SitesBLAST
Comparing WP_086508321.1 NCBI__GCF_002151265.1:WP_086508321.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
2vpyF Polysulfide reductase with bound quinone inhibitor, pentachlorophenol (pcp) (see paper)
48% identity, 73% coverage: 46:234/259 of query aligns to 3:188/193 of 2vpyF
- binding pentachlorophenol: I91 (≠ V136), C93 (= C138)
- binding iron/sulfur cluster: C13 (= C56), G15 (= G58), C16 (= C59), C19 (= C62), C23 (= C66), N27 (= N70), L36 (≠ T79), I38 (≠ V81), P55 (= P100), Q57 (≠ L102), C58 (= C103), L59 (≠ N104), C61 (= C106), P65 (= P110), C66 (= C111), C70 (= C115), P71 (= P116), V83 (= V128), C90 (= C135), I91 (≠ V136), A92 (≠ G137), C93 (= C138), G94 (≠ A139), C96 (= C141), C100 (= C145), P101 (= P146), Y102 (= Y147), R105 (= R150), V113 (≠ T158), C116 (= C162), F118 (= F164), C119 (= C165), P129 (= P175), A130 (= A176), C131 (= C177), C135 (= C181), C139 (≠ A185), R140 (= R186)
Sites not aligning to the query:
2vpwF Polysulfide reductase with bound menaquinone (see paper)
48% identity, 73% coverage: 46:234/259 of query aligns to 3:188/193 of 2vpwF
- binding iron/sulfur cluster: C13 (= C56), G15 (= G58), C16 (= C59), C19 (= C62), C23 (= C66), N27 (= N70), L36 (≠ T79), I38 (≠ V81), P55 (= P100), Q57 (≠ L102), C58 (= C103), L59 (≠ N104), H60 (= H105), C61 (= C106), P65 (= P110), C66 (= C111), C70 (= C115), P71 (= P116), V83 (= V128), C90 (= C135), I91 (≠ V136), A92 (≠ G137), C93 (= C138), G94 (≠ A139), C96 (= C141), C100 (= C145), P101 (= P146), Y102 (= Y147), R105 (= R150), V113 (≠ T158), C116 (= C162), F118 (= F164), C119 (= C165), P129 (= P175), A130 (= A176), C131 (= C177), C135 (= C181), C139 (≠ A185), R140 (= R186)
Sites not aligning to the query:
6cz7B The arsenate respiratory reductase (arr) complex from shewanella sp. Ana-3 (see paper)
35% identity, 69% coverage: 46:225/259 of query aligns to 2:225/234 of 6cz7B
- binding iron/sulfur cluster: C12 (= C56), V13 (≠ I57), G14 (= G58), C15 (= C59), G16 (≠ Q60), C18 (= C62), C22 (= C66), N26 (= N70), Y52 (≠ M98), P54 (= P100), C57 (= C103), N58 (= N104), H59 (= H105), C60 (= C106), A63 (≠ P109), P64 (= P110), C65 (= C111), C69 (= C115), P70 (= P116), T82 (≠ V128), C89 (= C135), I90 (≠ V136), G91 (= G137), C92 (= C138), K93 (≠ A139), C95 (= C141), C99 (= C145), Y101 (= Y147), V103 (≠ A149), I104 (≠ R150), T161 (≠ A159), K163 (= K161), C164 (= C162), F166 (= F164), C167 (= C165), C179 (= C177), C183 (= C181), P184 (≠ V182), R188 (= R186)
Q7WTT9 Arsenate respiratory reductase iron-sulfur subunit ArrB; Arsenate respiratory reductase small subunit; ARR small subunit from Shewanella sp. (strain ANA-3) (see paper)
35% identity, 69% coverage: 46:225/259 of query aligns to 2:225/234 of Q7WTT9
- C12 (= C56) binding
- C15 (= C59) binding
- C18 (= C62) binding
- C22 (= C66) binding
- C57 (= C103) binding
- C60 (= C106) binding
- C65 (= C111) binding
- C69 (= C115) binding
- C89 (= C135) binding
- C92 (= C138) binding
- C95 (= C141) binding
- C99 (= C145) binding
- C164 (= C162) binding
- C167 (= C165) binding
- C179 (= C177) binding
- C183 (= C181) binding
5ch7B Crystal structure of the perchlorate reductase pcrab - phe164 gate switch intermediate - from azospira suillum ps (see paper)
44% identity, 54% coverage: 87:225/259 of query aligns to 114:255/329 of 5ch7B
- binding fe3-s4 cluster: C145 (= C115), I150 (≠ T120), C166 (= C135), K167 (≠ V136), G168 (= G137), A169 (≠ C138), Q170 (≠ A139), A171 (≠ Y140), C172 (= C141), A190 (= A159)
- binding iron/sulfur cluster: P130 (= P100), C133 (= C103), N134 (= N104), C136 (= C106), P139 (= P109), C141 (= C111), C176 (= C145), C193 (= C162), G195 (≠ F164), C196 (= C165), C208 (= C177), C212 (= C181), V213 (= V182), G214 (= G183)
Sites not aligning to the query:
2ivfB Ethylbenzene dehydrogenase from aromatoleum aromaticum (see paper)
42% identity, 50% coverage: 97:225/259 of query aligns to 130:258/337 of 2ivfB
- binding fe3-s4 cluster: C148 (= C115), T150 (≠ V117), I153 (≠ T120), C169 (= C135), K170 (≠ V136), G171 (= G137), H172 (≠ C138), R173 (≠ A139), H174 (≠ Y140), C175 (= C141), S193 (≠ A159)
- binding protoporphyrin ix containing fe: T150 (≠ V117), K170 (≠ V136), H172 (≠ C138)
- binding iron/sulfur cluster: M135 (≠ L102), C136 (= C103), N137 (= N104), H138 (= H105), C139 (= C106), P142 (= P109), C144 (= C111), V162 (= V128), C179 (= C145), A183 (= A149), I184 (≠ R150), K195 (= K161), C196 (= C162), I197 (≠ T163), L198 (≠ F164), C199 (= C165), N209 (≠ P175), C211 (= C177), C215 (= C181), V219 (≠ A185), R220 (= R186)
Sites not aligning to the query:
- binding iron/sulfur cluster: 13, 14, 15, 16, 17, 19, 23, 27, 38, 39, 41
1kqfB Formate dehydrogenase n from e. Coli (see paper)
31% identity, 76% coverage: 29:225/259 of query aligns to 11:226/289 of 1kqfB
- binding protoporphyrin ix containing fe: Y137 (= Y140)
- binding iron/sulfur cluster: K31 (≠ M49), C38 (= C56), I39 (= I57), G40 (= G58), C41 (= C59), K42 (≠ Q60), C44 (= C62), C48 (= C66), N52 (= N70), T77 (= T80), M79 (≠ S82), C99 (= C103), M100 (≠ N104), H101 (= H105), C102 (= C106), P105 (= P109), C107 (= C111), C111 (= C115), P112 (= P116), I117 (≠ T120), V125 (= V128), C132 (= C135), I133 (≠ V136), G134 (= G137), C135 (= C138), G136 (≠ A139), Y137 (= Y140), C138 (= C141), C142 (= C145), I146 (≠ A149), P147 (≠ R150), V156 (≠ A159), K158 (= K161), C159 (= C162), L161 (≠ F164), C162 (= C165), P172 (= P175), C174 (= C177), C178 (= C181), P179 (≠ V182), I183 (≠ R186)
Sites not aligning to the query:
P0AAJ3 Formate dehydrogenase, nitrate-inducible, iron-sulfur subunit; Anaerobic formate dehydrogenase iron-sulfur subunit; Formate dehydrogenase-N subunit beta; FDH-N subunit beta from Escherichia coli (strain K12) (see paper)
31% identity, 76% coverage: 29:225/259 of query aligns to 12:227/294 of P0AAJ3
- C39 (= C56) binding
- C42 (= C59) binding
- C45 (= C62) binding
- C49 (= C66) binding
- C100 (= C103) binding
- C103 (= C106) binding
- C108 (= C111) binding
- C112 (= C115) binding
- C133 (= C135) binding
- C136 (= C138) binding
- C139 (= C141) binding
- C143 (= C145) binding
- C160 (= C162) binding
- C163 (= C165) binding
- C175 (= C177) binding
- C179 (= C181) binding
P18776 Anaerobic dimethyl sulfoxide reductase chain B; DMSO reductase iron-sulfur subunit from Escherichia coli (strain K12) (see 2 papers)
34% identity, 68% coverage: 44:220/259 of query aligns to 2:191/205 of P18776
- C102 (= C138) mutation C->F,S,W,Y: Loss of electron transfer from menaquinol to DMSO.
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
3egwB The crystal structure of the narghi mutant narh - c16a
37% identity, 58% coverage: 96:246/259 of query aligns to 175:323/509 of 3egwB
- binding fe3-s4 cluster: P181 (= P100), C196 (= C115), S198 (≠ V117), I201 (≠ T120), C217 (= C135), R218 (≠ V136), G219 (= G137), W220 (≠ C138), R221 (≠ A139), C223 (= C141), S241 (≠ A159), C263 (= C181), G265 (= G183), R268 (= R186)
- binding protoporphyrin ix containing fe: W220 (≠ C138), R221 (≠ A139)
- binding iron/sulfur cluster: C184 (= C103), E185 (≠ N104), H186 (= H105), C187 (= C106), P190 (= P109), C192 (= C111), C227 (= C145), I232 (≠ R150), K243 (= K161), C244 (= C162), F246 (= F164), C247 (= C165), T257 (≠ P175), C259 (= C177)
Sites not aligning to the query:
P11349 Respiratory nitrate reductase 1 beta chain; Nitrate reductase A subunit beta; Quinol-nitrate oxidoreductase subunit beta; EC 1.7.5.1 from Escherichia coli (strain K12) (see 2 papers)
37% identity, 58% coverage: 96:246/259 of query aligns to 175:323/512 of P11349
- C184 (= C103) binding
- C187 (= C106) binding
- C192 (= C111) binding
- C196 (= C115) binding
- C217 (= C135) binding
- C223 (= C141) binding
- C227 (= C145) binding
- C244 (= C162) binding
- C247 (= C165) binding
- C259 (= C177) binding
- C263 (= C181) binding
Sites not aligning to the query:
- 16 binding
- 19 binding
- 22 binding
- 26 binding
8k9fB Cryo-em structure of the photosynthetic alternative complex iii from chloroflexus aurantiacus at 2.9 angstrom (see paper)
30% identity, 69% coverage: 47:225/259 of query aligns to 710:939/951 of 8k9fB
- binding fe3-s4 cluster: C787 (= C115), P788 (= P116), M802 (≠ V130), C807 (= C135), G809 (= G137), T810 (≠ C138), K811 (≠ A139), Y812 (= Y140), C813 (= C141)
- binding heme c: N805 (≠ D133), R806 (= R134), R867 (vs. gap), K871 (vs. gap)
- binding iron/sulfur cluster: C719 (= C56), G721 (= G58), C722 (= C59), N723 (≠ Q60), C725 (= C62), C729 (= C66), N733 (= N70), W751 (≠ Q83), I752 (≠ Y84), P772 (= P100), C775 (= C103), M776 (≠ N104), Q777 (≠ H105), C778 (= C106), C783 (= C111), N800 (≠ V128), C817 (= C145), Y819 (= Y147), V821 (≠ A149), C856 (= C162), Y858 (≠ F164), C859 (= C165), T891 (≠ P175), C893 (= C177), C897 (= C181), P898 (≠ V182), T899 (≠ G183)
Sites not aligning to the query:
8k9eB Cryo-em structure of the photosynthetic alternative complex iii from chloroflexus aurantiacus at 3.3 angstrom (see paper)
30% identity, 69% coverage: 47:225/259 of query aligns to 710:939/951 of 8k9eB
- binding fe3-s4 cluster: C787 (= C115), M802 (≠ V130), C807 (= C135), V808 (= V136), G809 (= G137), T810 (≠ C138), K811 (≠ A139), Y812 (= Y140), C813 (= C141), M853 (≠ A159)
- binding heme c: N805 (≠ D133), R867 (vs. gap), K871 (vs. gap)
- binding iron/sulfur cluster: C719 (= C56), I720 (= I57), G721 (= G58), C722 (= C59), N723 (≠ Q60), C725 (= C62), C729 (= C66), N733 (= N70), I754 (≠ V86), C775 (= C103), M776 (≠ N104), C778 (= C106), P782 (= P110), C783 (= C111), N800 (≠ V128), C817 (= C145), Y819 (= Y147), V821 (≠ A149), R822 (= R150), C856 (= C162), Y858 (≠ F164), C859 (= C165), T891 (≠ P175), C893 (= C177), C897 (= C181), T899 (≠ G183)
Sites not aligning to the query:
6btmB Structure of alternative complex iii from flavobacterium johnsoniae (wild type) (see paper)
25% identity, 69% coverage: 47:224/259 of query aligns to 684:942/948 of 6btmB
- binding fe3-s4 cluster: C789 (= C115), C809 (= C135), V810 (= V136), T812 (≠ C138), C815 (= C141), M862 (vs. gap)
- binding iron/sulfur cluster: C777 (= C103), Q778 (≠ N104), C780 (= C106), C785 (= C111), N802 (≠ V128), C819 (= C145), R824 (= R150)
Sites not aligning to the query:
6f0kB Alternative complex iii (see paper)
29% identity, 75% coverage: 33:226/259 of query aligns to 709:949/961 of 6f0kB
- binding fe3-s4 cluster: C803 (= C115), V805 (= V117), M818 (≠ V130), C823 (= C135), I824 (≠ V136), G825 (= G137), C829 (= C141), M869 (vs. gap)
- binding heme c: Y719 (≠ P43), N821 (≠ D133), R822 (= R134), R884 (vs. gap), N887 (vs. gap)
- binding iron/sulfur cluster: C733 (= C56), T734 (≠ I57), G735 (= G58), C736 (= C59), N737 (≠ Q60), A738 (= A61), C739 (= C62), C743 (= C66), W765 (vs. gap), I768 (≠ V81), C791 (= C103), M792 (≠ N104), H793 (= H105), C794 (= C106), P798 (= P110), C799 (= C111), N816 (≠ V128), C833 (= C145), C872 (= C162), Y874 (≠ F164), C875 (= C165), A901 (= A176), C902 (= C177), C906 (= C181)
6lodB Cryo-em structure of the air-oxidized photosynthetic alternative complex iii from roseiflexus castenholzii (see paper)
29% identity, 69% coverage: 46:225/259 of query aligns to 682:917/929 of 6lodB
- binding fe3-s4 cluster: V758 (= V114), C759 (= C115), P760 (= P116), C779 (= C135), V780 (= V136), G781 (= G137), T782 (≠ C138), C785 (= C141), M838 (vs. gap)
- binding heme c: N777 (≠ D133), R778 (= R134), R852 (vs. gap), I853 (vs. gap), R856 (vs. gap)
- binding iron/sulfur cluster: C692 (= C56), N693 (≠ I57), S694 (≠ G58), C695 (= C59), N696 (≠ Q60), A697 (= A61), C698 (= C62), C702 (= C66), N706 (= N70), W724 (≠ R78), I727 (≠ V81), L746 (= L102), C747 (= C103), Q748 (≠ N104), Q749 (≠ H105), C750 (= C106), P754 (= P110), C755 (= C111), N772 (≠ V128), C789 (= C145), Y791 (= Y147), V793 (≠ A149), R794 (= R150), K840 (= K161), C841 (= C162), F843 (= F164), C844 (= C165), T869 (≠ P175), C871 (= C177), C875 (= C181), I880 (≠ R186)
7b04A of Nitrite oxidoreductase (Nxr) from the anammox bacterium Kuenenia stuttgartiensis.
35% identity, 50% coverage: 97:225/259 of query aligns to 168:305/409 of 7b04A
- binding fe3-s4 cluster: C186 (= C115), I191 (≠ T120), C207 (= C135), R208 (≠ V136), G209 (= G137), Y210 (≠ C138), R211 (≠ A139), K212 (≠ Y140), C213 (= C141), S231 (≠ A159)
- binding protoporphyrin ix containing fe: R188 (≠ V117), R208 (≠ V136), Y210 (≠ C138)
- binding iron/sulfur cluster: Q171 (≠ P100), I173 (≠ L102), C174 (= C103), N175 (= N104), H176 (= H105), C177 (= C106), P180 (= P109), C182 (= C111), C217 (= C145), P222 (≠ R150), K233 (= K161), C234 (= C162), A236 (≠ F164), C237 (= C165), T255 (≠ P175), R256 (≠ A176), C257 (= C177), C261 (= C181), V262 (= V182), I265 (≠ A185), R266 (= R186)
Sites not aligning to the query:
- binding iron/sulfur cluster: 34, 35, 36, 37, 38, 40, 44, 48, 59, 60, 62
4v4cB Pyrogallol hydroxytransferase small subunit (see paper)
30% identity, 71% coverage: 45:227/259 of query aligns to 2:191/274 of 4v4cB
- binding calcium ion: I61 (≠ L99), N62 (vs. gap)
- binding iron/sulfur cluster: C13 (= C56), Q14 (≠ I57), D15 (≠ G58), C16 (= C59), N17 (≠ Q60), N18 (≠ A61), C19 (= C62), C23 (= C66), C68 (= C103), M69 (≠ N104), H70 (= H105), C71 (= C106), C76 (= C111), V92 (= V128), C109 (= C145), V113 (≠ A149), C126 (= C162), M128 (≠ F164), C129 (= C165), P143 (= P175), C145 (= C177), C149 (= C181), S151 (≠ A185), V153 (≠ I187), Y154 (≠ I188)
Q8GC87 Formate dehydrogenase subunit beta; FDH subunit beta; Formate dehydrogenase small subunit from Megalodesulfovibrio gigas (strain ATCC 19364 / DSM 1382 / NCIMB 9332 / VKM B-1759) (Desulfovibrio gigas) (see paper)
29% identity, 62% coverage: 48:208/259 of query aligns to 4:184/215 of Q8GC87
- C12 (= C56) binding
- C15 (= C59) binding
- C18 (= C62) binding
- C22 (= C66) binding
- C73 (= C103) binding
- C76 (= C106) binding
- C81 (= C111) binding
- C121 (= C145) binding
- C138 (= C162) binding
- C141 (= C165) binding
- C153 (= C177) binding
- C157 (= C181) binding
1h0hB Tungsten containing formate dehydrogenase from desulfovibrio gigas (see paper)
29% identity, 62% coverage: 48:208/259 of query aligns to 3:183/214 of 1h0hB
- binding iron/sulfur cluster: F4 (≠ M49), C11 (= C56), T12 (≠ I57), A13 (≠ G58), C14 (= C59), R15 (≠ Q60), C17 (= C62), C21 (= C66), H25 (vs. gap), K50 (≠ V81), Q71 (≠ L102), C72 (= C103), R73 (≠ N104), C75 (= C106), P79 (= P110), C80 (= C111), V101 (≠ A139), C120 (= C145), P125 (vs. gap), K136 (= K161), C137 (= C162), D138 (≠ T163), M139 (≠ F164), C140 (= C165), C152 (= C177), C156 (= C181), P157 (≠ V182), A160 (= A185)
Query Sequence
>WP_086508321.1 NCBI__GCF_002151265.1:WP_086508321.1
MDHLRRSLLGQLARLSAGAALIPLSSVASAGINSQPPRREGDPTKRYGMLIDLRQCIGCQ
ACTVSCHIENDAPLGKFRTTVSQYEVEHQETGELATFMLPRLCNHCENPPCVPVCPVQAT
YQQQDGIVVVDSDRCVGCAYCVNACPYDARFINDRTQTADKCTFCAHRLEAGLLPACVES
CVGGARIIGDMRDPESQISRMIAEHRDALMVLQPEKNTLPQVFYLGMDERFITRPLAEPV
ALEVLDPHGKEMGYEFHGH
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory