SitesBLAST
Comparing WP_086509188.1 NCBI__GCF_002151265.1:WP_086509188.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3wx9A Crystal structure of pyrococcus horikoshii kynurenine aminotransferase in complex with pmp, gla, 4ad, 2og, glu and kya
29% identity, 80% coverage: 89:471/476 of query aligns to 20:403/404 of 3wx9A
- binding 4-(2-aminophenyl)-2,4-dioxobutanoic acid: R23 (≠ D92), G40 (= G113), L41 (≠ I114)
- binding 2-oxoglutaric acid: D213 (= D282), P214 (≠ I283), Y215 (= Y284), G216 (= G285), E217 (≠ D286), G241 (≠ S310), T242 (≠ A311), I246 (≠ T315)
- binding (2E)-pent-2-enedioic acid: G40 (= G113), Y130 (≠ F203), N184 (= N253), R376 (= R444)
- binding glutamic acid: S20 (= S89), V22 (≠ W91), L131 (≠ Y204), V360 (≠ T428), A364 (≠ L432), R369 (≠ G437)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G104 (= G177), S105 (≠ C178), Q106 (= Q179), Y130 (≠ F203), N184 (= N253), D212 (= D281), P214 (≠ I283), Y215 (= Y284), T242 (≠ A311), S244 (= S313), K245 (= K314), R252 (= R321)
3av7A Crystal structure of pyrococcus horikoshii kynurenine aminotransferase in complex with pmp, kyn as substrates and kya as products
29% identity, 80% coverage: 89:471/476 of query aligns to 20:403/404 of 3av7A
- binding 4-hydroxyquinoline-2-carboxylic acid: S20 (= S89), V22 (≠ W91), R23 (≠ D92), L131 (≠ Y204), Q135 (= Q208), A364 (≠ L432), R369 (≠ G437)
- binding (2S)-2-amino-4-(2-aminophenyl)-4-oxobutanoic acid: R23 (≠ D92), G40 (= G113), Y130 (≠ F203), L131 (≠ Y204), A132 (≠ G205), N184 (= N253), R376 (= R444)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G104 (= G177), S105 (≠ C178), Q106 (= Q179), Y130 (≠ F203), V179 (≠ T248), N184 (= N253), D212 (= D281), P214 (≠ I283), Y215 (= Y284), T242 (≠ A311), S244 (= S313), K245 (= K314), R252 (= R321)
3aowC Crystal structure of pyrococcus horikoshii kynurenine aminotransferase in complex with akg
29% identity, 80% coverage: 89:471/476 of query aligns to 20:403/404 of 3aowC
- binding 2-oxoglutaric acid: R23 (≠ D92), Y70 (= Y143), Y130 (≠ F203), L275 (≠ G343)
- binding pyridoxal-5'-phosphate: G104 (= G177), S105 (≠ C178), Q106 (= Q179), Y130 (≠ F203), V179 (≠ T248), N184 (= N253), D212 (= D281), P214 (≠ I283), Y215 (= Y284), T242 (≠ A311), S244 (= S313), K245 (= K314), R252 (= R321)
3aovA Crystal structure of pyrococcus horikoshii kynurenine aminotransferase in complex with plp
29% identity, 80% coverage: 89:471/476 of query aligns to 20:403/404 of 3aovA
- binding pyridoxal-5'-phosphate: G104 (= G177), S105 (≠ C178), Q106 (= Q179), Y130 (≠ F203), V179 (≠ T248), N184 (= N253), D212 (= D281), P214 (≠ I283), Y215 (= Y284), T242 (≠ A311), S244 (= S313), K245 (= K314), R252 (= R321)
1wstA Crystal structure of multiple substrate aminotransferase (msat) from thermococcus profundus
27% identity, 80% coverage: 86:466/476 of query aligns to 14:396/403 of 1wstA
- binding pyridoxal-5'-phosphate: G102 (= G177), S103 (≠ C178), Q104 (= Q179), Y128 (≠ F203), V177 (≠ T248), N182 (= N253), D210 (= D281), P212 (≠ I283), Y213 (= Y284), T240 (≠ A311), S242 (= S313), K243 (= K314), R250 (= R321)
2zc0A Crystal structure of an archaeal alanine:glyoxylate aminotransferase (see paper)
28% identity, 75% coverage: 106:464/476 of query aligns to 35:399/405 of 2zc0A
- active site: Y132 (≠ F203), D214 (= D281), A216 (≠ I283), S246 (= S313)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G106 (= G177), G107 (≠ C178), T108 (≠ Q179), Y132 (≠ F203), N186 (= N253), D214 (= D281), A216 (≠ I283), Y217 (= Y284), T244 (≠ A311), S246 (= S313), K247 (= K314), R254 (= R321)
8tn3A Structure of s. Hygroscopicus aminotransferase mppq complexed with pyridoxamine 5'-phosphate (pmp) (see paper)
34% identity, 62% coverage: 169:465/476 of query aligns to 84:386/388 of 8tn3A
1vp4A Crystal structure of a putative aminotransferase (tm1131) from thermotoga maritima msb8 at 1.82 a resolution
25% identity, 78% coverage: 96:466/476 of query aligns to 30:410/420 of 1vp4A
- binding pyridoxal-5'-phosphate: G112 (= G177), S113 (≠ C178), Q114 (= Q179), Y138 (≠ F203), N194 (= N253), D222 (= D281), P224 (≠ I283), Y225 (= Y284), T252 (≠ A311), S254 (= S313), K255 (= K314), R262 (= R321)
2zyjA Crystal structure of lysn, alpha-aminoadipate aminotransferase (complexed with n-(5'-phosphopyridoxyl)-l-glutamate), from thermus thermophilus hb27 (see paper)
31% identity, 69% coverage: 141:470/476 of query aligns to 68:394/397 of 2zyjA
- binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-glutamic acid: G99 (= G177), S100 (≠ C178), Q101 (= Q179), Y125 (≠ F203), N174 (= N253), D202 (= D281), Y205 (= Y284), S235 (≠ A311), S237 (= S313), K238 (= K314), R245 (= R321), R368 (= R444)
Sites not aligning to the query:
Q72LL6 2-aminoadipate transaminase; 2-aminoadipate aminotransferase; Alpha-aminoadipate aminotransferase; AAA-AT; AadAT; EC 2.6.1.39 from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) (see 2 papers)
31% identity, 69% coverage: 141:470/476 of query aligns to 68:394/397 of Q72LL6
- Y70 (= Y143) binding
- N174 (= N253) binding ; binding
- R245 (= R321) binding
- R368 (= R444) binding
Sites not aligning to the query:
- 20 S→E: Strongly decreases the affinity for AAA and Glu. A mild decrease of affinity is observed for 2-oxoglutarate. Increases the affinity for leucine and 2-oxoisocaproate.
- 23 R→A: Strongly decreases the affinity for AAA and Glu. A mild decrease of affinity is observed for 2-oxoglutarate which has the same chain length as Glu, but differs by the presence of a 2-oxo group which is not recognized by R-23. Increases the affinity for leucine and 2-oxoisocaproate due to the absence of gamma-carboxyl group.; R→Q: Strongly decreases the affinity for AAA and Glu. A mild decrease of affinity is observed for 2-oxoglutarate which has the same chain length as Glu, but differs by the presence of a 2-oxo group which is not recognized by R-23. Increases the affinity for leucine and 2-oxoisocaproate due to the absence of gamma-carboxyl group.
- 40 binding
3cbfA Crystal structure of lysn, alpha-aminoadipate aminotransferase, from thermus thermophilus hb27 (see paper)
31% identity, 69% coverage: 141:470/476 of query aligns to 64:390/392 of 3cbfA
- binding (2S)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]hexanedioic acid: G95 (= G177), S96 (≠ C178), Q97 (= Q179), Y121 (≠ F203), N170 (= N253), D198 (= D281), Y201 (= Y284), S231 (≠ A311), S233 (= S313), K234 (= K314), R241 (= R321), R364 (= R444)
Sites not aligning to the query:
2egyA Crystal structure of lysn, alpha-aminoadipate aminotransferase (substrate free form), from thermus thermophilus hb27
31% identity, 69% coverage: 141:470/476 of query aligns to 64:390/392 of 2egyA
- binding pyridoxal-5'-phosphate: G95 (= G177), S96 (≠ C178), Q97 (= Q179), Y121 (≠ F203), N170 (= N253), D198 (= D281), A200 (≠ I283), Y201 (= Y284), S231 (≠ A311), S233 (= S313), K234 (= K314), R241 (= R321)
2z1yA Crystal structure of lysn, alpha-aminoadipate aminotransferase (complexed with n-(5'-phosphopyridoxyl)-l-leucine), from thermus thermophilus hb27
31% identity, 69% coverage: 141:470/476 of query aligns to 60:386/389 of 2z1yA
- binding leucine: Y117 (≠ F203), R360 (= R444)
- binding pyridoxal-5'-phosphate: G91 (= G177), S92 (≠ C178), Q93 (= Q179), Y117 (≠ F203), N166 (= N253), D194 (= D281), Y197 (= Y284), S227 (≠ A311), S229 (= S313), K230 (= K314), R237 (= R321)
Sites not aligning to the query:
4gebA Kynurenine aminotransferase ii inhibitors (see paper)
29% identity, 55% coverage: 171:433/476 of query aligns to 110:387/428 of 4gebA
- binding (5-hydroxy-4-{[(7-hydroxy-6-oxo-2-phenyl-6,7-dihydro-2H-pyrazolo[3,4-b]pyridin-5-yl)amino]methyl}-6-methylpyridin-3-yl)methyl dihydrogen phosphate: S117 (≠ C178), Q118 (= Q179), Y142 (≠ F203), N202 (= N253), D230 (= D281), P232 (≠ I283), Y233 (= Y284), S260 (≠ A311), S262 (= S313), K263 (= K314), R270 (= R321)
Sites not aligning to the query:
4ge9A Kynurenine aminotransferase ii inhibitors (see paper)
29% identity, 55% coverage: 171:433/476 of query aligns to 110:387/428 of 4ge9A
- binding (4-{[(6-benzyl-1-hydroxy-7-methoxy-2-oxo-1,2-dihydroquinolin-3-yl)amino]methyl}-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate: S117 (≠ C178), Q118 (= Q179), Y142 (≠ F203), N202 (= N253), D230 (= D281), P232 (≠ I283), Y233 (= Y284), S260 (≠ A311), S262 (= S313), R270 (= R321), L293 (≠ G343)
Sites not aligning to the query:
4ge7A Kynurenine aminotransferase ii inhibitors (see paper)
29% identity, 55% coverage: 171:433/476 of query aligns to 110:387/428 of 4ge7A
- binding (5-hydroxy-4-{[(1-hydroxy-2-oxo-6-phenoxy-1,2-dihydroquinolin-3-yl)amino]methyl}-6-methylpyridin-3-yl)methyl dihydrogen phosphate: S117 (≠ C178), Q118 (= Q179), Y142 (≠ F203), N202 (= N253), D230 (= D281), P232 (≠ I283), Y233 (= Y284), S260 (≠ A311), S262 (= S313), R270 (= R321), L293 (≠ G343)
Sites not aligning to the query:
4gdyB Kynurenine aminotransferase ii inhibitors
29% identity, 55% coverage: 171:433/476 of query aligns to 110:387/428 of 4gdyB
- binding (5-hydroxy-6-methyl-4-{[(1-oxo-7-phenoxy-1,2-dihydro[1,2,4]triazolo[4,3-a]quinolin-4-yl)amino]methyl}pyridin-3-yl)methyl dihydrogen phosphate: G116 (= G177), S117 (≠ C178), Q118 (= Q179), Y142 (≠ F203), N202 (= N253), D230 (= D281), P232 (≠ I283), S260 (≠ A311), S262 (= S313), R270 (= R321)
Sites not aligning to the query:
4ge4A Kynurenine aminotransferase ii inhibitors (see paper)
29% identity, 55% coverage: 171:433/476 of query aligns to 104:381/422 of 4ge4A
- binding (5-hydroxy-4-{[(1-hydroxy-7-methoxy-2-oxo-1,2-dihydroquinolin-3-yl)amino]methyl}-6-methylpyridin-3-yl)methyl dihydrogen phosphate: G110 (= G177), S111 (≠ C178), Q112 (= Q179), Y136 (≠ F203), N196 (= N253), D224 (= D281), P226 (≠ I283), Y227 (= Y284), S254 (≠ A311), S256 (= S313), K257 (= K314), R264 (= R321)
Sites not aligning to the query:
5tf5A Crystal structure of human kat-2 in complex with a reversible inhibitor
29% identity, 55% coverage: 171:433/476 of query aligns to 110:387/425 of 5tf5A
Sites not aligning to the query:
2r2nA The crystal structure of human kynurenine aminotransferase ii in complex with kynurenine (see paper)
29% identity, 55% coverage: 171:433/476 of query aligns to 110:387/425 of 2r2nA
- binding (2S)-2-amino-4-(2-aminophenyl)-4-oxobutanoic acid: Y142 (≠ F203)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: S117 (≠ C178), Q118 (= Q179), Y142 (≠ F203), N202 (= N253), D230 (= D281), P232 (≠ I283), Y233 (= Y284), S260 (≠ A311), S262 (= S313), K263 (= K314), R270 (= R321)
Sites not aligning to the query:
Query Sequence
>WP_086509188.1 NCBI__GCF_002151265.1:WP_086509188.1
MKRYDQVAQTLTRRIEQGQYRVGDRLPSVRMLCGELGVSVSTVQEAYRRLEEAGLAEARP
RSGYYVLPRPTPSLLPKVSRPAQRPLDVSQWDQVLELVTRSPDEDRLLMLGRGIPDLAAA
SLRPLQRQLGTLHRHVAAHGLNYDSLQGSLALRRQVVRLAAKSGCLLHPDDVLITTGCQE
ALSLALRTLTEPGDVVAVDSPSFYGTMQILKAQKLKALEIPTDPRTGISLEALELALEQW
PIKAIQITPTCNNPLGYTMPEARKRALVALAQRFDVAIVEDDIYGDLSFEAPRPRTLKSF
DDDGRVLLCSAFSKTLVPGLRVGWMAPGRYRDQALHMKYVSTGASATLPQLAVAEFVEHG
HYERHLREMLRQYRRHRDIMIDWVTRYFPAGTGVSFPQGGFLLWLELPGDIDCVRLKEQL
ANEGLHITPGSLFSASGKFRHCLRLNYAAAMTPTVEAGIKRVGEAVAALLEQEVSA
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory