SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_086509530.1 NCBI__GCF_002151265.1:WP_086509530.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P07821 Iron(3+)-hydroxamate import ATP-binding protein FhuC; Ferric hydroxamate uptake protein C; Ferrichrome transport ATP-binding protein FhuC; Iron(III)-hydroxamate import ATP-binding protein FhuC; EC 7.2.2.16 from Escherichia coli (strain K12) (see 2 papers)
46% identity, 91% coverage: 6:249/269 of query aligns to 12:256/265 of P07821

query
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P07821

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K50 (= K44) mutation to Q: Lack of activity.
D172 (= D166) mutation to E: Lack of activity.
E173 (= E167) mutation to A: Lack of activity.

5x40A Structure of a cbio dimer bound with amppcp (see paper)
38% identity, 84% coverage: 6:230/269 of query aligns to 5:229/280 of 5x40A

query
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5x40A

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binding phosphomethylphosphonic acid adenylate ester: F14 (≠ Y15), V18 (≠ I18), A20 (≠ I20), N40 (= N40), G41 (= G41), G43 (= G43), K44 (= K44), S45 (= S45), T46 (= T46), Q88 (= Q86), H139 (≠ D140), M140 (≠ T141), L141 (= L142), S142 (= S143), G144 (= G145), Q145 (= Q146), Q166 (≠ E167), H198 (= H199)
binding magnesium ion: S45 (= S45), Q88 (= Q86)

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
33% identity, 84% coverage: 6:230/269 of query aligns to 3:225/241 of 4u00A

query
sites
4u00A

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binding adenosine-5'-diphosphate: F12 (≠ Y15), V17 (≠ I20), S37 (≠ N40), G38 (= G41), S39 (≠ C42), G40 (= G43), K41 (= K44), S42 (= S45), T43 (= T46)

3fvqB Crystal structure of the nucleotide binding domain fbpc complexed with atp (see paper)
36% identity, 87% coverage: 11:245/269 of query aligns to 9:237/350 of 3fvqB

query
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3fvqB

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binding adenosine-5'-triphosphate: F13 (≠ Y15), Q14 (≠ G16), T16 (≠ I18), V18 (≠ I20), S38 (≠ N40), G39 (= G41), C40 (= C42), G41 (= G43), K42 (= K44), T43 (≠ S45), T44 (= T46), R133 (= R137), E137 (≠ T141), S139 (= S143), G141 (= G145), Q142 (= Q146)
binding calcium ion: T43 (≠ S45), Q86 (= Q86)

Q9JI39 ATP-binding cassette sub-family B member 10, mitochondrial; ABC-mitochondrial erythroid protein; ABC-me protein; ATP-binding cassette transporter 10; ABC transporter 10 protein from Mus musculus (Mouse) (see 2 papers)
35% identity, 84% coverage: 18:244/269 of query aligns to 472:701/715 of Q9JI39

query
sites
Q9JI39

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G497 (= G43) mutation to A: Decreases ATP binding about 50%.
K498 (= K44) mutation to R: Decreases ATP binding about 50%.
C547 (≠ L94) modified: S-glutathionyl cysteine; mutation to A: Does not affect ABCB10 glutathionylation.
G602 (= G145) mutation to D: Affects ATP hydrolysis but not binding.; mutation to V: Affects ATP hydrolysis but not binding.
E624 (= E167) mutation to Q: Affects ATP hydrolysis but not binding.
C675 (≠ V220) mutation to A: Prevents ABCB10 glutathionylation.

Sites not aligning to the query:

1:82 modified: transit peptide, Mitochondrion

1vciA Crystal structure of the atp-binding cassette of multisugar transporter from pyrococcus horikoshii ot3 complexed with atp (see paper)
30% identity, 83% coverage: 5:228/269 of query aligns to 6:216/353 of 1vciA

query
sites
1vciA

E

E

L

V

A

K

T

L

R

E

D

N

L

L

S

T

L

K

G

R

Y
x
F

G

G

R

N

I
x
F

T

T

I

A

I

V

D

N

E

K

L

L

A

N

L

L

S

T

L

I

P

K

T

D

G

G

Q

E

V

F

T

L

A

V

I

L

V

L

G

G

P

P

N
x
S

G
|
G

C

C

G
|
G

K
|
K

S
x
T

T
|
T

L

T

L

L

A

R

G

M

L

I

A

A

R

G

L

L

H

E

A

E

P

P

S

T

H

E

G

G

A

R

V

I

L

Y

L

F

D

G

G

D

Q

R

D

D

I

V

Q

T

Q

Y

L

L

P

P

A

P

R

K

E

D

L

-

A

-

R

R

R

N

L

I

A

S

L

M

L

V

P

F

Q

Q

E

H

A

M

S

T

A

V

P

Y

E

E

G

N

L

I

T

A

V

F

A

P

E

-

L

L

V

K

R

K

F

F

G

P

R

K

Q

D

P

E

H

I

Q

D

G

K

W

R

L

V

R

R

Q

-

W

W

S

A

A

A

E

E

D

L

Q

L

Q

Q

V

-

H

-

E

-

A

-

L

-

A

-

D

-

V

I

D

E

E

E

L

L

A

L

D

N

R

R

P

Y

L

P

D

A

T

Q

L

L

S

S

G

G

Q

Q

R

R

Q

Q

R

R

A

V

W

A

I

V

A

A

M

R

T

A

I

I

T

V

Q

V

Q

E

T

P

P

D

L

V

L

L

L

M

D

D

E

E

P

P

T

L

S

S

A

N

L

L

D

D

L

A

G

K

H

L

Q

R

I

V

E

A

V

M

F

R

E

A

L

E

V

I

R

K

L

L

A

Q

H

K

G

L

R

K

-

V

T

T

V

T

V

I

M

Y

V

V

L

T

H

H

D

D

L

Q

A

V

S

E

A

A

C

M

R

T

Y

M

A

G

D

D

H

R

L

I

V

A

A

V

L

M

R

N

H

R

G

G

Q

Q

L

L

V

L

A

Q

A

I

G

G

P

S

P

P

G

T

E

E

V

V

binding adenosine-5'-triphosphate: F16 (≠ Y15), F19 (≠ I18), S41 (≠ N40), G42 (= G41), G44 (= G43), K45 (= K44), T46 (≠ S45), T47 (= T46)

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
29% identity, 83% coverage: 6:228/269 of query aligns to 2:223/240 of 4ymuJ

query
sites
4ymuJ

L

I

A

F

T

V

R

N

D

D

L

V

S

Y

L

K

G

N

Y
x
F

G

G

R

S

I

L

T

E

I
x
V

I

L

D

K

E

G

L

V

A

T

L

L

S

K

L

V

P

N

T

K

G

G

Q

E

V

V

T

V

A

V

I

I

V

I

G

G

P

P

N
x
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

A

R

G

C

L

I

A

N

R

L

L

L

H

E

A

E

P

P

S

T

H

K

G

G

A

E

V

V

L

F

L

I

D

D

G

G

Q

V

D

K

I

I

Q

N

-

N

-

G

Q

K

L

V

P

N

A

I

R

N

E

K

L

V

A

R

R

Q

R

K

L

V

A

G

L

M

L

V

P

F

Q

Q

E

H

A

F

S

N

A

L

P

F

E

P

G

H

L

L

T

T

V

A

A

I

E

E

L

N

V

I

R

T

F

L

G

A

R

P

Q

V

P

K

H

V

Q

K

G

-

W

-

L

-

R

K

Q

M

W

N

S

K

A

K

E

E

D

A

Q

E

V

L

V

A

H

V

E

D

A

L

L

L

A

A

A

K

A

V

D

G

V

L

D

L

E

D

L

K

A

K

D

D

R

Q

P

Y

L

P

D

I

T

K

L

L

S

S

G

G

Q

Q

R

K

Q

Q

R

R

A

L

W

A

I

I

A

A

M

R

T

A

I

L

T

A

Q

M

Q

Q

T

P

P

E

L

V

L

M

L

L

L

F

D

D

E
|
E

P

P

T

T

S

S

A

A

L

L

D

D

L

P

G

E

H

M

Q

V

I

K

E

E

V

V

F

L

E

N

L

V

V

M

R

K

R

Q

L

L

A

A

A

N

H

E

G

G

R

M

T

T

V

M

V

V

M

V

V

V

L

T

H
|
H

D

E

L

M

A

G

S

F

A

A

C

R

R

E

Y

V

A

G

D

D

H

R

L

V

V

I

A

F

L

M

R

D

H

D

G

G

Q

V

L

I

V

V

A

E

G

G

P

T

P

P

G

E

E

E

V

I

binding adenosine-5'-triphosphate: F11 (≠ Y15), V16 (≠ I20), S36 (≠ N40), G37 (= G41), S38 (≠ C42), G39 (= G43), K40 (= K44), S41 (= S45), T42 (= T46), E162 (= E167), H194 (= H199)
binding magnesium ion: S41 (= S45), E162 (= E167)

G7CBF5 Mycobactin import ATP-binding/permease protein IrtA; EC 7.2.2.- from Mycolicibacterium thermoresistibile (strain ATCC 19527 / DSM 44167 / CIP 105390 / JCM 6362 / NCTC 10409 / 316) (Mycobacterium thermoresistibile) (see paper)
36% identity, 86% coverage: 18:247/269 of query aligns to 667:894/908 of G7CBF5

query
sites
G7CBF5

I

V

T

P

I

V

I

I

D

R

E

D

L

V

A

T

L

L

S

T

L

L

P

R

T

P

G

G

Q

T

V

V

T

T

A

A

I

L

V

V

G

G

P

P

N

S

G

G

C

S

G

G

K

K

S

S

T

T

L

L

L

A

A

A

G

L

L

V

A

A

R

R

L

F

H

H

A

D

P

V

S

T

H

Q

G

G

A

A

V

I

L

R

L

V

D

D

G

G

Q

R

D

D

I

I

Q

R

Q

T

L

L

P

T

A

A

R

D

E

E

L

L

A

Y

R

R

L

V

A

G

L

F

L

V

P

L

Q

Q

E

D

A

A

S

Q

A

L

P

V

E

H

G

G

L

-

T

S

V

V

A

A

E

E

L

N

V

I

R

A

F

L

G

A

R

-

Q

E

P

P

H

D

Q

A

G

G

W

L

L

E

R

R

-

I

Q

R

W

T

S

A

A

A

E

R

D

D

Q

A

Q

Q

V

I

V

H

H

D

E

R

A

I

L

T

A

R

A

M

A

P

D

D

V

G

D

Y

E

D

L

S

A

V

D

L

R

G

P

A

L

G

D

S

T

A

L

L

S

S

G

G

Q

E

R

R

Q

Q

R

R

A

V

W

T

I

I

A

A

M

R

T

A

I

I

T

L

Q

A

Q

D

T

T

P

P

L

V

L

L

L

V

L

L

D

D

E

E

P

A

T

T

S

A

A

F

L

A

D

D

L

P

G

E

H

S

Q

E

I

Y

E

L

V

V

F

Q

E

Q

L

A

V

I

R

N

R

R

L

L

A

-

A

T

H

R

G

D

R

R

T

T

V

V

V

L

M

V

V

I

L

A

H

H

D

R

L

L

A

H

S

T

A

I

C

T

R

-

Y

H

A

A

D

D

H

Q

L

I

V

V

A

V

L

L

R

D

H

D

G

G

Q

R

L

I

V

V

A

E

A

V

G

G

P

T

P

H

G

D

E

E

V

L

T

A

-

G

E

G

L

R

V

Y

R

R

Q

G

L

L

Y

W

D

D

I

S

D

G

C

R

T

Y

L

S

I

S

P

P

D

D

Sites not aligning to the query:

70:73 binding FAD
87:91 binding FAD
97:98 binding FAD
241:243 binding FAD

4ayxA Structure of the human mitochondrial abc transporter, abcb10 (rod form b) (see paper)
35% identity, 79% coverage: 18:230/269 of query aligns to 354:567/571 of 4ayxA

query
sites
4ayxA

I

V

T

P

I

I

I

F

D

Q

E

D

L

F

A

S

L

L

S

S

L

I

P

P

T

S

G

G

Q

S

V

V

T

T

A

A

I

L

V

V

G

G

P

P

N
x
S

G
|
G

C

S

G
|
G

K
|
K

S
|
S

T
|
T

L

V

L

L

A

S

G

L

L

L

A

L

R

R

L

L

H

Y

A

D

P

P

S

A

H

S

G

G

A

T

V

I

L

S

L

L

D

D

G

G

Q

H

D

D

I

I

Q

R

Q

Q

L

L

P

N

A

P

R

V

E

W

L

L

A

R

R

S

R

K

L

I

A

G

L

T

L

V

P

S

Q
|
Q

E

E

A

P

S

I

A

L

P

F

E

S

G

-

L

C

T

S

V

I

A

A

E

E

L

N

V

I

R

A

F

Y

G

G

R

A

Q

D

P

D

H

P

Q

S

G

-

W

-

L

-

R

-

Q

S

W

V

S

T

A

A

E

E

D

E

Q

I

Q

Q

V

R

V

V

H

A

E

E

A

V

L

A

A

N

A

A

A

V

D

A

V

F

D

I

E

R

L

N

A

F

D

P

R

Q

P

G

L

F

D

N

T

T

-

V

-

G

-

E

-

K

-

G

-

V

-

L

L

L

S

S

G

G

Q

Q

R

K

Q

Q

R

R

A

I

W

A

I

I

A

A

M

R

T

A

I

L

T

L

Q

K

Q

N

T

P

P

K

L

I

L

L

D

D

E

E

P

A

T

T

S

S

A

A

L

L

D

D

L

A

G

E

H

N

Q

E

I

Y

E

L

V

V

F

Q

E

E

L

A

V

L

R

D

R

R

L

L

A

-

A

M

H

D

G

G

R

R

T

T

V

V

V

L

M

V

V

I

L

A

H

H

D

R

L

L

A

-

S

S

A

T

C

I

R

K

Y

N

A

A

D

N

H

M

L

V

V

A

A

V

L

L

R

D

H

Q

G

G

Q

K

L

I

V

T

A

E

A

Y

G

G

P

K

P

H

G

E

E

E

V

L

T

S

binding phosphomethylphosphonic acid adenylate ester: S376 (≠ N40), G377 (= G41), G379 (= G43), K380 (= K44), S381 (= S45), T382 (= T46)
binding magnesium ion: S381 (= S45), Q422 (= Q86)

Sites not aligning to the query:

7y48B Cryo-em structure of biliverdin-bound mitochondrial abc transporter abcb10 from biortus (see paper)
35% identity, 79% coverage: 18:230/269 of query aligns to 353:563/567 of 7y48B

query
sites
7y48B

I

V

T

P

I

I

I

F

D

Q

E

D

L

F

A

S

L

L

S

S

L

I

P

P

T

S

G

G

Q

S

V

V

T

T

A

A

I

L

V

V

G

G

P

P

N

S

G

G

C

S

G

G

K

K

S

S

T

T

L

V

L

L

A

S

G

L

L

L

A

L

R

R

L

L

H

Y

A

D

P

P

S

A

H

S

G

G

A

T

V

I

L

S

L

L

D

D

G

G

Q

H

D

D

I

I

Q

R

Q

Q

L

L

P

N

A

P

R

V

E

W

L

L

A

R

R

S

R

K

L

I

A

G

L

T

L

V

P

S

Q

Q

E

E

A

P

S

I

A

L

P

F

E

S

G

-

L

C

T

S

V

I

A

A

E

E

L

N

V

I

R

A

F

Y

G

G

R

A

Q

D

P

D

H

P

Q

S

G

-

W

-

L

-

R

-

Q

S

W

V

S

T

A

A

E

E

D

E

Q

I

Q

Q

V

R

V

V

H

A

E

E

A

V

L

A

A

N

A

A

A

V

D

A

V

F

D

I

E

R

L

N

A

F

D

P

R

Q

P

G

L

F

D

N

T

T

-

V

-

G

-

V

-

L

L

L

S

S

G

G

Q

Q

R

K

Q

Q

R

R

A

I

W

A

I

I

A

A

M

R

T

A

I

L

T

L

Q

K

Q

N

T

P

P

K

L

I

L

L

D

D

E

Q

P

A

T

T

S

S

A

A

L

L

D

D

L

A

G

E

H

N

Q

E

I

Y

E

L

V

V

F

Q

E

E

L

A

V

L

R

D

R

R

L

L

A

-

A

M

H

D

G

G

R

R

T

T

V

V

V

L

M

V

V

I

L

A

H

H

D

R

L

L

A

-

S

S

A

T

C

I

R

K

Y

N

A

A

D

N

H

M

L

V

V

A

A

V

L

L

R

D

H

Q

G

G

Q

K

L

I

V

T

A

E

A

Y

G

G

P

K

P

H

G

E

E

E

V

L

T

S

Sites not aligning to the query:

binding Biliverdine IX Alpha: 171, 248, 252, 255

Q9NRK6 ATP-binding cassette sub-family B member 10, mitochondrial; ABC-mitochondrial erythroid protein; ABC-me protein; ATP-binding cassette transporter 10; ABC transporter 10 protein; Mitochondrial ATP-binding cassette 2; M-ABC2 from Homo sapiens (Human) (see 5 papers)
34% identity, 83% coverage: 18:239/269 of query aligns to 507:731/738 of Q9NRK6

query
sites
Q9NRK6

I

V

T

P

I

I

I

F

D

Q

E

D

L

F

A

S

L

L

S

S

L

I

P

P

T

S

G

G

Q

S

V

V

T

T

A

A

I

L

V

V

G

G

P

P

N

S

G

G

C

S

G

G

K
|
K

S

S

T

T

L

V

L

L

A

S

G

L

L

L

A

L

R

R

L

L

H

Y

A
x
D

P

P

S

A

H

S

G

G

A

T

V

I

L

S

L

L

D

D

G

G

Q

H

D

D

I

I

Q

R

Q

Q

L

L

P

N

A

P

R

V

E

W

L

L

A

R

R

S

R

K

L

I

A

G

L

T

L

V

P

S

Q

Q

E

E

A

P

S

I

A

L

P

-

E

F

G

S

L
x
C

T

S

V

I

A

A

E

E

L

N

V

I

R

A

F

Y

G

G

R

A

Q

D

P

D

H

P

Q

S

G

-

W

-

L

-

R

-

Q

S

W

V

S

T

A

A

E

E

D

E

Q

I

Q

Q

V

R

V

V

H

A

E

E

A

V

L

A

A

N

A

A

A

V

D

A

V

F

D

I

E

R

L

N

A

F

D

P

R

Q

P

G

L

F

D

N

T

T

-

V

-

G

-

E

-

K

-

G

-

V

-

L

L

L

S
|
S

G

G

Q
|
Q

R

K

Q

Q

R

R

A

I

W

A

I

I

A

A

M

R

T

A

I

L

T

L

Q

K

Q

N

T

P

P

K

L

I

L

L

D
|
D

E
|
E

P

A

T

T

S

S

A

A

L

L

D

D

L

A

G

E

H

N

Q

E

I

Y

E

L

V

V

F

Q

E

E

L

A

V

L

R

D

R

R

L

L

A

-

A

M

H

D

G

G

R

R

T

T

V

V

V

L

M

V

V

I

L

A

H

H

D

R

L

L

A

-

S

S

A

T

C

I

R

K

Y

N

A

A

D

N

H

M

L

V

V

A

A

V

L

L

R

D

H

Q

G

G

Q

K

L

I

V

T

A

E

A

Y

G

G

P

K

P

H

G

E

E

E

V

L

T

S

-

K

-

P

T

N

E

G

L

I

V

Y

R

R

Q

K

L

L

Y

M

D

N

K533 (= K44) mutation to E: Increases hemoglobin biosynthetic process.
D545 (≠ A56) to N: in dbSNP:rs35698797
C582 (≠ L94) mutation to G: Does not affect ATPase activity; when associated with S-215 and L-224. Activated by Zn (II) mesoporphyrin; when associated with S-215 and L-224.
S635 (= S143) mutation to R: Does not rescue hemoglobin and heme biosynthetic process.
Q638 (= Q146) mutation to H: Does not rescue hemoglobin and heme biosynthetic process.
D658 (= D166) mutation to A: Does not rescue hemoglobin and heme biosynthetic process.
E659 (= E167) mutation to A: Does not rescue hemoglobin and heme biosynthetic process.

Sites not aligning to the query:

150 A → S: in dbSNP:rs4148756
215 C→S: Does not affect ATPase activity; when associated with L-224 and G-582. Activated by Zn (II) mesoporphyrin; when associated with L-224 and G-582.
224 C→L: Does not affect ATPase activity; when associated withS-215 and G-582. Activated by Zn (II) mesoporphyrin; when associated with S-215 and G-582.
471 R → T: in a breast cancer sample; somatic mutation

P69874 Spermidine/putrescine import ATP-binding protein PotA; EC 7.6.2.11 from Escherichia coli (strain K12) (see 3 papers)
35% identity, 78% coverage: 20:228/269 of query aligns to 32:235/378 of P69874

query
sites
P69874

I

V

I

I

D

P

E

Q

L

L

A

D

L

L

S

T

L

I

P

N

T

N

G

G

Q

E

V
x
F

T

L

A

T

I

L

V

L

G

G

P

P

N

S

G

G

C
|
C

G

G

K

K

S

T

T

T

L

V

L
|
L

A

R

G

L

L

I

A

A

R

G

L

L

H

E

A

T

P

V

S

D

H

S

G

G

A

R

V

I

L

M

L
|
L

D

D

G

N

Q

E

D

D

I

I

Q

T

Q

H

L

V

P

P

A

A

R

E

E

N

L

-

A

-

R

R

R

Y

L

V

A

N

L

T

L

V

P

F

Q

Q

E

S

A

Y

S

A

A

L

P

F

E

P

G

H

L

M

T

T

V

V

A

F

E

E

L

N

V

V

R

A

F

F

G

G

R

L

Q

R

P

M

H

Q

Q

-

G

-

W

-

L

-

R

K

Q

T

W

P

S

A

A

A

E

E

D

I

Q

T

Q

P

V

R

V

V

H

M

E

E

A

A

L

L

A

R

A

M

A
x
V

D

Q

V

L

D

E

E

T

L

F

A

A

D

Q

R

R

P

K

L

P

D

H

T

Q

L

L

S

S

G

G

Q

Q

R

Q

Q

Q

R

R

A

V

W

A

I

I

A

A

M

R

T

A

I

V

T

V

Q

N

Q

K

T

P

P

R

L

L

D
|
D

E

E

P

S

T

L

S

S

A

A

L

L

D

D

L

Y

G

K

-

L

-

R

-

K

-

Q

H

M

Q

Q

I

N

E

E

V

L

F

K

E

A

L

L

V

Q

R

R

R

K

L

L

A

-

H

-

G

G

R

I

T

T

V

F

V

V

M

F

V

V

L

T

H

H

D

D

L

Q

A

E

S

E

A

A

C

L

R

T

Y

M

A

S

D

D

H

R

L

I

V

V

A

V

L

M

R

R

H

D

G

G

Q

R

L

I

V

E

A

Q

A

D

G

G

P

T

P

P

G

R

E

E

V

I

F45 (≠ V33) mutation to L: Lower ATPase activity and transport efficiency.
C54 (= C42) mutation to T: Loss of ATPase activity and transport.
L60 (= L48) mutation to F: Lower ATPase activity and transport efficiency.
L76 (= L64) mutation to P: Lower ATPase activity and transport efficiency.
V135 (≠ A129) mutation to M: Loss of ATPase activity and transport.
D172 (= D166) mutation to N: Loss of ATPase activity and transport.

Sites not aligning to the query:

26 C→A: Lower ATPase activity and transport efficiency.
27 F→L: Lower ATPase activity and transport efficiency.
276 C→A: Lower ATPase activity and transport efficiency.
297 mutation E->K,D: Lower ATPase activity and transport efficiency.; E→Q: Loss of ATPase activity and transport.

P9WQJ9 Mycobactin import ATP-binding/permease protein IrtA; Iron-regulated transporter A; EC 7.2.2.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
37% identity, 79% coverage: 11:223/269 of query aligns to 615:825/859 of P9WQJ9

query
sites
P9WQJ9

L

V

S

T

L

F

G

G

Y

Y

-

R

G

P

R

G

I

V

T

P

I

V

I

I

D

Q

E

D

L

V

A

S

L

L

S

T

L

L

P

R

T

P

G

G

Q

T

V

V

T

T

A

A

I

L

V

V

G

G

P

P

N

S

G

G

C

S

G

G

K

K

S

S

T

T

L

L

L

A

A

T

G

L

L

L

A

A

R

R

L

F

H

H

A

D

P

V

S

E

H

R

G

G

A

A

V

I

L

R

L

V

D

G

G

G

Q

Q

D

D

I

I

Q

R

Q

S

L

L

P

A

A

A

R

D

E

E

L

L

A

Y

R

T

R

R

L

V

A

G

L

F

L

V

P

L

Q

Q

E

E

A

A

S

Q

A

L

P

V

E

H

G

G

L

-

T

T

V

A

A

A

E

E

L

N

V

I

R

A

F

L

G

A

R

V

Q

P

P

D

H

A

Q

P

G

A

W

E

L

Q

R

V

Q

Q

W

V

S

A

A

A

E

R

D

E

Q

A

Q

Q

V

I

V

H

H

D

E

R

A

V

L

L

A

R

A

L

A

P

D

D

V

G

D

Y

E

D

L

T

A

V

D

L

R

G

P

A

L

N

D

S

T

G

L

L

S

S

G

G

Q

E

R

R

Q

Q

R

R

A

L

W

T

I

I

A

A

M

R

T

A

I

I

T

L

Q

G

Q

D

T

T

P

P

L

V

L

L

L

I

L

L

D

D

E

E

P

A

T

T

S

A

A

F

L

A

D

D

L

P

G

E

H

S

Q

E

I

Y

E

L

V

V

F

Q

E

Q

L

A

V

L

R

N

R

R

L

L

A

-

A

T

H

R

G

D

R

R

T

T

V

V

V

L

M

V

V

I

L

A

H

H

D

R

L

L

A

H

S

T

A

I

C

T

R

R

Y

-

A

A

D

D

H

Q

L

I

V

V

A

V

L

L

R

D

H

H

G

G

Q

R

L

I

V

V

A

E

A

R

G

G

Sites not aligning to the query:

70 R→A: No change in FAD-binding and in activity.
72 Y→A: Decrease in FAD-binding and loss of activity.
73 T→A: Decrease in FAD-binding and loss of activity.

7wixA Cryo-em structure of mycobacterium tuberculosis irtab in complex with adp (see paper)
37% identity, 79% coverage: 18:229/269 of query aligns to 348:556/571 of 7wixA

query
sites
7wixA

I

V

T

P

I
x
V

I

I

D

Q

E

D

L

V

A

S

L

L

S

T

L

L

P

R

T

P

G

G

Q

T

V

V

T

T

A

A

I

L

V

V

G

G

P

P

N
x
S

G
|
G

C

S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

A

A

T

G

L

L

L

A

A

R

R

L

F

H

H

A

D

P

V

S

E

H

R

G

G

A

A

V

I

L

R

L

V

D

G

G

G

Q

Q

D

D

I

I

Q

R

Q

S

L

L

P

A

A

A

R

D

E

E

L

L

A

Y

R

T

R

R

L

V

A

G

L

F

L

V

P

L

Q

Q

E

E

A

A

S

Q

A

L

P

V

E

H

G

G

L

-

T

T

V

A

A

A

E

E

L

N

V

I

R

A

F

L

G

A

R

V

Q

P

P

D

H

A

Q

P

G

A

W

E

L

Q

R

V

Q

Q

W

V

S

A

A

A

E

R

D

E

Q

A

Q

Q

V

I

V

H

H

D

E

R

A

V

L

L

A

R

A

L

A

P

D

D

V

G

D

Y

E

D

L

T

A

V

D

L

R

G

P

A

L

N

D

S

T

G

L

L

S

S

G

G

Q

E

R

R

Q

Q

R

R

A

L

W

T

I

I

A

A

M

R

T

A

I

I

T

L

Q

G

Q

D

T

T

P

P

L

V

L

L

L

I

L

L

D

D

E

E

P

A

T

T

S

A

A

F

L

A

D

D

L

P

G

E

H

S

Q

E

I

Y

E

L

V

V

F

Q

E

Q

L

A

V

L

R

N

R

R

L

L

A

-

A

T

H

R

G

D

R

R

T

T

V

V

V

L

M

V

V

I

L

A

H

H

D

R

L

L

A

H

S

T

A

I

C

T

R

R

Y

-

A

A

D

D

H

Q

L

I

V

V

A

V

L

L

R

D

H

H

G

G

Q

R

L

I

V

V

A

E

A

R

G

G

P

T

P

H

G

E

E

E

V

L

binding adenosine-5'-diphosphate: V350 (≠ I20), S370 (≠ N40), G371 (= G41), G373 (= G43), K374 (= K44), S375 (= S45), T376 (= T46)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 344

7wivA Cryo-em structure of mycobacterium tuberculosis irtab in complex with an amp-pnp (see paper)
37% identity, 79% coverage: 18:229/269 of query aligns to 348:556/571 of 7wivA

query
sites
7wivA

I

V

T

P

I
x
V

I

I

D

Q

E

D

L

V

A

S

L

L

S

T

L

L

P

R

T

P

G

G

Q

T

V

V

T

T

A

A

I

L

V

V

G

G

P

P

N
x
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

A

A

T

G

L

L

L

A

A

R

R

L

F

H

H

A

D

P

V

S

E

H

R

G

G

A

A

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L

V

D

G

G

G

Q

Q

D

D

I

I

Q

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S

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A

A

A

R

D

E

E

L

L

A

Y

R

T

R

R

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A

G

L

F

L

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P

L

Q

Q

E

E

A

A

S

Q

A

L

P

V

E

H

G

G

L

-

T

T

V

A

A

A

E

E

L

N

V

I

R

A

F

L

G

A

R

V

Q

P

P

D

H

A

Q

P

G

A

W

E

L

Q

R

V

Q

Q

W

V

S

A

A

A

E

R

D

E

Q

A

Q

Q

V

I

V

H

H

D

E

R

A

V

L

L

A

R

A

L

A

P

D

D

V

G

D

Y

E

D

L

T

A

V

D

L

R

G

P

A

L

N

D

S

T

G

L

L

S

S

G

G

Q

E

R

R

Q

Q

R

R

A

L

W

T

I

I

A

A

M

R

T

A

I

I

T

L

Q

G

Q

D

T

T

P

P

L

V

L

L

L

I

L

L

D

D

E

E

P

A

T

T

S

A

A

F

L

A

D

D

L

P

G

E

H

S

Q

E

I

Y

E

L

V

V

F

Q

E

Q

L

A

V

L

R

N

R

R

L

L

A

-

A

T

H

R

G

D

R

R

T

T

V

V

V

L

M

V

V

I

L

A

H

H

D

R

L

L

A

H

S

T

A

I

C

T

R

R

Y

-

A

A

D

D

H

Q

L

I

V

V

A

V

L

L

R

D

H

H

G

G

Q

R

L

I

V

V

A

E

A

R

G

G

P

T

P

H

G

E

E

E

V

L

binding phosphoaminophosphonic acid-adenylate ester: V350 (≠ I20), S370 (≠ N40), G371 (= G41), S372 (≠ C42), G373 (= G43), K374 (= K44), S375 (= S45), T376 (= T46)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 344

7wixB Cryo-em structure of mycobacterium tuberculosis irtab in complex with adp (see paper)
37% identity, 82% coverage: 10:230/269 of query aligns to 333:553/576 of 7wixB

query
sites
7wixB

D

D

L

V

S

A

L

F

G

G

Y

Y

-

D

-

G

G

G

R

S

I

G

T

P

I

V

I

L

D

D

E

G

L

V

A

S

L

F

S

C

L

L

P

Q

T

P

G

G

Q

T

V

T

T

T

A

A

I

I

V

V

G

G

P

P

N

S

G
|
G

C

C

G
|
G

K
|
K

S
|
S

T

T

L

I

L

L

A

A

G

L

L

I

A

A

R

G

L

L

H

H

A

Q

P

P

S

T

H

R

G

G

A

R

V

V

L

L

L

I

D

D

G

G

Q

T

D

D

I

V

Q

A

Q

T

L

L

P

D

A

A

R

R

E

A

L

Q

A

Q

R

A

R

V

L

C

A

S

L

V

P

F

Q
|
Q

E

H

A

P

S

Y

A

L

P

F

E

H

G

G

L

-

T

T

V

I

A

R

E

D

L

N

V

V

R

-

F

F

G

A

R

A

Q

D

P

P

H

-

Q

-

G

-

W

-

L

-

R

-

Q

-

W

-

S

G

A

A

E

S

D

D

Q

D

Q

Q

V

F

V

A

H

-

E

Q

A

A

L

V

A

R

A

L

A

A

D

R

V

V

D

D

E

E

L

L

A

I

D

A

R

R

P

L

L

P

D

D

-

G

-

A

-

N

-

T

-

I

-

V

-

G

-

E

-

A

-

G

-

S

T

A

L

L

S

S

G

G

Q

E

R

R

Q

Q

R

R

A

V

W

S

I

I

A

A

M

R

T

A

I

L

T

L

Q

K

Q

A

T

A

P

P

L

V

L

L

L

V

D

D

E

E

P

A

T

T

S

S

A

A

L

L

D

D

L

A

G

E

H

N

Q

E

I

A

E

A

V

V

F

-

E

V

L

D

V

A

R

L

R

A

L

A

A

D

A

P

H

R

G

S

R

R

T

T

V

R

V

V

M

I

V

V

L

A

H

H

D

R

L

L

A

A

S

S

A

-

C

I

R

R

Y

H

A

A

D

D

H

R

L

V

V

L

A

F

L

V

R

D

H

D

G

G

Q

R

L

V

V

V

A

E

A

D

G

G

P

S

P

I

G

S

E

E

V

L

T

T

binding adenosine-5'-diphosphate: G366 (= G41), G368 (= G43), K369 (= K44), S370 (= S45)
binding magnesium ion: S370 (= S45), Q411 (= Q86)

8y5iA Cryo-em structure of e.Coli spermidine transporter potd-potabc in translocation intermidiate state (see paper)
35% identity, 78% coverage: 20:228/269 of query aligns to 17:220/358 of 8y5iA

query
sites
8y5iA

I

V

I

I

D

P

E

Q

L

L

A

D

L

L

S

T

L

I

P

N

T

N

G

G

Q

E

V

F

T

L

A

T

I

L

V

L

G

G

P

P

N

S

G
|
G

C

C

G

G

K
|
K

S
x
T

T
|
T

L

V

L

L

A

R

G

L

L

I

A

A

R

G

L

L

H

E

A

T

P

V

S

D

H

S

G

G

A

R

V

I

L

M

L

L

D

D

G

N

Q

E

D

D

I

I

Q

T

Q

H

L

V

P

P

A

A

R

E

E

N

L

-

A

-

R

R

R

Y

L

V

A

N

L

T

L

V

P

F

Q

Q

E

S

A

Y

S

A

A

L

P

F

E

P

G

H

L

M

T

T

V

V

A

F

E

E

L

N

V

V

R

A

F

F

G

G

R

L

Q

R

P

M

H

Q

Q

-

G

-

W

-

L

-

R

K

Q

T

W

P

S

A

A

A

E

E

D

I

Q

T

Q

P

V

R

V

V

H

M

E

E

A

A

L

L

A

R

A

M

A

V

D

Q

V

L

D

E

E

T

L

F

A

A

D

Q

R
|
R

P

K

L

P

D

H

T
x
Q

L

L

S
|
S

G

G

Q

Q

R

Q

Q

Q

R

R

A

V

W

A

I

I

A

A

M

R

T

A

I

V

T

V

Q

N

Q

K

T

P

P

R

L

L

D

D

E

Q

P

S

T

L

S

S

A

A

L

L

D

D

L

Y

G

K

-

L

-

R

-

K

-

Q

H

M

Q

Q

I

N

E

E

V

L

F

K

E

A

L

L

V

Q

R

R

R

K

L

L

A

-

H

-

G

G

R

I

T

T

V

F

V

V

M

F

V

V

L

T

H

H

D

D

L

Q

A

E

S

E

A

A

C

L

R

T

Y

M

A

S

D

D

H

R

L

I

V

V

A

V

L

M

R

R

H

D

G

G

Q

R

L

I

V

E

A

Q

A

D

G

G

P

T

P

P

G

R

E

E

V

I

binding adenosine-5'-triphosphate: G38 (= G41), K41 (= K44), T42 (≠ S45), T43 (= T46), R128 (= R137), Q132 (≠ T141), S134 (= S143)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 12, 15

7wiwA Cryo-em structure of mycobacterium tuberculosis irtab complexed with atp in an occluded conformation (see paper)
37% identity, 79% coverage: 18:229/269 of query aligns to 348:556/570 of 7wiwA

query
sites
7wiwA

I

V

T

P

I
x
V

I

I

D

Q

E

D

L

V

A

S

L

L

S

T

L

L

P

R

T

P

G

G

Q

T

V

V

T

T

A

A

I

L

V

V

G

G

P

P

N
x
S

G

G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

A

A

T

G

L

L

L

A

A

R

R

L

F

H

H

A

D

P

V

S

E

H

R

G

G

A

A

V

I

L

R

L

V

D

G

G

G

Q

Q

D

D

I

I

Q

R

Q

S

L

L

P

A

A

A

R

D

E

E

L

L

A

Y

R

T

R

R

L

V

A

G

L

F

L

V

P

L

Q
|
Q

E

E

A

A

S

Q

A

L

P

V

E

H

G

G

L

-

T

T

V

A

A

A

E

E

L

N

V

I

R

A

F

L

G

A

R

V

Q

P

P

D

H

A

Q

P

G

A

W

E

L

Q

R

V

Q

Q

W

V

S

A

A

A

E

R

D

E

Q

A

Q

Q

V

I

V

H

H

D

E
x
R

A

V

L

L

A

R

A

L

A

P

D

D

V

G

D

Y

E

D

L

T

A

V

D

L

R

G

P

A

L

N

D

S

T
x
G

L

L

S
|
S

G

G

G
|
G

Q
x
E

R

R

Q

Q

R

R

A

L

W

T

I

I

A

A

M

R

T

A

I

I

T

L

Q

G

Q

D

T

T

P

P

L

V

L

L

L

I

L

L

D

D

E

Q

P

A

T

T

S

A

A

F

L

A

D

D

L

P

G

E

H

S

Q

E

I

Y

E

L

V

V

F

Q

E

Q

L

A

V

L

R

N

R

R

L

L

A

-

A

T

H

R

G

D

R

R

T

T

V

V

V

L

M

V

V

I

L

A

H
|
H

D

R

L

L

A

H

S

T

A

I

C

T

R

R

Y

-

A

A

D

D

H

Q

L

I

V

V

A

V

L

L

R

D

H

H

G

G

Q

R

L

I

V

V

A

E

A

R

G

G

P

T

P

H

G

E

E

E

V

L

binding adenosine-5'-triphosphate: V350 (≠ I20), S370 (≠ N40), S372 (≠ C42), G373 (= G43), K374 (= K44), S375 (= S45), T376 (= T46), Q416 (= Q86), R453 (≠ E124), G470 (≠ T141), S472 (= S143), G474 (= G145), E475 (≠ Q146), H527 (= H199)
binding magnesium ion: S375 (= S45), Q416 (= Q86)

Sites not aligning to the query:

3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
32% identity, 82% coverage: 9:228/269 of query aligns to 6:220/371 of 3puyA

query
sites
3puyA

R

Q

D

N

L

V

S

T

L

K

G

A

Y
x
W

G

G

R

E

I

V

T

V

I

V

I

S

D

K

E

D

L

I

A

N

L

L

S

D

L

I

P

H

T

E

G

G

Q

E

V

F

T

V

A

V

I

F

V

V

G

G

P

P

N
x
S

G
|
G

C
|
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

A

R

G

M

L

I

A

A

R

G

L

L

H

E

A

T

P

I

S

T

H

S

G

G

A

D

V

L

L

F

L

I

D

G

G

E

Q

K

D

R

I

M

Q

N

Q

D

L

T

P

P

A

P

R

A

E

E

L

-

A

-

R

R

R

G

L

V

A

G

L

M

L

V

P

F

Q
|
Q

E

S

A

Y

S

A

A

L

P

Y

E

P

G

H

L

L

T

S

V

V

A

A

E

E

L

N

V

M

R

S

F

F

G

G

R

-

Q

-

P

-

H

-

Q

-

G

-

W

-

L

L

R

K

Q

L

W

A

S

G

A

A

E

K

D

K

Q

E

Q

V

V

I

V

N

H

Q

E

R

A

V

L

N

A

Q

A

V

A

A

D

E

V

V

D

L

E

Q

L

L

A

A

-

H

-

L

D

D

R
|
R

P

K

L

P

D

K

T
x
A

L

L

S
|
S

G

G

G
|
G

Q
|
Q

R

R

Q

Q

R

R

A

V

W

A

I

I

A

G

M

R

T

T

I

L

T

V

Q

A

Q

E

T

P

P

S

L

V

L

F

L

L

D

D

E
|
E

P

P

T

L

S

S

A

N

L

L

D

D

-

A

-

L

-

R

L

V

G

Q

H

M

Q

R

I

I

E

E

V

I

F

S

E

R

L

L

V

H

R

K

R

R

L

L

A

-

H

-

G

G

R

R

T

T

V

M

V

I

M

Y

V

V

L

T

H
|
H

D

D

L

Q

A

V

S

E

A

A

C

M

R

T

Y

L

A

A

D

D

H

K

L

I

V

V

A

V

L

L

R

D

H

A

G

G

Q

R

L

V

V

A

A

Q

A

V

G

G

P

K

P

P

G

L

E

E

V

L

binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ Y15), S37 (≠ N40), G38 (= G41), C39 (= C42), G40 (= G43), K41 (= K44), S42 (= S45), T43 (= T46), Q81 (= Q86), R128 (= R137), A132 (≠ T141), S134 (= S143), G136 (= G145), Q137 (= Q146), E158 (= E167), H191 (= H199)
binding magnesium ion: S42 (= S45), Q81 (= Q86)

3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
32% identity, 82% coverage: 9:228/269 of query aligns to 6:220/371 of 3puxA

query
sites
3puxA

R

Q

D

N

L

V

S

T

L

K

G

A

Y
x
W

G

G

R

E

I

V

T

V

I

V

I

S

D

K

E

D

L

I

A

N

L

L

S

D

L

I

P

H

T

E

G

G

Q

E

V

F

T

V

A

V

I

F

V

V

G

G

P

P

N
x
S

G
|
G

C
|
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

A

R

G

M

L

I

A

A

R

G

L

L

H

E

A

T

P

I

S

T

H

S

G

G

A

D

V

L

L

F

L

I

D

G

G

E

Q

K

D

R

I

M

Q

N

Q

D

L

T

P

P

A

P

R

A

E

E

L

-

A

-

R

R

R

G

L

V

A

G

L

M

L

V

P

F

Q
|
Q

E

S

A

Y

S

A

A

L

P

Y

E

P

G

H

L

L

T

S

V

V

A

A

E

E

L

N

V

M

R

S

F

F

G

G

R

-

Q

-

P

-

H

-

Q

-

G

-

W

-

L

L

R

K

Q

L

W

A

S

G

A

A

E

K

D

K

Q

E

Q

V

V

I

V

N

H

Q

E

R

A

V

L

N

A

Q

A

V

A

A

D

E

V

V

D

L

E

Q

L

L

A

A

-

H

-

L

D

D

R
|
R

P

K

L

P

D

K

T

A

L

L

S
|
S

G

G

G
|
G

Q
|
Q

R

R

Q

Q

R

R

A

V

W

A

I

I