SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_086508157.1 NCBI__GCF_002151265.1:WP_086508157.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

5az7A Crystal structure of mbp-tom20 fusion protein with a 4-residue spacer in the connector helix (see paper)
50% identity, 92% coverage: 31:395/395 of query aligns to 6:370/434 of 5az7A

query
sites
5az7A

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binding alpha-D-glucopyranose: D14 (≠ N39), K15 (= K40), W62 (= W87), D65 (= D90), R66 (= R91), E111 (= E136), E153 (= E178), P154 (= P179), Y155 (= Y180), Y155 (= Y180), W340 (= W365)

3csbA Crystal structure of monobody ysx1/maltose binding protein fusion complex (see paper)
50% identity, 92% coverage: 31:395/395 of query aligns to 3:367/464 of 3csbA

query
sites
3csbA

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binding manganese (ii) ion: D284 (≠ E312), E307 (= E335)

1llsA Crystal structure of unliganded maltose binding protein with xenon (see paper)
50% identity, 92% coverage: 31:395/395 of query aligns to 6:370/370 of 1llsA

query
sites
1llsA

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binding xenon: F61 (≠ I86), V293 (≠ F318)

1ez9B Structure of maltotetraitol bound to open-form maltodextrin binding protein in p1 crystal form (see paper)
50% identity, 92% coverage: 31:395/395 of query aligns to 6:370/370 of 1ez9B

query
sites
1ez9B

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D

K

D

D

I

V

G

G

V

V

N

D

N

N

E

A

G

G

A

A

L

K

Q

A

G

G

A

L

E

T

L

F

L

L

V

V

E

D

L

L

I

I

E

K

S

N

G

K

V

H

L

M

P

N

R

A

G

D

T

T

D

D

Y
|
Y

S

S

I

I

M

A

D

E

T

A

R

A

F

F

N

N

R

K

G

G

E

E

V

T

A

A

A

M

M

T

I

I

S

N

G

G

P

P

W
|
W

A

A

W

W

S

S

N

N

L

I

E

D

Q

T

S

S

G

K

I

V

D

N

Y

Y

G

G

V

V

A

T

L

V

L

L

P

P

K

T

V

F

G

K

E

G

E

Q

R

P

A

S

K

K

P

P

M

F

F

V

G

G

V

V

M

L

A

S

A

A

M

G

I

I

N

N

T

A

A

A

S

S

P

P

N

N

D

K

F

E

L

L

A

A

V

K

E

E

F

F

L

L

E

E

N

N

Y

Y

L

L

L

L

S

T

E

D

E

E

G

G

M

L

R

E

T

A

F

V

N

N

S

K

D

D

S

K

T

P

L

L

G

G

A

A

V

V

A

A

H

L

I

K

A

S

Y

Y

Q

E

Q

E

E

E

L

L

E

A

S

K

D

D

P

P

N

R

I

I

A

A

A

A

T

T

L

M

E

E

N

N

A

A

E

Q

L

K

G

G

M

E

P

I

M

M

P

P

N

N

I

I

P

P

E

Q

M

M

G

S

A

A

F

F

W
|
W

A
x
Y

A

A

M

V

E
x
R

P

T

A

A

L

V

Q

I

N

N

I

A

G

A

S

S

G

G

R

R

Q

Q

S

T

P

V

R

D

E

E

A

A

L

L

D

K

A

D

A

A

A

Q

R

T

R

R

M

I

R

T

Q

K

binding alpha-D-glucopyranose: E111 (= E136), E153 (= E178), E153 (= E178), P154 (= P179), Y155 (= Y180), Y155 (= Y180), F156 (≠ Y181), W230 (= W255), W340 (= W365), W340 (= W365), Y341 (≠ A366), R344 (≠ E369)
binding D-glucose: F156 (≠ Y181), Y210 (= Y235), W230 (= W255)

6k7fA Crystal structure of mbpholo-tim21 fusion protein with a 17-residue helical linker (see paper)
50% identity, 92% coverage: 31:393/395 of query aligns to 7:369/499 of 6k7fA

query
sites
6k7fA

R

K

L

L

T

V

I

I

W

W

M

I

G

N

D

G

N
x
D

K
|
K

G

G

Y

Y

D

N

G

G

I

L

R

A

E

E

V

V

A

G

R

K

Q

K

F

F

A

E

D

K

D

D

T

T

G

G

I

I

E

K

V

V

R

T

I

V

V

E

N

H

P

P

D

D

N

K

L

L

T

E

D

E

R

K

F

F

Q

P

Q

Q

A

V

A

A

G

A

S

T

G

G

Q

D

G

G

P

P

D

D

I

I

V

I

I

F

W
|
W

A

A

H

H

D
|
D

R
|
R

I

F

G

G

E

G

W

Y

A

A

Q

Q

S

S

G

G

L

L

L

L

A

A

P

E

V

I

S

T

P

P

S

D

S

K

D

A

F

F

R

Q

E

D

R

K

Y

L

F

Y

D

P

F

F

T

T

W

W

D

D

A

A

T

V

L

R

W

Y

S

N

G

G

E

K

H

L

Y

I

G

A

Y

Y

P

P

I

I

S

A

V

V

E
|
E

A

A

L

L

G

S

L

L

I

I

Y

Y

N

N

K

K

A

D

L

L

V

L

E

P

T

N

P

P

P

P

E

K

S

T

F

W

A

E

E

E

L

I

A

P

Q

A

L

L

D

D

A

K

E

E

L

L

A

K

E

A

Q

K

G

G

R

K

K

S

A

A

I

L

L

M

F

F

D

N

Y

L

G

Q

E
|
E

P
|
P

Y
|
Y

Y

F

G

T

W

W

T

P

L

L

L

I

A

A

A

A

N

D

G

G

G

G

Y

Y

P

A

F

F

R

K

R

Y

T

E

E

N

E

G

G

K

F

Y

D

D

V

I

D

K

D

D

I

V

G

G

V

V

N

D

N

N

E

A

G

G

A

A

L

K

Q

A

G

G

A

L

E

T

L

F

L

L

V

V

E

D

L

L

I

I

E

K

S

N

G

K

V

H

L

M

P

N

R

A

G

D

T

T

D

D

Y

Y

S

S

I

I

M

A

D

E

T

A

R

A

F

F

N

N

R

K

G

G

E

E

V

T

A

A

A

M

M

T

I

I

S

N

G

G

P

P

W
|
W

A

A

W

W

S

S

N

N

L

I

E

D

Q

T

S

S

G

K

I

V

D

N

Y

Y

G

G

V

V

A

T

L

V

L

L

P

P

K

T

V

F

G

K

E

G

E

Q

R

P

A

S

K

K

P

P

M

F

F

V

G

G

V

V

M

L

A

S

A

A

M

G

I

I

N

N

T

A

A

A

S

S

P

P

N

N

D

K

F

E

L

L

A

A

V

K

E

E

F

F

L

L

E

E

N

N

Y

Y

L

L

L

L

S

T

E

D

E

E

G

G

M

L

R

E

T

A

F

V

N

N

S

K

D

D

S

K

T

P

L

L

G

G

A

A

V

V

A

A

H

L

I

K

A

S

Y

Y

Q

E

Q

E

E

E

L

L

E

V

S

K

D

D

P

P

N

R

I

I

A

A

A

A

T

T

L

M

E

E

N

N

A

A

E

Q

L

K

G

G

M

E

P

I

M

M

P

P

N

N

I

I

P

P

E

Q

M

M

G

S

A

A

F

F

W
|
W

A

Y

A

A

M

V

E

R

P

T

A

A

L

V

Q

I

N

N

I

A

G

A

S

S

G

G

R

R

Q

Q

S

T

P

V

R

D

E

E

A

A

L

L

D

K

A

D

A

A

A

Q

R

T

R

R

M

I

binding alpha-D-glucopyranose: D15 (≠ N39), K16 (= K40), W63 (= W87), D66 (= D90), R67 (= R91), E112 (= E136), E154 (= E178), P155 (= P179), Y156 (= Y180), Y156 (= Y180), W231 (= W255), W341 (= W365)

3woaA Crystal structure of lambda repressor (1-45) fused with maltose- binding protein (see paper)
49% identity, 95% coverage: 16:389/395 of query aligns to 38:411/413 of 3woaA

query
sites
3woaA

L

M

G

G

S

M

G

G

L

Q

A

S

F

G

S

S

T

G

Y

M

A

K

F

I

D

E

G

E

D

G

R

K

L

L

T

V

I

I

W

W

M

I

G

N

D

G

N
x
D

K
|
K

G

G

Y

Y

D

N

G

G

I

L

R

A

E

E

V

V

A

G

R

K

Q

K

F

F

A

E

D

K

D

D

T

T

G

G

I

I

E

K

V

V

R

T

I

V

V

E

N

H

P

P

D

D

N

K

L

L

T

E

D

E

R

K

F

F

Q

P

Q

Q

A

V

A

A

G

A

S

T

G

G

Q

D

G

G

P

P

D

D

I

I

V

I

I

F

W
|
W

A

A

H

H

D
|
D

R
|
R

I

F

G

G

E

G

W

Y

A

A

Q

Q

S

S

G

G

L

L

L

L

A

A

P

E

V

I

S

T

P

P

S

D

S

K

D

A

F

F

R

Q

E

D

R

K

Y

L

F

Y

D

P

F

F

T

T

W

W

D

D

A

A

T

V

L

R

W

Y

S

N

G

G

E

K

H

L

Y

I

G

A

Y

Y

P

P

I

I

S

A

V

V

E
|
E

A

A

L

L

G

S

L

L

I

I

Y

Y

N

N

K

K

A

D

L

L

V

L

E

P

T

N

P

P

P

P

E

K

S

T

F

W

A

E

E

E

L

I

A

P

Q

A

L

L

D

D

A

K

E

E

L

L

A

K

E

A

Q

K

G

G

R

K

K

S

A

A

I

L

L

M

F

F

D

N

Y

L

G

Q

E
|
E

P
|
P

Y
|
Y

Y

F

G

T

W

W

T

P

L

L

L

I

A

A

A

A

N

D

G

G

G

G

Y

Y

P

A

F

F

R

K

R

Y

T

E

E

N

E

G

G

K

F

Y

D

D

V

I

D

K

D

D

I

V

G

G

V

V

N

D

N

N

E

A

G

G

A

A

L

K

Q

A

G

G

A

L

E

T

L

F

L

L

V

V

E

D

L

L

I

I

E

K

S

N

G

K

V

H

L

M

P

N

R

A

G

D

T

T

D

D

Y

Y

S

S

I

I

M

A

D

E

T

A

R

A

F

F

N

N

R

K

G

G

E

E

V

T

A

A

A

M

M

T

I

I

S

N

G

G

P

P

W
|
W

A

A

W

W

S

S

N

N

L

I

E

D

Q

T

S

S

G

K

I

V

D

N

Y

Y

G

G

V

V

A

T

L

V

L

L

P

P

K

T

V

F

G

K

E

G

E

Q

R

P

A

S

K

K

P

P

M

F

F

V

G

G

V

V

M

L

A

S

A

A

M

G

I

I

N

N

T

A

A

A

S

S

P

P

N

N

D

K

F

E

L

L

A

A

V

K

E

E

F

F

L

L

E

E

N

N

Y

Y

L

L

L

L

S

T

E

D

E

E

G

G

M

L

R

E

T

A

F

V

N

N

S

K

D

D

S

K

T

P

L

L

G

G

A

A

V

V

A

A

H

L

I

K

A

S

Y

Y

Q

E

Q

E

E

E

L

L

E

A

S

K

D

D

P

P

N

R

I

I

A

A

A

A

T

T

L

M

E

E

N

N

A

A

E

Q

L

K

G

G

M

E

P

I

M

M

P

P

N

N

I

I

P

P

E

Q

M

M

G

S

A

A

F

F

W
|
W

A

Y

A

A

M

V

E

R

P

T

A

A

L

V

Q

I

N

N

I

A

G

A

S

S

G

G

R

R

Q

Q

S

T

P

V

R

D

E

E

A

A

L

L

D

K

A

D

A

A

binding alpha-D-glucopyranose: D61 (≠ N39), K62 (= K40), W109 (= W87), D112 (= D90), R113 (= R91), E158 (= E136), E200 (= E178), P201 (= P179), Y202 (= Y180), Y202 (= Y180), W277 (= W255), W387 (= W365)

6m4wA Crystal structure of mbp fused split fkbp-frb t2098l mutant in complex with rapamycin (see paper)
50% identity, 91% coverage: 31:389/395 of query aligns to 7:365/403 of 6m4wA

query
sites
6m4wA

R

K

L

L

T

V

I

I

W

W

M

I

G

N

D

G

N
x
D

K
|
K

G

G

Y

Y

D

N

G

G

I

L

R

A

E

E

V

V

A

G

R

K

Q

K

F

F

A

E

D

K

D

D

T

T

G

G

I

I

E

K

V

V

R

T

I

V

V

E

N

H

P

P

D

D

N

K

L

L

T

E

D

E

R

K

F

F

Q

P

Q

Q

A

V

A

A

G

A

S

T

G

G

Q

D

G

G

P

P

D

D

I

I

V

I

I

F

W
|
W

A

A

H

H

D
|
D

R

R

I

F

G

G

E

G

W

Y

A

A

Q

Q

S

S

G

G

L

L

L

L

A

A

P

E

V

I

S

T

P

P

S

A

S

A

D

A

F

F

R

Q

E

D

R

K

Y

L

F

Y

D

P

F

F

T

T

W

W

D

D

A

A

T

V

L

R

W

Y

S

N

G

G

E

K

H

L

Y

I

G

A

Y

Y

P

P

I

I

S

A

V

V

E
|
E

A

A

L

L

G

S

L

L

I

I

Y

Y

N

N

K

K

A

D

L

L

V

L

E

P

T

N

P

P

P

P

E

K

S

T

F

W

A

E

E

E

L

I

A

P

Q

A

L

L

D

D

A

K

E

E

L

L

A

K

E

A

Q

K

G

G

R

K

K

S

A

A

I

L

L

M

F

F

D

N

Y

L

G

Q

E
|
E

P
|
P

Y
|
Y

Y

F

G

T

W

W

T

P

L

L

L

I

A

A

A

A

N

D

G

G

G

G

Y

Y

P

A

F

F

R

K

R

Y

T

A

E
x
A

E

G

G
x
K

F

Y

D

D

V

I

D

K

D

D

I

V

G

G

V

V

N

D

N

N

E

A

G

G

A

A

L

K

Q

A

G

G

A

L

E

T

L

F

L

L

V

V

E

D

L

L

I

I

E

K

S

N

G

K

V

H

L

M

P

N

R

A

G

D

T

T

D

D

Y

Y

S

S

I

I

M

A

D

E

T

A

R

A

F

F

N

N

R

K

G

G

E

E

V

T

A

A

A

M

M

T

I

I

S

N

G

G

P

P

W
|
W

A

A

W

W

S

S

N

N

L

I

E

D

Q

T

S

S

G

A

I

V

D

N

Y

Y

G

G

V

V

A

T

L

V

L

L

P

P

K

T

V

F

G

K

E

G

E

Q

R

P

A

S

K

K

P

P

M

F

F

V

G

G

V

V

M

L

A

S

A

A

M

G

I

I

N

N

T

A

A

A

S

S

P

P

N

N

D

K

F

E

L

L

A

A

V

K

E

E

F

F

L

L

E

E

N

N

Y

Y

L

L

L

L

S

T

E

D

E

E

G

G

M

L

R

E

T

A

F

V

N

N

S

K

D

D

S

K

T

P

L

L

G

G

A

A

V

V

A

A

H

L

I

K

A

S

Y

Y

Q

E

Q

E

E

E

L

L

E

A

S

K

D

D

P

P

N

R

I

I

A

A

A

A

T

T

L

M

E

E

N

N

A

A

E

Q

L

K

G

G

M

E

P

I

M

M

P

P

N

N

I

I

P

P

E

Q

M

M

G

S

A

A

F

F

W
|
W

A

Y

A

A

M

V

E

R

P

T

A

A

L

V

Q

I

N

N

I

A

G

A

S

S

G

G

R

R

Q

Q

S

T

P

V

R

D

E

E

A

A

L

L

D

K

A

D

A

A

binding alpha-D-glucopyranose: D15 (≠ N39), K16 (= K40), W63 (= W87), D66 (= D90), E112 (= E136), E154 (= E178), P155 (= P179), Y156 (= Y180), Y156 (= Y180), W231 (= W255), W341 (= W365)
binding rapamycin immunosuppressant drug: A174 (≠ E198), K176 (≠ G200)

5y2gA Structure of mbp tagged gbs camp (see paper)
50% identity, 89% coverage: 31:381/395 of query aligns to 4:354/581 of 5y2gA

query
sites
5y2gA

R

K

L

L

T

V

I

I

W

W

M

I

G

N

D

G

N

D

K
|
K

G

G

Y

Y

D

N

G

G

I

L

R

A

E

E

V

V

A

G

R

K

Q

K

F

F

A

E

D

K

D

D

T

T

G

G

I

I

E

K

V

V

R

T

I

V

V

E

N

H

P

P

D

D

N

K

L

L

T

E

D

E

R

K

F

F

Q

P

Q

Q

A

V

A

A

G

A

S

T

G

G

Q

D

G

G

P

P

D

D

I

I

V

I

I

F

W

W

A

A

H

H

D
|
D

R
|
R

I

F

G

G

E

G

W

Y

A

A

Q

Q

S

S

G

G

L

L

L

L

A

A

P

E

V

I

S

T

P

P

S

A

S

A

D

A

F

F

R

Q

E

D

R

K

Y

L

F

Y

D

P

F

F

T

T

W

W

D

D

A

A

T

V

L

R

W

Y

S

N

G

G

E

K

H

L

Y

I

G

A

Y

Y

P

P

I

I

S

A

V

V

E
|
E

A

A

L

L

G

S

L

L

I

I

Y

Y

N

N

K

K

A

D

L

L

V

L

E

P

T

N

P

P

P

P

E

K

S

T

F

W

A

E

E

E

L

I

A

P

Q

A

L

L

D

D

A

K

E

E

L

L

A

K

E

A

Q

K

G

G

R

K

K

S

A

A

I

L

L

M

F

F

D

N

Y

L

G

Q

E
|
E

P
|
P

Y
|
Y

Y

F

G

T

W

W

T

P

L

L

L

I

A

A

A

A

N

D

G

G

G

G

Y

Y

P

A

F

F

R

K

R

Y

T

A

E

A

E

G

G

K

F

Y

D

D

V

I

D

K

D

D

I

V

G

G

V

V

N

D

N

N

E

A

G

G

A

A

L

K

Q

A

G

G

A

L

E

T

L

F

L

L

V

V

E

D

L

L

I

I

E

K

S

N

G

K

V

H

L

M

P

N

R

A

G

D

T

T

D

D

Y

Y

S

S

I

I

M

A

D

E

T

A

R

A

F

F

N

N

R

K

G

G

E

E

V

T

A

A

A

M

M

T

I

I

S

N

G

G

P

P

W
|
W

A

A

W

W

S

S

N

N

L

I

E

D

Q

T

S

S

G

A

I

V

D

N

Y

Y

G

G

V

V

A

T

L

V

L

L

P

P

K

T

V

F

G

K

E

G

E

Q

R

P

A

S

K

K

P

P

M

F

F

V

G

G

V

V

M

L

A

S

A

A

M

G

I

I

N

N

T

A

A

A

S

S

P

P

N

N

D

K

F

E

L

L

A

A

V

K

E

E

F

F

L

L

E

E

N

N

Y

Y

L

L

L

L

S

T

E

D

E

E

G

G

M

L

R

E

T

A

F

V

N

N

S

K

D

D

S

K

T

P

L

L

G

G

A

A

V

V

A

A

H

L

I

K

A

S

Y

Y

Q

E

Q

E

E

E

L

L

E

A

S

K

D

D

P

P

N

R

I

I

A

A

A

A

T

T

L

M

E

E

N

N

A

A

E

Q

L

K

G

G

M

E

P

I

M

M

P

P

N

N

I

I

P

P

E

Q

M

M

G

S

A

A

F

F

W
|
W

A

Y

A

A

M

V

E

R

P

T

A

A

L

V

Q

I

N

N

I

A

G

A

S

S

G

G

R

R

Q

Q

S

T

binding alpha-D-glucopyranose: K13 (= K40), D63 (= D90), R64 (= R91), E109 (= E136), E151 (= E178), P152 (= P179), Y153 (= Y180), W228 (= W255), W338 (= W365)

7wr3A Crystal structure of mbp-fused ospc3 in complex with calmodulin (see paper)
50% identity, 89% coverage: 31:381/395 of query aligns to 6:356/794 of 7wr3A

query
sites
7wr3A

R

K

L

L

T

V

I

I

W

W

M

I

G

N

D

G

N
x
D

K
|
K

G

G

Y

Y

D

N

G

G

I

L

R

A

E

E

V

V

A

G

R

K

Q

K

F

F

A

E

D

K

D

D

T

T

G

G

I

I

E

K

V

V

R

T

I

V

V

E

N

H

P

P

D

D

N

K

L

L

T

E

D

E

R

K

F

F

Q

P

Q

Q

A

V

A

A

G

A

S

T

G

G

Q

D

G

G

P

P

D

D

I

I

V

I

I

F

W
|
W

A

A

H

H

D
|
D

R
|
R

I

F

G

G

E

G

W

Y

A

A

Q

Q

S

S

G

G

L

L

L

L

A

A

P

E

V

I

S

T

P

P

S

A

S

A

D

A

F

F

R

Q

E

D

R

K

Y

L

F

Y

D

P

F

F

T

T

W

W

D

D

A

A

T

V

L

R

W

Y

S

N

G

G

E

K

H

L

Y

I

G

A

Y

Y

P

P

I

I

S

A

V

V

E
|
E

A

A

L

L

G

S

L

L

I

I

Y

Y

N

N

K

K

A

D

L

L

V

L

E

P

T

N

P

P

P

P

E

K

S

T

F

W

A

E

E

E

L

I

A

P

Q

A

L

L

D

D

A

K

E

E

L

L

A

K

E

A

Q

K

G

G

R

K

K

S

A

A

I

L

L

M

F

F

D

N

Y

L

G

Q

E
|
E

P
|
P

Y
|
Y

Y

F

G

T

W

W

T

P

L

L

L

I

A

A

A

A

N

D

G

G

G

G

Y

Y

P

A

F

F

R

K

R

Y

T

A

E

A

E

G

G

K

F

Y

D

D

V

I

D

K

D

D

I

V

G

G

V

V

N

D

N

N

E

A

G

G

A

A

L

K

Q

A

G

G

A

L

E

T

L

F

L

L

V

V

E

D

L

L

I

I

E

K

S

N

G

K

V

H

L

M

P

N

R

A

G

D

T

T

D

D

Y

Y

S

S

I

I

M

A

D

E

T

A

R

A

F

F

N

N

R

K

G

G

E

E

V

T

A

A

A

M

M

T

I

I

S

N

G

G

P

P

W
|
W

A

A

W

W

S

S

N

N

L

I

E

D

Q

T

S

S

G

A

I

V

D

N

Y

Y

G

G

V

V

A

T

L

V

L

L

P

P

K

T

V

F

G

K

E

G

E

Q

R

P

A

S

K

K

P

P

M

F

F

V

G

G

V

V

M

L

A

S

A

A

M

G

I

I

N

N

T

A

A

A

S

S

P

P

N

N

D

K

F

E

L

L

A

A

V

K

E

E

F

F

L

L

E

E

N

N

Y

Y

L

L

L

L

S

T

E

D

E

E

G

G

M

L

R

E

T

A

F

V

N

N

S

K

D

D

S

K

T

P

L

L

G

G

A

A

V

V

A

A

H

L

I

K

A

S

Y

Y

Q

E

Q

E

E

E

L

L

E

A

S

K

D

D

P

P

N

R

I

I

A

A

A

A

T

T

L

M

E

E

N

N

A

A

E

Q

L

K

G

G

M

E

P

I

M

M

P

P

N

N

I

I

P

P

E

Q

M

M

G

S

A

A

F

F

W
|
W

A

Y

A

A

M

V

E

R

P

T

A

A

L

V

Q

I

N

N

I

A

G

A

S

S

G

G

R

R

Q

Q

S

T

binding alpha-D-glucopyranose: D14 (≠ N39), K15 (= K40), W62 (= W87), D65 (= D90), R66 (= R91), E111 (= E136), E153 (= E178), P154 (= P179), Y155 (= Y180), Y155 (= Y180), W230 (= W255), W340 (= W365)

Sites not aligning to the query:

binding nicotinamide: 462, 463, 499, 500, 501, 517, 522

5h7qA Crystal structure of human mnda pyd domain with mbp tag (see paper)
50% identity, 89% coverage: 31:381/395 of query aligns to 6:356/469 of 5h7qA

query
sites
5h7qA

R

K

L

L

T

V

I

I

W

W

M

I

G

N

D

G

N
x
D

K
|
K

G

G

Y

Y

D

N

G

G

I

L

R

A

E

E

V

V

A

G

R

K

Q

K

F

F

A

E

D

K

D

D

T

T

G

G

I

I

E

K

V

V

R

T

I

V

V

E

N

H

P

P

D

D

N

K

L

L

T

E

D

E

R

K

F

F

Q

P

Q

Q

A

V

A

A

G

A

S

T

G

G

Q

D

G

G

P

P

D

D

I

I

V

I

I

F

W
|
W

A

A

H

H

D
|
D

R
|
R

I

F

G

G

E

G

W

Y

A

A

Q

Q

S

S

G

G

L

L

L

L

A

A

P

E

V

I

S

T

P

P

S

A

S

A

D

A

F

F

R

Q

E

D

R

K

Y

L

F

Y

D

P

F

F

T

T

W

W

D

D

A

A

T

V

L

R

W

Y

S

N

G

G

E

K

H

L

Y

I

G

A

Y

Y

P

P

I

I

S

A

V

V

E
|
E

A

A

L

L

G

S

L

L

I

I

Y

Y

N

N

K

K

A

D

L

L

V

L

E

P

T

N

P

P

P

P

E

K

S

T

F

W

A

E

E

E

L

I

A

P

Q

A

L

L

D

D

A

K

E

E

L

L

A

K

E

A

Q

K

G

G

R

K

K

S

A

A

I

L

L

M

F

F

D

N

Y

L

G

Q

E
|
E

P
|
P

Y
|
Y

Y
x
F

G

T

W

W

T

P

L

L

L

I

A

A

A

A

N

D

G

G

G

G

Y

Y

P

A

F

F

R

K

R

Y

T

A

E

A

E

G

G

K

F

Y

D

D

V

I

D

K

D

D

I

V

G

G

V

V

N

D

N

N

E

A

G

G

A

A

L

K

Q

A

G

G

A

L

E

T

L

F

L

L

V

V

E

D

L

L

I

I

E

K

S

N

G

K

V

H

L

M

P

N

R

A

G

D

T

T

D

D

Y

Y

S

S

I

I

M

A

D

E

T

A

R

A

F

F

N

N

R

K

G

G

E

E

V

T

A

A

A

M

M

T

I

I

S

N

G

G

P

P

W
|
W

A

A

W

W

S

S

N

N

L

I

E

D

Q

T

S

S

G

A

I

V

D

N

Y

Y

G

G

V

V

A

T

L

V

L

L

P

P

K

T

V

F

G

K

E

G

E

Q

R

P

A

S

K

K

P

P

M

F

F

V

G

G

V

V

M

L

A

S

A

A

M

G

I

I

N

N

T

A

A

A

S

S

P

P

N

N

D

K

F

E

L

L

A

A

V

K

E

E

F

F

L

L

E

E

N

N

Y

Y

L

L

L

L

S

T

E

D

E

E

G

G

M

L

R

E

T

A

F

V

N

N

S

K

D

D

S

K

T

P

L

L

G

G

A

A

V

V

A

A

H

L

I

K

A

S

Y

Y

Q

E

Q

E

E

E

L

L

E

A

S

K

D

D

P

P

N

R

I

I

A

A

A

A

T

T

L

M

E

E

N

N

A

A

E

Q

L

K

G

G

M

E

P

I

M

M

P

P

N

N

I

I

P

P

E

Q

M

M

G

S

A

A

F

F

W
|
W

A

Y

A

A

M

V

E

R

P

T

A

A

L

V

Q

I

N

N

I

A

G

A

S

S

G

G

R

R

Q

Q

S

T

binding alpha-D-glucopyranose: D14 (≠ N39), K15 (= K40), W62 (= W87), D65 (= D90), R66 (= R91), E111 (= E136), E153 (= E178), P154 (= P179), Y155 (= Y180), Y155 (= Y180), F156 (≠ Y181), W230 (= W255), W340 (= W365)

7cy5A Crystal structure of cmd1 in complex with vitamin c (see paper)
50% identity, 89% coverage: 31:381/395 of query aligns to 3:353/862 of 7cy5A

query
sites
7cy5A

R

K

L

L

T

V

I

I

W

W

M

I

G

N

D

G

N

D

K

K

G

G

Y

Y

D

N

G

G

I

L

R

A

E

E

V

V

A

G

R

K

Q

K

F

F

A

E

D

K

D

D

T

T

G

G

I

I

E

K

V

V

R

T

I

V

V

E

N

H

P

P

D

D

N

K

L

L

T

E

D

E

R

K

F

F

Q

P

Q

Q

A

V

A

A

G

A

S

T

G

G

Q

D

G

G

P

P

D

D

I

I

V

I

I

F

W

W

A

A

H

H

D

D

R

R

I

F

G

G

E

G

W

Y

A

A

Q

Q

S

S

G

G

L

L

L

L

A

A

P

E

V

I

S

T

P

P

S

A

S

A

D

A

F

F

R

Q

E

D

R

K

Y

L

F

Y

D

P

F

F

T

T

W

W

D

D

A

A

T

V

L

R

W

Y

S

N

G

G

E

K

H

L

Y

I

G

A

Y

Y

P

P

I

I

S

A

V

V

E

E

A

A

L

L

G

S

L

L

I

I

Y

Y

N

N

K

K

A

D

L

L

V

L

E

P

T

N

P

P

P

P

E

K

S

T

F

W

A

E

E

E

L

I

A

P

Q

A

L

L

D

D

A

K

E

E

L

L

A

K

E

A

Q

K

G

G

R

K

K

S

A

A

I

L

L

M

F

F

D

N

Y

L

G

Q

E

E

P

P

Y

Y

Y

F

G

T

W

W

T

P

L

L

L

I

A

A

A

A

N

D

G

G

G

G

Y

Y

P

A

F

F

R

K

R

Y

T

A

E

A

E

G

G

K

F

Y

D

D

V

I

D

K

D

D

I

V

G

G

V

V

N

D

N

N

E

A

G

G

A

A

L

K

Q

A

G

G

A

L

E

T

L

F

L

L

V

V

E

D

L

L

I

I

E

K

S

N

G

K

V

H

L

M

P

N

R

A

G

D

T

T

D

D

Y

Y

S

S

I

I

M

A

D

E

T

A

R

A

F

F

N

N

R

K

G

G

E

E

V

T

A

A

A

M

M

T

I

I

S

N

G

G

P

P

W

W

A

A

W

W

S

S

N

N

L

I

E

D

Q

T

S

S

G

A

I

V

D

N

Y

Y

G

G

V

V

A

T

L

V

L

L

P

P

K

T

V

F

G

K

E

G

E

Q

R

P

A

S

K

K

P

P

M

F

F

V

G

G

V

V

M

L

A

S

A

A

M

G

I

I

N

N

T

A

A

A

S

S

P

P

N

N

D

K

F

E

L

L

A

A

V

K

E

E

F

F

L

L

E

E

N

N

Y

Y

L

L

L

L

S

T

E

D

E

E

G

G

M

L

R

E

T

A

F

V

N

N

S

K

D

D

S

K

T

P

L

L

G

G

A

A

V

V

A

A

H

L

I

K

A

S

Y

Y

Q

E

Q

E

E

E

L

L

E

A

S

K

D

D

P

P

N

R

I

I

A

A

A

A

T

T

L

M

E

E

N

N

A

A

E

Q

L

K

G

G

M

E

P

I

M

M

P

P

N

N

I

I

P

P

E

Q

M

M

G

S

A

A

F

F

W

W

A

Y

A

A

M

V

E

R

P

T

A

A

L

V

Q

I

N

N

I

A

G

A

S

S

G

G

R

R

Q

Q

S

T

Sites not aligning to the query:

binding ascorbic acid: 689, 691, 696, 710, 725, 751, 752, 753
binding fe (iii) ion: 699, 701, 751

7cy6A Crystal structure of cmd1 in complex with 5mc-DNA (see paper)
50% identity, 89% coverage: 31:381/395 of query aligns to 5:355/872 of 7cy6A

query
sites
7cy6A

R

K

L

L

T

V

I

I

W

W

M

I

G

N

D

G

N

D

K

K

G

G

Y

Y

D

N

G

G

I

L

R

A

E

E

V

V

A

G

R

K

Q

K

F

F

A

E

D

K

D

D

T

T

G

G

I

I

E

K

V

V

R

T

I

V

V

E

N

H

P

P

D

D

N

K

L

L

T

E

D

E

R

K

F

F

Q

P

Q

Q

A

V

A

A

G

A

S

T

G

G

Q

D

G

G

P

P

D

D

I

I

V

I

I

F

W

W

A

A

H

H

D

D

R

R

I

F

G

G

E

G

W

Y

A

A

Q

Q

S

S

G

G

L

L

L

L

A

A

P

E

V

I

S

T

P

P

S

A

S

A

D

A

F

F

R

Q

E

D

R

K

Y

L

F

Y

D

P

F

F

T

T

W

W

D

D

A

A

T

V

L

R

W

Y

S

N

G

G

E

K

H

L

Y

I

G

A

Y

Y

P

P

I

I

S

A

V

V

E

E

A

A

L

L

G

S

L

L

I

I

Y

Y

N

N

K

K

A

D

L

L

V

L

E

P

T

N

P

P

P

P

E

K

S

T

F

W

A

E

E

E

L

I

A

P

Q

A

L

L

D

D

A

K

E

E

L

L

A

K

E

A

Q

K

G

G

R

K

K

S

A

A

I

L

L

M

F

F

D

N

Y

L

G

Q

E

E

P

P

Y

Y

Y

F

G

T

W

W

T

P

L

L

L

I

A

A

A

A

N

D

G

G

G

G

Y

Y

P

A

F

F

R

K

R

Y

T

A

E

A

E

G

G

K

F

Y

D

D

V

I

D

K

D

D

I

V

G

G

V

V

N

D

N

N

E

A

G

G

A

A

L

K

Q

A

G

G

A

L

E

T

L

F

L

L

V

V

E

D

L

L

I

I

E

K

S

N

G

K

V

H

L

M

P

N

R

A

G

D

T

T

D

D

Y

Y

S

S

I

I

M

A

D

E

T

A

R

A

F

F

N

N

R

K

G

G

E

E

V

T

A

A

A

M

M

T

I

I

S

N

G

G

P

P

W

W

A

A

W

W

S

S

N

N

L

I

E

D

Q

T

S

S

G

A

I

V

D

N

Y

Y

G

G

V

V

A

T

L

V

L

L

P

P

K

T

V

F

G

K

E

G

E

Q

R

P

A

S

K

K

P

P

M

F

F

V

G

G

V

V

M

L

A

S

A

A

M

G

I

I

N

N

T

A

A

A

S

S

P

P

N

N

D

K

F

E

L

L

A

A

V

K

E

E

F

F

L

L

E

E

N

N

Y

Y

L

L

L

L

S

T

E

D

E

E

G

G

M

L

R

E

T

A

F

V

N

N

S

K

D

D

S

K

T

P

L

L

G

G

A

A

V

V

A

A

H

L

I

K

A

S

Y

Y

Q

E

Q

E

E

E

L

L

E

A

S

K

D

D

P

P

N

R

I

I

A

A

A

A

T

T

L

M

E

E

N

N

A

A

E

Q

L

K

G

G

M

E

P

I

M

M

P

P

N

N

I

I

P

P

E

Q

M

M

G

S

A

A

F

F

W

W

A

Y

A

A

M

V

E

R

P

T

A

A

L

V

Q

I

N

N

I

A

G

A

S

S

G

G

R

R

Q

Q

S

T

Sites not aligning to the query:

binding fe (ii) ion: 709, 711, 761
binding : 606, 608, 609, 617, 623, 624, 625, 628, 634, 635, 639, 780, 782, 829, 835, 836

5tj2D Gasdermin-b c-terminal domain containing the polymorphism residues gly299:ser306 fused to maltose binding protein
50% identity, 89% coverage: 31:381/395 of query aligns to 6:356/528 of 5tj2D

query
sites
5tj2D

R

K

L

L

T

V

I

I

W

W

M

I

G

N

D

G

N

D

K
|
K

G

G

Y

Y

D

N

G

G

I

L

R

A

E

E

V

V

A

G

R

K

Q

K

F

F

A

E

D

K

D

D

T

T

G

G

I

I

E

K

V

V

R

T

I

V

V

E

N

H

P

P

D

D

N

K

L

L

T

E

D

E

R

K

F

F

Q

P

Q

Q

A

V

A

A

G

A

S

T

G

G

Q

D

G

G

P

P

D

D

I

I

V

I

I

F

W
|
W

A

A

H

H

D
|
D

R
|
R

I

F

G

G

E

G

W

Y

A

A

Q

Q

S

S

G

G

L

L

L

L

A

A

P

E

V

I

S

T

P

P

S

A

S

A

D

A

F

F

R

Q

E

D

R

K

Y

L

F

Y

D

P

F

F

T

T

W

W

D

D

A

A

T

V

L

R

W

Y

S

N

G

G

E

K

H

L

Y

I

G

A

Y

Y

P

P

I

I

S

A

V

V

E
|
E

A

A

L

L

G

S

L

L

I

I

Y

Y

N

N

K

K

A

D

L

L

V

L

E

P

T

N

P

P

P

P

E

K

S

T

F

W

A

E

E

E

L

I

A

P

Q

A

L

L

D

D

A

K

E

E

L

L

A

K

E

A

Q

K

G

G

R

K

K

S

A

A

I

L

L

M

F

F

D

N

Y

L

G

Q

E
|
E

P
|
P

Y
|
Y

Y
x
F

G

T

W

W

T

P

L

L

L

I

A

A

A

A

N

D

G

G

G

G

Y

Y

P

A

F

F

R

K

R

Y

T

A

E

A

E

G

G

K

F

Y

D

D

V

I

D

K

D

D

I

V

G

G

V

V

N

D

N

N

E

A

G

G

A

A

L

K

Q

A

G

G

A

L

E

T

L

F

L

L

V

V

E

D

L

L

I

I

E

K

S

N

G

K

V

H

L

M

P

N

R

A

G

D

T

T

D

D

Y

Y

S

S

I

I

M

A

D

E

T

A

R

A

F

F

N

N

R

K

G

G

E

E

V

T

A

A

A

M

M

T

I

I

S

N

G

G

P

P

W

W

A

A

W

W

S

S

N

N

L

I

E

D

Q

T

S

S

G

A

I

V

D

N

Y

Y

G

G

V

V

A

T

L

V

L

L

P

P

K

T

V

F

G

K

E

G

E

Q

R

P

A

S

K

K

P

P

M

F

F

V

G

G

V

V

M

L

A

S

A

A

M

G

I

I

N

N

T

A

A

A

S

S

P

P

N

N

D

K

F

E

L

L

A

A

V

K

E

E

F

F

L

L

E

E

N

N

Y

Y

L

L

L

L

S

T

E

D

E

E

G

G

M

L

R

E

T

A

F

V

N

N

S

K

D

D

S

K

T

P

L

L

G

G

A

A

V

V

A

A

H

L

I

K

A

S

Y

Y

Q

E

Q

E

E

E

L

L

E

A

S

K

D

D

P

P

N

R

I

I

A

A

A

A

T

T

L

M

E

E

N

N

A

A

E

Q

L

K

G

G

M

E

P

I

M

M

P

P

N

N

I

I

P

P

E

Q

M

M

G

S

A

A

F

F

W

W

A

Y

A

A

M

V

E

R

P

T

A

A

L

V

Q

I

N

N

I

A

G

A

S

S

G

G

R

R

Q

Q

S

T

binding alpha-D-glucopyranose: K15 (= K40), W62 (= W87), W62 (= W87), D65 (= D90), R66 (= R91), E111 (= E136), E153 (= E178), P154 (= P179), Y155 (= Y180), Y155 (= Y180), F156 (≠ Y181)

5hz7A High-resolution crystal structure of the minor DNA-binding pilin comp from neisseria meningitidis in fusion with mbp (see paper)
50% identity, 89% coverage: 31:381/395 of query aligns to 7:357/490 of 5hz7A

query
sites
5hz7A

R

K

L

L

T

V

I

I

W

W

M

I

G

N

D

G

N
x
D

K
|
K

G

G

Y

Y

D

N

G

G

I

L

R

A

E

E

V

V

A

G

R

K

Q

K

F

F

A

E

D

K

D

D

T

T

G

G

I

I

E

K

V

V

R

T

I

V

V

E

N

H

P

P

D

D

N
x
K

L

L

T
x
E

D
x
E

R

K

F

F

Q

P

Q

Q

A

V

A

A

G

A

S

T

G

G

Q

D

G

G

P

P

D

D

I

I

V

I

I

F

W
|
W

A

A

H

H

D
|
D

R
|
R

I

F

G

G

E

G

W

Y

A

A

Q

Q

S

S

G

G

L

L

L

L

A

A

P

E

V

I

S

T

P

P

S

A

S

A

D

A

F

F

R

Q

E

D

R

K

Y

L

F

Y

D

P

F

F

T

T

W

W

D

D

A

A

T

V

L

R

W

Y

S

N

G

G

E

K

H

L

Y

I

G

A

Y

Y

P

P

I

I

S

A

V

V

E
|
E

A

A

L

L

G

S

L

L

I

I

Y

Y

N

N

K

K

A

D

L

L

V

L

E

P

T

N

P

P

P

P

E

K

S

T

F

W

A

E

E

E

L

I

A

P

Q

A

L

L

D

D

A

K

E

E

L

L

A

K

E

A

Q

K

G

G

R

K

K

S

A

A

I

L

L

M

F

F

D

N

Y

L

G

Q

E
|
E

P
|
P

Y
|
Y

Y

F

G

T

W

W

T

P

L

L

L

I

A

A

A

A

N

D

G

G

G

G

Y

Y

P

A

F

F

R

K

R

Y

T

A

E

A

E

G

G

K

F

Y

D

D

V

I

D

K

D

D

I

V

G

G

V

V

N

D

N

N

E

A

G

G

A

A

L

K

Q

A

G

G

A

L

E

T

L

F

L

L

V

V

E

D

L

L

I

I

E

K

S

N

G

K

V

H

L

M

P

N

R

A

G

D

T

T

D

D

Y

Y

S

S

I

I

M

A

D

E

T

A

R

A

F

F

N

N

R

K

G

G

E

E

V

T

A

A

A

M

M

T

I

I

S

N

G

G

P

P

W
|
W

A

A

W

W

S

S

N

N

L

I

E

D

Q

T

S

S

G

A

I

V

D

N

Y

Y

G

G

V

V

A

T

L

V

L

L

P

P

K

T

V

F

G

K

E

G

E

Q

R

P

A

S

K

K

P

P

M

F

F

V

G

G

V

V

M

L

A

S

A

A

M

G

I

I

N

N

T

A

A

A

S

S

P

P

N

N

D

K

F

E

L

L

A

A

V

K

E

E

F

F

L

L

E

E

N

N

Y

Y

L

L

L

L

S

T

E

D

E

E

G

G

M

L

R

E

T

A

F

V

N

N

S

K

D

D

S

K

T

P

L

L

G

G

A

A

V

V

A

A

H

L

I

K

A

S

Y

Y

Q

E

Q

E

E

E

L

L

E

A

S

K

D

D

P

P

N

R

I

I

A

A

A

A

T

T

L

M

E

E

N

N

A

A

E

Q

L

K

G

G

M

E

P

I

M

M

P

P

N

N

I

I

P

P

E

Q

M

M

G

S

A

A

F

F

W
|
W

A
x
Y

A

A

M

V

E
x
R

P

T

A

A

L

V

Q

I

N

N

I

A

G

A

S

S

G

G

R

R

Q

Q

S

T

binding beta-D-glucopyranose: K43 (≠ N67), E45 (≠ T69), E46 (≠ D70), Y342 (≠ A366)
binding alpha-D-glucopyranose: D15 (≠ N39), K16 (= K40), E45 (≠ T69), W63 (= W87), D66 (= D90), R67 (= R91), E112 (= E136), E154 (= E178), E154 (= E178), P155 (= P179), P155 (= P179), Y156 (= Y180), Y156 (= Y180), W231 (= W255), W341 (= W365), W341 (= W365), Y342 (≠ A366), R345 (≠ E369)

5tj4E Gasdermin-b c-terminal domain containing the polymorphism residues gly299:pro306 fused to maltose binding protein (see paper)
50% identity, 89% coverage: 31:381/395 of query aligns to 6:356/556 of 5tj4E

query
sites
5tj4E

R

K

L

L

T

V

I

I

W

W

M

I

G

N

D

G

N

D

K
|
K

G

G

Y

Y

D

N

G

G

I

L

R

A

E

E

V

V

A

G

R

K

Q

K

F

F

A

E

D

K

D

D

T

T

G

G

I

I

E

K

V

V

R

T

I

V

V

E

N

H

P

P

D

D

N

K

L

L

T

E

D

E

R

K

F

F

Q

P

Q

Q

A

V

A

A

G

A

S

T

G

G

Q

D

G

G

P

P

D

D

I

I

V

I

I

F

W

W

A

A

H

H

D
|
D

R
|
R

I

F

G

G

E

G

W

Y

A

A

Q

Q

S

S

G

G

L

L

L

L

A

A

P

E

V

I

S

T

P

P

S

A

S

A

D

A

F

F

R

Q

E

D

R

K

Y

L

F

Y

D

P

F

F

T

T

W

W

D

D

A

A

T

V

L

R

W

Y

S

N

G

G

E

K

H

L

Y

I

G

A

Y

Y

P

P

I

I

S

A

V

V

E
|
E

A

A

L

L

G

S

L

L

I

I

Y

Y

N

N

K

K

A

D

L

L

V

L

E

P

T

N

P

P

P

P

E

K

S

T

F

W

A

E

E

E

L

I

A

P

Q

A

L

L

D

D

A

K

E

E

L

L

A

K

E

A

Q

K

G

G

R

K

K

S

A

A

I

L

L

M

F

F

D

N

Y

L

G

Q

E
|
E

P
|
P

Y
|
Y

Y

F

G

T

W

W

T

P

L

L

L

I

A

A

A

A

N

D

G

G

G

G

Y

Y

P

A

F

F

R

K

R

Y

T

A

E

A

E

G

G

K

F

Y

D

D

V

I

D

K

D

D

I

V

G

G

V

V

N

D

N

N

E

A

G

G

A

A

L

K

Q

A

G

G

A

L

E

T

L

F

L

L

V

V

E

D

L

L

I

I

E

K

S

N

G

K

V

H

L

M

P

N

R

A

G

D

T

T

D

D

Y

Y

S

S

I

I

M

A

D

E

T

A

R

A

F

F

N

N

R

K

G

G

E

E

V

T

A

A

A

M

M

T

I

I

S

N

G

G

P

P

W
|
W

A

A

W

W

S

S

N

N

L

I

E

D

Q

T

S

S

G

A

I

V

D

N

Y

Y

G

G

V

V

A

T

L

V

L

L

P

P

K

T

V

F

G

K

E

G

E

Q

R

P

A

S

K

K

P

P

M

F

F

V

G

G

V

V

M

L

A

S

A

A

M

G

I

I

N

N

T

A

A

A

S

S

P

P

N

N

D

K

F

E

L

L

A

A

V

K

E

E

F

F

L

L

E

E

N

N

Y

Y

L

L

L

L

S

T

E

D

E

E

G

G

M

L

R

E

T

A

F

V

N

N

S

K

D

D

S

K

T

P

L

L

G

G

A

A

V

V

A

A

H

L

I

K

A

S

Y

Y

Q

E

Q

E

E

E

L

L

E

A

S

K

D

D

P

P

N

R

I

I

A

A

A

A

T

T

L

M

E

E

N

N

A

A

E

Q

L

K

G

G

M

E

P

I

M

M

P

P

N

N

I

I

P

P

E

Q

M

M

G

S

A

A

F

F

W
|
W

A

Y

A

A

M

V

E

R

P

T

A

A

L

V

Q

I

N

N

I

A

G

A

S

S

G

G

R

R

Q

Q

S

T

binding alpha-D-glucopyranose: K15 (= K40), D65 (= D90), R66 (= R91), E111 (= E136), E153 (= E178), P154 (= P179), Y155 (= Y180), W230 (= W255), W340 (= W365)

6sjvA Structure of hpv18 e6 oncoprotein in complex with mutant e6ap lxxll motif
50% identity, 89% coverage: 31:381/395 of query aligns to 6:356/529 of 6sjvA

query
sites
6sjvA

R

K

L

L

T

V

I

I

W

W

M

I

G

N

D

G

N
x
D

K
|
K

G

G

Y

Y

D

N

G

G

I

L

R

A

E

E

V

V

A

G

R

K

Q

K

F

F

A

E

D

K

D

D

T

T

G

G

I

I

E

K

V

V

R

T

I

V

V

E

N

H

P

P

D

D

N

K

L

L

T

E

D

E

R

K

F

F

Q

P

Q

Q

A

V

A

A

G

A

S

T

G

G

Q

D

G

G

P

P

D

D

I

I

V

I

I

F

W
|
W

A

A

H

H

D
|
D

R
|
R

I

F

G

G

E

G

W

Y

A

A

Q

Q

S

S

G

G

L

L

L

L

A

A

P

E

V

I

S

T

P

P

S

A

S

A

D

A

F

F

R

Q

E

D

R

K

Y

L

F

Y

D

P

F

F

T

T

W

W

D

D

A

A

T

V

L

R

W

Y

S

N

G

G

E

K

H

L

Y

I

G

A

Y

Y

P

P

I

I

S

A

V

V

E
|
E

A

A

L

L

G

S

L

L

I

I

Y

Y

N

N

K

K

A

D

L

L

V

L

E

P

T

N

P

P

P

P

E

K

S

T

F

W

A

E

E

E

L

I

A

P

Q

A

L

L

D

D

A

K

E

E

L

L

A

K

E

A

Q

K

G

G

R

K

K

S

A

A

I

L

L

M

F

F

D

N

Y

L

G

Q

E
|
E

P
|
P

Y
|
Y

Y

F

G

T

W

W

T

P

L

L

L

I

A

A

A

A

N

D

G

G

G

G

Y

Y

P

A

F

F

R

K

R

Y

T

E

E

N

E

G

G

K

F

Y

D

D

V

I

D

K

D

D

I

V

G

G

V

V

N

D

N

N

E

A

G

G

A

A

L

K

Q

A

G

G

A

L

E

T

L

F

L

L

V

V

E

D

L

L

I

I

E

K

S

N

G

K

V

H

L

M

P

N

R

A

G

D

T

T

D

D

Y

Y

S

S

I

I

M

A

D

E

T

A

R

A

F

F

N

N

R

K

G

G

E

E

V

T

A

A

A

M

M

T

I

I

S

N

G

G

P

P

W
|
W

A

A

W

W

S

S

N

N

L

I

E

D

Q

T

S

S

G

A

I

V

D

N

Y

Y

G

G

V

V

A

T

L

V

L

L

P

P

K

T

V

F

G

K

E

G

E

Q

R

P

A

S

K

K

P

P

M

F

F

V

G

G

V

V

M

L

A

S

A

A

M

G

I

I

N

N

T

A

A

A

S

S

P

P

N

N

D

K

F

E

L

L

A

A

V

K

E

E

F

F

L

L

E

E

N

N

Y

Y

L

L

L

L

S

T

E

D

E

E

G

G

M

L

R

E

T

A

F

V

N

N

S

K

D

D

S

K

T

P

L

L

G

G

A

A

V

V

A

A

H

L

I

K

A

S

Y

Y

Q

E

Q

E

E

E

L

L

E

A

S

K

D

D

P

P

N

R

I

I

A

A

A

A

T

T

L

M

E

E

N

N

A

A

E

Q

L

K

G

G

M

E

P

I

M

M

P

P

N

N

I

I

P

P

E

Q

M

M

G

S

A

A

F

F

W
|
W

A

Y

A

A

M

V

E

R

P

T

A

A

L

V

Q

I

N

N

I

A

G

A

S

S

G

G

R

R

Q

Q

S

T

binding alpha-D-glucopyranose: D14 (≠ N39), K15 (= K40), W62 (= W87), D65 (= D90), R66 (= R91), E111 (= E136), E153 (= E178), P154 (= P179), Y155 (= Y180), W230 (= W255), W340 (= W365)

Sites not aligning to the query:

binding zinc ion: 402, 405, 435, 438, 475, 478, 508, 511

6d67A Crystal structure of the human dual specificity phosphatase 1 catalytic domain (c258s) as a maltose binding protein fusion (maltose bound form) in complex with the designed ar protein mbp3_16 (see paper)
50% identity, 89% coverage: 31:381/395 of query aligns to 6:356/514 of 6d67A

query
sites
6d67A

R

K

L

L

T

V

I

I

W

W

M

I

G

N

D

G

N
x
D

K
|
K

G

G

Y

Y

D

N

G

G

I

L

R

A

E

E

V

V

A

G

R

K

Q

K

F

F

A

E

D

K

D

D

T

T

G

G

I

I

E

K

V

V

R

T

I

V

V

E

N

H

P

P

D

D

N

K

L

L

T

E

D

E

R

K

F

F

Q

P

Q

Q

A

V

A

A

G

A

S

T

G

G

Q

D

G

G

P

P

D

D

I

I

V

I

I

F

W
|
W

A

A

H

H

D
|
D

R
|
R

I

F

G

G

E

G

W

Y

A

A

Q

Q

S

S

G

G

L

L

L

L

A

A

P

E

V

I

S

T

P

P

S

A

S

A

D

A

F

F

R

Q

E

D

R

K

Y

L

F

Y

D

P

F

F

T

T

W

W

D

D

A

A

T

V

L

R

W

Y

S

N

G

G

E

K

H

L

Y

I

G

A

Y

Y

P

P

I

I

S

A

V

V

E
|
E

A

A

L

L

G

S

L

L

I

I

Y

Y

N

N

K

K

A

D

L

L

V

L

E

P

T

N

P

P

P

P

E

K

S

T

F

W

A

E

E

E

L

I

A

P

Q

A

L

L

D

D

A

K

E

E

L

L

A

K

E

A

Q

K

G

G

R

K

K

S

A

A

I

L

L

M

F

F

D

N

Y

L

G

Q

E
|
E

P
|
P

Y
|
Y

Y

F

G

T

W

W

T

P

L

L

L

I

A

A

A

A

N

D

G

G

G

G

Y

Y

P

A

F

F

R

K

R

Y

T

A

E

A

E

G

G

K

F

Y

D

D

V

I

D

K

D

D

I

V

G

G

V

V

N

D

N

N

E

A

G

G

A

A

L

K

Q

A

G

G

A

L

E

T

L

F

L

L

V

V

E

D

L

L

I

I

E

K

S

N

G

K

V

H

L

M

P

N

R

A

G

D

T

T

D

D

Y

Y

S

S

I

I

M

A

D

E

T

A

R

A

F

F

N

N

R

K

G

G

E

E

V

T

A

A

A

M

M

T

I

I

S

N

G

G

P

P

W
|
W

A

A

W

W

S

S

N

N

L

I

E

D

Q

T

S

S

G

A

I

V

D

N

Y

Y

G

G

V

V

A

T

L

V

L

L

P

P

K

T

V

F

G

K

E

G

E

Q

R

P

A

S

K

K

P

P

M

F

F

V

G

G

V

V

M

L

A

S

A

A

M

G

I

I

N

N

T

A

A

A

S

S

P

P

N

N

D

K

F

E

L

L

A

A

V

K

E

E

F

F

L

L

E

E

N

N

Y

Y

L

L

L

L

S

T

E

D

E

E

G

G

M

L

R

E

T

A

F

V

N

N

S

K

D

D

S

K

T

P

L

L

G

G

A

A

V

V

A

A

H

L

I

K

A

S

Y

Y

Q

E

Q

E

E

E

L

L

E

A

S

K

D

D

P

P

N

R

I

I

A

A

A

A

T

T

L

M

E

E

N

N

A

A

E

Q

L

K

G

G

M

E

P

I

M

M

P

P

N

N

I

I

P

P

E

Q

M

M

G

S

A

A

F

F

W
|
W

A

Y

A

A

M

V

E

R

P

T

A

A

L

V

Q

I

N

N

I

A

G

A

S

S

G

G

R

R

Q

Q

S

T

binding alpha-D-glucopyranose: D14 (≠ N39), K15 (= K40), W62 (= W87), D65 (= D90), R66 (= R91), E111 (= E136), E153 (= E178), P154 (= P179), Y155 (= Y180), Y155 (= Y180), W230 (= W255), W340 (= W365)

1hsjA Sarr mbp fusion structure (see paper)
50% identity, 90% coverage: 31:384/395 of query aligns to 6:359/487 of 1hsjA

query
sites
1hsjA

R

K

L

L

T

V

I

I

W

W

M

I

G

N

D

G

N
x
D

K
|
K

G

G

Y

Y

D

N

G

G

I

L

R

A

E

E

V

V

A

G

R

K

Q

K

F

F

A

E

D

K

D

D

T

T

G

G

I

I

E

K

V

V

R

T

I

V

V

E

N

H

P

P

D

D

N

K

L

L

T

E

D

E

R

K

F

F

Q

P

Q

Q

A

V

A

A

G

A

S

T

G

G

Q

D

G

G

P

P

D

D

I

I

V

I

I

F

W
|
W

A

A

H

H

D
|
D

R
|
R

I

F

G

G

E

G

W

Y

A

A

Q

Q

S

S

G

G

L

L

L

L

A

A

P

E

V

I

S

T

P

P

S

D

S

K

D

A

F

F

R

Q

E

D

R

K

Y

L

F

Y

D

P

F

F

T

T

W

W

D

D

A

A

T

V

L

R

W

Y

S

N

G

G

E

K

H

L

Y

I

G

A

Y

Y

P

P

I

I

S

A

V

V

E
|
E

A

A

L

L

G

S

L

L

I

I

Y

Y

N

N

K

K

A

D

L

L

V

L

E

P

T

N

P

P

P

P

E

K

S

T

F

W

A

E

E

E

L

I

A

P

Q

A

L

L

D

D

A

K

E

E

L

L

A

K

E

A

Q

K

G

G

R

K

K

S

A

A

I

L

L

M

F

F

D

N

Y

L

G

Q

E
|
E

P
|
P

Y
|
Y

Y

F

G

T

W

W

T

P

L

L

L

I

A

A

A

A

N

D

G

G

G

G

Y

Y

P

A

F

F

R

K

R

Y

T

E

E

N

E

G

G

K

F

Y

D

D

V

I

D

K

D

D

I

V

G

G

V

V

N

D

N

N

E

A

G

G

A

A

L

K

Q

A

G

G

A

L

E

T

L

F

L

L

V

V

E

D

L

L

I

I

E

K

S

N

G

K

V

H

L

M

P

N

R

A

G

D

T

T

D

D

Y

Y

S

S

I

I

M

A

D

E

T

A

R

A

F

F

N

N

R

K

G

G

E

E

V

T

A

A

A

M

M

T

I

I

S

N

G

G

P

P

W
|
W

A

A

W

W

S

S

N

N

L

I

E

D

Q

T

S

S

G

K

I

V

D

N

Y

Y

G

G

V

V

A

T

L

V

L

L

P

P

K

T

V

F

G

K

E

G

E

Q

R

P

A

S

K

K

P

P

M

F

F

V

G

G

V

V

M

L

A

S

A

A

M

G

I

I

N

N

T

A

A

A

S

S

P

P

N

N

D

K

F

E

L

L

A

A

V

K

E

E

F

F

L

L

E

E

N

N

Y

Y

L

L

L

L

S

T

E

D

E

E

G

G

M

L

R

E

T

A

F

V

N

N

S

K

D

D

S

K

T

P

L

L

G

G

A

A

V

V

A

A

H

L

I

K

A

S

Y

Y

Q

E

Q

E

E

E

L

L

E

A

S

K

D

D

P

P

N

R

I

I

A

A

A

A

T

T

L

M

E

E

N

N

A

A

E

Q

L

K

G

G

M

E

P

I

M

M

P

P

N

N

I

I

P

P

E

Q

M

M

G

S

A

A

F

F

W
|
W

A

Y

A

A

M

V

E
x
R

P

T

A

A

L

V

Q

I

N

N

I

A

G

A

S

S

G

G

R

R

Q

Q

S

T

P

V

R

D

E

E

binding alpha-D-glucopyranose: D14 (≠ N39), K15 (= K40), W62 (= W87), D65 (= D90), R66 (= R91), E111 (= E136), E153 (= E178), P154 (= P179), Y155 (= Y180), W230 (= W255), W340 (= W365), R344 (≠ E369)

8c5lB Nr2f6 ligand binding domain in complex with nsd1 peptide
50% identity, 89% coverage: 31:381/395 of query aligns to 7:357/557 of 8c5lB

query
sites
8c5lB

R

K

L

L

T

V

I

I

W

W

M

I

G

N

D

G

N
x
D

K
|
K

G

G

Y

Y

D

N

G

G

I

L

R

A

E

E

V

V

A

G

R

K

Q

K

F

F

A

E

D

K

D

D

T

T

G

G

I

I

E

K

V

V

R

T

I

V

V

E

N

H

P

P

D

D

N

K

L

L

T

E

D

E

R

K

F

F

Q

P

Q

Q

A

V

A

A

G

A

S

T

G

G

Q

D

G

G

P

P

D

D

I

I

V

I

I

F

W
|
W

A

A

H

H

D
|
D

R
|
R

I

F

G

G

E

G

W

Y

A

A

Q

Q

S

S

G

G

L

L

L

L

A

A

P

E

V

I

S

T

P

P

S

A

S

A

D

A

F

F

R

Q

E

D

R

K

Y

L

F

Y

D

P

F

F

T

T

W

W

D

D

A

A

T

V

L

R

W

Y

S

N

G

G

E

K

H

L

Y

I

G

A

Y

Y

P

P

I

I

S

A

V

V

E
|
E

A

A

L

L

G

S

L

L

I

I

Y

Y

N

N

K

K

A

D

L

L

V

L

E

P

T

N

P

P

P

P

E

K

S

T

F

W

A

E

E

E

L

I

A

P

Q

A

L

L

D

D

A

K

E

E

L

L

A

K

E

A

Q

K

G

G

R

K

K

S

A

A

I

L

L

M

F

F

D

N

Y

L

G

Q

E
|
E

P
|
P

Y
|
Y

Y

F

G

T

W

W

T

P

L

L

L

I

A

A

A

A

N

D

G

G

G

G

Y

Y

P

A

F

F

R

K

R

Y

T

A

E

A

E

G

G

K

F

Y

D

D

V

I

D

K

D

D

I

V

G

G

V

V

N

D

N

N

E

A

G

G

A

A

L

K

Q

A

G

G

A

L

E

T

L

F

L

L

V

V

E

D

L

L

I

I

E

K

S

N

G

K

V

H

L

M

P

N

R

A

G

D

T

T

D

D

Y

Y

S

S

I

I

M

A

D

E

T

A

R

A

F

F

N

N

R

K

G

G

E

E

V

T

A

A

A

M

M

T

I

I

S

N

G

G

P

P

W
|
W

A

A

W

W

S

S

N

N

L

I

E

D

Q

T

S

S

G

A

I

V

D

N

Y

Y

G

G

V

V

A

T

L

V

L

L

P

P

K

T

V

F

G

K

E

G

E

Q

R

P

A

S

K

K

P

P

M

F

F

V

G

G

V

V

M

L

A

S

A

A

M

G

I

I

N

N

T

A

A

A

S

S

P

P

N

N

D

K

F

E

L

L

A

A

V

K

E

E

F

F

L

L

E

E

N

N

Y

Y

L

L

L

L

S

T

E

D

E

E

G

G

M

L

R

E

T

A

F

V

N

N

S

K

D

D

S

K

T

P

L

L

G

G

A

A

V

V

A

A

H

L

I

K

A

S

Y

Y

Q

E

Q

E

E

E

L

L

E

A

S

K

D

D

P

P

N

R

I

I

A

A

A

A

T

T

L

M

E

E

N

N

A

A

E

Q

L

K

G

G

M

E

P

I

M

M

P

P

N

N

I

I

P

P

E

Q

M

M

G

S

A

A

F

F

W
|
W

A

Y

A

A

M

V

E

R

P

T

A

A

L

V

Q

I

N

N

I

A

G

A

S

S

G

G

R

R

Q

Q

S

T

binding alpha-D-glucopyranose: D15 (≠ N39), K16 (= K40), W63 (= W87), D66 (= D90), R67 (= R91), E112 (= E136), E154 (= E178), P155 (= P179), Y156 (= Y180), W231 (= W255), W341 (= W365)

Sites not aligning to the query:

binding : 375, 379, 382, 383, 386, 540, 541, 544, 553

6i4yA X-ray structure of the human mitochondrial prelid3b-triap1 complex (see paper)
50% identity, 89% coverage: 31:381/395 of query aligns to 6:356/429 of 6i4yA

query
sites
6i4yA

R

K

L

L

T

V

I

I

W

W

M

I

G

N

D

G

N
x
D

K
|
K

G

G

Y

Y

D

N

G

G

I

L

R

A

E

E

V

V

A

G

R

K

Q

K

F

F

A

E

D

K

D

D

T

T

G

G

I

I

E

K

V

V

R

T

I

V

V

E

N

H

P

P

D

D

N

K

L

L

T

E

D

E

R

K

F

F

Q

P

Q

Q

A

V

A

A

G

A

S

T

G

G

Q

D

G

G

P

P

D

D

I

I

V

I

I

F

W
|
W

A

A

H

H

D
|
D

R
|
R

I

F

G

G

E

G

W

Y

A

A

Q

Q

S

S

G

G

L

L

L

L

A

A

P

E

V

I

S

T

P

P

S

A

S

A

D

A

F

F

R

Q

E

D

R

K

Y

L

F

Y

D

P

F

F

T

T

W

W

D

D

A

A

T

V

L

R

W

Y

S

N

G

G

E

K

H

L

Y

I

G

A

Y

Y

P

P

I

I

S

A

V

V

E
|
E

A

A

L

L

G

S

L

L

I

I

Y

Y

N

N

K

K

A

D

L

L

V

L

E

P

T

N

P

P

P

P

E

K

S

T

F

W

A

E

E

E

L

I

A

P

Q

A

L

L

D

D

A

K

E

E

L

L

A

K

E

A

Q

K

G

G

R

K

K

S

A

A

I

L

L

M

F

F

D

N

Y

L

G

Q

E
|
E

P
|
P

Y
|
Y

Y

F

G

T

W

W

T

P

L

L

L

I

A

A

A

A

N

D

G

G

G

G

Y

Y

P

A

F

F

R

K

R

Y

T

A

E

A

E

G

G

K

F

Y

D

D

V

I

D

K

D

D

I

V

G

G

V

V

N

D

N

N

E

A

G

G

A

A

L

K

Q

A

G

G

A

L

E

T

L

F

L

L

V

V

E

D

L

L

I

I

E

K

S

N

G

K

V

H

L

M

P

N

R

A

G

D

T

T

D

D

Y

Y

S

S

I

I

M

A

D

E

T

A

R

A

F

F

N

N

R

K

G

G

E

E

V

T

A

A

A

M

M

T

I

I

S

N

G

G

P

P

W
|
W

A

A

W

W

S

S

N

N

L

I

E

D

Q

T

S

S

G

A

I

V

D

N

Y

Y

G

G

V

V

A

T

L

V

L

L

P

P

K

T

V

F

G

K

E

G

E

Q

R

P

A

S

K

K

P

P

M

F

F

V

G

G

V

V

M

L

A

S

A

A

M

G

I

I

N

N

T

A

A

A

S

S

P

P

N

N

D

K

F

E

L

L

A

A

V

K

E

E

F

F

L

L

E

E

N

N

Y

Y

L

L

L

L

S

T

E

D

E

E

G

G

M

L

R

E

T

A

F

V

N

N

S

K

D

D

S

K

T

P

L

L

G

G

A

A

V

V

A

A

H

L

I

K

A

S

Y

Y

Q

E

Q

E

E

E

L

L

E

A

S

K

D

D

P

P

N

R

I

I

A

A

A

A

T

T

L

M

E

E

N

N

A

A

E

Q

L

K

G

G

M

E

P

I

M

M

P

P

N

N

I

I

P

P

E

Q

M

M

G

S

A

A

F

F

W
|
W

A

Y

A

A

M

V

E

R

P

T

A

A

L

V

Q

I

N

N

I

A

G

A

S

S

G

G

R

R

Q

Q

S

T

binding alpha-D-glucopyranose: D14 (≠ N39), K15 (= K40), W62 (= W87), D65 (= D90), R66 (= R91), E111 (= E136), E153 (= E178), E153 (= E178), P154 (= P179), Y155 (= Y180), Y155 (= Y180), W230 (= W255), W340 (= W365)

Query Sequence

>WP_086508157.1 NCBI__GCF_002151265.1:WP_086508157.1
MHRTRHGVLLPVLVALGSGLAFSTYAFDGDRLTIWMGDNKGYDGIREVARQFADDTGIEV
RIVNPDNLTDRFQQAAGSGQGPDIVIWAHDRIGEWAQSGLLAPVSPSSDFRERYFDFTWD
ATLWSGEHYGYPISVEALGLIYNKALVETPPESFAELAQLDAELAEQGRKAILFDYGEPY
YGWTLLAANGGYPFRRTEEGFDVDDIGVNNEGALQGAELLVELIESGVLPRGTDYSIMDT
RFNRGEVAAMISGPWAWSNLEQSGIDYGVALLPKVGEERAKPMFGVMAAMINTASPNDFL
AVEFLENYLLSEEGMRTFNSDSTLGAVAHIAYQQELESDPNIAATLENAELGMPMPNIPE
MGAFWAAMEPALQNIGSGRQSPREALDAAARRMRQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory