SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_086508611.1 NCBI__GCF_002151265.1:WP_086508611.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

2xuaH Crystal structure of the enol-lactonase from burkholderia xenovorans lb400 (see paper)
32% identity, 67% coverage: 1:266/399 of query aligns to 1:259/261 of 2xuaH

query
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2xuaH

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active site: L32 (= L30), M98 (≠ I99), D214 (= D220), H241 (= H248)
binding laevulinic acid: L32 (= L30), R135 (= R136), V151 (≠ I152), R154 (= R155), W155 (= W156), Y183 (= Y184), A216 (= A222)

4uhfA Structural studies of a thermophilic esterase from thermogutta terrifontis (l37a mutant with butyrate bound) (see paper)
24% identity, 63% coverage: 24:276/399 of query aligns to 28:277/278 of 4uhfA

query
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active site: F34 (≠ L30), L99 (≠ T97), S100 (= S98), M101 (≠ I99), D124 (≠ N122), E164 (≠ P159), D221 (= D220), H249 (= H248), L250 (≠ V249)
binding butanoic acid: G33 (≠ P29), F34 (≠ L30), S100 (= S98), H249 (= H248)

4uheA Structural studies of a thermophilic esterase from thermogutta terrifontis (malate bound) (see paper)
24% identity, 61% coverage: 24:266/399 of query aligns to 28:267/272 of 4uheA

query
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4uheA

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active site: F34 (≠ L30), L99 (≠ T97), S100 (= S98), M101 (≠ I99), D124 (≠ N122), E164 (≠ P159), D221 (= D220), H249 (= H248), L250 (≠ V249)
binding d-malate: F34 (≠ L30), S100 (= S98), M101 (≠ I99), Y104 (≠ V102), R138 (= R136), H249 (= H248)

4uhdA Structural studies of a thermophilic esterase from thermogutta terrifontis (acetate bound) (see paper)
24% identity, 61% coverage: 24:266/399 of query aligns to 28:267/274 of 4uhdA

query
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4uhdA

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active site: F34 (≠ L30), L99 (≠ T97), S100 (= S98), M101 (≠ I99), D124 (≠ N122), E164 (≠ P159), D221 (= D220), H249 (= H248), L250 (≠ V249)
binding acetate ion: G33 (≠ P29), F34 (≠ L30), S100 (= S98), Y104 (≠ V102), R138 (= R136), H249 (= H248)
binding magnesium ion: A233 (= A232), I236 (≠ C235), S239 (≠ A238)

5z7wB Crystal structure of striga hermonthica htl1 (shhtl1) (see paper)
24% identity, 62% coverage: 17:263/399 of query aligns to 18:263/271 of 5z7wB

query
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5z7wB

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V

E

G

A

A

E

D

P

-

A

-

L

M

K

E

A

S

G

L

W

A

C

V

T

Q

Q

E

M

F

G

S

R

R

G

T

D

L

D

F

E

N

S

M

Y

R

A

P

R

D

L

I

C

A

E

L

M

S

L

I

G

L

R

Q

T

T

D

I

F

F

R

Y

G

S

K

D

L

V

K

R

G

-

P

P

L

L

A

L

E

P

H

H

P

V

D

T

I

V

G

P

V

C

H

H

L

I

L

I

G

Q

G

S

S

V

A

K

D

D

V

L

A

A

T

V

P

P

P

V

E

A

T

V

L

S

Q

E

A

Y

L

I

A

H

A

Q

E

S

C

L

G

G

G

G

-

E

A

S

P

I

L

L

E

E

I

V

L

M

E

A

G

T

I

E

A

G

H

H

V

L

P

P

S

Q

V

L

E

S

A

S

P

P

A

D

A

V

M

V

A

V

K

P

R

V

L

L

L

L

binding magnesium ion: F28 (≠ L30), S97 (= S98), V98 (≠ I99)

6i8wB Crystal structure of a membrane phospholipase a, a novel bacterial virulence factor (see paper)
24% identity, 71% coverage: 4:286/399 of query aligns to 45:309/310 of 6i8wB

query
sites
6i8wB

L

V

N

Q

V

V

D

D

G

N

R

L

S

E

V

I

A

A

Y

Y

R

L

L

E

L

G

G

G

P

S

E

E

A

K

L

N

P

P

L

T

V

L

V

L

L

L

A

I

H

H

P

G

L

F

G

G

M

A

S

D

Q

K

A

D

V
x
N

W

W

D

L

E

R

L

F

L

A

P

R

A

P

L

L

L

T

P

E

R

R

Y

Y

R

H

V

V

L

V

T

A

W

L

D

D

L

L

P

P

G

G

H

F

G

G

A

D

S

S

Q

S

A

K

W

P

P

Q

A

Q

E

A

G

S

G

Y

E

D

I

V

T

G

P

T

A

Q

A

A

-

E

-

R

L

V

A

A

R

N

E

F

A

A

L

A

A

A

L

I

V

-

E

-

H

-

A

-

G

G

V

V

S

R

R

R

F

L

H

H

F

L

V

A

G

G

T

N

S

S

I

M

G

G

G

G

V

H

V

I

G

A

Q

A

Q

L

L

Y

I

A

S

A

E

R

H

H

A

P

E

E

R

Q

L

V

Y

L

S

S

I

L

T

A

L

L

T

I

N

D

T

N

G

A

A

G

V

V

I

M

G

P

-

A

-

R

N

K

A

S

E

E

L

L

W

F

S

E

T

D

R

-

A

L

E

E

R

R

I

G

R

E

Q

N

E

P

G

L

L

V

A

V

A

R

M

Q

A

P

E

E

E

D

I

F

V

-

P

-

R

Q

W

K

F

L

A

L

P

D

A

F

C

V

F

F

E

V

A

Q

E

Q

P

P

A

P

L

L

K

P

A

A

G

P

W

L

C

K

T

R

Q

Y

M

L

G

G

R

E

-

R

-

A

-

V

G

A

D

A

D

S

E

A

S

F

Y

N

A

A

R

Q

L

I

C

F

E

E

M

Q

L

L

G

R

R

Q

T

R

D

Y

F

I

R

P

G

L

K

E

L

P

K

E

G

L

P

P

L

K

A

I

E

E

H

A

P

P

D

T

I

L

G

-

V

-

H

-

L

L

L

L

G

W

G

G

S

D

A

R

D

D

V

R

A

V

T

L

P

D

P

V

E

S

T

S

L

I

Q

E

A

V

L

M

A

R

A

P

E

L

C

L

G

K

G

R

A

P

P

S

L

V

E

V

I

I

L

M

E

E

G

N

I

C

A

G

H

H

V

V

P

P

S

M

V

V

E

E

A

R

P

P

A

-

A

-

M

-

A

-

K

-

R

-

L

-

L

-

L

-

W

-

M

-

A

-

S

-

E

-

R

-

E

E

D

E

V

T

G

A

E

Q

H

H

G

Y

V

Q

S

A

Y

F

A

L

D

D

G

G

L

V

E

R

T

N

binding undecanoic acid: N77 (≠ V36)

Sites not aligning to the query:

binding undecanoic acid: 31

5z89A Structural basis for specific inhibition of highly sensitive shhtl7 receptor (see paper)
22% identity, 63% coverage: 24:275/399 of query aligns to 18:268/268 of 5z89A

query
sites
5z89A

V

V

V

V

L

L

A

G

H

H

P

G

L

Y

G

G

M

T

S

D

Q

Q

A

S

V

V

W

W

D

K

E

L

L

L

L

V

P

P

A

Y

L

L

L

V

P

D

R

D

Y

Y

R

K

V

V

L

L

T

L

W

Y

D

D

L

H

P

M

G

G

H

A

G

G

A

T

S

T

Q

N

A

P

W

D

P

Y

A

F

E

D

G

F

G

D

E

R

I

Y

T

S

P

S

-

L

A

E

A

G

L

Y

A

S

R

Y

E

D

A

L

L

I

A

A

L

I

V

L

E

E

H

E

A

F

G

Q

V

V

S

S

R

K

F

C

H

I

F

Y

V

V

G

G

-

H

-

D

-

M

T

S

S

S

I

M

G

A

G

A

V

A

V

V

G

A

Q

S

Q

I

L

F

I

R

S

P

E

D

H

L

A

F

E

H

R

K

L

L

Y

-

S

-

I

V

T

M

L

I

T

S

N

P

T

T

G

P

A

R

V

L

I

I

G

N

N

T

A

E

E

E

L

Y

W

Y

S

G

T

G

R

F

A

E

E

Q

R

K

I

V

R
x
M

Q

D

E

E

G
x
T

L

L

A

R

A

S

M

L

A

D

E

E

E

N

I

F

-

K

-

S

V

L

P

S

R

L

W

G

F

T

A

A

P

P

-

L

-

L

-

L

A

A

C

C

F

-

E

D

A

L

E

E

P

S

A

A

L

A

K

M

A

Q

G

E

W

Y

C

C

T

R

Q

T

M

L

G

F

R

N

G

M

D

R

D

P

E

D

S

I

Y

A

A

C

R

C

L

I

C
x
T

E

R

M

M

L

I

G

C

R

G

T

L

D

D

F

L

R

R

G

-

K

-

L

-

K

-

G

-

P

P

L

Y

A

L

E

G

H

H

P

V

D

T

I

V

G

P

V

C

H

H

L

I

L

I

G

Q

G

S

S

S

A

N

D

D

V

I

A

M

T

V

P

P

P

V

E

A

T

V

L

G

Q

E

A

Y

L

L

A

R

A

K

E

N

C

L

G

G

G

G

-

P

A

S

P

V

L

V

E

E

I

V

L

M

E

P

G

T

I

E

A

G

H

H

V

L

P

P

S

H

V

L

E

S

A

M

P

P

A

E

A

V

M

T

A

I

K

P

R

V

L

V

L

L

L

R

W

H

M

I

A

-

S

-

E

-

R

R

E

Q

D

D

V

I

G

T

E

D

binding 2-(2-{2-[2-(2-{2-[2-(2-{2-[4-(1,1,3,3-tetramethyl-butyl)-phenoxy]-ethoxy}-ethoxy)-ethoxy]-ethoxy}-ethoxy)-ethoxy]-ethoxy}-ethoxy)-ethanol: M137 (≠ R141), T140 (≠ G144), T188 (≠ C188)

7c8lA Hybrid designing of potent inhibitors of striga strigolactone receptor shhtl7 (see paper)
22% identity, 63% coverage: 24:275/399 of query aligns to 18:268/268 of 7c8lA

query
sites
7c8lA

V

V

V

V

L

L

A

G

H

H

P

G

L

Y

G

G

M

T

S

D

Q

Q

A

S

V

V

W

W

D

K

E

L

L

L

L

V

P

P

A

Y

L

L

L

V

P

D

R

D

Y

Y

R

K

V

V

L

L

T

L

W

Y

D

D

L

H

P

M

G

G

H

A

G

G

A

T

S

T

Q

N

A

P

W

D

P

Y

A

F

E

D

G

F

G

D

E

R

I

Y

T

S

P

S

-

L

A

E

A

G

L

Y

A

S

R

Y

E

D

A

L

L

I

A

A

L

I

V

L

E

E

H

E

A

F

G

Q

V

V

S

S

R

K

F

C

H

I

F

Y

V

V

G

G

-

H

-
x
S

-
x
M

T

S

S

S

I

M

G

A

G

A

V

A

V

V

G

A

Q

S

Q

I

L

F

I

R

S

P

E

D

H

L

A

F

E

H

R

K

L

L

Y

-

S

-

I

V

T

M

L

I

T

S

N

P

T
|
T

G

P

A

R

V

L

I

I

G

N

N

T

A

E

E

E

L

Y

W

Y

S

G

T

G

R
x
F

A

E

E

Q

R

K

I

V

R
x
M

Q

D

E

E

G
x
T

L

L

A

R

A

S

M

L

A

D

E

E

E

N

I

F

-

K

-

S

V

L

P

S

R

L

W

G

F

T

A

A

P

P

-

L

-

L

-

L

A

A

C

C

F

-

E

D

A

L

E

E

P

S

A

A

L

A

K

M

A

Q

G

E

W

Y

C

C

T

R

Q

T

M

L

G

F

R

N

G

M

D

R

D

P

E

D

S

I

Y

A

A

C

R

C

L

I

C

T

E

R

M

M

L

I

G

C

R

G

T
x
L

D

D

F

L

R

R

G

-

K

-

L

-

K

-

G

-

P

P

L

Y

A

L

E

G

H

H

P

V

D

T

I

V

G

P

V

C

H

H

L

I

L

I

G

Q

G

S

S

S

A

N

D

D

V

I

A

M

T

V

P

P

P

V

E

A

T

V

L

G

Q

E

A

Y

L

L

A

R

A

K

E

N

C

L

G

G

G

G

-

P

A

S

P

V

L

V

E

E

I

V

L

M

E

P

G

T

I

E

A

G

H
|
H

V

L

P

P

S

H

V

L

E

S

A

M

P

P

A

E

A

V

M

T

A

I

K

P

R

V

L

V

L

L

L

R

W

H

M

I

A

-

S

-

E

-

R

R

E

Q

D

D

V

I

G

T

E

D

binding 2-(2-{2-[2-(2-{2-[2-(2-{2-[4-(1,1,3,3-tetramethyl-butyl)-phenoxy]-ethoxy}-ethoxy)-ethoxy]-ethoxy}-ethoxy)-ethoxy]-ethoxy}-ethoxy)-ethanol: F132 (≠ R136), M137 (≠ R141), T140 (≠ G144)
binding pyrrolidine-1-carbaldehyde: S93 (vs. gap), M94 (vs. gap), T119 (= T123), L194 (≠ T194), H244 (= H248)

7snuA Crystal structure of shhtl7 from striga hermonthica in complex with strigolactone antagonist rg6 (see paper)
23% identity, 58% coverage: 24:255/399 of query aligns to 21:254/270 of 7snuA

query
sites
7snuA

V

V

V

V

L

L

A

G

H

H

P

G

L

Y

G

G

M

T

S

D

Q

Q

A

S

V

V

W

W

D

K

E

L

L

L

L

V

P

P

A

Y

L

L

L

V

P

D

R

D

Y

Y

R

K

V

V

L

L

T

L

W

Y

D

D

L

H

P

M

G

G

H

A

G

G

A

T

S

T

Q

N

A

P

W

D

P

Y

A

F

E

D

G

F

G

D

E

R

I

Y

T

S

P

S

-

L

A

E

A

G

L

Y

A

S

R

Y

E

D

A

L

L

I

A

A

L

I

V

L

E

E

H

E

A

F

G

Q

V

V

S

S

R

K

F

C

H

I

F

Y

V

V

G

G

-

H

-
x
S

-

M

T

S

S

S

I

M

G

A

G

A

V

A

V

V

G

A

Q

S

Q

I

L

F

I

R

S

P

E

D

H

L

A

F

E

H

R

K

L

L

Y

-

S

-

I

V

T

M

L

I

T

S

N

P

T
|
T

G

P

A

R

V
x
L

I

I

G

N

N

T

A

E

E

E

L

Y

W

Y

S

G

T

G

R
x
F

A

E

E

Q

R

K

I

V

R
x
M

Q

D

E

E

G

T

L

L

A

R

A

S

M

L

A

D

E

E

E

N

I

F

-

K

-

S

V
x
L

P

S

R

L

W

G

F
x
T

A

A

P

P

-

L

-

L

-

L

A

A

C

C

F

-

E

D

A

L

E

E

P

S

A

A

L

A

K

M

A

Q

G

E

W

Y

C

C

T

R

Q

T

M

L

G

F

R

N

G

M

D

R

D

P

E

D

S

I

Y

A

A

C

R

C

L

I

C

T

E

R

M

M

L
x
I

G

C

R

G

T

L

D

D

F

L

R

R

G

-

K

-

L

-

K

-

G

-

P

P

L

Y

A

L

E

G

H

H

P

V

D

T

I

V

G

P

V

C

H

H

L

I

L

I

G

Q

G

S

S

S

A

N

D

D

V

I

A
x
M

T

V

P

P

P

V

E

A

T

V

L

G

Q

E

A

Y

L

L

A

R

A

K

E

N

C

L

G

G

G

G

-

P

A

S

P

V

L

V

E

E

I

V

L

M

E

P

G

T

I

E

A

G

H

H

V

L

P

P

S

H

V

L

E

S

A

M

P

P

binding 2-{(2S)-1-[(4-ethoxyphenyl)methyl]-4-[(2E)-3-(4-methoxyphenyl)prop-2-en-1-yl]piperazin-2-yl}ethan-1-ol: S96 (vs. gap), T122 (= T123), L125 (≠ V126), F135 (≠ R136), M140 (≠ R141), L154 (≠ V153), T158 (≠ F157), I194 (≠ L191), M220 (≠ A222)

5z7yA Crystal structure of striga hermonthica htl7 (shhtl7) (see paper)
23% identity, 58% coverage: 24:255/399 of query aligns to 19:252/267 of 5z7yA

query
sites
5z7yA

V

V

V

V

L

L

A

G

H

H

P

G

L
x
Y

G

G

M

T

S

D

Q

Q

A

S

V

V

W

W

D

K

E

L

L

L

L

V

P

P

A

Y

L

L

L

V

P

D

R

D

Y

Y

R

K

V

V

L

L

T

L

W

Y

D

D

L

H

P

M

G

G

H

A

G

G

A

T

S

T

Q

N

A

P

W

D

P

Y

A

F

E

D

G

F

G

D

E

R

I

Y

T

S

P

S

-

L

A

E

A

G

L

Y

A

S

R

Y

E

D

A

L

L

I

A

A

L

I

V

L

E

E

H

E

A

F

G

Q

V

V

S

S

R

K

F

C

H

I

F

Y

V

V

G

G

-

H

-
x
S

-
x
M

T

S

S

S

I

M

G

A

G

A

V

A

V

V

G

A

Q

S

Q

I

L

F

I

R

S

P

E

D

H

L

A

F

E

H

R

K

L

L

Y

-

S

-

I

V

T

M

L

I

T

S

N

P

T

T

G

P

A

R

V

L

I

I

G

N

N

T

A

E

E

E

L

Y

W

Y

S

G

T

G

R

F

A

E

E

Q

R

K

I

V

R

M

Q

D

E

E

G

T

L

L

A

R

A

S

M

L

A

D

E

E

E

N

I

F

-

K

-

S

V

L

P

S

R

L

W

G

F

T

A

A

P

P

-

L

-

L

-

L

A

A

C

C

F

-

E

D

A

L

E

E

P

S

A

A

L

A

K

M

A

Q

G

E

W

Y

C

C

T

R

Q

T

M

L

G

F

R

N

G

M

D

R

D

P

E

D

S

I

Y

A

A

C

R

C

L

I

C

T

E

R

M

M

L

I

G

C

R

G

T

L

D

D

F

L

R

R

G

-

K

-

L

-

K

-

G

-

P

P

L

Y

A

L

E

G

H

H

P

V

D

T

I

V

G

P

V

C

H

H

L

I

L

I

G

Q

G

S

S

S

A

N

D

D

V

I

A

M

T

V

P

P

P

V

E

A

T

V

L

G

Q

E

A

Y

L

L

A

R

A

K

E

N

C

L

G

G

G

G

-

P

A

S

P

V

L

V

E

E

I

V

L

M

E

P

G

T

I

E

A

G

H

H

V

L

P

P

S

H

V

L

E

S

A

M

P

P

binding magnesium ion: Y25 (≠ L30), S94 (vs. gap), M95 (vs. gap)

Q9SQR3 Strigolactone esterase D14; Protein DWARF 14; AtD14; EC 3.1.-.- from Arabidopsis thaliana (Mouse-ear cress) (see 4 papers)
24% identity, 60% coverage: 23:262/399 of query aligns to 21:261/267 of Q9SQR3

query
sites
Q9SQR3

L

I

V

L

V

F

L

L

A

A

H

H

P

G

L

F

G

G

M

T

S

D

Q

Q

A

S

V

A

W

W

D

H

E

L

L

I

L

L

P

P

A

Y

L

F

L

T

P

Q

R

N

Y

Y

R

R

V

V

L

V

T

L

W

Y

D

D

L

L

P

V

G

C

H

A

G

G

A

S

S

V

Q

N

A

P

W

D

P

Y

A

F

E

D

G

F

G

N

E

R

I

Y

T

T

P

T

-

L

A

D

A

P

L

Y

A

V

R

D

E

D

A

L

L

L

A

N

L

I

V

V

E

D

H

S

A

L

G

G

V

I

S

Q

R

N

F

C

H

A

F

Y

V

V

G

G

T

H

S
|
S

I

V

G

S

G

A

V

M

V

I

G

G

Q

I

Q

I

L

A

I

S

S

I

E

R

H

R

A

P

E

E

R

L

L

F

Y

S

S

K

I

L

T

I

L

L

T

I

N

G

T

F

G

S

A

P

V

R

I

F

G

L

N

N

A

D

E

E

L

D

W

Y

S

H

T

G

R

G

A

F

E

E

R

E

I

G

R

E

Q

I

E

E

G

K

L

V

A

F

A

S

M

A

A

M

E

E

E

A

I

N

V

Y

P

E

R

A

W

W

-

V

-

H

-
x
G

F

F

A

A

P

P

A

L

C

A

F

V

E

G

A

A

E

D

-

V

P
|
P

A

A

L

-

K

-

A

-

G

-

W

A

C

V

T

R

Q

E

M

F

G

S

R

R

G

T

D

L

D

F

E

N

S

M

Y

R

A

P

R

D

L

I

C

S

E

L

M

F

L

V

G

S

R

R

T

T

D

V

F

F

R

N

G

S

K

D

L

L

K

R

G

G

P

V

L

L

A

G

E

L

H

-

P

V

D

R

I

V

G

P

V

T

H

C

L

V

L

I

G

Q

G

T

S

A

A

K

D

D

V

V

A

S

T

V

P

P

P

A

E

S

T

V

L

A

Q

E

A

Y

L

L

A

R

A

S

E

H

C

L

G

G

G

G

-

D

A

T

P

T

L

V

E

E

I

T

L

L

E

K

G

T

I

E

A

G

H

H

V

L

P

P

S

Q

V

L

E

S

A

A

P

P

A

A

A

Q

M

L

A

A

K

Q

R

F

L

L

S97 (= S98) active site, Nucleophile; mutation to A: Loss of activity.
G158 (vs. gap) mutation to E: Loss of interaction with MAX2.
P169 (= P166) mutation to L: In d14-seto; loss of activity.

4b9eA Structure of a putative epoxide hydrolase from pseudomonas aeruginosa, with bound mfa. (see paper)
30% identity, 27% coverage: 22:129/399 of query aligns to 28:135/297 of 4b9eA

query
sites
4b9eA

P

P

L

P

V

L

V

L

L

L

A

L

H

H

P

G

L
x
Y

G

P

M
x
Q

S

T

Q

H

A

L

V

A

W

W

D

H

E

R

L

I

L

A

P

P

A

R

L

L

L

A

P

E

R

D

Y

Y

R

S

V

V

L

V

T

L

W

A

D

D

L

L

P

R

G

G

H

Y

G
|
G

A
x
E

S
|
S

Q
x
R

A

A

W

L

P

D

A

E

E

E

G

G

G

A

E

D

I

Y

T

S

P

K

A

A

A

A

L

L

A

A

R

R

E

D

A

Q

L

L

A

E

L

T

V

M

E

G

H

Q

A

L

G

G

V

F

S

E

R

R

F

F

H

A

F

V

V

I

G

G

T
x
H

S
x
D

I
x
R

G

G

G

A

V

R

V

V

G

G

Q

Y

Q

R

L

L

I

A

S

L

E

D

H

H

A

P

E

Q

R

A

L

V

Y

A

S

A

I

F

T

V

L

S

T

L

N
x
T

T

V

G

V

A

P

V

I

I

L

G

D

N

N

active site: Y36 (≠ L30), Q38 (≠ M32), H103 (≠ T97), D104 (≠ S98), R105 (≠ I99), T128 (≠ N122)
binding fluoroacetic acid: G65 (= G59), E66 (≠ A60), S67 (= S61), R68 (≠ Q62)

Sites not aligning to the query:

active site: 149, 211, 244, 271
binding fluoroacetic acid: 149, 150, 200, 203, 209, 212, 271

5dnuA Crystal structure of striga kai2-like protein in complex with karrikin (see paper)
23% identity, 62% coverage: 24:269/399 of query aligns to 18:265/267 of 5dnuA

query
sites
5dnuA

V

V

V

V

L

L

A

G

H

H

P

G

L
x
F

G

G

M

T

S

D

Q

Q

A

S

V

V

W

W

D

K

E

H

L

L

L

V

P

P

A

Y

L

L

L

V

P

D

R

S

Y

Y

R

R

V

V

L

L

T

L

W

Y

D

D

L

N

P

M

G

G

H

A

G

G

A

S

S

T

Q

N

A

P

-

E

W

Y

P

F

A

H

E

F

G

E

G

R

E

Y

I

S

T

T

P

L

A

Q

A

G

L

Y

A

A

R

H

E

D

A

L

L

L

A

V

L

I

V

L

E

H

H

E

A

F

G

N

V

I

S

R

R

S

F

C

H

I

F

F

V

V

G

G

T

H

S
|
S

I
x
L

G

S

G

A

V

M

V

T

G

G

Q

A

Q

I

L

A

I

S

S

I

E

I

H

R

A

P

E

D

R

L

L

F

Y

Q

S

K

I

I

T

V

L

M

T

L

N

S

T

A

G

S

A

P

V

R

I
x
F

G

L

N

N

A

T

E

A

L

D

W

Y

S

L

T

G

R

G

A

F

E

E

R

P

I

A

R

D

Q

V

E

E

G

Q

L
|
L

A

A

A

G

M

A

A

I

E

E

E

A

I

N

V

Y

P

K

R

S

W

W

-

V

-

S

-

G

F
|
F

A

A

P

P

A

M

C

V

F

V

E

G

A

G

E

D

P

-

A

-

L

M

K

D

A

S

G

V

W

A

C

V

T

Q

Q

E

M

F

G

S

R

R

G

T

D

L

D

F

E

N

S

M

Y

R

A

P

R

D

L

I

C

A

E

R

M

S

L

V

G

F

R

R

T

T

D
x
I

F
|
F

R

T

G

S

K

D

L

L

K

R

G

D

P

Y

L

L

A

G

E

R

H

-

P

V

D

T

I

V

G

P

V

C

H

H

L

I

L

I

G

Q

G

S

S

S

A
x
R

D

D

V

M

A
|
A

T

V

P

P

P

V

E

S

T

V

L

A

Q

G

A

Y

L

I

A

H

A

N

E

R

C

V

G

G

G

G

-

R

A

S

P

V

L

V

E

E

I

V

L

M

E

N

G

T

I

E

A
x
G

H
|
H

V

L

P

P

S

Q

V

L

E

S

A

A

P

P

A

E

A

V

M

A

A

I

K

P

R

V

L

L

L

L

L

R

W

H

M

I

A

K

S

N

E

D

active site: F24 (≠ L30), S93 (= S98), L94 (≠ I99), R214 (≠ A219), G243 (≠ A247)
binding 3-methyl-2H-furo[2,3-c]pyran-2-one: F24 (≠ L30), F122 (≠ I127), L140 (= L145), F155 (= F157), I191 (≠ D195), F192 (= F196), A217 (= A222), H244 (= H248)

7p4kA Soluble epoxide hydrolase in complex with fl217 (see paper)
30% identity, 28% coverage: 22:132/399 of query aligns to 31:141/313 of 7p4kA

query
sites
7p4kA

P

P

L

A

V

V

V

C

L

L

A

C

H

H

P

G

L
x
F

G

P

M

E

S

S

Q

W

A

Y

V

S

W

W

D

R

E

Y

L

Q

L

I

P

P

A

A

L

L

L

A

P

Q

R

A

-

G

Y

Y

R

R

V

V

L

L

T

A

W

M

D

D

L

M

P

K

G

G

H

Y

G

G

A

E

S

S

Q

S

A

A

W

-

P

P

A

P

E

E

G

I

G

E

E

E

I

Y

T

C

P

M

A

E

A

V

L

L

A

C

R

K

E

E

A

M

L

V

A

T

L

F

V

L

E

D

H

K

A

L

G

G

V

L

S

S

R

Q

F

A

H

V

F

F

V

I

G

G

T

H

S
x
D

I
x
W

G

G

G

G

V
x
M

V

L

G

V

Q

W

Q

Y

L

M

I

A

S

L

E

F

H

Y

A

P

E

E

R

R

L

V

Y

R

S

A

I

V

T

A

L

S

T

L

N

N

T

T

G
x
P

A

F

V

I

I

P

G

A

N

N

A

P

E

N

L

M

binding ~{N}-[[4-(cyclopropylsulfonylamino)-2-(trifluoromethyl)phenyl]methyl]-1-[(3-fluorophenyl)methyl]indole-5-carboxamide: F39 (≠ L30), D107 (≠ S98), W108 (≠ I99), M111 (≠ V102), P133 (≠ G124)

Sites not aligning to the query:

binding ~{N}-[[4-(cyclopropylsulfonylamino)-2-(trifluoromethyl)phenyl]methyl]-1-[(3-fluorophenyl)methyl]indole-5-carboxamide: 149, 185, 232, 264, 269, 290

8qn0A Soluble epoxide hydrolase in complex with rk3 (see paper)
30% identity, 28% coverage: 22:132/399 of query aligns to 34:144/322 of 8qn0A

query
sites
8qn0A

P

P

L

A

V

V

V

C

L

L

A

C

H

H

P

G

L

F

G

P

M

E

S

S

Q

W

A

Y

V

S

W

W

D

R

E

Y

L

Q

L

I

P

P

A

A

L

L

L

A

P

Q

R

A

-

G

Y

Y

R

R

V

V

L

L

T

A

W

M

D

D

L

M

P

K

G

G

H

Y

G

G

A

E

S

S

Q

S

A

A

W

-

P

P

A

P

E

E

G

I

G

E

E

E

I

Y

T

C

P

M

A

E

A

V

L

L

A

C

R

K

E

E

A

M

L

V

A

T

L

F

V

L

E

D

H

K

A

L

G

G

V

L

S

S

R

Q

F

A

H

V

F

F

V

I

G

G

T

H

S
x
D

I
x
W

G

G

G

G

V

M

V

L

G

V

Q

W

Q

Y

L

M

I

A

S

L

E

F

H

Y

A

P

E

E

R

R

L

V

Y

R

S

A

I

V

T

A

L

S

T

L

N

N

T

T

G

P

A

F

V

I

I

P

G

A

N

N

A

P

E

N

L

M

binding (3~{a}~{R},6~{a}~{S})-~{N}-[(2,4-dichlorophenyl)methyl]-5-(4-methylphenyl)sulfonyl-1,3,3~{a},4,6,6~{a}-hexahydropyrrolo[3,4-c]pyrrole-2-carboxamide: D110 (≠ S98), W111 (≠ I99)

Sites not aligning to the query:

binding (3~{a}~{R},6~{a}~{S})-~{N}-[(2,4-dichlorophenyl)methyl]-5-(4-methylphenyl)sulfonyl-1,3,3~{a},4,6,6~{a}-hexahydropyrrolo[3,4-c]pyrrole-2-carboxamide: 158, 194, 241, 273, 274, 299

8qzdA Soluble epoxide hydrolase in complex with epoxykinin (see paper)
30% identity, 28% coverage: 22:132/399 of query aligns to 32:142/320 of 8qzdA

query
sites
8qzdA

P

P

L

A

V

V

V

C

L

L

A

C

H

H

P

G

L

F

G

P

M

E

S

S

Q

W

A

Y

V

S

W

W

D

R

E

Y

L

Q

L

I

P

P

A

A

L

L

L

A

P

Q

R

A

-

G

Y

Y

R

R

V

V

L

L

T

A

W

M

D

D

L

M

P

K

G

G

H

Y

G

G

A

E

S

S

Q

S

A

A

W

-

P

P

A

P

E

E

G

I

G

E

E

E

I

Y

T

C

P

M

A

E

A

V

L

L

A

C

R

K

E

E

A

M

L

V

A

T

L

F

V

L

E

D

H

K

A

L

G

G

V

L

S

S

R

Q

F

A

H

V

F

F

V

I

G

G

T

H

S
x
D

I
x
W

G

G

G

G

V

M

V

L

G

V

Q

W

Q

Y

L

M

I

A

S

L

E

F

H

Y

A

P

E

E

R

R

L

V

Y

R

S

A

I

V

T

A

L

S

T

L

N

N

T

T

G

P

A

F

V

I

I

P

G

A

N

N

A

P

E

N

L

M

binding 2-[5-bromanyl-3-[2,2,2-tris(fluoranyl)ethanoyl]indol-1-yl]-N-cycloheptyl-ethanamide: D108 (≠ S98), W109 (≠ I99)

Sites not aligning to the query:

binding 2-[5-bromanyl-3-[2,2,2-tris(fluoranyl)ethanoyl]indol-1-yl]-N-cycloheptyl-ethanamide: 156, 160, 181, 192, 201, 239, 271, 272, 297

8qmzA Soluble epoxide hydrolase in complex with rk4 (see paper)
30% identity, 28% coverage: 22:132/399 of query aligns to 32:142/320 of 8qmzA

query
sites
8qmzA

P

P

L

A

V

V

V

C

L

L

A

C

H

H

P

G

L

F

G

P

M

E

S

S

Q

W

A

Y

V

S

W

W

D

R

E

Y

L

Q

L

I

P

P

A

A

L

L

L

A

P

Q

R

A

-

G

Y

Y

R

R

V

V

L

L

T

A

W

M

D

D

L

M

P

K

G

G

H

Y

G

G

A

E

S

S

Q

S

A

A

W

-

P

P

A

P

E

E

G

I

G

E

E

E

I

Y

T

C

P

M

A

E

A

V

L

L

A

C

R

K

E

E

A

M

L

V

A

T

L

F

V

L

E

D

H

K

A

L

G

G

V

L

S

S

R

Q

F

A

H

V

F

F

V

I

G

G

T

H

S
x
D

I
x
W

G

G

G

G

V
x
M

V

L

G

V

Q

W

Q

Y

L

M

I

A

S

L

E

F

H

Y

A

P

E

E

R

R

L

V

Y

R

S

A

I

V

T

A

L

S

T

L

N

N

T

T

G

P

A

F

V

I

I

P

G

A

N

N

A

P

E

N

L

M

binding (3~{a}~{R},6~{a}~{S})-~{N}-[(2,4-dichlorophenyl)methyl]-2-(4-methylphenyl)sulfonyl-3,3~{a},4,5,6,6~{a}-hexahydro-1~{H}-cyclopenta[c]pyrrole-5-carboxamide: D108 (≠ S98), W109 (≠ I99), M112 (≠ V102)

Sites not aligning to the query:

binding (3~{a}~{R},6~{a}~{S})-~{N}-[(2,4-dichlorophenyl)methyl]-2-(4-methylphenyl)sulfonyl-3,3~{a},4,5,6,6~{a}-hexahydro-1~{H}-cyclopenta[c]pyrrole-5-carboxamide: 156, 157, 192, 239, 271, 272, 297

7ebaA Co-crystal of kurarinone with seh (see paper)
30% identity, 28% coverage: 22:132/399 of query aligns to 29:139/316 of 7ebaA

query
sites
7ebaA

P

P

L

A

V

V

V

C

L

L

A

C

H

H

P

G

L

F

G

P

M

E

S

S

Q

W

A

Y

V

S

W

W

D

R

E

Y

L

Q

L

I

P

P

A

A

L

L

L

A

P

Q

R

A

-

G

Y

Y

R

R

V

V

L

L

T

A

W

M

D

D

L

M

P

K

G

G

H

Y

G

G

A

E

S

S

Q

S

A

A

W

-

P

P

A

P

E

E

G

I

G

E

E

E

I

Y

T

C

P

M

A

E

A

V

L

L

A

C

R

K

E

E

A

M

L

V

A

T

L

F

V

L

E

D

H

K

A

L

G

G

V

L

S

S

R

Q

F

A

H

V

F

F

V

I

G

G

T

H

S
x
D

I
x
W

G

G

G

G

V
x
M

V

L

G

V

Q

W

Q
x
Y

L

M

I

A

S

L

E

F

H

Y

A

P

E

E

R

R

L

V

Y

R

S

A

I

V

T

A

L

S

T

L

N

N

T

T

G
x
P

A

F

V
x
I

I

P

G
x
A

N

N

A

P

E

N

L

M

binding (2S)-2-[2,4-bis(oxidanyl)phenyl]-5-methoxy-8-[(2R)-5-methyl-2-prop-1-en-2-yl-hex-4-enyl]-7-oxidanyl-2,3-dihydrochromen-4-one: D105 (≠ S98), W106 (≠ I99), M109 (≠ V102), Y113 (≠ Q106), P131 (≠ G124), I133 (≠ V126), A135 (≠ G128)

Sites not aligning to the query:

binding (2S)-2-[2,4-bis(oxidanyl)phenyl]-5-methoxy-8-[(2R)-5-methyl-2-prop-1-en-2-yl-hex-4-enyl]-7-oxidanyl-2,3-dihydrochromen-4-one: 151, 154, 236, 239, 242

6i5gA X-ray structure of human soluble epoxide hydrolasE C-terminal domain (hseh ctd)in complex with 15d-pgj2 (see paper)
30% identity, 28% coverage: 22:132/399 of query aligns to 30:140/316 of 6i5gA

query
sites
6i5gA

P

P

L

A

V

V

V

C

L

L

A

C

H

H

P

G

L
x
F

G

P

M

E

S

S

Q

W

A

Y

V

S

W

W

D

R

E

Y

L

Q

L

I

P

P

A

A

L

L

L

A

P

Q

R

A

-

G

Y

Y

R

R

V

V

L

L

T

A

W

M

D

D

L

M

P

K

G

G

H

Y

G

G

A

E

S

S

Q

S

A

A

W

-

P

P

A

P

E

E

G

I

G

E

E

E

I

Y

T

C

P

M

A

E

A

V

L

L

A

C

R

K

E

E

A

M

L

V

A

T

L

F

V

L

E

D

H

K

A

L

G

G

V

L

S

S

R

Q

F

A

H

V

F

F

V

I

G

G

T

H

S
x
D

I
x
W

G

G

G

G

V

M

V

L

G

V

Q

W

Q

Y

L

M

I

A

S

L

E

F

H

Y

A

P

E

E

R

R

L

V

Y

R

S

A

I

V

T

A

L

S

T

L

N

N

T

T

G

P

A

F

V

I

I

P

G

A

N

N

A

P

E

N

L

M

active site: F38 (≠ L30), D106 (≠ S98), W107 (≠ I99)

Sites not aligning to the query:

active site: 267, 295
binding (5E,14E)-11-oxoprosta-5,9,12,14-tetraen-1-oic acid: 158, 186, 188, 190, 267, 268, 269, 295, 296

6yl4A Soluble epoxide hydrolase in complex with 3-((r)-3-(1-hydroxyureido) but-1-yn-1-yl)-n-((s)-3-phenyl-3-(4-trifluoromethoxy)phenyl)propyl) benzamide (see paper)
30% identity, 28% coverage: 22:132/399 of query aligns to 31:141/319 of 6yl4A

query
sites
6yl4A

P

P

L

A

V

V

V

C

L

L

A

C

H

H

P

G

L
x
F

G

P

M

E

S

S

Q

W

A

Y

V

S

W

W

D

R

E

Y

L

Q

L

I

P

P

A

A

L

L

L

A

P

Q

R

A

-

G

Y

Y

R

R

V

V

L

L

T

A

W

M

D

D

L

M

P

K

G

G

H

Y

G

G

A

E

S

S

Q

S

A

A

W

-

P

P

A

P

E

E

G

I

G

E

E

E

I

Y

T

C

P

M

A

E

A

V

L

L

A

C

R

K

E

E

A

M

L

V

A

T

L

F

V

L

E

D

H

K

A

L

G

G

V

L

S

S

R

Q

F

A

H

V

F

F

V

I

G

G

T

H

S
x
D

I
x
W

G

G

G

G

V
x
M

V

L

G

V

Q

W

Q

Y

L

M

I

A

S

L

E

F

H

Y

A

P

E

E

R

R

L

V

Y

R

S

A

I

V

T

A

L

S

T

L

N

N

T

T

G

P

A

F

V

I

I

P

G

A

N

N

A

P

E

N

L

M

active site: F39 (≠ L30), D107 (≠ S98), W108 (≠ I99)
binding 3-[(3~{R})-3-[aminocarbonyl(oxidanyl)amino]but-1-ynyl]-~{N}-[(3~{S})-3-phenyl-3-[4-(trifluoromethyloxy)phenyl]propyl]benzamide: D107 (≠ S98), W108 (≠ I99), M111 (≠ V102)

Sites not aligning to the query:

active site: 268, 296
binding 3-[(3~{R})-3-[aminocarbonyl(oxidanyl)amino]but-1-ynyl]-~{N}-[(3~{S})-3-phenyl-3-[4-(trifluoromethyloxy)phenyl]propyl]benzamide: 147, 155, 156, 180, 191, 238, 241, 269, 270, 296, 297

Query Sequence

>WP_086508611.1 NCBI__GCF_002151265.1:WP_086508611.1
MAFLNVDGRSVAYRLLGPEALPLVVLAHPLGMSQAVWDELLPALLPRYRVLTWDLPGHGA
SQAWPAEGGEITPAALAREALALVEHAGVSRFHFVGTSIGGVVGQQLISEHAERLYSITL
TNTGAVIGNAELWSTRAERIRQEGLAAMAEEIVPRWFAPACFEAEPALKAGWCTQMGRGD
DESYARLCEMLGRTDFRGKLKGPLAEHPDIGVHLLGGSADVATPPETLQALAAECGGAPL
EILEGIAHVPSVEAPAAMAKRLLLWMASEREDVGEHGVSYADGLETRKQVLGEEHVARAS
RNANSLDAPFQQMITRLAWGELWSNDDLTRRERSLITTGILAALGREELVLHLKTAKRIG
LSEAELRQVLMHVAIYAGVPAANHAFALAKELGWGEELG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory