SitesBLAST
Comparing WP_086509633.1 NCBI__GCF_002151265.1:WP_086509633.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3bptA Crystal structure of human beta-hydroxyisobutyryl-coa hydrolase in complex with quercetin
34% identity, 92% coverage: 17:370/384 of query aligns to 13:352/362 of 3bptA
- active site: G67 (= G71), P84 (≠ E97), R88 (≠ T101), G115 (= G128), G118 (= G131), E138 (= E151), D146 (= D159)
- binding (2r)-3-hydroxy-2-methylpropanoic acid: G66 (= G70), G67 (= G71), I69 (≠ V73), E90 (= E103), G114 (= G127), G115 (= G128), E138 (= E151), D146 (= D159), V147 (≠ I160)
- binding 3,5,7,3',4'-pentahydroxyflavone: F25 (≠ S29), L26 (= L30), A28 (= A32), G66 (= G70), G67 (= G71), I69 (≠ V73), P137 (= P150), I141 (= I154), L319 (= L337)
Q9LKJ1 3-hydroxyisobutyryl-CoA hydrolase 1; CoA-thioester hydrolase CHY1; EC 3.1.2.-; EC 3.1.2.4 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
33% identity, 90% coverage: 19:364/384 of query aligns to 19:354/378 of Q9LKJ1
- G70 (= G71) mutation to S: Loss of activity.
- E142 (= E151) mutation to A: Loss of activity.
- D150 (= D159) mutation to G: Reduced activity.
4hdtA Crystal structure of a carnitinyl-coa dehydratase from mycobacterium thermoresistibile (see paper)
37% identity, 93% coverage: 9:366/384 of query aligns to 3:331/340 of 4hdtA
- active site: G64 (= G71), I69 (≠ L76), W84 (≠ F100), Y88 (= Y104), G112 (= G128), G115 (= G131), E135 (= E151), P142 (= P158), D143 (= D159), R283 (= R314)
- binding zinc ion: H28 (≠ L35), E42 (≠ A49), E57 (= E64), E79 (≠ N86), H93 (≠ R109), H185 (≠ R201)
Sites not aligning to the query:
6z1pBI mS93 (see paper)
28% identity, 47% coverage: 14:195/384 of query aligns to 27:201/1413 of 6z1pBI
- active site: T85 (≠ G71), S134 (≠ G128), E157 (= E151), D165 (= D159)
- binding : Y41 (≠ K28), K42 (≠ S29), Q43 (≠ L30), T45 (≠ A32), D47 (≠ S34), H49 (≠ D36), K83 (≠ A69), T85 (≠ G71), D86 (= D72), F87 (≠ V73), K88 (≠ V74), K92 (≠ R78), L130 (≠ I124), K152 (≠ L146)
Sites not aligning to the query:
5zaiC Crystal structure of 3-hydroxypropionyl-coa dehydratase from metallosphaera sedula (see paper)
29% identity, 50% coverage: 8:200/384 of query aligns to 3:183/259 of 5zaiC
- active site: A65 (≠ G71), F70 (≠ L76), S82 (≠ R87), R86 (= R91), G110 (= G128), E113 (≠ G131), P132 (= P150), E133 (= E151), I138 (≠ L156), P140 (= P158), G141 (≠ D159)
- binding coenzyme a: K24 (≠ S29), L25 (= L30), A63 (= A69), G64 (= G70), A65 (≠ G71), D66 (= D72), I67 (≠ V73), P132 (= P150), R166 (≠ Q183)
Sites not aligning to the query:
P14604 Enoyl-CoA hydratase, mitochondrial; mECH; mECH1; Enoyl-CoA hydratase 1; ECHS1; Short-chain enoyl-CoA hydratase; SCEH; EC 4.2.1.17; EC 5.3.3.8 from Rattus norvegicus (Rat) (see 3 papers)
31% identity, 54% coverage: 8:215/384 of query aligns to 33:227/290 of P14604
- E144 (≠ G131) mutation to D: Reduces activity 50-fold.; mutation to Q: Reduces activity 3300-fold.
- E164 (= E151) mutation to D: Reduces activity 1250-fold.; mutation to Q: Reduces activity 330000-fold.
Sites not aligning to the query:
- 1:29 modified: transit peptide, Mitochondrion
2dubA Enoyl-coa hydratase complexed with octanoyl-coa (see paper)
30% identity, 54% coverage: 8:215/384 of query aligns to 2:191/254 of 2dubA
- active site: A67 (≠ G71), M72 (≠ G84), S82 (≠ F100), G105 (= G128), E108 (≠ G131), P127 (= P150), E128 (= E151), T133 (≠ L156), P135 (= P158), G136 (≠ D159)
- binding octanoyl-coenzyme a: K25 (= K28), A26 (≠ S29), L27 (= L30), A29 (= A32), A65 (= A69), A67 (≠ G71), D68 (= D72), I69 (≠ V73), K70 (≠ E82), G105 (= G128), E108 (≠ G131), P127 (= P150), E128 (= E151), G136 (≠ D159), A137 (≠ I160)
Sites not aligning to the query:
1ey3A Structure of enoyl-coa hydratase complexed with the substrate dac-coa (see paper)
30% identity, 54% coverage: 8:215/384 of query aligns to 1:195/258 of 1ey3A
- active site: A66 (≠ G71), M71 (≠ G84), S81 (vs. gap), L85 (≠ T101), G109 (= G128), E112 (≠ G131), P131 (= P150), E132 (= E151), T137 (≠ L156), P139 (= P158), G140 (≠ D159)
- binding 4-(n,n-dimethylamino)cinnamoyl-coa: K24 (= K28), L26 (= L30), A28 (= A32), A64 (= A69), G65 (= G70), A66 (≠ G71), D67 (= D72), I68 (≠ V73), L85 (≠ T101), W88 (≠ Y104), G109 (= G128), P131 (= P150), L135 (≠ I154), G140 (≠ D159)
Sites not aligning to the query:
1dubA 2-enoyl-coa hydratase, data collected at 100 k, ph 6.5 (see paper)
30% identity, 54% coverage: 8:215/384 of query aligns to 3:197/260 of 1dubA
- active site: A68 (≠ G71), M73 (≠ G84), S83 (vs. gap), L87 (≠ T101), G111 (= G128), E114 (≠ G131), P133 (= P150), E134 (= E151), T139 (≠ L156), P141 (= P158), G142 (≠ D159)
- binding acetoacetyl-coenzyme a: K26 (= K28), A27 (≠ S29), L28 (= L30), A30 (= A32), A66 (= A69), A68 (≠ G71), D69 (= D72), I70 (≠ V73), Y107 (≠ I124), G110 (= G127), G111 (= G128), E114 (≠ G131), P133 (= P150), E134 (= E151), L137 (≠ I154), G142 (≠ D159)
Sites not aligning to the query:
1mj3A Crystal structure analysis of rat enoyl-coa hydratase in complex with hexadienoyl-coa (see paper)
30% identity, 54% coverage: 8:215/384 of query aligns to 3:195/258 of 1mj3A
- active site: A68 (≠ G71), M73 (≠ G84), S83 (≠ F100), L85 (≠ T102), G109 (= G128), E112 (≠ G131), P131 (= P150), E132 (= E151), T137 (≠ L156), P139 (= P158), G140 (≠ D159)
- binding hexanoyl-coenzyme a: K26 (= K28), A27 (≠ S29), L28 (= L30), A30 (= A32), A66 (= A69), G67 (= G70), A68 (≠ G71), D69 (= D72), I70 (≠ V73), G109 (= G128), P131 (= P150), E132 (= E151), L135 (≠ I154), G140 (≠ D159)
Sites not aligning to the query:
6yswA E. Coli anaerobic trifunctional enzyme subunit-alpha in complex with coenzyme a
31% identity, 50% coverage: 19:210/384 of query aligns to 13:194/707 of 6yswA
- active site: A66 (≠ G71), I71 (≠ L76), A84 (≠ G88), Q88 (≠ D92), G112 (= G128), E115 (≠ G131), P136 (= P150), E137 (= E151), G145 (≠ D159)
- binding coenzyme a: E23 (≠ K28), M25 (≠ L30), A66 (≠ G71), D67 (= D72), I68 (≠ V73), P136 (= P150), E137 (= E151), L140 (≠ I154)
Sites not aligning to the query:
2hw5C The crystal structure of human enoyl-coenzyme a (coa) hydratase short chain 1, echs1
29% identity, 51% coverage: 19:215/384 of query aligns to 17:197/260 of 2hw5C
- active site: A68 (≠ G71), M73 (≠ G84), S83 (≠ F100), L87 (≠ Y104), G111 (= G128), E114 (≠ G131), P133 (= P150), E134 (= E151), T139 (≠ L156), P141 (= P158), G142 (≠ D159)
- binding crotonyl coenzyme a: K26 (= K28), A27 (≠ S29), L28 (= L30), A30 (= A32), K62 (= K65), I70 (≠ V73), F109 (≠ M126)
Sites not aligning to the query:
6slbAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
32% identity, 51% coverage: 21:215/384 of query aligns to 15:194/257 of 6slbAAA
- active site: Q64 (≠ G71), F69 (≠ E83), L80 (≠ F100), N84 (≠ Y104), A108 (≠ G128), S111 (≠ G131), A130 (≠ P150), F131 (≠ E151), L136 (= L156), P138 (= P158), D139 (= D159)
- binding (~{E})-6-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-6-oxidanylidene-hex-3-enoic acid: R58 (≠ K65), A62 (= A69), Q64 (≠ G71), D65 (= D72), L66 (≠ M80), Y76 (≠ A96), A108 (≠ G128), F131 (≠ E151), D139 (= D159)
Sites not aligning to the query:
Q08426 Peroxisomal bifunctional enzyme; PBE; PBFE; L-bifunctional protein; LBP; Multifunctional enzyme 1; MFE1; EC 4.2.1.17; EC 5.3.3.8; EC 1.1.1.35 from Homo sapiens (Human) (see 5 papers)
30% identity, 54% coverage: 25:231/384 of query aligns to 18:206/723 of Q08426
- V40 (≠ A49) to G: in dbSNP:rs1062551
- I41 (≠ V50) to R: in dbSNP:rs1062552
- T75 (= T101) to I: in dbSNP:rs1062553
- K165 (≠ D191) modified: N6-acetyllysine; alternate; mutation to Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-171; Q-346 and Q-584.
- K171 (≠ H197) modified: N6-acetyllysine; mutation to Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-165; Q-346 and Q-584.
Sites not aligning to the query:
- 3 E → K: in FRTS3; the mutant is mistargeted to mitochondria; results in impaired mitochondrial oxidative phosphorylation and defects in the transport of fluids across the epithelium of renal proximal tubular cells; dbSNP:rs398124646
- 274 A → T: in dbSNP:rs2302819
- 325 A → G: in dbSNP:rs1062555
- 346 modified: N6-acetyllysine; K→Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-165; Q-171 and Q-584.
- 584 modified: N6-acetyllysine; alternate; K→Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-165; Q-171 and Q-346.
- 598 K → T: in dbSNP:rs1042437
- 606 T → P: in dbSNP:rs1042438
5jbxB Crystal structure of liuc in complex with coenzyme a and malonic acid (see paper)
28% identity, 48% coverage: 17:200/384 of query aligns to 13:185/261 of 5jbxB
- active site: A67 (≠ G71), R72 (= R78), L84 (≠ T101), R88 (= R105), G112 (= G128), E115 (≠ G131), T134 (≠ P150), E135 (= E151), I140 (≠ L156), P142 (= P158), G143 (≠ D159)
- binding coenzyme a: S24 (≠ K28), R25 (≠ S29), R26 (≠ L30), A28 (= A32), A65 (= A69), D68 (= D72), L69 (≠ V73), K70 (≠ V74), L110 (≠ M126), G111 (= G127), T134 (≠ P150), E135 (= E151), L138 (≠ I154), R168 (≠ Q183)
Sites not aligning to the query:
6slaAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
30% identity, 51% coverage: 21:215/384 of query aligns to 12:182/245 of 6slaAAA
- active site: Q61 (≠ G71), L68 (≠ F100), N72 (≠ Y104), A96 (≠ G128), S99 (≠ G131), A118 (≠ P150), F119 (≠ E151), L124 (= L156), P126 (= P158), N127 (≠ D159)
- binding ~{S}-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyl] 2-(2,5-dihydrooxepin-7-yl)ethanethioate: L21 (= L30), A59 (= A69), Q61 (≠ G71), D62 (= D72), L63 (≠ V73), L68 (≠ F100), Y71 (≠ E103), A94 (≠ M126), G95 (= G127), A96 (≠ G128), F119 (≠ E151), I122 (= I154), L124 (= L156), N127 (≠ D159)
Sites not aligning to the query:
Q62651 Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial; EC 5.3.3.- from Rattus norvegicus (Rat) (see paper)
26% identity, 46% coverage: 24:200/384 of query aligns to 71:247/327 of Q62651
- D176 (≠ G131) mutation D->A,D: Strongly decreases dienoyl-CoA and trienoyl-CoA isomerase activity.
- E196 (= E151) mutation E->D,Q: Strongly decreases dienoyl-CoA and trienoyl-CoA isomerase activity.
- D204 (= D159) mutation D->A,N: Strongly decreases dienoyl-CoA and trienoyl-CoA isomerase activity.
3h81A Crystal structure of enoyl-coa hydratase from mycobacterium tuberculosis (see paper)
28% identity, 48% coverage: 18:200/384 of query aligns to 12:180/256 of 3h81A
- active site: A64 (≠ G71), M69 (≠ L76), T79 (≠ F100), F83 (≠ Y104), G107 (= G128), E110 (≠ G131), P129 (= P150), E130 (= E151), V135 (≠ L156), P137 (= P158), G138 (≠ D159)
Sites not aligning to the query:
3q0jC Crystal structure of the mycobacterium tuberculosis crotonase in complex with the inhibitor acetoacetylcoa
29% identity, 48% coverage: 18:200/384 of query aligns to 13:181/255 of 3q0jC
- active site: A65 (≠ G71), M70 (≠ L76), T80 (= T101), F84 (≠ R105), G108 (= G128), E111 (≠ G131), P130 (= P150), E131 (= E151), V136 (≠ L156), P138 (= P158), G139 (≠ D159)
- binding acetoacetyl-coenzyme a: Q23 (≠ K28), A24 (≠ S29), L25 (= L30), A27 (= A32), A63 (= A69), G64 (= G70), A65 (≠ G71), D66 (= D72), I67 (≠ V73), K68 (≠ V74), M70 (≠ L76), F84 (≠ R105), G107 (= G127), G108 (= G128), E111 (≠ G131), P130 (= P150), E131 (= E151), P138 (= P158), G139 (≠ D159), M140 (≠ I160)
Sites not aligning to the query:
3q0gC Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
29% identity, 48% coverage: 18:200/384 of query aligns to 13:181/255 of 3q0gC
- active site: A65 (≠ G71), M70 (≠ L76), T80 (= T101), F84 (≠ R105), G108 (= G128), E111 (≠ G131), P130 (= P150), E131 (= E151), V136 (≠ L156), P138 (= P158), G139 (≠ D159)
- binding coenzyme a: L25 (= L30), A63 (= A69), I67 (≠ V73), K68 (≠ V74), Y104 (≠ I124), P130 (= P150), E131 (= E151), L134 (≠ I154)
Sites not aligning to the query:
Query Sequence
>WP_086509633.1 NCBI__GCF_002151265.1:WP_086509633.1
MTDLAVIFDELPTRDGGRIGIATLNAPKSLNALSLDMARQLDAKLQAWAVDRSIVAVWLE
GSGEKAFCAGGDVVALYRSMTEEGENRGAGRDSLFAETYFTTEYRLDYRIHRYPKPILVW
GDGIVMGGGLGLMAGAARRLVTETTLIAMPEITIGLYPDIGASWFLNRMPPGVGAYLGLT
GAQLNARDALDLGLADHFVPRERRSELLEALGAADYGTRSRRDLQAGVQGVLDEFEARQQ
APAAQVWPLLDHVQALTAQIDAPAAVRRILADARDDAWLAANRKRLEAGSPLSAHLVWCM
LERHRHTSLADAFRDELNLSVQCCRRGDVAEGVRALLIDKDRNPKWQHASVDDVPEADLQ
ALLEPLWSAEEHPLRDLDCGERVR
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory