SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_085772469.1 NCBI__GCF_002117405.1:WP_085772469.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4yerA Crystal structure of an abc transporter atp-binding protein (tm_1403) from thermotoga maritima msb8 at 2.35 a resolution
35% identity, 85% coverage: 9:224/254 of query aligns to 7:223/285 of 4yerA

query
sites
4yerA

V

V

K

E

S

N

L

L

S

V

H

K

A

K

Y
x
F

G

G

A

D

R
x
F

K

E

A

A

L

V

D

K

D

G

V

V

S

S

L

F

R

S

V

V

E

K

R

K

G

G

A

E

F

I

A

F

V

A

L

F

L

L

G

G

L

P

N
|
N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

F

I

S

H

L

M

A

L

T

T

G

T

L

L

Y

L

G

K

A

P

R

T

A

S

G

G

A

K

V

A

E

W

I

V

M

A

G

G

Y

H

D

D

V

V

A

L

K

K

T

E

P

P

G

R

E

E

A

V

L

R

R

R

R

K

L

I

G

G

V

I

V

V

F

F

Q

Q

A

D

R

Q

T

S

L

L

D

D

L

R

E

E

L

L

S

T

L

A

T

Y

Q

E

N

N

L

M

L

Y

Y

I

H

H

A

G

A

K

L

I

H

Y

G

G

I

Y

G

G

P

G

G

E

E

K

G

L

K

K

R

K

L

R

A

I

G

L

A

E

A

L

L

L

E

E

R

F

A

V

G

E

L

L

S

L

E

E

R

F

A

K

R

D

E

K

K

P

A

V

R

K

D
x
T

L
x
F

S
|
S

G

G

Q

M

M

A

R

R

V

L

E

E

I

I

A

A

R

R

A

S

L

L

L

I

H

H

R

E

P

P

S

E

L

V

L

L

L

F

L

L

D

D

E

E

P

P

T

T

V

I

G

G

L

L

D

D

I

P

K

H

A

T

R

R

A

A

D

H

I

M

L

W

K

E

H

Y

V

I

R

S

G

K

L

M

V

K

A

K

Q

E

E

H

N

N

L

M

A

T

V

I

L

F

W

L

T

T

H

H

L

Y

I

M

D

D

E

E

I

A

A

E

P

Q

-

L

S

A

D

D

Q

R

V

V

I

A

M

I

L

I

H

D

E

H

G

G

R

K

V

I

L

I

T

A

D

L

G

G

S

T

A

P

A

T

E

E

I

L

binding adenosine-5'-diphosphate: F14 (≠ Y16), F17 (≠ R19), N39 (= N41), G40 (= G42), G42 (= G44), K43 (= K45), T44 (≠ S46), T45 (= T47), T135 (≠ D137), F136 (≠ L138), S137 (= S139)

P34358 ABC transporter ced-7; Cell death protein 7 from Caenorhabditis elegans (see 2 papers)
37% identity, 80% coverage: 18:220/254 of query aligns to 559:760/1704 of P34358

query
sites
P34358

A

G

R

E

K

R

A

A

L

V

D

D

D

G

V

L

S

S

L

L

R

R

V

A

E

V

R

R

G

G

A

Q

F

C

A

S

V

I

L

L

G

G

L

H

N

N

G

G

A

A

G

G

K
|
K

S

S

T

T

L

T

F

F

S

S

L

S

A

I

T

A

G

G

L

I

Y

I

G

R

A

P

R

T

A

N

G

G

A

R

V

I

E

T

I

I

M

C

G

G

Y

Y

D

D

V

V

A

G

K

N

T

E

P

P

G

G

E

E

A

T

L

R

R

R

R

H

L

I

G

G

V

M

V

C

F

P

Q

Q

A

Y

R

N

T

P

L

L

D

Y

L

D

E

Q

L

L

S

T

L

V

T

S

Q
x
E

N

H

L

L

L

K

Y

L

H

V

A

Y

A

G

L

L

H

K

G

G

I

A

G

R

P

E

G

K

E

D

G

F

K

K

R

Q

L

D

A

M

G

K

A

R

A

L

L

L

E

S

R

D

A

V

G

K

L

L

S

D

E

F

R

K

A

E

R

N

E

E

K

K

A

A

R

V

D

N

L

L

S

S

G

G

Q

M

M

K

R

R

R

K

V

L

E

C

I

V

A

C

R

M

A

A

L

L

L

I

H

G

R

D

P

S

S

E

L

V

L

L

D

D

E

E

P

P

T

T

V

A

G

G

L

M

D

D

I

P

K

G

A

A

R

R

A

Q

D

D

I

V

L

Q

K

K

H

-

V

-

R

-

G

-

L

L

V

V

A

E

Q

R

E

E

-

K

-

A

N

N

L

R

A

T

V

I

L

L

W

L

T

T

H

H

L

Y

I

M

D

D

E

E

I

A

A

E

P

R

-

L

S

G

D

D

Q

W

V

V

I

F

M

I

L

M

H

S

E

H

G

G

R

K

V

L

L

V

T

A

D

S

G

G

S

T

K586 (= K45) mutation to R: Cell corpses not engulfed.
E639 (≠ Q98) mutation to G: Cell corpses not engulfed.

Sites not aligning to the query:

126 modified: carbohydrate, N-linked (GlcNAc...) asparagine
1012 modified: carbohydrate, N-linked (GlcNAc...) asparagine
1045 modified: carbohydrate, N-linked (GlcNAc...) asparagine
1417 K→R: Some cell corpses not engulfed.

8tzjA Cryo-em structure of vibrio cholerae ftse/ftsx complex (see paper)
36% identity, 76% coverage: 6:198/254 of query aligns to 2:198/220 of 8tzjA

query
sites
8tzjA

A

V

L

I

N

R

V

F

K

Q

S

Q

L

V

S

S

H

K

A

A

Y
|
Y

-

R

G

G

A

G

R
|
R

K

Q

A
|
A

L

L

D

Q

D

K

V

V

S

D

L

F

R

H

V

L

E

R

R

R

G

G

A

E

F

M

A

A

V

F

L

L

L

G

G

G

L

H

N

S

G
|
G

A

A

G
|
G

K
|
K

S
|
S

T

T

L

L

F

L

S

K

L

L

A

I

T

C

G

A

L

I

Y

E

G

R

A

P

R

T

A

D

G

G

A

K

V

I

E

S

I

F

M

N

G

G

Y

H

D

D

V

I

A

T

K

R

T

I

P

P

G

N

E

K

A

D

L

I

-

P

-

F

-

L

-

R

R

R

R

N

L

I

G

G

V

I

V

V

F

F

Q

Q

A

D

R

H

T

R

L

L

D

L

L

M

E

D

L

R

S

S

L

I

T

Y

Q

D

N

N

L

V

L

A

Y

L

H

P

A

M

A

R

L

I

H

E

G

S

I

I

G

S

P

E

G

N

E

E

G

I

K

K

R

R

L

R

A

V

G

S

A

A

L

L

E

D

R

K

A

T

G

G

L

L

S

L

E

D

R

K

A

A

R

R

E

C

K

L

A

P

R

S

D

Q

L

L

S

S

G

G

Q

E

M

Q

R

Q

R

R

V

V

E

G

I

I

A

A

R

R

A

A

L

V

H

N

R

R

P

P

S

T

L

L

L

A

D

D

E

E

P

P

T

T

V

G

G

N

L

L

D

D

I

P

K

E

A

L

R

S

A

S

D

R

I

V

L

L

K

R

H

-

V

-

R

-

G

-

L

L

V

F

A

E

Q

E

E

F

N

N

L

R

A

A

-

G

-

V

-

T

V

I

L

L

W

L

T

A

T

T

H

H

L

D

I

I

binding adenosine-5'-diphosphate: Y12 (= Y16), R16 (= R19), A18 (= A21), G39 (= G42), G41 (= G44), K42 (= K45), S43 (= S46)

P69874 Spermidine/putrescine import ATP-binding protein PotA; EC 7.6.2.11 from Escherichia coli (strain K12) (see 3 papers)
28% identity, 95% coverage: 2:242/254 of query aligns to 13:253/378 of P69874

query
sites
P69874

S

S

E

L

T

S

P

P

A

L

L

V

N

Q

V

L

K

A

S

G

L

I

S

R

H

K

A
x
C

Y
x
F

G

D

A

G

R

K

K

E

A

V

L

I

D

P

D

Q

V

L

S

D

L

L

R

T

V

I

E

N

R

N

G

G

A

E

F
|
F

A

L

V

T

L

L

G

G

L

P

N

S

G

G

A
x
C

G

G

K

K

S

T

T

T

L

V

F
x
L

S

R

L

L

A

I

T

A

G

G

L

L

Y

E

G

T

A

V

R

D

A

S

G

G

A

R

V

I

E

M

I
x
L

M

D

G

N

Y

E

D

D

V

I

A

T

K

H

T

V

P

P

G

A

E

E

A

N

L

-

R

R

R

Y

L

V

G

N

V

T

V

V

F

F

Q

Q

A

S

R

Y

T

A

L

L

D

F

L

P

E

H

L

M

S

T

L

V

T

F

Q

E

N

N

L

V

L

A

Y

F

H

G

A

L

A

R

L

M

H

Q

G

K

I

T

G

P

P

A

G

A

E

E

G

I

K

T

R

P

L

R

A

V

G

M

A

E

A

A

L

L

E

R

R

M

A
x
V

G

Q

L

L

S

E

E

T

R

F

A

A

R

Q

E

R

K

K

A

P

R

H

D

Q

L

L

S

S

G

G

Q

Q

M

Q

R

Q

R

R

V

V

E

A

I

I

A

A

R

R

A

A

L

V

H

N

R

K

P

P

S

R

L

L

D
|
D

E

E

P

S

T

L

V

S

G

A

L

L

D

D

I

Y

K

K

A

L

R

R

A

K

D

Q

I

M

L

Q

K

N

H

E

V

L

R

K

G

A

L

L

V

Q

A

R

Q

K

E

L

N

G

L

I

A

T

V

F

L

V

W

F

T

V

T

T

H

H

L

D

I

Q

D

E

E

E

-

A

I

L

A

T

P

M

S

S

D

D

Q

R

V

I

I

V

M

V

L

M

H

R

E

D

G

G

R

R

V

I

L

E

T

Q

D

D

G

G

S

T

A

P

A

R

E

E

I

I

V

Y

A

E

Q

P

E

K

T

N

D

L

S

F

I

V

G

A

G

G

A

F

F

I

E

G

K

E

L

I

T

N

M

M

C26 (≠ A15) mutation to A: Lower ATPase activity and transport efficiency.
F27 (≠ Y16) mutation to L: Lower ATPase activity and transport efficiency.
F45 (= F34) mutation to L: Lower ATPase activity and transport efficiency.
C54 (≠ A43) mutation to T: Loss of ATPase activity and transport.
L60 (≠ F49) mutation to F: Lower ATPase activity and transport efficiency.
L76 (≠ I65) mutation to P: Lower ATPase activity and transport efficiency.
V135 (≠ A125) mutation to M: Loss of ATPase activity and transport.
D172 (= D162) mutation to N: Loss of ATPase activity and transport.

Sites not aligning to the query:

276 C→A: Lower ATPase activity and transport efficiency.
297 mutation E->K,D: Lower ATPase activity and transport efficiency.; E→Q: Loss of ATPase activity and transport.

8y5iA Cryo-em structure of e.Coli spermidine transporter potd-potabc in translocation intermidiate state (see paper)
27% identity, 94% coverage: 5:242/254 of query aligns to 1:238/358 of 8y5iA

query
sites
8y5iA

P

P

A

L

L

V

N

Q

V

L

K

A

S

G

L

I

S

R

H

K

A

C

Y
x
F

G

D

A

G

R
x
K

K

E

A

V

L

I

D

P

D

Q

V

L

S

D

L

L

R

T

V

I

E

N

R

N

G

G

A

E

F

F

A

L

V

T

L

L

G

G

L

P

N

S

G
|
G

A

C

G

G

K
|
K

S
x
T

T
|
T

L

V

F

L

S

R

L

L

A

I

T

A

G

G

L

L

Y

E

G

T

A

V

R

D

A

S

G

G

A

R

V

I

E

M

I

L

M

D

G

N

Y

E

D

D

V

I

A

T

K

H

T

V

P

P

G

A

E

E

A

N

L

-

R

R

R

Y

L

V

G

N

V

T

V

V

F

F

Q

Q

A

S

R

Y

T

A

L

L

D

F

L

P

E

H

L

M

S

T

L

V

T

F

Q

E

N

N

L

V

L

A

Y

F

H

G

A

L

A

R

L

M

H

Q

G

K

I

T

G

P

P

A

G

A

E

E

G

I

K

T

R

P

L

R

A

V

G

M

A

E

A

A

L

L

E

R

R

M

A

V

G

Q

L

L

S

E

E

T

R

F

A

A

R

Q

E
x
R

K

K

A

P

R

H

D
x
Q

L

L

S
|
S

G

G

Q

Q

M

Q

R

Q

R

R

V

V

E

A

I

I

A

A

R

R

A

A

L

V

H

N

R

K

P

P

S

R

L

L

D

D

E

Q

P

S

T

L

V

S

G

A

L

L

D

D

I

Y

K

K

A

L

R

R

A

K

D

Q

I

M

L

Q

K

N

H

E

V

L

R

K

G

A

L

L

V

Q

A

R

Q

K

E

L

N

G

L

I

A

T

V

F

L

V

W

F

T

V

T

T

H

H

L

D

I

Q

D

E

E

E

-

A

I

L

A

T

P

M

S

S

D

D

Q

R

V

I

I

V

M

V

L

M

H

R

E

D

G

G

R

R

V

I

L

E

T

Q

D

D

G

G

S

T

A

P

A

R

E

E

I

I

V

Y

A

E

Q

P

E

K

T

N

D

L

S

F

I

V

G

A

G

G

A

F

F

I

E

G

K

E

L

I

T

N

M

M

binding adenosine-5'-triphosphate: F12 (≠ Y16), K15 (≠ R19), G38 (= G42), K41 (= K45), T42 (≠ S46), T43 (= T47), R128 (≠ E133), Q132 (≠ D137), S134 (= S139)

8bmpA Cryo-em structure of the folate-specific ecf transporter complex in msp2n2 lipid nanodiscs bound to atp and adp (see paper)
32% identity, 87% coverage: 8:229/254 of query aligns to 4:232/278 of 8bmpA

query
sites
8bmpA

N

N

V

I

K

I

S

S

L

F

S

D

H

H

A

V

Y

T

-
x
F

-

D

G

S

A

P

R

R

K

P

A

A

L

L

D

S

D

D

V

L

S

S

L

F

R

A

V

I

E

E

R

R

G

G

A

S

F

W

A

T

V

A

L

L

L

I

G

G

L

H

N
|
N

G

G

A

S

G
|
G

K
|
K

S
|
S

T
|
T

L

V

F

S

S

K

L

L

A

I

T

N

G

G

L

L

Y

L

G

A

A

P

R

D

-

D

-

L

-

D

A

K

G

S

A

S

V

I

E

T

I

V

M

D

G

G

Y

V

D

K

V

L

-

G

A

A

K

D

T

T

P

V

G

W

E

E

A

V

L

R

R

E

R

K

L

V

G

G

V

I

V

V

F

F

Q

Q

A

-

R

-

T

N

L

P

D

D

L

N

E

Q

L

F

-

V

-

G

-

A

S

T

L

V

T

S

Q

D

N

D

L

V

L

A

Y

F

H

G

A

L

A

E

L

N

H

R

G

A

I

V

G

P

P

R

G

P

E

E

G

M

K

L

R

K

L

I

A

V

G

A

A

Q

A

A

L

V

E

A

R

D

A

V

G

G

L

M

S

A

E

D

R

Y

A

A

R

D

E

S

K

E

A

P

R

S

D

N

L

L

S

S

G

G

Q

Q

M

K

R

Q

R

R

V

V

E

A

I

I

A

A

R

G

A

I

L

L

L

A

H

V

R

K

P

P

S

Q

L

V

L

I

L

L

D

D

E

E

P

S

T

T

V

S

G

M

L

L

D

D

I

P

K

E

A

G

R

K

A

E

D

Q

I

I

L

L

K

D

H

L

V

V

R

R

G

K

L

I

V

K

A

E

Q

D

E

N

N

N

L

L

A

T

V

V

L

I

W

S

T

I

T

T

H

H

L

D

I

L

D

E

E

E

I

A

A

A

P

G

S

A

D

D

Q

Q

V

V

I

L

M

V

L

L

H

D

E

D

G

G

R

Q

V

L

L

L

T

D

D

Q

G

G

S

K

A

P

A

E

E

E

I

I

V

F

A

P

A

K

Q

V

E

E

binding adenosine-5'-triphosphate: F13 (vs. gap), N39 (= N41), G42 (= G44), K43 (= K45), S44 (= S46), T45 (= T47)

5d3mA Folate ecf transporter: amppnp bound state (see paper)
31% identity, 86% coverage: 11:229/254 of query aligns to 12:235/280 of 5d3mA

query
sites
5d3mA

S

T

L
x
F

S

T

H

Y

A

P

Y

D

G

S

A

P

R

R

K

P

A

A

L

L

D

S

D

D

V

L

S

S

L

F

R

A

V

I

E

E

R

R

G

G

A

S

F

W

A

T

V

A

L

L

L

I

G

G

L

H

N
|
N

G
|
G

A

S

G

G

K
|
K

S
|
S

T

T

L

V

F

S

S

K

L

L

A

I

T

N

G

G

L

L

Y

L

G

A

A

P

R

D

-

D

-

L

-

D

A

K

G

S

A

S

V

I

E

T

I

V

M

D

G

G

Y

V

D

K

V

L

-

G

A

A

K

D

T

T

P

V

G

W

E

E

A

V

L

R

R

E

R

K

L

V

G

G

V

I

V

V

F

F

Q

Q

A

-

R

-

T

N

L

P

D

D

L

N

E

Q

L

F

-

V

-

G

-

A

S

T

L

V

T

S

Q

D

N

D

L

V

L

A

Y

F

H

G

A

L

A

E

L

N

H

R

G

A

I

V

G

P

P

R

G

P

E

E

G

M

K

L

R

K

L

I

A

V

G

A

A

Q

A

A

L

V

E

A

R

D

A

V

G

G

L

M

S

A

E

D

R

Y

A

A

R

D

E

S

K

E

A

P

R

S

D

N

L

L

S

S

G

G

Q

Q

M

K

R

Q

R

R

V

V

E

A

I

I

A

A

R

G

A

I

L

L

L

A

H

V

R

K

P

P

S

Q

L

V

L

I

L

L

D

D

E

E

P

S

T

T

V

S

G

M

L

L

D

D

I

P

K

E

A

G

R

K

A

E

D

Q

I

I

L

L

K

D

H

L

V

V

R

R

G

K

L

I

V

K

A

E

Q

D

E

N

N

N

L

L

A

T

V

V

L

I

W

S

T

I

T

T

H

H

L

D

I

L

D

E

E

E

I

A

A

A

P

G

S

A

D

D

Q

Q

V

V

I

L

M

V

L

L

H

D

E

D

G

G

R

Q

V

L

L

L

T

D

D

Q

G

G

S

K

A

P

A

E

E

E

I

I

V

F

A

P

A

K

Q

V

E

E

binding phosphoaminophosphonic acid-adenylate ester: F13 (≠ L12), N42 (= N41), G43 (= G42), K46 (= K45), S47 (= S46)

8bmsA Cryo-em structure of the mutant solitary ecf module 2eq in msp2n2 lipid nanodiscs in the atpase closed and atp-bound conformation (see paper)
31% identity, 87% coverage: 8:229/254 of query aligns to 3:232/278 of 8bmsA

query
sites
8bmsA

N

N

V

I

K

I

S

S

L

F

S

D

H

H

A

V

Y

T

-
x
F

-

T

-

D

G

S

A

P

R

R

K

P

A

A

L

L

D

S

D

D

V

L

S

S

L

F

R

A

V

I

E

E

R

R

G

G

A

S

F

W

A

T

V

A

L

L

L

I

G

G

L

H

N
|
N

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

V

F

S

S

K

L

L

A

I

T

N

G

G

L

L

Y

L

G

A

A

P

R

D

-

D

-

L

-

D

A

K

G

S

A

S

V

I

E

T

I

V

M

D

G

G

Y

V

D

K

V

L

-

G

A

A

K

D

T

T

P

V

G

W

E

E

A

V

L

R

R

E

R

K

L

V

G

G

V

I

V

V

F

F

Q
|
Q

A

-

R

-

T

N

L

P

D

D

L

N

E

Q

L

F

-

V

-

G

-

A

S

T

L

V

T

S

Q

D

N

D

L

V

L

A

Y

F

H

G

A

L

A

E

L

N

H

R

G

A

I

V

G

P

P

R

G

P

E

E

G

M

K

L

R

K

L

I

A

V

G

A

A

Q

A

A

L

V

E

A

R

D

A

V

G

G

L

M

S

A

E

D

R
x
Y

A

A

R

D

E

S

K

E

A

P

R

S

D
x
N

L

L

S
|
S

G
|
G

Q
|
Q

M

K

R

Q

R

R

V

V

E

A

I

I

A

A

R

G

A

I

L

L

L

A

H

V

R

K

P

P

S

Q

L

V

L

I

L

L

D

D

E
x
Q

P

S

T

T

V

S

G

M

L

L

D

D

I

P

K

E

A

G

R

K

A

E

D

Q

I

I

L

L

K

D

H

L

V

V

R

R

G

K

L

I

V

K

A

E

Q

D

E

N

N

N

L

L

A

T

V

V

L

I

W

S

T

I

T

T

H
|
H

L

D

I

L

D

E

E

E

I

A

A

A

P

G

S

A

D

D

Q

Q

V

V

I

L

M

V

L

L

H

D

E

D

G

G

R

Q

V

L

L

L

T

D

D

Q

G

G

S

K

A

P

A

E

E

E

I

I

V

F

A

P

A

K

Q

V

E

E

binding adenosine-5'-triphosphate: F12 (vs. gap), N39 (= N41), G40 (= G42), S41 (≠ A43), G42 (= G44), K43 (= K45), S44 (= S46), T45 (= T47), Q88 (= Q86), Y133 (≠ R130), N140 (≠ D137), S142 (= S139), G143 (= G140), G144 (= G141), Q145 (= Q142), Q166 (≠ E163), H199 (= H196)
binding magnesium ion: S44 (= S46), Q88 (= Q86)

8f5bA Human abca4 structure in complex with amp-pnp (see paper)
30% identity, 85% coverage: 5:220/254 of query aligns to 719:935/1924 of 8f5bA

query
sites
8f5bA

P

P

A

G

L

V

N

C

V

V

K

K

S

N

L

L

S

V

H

K

A

I

Y

F

G

E

-

P

-

C

A

G

R

R

K

P

A

A

L

V

D

D

D

R

V

L

S

N

L

I

R

T

V

F

E

Y

R

E

G

N

A

Q

F

I

A

T

V

A

L

F

L

L

G

G

L

H

N
|
N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

F

L

S

S

L

I

A

L

T

T

G

G

L

L

Y

L

G

P

A

P

R

T

A

S

G

G

A

T

V

V

E

L

I

V

M

G

G

G

Y

R

D

D

V

I

A

E

K

T

T

S

P

L

G

D

E

A

A

V

L

R

R

Q

R

S

L

L

G

G

V

M

V

C

F

P

Q
|
Q

A

H

R

N

T

I

L

L

D

F

L

H

E

H

L

L

S

T

L

V

T

A

Q

E

N

H

L

M

L

L

Y

F

H

Y

A

A

A

Q

L

L

H

K

G

G

I

K

G

S

P

Q

G

E

E

E

G

A

K

Q

R

L

L

E

A

M

G

E

A

A

A

M

L

L

E

E

R

D

A

T

G

G

L

L

S

H

E

H

R

K

A

R

R

N

E

E

K

E

A

A

R

Q

D
|
D

L

L

S

S

G

G

Q

M

M

Q

R

R

R

K

V

L

E

S

I

V

A

A

R

I

A

A

L

F

L

V

H

G

R

D

P

A

S

K

L

V

L

I

L

L

D

D

E

E

P

P

T

T

V

S

G

G

L

V

D

D

I

P

K

Y

A

S

R

R

A

R

D

S

I

I

L

W

K

D

H

L

V

L

R

-

G

-

L

L

V

K

A

Y

Q

R

E

S

N

G

L

R

A

T

V

I

L

I

W

M

T

S

T

T

H

H

L

H

I

M

D

D

E

E

I

A

-

D

A

L

P

L

S

G

D

D

Q

R

V

I

I

A

M

I

L

I

H

A

E

Q

G

G

R

R

V

L

L

Y

T

C

D

S

G

G

S

T

binding phosphoaminophosphonic acid-adenylate ester: N757 (= N41), G758 (= G42), G760 (= G44), K761 (= K45), T762 (≠ S46), T763 (= T47), Q802 (= Q86), D853 (= D137)
binding magnesium ion: T762 (≠ S46), Q802 (= Q86)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 1652, 1653, 1654, 1655, 1656, 1657, 1697, 1748, 1750
binding magnesium ion: 1657, 1697

Q5M244 Energy-coupling factor transporter ATP-binding protein EcfA2; ECF transporter A component EcfA2; EC 3.6.3.- from Streptococcus thermophilus (strain ATCC BAA-250 / LMG 18311) (see paper)
29% identity, 86% coverage: 7:224/254 of query aligns to 3:232/280 of Q5M244

query
sites
Q5M244

L

I

N

S

V

L

K

E

S

N

L

V

S

S

H

Y

A

T

Y

Y

G

Q

A

S

-

G

-

T

-

P

-

F

-

E

R

R

K

R

A

A

L

L

D

F

D

D

V

M

S

T

L

V

R

T

V

I

E

K

R

D

G

G

A

S

F

Y

A

T

V

A

L

F

L

I

G

G

L

H

N

T

G

G

A

S

G

G

K

K

S

S

T

T

L

I

F

M

S

Q

L

L

A

L

T

N

G

G

L

L

Y

Y

G

L

A

P

R

T

A

S

G

G

A

Q

V

V

E

K

-

V

-

D

-

T

-

I

I

I

M

N

G

S

Y

Q

D

S

V

K

A

N

K

K

T

E

P

I

G

K

E

P

A

I

L

R

R

K

L

V

G

G

V

L

V

V

F

F

Q

Q

A

F

R

P

T

E

L

S

D
x
Q

L

L

E

F

L

A

-

E

S

T

L

V

T

L

Q

E

N

D

L

I

L

A

Y

F

H

G

A

P

A

Q

L

N

H

F

G

G

I

V

G

S

P

K

G

E

E

E

G

A

K

E

R

Q

L

R

A

A

G

L

A

E

A

S

L

L

E

R

R

L

A

V

G

G

L

L

S

S

E

D

R

E

A

L

R

R

E

D

K

Q

-

N

A

P

R

F

D

D

L

L

S

S

G

G

Q

Q

M

M

R

R

V

V

E

A

I

I

A

A

R

G

A

I

L

L

L

A

H

M

R

Q

P

P

S

D

L

I

L

L

L

V

L

L

D

D

E
|
E

P

P

T

T

V

A

G

G

L

L

D

D

I

P

K

Q

A

G

R

R

A

K

D

E

I

L

L

M

K

S

H

L

V

F

R

K

G

Q

L

L

V

-

A

H

Q

L

E

S

N

G

L

I

A

T

V

I

L

V

W

L

T

V

T

T

H

H

L

L

I

M

D

D

E

D

I

V

A

A

P

D

-

Y

S

A

D

T

Q

A

V

V

I

N

M

V

L

M

H

E

E

K

G

G

R

R

V

L

L

V

T

L

D

S

G

G

S

T

A

P

A

K

E

D

I

V

Q97 (≠ D91) mutation to A: No effect on ATPase, 10-fold decrease in riboflavin uptake; when associated with A-90 in EcfA1.
E171 (= E163) mutation to Q: 10-fold decrease in ATPase and riboflavin uptake; when associated with Q-163 in EcfA1.

5lilA Structure of aggregatibacter actinomycetemcomitans macb bound to atpys (p21) (see paper)
28% identity, 89% coverage: 7:233/254 of query aligns to 4:238/615 of 5lilA

query
sites
5lilA

L

I

N

E

V

I

K

K

S

Q

L

L

S

N

H

R

A

Y

Y
x
F

G

G

A

E

R

G

K

E

-

N

-

R

-

V

-

H

A
x
V

L

L

D

K

D

D

V

I

S

S

L

L

R

S

V

I

E

E

R

R

G

G

A

D

F

F

A

V

V

A

L

I

L

M

G

G

L

Q

N
x
S

G
|
G

A

S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

F

M

S

N

L

I

A

I

T

G

G

C

L

L

Y

D

G

T

A

A

R

T

A

G

G

G

A

S

V

S

E

K

I

I

M

D

G

G

Y

K

D

E

V

T

A

I

K

E

T

L

P

T

G

N

E

D

A

Q

L

L

-

S

-

D

-

L

-

R

-

S

R

Q

R

K

L

F

G

G

V

F

V

I

F

F

Q
|
Q

A

R

R

Y

T

N

L

L

D

L

L

S

E

S

L

L

S

T

L

A

T

A

Q

E

N

N

L

V

L

A

Y

L

H

P

A

A

A

I

L

Y

H

A

G

G

I

M

G

P

P

Q

G

S

E

Q

G

R

K

L

R

E

L

R

A

A

G

K

A

Q

A

L

L

L

E

E

R

K

A

L

G

G

L

L

S

G

E

D

R
x
K

A

W

R

Q

E

N

K

K

A

P

R

N

D
x
Q

L

L

S
|
S

G

G

G
|
G

Q
|
Q

M

Q

R

Q

R

R

V

V

E

S

I

I

A

A

R

R

A

A

L

L

L

M

H

N

R

G

P

G

S

E

L

I

L

L

L

A

D

D

E

Q

P

P

T

T

V

G

G

A

L

L

D

D

I

S

K

H

A

S

R

G

A

E

D

N

I

V

L

M

K

E

H

I

V

L

R

R

G

Q

L

L

V

-

A

H

Q

E

E

E

N

G

L

H

A

T

V

I

L

I

W

M

T

V

T

T

H

H

L

D

I

K

D

H

E

I

I

A

A

A

P

S

S

A

D

N

Q

R

V

I

I

I

M

E

L

I

H

K

E

D

G

G

R

E

V

I

L

I

T

S

D

D

G

T

S

Q

A

K

A

R

E

Q

I

V

V

K

A

S

A

A

Q

V

E

K

T

N

D

P

S

S

I

V

binding adenosine-5'-triphosphate: F13 (≠ Y16), V22 (≠ A21), S42 (≠ N41), G43 (= G42), G45 (= G44), K46 (= K45), S47 (= S46), T48 (= T47), Q92 (= Q86), K136 (≠ R130), Q143 (≠ D137), S145 (= S139), G147 (= G141), Q148 (= Q142)
binding magnesium ion: S47 (= S46), Q92 (= Q86)

5lj7A Structure of aggregatibacter actinomycetemcomitans macb bound to atp (p21) (see paper)
28% identity, 89% coverage: 7:233/254 of query aligns to 4:238/592 of 5lj7A

query
sites
5lj7A

L

I

N

E

V

I

K

K

S

Q

L

L

S

N

H

R

A

Y

Y

F

G

G

A

E

R

G

K

E

-

N

-

R

-

V

-

H

A
x
V

L

L

D

K

D

D

V

I

S

S

L

L

R

S

V

I

E

E

R

R

G

G

A

D

F

F

A

V

V

A

L

I

L

M

G

G

L

Q

N
x
S

G
|
G

A

S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

F

M

S

N

L

I

A

I

T

G

G

C

L

L

Y

D

G

T

A

A

R

T

A

G

G

G

A

S

V

S

E

K

I

I

M

D

G

G

Y

K

D

E

V

T

A

I

K

E

T

L

P

T

G

N

E

D

A

Q

L

L

-

S

-

D

-

L

-

R

-

S

R

Q

R

K

L

F

G

G

V

F

V

I

F

F

Q
|
Q

A

R

R

Y

T

N

L

L

D

L

L

S

E

S

L

L

S

T

L

A

T

A

Q

E

N

N

L

V

L

A

Y

L

H

P

A

A

A

I

L

Y

H

A

G

G

I

M

G

P

P

Q

G

S

E

Q

G

R

K

L

R

E

L

R

A

A

G

K

A

Q

A

L

L

L

E

E

R

K

A

L

G

G

L

L

S

G

E

D

R
x
K

A

W

R

Q

E

N

K

K

A

P

R

N

D
x
Q

L

L

S
|
S

G

G

G
|
G

Q
|
Q

M

Q

R

Q

R

R

V

V

E

S

I

I

A

A

R

R

A

A

L

L

L

M

H

N

R

G

P

G

S

E

L

I

L

L

L

A

D

D

E

Q

P

P

T

T

V

G

G

A

L

L

D

D

I

S

K

H

A

S

R

G

A

E

D

N

I

V

L

M

K

E

H

I

V

L

R

R

G

Q

L

L

V

-

A

H

Q

E

E

E

N

G

L

H

A

T

V

I

L

I

W

M

T

V

T

T

H

H

L

D

I

K

D

H

E

I

I

A

A

A

P

S

S

A

D

N

Q

R

V

I

I

I

M

E

L

I

H

K

E

D

G

G

R

E

V

I

L

I

T

S

D

D

G

T

S

Q

A

K

A

R

E

Q

I

V

V

K

A

S

A

A

Q

V

E

K

T

N

D

P

S

S

I

V

binding adenosine-5'-triphosphate: V22 (≠ A21), S42 (≠ N41), G43 (= G42), G45 (= G44), K46 (= K45), S47 (= S46), T48 (= T47), Q92 (= Q86), K136 (≠ R130), Q143 (≠ D137), S145 (= S139), G147 (= G141), Q148 (= Q142)
binding magnesium ion: S47 (= S46), Q92 (= Q86)

8w6iD Cryo-em structure of escherichia coli str k12 ftsex complex with atp- gamma-s in peptidisc
33% identity, 77% coverage: 7:202/254 of query aligns to 2:201/219 of 8w6iD

query
sites
8w6iD

L

I

N

R

V

F

K

E

S

H

L

V

S

S

H

K

A

A

Y
|
Y

-

L

G

G

A

G

R

R

K

Q

A

A

L

L

D

Q

D

G

V

V

S

T

L

F

R

H

V

M

E

Q

R

P

G

G

A

E

F

M

A

A

V

F

L

L

L

T

G

G

L

H

N
x
S

G
|
G

A
|
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

F

L

S

K

L

L

A

I

T

C

G

G

L

I

Y

E

G

R

A

P

R

S

A

A

G

G

A

K

V

I

E

W

I

F

M

S

G

G

Y

H

D

D

V

I

A

T

K

R

T

L

P

K

G

N

E

R

A

E

L

V

-

P

-

F

-

L

-

R

R

R

R

Q

L

I

G

G

V

M

V

I

F

F

Q
|
Q

A

D

R

H

T

H

L

L

D

L

L

M

E

D

L

R

S

T

L

V

T

Y

Q

D

N

N

L

V

L

A

Y

I

H

P

A

L

A

I

L

I

H

A

G

G

I

A

G

S

P

G

G

D

E

D

G

I

K

R

R

R

L

R

A

V

G

S

A

A

L

L

E

D

R

K

A

V

G

G

L

L

S

L

E

D

R
x
K

A

A

R

K

E

N

K

F

A

P

R

I

D
x
Q

L

L

S
|
S

G
|
G

Q
x
E

M

Q

R

Q

R

R

V

V

E

G

I

I

A

A

R

R

A

A

L

V

H

N

R

K

P

P

S

A

L

V

L

L

L

A

D

D

E

E

P

P

T

T

V

G

G

N

L

L

D

D

I

D

K

A

A

L

R

S

A

E

D

G

I

I

L

L

K

R

H

-

V

-

R

-

G

-

L

L

V

F

A

E

Q

E

E

F

N

N

-

R

-

V

-

G

L

V

A

T

V

V

L

L

W

M

T

A

T

T

H
|
H

L

D

I

I

D

N

E

L

I

I

A

S

binding phosphothiophosphoric acid-adenylate ester: Y11 (= Y16), S37 (≠ N41), G38 (= G42), A39 (= A43), G40 (= G44), K41 (= K45), S42 (= S46), T43 (= T47), Q86 (= Q86), K130 (≠ R130), Q137 (≠ D137), S139 (= S139), G140 (= G140), G141 (= G141), E142 (≠ Q142), H195 (= H196)

P0A9R7 Cell division ATP-binding protein FtsE from Escherichia coli (strain K12) (see paper)
33% identity, 77% coverage: 7:202/254 of query aligns to 2:201/222 of P0A9R7

query
sites
P0A9R7

L

I

N

R

V

F

K

E

S

H

L

V

S

S

H

K

A

A

Y

Y

-

L

G

G

A

G

R

R

K

Q

A

A

L

L

D

Q

D

G

V

V

S

T

L

F

R

H

V

M

E

Q

R

P

G

G

A

E

F

M

A

A

V

F

L

L

L

T

G

G

L

H

N

S

G

G

A

A

G

G

K
|
K

S

S

T

T

L

L

F

L

S

K

L

L

A

I

T
x
C

G

G

L

I

Y

E

G

R

A

P

R

S

A

A

G

G

A

K

V

I

E

W

I

F

M

S

G

G

Y

H

D

D

V

I

A

T

K

R

T

L

P

K

G

N

E

R

A

E

L

V

-

P

-

F

-

L

-

R

R

R

R

Q

L

I

G

G

V

M

V

I

F

F

Q

Q

A

D

R

H

T

H

L

L

D

L

L

M

E

D

L

R

S

T

L

V

T

Y

Q

D

N

N

L

V

L

A

Y

I

H

P

A

L

A

I

L

I

H

A

G

G

I

A

G

S

P

G

G

D

E

D

G

I

K

R

R

R

L

R

A

V

G

S

A

A

L

L

E

D

R

K

A

V

G

G

L

L

S

L

E

D

R

K

A

A

R

K

E

N

K

F

A

P

R

I

D

Q

L

L

S

S

G

G

Q

E

M

Q

R

Q

R

R

V

V

E

G

I

I

A

A

R

R

A

A

L

V

H

N

R

K

P

P

S

A

L

V

L

L

L

A

D

D

E

E

P

P

T

T

V

G

G

N

L

L

D

D

I

D

K

A

A

L

R

S

A

E

D

G

I

I

L

L

K

R

H

-

V

-

R

-

G

-

L

L

V

F

A

E

Q

E

E

F

N

N

-

R

-

V

-

G

L

V

A

T

V

V

L

L

W

M

T

A

T

T

H

H

L

D

I

I

D

N

E

L

I

I

A

S

K41 (= K45) mutation to R: Does not bind ATP.
C49 (≠ T53) mutation to A: Prevents dimer formation. Does not alter ATP-binding.

5x40A Structure of a cbio dimer bound with amppcp (see paper)
34% identity, 89% coverage: 4:230/254 of query aligns to 2:233/280 of 5x40A

query
sites
5x40A

T

T

P

P

A

I

L

L

N

A

V

A

K

E

S

A

L

L

S

T

H

Y

A

A

Y
x
F

-

P

G

G

A

G

R
x
V

K

K

A
|
A

L

L

D

D

V

L

S

S

L

L

R

A

V

V

E

P

R

K

G

G

A

E

F

S

A

L

V

A

L

I

L

L

G

G

L

P

N
|
N

G
|
G

A

A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

F

L

S

L

L

H

A

L

T

N

G

G

L

T

Y

L

G

R

A

P

R

Q

A

S

G

G

A

R

V

V

E

L

I

L

M

G

G

G

Y

T

D

A

V

T

A

G

K

H

T

S

P

R

G

K

E

D

A

L

L

T

-

G

-

W

-

R

R

R

L

V

G

G

V

L

V

V

F

L

Q
|
Q

-

D

A

A

R

D

T

D

L

Q

D

L

L

F

E

A

L

T

S

T

L

V

T

F

Q

E

N

D

L

V

L

S

Y

F

H

G

A

P

A

L

L

N

H

L

G

G

I

L

G

S

P

E

G

A

E

E

G

A

K

R

R

A

L

R

A

V

G

E

A

E

A

A

L

L

E

A

R

A

A

L

G

S

L

I

S

S

E

D

R

L

A

R

R

D

E

R

K

P

A

T

R
x
H

D
x
M

L
|
L

S
|
S

G

G

G
|
G

Q
|
Q

M

K

R

R

V

V

E

A

I

I

A

A

R

G

A

A

L

V

L

A

H

M

R

R

P

P

S

E

L

V

L

L

D

D

E
x
Q

P

P

T

T

V

A

G

G

L

L

D

D

I

L

K

A

A

G

R

T

A

E

D

Q

I

L

L

L

K

T

H

L

V

L

R

R

G

G

L

L

V

R

A

A

Q

A

E

-

N

G

L

M

A

T

V

L

L

V

W

F

T

S

T

T

H
|
H

L

D

I

V

D

E

E

L

I

A

A

A

P

A

-

L

S

A

D

D

Q

R

V

V

I

A

M

L

L

F

H

R

E

T

G

G

R

R

V

V

L

L

T

A

D

E

G

G

S

A

A

A

A

E

E

A

I

V

V

L

A

S

A

D

Q

R

E

A

T

T

binding phosphomethylphosphonic acid adenylate ester: F14 (≠ Y16), V18 (≠ R19), A20 (= A21), N40 (= N41), G41 (= G42), G43 (= G44), K44 (= K45), S45 (= S46), T46 (= T47), Q88 (= Q86), H139 (≠ R136), M140 (≠ D137), L141 (= L138), S142 (= S139), G144 (= G141), Q145 (= Q142), Q166 (≠ E163), H198 (= H196)
binding magnesium ion: S45 (= S46), Q88 (= Q86)

Q99758 Phospholipid-transporting ATPase ABCA3; ABC-C transporter; ATP-binding cassette sub-family A member 3; ATP-binding cassette transporter 3; ATP-binding cassette 3; Xenobiotic-transporting ATPase ABCA3; EC 7.6.2.1; EC 7.6.2.2 from Homo sapiens (Human) (see 15 papers)
30% identity, 85% coverage: 7:221/254 of query aligns to 530:747/1704 of Q99758

query
sites
Q99758

L

I

N

K

V

I

K

K

S

H

L

L

S

S

H

K

A

V

Y

F

G

R

A

V

-

G

-

N

-

K

-

D

R

R

K

A

A

A

L

V

D

R

D

D

V

L

S

N

L

L

R

N

V

L

E

Y

R

E

G

G

A

Q

F

I

A

T

V

V

L

L

G

G

L

H

N
|
N

G

G

A

A

G

G

K

K

S

T

T

T

L

T

F

L

S

S

L

M

A
x
L

T

T

G

G

L

L

Y

F

G

P

A

P

R

T

A

S

G

G

A

R

V

A

E

Y

I

I

M

S

G

G

Y

Y

D

E

V

I

A

S

K

Q

T

D

P

M

G

V

E

Q

A

I

L
x
R

R

K

R

S

L

L

G

G

V

L

V

C

F

P

Q

Q

A

H

R

D

T

I

L

L

D

F

L

D

E

N

L

L

S

T

L

V

T

A

Q

E

N

H

L

L

L

Y

Y

F

H

Y

A

A

A

Q

L

L

H

K

G

G

I

L

G

S

P

R

G

Q

E

K

G

C

K

P

R

E

L

E

A

V

G

K

A

Q

A

M

L

L

E

H

R

I

A

I

G

G

L

L

S

E

E

D

R

K

A

W

R

N

E

S

K

R

A

S

R

R

D

F

L

L

S

S

G

G

Q

M

M

R

R

R

R

K

V

L

E

S

I

I

A

G

R

I

A

A

L

L

L

I

H

A

R

G

P

S

S

K

L

V

L

L

L

I

L

L

D

D

E

E

P

P

T

T

V
x
S

G

G

L

M

D

D

I

A

K

I

A

S

R

R

A

R

D

A

I

I

L

W

K

D

H

L

V

L

R

Q

G

R

L

-

V

-

A

Q

Q

K

E

S

N

D

L

R

A

T

V

I

L

V

W

L

T

T

H

H

L

F

I

M

D

D

E

E

I

A

-

D

A

L

P

L

S

G

D

D

Q

R

V

I

I

A

M

I

L

M

H

A

E

K

G

G

R

E

V

L

L

Q

T

C

D

C

G

G

S

S

A

S

N568 (= N41) to D: in SMDP3; does not affect location in intracellular vesicle membrane; does not affect proteolytic cleavage; does not affect N-glycosylation; loss of ATP hydrolysis activity; decreases ATP binding in vitro; does not affect protein expression; does not affect multivesicular bodies and lamellar bodies location; affects multivesicular bodies and lamellar bodies development; loss of phosphatidylcholine transport; does not affect cholesterol transport; dbSNP:rs121909184
L579 (≠ A52) to P: in SMDP3; uncertain significance
R605 (≠ L78) to Q: in SMDP3; uncertain significance; dbSNP:rs760006956
S693 (≠ V166) mutation to L: Does not affect protein oligomerization.

Sites not aligning to the query:

43 R → L: in SMDP3; uncertain significance
53 N→Q: Does not affect N-glycosylation. Does not affect protein expression. Does not affect lamellar body membrane location.
101 L → P: in SMDP3; loss of intracellular vesicle membrane location; loss of proteolytic cleavage; does not affect N-glycosylation; loss of ATP hydrolysis activity; decreases ATP binding in vitro; dbSNP:rs121909182
124 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→Q: Loss of N-glycosylation. Reduces protein expression by 50%. Affects anterograde trafficking; when associated with Q-140. Reduces protein expression by 85%; when associated with Q-140. Does not affect lamellar body membrane location.
140 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N → H: in dbSNP:rs45447801; N→Q: Loss of N-glycosylation. Reduces protein expression by 50%. Affects anterograde trafficking; when associated with Q-124. Reduces protein expression by 85%; when associated with Q-140. Does not affect lamellar body membrane location.
173:174 LK→AA: Loss of proteolytic processing.
174:175 Cleavage; by CTSL
215 Q → K: in SMDP3; loss of lamellar bodies membrane location; loss of proteolytic cleavage; increases cellular free cholesterol and phosphatidylcholine transport; loss of vesicles formation; increases free cholesterol induced cell death; loss of protein oligomerization; dbSNP:rs879159551
280 R → C: in SMDP3; uncertain significance; does not affect protein oligomerization; dbSNP:rs201299260
288 R → K: in SMDP3; uncertain significance; does not affect protein oligomerization; dbSNP:rs117603931
290 L → M: in a breast cancer sample; somatic mutation
292 E → V: in SMDP3; uncertain significance; does not affect lamellar bodies membrane location; does not affect proteolytic cleavage; affects lamellar bodies formation; does not affect cholesterol and phosphatidylcholine transport; decreases vesicles formation; does not affect free cholesterol induced cell death; dbSNP:rs149989682
766 P → S: in dbSNP:rs45592239
801 E → D: in a breast cancer sample; somatic mutation
945 N→Q: Does not affect lamellar body membrane location. Does not affect protein expression. Does not affect proteolytic processing.
982 L → P: in SMDP3; loss of intracellular vesicle membrane location; loss of proteolytic cleavage; does not affect N-glycosylation; dbSNP:rs1402761450
1069 H → Q: in a breast cancer sample; somatic mutation
1076 N → K: in SMDP3; uncertain significance; dbSNP:rs2093663770
1221 G → S: in SMDP3; does not affect intracellular vesicle membrane location; does not affect proteolytic cleavage; does not affect N-glycosylation; loss of ATP hydrolysis activity; G→A: Decreases ATP hydrolysis activity of 15% compared to the wild-type.; G→T: Decreases ATP hydrolysis activity of 36% compared to the wild-type.; G→V: Decreases ATP hydrolysis activity of 18% compared to the wild-type.
1302 G → E: in SMDP3; uncertain significance; dbSNP:rs2093657978
1388 K → N: in SMDP3; decreases phosphatidylcholine transport; increases protein abundance; does not affect folding in the endoplasmic reticulum; decreases proteolytic processing; affects lamellar bodies development; reduces free cholesterol transport
1553 L → P: in SMDP3; loss of intracellular vesicle membrane location; loss of proteolytic cleavage; does not affect N-glycosylation; dbSNP:rs121909183
1580 L → P: in SMDP3; does not affect location in intracellular vesicle membrane; does not affect proteolytic cleavage; does not affect N-glycosylation; loss of ATP hydrolysis activity; decreases ATP binding in vitro; affects the intracellular vesicles development; decreases phosphatidylcholine transport; L→A: Decreases ATP hydrolysis activity of 13% compared to the wild-type.; L→F: Decreases ATP hydrolysis activity of 13% compared to the wild-type.; L→V: Decreases ATP hydrolysis activity of 56% compared to the wild-type.
1591 Q → P: in SMDP3; loss of intracellular vesicle membrane location; loss of proteolytic cleavage; does not affect N-glycosylation; dbSNP:rs28936691

1ji0A Crystal structure analysis of the abc transporter from thermotoga maritima
29% identity, 89% coverage: 1:225/254 of query aligns to 1:227/240 of 1ji0A

query
sites
1ji0A

M

M

S

V

E

S

T

D

P

I

A

V

L

L

N

E

V

V

K

Q

S

S

L

L

S

H

H

V

A

Y

Y
|
Y

G

G

A

A

R

I

K

H

A

A

L

I

D

K

D

G

V

I

S

D

L

L

R

K

V

V

E

P

R

R

G

G

A

Q

F

I

A

V

V

T

L

L

L

I

G

G

L

A

N

N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

F

L

S

S

L

A

A

I

T

A

G

G

L

L

Y

V

G

R

A

A

R

Q

A

K

G

G

A

K

V

I

E

I

I

F

M

N

G

G

Y

Q

D

D

V

I

A

T

K

N

T

K

P

P

G

A

E

H

A

V

L

I

R

N

R

R

L

M

G

G

V

I

-

A

-

L

V

V

F

P

Q
x
E

A

G

R

R

T

R

L

I

D

F

L

P

E

E

L

L

S

T

L

V

T

Y

Q

E

N

N

L

L

L

M

Y

M

H

G

A

A

A

Y

L

N

H

R

G

K

I

D

G

K

P

E

G

G

E

I

G

K

K

R

R

D

L

L

A

E

G

W

A

I

A

F

L

S

E

L

R

F

A

P

G

R

L

L

S

K

E

E

R

R

A

L

R

K

E

Q

K

L

A

G

R

G

D

T

L

L

S

S

G

G

Q

E

M

Q

R

Q

R

M

V

L

E

A

I

I

A

G

R

R

A

A

L

L

L

M

H

S

R

R

P

P

S

K

L

L

L

M

D

D

E
|
E

P

P

T

S

V

L

G

G

L

L

D

A

I

P

K

I

A

L

R

V

A

S

D

E

I

V

L

F

K

E

H

V

V

I

R

Q

G

K

L

I

V

N

A

Q

Q

E

E

G

N

T

L

T

A

I

V

L

L

L

W

V

T

E

T

Q

H

N

L

A

I

L

D

G

E

A

I

L

A

K

P

V

S

A

D

H

Q

Y

V

G

I

Y

M

V

L

L

H

E

E

T

G

G

R

Q

V

I

L

V

T

L

D

E

G

G

S

K

A

A

A

S

E

E

I

L

V

L

binding adenosine-5'-triphosphate: Y16 (= Y16), G42 (= G42), G44 (= G44), K45 (= K45), T46 (≠ S46), T47 (= T47), E88 (≠ Q86), E165 (= E163)

8wm7C Cryo-em structure of cyanobacterial nitrate/nitrite transporter nrtbcd in complex with signalling protein pii (see paper)
34% identity, 76% coverage: 21:213/254 of query aligns to 22:213/658 of 8wm7C

query
sites
8wm7C

A

A

L

L

D

K

D

N

V

I

S

E

L

L

R

K

V

I

E

K

R

Q

G

G

A

E

F

F

A

V

V

S

L

L

L

I

G

G

L

H

N

S

G
|
G

A
x
C

G
|
G

K

K

S
|
S

T

T

L

L

F

L

S

N

L

I

A

I

T

A

G

G

L

L

Y

D

G

R

A

A

R

S

A

I

G

G

A

G

V

V

E

T

I

L

M

E

G

G

Y

R

D

E

V

I

A

R

K

E

T

P

P

S

G

P

E

D

A

R

L

M

R

-

L

-

G

-

V

V

F

F

Q

Q

A

N

R

Y

T

S

L

L

D

L

L

P

E

W

L

L

S

T

L

V

T

R

Q

E

N

N

-

V

-

A

L

L

L

A

Y

V

H

D

A

E

A

V

L

Y

H

Q

G

G

I

K

G

S

P

K

G

G

E

E

G

R

K

R

R

A

L

I

A

I

G

E

A

E

A

H

L

I

E

D

R

M

A

V

G

G

L

L

S

R

E

L

R

A

A

A

R

N

E

K

K

R

A

P

R

S

D

E

L

L

S

S

G

G

Q

M

M

K

R

Q

R

R

V

V

E

A

I

I

A

A

R

R

A

A

L

L

L

A

H

T

R

R

P

P

S

K

L

L

D

D

E

E

P

P

T

F

V

G

G

A

L

L

D

D

I

A

K

L

A

T

R

R

A

G

D

S

I

L

L

Q

K

E

H

Q

V

L

R

M

G

K

L

I

V

C

A

N

Q

E

E

H

N

Q

L

I

A

T

V

C

L

V

W

M

T

V

T

T

H

H

L

D

I

V

D

D

E

E

-

A

I

L

A

L

P

L

S

S

D

D

Q

R

V

V

I

V

M

M

L

L

H

T

E

N

G

G

binding adenosine-5'-diphosphate: G43 (= G42), C44 (≠ A43), G45 (= G44), S47 (= S46)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12, 20

6mjpA Lptb(e163q)fgc from vibrio cholerae (see paper)
31% identity, 89% coverage: 7:233/254 of query aligns to 3:232/240 of 6mjpA

query
sites
6mjpA

L

L

N

K

V

A

K

Q

S

H

L

L

S

A

H

K

A

S

Y

Y

G

K

A

K

R

R

K

K

A

V

L

V

D

S

D

D

V

V

S

S

L

L

R

Q

V

V

E

E

R

S

G

G

A

Q

F

I

A

V

V

G

L

L

G

G

L

P

N

N

G

G

A

A

G

G

K

K

S

T

T

T

L

S

F

F

S

Y

L

M

A

I

T

V

G

G

L

L

Y

V

G

A

A

R

R

D

A

E

G

G

A

T

V

I

E

T

I

I

M

D

G

D

Y

N

D

D

V

I

A

S

K

I

T

L

P

P

G

M

E

H

A

S

L

R

R

S

R

R

L

M

G

G

V

I

V

G

F

Y

-

L

-

P

Q

Q

A

E

R

A

T

S

L

I

D

F

L

R

E

K

L

L

S

S

L

V

T

E

Q

D

N

N

L

I

L

M

Y

-

H

-

A

A

A

V

L

L

H

Q

G

T

-

R

-

E

I

L

G

T

P

H

G
x
E

E

E

G

R

K

Q

R
x
D

L

K

A

L

G

E

A

D

A

L

L

L

E

E

R

E

A

F

G

H

L

I

S

Q

E

H

R

I

A

R

R

K

E

S

K

A

A

G

R

M

D

A

L

L

S

S

G

G

Q

E

M

R

R

R

V

V

E

E

I

I

A

A

R

R

A

A

L

L

L

A

H

A

R

N

P

P

S

Q

L

F

L

I

L

L

D

D

E

Q

P

P

T

F

V

A

G

G

L

V

D

D

I

P

K

I

A

S

R

V

A

I

D

D

I

I

L

K

K

K

H

I

V

I

R

E

G

H

L

L

V

-

A

R

Q

D

E

R

N

G

L

L

A

G

V

V

L

L

W

I

T

T

T

D

H

H

L

N

I

V

D

R

E

E

-

T

I

L

A

D

P

V

S

C

D

E

Q

K

V

A

I

Y

M

I

L

V

H

S

E

Q

G

G

R

R

V

L

L

I

T

A

D

E

G

G

S

T

A

P

A

Q

E

D

I

V

V

L

A

N

Q

E

E

Q

T

V

D

K

S

Q

I

V

binding calcium ion: E111 (≠ G112), D115 (≠ R116)

7w79A Heme exporter hrtba in complex with mn-amppnp (see paper)
33% identity, 83% coverage: 5:216/254 of query aligns to 3:216/216 of 7w79A

query
sites
7w79A

P

P

A

V

L

L

N
x
S

V

I

K

T

S

N

L

A

S

S

H

V

A

V

Y
|
Y

-

P

-

D

-

G

-

I

G

S

A

T

R

V

K

T

A

A

L

L

D

D

D

S

V

A

S

N

L

V

R
x
E

V

I

E

F

R

P

G

G

A

E

F

L

A

V

V

A

L

I

L

V

G

G

L

E

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

F

L

S

S

L

I

A

A

T

G

G

F

L

L

Y

Q

G

E

A

P

R

T

A

S

G

G

A

T

V

V

E

T

I

L

M

H

G

G

Y

A

D

E

V

G

A

L

K

D

T

A

P

T

G

S

E

T

A

R

L

R

R

E

R

H

L

I

G

G

V

F

V

V

F

F

Q
|
Q

A

Q

R

P

T

N

L

L

D

L

L

G

E

S

L

L

S

T

L

A

T

R

Q

E

N

Q

L

L

Y

I

H

T

A

D

A

H

L

L

H

R

G

G

I

I

G

K

P

P

G

R

E

K

G

D

K

R

R

-

L

-

A

A

G

D

A

E

A

L

L

L

E

A

R

R

A

V

G

G

L

L

S

K

E

G

R

L

A

G

R

G

E

R

K

R

A

V

R

A

D

Q

L

L

S

S

G

G

Q

Q

M

R

R

Q

R

R

V

V

E

N

I

I

A

A

R

R

A

A

L

L

L

M

H

G

R

N

P

P

S

Q

L

L

L

A

D

D

E
|
E

P

P

T

T

V

S

G

A

L

L

D

D

I

A

K

R

A

L

R

S

A

K

D

E

I

I

L

V

K

E

H

L

V

L

R

R

G

D

L

V

V

T

A

K

Q

E

E

F

N

A

L

L

A

A

V

T

L

L

W

M

T

V

T

T

H
|
H

L

D

I

R

D

S

E

Q

I

L

A

A

P

Y

S

A

D

D

Q

R

V

F

I

V

M

E

L

M

H

A

E

D

G

G

R

K

V

A

L

L

binding adenosine-5'-triphosphate: Y14 (= Y16), S43 (≠ N41), G44 (= G42), S45 (≠ A43), G46 (= G44), K47 (= K45), S48 (= S46), T49 (= T47)
binding manganese (ii) ion: S6 (≠ N8), E30 (≠ R28), S48 (= S46), Q88 (= Q86), E163 (= E163), H196 (= H196)

Query Sequence

>WP_085772469.1 NCBI__GCF_002117405.1:WP_085772469.1
MSETPALNVKSLSHAYGARKALDDVSLRVERGAFAVLLGLNGAGKSTLFSLATGLYGARA
GAVEIMGYDVAKTPGEALRRLGVVFQARTLDLELSLTQNLLYHAALHGIGPGEGKRLAGA
ALERAGLSERAREKARDLSGGQMRRVEIARALLHRPSLLLLDEPTVGLDIKARADILKHV
RGLVAQENLAVLWTTHLIDEIAPSDQVIMLHEGRVLTDGSAAEIVAAQETDSIGGAFEKL
TMGVVPSPRERGEG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory