SitesBLAST

9 C→A: Appears to be unable to inactivate ManR, since ManR does not show any decrease in its DNA-binding activity in the presence of this mutant.
365 modified: Phosphoserine

P46321 Probable licABCH operon regulator; EC 2.7.1.- from Bacillus subtilis (strain 168) (see paper)
28% identity, 88% coverage: 7:144/156 of query aligns to 501:633/641 of P46321

query
sites
P46321

L

L

P

K

E

E

R

L

A

V

L

F

F

F

D

Q

V

E

P

D

G

L

G

R

S

S

K

K

K

E

R

E

V

V

L

I

E

Q

F

F

F

L

S

G

T

Q

F

K

I

V

A

V

Q

E

N

C

T

-

P

-

S

G

L

F

D

A

S

D

Q

E

E

E

V

I

F

I

G

D

R

S

L

I

I

F

G

E

R

R

E

E

R

D

L

M

G

S

S

P

T

T

G

C

I

F

G

G

N

N

G

L

V

V

A

A

I

I

P

P

H
|
H

A

P

R

L

S

V

P

P

H

Q

C

T

H

K

S

T

P

T

V

F

A

W

A

A

F

V

L

C

K

T

L

L

A

K

E

K

P

P

I

I

D

D

F

W

D

E

A

S

I

-

D

-

G

-

E

Q

P

R

V

V

D

Q

L

F

V

V

F

C

V

L

L

L

L

C

V

V

P

E

E

K

E

E

A

N

D

K

D

A

T

D

H

L

L

Q

S

S

L

M

L

Y

S

K

Q

L

V

L

A

G

S

S

V

I

M

L

N

D

D

D

A

P

E

A

T

A

R

M

S

N

Q

Q

L

L

R

I

K

K

C

C

E

R

S

S

Q

Y

R

Q

E

E

L

L

H

S

E

D

H559 (= H67) mutation to G: Increase in activity.

Sites not aligning to the query:

219 H→A: Residual activity.; H→D: Loss of activity.
278 H→A: Loss of activity.; H→E: Loss of activity.
333 H→A: Loss of activity.
392 H→E: Loss of activity.; H→I: Residual activity.

O31644 Transcriptional regulator ManR; Mannose operon transcriptional activator; EC 2.7.1.191 from Bacillus subtilis (strain 168) (see paper)
35% identity, 46% coverage: 51:121/156 of query aligns to 554:621/648 of O31644

query
sites
O31644

R

R

E

E

R

K

L

M

G

S

S

A

T

T

G

N

I

I

G

G

N

S

G

G

V

I

A

A

I

I

P

P

H
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H

A

A

R

N

S

A

P

K

H

F

C

I

H

K

S

Q

P

S

V

A

A

I

A

A

F

I

L

A

K

T

L

L

A

K

E

E

P

P

I

L

D

E

F

W

D

-

A

-

I

-

D

G

G

N

E

E

P

K

V

V

D

S

L

L

V

V

F

F

V

M

L

L

L

A

V

V

P

K

E

H

E

E

A

D

D

Q

D

T

T

M

H

T

L

K

S

Q

L

L

L

F

S

S

Q

E

V

L

A

S

H570 (= H67) mutation to A: No reduction in DNA-binding activity can be observed in the presence of EIIBA-Man. Leads to a nearly constitutive ManR activity.

Sites not aligning to the query:

222 H→A: Reduced DNA binding.
281 H→A: Reduced DNA binding.
334 H→A: Loss of DNA binding.
393 H→A: Loss of DNA binding.
415 C→A: No effect on DNA binding. Results in a weak constitutive ManR activity.

C0H3V2 Mannitol-specific phosphotransferase enzyme IIA component; EIIA; EIII; PTS system mannitol-specific EIIA component from Bacillus subtilis (strain 168) (see paper)
25% identity, 65% coverage: 41:142/156 of query aligns to 36:135/143 of C0H3V2

query
sites
C0H3V2

S

T

Q

E

E

D

V

Y

F

I

G

S

R

K

L

M

I

F

G

E

R

R

E

E

R

E

L

T

G

S

S

S

T

T

G

F

I

M

G

G

N

N

G

F

V

I

A

A

I

I

P

P

H

H

-

G

-

T

-

E

A

A

R

K

S

S

P

E

H

V

C

L

H

H

S
|
S

P

G

V

I

A

-

A

S

F

I

L

I

K

Q

L

I

A

P

E

E

P

G

I

V

D

E

F

Y

D

G

A

-

I

-

D

E

G

G

E

N

P

T

V

A

D

K

L

V

V

V

F

F

V

G

L

I

L

A

V

G

P

K

E

-

A

-

D

N

T

E

H

H

L

L

S

D

L

I

L

L

S

S

Q

N

V

I

A

A

S

I

V

I

M

C

N

S

D

E

A

E

E

E

T

N

R

I

S

E

Q

R

L

L

R

I

K

S

C

A

E

K

S

S

Q

E

R

E

E

D

L

L

S74 (= S75) modified: Phosphoserine

Query Sequence

>WP_086509854.1 NCBI__GCF_002151265.1:WP_086509854.1
MTLENILPPERALFDVPGGSKKRVLEFFSTFIAQNTPSLDSQEVFGRLIGRERLGSTGIG
NGVAIPHARSPHCHSPVAAFLKLAEPIDFDAIDGEPVDLVFVLLVPEEADDTHLSLLSQV
ASVMNDAETRSQLRKCESQRELHERLIDAIRRQSSA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory