SitesBLAST
Comparing WP_086508980.1 NCBI__GCF_002151265.1:WP_086508980.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
Q5F8J4 Homoserine dehydrogenase; HDH; HSD; NAD(+)-dependent homoserine dehydrogenase; NAD(+)-dependent HSD; NgHSD; EC 1.1.1.3 from Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) (see paper)
54% identity, 99% coverage: 1:434/438 of query aligns to 1:433/435 of Q5F8J4
- L45 (= L53) mutation to R: Shows a marked increase in the catalytic efficiency with NADP(+).
- LS 45:46 (≠ LT 53:54) mutation to RD: Does not impair the catalytic activity with NAD(+). Slightly increases the activity, but slightly decreases the affinity for NADP(+).; mutation to RR: Causes a shift in coenzyme preference from NAD(+) to NADP(+) by a factor of 974. Shows a slight decrease in the catalytic efficiency with NAD(+) and a 4.5-fold increase in catalytic efficiency with NADP(+).
6dzsA Mycobacterial homoserine dehydrogenase thra in complex with NADP
39% identity, 98% coverage: 2:432/438 of query aligns to 3:429/431 of 6dzsA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G10), L12 (= L11), G13 (= G12), N14 (≠ T13), V15 (= V14), V45 (≠ L44), R46 (= R45), R47 (≠ S46), R52 (≠ C51), I63 (≠ V62), L78 (≠ V77), M79 (≠ L78), P84 (≠ V83), A102 (= A101), K104 (= K103), G306 (= G300), T310 (= T304)
4pg7A Crystal structure of s. Aureus homoserine dehydrogenase at ph7.5 (see paper)
42% identity, 72% coverage: 1:316/438 of query aligns to 2:307/402 of 4pg7A
Sites not aligning to the query:
6a0tB Homoserine dehydrogenase k99a mutant from thermus thermophilus hb8 complexed with hse and NADP+ (see paper)
35% identity, 71% coverage: 1:313/438 of query aligns to 1:303/332 of 6a0tB
- active site: D191 (= D199), K195 (= K203)
- binding l-homoserine: N150 (= N157), G151 (= G158), T152 (= T159), Y178 (= Y186), E180 (= E188), D186 (= D194), K195 (= K203)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G10 (= G10), G11 (≠ L11), G12 (= G12), T13 (= T13), V14 (= V14), L42 (vs. gap), V43 (vs. gap), R44 (= R31), D45 (≠ R32), K48 (≠ R35), R50 (≠ I37), A73 (≠ V77), M74 (≠ L78), G75 (= G79), A97 (= A101), N98 (= N102), G177 (= G185), E180 (= E188), A289 (= A299), G290 (= G300), T294 (= T304)
6a0sA Homoserine dehydrogenase from thermus thermophilus hb8 complexed with hse and NADPH (see paper)
35% identity, 71% coverage: 1:313/438 of query aligns to 1:303/331 of 6a0sA
- active site: D191 (= D199), K195 (= K203)
- binding l-homoserine: K99 (= K103), N150 (= N157), G151 (= G158), T152 (= T159), Y178 (= Y186), E180 (= E188), D186 (= D194), K195 (= K203)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G11 (≠ L11), G12 (= G12), T13 (= T13), V14 (= V14), L42 (vs. gap), V43 (vs. gap), R44 (= R31), D45 (≠ R32), K48 (≠ R35), R50 (≠ I37), A73 (≠ V77), M74 (≠ L78), A97 (= A101), K99 (= K103), G177 (= G185), E180 (= E188), A289 (= A299), G290 (= G300), T294 (= T304)
2ejwA Homoserine dehydrogenase from thermus thermophilus hb8
35% identity, 71% coverage: 1:313/438 of query aligns to 1:303/331 of 2ejwA
F9VNG5 Homoserine dehydrogenase; HDH; HSD; StHSD; EC 1.1.1.3 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see 3 papers)
36% identity, 59% coverage: 61:318/438 of query aligns to 56:302/304 of F9VNG5
- S73 (vs. gap) binding NADP(+)
- T100 (≠ A101) binding NADP(+)
- K102 (= K103) binding NADP(+)
- G182 (= G185) binding NADP(+)
- E185 (= E188) binding NADP(+)
- G284 (= G300) binding NADP(+)
Sites not aligning to the query:
- 8 binding NADP(+)
- 10 binding NADP(+)
- 11 binding NADP(+)
- 38 binding NADP(+)
- 39 binding NADP(+)
- 303 modified: Interchain
7f4cA The crystal structure of the immature holo-enzyme of homoserine dehydrogenase complexed with NADP and 1,4-butandiol from the hyperthermophilic archaeon sulfurisphaera tokodaii. (see paper)
37% identity, 59% coverage: 61:318/438 of query aligns to 56:298/300 of 7f4cA
Sites not aligning to the query:
5x9dA Crystal structure of homoserine dehydrogenase in complex with l- cysteine and NAD (see paper)
36% identity, 59% coverage: 61:318/438 of query aligns to 56:300/302 of 5x9dA
- active site: D196 (= D199), K200 (= K203)
- binding (2R)-3-[[(4S)-3-aminocarbonyl-1-[(2R,3R,4S,5R)-5-[[[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxymethyl]-3,4-bis(oxidanyl)oxolan-2-yl]-4H-pyridin-4-yl]sulfanyl]-2-azanyl-propanoic acid: V72 (= V77), S73 (vs. gap), S74 (vs. gap), P82 (≠ V83), T100 (≠ A101), N101 (= N102), K102 (= K103), G127 (≠ A128), S131 (≠ G132), N155 (= N157), G156 (= G158), T157 (= T159), Y183 (= Y186), A184 (= A187), E185 (= E188), D191 (= D194), D196 (= D199), K200 (= K203), A281 (= A299), G282 (= G300), A286 (≠ T304)
Sites not aligning to the query:
- binding (2R)-3-[[(4S)-3-aminocarbonyl-1-[(2R,3R,4S,5R)-5-[[[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxymethyl]-3,4-bis(oxidanyl)oxolan-2-yl]-4H-pyridin-4-yl]sulfanyl]-2-azanyl-propanoic acid: 6, 7, 8, 9, 10, 11, 37, 38
4xb1A Hyperthermophilic archaeal homoserine dehydrogenase in complex with NADPH (see paper)
33% identity, 55% coverage: 72:313/438 of query aligns to 86:309/319 of 4xb1A
Sites not aligning to the query:
O58802 Homoserine dehydrogenase; HDH; HSD; HseDH; EC 1.1.1.3 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see paper)
33% identity, 55% coverage: 72:313/438 of query aligns to 86:309/319 of O58802
- S92 (≠ G79) binding NADPH
- S93 (≠ G80) binding NADPH
- S114 (≠ A101) binding NADPH
- K116 (= K103) binding NADPH
- E140 (= E127) binding Na(+)
- V143 (= V130) binding Na(+)
- A145 (≠ G132) binding Na(+)
- T147 (≠ I134) binding Na(+)
- G296 (= G300) binding NADPH
Sites not aligning to the query:
- 10 binding NADPH
- 12 binding NADPH
- 13 binding NADPH
- 40 binding NADPH; R→A: Increases activity with NADP.
- 57 binding NADPH; K→A: Increases activity with NADP.
4xb2A Hyperthermophilic archaeal homoserine dehydrogenase mutant in complex with NADPH (see paper)
33% identity, 55% coverage: 72:313/438 of query aligns to 86:309/319 of 4xb2A
- active site: D211 (= D199), K215 (= K203)
- binding l-homoserine: A171 (≠ G158), S172 (≠ T159), D206 (= D194), K215 (= K203)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: V91 (= V77), S92 (≠ G79), S93 (≠ G80), S114 (≠ A101), N115 (= N102), K116 (= K103), S141 (≠ A128), G295 (≠ A299), T300 (= T304)
Sites not aligning to the query:
3jsaA Homoserine dehydrogenase from thermoplasma volcanium complexed with NAD
30% identity, 54% coverage: 1:236/438 of query aligns to 1:249/321 of 3jsaA
- active site: D212 (= D199), K216 (= K203)
- binding nicotinamide-adenine-dinucleotide: G10 (= G10), L11 (= L11), G12 (= G12), N13 (≠ T13), V14 (= V14), D42 (= D27), S43 (≠ E28), A90 (≠ V77), T91 (≠ L78), P92 (vs. gap), A117 (= A101), N118 (= N102), A144 (= A128)
Sites not aligning to the query:
3ingA Crystal structure of homoserine dehydrogenase (np_394635.1) from thermoplasma acidophilum at 1.95 a resolution
31% identity, 34% coverage: 86:232/438 of query aligns to 97:242/319 of 3ingA
Sites not aligning to the query:
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 9, 10, 11, 12, 13, 38, 39, 57, 85, 86, 87, 302, 305
O81852 Bifunctional aspartokinase/homoserine dehydrogenase 2, chloroplastic; AK-HD 2; AK-HSDH 2; Beta-aspartyl phosphate homoserine 2; EC 2.7.2.4; EC 1.1.1.3 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
26% identity, 56% coverage: 70:316/438 of query aligns to 626:909/916 of O81852
Sites not aligning to the query:
- 441 I→A: Loss of threonine sensitivity for the aspartokinase activity and decreased inhibition of homoserine dehydrogenase activity by threonine.
- 443 Q→A: Loss of threonine sensitivity for the aspartokinase activity and decreased inhibition of homoserine dehydrogenase activity by threonine.
- 522 I→A: No effect on the inhibition of aspartokinase activity by threonine, but decreased inhibition of homoserine dehydrogenase activity by threonine.
- 524 Q→A: No effect on the inhibition of aspartokinase activity by threonine, but decreased inhibition of homoserine dehydrogenase activity by threonine.
O94671 Homoserine dehydrogenase; HDH; HSD; EC 1.1.1.3 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
32% identity, 32% coverage: 71:211/438 of query aligns to 79:239/376 of O94671