153 (= E358) mutation to A: Abolishes xylose transport.
R160 (= R365) mutation to A: Abolishes xylose transport.
Q168 (≠ P373) binding beta-D-xylose; mutation to A: Abolishes xylose transport.
Q288 (= Q481) mutation to A: Abolishes xylose transport.
QQ 288:289 (= QQ 481:482) binding beta-D-xylose
Q289 (= Q482) mutation to A: Strongly decreases xylose transport.
N294 (vs. gap) binding beta-D-xylose; mutation to A: Abolishes xylose transport.
Y298 (vs. gap) mutation to A: Abolishes xylose transport.
N325 (vs. gap) mutation to A: No effect on xylose transport.
G340 (= G515) mutation to A: Abolishes xylose transport.
R341 (= R516) mutation R->A,W: Abolishes xylose transport.
W392 (≠ P564) binding beta-D-xylose; mutation to A: Abolishes xylose transport.
E397 (= E569) mutation to A: Abolishes xylose transport.
R404 (= R576) mutation to A: Strongly decreases xylose transport.
Q415 (vs. gap) binding beta-D-xylose
W416 (vs. gap) mutation to A: Strongly decreases xylose transport.

Sites not aligning to the query:

24 F→A: Decreases xylose transport.

4gc0A The structure of the mfs (major facilitator superfamily) proton:xylose symporter xyle bound to 6-bromo-6-deoxy-d-glucose (see paper)
28% identity, 50% coverage: 277:598/640 of query aligns to 58:425/475 of 4gc0A

query
sites
4gc0A

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binding 6-bromo-6-deoxy-beta-D-glucopyranose: Q164 (≠ P373), Q284 (= Q481), N290 (vs. gap), L293 (vs. gap), W388 (≠ P564)

4gbzA The structure of the mfs (major facilitator superfamily) proton:xylose symporter xyle bound to d-glucose (see paper)
28% identity, 50% coverage: 277:598/640 of query aligns to 58:425/475 of 4gbzA

query
sites
4gbzA

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-

V

-

L

-

Y

-

A

-

P

-

E

-

V

-

F

-

K

-

T

-

L

-

G

A

A

S

S

S

T

T

D

A

I

L

A

T

L

L

L

N

Q

S

T

I

I

A

I

I

V

F

G

A

V

M

I

-

N

L

L

I

T

F

F

T

T

P

V

L

L

A

A

G

I

A

M

L

T

S

V

D

D

R

K

I

F

G

G

R

R

K

K

P

P

V

L

L

Q

I

I

A

I

G

G

A

A

A

L

G

G

M

M

A

A

-

I

-

G

L

M

F

F

A

S

W

L

P

G

L

T

L

A

W

F

I

Y

I

T

D

Q

T

A

P

P

H

G

L

I

P

V

A

A

I

L

L

L

A

S

G

M

L

L

-

F

-

Y

-

V

G

A

A

A

F

F

A

A

L

-

L

M

S

S

C

W

N

G

G

P

A

V

G

C

P
x
W

A

V

F

L

L

L

V

S

E

E

A

I

F

F

P

P

K

N

H

A

V

I

R

R

C

G

S

K

G

A

L

L

S

A

I

I

A

A

Y

V

N

A

-

A

-

Q

-

W

L

L

A

A

Q

N

S

Y

I

F

F

V

G

S

G

W

T

T

V

F

P

P

M

M

V

M

binding beta-D-glucopyranose: Q164 (≠ P373), Q284 (= Q481), N290 (vs. gap), W388 (≠ P564)

4gbyA The structure of the mfs (major facilitator superfamily) proton:xylose symporter xyle bound to d-xylose (see paper)
28% identity, 50% coverage: 277:598/640 of query aligns to 58:425/475 of 4gbyA

query
sites
4gbyA

A

A

A

S

G

A

F

L

L

I

A

G

R

C

P

I

L

I

G

G

L

A

L

L

F

G

G

G

H

Y

I

C

G

S

D

N

R

R

L

F

G

G

R

R

A

D

A

S

V

L

V

K

S

I

S

A

V

A

V

V

L

L

M

F

I

F

I

I

-

S

-

G

-

V

-

G

-

S

-

A

-

W

P

P

T

E

L

L

-

G

F

F

M

T

A

S

L

I

L

N

P

P

T

D

Y

N

A

T

Q

-

I

-

G

-

V

-

A

-

A

V

P

P

I

V

L

Y

L

L

-

A

-

G

-

Y

-

V

-

P

-

E

-

F

V

V

L

I

L

Y

R

R

L

I

A

I

Q

G

G

G

L

I

A

G

V

V

G

G

G

L

E

A

Y

S

T

M

T

L

S

S

M

P

V

M

L

Y

L

I

V

A

E

E

E

L

A

A

Q

P

P

A

S

H

R

I

R

R

G

G

F

K

V

L

G

V

S

S

F

F

A

N

P
x
Q

F

F

G

A

A

I

L

I

G

F

G

G

L

Q

L

L

G

V

S

Y

A

C

V

V

G

N

A

-

L

-

L

Y

A

F

I

I

L

A

P

R

E

S

R

G

D

D

A

A

S

S

-

W

-

L

-

N

T

T

W

D

G

G

W

W

R

R

L

Y

A

M

F

F

F

A

S

S

G

E

L

C

L

I

I

P

G

A

L

L

V

L

V

F

F

L

L

M

I

L

R

L

R

Y

R

T

L

V

P

P

P

E

D

S

K

P

K

R

I

W

L

L

-

M

-

S

-

R

-

G

-

K

-

Q

-

E

E

Q

A

A

E

E

Q

G

A

I

R

L

K

R

S

K

P

I

L

M

G

G

E

N

A

T

F

L

R

A

T

T

Q

Q

W

-

R

-

T

-

I

A

L

V

K

Q

V

E

L

I

G

K

F

H

T

S

L

L

G

D

H

H

G

G

-

R

-

K

-

T

-

G

-

R

-

L

-

M

-

F

-

G

V

V

G

G

F

V

Y

I

L

V

C

I

F

G

V

V

Y

M

L

L

S

S

T

I

W

F

L

-

K

-

E

-

Q
|
Q

Q

Q

H

F

V

V

-

G

-

I

-
x
N

-

V

-

L

-

Y

-

A

-

P

-

E

-

V

-

F

-

K

-

T

-

L

-

G

A

A

S

S

S

T

T

D

A

I

L

A

T

L

L

L

N

Q

S

T

I

I

A

I

I

V

F

G

A

V

M

I

-

N

L

L

I

T

F

F

T

T

P

V

L

L

A

A

G

I

A

M

L

T

S

V

D

D

R

K

I

F

G

G

R

R

K

K

P

P

V

L

L

Q

I

I

A

I

G

G

A

A

A

L

G

G

M

M

A

A

-

I

-

G

L

M

F

F

A

S

W

L

P

G

L

T

L

A

W

F

I

Y

I

T

D

Q

T

A

P

P

H

G

L

I

P

V

A

A

I

L

L

L

A

S

G

M

L

L

-

F

-

Y

-

V

G

A

A

A

F

F

A

A

L

-

L

M

S

S

C

W

N

G

G

P

A

V

G

C

P
x
W

A

V

F

L

L

L

V

S

E

E

A

I

F

F

P

P

K

N

H

A

V

I

R

R

C

G

S

K

G

A

L

L

S

A

I

I

A

A

Y

V

N

A

-

A

-

Q

-

W

L

L

A

A

Q

N

S

Y

I

F

F

V

G

S

G

W

T

T

V

F

P

P

M

M

V

M

binding beta-D-xylopyranose: Q164 (≠ P373), Q284 (= Q481), N290 (vs. gap), W388 (≠ P564)

Q09852 Putative inorganic phosphate transporter C23D3.12 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
30% identity, 20% coverage: 275:401/640 of query aligns to 93:223/559 of Q09852

query
sites
Q09852

V

V

F

N

A

A

G

A

F

N

L

I

A

G

R

N

P

V

L

F

G

G

G

Q

L

I

L

L

F

F

G

G

H

F

I

M

G

G

D

D

R

F

L

F

G

G

R

R

R

K

A

F

A

V

V

Y

V

G

S

K

S

E

V

M

V

I

L

V

M

V

I

I

P

A

T

T

L

V

F

L

M

V

A

I

L

A

L

L

P

P

T

K

Y

S

A

I

Q

P

I

T

G

P

V

L

A

G

A

K

P

M

I

M

L

W

L

I

V

F

L

A

L

W

R

R

L

W

A

L

Q

L

G

G

L

L

A

G

V

I

G

G

E

D

Y

Y

T

P

T

M

S

S

M

A

V

T

L

I

L

T

V

S

E

E

E

R

A

S

Q

L

P

L

S

S

R

R

G

G

-

T

-

L

-

S

F

I

V

V

G

F

S

S

F

F

A

Q

P

G

F

F

G

G

A

T

L

L

G

A

G

G

L

A

L

I

L

V

G

T

S

I

A

I

V

L

G

L

A

A

A

C

L

F

L

E

A

K

I

P

L

L

P

N

E

Q

R

R

D

G

A

E

S

Y

T

T

Sites not aligning to the query:

297 modified: Phosphoserine
299 modified: Phosphoserine

O42885 Putative inorganic phosphate transporter C8E4.01c from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
27% identity, 27% coverage: 275:444/640 of query aligns to 98:273/572 of O42885

query
sites
O42885

V

V

F

N

A

A

A

S

G

A

F

N

L

I

A

G

R

N

P

I

L

F

G

G

G

Q

L

L

F

F

G

G

H

F

I

M

G

G

D

D

R

F

L

F

G

G

R

R

R

K

A

F

A

V

V

Y

V

G

S

K

S

E

V

M

V

V

L

I

M

V

I

I

P

A

T

T

L

V

F

L

M

V

A

I

L

A

L

M

P

P

T

K

Y

S

A

I

Q

H

I

S

G

P

V

L

A

S

A

K

P

M

I

M

L

W

L

V

V

F

L

C

L

W

R

R

L

W

A

L

Q

L

G

G

L

V

A

G

V

I

G

G

E

D

Y

Y

T

P

T

M

S

S

M

A

V

A

L

I

L

T

V

S

E

E

E

R

A

S

Q

K

P

I

S

K

R

R

G

G

-

T

-

L

-

I

-

S

F

L

V

I

G

F

S

A

F

F

A

Q

P

G

F

F

G

G

A

T

L

L

G

A

G

G

L

A

L

I

L

-

G

-

S

-

A

-

V

V

G

T

A

I

L

L

A

G

I

C

L

F

P

E

E

H

-

P

-

L

-

N

R

R

D

E

A

G

S

H

T

Y

W

H

G

K

-

L

-

E

-

G

-

V

W

W

R

R

L

L

A

Q

F

F

F

-

S

-

G

G

L

L

-

A

L

L

I

V

G

P

L

A

V

I

V

G

F

V

L

L

I

I

R

P

R

R

R

L

L

I

P

M

P

K

D

E

K

S

K

K

I

S

L

Y

E

E

A

N

E

S

Q

K

A

A

R

L

K

N

S

S

P

A

L

E

G

G

E

K

Sites not aligning to the query:

12 modified: Phosphoserine
14 modified: Phosphoserine
292 modified: Phosphoserine
296 modified: Phosphoserine

Q9H4A6 Golgi phosphoprotein 3; Coat protein GPP34; Mitochondrial DNA absence factor; MIDAS from Homo sapiens (Human) (see 3 papers)
26% identity, 31% coverage: 10:206/640 of query aligns to 55:275/298 of Q9H4A6

query
sites
Q9H4A6

S

S

R

K

E

E

T

T

L

-

P

-

Q

R

I

L

S

T

L

L

L

M

E

E

F

V

L

L

A

G

L

L

E

K

D

D

G

R

G

E

G

G

E

-

F

Y

D

T

N

S

V

F

P
x
W

E

N

I

D

Y
x
C

L

I

S

S

C

S

G

G

V

L

A
x
R

G

G

A

C

T

M

L

L

M

I

D

E

L

L

A

A

L

L

R

R

D

G

R

R

I

L

D

Q

S

L

D

E

L

A

E
x
C

G

G

-

M

-

R

-

K

-

S

-

L

-

T

-

R

-

K

V

V

F

I

A

C

V

K

N

S

S

D

A

A

P

P

T

T

G

G

D

D

A

V

I

L

L

L

D

D

R

E

V

A

L

L

A

K

E

H

I

V

V

K

A

E

E

T

P

Q

K

P

R

P

L

E

S

T

P

V

Q

Q

E

N

W

W

I

I

S

E

-

L

-

S

-

G

-

E

-

T

-

W

-

N

-

P

-

L

R

K

L

L

S

H

P

Y

Q

Q

A

L

P
x
R

A

N

L

V

H
x
R

K

E

A

R

A

L

L

A

S

K

E

N

L

L

C

V

A

E

R

K

G

G

V

V

L

L

R

T

Q

T

S

E

D

K

H

Q

A

N

F

F

L

L

W

-

V

L

L

F

K

D

E

M

R

T

Y

H

P

P

I

L

V

T

E

N

G

N

-

N

-

I

Q

K

E

Q

R

R

-

L

-

I

P

K

E

K

A

V

K

Q

R

E

R

A

I

V

L

L

A

D

L

K

L

W

Y

V

N

N

D

D

D

-

L

-

P

P

S

H

P

R

A

M

D

D

-

R

-

L

V

L

A

A

L

L

T

I

A

Y

L

L

A

A

N

H

A

A

C

S

F

D

L

V

F

L

E

E

R

N

I

A

L

F

T

A

P

P

-

L

-

D

K

E

E

Q

L

Y

K

D

R

L

V

A

K

T

P

K

R

R

I

V

E

R

Q

Q

I

L

A

L

R

D

L

L

D

D

W81 (≠ P39) mutation to A: Abolishes phosphoinositide binding and localization to the Golgi apparatus; when associated with A-90.
C84 (≠ Y42) modified: Disulfide link with 108
R90 (≠ A48) mutation to A: Abolishes phosphoinositide binding and localization to the Golgi apparatus; when associated with A-81.; mutation to L: Loss of function in vesicle budding, abolishes phosphoinositide binding and localization to the Golgi apparatus.
C108 (≠ E66) modified: Disulfide link with 84
R171 (≠ P109) mutation R->A,L: Abolishes phosphoinositide binding and localization to the Golgi apparatus; when associated with A-174.
R174 (≠ H112) mutation to A: Abolishes phosphoinositide binding and localization to the Golgi apparatus; when associated with A-171 or L-171.

Sites not aligning to the query:

7 R→A: Altered binding to coatomer.
14:15 RR→AA: Loss of binding to coatomer.

3kn1A Crystal structure of golgi phosphoprotein 3 n-term truncation variant (see paper)
25% identity, 30% coverage: 16:206/640 of query aligns to 1:217/239 of 3kn1A

query
sites
3kn1A

Q

R

I

L

S

T

L

L

L

M

E

E

F

V

L

L

A

G

L

L

E

K

D

D

G

R

G

E

G

G

E

-

F

Y

D

T

N

S

V

F

P

W

E

N

I

D

Y

C

L

I

S

S

C

S

G

G

V

L

A

R

G

G

A

C

T

M

L

L

M

I

D

E

L

L

A

A

L

L

R
|
R

D

G

R
|
R

I

L

D

Q

S

L

D

E

L

A

E

C

G

G

-

M

-

R

-

K

-

S

-

L

-

T

-

R

-

K

V

V

F

I

A

C

V

K

N

S

S

D

A

A

P

P

T

T

G

G

D

D

A

V

I

L

L

L

D

D

R

E

V

A

L

L

A

K

E

H

I

V

V

K

A

E

E

T

P

Q

K

P

R

P

L

E

S

T

P

V

Q

Q

E

N

W

W

I

I

S

E

-

L

-

S

-

G

-

E

-

T

-

W

-

N

-

P

-

L

R

K

L

L

S

H

P

Y

Q

Q

A

L

P

R

A

N

L

V

H

R

K

E

A

R

A

L

L

A

S

K

E

N

L

L

C

V

A

E

R

K

G

G

V

V

L

L

R

T

Q

T

S

E

D

K

H

Q

A

N

F

F

L

L

W

-

V

L

L

F

K

D

E

M

R

T

Y

H

P

P

I

L

V

T

E
x
N

G

N

Q

N

E

I

R

K

-

Q

-
x
R

-

L

-

I

P

K

E

K

A

V

K

Q

R

E

R

A

I

V

L

L

A

D

L

K

L

W

Y

V

N

N

D

D

D

-

L

-

P

P

S

H

P

R

A

M

D

D

-

R

-

L

V

L

A

A

L

L

T

I

A

Y

L

L

A

A

N

H

A

A

C

S

F

D

L

V

F

L

E

E

R

N

I

A

L

F

T

A

P

P

-

L

-

D

K

E

E

Q

L

Y

K

D

R

L

V

A

K

T

P

K

R

R

I

V

E

R

Q

Q

I

L

A

L

R

D

L

L

D

D

binding sulfate ion: R42 (= R58), R44 (= R60), N148 (≠ E145), R154 (vs. gap)

2y5yA Crystal structure of lacy in complex with an affinity inactivator (see paper)
35% identity, 16% coverage: 471:575/640 of query aligns to 24:129/386 of 2y5yA

query
sites
2y5yA

F

F

V

P

Y

F

L

F

S

P

T

I

W

W

L

L

K

H

E

D

Q

I

Q

N

H

H

V

I

A

S

S

K

S

S

-

D

T

T

A

G

L

I

T

I

L

F

N

A

S

A

I

I

A

S

I

L

F

F

A

S

M

L

L

L

I

-

F

F

T

Q

P

P

L

L

A

F

G

G

A

L

L

L

S

S

D

D

R

K

I

L

G

G

R

L

K

R

P

K

V

Y

L

L

I

L

A

W

G

I

A

I

A

T

G

G

M

M

A

L

L

V

F

M

A

F

W

A

P

P

L

F

W

I

I

F

I

I

D

F

T

G

P

P

H

L

L

L

P

Q

A

Y

-

N

I

I

L

L

A

V

G

G

-

S

-

I

L

V

G

G

A

G

F

I

A

Y

L

L

L

G

L

F

S

S

C

F

N

N

G

A

A

G

G
x
C

P

P

A

A

F

-

L

-

V

V

E

E

A

A

F

F

P

I

K

E

H

K

V

V

binding 2-sulfanylethyl beta-D-galactopyranoside: C119 (≠ G563)

Sites not aligning to the query:

binding 2-sulfanylethyl beta-D-galactopyranoside: 17, 148, 252

P36035 Carboxylic acid transporter protein homolog from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
23% identity, 26% coverage: 281:449/640 of query aligns to 186:340/616 of P36035

query
sites
P36035

L

F

A

V

R

R

P

S

L

A

G

G

G

A

L

V

L

I

F

F

G

G

H

L

I

W

G

T

D

D

R

K

L

S

G

S

R

R

R

K

A

W

A

P

V

Y

V

I

S

T

S

C

V

L

V

F

L

L

M

F

I

V

I

I

P

A

T

Q

L

L

F

C

M

T

A

P

L

W

L

C

P

D

T

T

Y

Y

A

E

Q

K

-

F

I

L

G

G

V

V

A

-

P

-

I

-

L

-

V

-

L

-

R

R

L

W

A

I

Q

T

G

G

L

I

A

A

V

M

G

G

E

I

Y

Y

T

G

T

C

S

A

M

S

V

A

L

T

L

A

V

I

E

E

E

D

A

A

Q

P

P

V

S

K

R

A

R

R

G

S

F

F

V

-

G

-

S

-

F

-

A

-

P

-

F

-

G

-

A

-

L

L

G

S

G

G

L

L

L

F

G

S

S

A

A

Y

V

A

G

M

A

G

A

F

L

I

L

F

A

A

I

I

L

I

P

F

E

Y

R

R

D

A

A

F

S

G

T

Y

W

F

-

R

-

D

G

G

W

W

R

K

L

I

A

L

F

F

F

W

S

F

G

S

L

I

L

F

I

L

G

P

L

I

V

L

F

I

L

F

I

W

R

R

R

L

L

W

P

P

P

E

D

T

K

K

K

Y

I

F

L

T

E

K

A

V

E

L

Q

K

A

A

R

R

K

K

S

L

P

I

L

L

G

S

E

D

A

A

F

V

R
x
K

T

A

Q

N

K338 (≠ R447) modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
9 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Q9H4A5 Golgi phosphoprotein 3-like; GPP34-related protein from Homo sapiens (Human) (see paper)
26% identity, 27% coverage: 16:188/640 of query aligns to 45:240/285 of Q9H4A5

query
sites
Q9H4A5

Q

R

I

L

S

T

L

L

L

M

E

E

F

V

L

L

A

G

L

L

E

K

D

D

G

K

G

E

G

G

E

-

F

Y

D

T

N

S

V

F

P

W

E

N

I

D

Y

C

L

I

S

S

C

S

G

G

V

L

A

R

G

G

A

G

T

I

L

L

M

I

D

E

L

L

A

A

L

M

R

R

D

G

R

R

I

I

D

Y

S

-

D

-

L

L

E

E

-

P

-

T

-

M

-

R

-

K

-

R

-

L

-

D

-

R

G

K

V

V

F

L

A

L

V

K

N

S

S

D

A

S

P

P

T

T

G

G

D

D

A

V

I

L

L

L

D

D

R

E

V

T

L

L

A

K

E

H

I

I

V

K

A

A

E

T

P

E

K

P

R

T

L

E

S

T

P

V

Q

Q

E

T

W

W

I

I

S

E

-

L

-

T

-

G

-

E

-

T

-

W

-

N

-

P

-

F

R

K

L

L

S

Q

P

Y

Q

Q

A

L

P

R

A

N

L

V

H

R

K

E

A

R

A

I

L

A

S

K

E

N

L

L

C

V

A

E

R

K

G

G

V

I

L

L

R

T

Q

T

S

E

D

K

H

Q

A

N

F

F

L

L

W

-

V

L

L

F

K

D

E

M

R

T

Y

H

P

P

I

V

V

T

E

N

G

T

Q

T

E

E

R

K

P

Q

E

R

A

L

K

V

R

K

-

L

-

Q

-

D

-

S

I

V

L

L

A

E

L

R

L

W

Y

V

N

N

D

D

D

-

L

-

P

P

S

Q

P

R

A

M

D

D

-

K

-

R

-

T

V

L

A

A

L

L

T

L

A

V

L

L

A

A

N

H

A

S

C

S

F

D

L

V

F

L

E

E

R

N

I

V

L

F

T

S

Sites not aligning to the query:

7:10 RARR→AAAA: Loss of binding to coatomer.

P02920 Lactose permease; Lactose-proton symport from Escherichia coli (strain K12) (see 3 papers)
34% identity, 16% coverage: 471:575/640 of query aligns to 27:132/417 of P02920

query
sites
P02920

F

F

V

P

Y

F

L

F

S

P

T

I

W

W

L

L

K

H

E

D

Q

I

Q

N

H

H

V

I

A

S

S

K

S

S

-

D

T

T

A

G

L

I

T

I

L

F

N

A

S

A

I

I

A

S

I

L

F

F

A

S

M

L

L

L

I

-

F

F

T

Q

P

P

L

L

A

F

G

G

A
x
L

L

L

S

S

D

D

R

K

I

L

G

G

R

L

K

R

P

K

V

Y

L

L

I

L

A

W

G

I

A

I

A

T

G

G

M

M

A

L

L

V

F

M

A

F

W

A

P

P

L

F

W

I

I

F

I

I

D

F

T
x
G

P

P

H

L

L

L

P

Q

-

Y

A

N

I

I

L

L

A

V

G

G

-

S

-

I

L

V

G

G

A

G

F

I

A

Y

L

L

L

G

L

F

S

C

C

F

N

N

G

A

A

G

G
x
A

P

P

A

A

F

-

L

-

V

V

E

E

A

A

F

F

P

I

K

E

H

K

V

V

L65 (≠ A509) mutation to V: No change in transport activity.
G96 (≠ T540) mutation to A: No change in transport activity.
A122 (≠ G563) mutation to S: No change in transport activity.

Sites not aligning to the query:

1 modified: N-formylmethionine; partial
237 mutation D->N,G: Loss of activity.
264 V→A: No change in transport activity.
279 A→S: No change in transport activity.
355 C→Q: No change in transport activity.
358 K→T: Loss of activity.
367 V→A: Increases transport of melibiose and impairs transport of TMG.

A0A0H2VG78 Glucose transporter GlcP; Glucose/H(+) symporter from Staphylococcus epidermidis (strain ATCC 12228 / FDA PCI 1200) (see paper)
24% identity, 46% coverage: 291:583/640 of query aligns to 63:376/446 of A0A0H2VG78

query
sites
A0A0H2VG78

G

G

H

P

I

L

G

A

D

D

R

K

L

L

G

G

R

R

A

R

A

L

V

V

V

M

S

L

S

I

V

A

V

I

L

V

M

F

I

I

P

G

T

A

L

L

F

I

M

L

A

A

L

A

L

S

P

T

T

N

Y

L

A

A

Q

-

I

-

G

-

V

-

A

-

P

-

I

-

L

L

V

I

L

I

L

G

R
|
R

L

L

A

I

Q
x
I

G

G

L

L

A

A

V

V

G

G

E

S

Y

M

T

S

T

T

S

V

M

P

V

V

L

Y

L

L

V

S

E
|
E

E

M

A

A

Q

P

P

T

S

E

R

Y

R

R

G

G

F

S

V

L

G

G

S

S

F

-

A

-

P

-

F

-

G

-

A

-

L

L

G

N

G
x
Q

L

L

L

M

L

I

G

T

S

I

A

G

V

I

G

L

A

A

L

Y

L

L

A

V

I

N

L

Y

P

A

E

F

R

A

D

D

A

I

S

E

T

G

W

W

G

R

W

W

R

M

L

L

A

G

F

L

F

A

S

V

G

V

-

P

-

S

-

V

-

I

L

L

I

V

G

G

L

I

V

Y

V

F

-

M

-

P

-

E

-

S

-

P

-

R

F

W

L

L

I

L

R

E

R

N

R

R

-

N

-

E

-

A

-

R

-

Q

-

V

-

M

-

K

L

I

P

T

P

Y

D

D

K

D

K

S

I

E

L

I

E

D

A

K

E

E

Q

L

A

K

R

E

K

M

S

K

P

E

L

I

G

N

E

A

A

I

F

S

R

E

T

S

Q

T

W

W

R

T

T

V

I

I

-

K

-

S

-

P

-

W

-

L

-

G

-

R

L

I

K

L

V

I

L

V

G

G

-

C

-

I

-

F

-

A

-

I

-

F

-
x
Q

F

F

T

I

L

G

G

I

H
x
N

G

A

V

V

G

I

F

F

Y

Y

L

S

C

S

F

S

V

I

Y

F

L

A

S

K

T

A

W

G

L

L

K

G

E

E

Q

A

H

S

V

I

A

L

S

G

S

S

T

V

A

G

L

I

-

G

T

T

L

I

N

N

S

-

I

-

A

-

I

-

F

-

A

-

M

-

L

V

I

L

F

V

T

T

P

I

L

V

A

A

G

I

A

F

L

V

S

V

D

D

R

K

I

I

G

D

R

R

K

K

P

K

V

L

L

L

I

V

A

G

G

G

A

N

A

I

G

G

M

M

A

I

-

A

-

S

-

L

L

L

F

I

A

M

W

A

P

I

L

L

L

I

W

W

I

T

I

I

D

G

T

I

P

A

H

S

L

S

P

A

A

W

I

I

L

I

A

I

G

V

L

C

G

L

A

S

F

L

A

F

L

I

L

V

L

F

S

F

C

G

N

I

G

S

A

W

G

G

P

P

A

V

F

L

L
x
W

V

V

-

M

-

L

-

P

E

E

A

L

F

F

P

P

K

M

H

R

V

A

R

R

C

G

S

A

G

A

L

T

S

G

I

I

A

S

R102 (= R338) mutation to A: Loss of transport activity.
I105 (≠ Q341) mutation to S: Affects symport activity. May function as an uniporter.
E122 (= E358) mutation to A: Loss of transport activity.
Q137 (≠ G379) mutation to A: Loss of transport activity.
Q250 (vs. gap) mutation to A: Loss of transport activity.
Q251 (vs. gap) mutation to A: Loss of transport activity.
N256 (≠ H463) mutation to A: Loss of transport activity.
W357 (≠ L567) mutation to A: Loss of transport activity.

Sites not aligning to the query:

22 D→N: Affects symport activity. May function as an uniporter.

O15244 Solute carrier family 22 member 2; Organic cation transporter 2; hOCT2 from Homo sapiens (Human) (see 8 papers)
28% identity, 17% coverage: 285:390/640 of query aligns to 162:259/555 of O15244

query
sites
O15244

L

I

G

G

G

S

L
x
M

L

S

F

I

G

G

H
x
Y

I

I

G

A

D

D

R

R

L

F

G

G

R

R

R

K

A

L

A

C

V

L

S

T

V

V

V

L

L

I

M

N

I

A

P

A

T

G

L

V

F

L

M

M

A

A

L

I

L

S

P

P

T

T

Y

Y

A
x
T

Q

-

I

-

G

-

V

-

A

-

P

-

I

-

L

W

L

M

V

L

L

I

L

F

R

R

L

L

A

I

Q

Q

G

G

L

L

A

V

V

S

G

K

G

A

E

G

Y

W

T

L

T

I

S

G

M

Y

V

I

L

L

L

I

V

T

E

E

E

F

A

V

Q

G

P

R

S

R

R

Y

R

R

G

R

F

T

V

V

G

G

S

I

F

F

A
x
Y

P

Q

F

V

G

A

A

Y

L

T

G

V

G

G

L

L

L

V

G

L

S

A

A

G

V

V

G

A

A
x
Y

A

A

L

L

M165 (≠ L288) to I: lower Vmax for MPP(+) transport; no change in transport efficiency (Vmax/Km) and clearance of cyclo(his-pro) and salsolinol; dbSNP:rs8177507
Y169 (≠ H292) mutation to F: No change in TEA uptake.
T201 (≠ A324) to M: in dbSNP:rs145450955
Y241 (≠ A372) mutation to F: Slight decrease in TEA uptake. No change in tyrosine phosphorylation. Strong decrease in TEA uptake; when associated with F-362. Strong decrease in TEA and metformin uptake and YES1-mediated tyrosine phosphorylation; when associated with F-362 and F-377.
Y257 (≠ A388) mutation to F: No change in TEA uptake.

Sites not aligning to the query:

54 P → S: in dbSNP:rs8177504
73 Y→F: No change in TEA uptake.
92 Y→F: No change in TEA uptake.
128 Y→F: No change in TEA uptake.
270 S → A: decreased Ki value for TBA inhibition of MPP(+); no change in transport efficiency (Vmax/Km) and clearance of cyclo(his-pro) and salsolinol; dbSNP:rs316019
279 Y→F: No change in TEA uptake.
280 Y→F: No change in TEA uptake.
284 P→A: Decreased TEA and metformin uptake. Decreased tyrosine phosphorylation.
284:288 Proline-rich sequence
286 S→A: No change in TEA and metformin uptake. No change in tyrosine phosphorylation.
287 P→A: Decreased TEA and metformin uptake. Decreased tyrosine phosphorylation.
362 Y→F: Decreased TEA uptake and YES1-mediated tyrosine phosphorylation. Strong decrease in TEA uptake; when associated with F-241. Strong decrease in TEA uptake; when associated with F-377. Strong decrease in TEA and metformin uptake and YES1-mediated tyrosine phosphorylation; when associated with F-241 and F-377.
377 Y→F: Slight decrease in TEA uptake. No change in tyrosine phosphorylation. Strong decrease in TEA uptake; when associated with F-362. Strong decrease in TEA and metformin uptake and YES1-mediated tyrosine phosphorylation; when associated with F-241 and F-362.
400 R → C: lower Vmax and reduced Ki value for TBA inhibition of MPP(+); lower transport efficiency (Vmax/Km) and clearance of cyclo(his-pro); no change in transport efficiency (Vmax/Km) and clearance of salsolinol; dbSNP:rs8177516
432 K → Q: lower Km value for MPP(+) and reduced Ki value for TBA inhibition of MPP; no change in transport efficiency (Vmax/Km) and clearance of cyclo(his-pro) and salsolinol; dbSNP:rs8177517
458 Y→F: No change in TEA uptake.
544 Y→F: No change in TEA uptake.

Q06385 Vacuolar protein sorting-associated protein 74 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 2 papers)
27% identity, 31% coverage: 6:202/640 of query aligns to 54:287/345 of Q06385

query
sites
Q06385

D

E

R

E

S

S

K

K

S

L

R

R

E

D

T

N

L

I

-

N

-

I

P

P

Q

T

I

L

S

T

L

L

L

M

E

E

F

V

L

L

L

M

A

G

L

L

E

R

D

D

G

R

G

E

G

G

E

-

F

Y

D

L

N

S

V

F

P
x
W

E

N

I

D

Y

S

L

I

S

S

C

Y

G

A

V

L

A
x
R

G

G

A

C

T

I

L

I

M

I

D

E

L

L

A

A

L

L

R

R

D

G

R

K

I

I

-

R

-

I

-

L

-

D

D

D

S

S

-

A

-

R

-

K

-

R

-

F

D

D

L

L

E

S

G

E

-

R

-

L

V

I

F

E

A

V

V

I

N

D

S

S

A

S

P

K

T

T

G

G

D

E

A

V

I

L

L

L

D

D

R

E

V

T

L

L

A

-

E

Q

I

L

V

M

A

K

E

N

P

D

K

E

R

P

L

L

S

S

P

I

Q

S

E

N

W

W

I

I

S

D

-

L

-

S

-

G

-

E

-

T

-

W

-

N

-

L

R

K

L

I

S

N

P

Y

Q

Q

A

L

P
x
K

A

Q

L

V

H
x
R

K

E

A

R

A

L

L

A

S

K

E

G

L

L

C

V

A

D

R

K

G

G

V

V

L

L

R

R

Q

T

S

E

D

M

H

K

A

N

F

F

L

-

W

F

V

L

L

F

K

D

E

M

R

A

R

T

Y

H

P

P

I

I

V

A

E

D

G

A

Q

S

E

C

R

K

P

E

E

A

A

I

K

K

R

R

I

V

L

L

A

S

L

V

L

L

-

V

-

S

-

R

-

N

-

M

-

E

-

L

-

S

Y

Y

N

N

D

E

D

Y

L

F

P

P

S

E

P

T

A

T

D

S

V

F

A

K

L

I

T

I

-

R

A

T

L

L

A

A

N

L

A

I

C

C

-

G

-

S

-

Y

-

G

-

A

F

N

L

V

F

L

E

E

R

N

I

V

L

L

T

T

P

T

K

L

E

E

L

Y

-

E

K

K

R

R

V

D

K

K

-

A

-

I

P

S

R

R

I

A

E

E

Q

E

I

I

W88 (≠ P39) mutation to A: Abolishes phosphoinositide binding and Golgi localization; when associated with A-97.
R97 (≠ A48) mutation to A: Abolishes phosphoinositide binding and Golgi localization; when associated with A-88.
K178 (≠ P109) mutation to A: Abolishes phosphoinositide binding and Golgi localization; when associated with A-181.
R181 (≠ H112) mutation to A: Abolishes phosphoinositide binding and Golgi localization; when associated with A-178.

Sites not aligning to the query:

6:8 RRR→AAA: Loss of coatomer-binding.

4zyrA Crystal structure of e. Coli lactose permease g46w/g262w bound to p- nitrophenyl alpha-d-galactopyranoside (alpha-npg) (see paper)
34% identity, 16% coverage: 471:575/640 of query aligns to 22:127/387 of 4zyrA

query
sites
4zyrA

F

F

V

P

Y

F

L

F

S

P

T

I

W

W

L

L

K

H

E

D

Q

I

Q

N

H

H

V

I

A

S

S

K

S

S

-

D

T

T

A

W

L

I

T

I

L

F

N

A

S

A

I

I

A

S

I

L

F

F

A

S

M

L

L

L

I

-

F

F

T

Q

P

P

L

L

A

F

G

G

A

L

L

L

S

S

D

D

R

K

I

L

G

G

R

L

K

R

P

K

V

Y

L

L

I

L

A

W

G

I

A

I

A

T

G

G

M

M

A

L

L

V

F

M

A

F

W

A

P

P

L

F

W

I

I

F

I

I

D

F

T

G

P

P

H

L

L

L

P

Q

A

Y

-

N

I

I

L

L

A

V

G

G

-

S

-

I

L

V

G

G

A

G

F

I

A

Y

L

L

L

G

L

F

S

C

C

F

N

N

G

A

A

G

G

A

P

P

A

A

F

-

L

-

V

V

E

E

A

A

F

F

P

I

K

E

H

K

V

V

Sites not aligning to the query:

binding 4-nitrophenyl alpha-D-galactopyranoside: 18, 139, 143, 146, 248, 301

6vbgB Lactose permease complex with thiodigalactoside and nanobody 9043 (see paper)
34% identity, 16% coverage: 471:575/640 of query aligns to 27:132/397 of 6vbgB

query
sites
6vbgB

F
|
F

V

P

Y

F

L

F

S

P

T

I

W

W

L

L

K

H

E

D

Q

I

Q

N

H

H

V

I

A

S

S

K

S

S

-

D

T

T

A

W

L

I

T

I

L

F

N

A

S

A

I

I

A

S

I

L

F

F

A

S

M

L

L

L

I

-

F

F

T

Q

P

P

L

L

A

F

G

G

A

L

L

L

S

S

D

D

R

K

I

L

G

G

R

L

K

R

P

K

V

Y

L

L

I

L

A

W

G

I

A

I

A

T

G

G

M

M

A

L

L

V

F

M

A

F

W

A

P

P

L

F

W

I

I

F

I

I

D

F

T

G

P

P

H

L

L

L

P

Q

A

Y

-

N

I

I

L

L

A

V

G

G

-

S

-

I

L

V

G

G

A

G

F

I

A

Y

L

L

L

G

L

F

S

C

C

F

N

N

G

A

A

G

G

A

P

P

A

A

F

-

L

-

V

V

E
|
E

A

A

F

F

P

I

K

E

H

K

V

V

binding 1-thio-beta-D-galactopyranose: F27 (= F471), E126 (= E569)

Sites not aligning to the query:

Query Sequence

>WP_085771389.1 NCBI__GCF_002117405.1:WP_085771389.1
MSLALDRSKSRETLPQISLLEEFLLLALEDGGGEFDNVPEIYLSCGVAGATLMDLALRDR
IDSDLEGVFAVNSAPTGDAILDRVLAEIVAEPKRLSPQEWISRLSPQAPALHKAALSELC
ARGVLRQSDHAFLWVLKERRYPIVEGQERPEAKRRILALLYNDDLPSPADVALTALANAC
FLFERILTPKELKRVKPRIEQIARLDLIGGEILRTAQRVNMETRAAERRTVIAGLAGNVM
EWYDFGIYGFFAAAIGAQFFPSGDPSTSLLASFGVFAAGFLARPLGGLLFGHIGDRLGRR
AAVVSSVVLMIIPTLFMALLPTYAQIGVAAPILLVLLRLAQGLAVGGEYTTSMVLLVEEA
QPSRRGFVGSFAPFGALGGLLLGSAVGAALLAILPERDASTWGWRLAFFSGLLIGLVVFL
IRRRLPPDKKILEAEQARKSPLGEAFRTQWRTILKVLGFTLGHGVGFYLCFVYLSTWLKE
QQHVASSTALTLNSIAIFAMLIFTPLAGALSDRIGRKPVLIAGAAGMALFAWPLLWIIDT
PHLPAILAGLGAFALLLSCNGAGPAFLVEAFPKHVRCSGLSIAYNLAQSIFGGTVPMVAV
FLIKITGNPLAPAFYLAAASALSLAMALLIGRETEAEEAP

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory