SitesBLAST
Comparing WP_086509083.1 NCBI__GCF_002151265.1:WP_086509083.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 11 hits to proteins with known functional sites (download)
8w78A Structure of drosophila melanogaster l-2-hydroxyglutarate dehydrogenase in complex with fad and 2-oxoglutarate (see paper)
41% identity, 95% coverage: 15:392/399 of query aligns to 16:403/410 of 8w78A
- binding 2-oxoglutaric acid: S48 (= S47), H52 (= H51), Y245 (= Y241), F254 (= F250), L255 (= L251), H258 (= H254), R349 (= R345)
- binding flavin-adenine dinucleotide: E34 (= E33), K35 (= K34), H42 (= H41), Q43 (= Q42), S44 (≠ T43), N47 (= N46), S48 (= S47), V50 (= V49), H52 (= H51), V173 (= V171), G203 (≠ S199), G204 (= G200), Q206 (≠ M202), R349 (= R345), P387 (= P376), G388 (≠ A377), A389 (= A378), T390 (= T379)
Sites not aligning to the query:
8w7fB Structure of drosophila melanogaster l-2-hydroxyglutarate dehydrogenase bound with fad and a sulfate ion (see paper)
41% identity, 95% coverage: 15:392/399 of query aligns to 16:405/412 of 8w7fB
- binding flavin-adenine dinucleotide: E34 (= E33), K35 (= K34), H42 (= H41), Q43 (= Q42), S44 (≠ T43), H46 (= H45), N47 (= N46), S48 (= S47), V50 (= V49), H52 (= H51), V173 (= V171), G205 (≠ S199), G206 (= G200), Q208 (≠ M202), Y231 (= Y225), V350 (= V344), R351 (= R345), P389 (= P376), G390 (≠ A377), A391 (= A378), T392 (= T379)
Sites not aligning to the query:
3dmeA Crystal structure of conserved exported protein from bordetella pertussis. Northeast structural genomics target ber141
31% identity, 67% coverage: 3:268/399 of query aligns to 4:271/366 of 3dmeA
- binding flavin-adenine dinucleotide: I8 (≠ L7), G9 (= G8), G11 (= G10), V12 (≠ I11), V13 (≠ L12), E32 (= E33), A33 (≠ K34), T41 (≠ Q42), S42 (≠ T43), R44 (≠ H45), N45 (= N46), S46 (= S47), V48 (= V49), H50 (= H51), P170 (≠ T172), L171 (≠ G173), A203 (≠ S199)
Sites not aligning to the query:
4x9mA Oxidized l-alpha-glycerophosphate oxidase from mycoplasma pneumoniae with fad bound (see paper)
27% identity, 69% coverage: 3:279/399 of query aligns to 5:286/384 of 4x9mA
- binding flavin-adenine dinucleotide: G10 (= G8), G12 (= G10), I14 (≠ L12), E33 (= E33), K34 (= K34), E41 (≠ H41), T42 (≠ Q42), S43 (≠ T43), A45 (≠ H45), N46 (= N46), S47 (= S47), V49 (= V49), H51 (= H51), E176 (≠ H170), V177 (= V171), A209 (≠ S199), G210 (= G200), Y212 (≠ M202), Y234 (= Y225)
- binding sn-glycerol-3-phosphate: S47 (= S47), H51 (= H51), K258 (≠ L251), G259 (= G252)
Sites not aligning to the query:
P75063 Glycerol 3-phosphate oxidase; GlpO; L-alpha-glycerophosphate oxidase; EC 1.1.3.21 from Mycoplasma pneumoniae (strain ATCC 29342 / M129 / Subtype 1) (Mycoplasmoides pneumoniae) (see paper)
27% identity, 69% coverage: 3:279/399 of query aligns to 5:286/384 of P75063
- I14 (≠ L12) binding FAD
- E33 (= E33) binding FAD
- TS 42:43 (≠ QT 42:43) binding FAD
- SGV 47:49 (= SGV 47:49) binding FAD
- V177 (= V171) binding FAD
Sites not aligning to the query:
- 346:347 binding FAD
- 352 binding FAD
Q9AGP8 Dimethylglycine oxidase; DMGO; EC 1.5.3.10 from Arthrobacter globiformis (see 2 papers)
25% identity, 52% coverage: 4:211/399 of query aligns to 7:215/830 of Q9AGP8
Sites not aligning to the query:
- 225 Important for catalytic activity; H→Q: Reduces catalytic efficiency 3-fold and substrate affinity 30-fold.
- 259 Important for catalytic activity; binding FAD; Y→F: Reduces catalytic efficiency 225-fold and substrate affinity 25-fold.
- 360:363 binding FAD
- 539 binding (6S)-5,6,7,8-tetrahydrofolate
- 552 Important for catalytic activity; D→A: No effect on the activity.; D→N: Reduces activity 3-fold.
1pj7A Structure of dimethylglycine oxidase of arthrobacter globiformis in complex with folinic acid (see paper)
25% identity, 52% coverage: 4:211/399 of query aligns to 4:212/827 of 1pj7A
- binding flavin-adenine dinucleotide: G8 (= G8), G10 (= G10), I11 (= I11), V12 (≠ L12), D32 (≠ E35), Q33 (≠ S36), G41 (= G44), S42 (≠ H45), T43 (≠ N46), H45 (≠ G48), P47 (≠ I50), L49 (≠ A52), T170 (≠ H170), V171 (= V171), A200 (≠ S199), G201 (= G200), W203 (≠ M202)
Sites not aligning to the query:
- active site: 222, 256, 549
- binding flavin-adenine dinucleotide: 222, 256, 331, 357, 358, 359, 360
- binding N-[4-({[(6S)-2-amino-5-formyl-4-oxo-3,4,5,6,7,8-hexahydropteridin-6-yl]methyl}amino)benzoyl]-L-glutamic acid: 505, 536, 549, 551, 563, 629, 648, 655, 696
3gsiA Crystal structure of d552a dimethylglycine oxidase mutant of arthrobacter globiformis in complex with tetrahydrofolate (see paper)
25% identity, 52% coverage: 4:211/399 of query aligns to 4:212/827 of 3gsiA
- binding flavin-adenine dinucleotide: G10 (= G10), I11 (= I11), V12 (≠ L12), D32 (≠ E35), Q33 (≠ S36), G41 (= G44), S42 (≠ H45), T43 (≠ N46), H45 (≠ G48), P47 (≠ I50), L49 (≠ A52), T170 (≠ H170), V171 (= V171), A200 (≠ S199), G201 (= G200), W203 (≠ M202)
Sites not aligning to the query:
- active site: 222, 256, 549
- binding flavin-adenine dinucleotide: 222, 256, 330, 331, 332, 357, 358, 359, 360
- binding magnesium ion: 254, 409
- binding (6s)-5,6,7,8-tetrahydrofolate: 505, 536, 551, 563, 629, 648, 655, 696
1pj6A Crystal structure of dimethylglycine oxidase of arthrobacter globiformis in complex with folic acid (see paper)
25% identity, 52% coverage: 4:211/399 of query aligns to 5:213/828 of 1pj6A
- binding flavin-adenine dinucleotide: G9 (= G8), G11 (= G10), I12 (= I11), V13 (≠ L12), D33 (≠ E35), Q34 (≠ S36), G42 (= G44), S43 (≠ H45), T44 (≠ N46), H46 (≠ G48), P48 (≠ I50), L50 (≠ A52), V172 (= V171), A201 (≠ S199), G202 (= G200), W204 (≠ M202)
Sites not aligning to the query:
7cyxA Crystal strcuture of glycine oxidase from bacillus cereus atcc 14579 (see paper)
26% identity, 56% coverage: 2:224/399 of query aligns to 2:223/363 of 7cyxA
- binding flavin-adenine dinucleotide: I7 (≠ L7), G8 (= G8), G10 (= G10), V11 (≠ I11), I12 (≠ L12), V30 (≠ L32), E31 (= E33), K32 (= K34), E38 (≠ H41), A39 (≠ Q42), S40 (≠ T43), A43 (≠ N46), G45 (= G48), L46 (≠ V49), V171 (= V171), G200 (≠ S199), G201 (= G200), W203 (≠ M202)
Sites not aligning to the query:
Q8GAI3 4-methylaminobutanoate oxidase (formaldehyde-forming); MABO; Demethylating gamma-N-methylaminobutyrate oxidase; Gamma-N-methylaminobutyrate oxidase 1; EC 1.5.3.19 from Paenarthrobacter nicotinovorans (Arthrobacter nicotinovorans) (see paper)
27% identity, 49% coverage: 5:201/399 of query aligns to 29:226/824 of Q8GAI3
- W66 (≠ Q40) mutation W->F,S: Contains a non-covalently bound FAD. Loss of enzyme activity.
- H67 (= H41) mutation to A: Contains a non-covalently bound FAD. Exhibits about 10% of the wild-type enzyme activity.
Query Sequence
>WP_086509083.1 NCBI__GCF_002151265.1:WP_086509083.1
MYDFIILGGGILGLSTAMQLIERYPEKKMLLLEKESGPAQHQTGHNSGVIHAGVYYAPGS
LKARFCLEGNRATKEFCDRHAIPYEVCGKLLVATNEQEMQRMEALWERTAANGLEREWLS
AGELKEREPHITGQGAIFVPSSGIVSYARVAEAMADEFQRHGGQIRYDHHVTGLAERTGE
VIVGTSQGEFNSRYLVTCSGLMADRVVRLLGRDPGFTICPFRGEYYRLPDRHSDIVKHLI
YPIPDPSMPFLGVHLTRMIDGSVTVGPNAVLALKREGYRKSDVSLVDMARMFSNPGILKV
LGRNLRPGIHELKNSLWRRGYLEEVRKYCPSLTLDDLEPWPAGVRAQAVSRDGRLIDDFL
FVNTRRTVNVCNAPSPAATSALPIGRHILDKVSAMVQSS
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory