SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_086508957.1 NCBI__GCF_002151265.1:WP_086508957.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 8 hits to proteins with known functional sites (download)

P48636 Cytokinin riboside 5'-monophosphate phosphoribohydrolase; AMP nucleosidase; PaLOG; EC 3.2.2.n1; EC 3.2.2.4 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
53% identity, 98% coverage: 1:182/185 of query aligns to 3:184/195 of P48636

query
sites
P48636

M

L

S

R

N

S

F

V

C

C

V

V

Y

F

L

C

G

G

S

A

R

S

D

P

G

G

R

A

D

S

P

P

T

V

F

Y

L

Q

E

E

A

A

T

A

R

V

A

A

L

L

G

G

H

R

E

H

M

L

A

A

A

E

R

R

G

G

H

L

G

T

L

L

V

V

Y

Y

G

G

A

G

R

A

I

V

G

G

L

L

M

M

G

G

A

T

L

V

A

A

N

D

A

A

V

A

L

L

E

A

R

A

G

G

N

E

V

V

I

I

G

G

V

I

M

I

P

P

D

Q

H

S

L

L

V

Q

E

E

R

A

E
|
E

Q

I

A

G

H

H

E

K

G

G

L

L

P

T

T

R

L

L

I

E

R

V

V

V

N

D

N

G

M
|
M

H

H

E

A

R
|
R

K
|
K

A

A

S

R

M

M

A

A

A

E

H

L

A

A

D

D

A

A

F

F

I

I

A

A

L

L

P

P

G

G

G
|
G

I
x
L

G
|
G

T
|
T

M
x
L

E
|
E

L

L

F

F

E
|
E

A

V

W

W

T

T

W

W

Q

G

Y

Q

L

L

G

G

L

Y

H

H

D

A

K

K

P

P

I

L

G

G

V

L

L

L

D

E

I

V

A

N

G

G

F

F

Y

Y

S

D

P

P

L

L

A

T

F

F

L

L

D

D

S

H

T

L

V

V

E

D

H

E

G

R

F

F

L

V

N

R

G

A

R

E

T

H

R

R

D

G

S

M

L

L

I

Q

D

R

A

G

A

A

E

S

P

P

A

E

A

A

L

L

D

D

A

A

L

L

E

A

A

A

R

W

L

T

A

P

S

S

E74 (= E72) binding substrate
M89 (= M87) mutation to A: Almost complete loss of activity.
R92 (= R90) mutation to A: Almost complete loss of activity.
RK 92:93 (= RK 90:91) binding substrate
109:115 (vs. 107:113, 71% identical) binding substrate
T112 (= T110) mutation to A: Almost complete loss of activity.
E114 (= E112) mutation to A: Almost complete loss of activity.
E115 (= E113) mutation to A: Almost complete loss of activity.
E118 (= E116) mutation to A: Retains 8% of wild-type activity.

5zbkA Crystal structure of type-i log from pseudomonas aeruginosa pao1 in complex with amp (see paper)
53% identity, 96% coverage: 1:178/185 of query aligns to 2:179/182 of 5zbkA

query
sites
5zbkA

M

L

S

R

N

S

F

V

C

C

V

V

Y

F

L

C

G

G

S
x
A

R

S

D

P

G

G

R

A

D

S

P

P

T

V

F

Y

L

Q

E

E

A

A

T

A

R

V

A

A

L

L

G

G

H

R

E

H

M

L

A

A

A

E

R

R

G

G

H

L

G

T

L

L

V

V

Y

Y

G

G

A

G

R

A

I

V

G

G

L

L

M

M

G

G

A

T

L

V

A

A

N

D

A

A

V

A

L

L

E

A

R

A

G

G

N

E

V

V

I

I

G

G

V

I

M

I

P

P

D

Q

H

S

L

L

V

Q

E

E

R

A

E
|
E

Q

I

A

G

H

H

E

K

G

G

L

L

P

T

T

R

L

L

I

E

R

V

V

V

N

D

N

G

M
|
M

H

H

E

A

R
|
R

K
|
K

A

A

S

R

M

M

A

A

A

E

H

L

A

A

D

D

A

A

F

F

I

I

A

A

L

L

P

P

G

G

G
|
G

I

L

G
|
G

T
|
T

M

L

E

E

E

A

L

L

F

F

E

E

A

V

W

W

T

T

W

W

Q

G

Y

Q

L

L

G

G

L

Y

H

H

D

A

K

K

P

P

I

L

G

G

V

L

L

L

D

E

I

V

A

N

G

G

F

F

Y

Y

S

D

P

P

L

L

A

T

F

F

L

L

D

D

S

H

T

L

V

V

E

D

H

E

G

R

F

F

L

V

N

R

G

A

R

E

T

H

R

R

D

G

S

M

L

L

I

Q

D

R

A

G

A

A

E

S

P

P

A

E

A

A

L

L

D

D

A

A

L

L

E

A

A

A

binding adenosine monophosphate: A11 (≠ S10), E73 (= E72), M88 (= M87), R91 (= R90), K92 (= K91), G108 (= G107), G110 (= G109), T111 (= T110)

Q5ZC82 Cytokinin riboside 5'-monophosphate phosphoribohydrolase LOG; Protein LONELY GUY; EC 3.2.2.n1 from Oryza sativa subsp. japonica (Rice) (see paper)
49% identity, 95% coverage: 3:177/185 of query aligns to 37:211/242 of Q5ZC82

query
sites
Q5ZC82

N

R

F

I

C

C

V

V

Y

Y

L

C

G

G

S

S

R

A

D

K

G

G

R

R

D

K

P

A

T

S

F

Y

L

Q

E

D

A

A

T

A

R

V

A

E

L

L

G

G

H

K

E

E

M

L

A

V

A

E

R

R

G

G

H

I

G

D

L

L

V

V

Y

Y

G
|
G

G

G

A

G

R

S

I

I

G

G

L

L

M

M

G

G

A

L

L

V

A

S

N

H

A

A

V

V

L

H

E

D

R

G

G

G

G

R

N

H

V

V

I

I

G

G

V

V

M

I

P

P

D

K

H

S

L

L

V

M

E

P

R

R

E

E

Q

V

A

T

H

G

E

E

G

P

L

V

P

G

T

E

L

V

I

R

R

A

V

V

N

S

N

G

M

M

H

H

E

E

R

R

K

K

A

A

S

E

M

M

A

A

A

R

H

F

A

A

D

D

A

A

F

F

I

I

A

A

L

L

P

P

G

G

I

Y

G

G

T

T

M

L

E

E

L

L

F

L

E

E

A

V

W

I

T

T

W

W

Q

A

Y

Q

L

L

G

G

L

I

H

H

D

K

K

K

P

P

I

V

G

G

V

L

L

L

D

N

I

V

A

D

G

G

F

F

Y

Y

S

D

P

P

L

F

L

L

A

S

F

F

L

I

D

D

S

M

T

A

V

V

E

S

H

E

G

G

F

F

L

I

N

A

G

E

R

D

T

A

R

R

D

R

S

I

L

I

I

I

D

S

A

A

A

P

E

T

P

A

A

R

A

E

L

L

L

V

D

L

A

K

L

L

E

E

G73 (= G39) mutation to D: In LOG-4; decreased activity.

5zblD Crystal structure of type-i log from corynebacterium glutamicum in complex with amp (see paper)
43% identity, 96% coverage: 1:178/185 of query aligns to 2:178/181 of 5zblD

query
sites
5zblD

M

L

S

Q

N

R

F

V

C

T

V

V

Y

F

L

T

G

G

S
|
S

R

A

D

L

G

G

R

S

D

S

P

S

T

L

F

Y

L

T

E

Q

A

A

T

A

R

Q

A

T

L

L

G

A

H

K

E

T

M

A

A

V

A

D

R

R

G

G

H

I

G

D

L

L

V

V

Y

Y

G

G

A

G

R

K

I

V

G

G

L

L

M
|
M

G

G

A

I

L

V

A

A

N

D

A

A

V

F

L

L

E

E

R

S

G

G

N

E

V

A

I

F

G

G

V

V

M

I

P

T

D

E

H

S

L

L

V

M

E

-

R

K

E

G

Q

L

A

G

H

H

E

E

G

K

L

L

P

T

T

E

L

L

I

E

R

I

V

V

N

P

N

D

M

M

H

H

E

I

R
|
R

K
|
K

A

R

S

R

M

M

A

A

A

E

H

L

A

G

D

D

A

G

F

F

I

I

A

A

L

M

P

P

G

G

G
|
G

I

A

G
|
G

T
|
T

M

L

E
|
E

L

L

F

F

E

E

A

V

W

W

T

T

W

W

Q

Q

Y

Q

L

L

G

G

L

I

H

H

D

Q

K

K

P

P

I

V

G

A

V

L

L

Y

D

D

I

V

A

D

G

G

F

F

Y

W

S

Q

P

P

L

L

A

E

F

M

L

L

D

E

S

Q

T

M

V

T

E

Q

H

R

G

G

F

F

L

I

N

K

G

R

R

D

T

F

R

F

D

E

S

C

L

L

I

I

D

V

A

E

A

S

E

D

P

P

A

H

A

A

L

L

D

K

A

A

L

M

E

Q

A

T

binding adenosine monophosphate: S11 (= S10), M47 (= M46), R90 (= R90), K91 (= K91), G107 (= G107), G109 (= G109), T110 (= T110), E112 (= E112), E113 (= E113)

7w2iA Crystal structure of log (rv1205) from mycobacterium tuberculosis (see paper)
43% identity, 79% coverage: 18:164/185 of query aligns to 22:168/183 of 7w2iA

query
sites
7w2iA

F

L

L

L

E

E

A

L

T

A

R

A

A

E

L

V

G

G

H

A

E

A

M

I

A

A

A

G

R

R

G

G

H

W

G

T

L

L

V

V

Y

W

G

G

A

G

R

H

I

V

G

S

L

A

M

M

G

G

A

A

L

V

A

A

N

S

A

A

V

A

L

R

E

A

R

C

G

G

N

W

V

T

I

V

G

G

V

V

M

I

P

P

D

K

H

M

L

L

V

V

E

Y

R

R

E

E

Q

L

A

A

H

D

E

H

G

D

L

A

P

D

T

E

L

L

I

I

R

V

V

T

N

D

N

T

M

M

H

W

E

E

R

R

K

K

A

Q

S

I

M

M

A

E

A

D

H

R

A

S

D

D

A

A

F

F

I

I

A

V

L

L

P

P

G

G

I

V

G

G

T

T

M

L

E

D

E

E

L

L

F

F

E
x
D

A

A

W

W

T

T

W
x
D

Q

G

Y

Y

L

L

G

G

L

T

H

H

D

D

K

K

P

P

I

I

G

V

V

M

L

V

D

D

I

P

A

W

G

G

F

H

Y

F

S

D

P

G

L

L

L

R

A

A

F

W

L

L

D

N

S

G

T

L

V

L

E

D

H

T

G

G

F

Y

L

V

N

S

G

P

R

T

T

A

R

M

D

E

S

R

L

L

I

V

binding magnesium ion: D120 (≠ E116), D124 (≠ W120)

O05306 Cytokinin riboside 5'-monophosphate phosphoribohydrolase; Protein LONELY GUY homolog; LOG homolog; EC 3.2.2.n1 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
43% identity, 79% coverage: 18:164/185 of query aligns to 26:172/187 of O05306

query
sites
O05306

F

L

L

L

E

E

A

L

T

A

R

A

A

E

L

V

G

G

H

A

E

A

M

I

A

A

A

G

R

R

G

G

H

W

G

T

L

L

V

V

Y

W

G

G

A

G

R

H

I

V

G

S

L

A

M
|
M

G

G

A

A

L

V

A

A

N

S

A

A

V

A

L

R

E

A

R

C

G

G

N

W

V

T

I

V

G

G

V

V

M

I

P

P

D
x
K

H

M

L

L

V

V

E

Y

R

R

E
|
E

Q

L

A

A

H

D

E

H

G

D

L

A

P

D

T

E

L

L

I

I

R

V

V

T

N

D

N

T

M

M

H

W

E

E

R
|
R

K

K

A

Q

S

I

M

M

A

E

A

D

H

R

A

S

D

D

A

A

F

F

I

I

A

V

L

L

P

P

G

G

I

V

G

G

T
|
T

M

L

E
x
D

E
|
E

L

L

F

F

E

D

A

A

W

W

T

T

W

D

Q

G

Y

Y

L

L

G

G

L

T

H

H

D

D

K

K

P

P

I

I

G

V

V

M

L

V

D

D

I

P

A

W

G

G

F

H

Y

F

S

D

P

G

L

L

L

R

A

A

F

W

L

L

D

N

S

G

T

L

V

L

E

D

H

T

G

G

F

Y

L

V

N

S

G

P

R

T

T

A

R

M

D

E

S

R

L

L

I

V

M54 (= M46) mutation to A: Large decrease in catalytic activity.
K74 (≠ D66) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup); mutation to A: Abrogates pupylation. This mutant accumulates in M.tuberculosis with a functional proteasome system and sensitizes M.tuberculosis to NO. Decrease in catalytic activity.
E80 (= E72) mutation to A: Large decrease in catalytic activity.
R98 (= R90) mutation to A: Loss of catalytic activity.
T118 (= T110) mutation to A: Loss of catalytic activity.
DE 120:121 (≠ EE 112:113) mutation to AA: Loss of catalytic activity.

5ajuA Crystal structure of ligand-free phosphoribohydroxylase lonely guy from claviceps purpurea in complex with phosphoribose (see paper)
34% identity, 99% coverage: 2:185/185 of query aligns to 3:209/217 of 5ajuA

query
sites
5ajuA

S

A

N

K

F

I

C

C

V

V

Y

F

L

C

G

G

S
|
S

R

S

D

G

G

G

R

A

D

S

P

P

T

A

F

H

L

M

E

E

A

A

T

A

R

R

A

Q

L

L

G

G

H

R

E

V

M

M

A

A

A

E

R

N

G

N

H

I

G

D

L

L

V

V

Y

Y

G

G

A

G

R

T

I

V

G

G

L

L

M

M

G

G

A

E

L

V

A

A

N

R

A

T

V

V

L

C

E

S

R

I

G

N

G

G

-

P

-

E

N

S

V

V

I

H

G

G

V

I

M

I

P

P

D

E

H

A

L

L

V

V

-

R

-

Y

E

E

R

R

E

D

Q

G

A

T

H

Y

E

Q

G

T

L

V

-

K

-

D

-

N

-

K

-

Q

-

V

P

P

T

T

-

E

-

T

-

V

-

Y

-

G

-

R

L

T

I

T

R

V

V

V

N

K

N

D

M

M

H

H

E

T

R
|
R

K

K

A

K

S

M

M

M

A

A

A

E

H

E

A

V

-

I

-

S

-

G

-

P

-

G

D

S

A

G

F

F

I

I

A

G

L

L

P

S

G

G

G
|
G

I
x
Y

G
|
G

T
|
T

M

M

E

E

E
|
E

L

V

F

F

E

E

A

V

W

I

T

T

W

W

Q

N

Y

Q

L

L

G

G

L

I

H

H

D

T

K

K

P

G

I

I

G

C

V

L

L

L

D

N

I

V

A

E

G

G

F

Y

Y

W

S

D

P

G

L

I

L

L

A

Q

F

W

L

I

D

N

S

M

T

A

V

A

E

A

H

Q

G

G

F

F

L

V

N

Q

G

P

R

G

T

N

R

E

D

T

S

I

L

V

I

V

D

S

A

A

A

G

E

D

P

A

A

E

A

G

L

A

L

V

D

R

A

A

L

L

E

R

A

E

R

Y

L

K

A

V

S

S

P

E

V

A

T

T

binding 5-O-phosphono-beta-D-ribofuranose: S11 (= S10), R108 (= R90), G131 (= G107), Y132 (≠ I108), G133 (= G109), T134 (= T110), E137 (= E113)

3sbxA Crystal structure of a putative uncharacterized protein from mycobacterium marinum bound to adenosine 5'-monophosphate amp (see paper)
43% identity, 81% coverage: 16:164/185 of query aligns to 15:163/177 of 3sbxA

query
sites
3sbxA

P

P

T

E

F

L

L

L

E

E

A

L

T

A

R

G

A

A

L

V

G

G

H

A

E

A

M

I

A

A

R

R

G

G

H

W

G

T

L

L

V

V

Y

W

G

G

A

G

R

H

I

V

G

S

L

A

M

M

G

G

A

A

L

V

A

S

N

S

A

A

V

A

L

R

E

A

R

H

G

G

N

W

V

T

I

V

G

G

V

V

M

I

P

P

D

K

H

M

L

L

V

V

E

H

R

R

E
|
E

Q

L

A

A

H

D

E

H

G

D

L

A

P

D

T

E

L

L

I

V

R

V

V

T

N

E

N

T

M
|
M

H

W

E

E

R
|
R

K
|
K

A

Q

S

V

M

M

A

E

A

D

H

R

A

A

D

N

A

A

F

F

I

I

A

T

L

L

P

P

G

G

G
|
G

I

V

G
|
G

T
|
T

M

L

E
x
D

E
|
E

L

L

F

L

E

D

A

V

W

W

T

T

W

E

Q

G

Y

Y

L

L

G

G

L

M

H

H

D

D

K

K

P

S

I

I

G

V

V

V

L

L

D

D

I

P

A

W

G

G

F

H

Y

F

S

D

P

G

L

L

L

R

A

A

F

W

L

L

D

S

S

E

T

L

V

A

E

D

H

T

G

G

F

Y

L

V

N

S

G

R

R

T

T

A

R

M

D

E

S

R

L

L

I

I

binding adenosine monophosphate: E71 (= E72), M86 (= M87), R89 (= R90), K90 (= K91), G106 (= G107), G108 (= G109), T109 (= T110), D111 (≠ E112), E112 (= E113)

Query Sequence

>WP_086508957.1 NCBI__GCF_002151265.1:WP_086508957.1
MSNFCVYLGSRDGRDPTFLEATRALGHEMAARGHGLVYGGARIGLMGALANAVLERGGNV
IGVMPDHLVEREQAHEGLPTLIRVNNMHERKASMAAHADAFIALPGGIGTMEELFEAWTW
QYLGLHDKPIGVLDIAGFYSPLLAFLDSTVEHGFLNGRTRDSLIDAAEPAALLDALEARL
ASPVT

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory