SitesBLAST
Comparing WP_086509898.1 NCBI__GCF_002151265.1:WP_086509898.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
8b7sA Crystal structure of the chloramphenicol-inactivating oxidoreductase from novosphingobium sp (see paper)
38% identity, 95% coverage: 5:540/563 of query aligns to 3:457/458 of 8b7sA
- binding flavin-adenine dinucleotide: G11 (= G13), G13 (= G15), S14 (= S16), A15 (= A17), E35 (= E37), A36 (= A38), W47 (= W66), P65 (≠ G84), G67 (= G86), V180 (≠ T226), A214 (= A260), G215 (= G261), A218 (= A264), T270 (≠ S337), Y391 (= Y474), A424 (= A507), I435 (≠ L518), N436 (= N519)
5nccA Structure of fatty acid photodecarboxylase in complex with fad and palmitic acid (see paper)
35% identity, 96% coverage: 5:542/563 of query aligns to 22:578/578 of 5nccA
- active site: R347 (≠ P326), L420 (vs. gap), I421 (vs. gap), S507 (≠ A473), A509 (≠ H475), G552 (= G516), Q553 (≠ N517)
- binding flavin-adenine dinucleotide: G30 (= G13), G32 (= G15), T33 (≠ S16), A34 (= A17), L53 (= L36), E54 (= E37), A55 (= A38), F74 (≠ L59), W80 (= W66), A98 (≠ G84), G100 (= G86), G105 (= G91), S106 (= S92), N110 (= N96), A111 (≠ G97), T112 (≠ M98), L113 (≠ C99), V238 (≠ T226), A278 (= A260), H282 (≠ A264), L286 (≠ I268), N508 (≠ Y474), Q553 (≠ N517), T554 (≠ L518), G555 (≠ N519), V558 (≠ T522)
6yrvAAA structure of fap after illumination at 100k (see paper)
34% identity, 96% coverage: 5:544/563 of query aligns to 6:571/573 of 6yrvAAA
- binding carbon dioxide: R375 (≠ H365), N499 (≠ Y474)
- binding flavin-adenine dinucleotide: G14 (= G13), G16 (= G15), T17 (≠ S16), A18 (= A17), L37 (= L36), E38 (= E37), A39 (= A38), F58 (≠ L59), W64 (= W66), A82 (≠ G84), G89 (= G91), S90 (= S92), N94 (= N96), A95 (≠ G97), T96 (≠ M98), L97 (≠ C99), M191 (≠ V195), V222 (≠ T226), C264 (= C259), A265 (= A260), G266 (= G261), H269 (≠ A264), N499 (≠ Y474), A534 (= A507), Q544 (≠ N517), T545 (≠ L518), G546 (≠ N519)
- binding heptadecane: V377 (≠ L367), G379 (vs. gap), M380 (≠ I369), G386 (= G375), T389 (≠ A378), Y390 (vs. gap), F393 (vs. gap), T408 (vs. gap), Q410 (vs. gap)
A0A248QE08 Fatty acid photodecarboxylase, chloroplastic; CvFAP; EC 4.1.1.106 from Chlorella variabilis (Green alga) (see paper)
34% identity, 95% coverage: 5:539/563 of query aligns to 82:642/654 of A0A248QE08
- TA 93:94 (≠ SA 16:17) binding
- E114 (= E37) binding
- L162 (≠ G88) binding
- S166 (= S92) binding
- NATL 170:173 (≠ NGMC 96:99) binding
- V298 (≠ T226) binding
- C432 (≠ F345) binding
- R451 (≠ H365) binding
- Y466 (vs. gap) binding
- Q486 (vs. gap) binding
- G622 (≠ N519) binding
3ljpA Crystal structure of choline oxidase v464a mutant (see paper)
35% identity, 94% coverage: 5:534/563 of query aligns to 12:527/530 of 3ljpA
- active site: I333 (≠ S347), P377 (≠ V388), N378 (≠ G389), A464 (= A473), H466 (= H475), V509 (≠ G516), N510 (= N517)
- binding dihydroflavine-adenine dinucleotide: G22 (= G15), S23 (= S16), E44 (= E37), A45 (= A38), W71 (= W66), R89 (= R85), A90 (≠ G86), G95 (= G91), C96 (≠ S92), H99 (≠ I95), N100 (= N96), S101 (≠ G97), I103 (≠ C99), A232 (≠ T226), T269 (≠ A260), D273 (≠ A264), Y465 (= Y474), H466 (= H475), D499 (= D506), A500 (= A507), N510 (= N517), P511 (≠ L518), N512 (= N519), V515 (≠ T522)
4mjwA Crystal structure of choline oxidase in complex with the reaction product glycine betaine (see paper)
35% identity, 94% coverage: 5:534/563 of query aligns to 12:527/532 of 4mjwA
- active site: I333 (≠ S347), P377 (≠ V388), N378 (≠ G389), V464 (≠ A473), H466 (= H475), V509 (≠ G516), N510 (= N517)
- binding flavin-adenine dinucleotide: G20 (= G13), G22 (= G15), S23 (= S16), E44 (= E37), A45 (= A38), W71 (= W66), R89 (= R85), A90 (≠ G86), G95 (= G91), C96 (≠ S92), H99 (≠ I95), N100 (= N96), S101 (≠ G97), I103 (≠ C99), R231 (≠ T225), A232 (≠ T226), T269 (≠ A260), G270 (= G261), D273 (≠ A264), Y465 (= Y474), H466 (= H475), A500 (= A507), N510 (= N517), P511 (≠ L518), N512 (= N519), V515 (≠ T522)
2jbvA Crystal structure of choline oxidase reveals insights into the catalytic mechanism (see paper)
35% identity, 94% coverage: 5:534/563 of query aligns to 12:527/527 of 2jbvA
- active site: I333 (≠ S347), P377 (≠ V388), N378 (≠ G389), V464 (≠ A473), H466 (= H475), V509 (≠ G516), N510 (= N517)
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-5-[(4aS,10aR)-7,8-dimethyl-2,4-dioxo-1,3,4,4a,5,10a-hexahydrobenzo[g]pteridin-10(2H)-yl]-2,3,4-trihydroxypentyl dihydrogen diphosphate: G22 (= G15), S23 (= S16), E44 (= E37), A45 (= A38), W71 (= W66), A90 (≠ G86), G95 (= G91), C96 (≠ S92), H99 (≠ I95), N100 (= N96), S101 (≠ G97), I103 (≠ C99), R231 (≠ T225), A232 (≠ T226), T269 (≠ A260), G270 (= G261), D273 (≠ A264), V464 (≠ A473), Y465 (= Y474), H466 (= H475), D499 (= D506), A500 (= A507), N510 (= N517), P511 (≠ L518), N512 (= N519), V515 (≠ T522)
4ha6A Crystal structure of pyridoxine 4-oxidase - pyridoxamine complex (see paper)
32% identity, 93% coverage: 8:530/563 of query aligns to 3:501/508 of 4ha6A
- active site: F360 (≠ V388), G361 (= G389), H444 (≠ A473), H446 (= H475), G487 (= G516), P488 (≠ N517)
- binding flavin-adenine dinucleotide: G8 (= G13), G10 (= G15), S11 (= S16), A12 (= A17), E32 (= E37), A33 (= A38), W58 (= W66), R77 (= R85), G78 (= G86), G83 (= G91), S84 (= S92), L87 (≠ I95), H88 (≠ N96), A89 (≠ G97), M90 (= M98), G91 (≠ C99), V218 (≠ T226), A251 (= A260), G252 (= G261), E255 (≠ A264), H445 (≠ Y474), A478 (= A507), P488 (≠ N517), I489 (≠ L518), H490 (≠ N519)
- binding 4-(aminomethyl)-5-(hydroxymethyl)-2-methylpyridin-3-ol: A89 (≠ G97), S314 (≠ F345), H444 (≠ A473), H446 (= H475)
3t37A Crystal structure of pyridoxine 4-oxidase from mesorbium loti
32% identity, 93% coverage: 8:530/563 of query aligns to 3:501/509 of 3t37A
- active site: F360 (≠ V388), G361 (= G389), H444 (≠ A473), H446 (= H475), G487 (= G516), P488 (≠ N517)
- binding flavin-adenine dinucleotide: G8 (= G13), G10 (= G15), S11 (= S16), A12 (= A17), E32 (= E37), A33 (= A38), W58 (= W66), R77 (= R85), G78 (= G86), R79 (≠ K87), G83 (= G91), S84 (= S92), H88 (≠ N96), A89 (≠ G97), G91 (≠ C99), R217 (≠ T225), V218 (≠ T226), A251 (= A260), E255 (≠ A264), H445 (≠ Y474), A478 (= A507), P488 (≠ N517), I489 (≠ L518), H490 (≠ N519)
E4QP00 5-(hydroxymethyl)furfural oxidase; 5-hydroxymethylfurfural oxidase; HMFO; Thiol oxidase; EC 1.1.3.47; EC 1.8.3.- from Methylovorus sp. (strain MP688) (see paper)
33% identity, 94% coverage: 7:533/563 of query aligns to 6:527/531 of E4QP00
- V101 (≠ I95) mutation to H: Abolishes activity.
- M103 (≠ G97) mutation to A: 16-fold reduction in catalytic efficiency on vanillyl alcohol.
- V367 (≠ G383) mutation to K: 1.6-fold reduction in catalytic efficiency on vanillyl alcohol. Shows significantly improved activity on the aldehyde 5-formyl-2-furancarboxylate, which results in a better 5-hydroxymethylfurfural to 2,5-furandicarboxylate conversion.; mutation to R: 1.4-fold reduction in catalytic efficiency on vanillyl alcohol. Shows significantly improved activity on the aldehyde 5-formyl-2-furancarboxylate, which results in a better 5-hydroxymethylfurfural to 2,5-furandicarboxylate conversion. Displays a catalytic efficiency toward 5-formyl-2-furancarboxylate that is over 1000-fold higher than that for wild-type; when associated with F-466.
- W369 (≠ Q385) mutation to A: 7.5-fold reduction in catalytic efficiency on vanillyl alcohol.
- V465 (≠ A473) mutation to A: 18-fold reduction in catalytic efficiency on vanillyl alcohol.
- W466 (≠ Y474) mutation to A: 39-fold reduction in catalytic efficiency on vanillyl alcohol. In contrast to wild-type, is active on secondary alcohols, such as (S)-1-phenylethanol, and is strictly enantionselective as this mutant has no activity on (R)-1-phenylethanol. Shows increased activity on the aldehyde 5-formyl-2-furancarboxylate.; mutation to F: 3.4-fold reduction in catalytic efficiency on vanillyl alcohol. In contrast to wild-type, is active on secondary alcohols, such as (S)-1-phenylethanol, and is strictly enantionselective as this mutant has no activity on (R)-1-phenylethanol. Shows increased activity on the aldehyde 5-formyl-2-furancarboxylate. Displays a catalytic efficiency toward 5-formyl-2-furancarboxylate that is over 1000-fold higher than that for wild-type; when associated with R-367.
- H467 (= H475) mutation to A: Abolishes activity.
- N511 (= N517) mutation to A: 53-fold reduction in catalytic efficiency on vanillyl alcohol.
4udqA Crystal structure of 5-hydroxymethylfurfural oxidase (hmfo) in the reduced state
33% identity, 94% coverage: 7:533/563 of query aligns to 2:523/525 of 4udqA
- active site: L331 (≠ F345), F364 (= F384), W365 (≠ Q385), V461 (≠ A473), H463 (= H475), A506 (≠ G516), N507 (= N517)
- binding flavin-adenine dinucleotide: G8 (= G13), G10 (= G15), T11 (≠ S16), A12 (= A17), E32 (= E37), A33 (= A38), W64 (= W66), G88 (= G86), G93 (= G91), G94 (≠ S92), N98 (= N96), M99 (≠ G97), V101 (≠ C99), V229 (≠ T226), T261 (≠ C259), A262 (= A260), W462 (≠ Y474), H463 (= H475), A497 (= A507), N507 (= N517), T508 (≠ L518), N509 (= N519), T512 (= T522)
8bxlB Patulin synthase from penicillium expansum (see paper)
31% identity, 94% coverage: 5:535/563 of query aligns to 12:588/590 of 8bxlB
- binding flavin-adenine dinucleotide: G20 (= G13), G22 (= G15), T23 (≠ S16), A24 (= A17), E44 (= E37), A45 (= A38), W80 (= W66), G100 (= G86), G105 (= G91), S106 (= S92), R109 (≠ I95), N110 (= N96), Y111 (≠ G97), A113 (≠ C99), L253 (≠ T225), A254 (≠ T226), A288 (= A260), Q292 (≠ A264), F525 (≠ Y474), D559 (= D506), A560 (= A507), H570 (≠ N517), P571 (≠ L518), Q572 (≠ N519), L575 (≠ T522)
7vzsA Fad-dpendent glucose dehydrogenase complexed with an inhibitor at ph7.56
30% identity, 94% coverage: 7:534/563 of query aligns to 4:563/566 of 7vzsA
- binding D-glucal: Y6 (= Y9), L22 (= L25), N25 (≠ D28), Y51 (≠ A53), I349 (= I328), Q356 (≠ L335), E411 (≠ H382), E444 (= E420), W445 (≠ K421), K448 (≠ Q424), R499 (≠ E471), N501 (≠ A473), H546 (≠ N517), K563 (≠ R534)
- binding flavin-adenine dinucleotide: G10 (= G13), G12 (= G15), T13 (≠ S16), S14 (≠ A17), E34 (= E37), A35 (= A38), Y51 (≠ A53), F55 (≠ Y57), W61 (= W66), R79 (≠ G84), G81 (= G86), G86 (= G91), T87 (≠ S92), N91 (= N96), G92 (= G97), M93 (= M98), A94 (≠ C99), T232 (= T225), A233 (≠ T226), A273 (= A260), G274 (= G261), R277 (≠ A264), F502 (≠ Y474), A536 (= A507), H546 (≠ N517), L547 (= L518), V548 (≠ N519), L551 (≠ T522)
Sites not aligning to the query:
4yntA Crystal structure of aspergillus flavus fad glucose dehydrogenase (see paper)
30% identity, 94% coverage: 7:534/563 of query aligns to 5:564/570 of 4yntA
- active site: V342 (vs. gap), F413 (≠ G383), W414 (≠ F384), N502 (≠ A473), H504 (= H475), G546 (= G516), H547 (≠ N517)
- binding dihydroflavine-adenine dinucleotide: G13 (= G15), T14 (≠ S16), S15 (≠ A17), E35 (= E37), A36 (= A38), F56 (≠ Y57), W62 (= W66), R80 (≠ G84), G82 (= G86), G87 (= G91), T88 (≠ S92), N92 (= N96), G93 (= G97), M94 (= M98), A95 (≠ C99), A234 (≠ T226), A274 (= A260), R278 (≠ A264), F503 (≠ Y474), A537 (= A507), H547 (≠ N517), L548 (= L518), V549 (≠ N519), L552 (≠ T522)
4ynuA Crystal structure of aspergillus flavus fadgdh in complex with d- glucono-1,5-lactone (see paper)
30% identity, 94% coverage: 7:534/563 of query aligns to 4:563/569 of 4ynuA
- active site: V341 (vs. gap), F412 (≠ G383), W413 (≠ F384), N501 (≠ A473), H503 (= H475), G545 (= G516), H546 (≠ N517)
- binding flavin-adenine dinucleotide: G12 (= G15), T13 (≠ S16), S14 (≠ A17), E34 (= E37), A35 (= A38), Y51 (≠ A53), F55 (≠ Y57), W61 (= W66), R79 (≠ G84), G81 (= G86), G86 (= G91), T87 (≠ S92), N91 (= N96), G92 (= G97), T232 (= T225), A233 (≠ T226), A273 (= A260), G274 (= G261), R277 (≠ A264), F502 (≠ Y474), A536 (= A507), H546 (≠ N517), L547 (= L518), V548 (≠ N519), L551 (≠ T522)
- binding D-glucono-1,5-lactone: Y51 (≠ A53), E411 (≠ H382), A496 (≠ E468), N497 (≠ H469), R499 (≠ E471), R499 (≠ E471), N501 (≠ A473), H503 (= H475), H546 (≠ N517)
Q3L245 Pyranose dehydrogenase 1; PDH 1; Pyranose:quinone oxidoreductase 1; EC 1.1.99.29 from Leucoagaricus meleagris (Western flat-topped agaric) (Agaricus meleagris) (see 2 papers)
28% identity, 93% coverage: 6:527/563 of query aligns to 40:591/602 of Q3L245
- N100 (≠ A67) modified: carbohydrate, N-linked (GlcNAc...) asparagine
- H128 (≠ I95) modified: Tele-8alpha-FAD histidine
- N344 (= N297) modified: carbohydrate, N-linked (GlcNAc...) asparagine
- H537 (= H475) active site, Proton acceptor
- H581 (≠ N517) active site
Sites not aligning to the query:
4h7uA Crystal structure of pyranose dehydrogenase from agaricus meleagris, wildtype (see paper)
28% identity, 93% coverage: 6:527/563 of query aligns to 15:566/577 of 4h7uA
- active site: A343 (≠ K327), V426 (≠ G389), Y510 (≠ A473), H512 (= H475), A555 (≠ G516), H556 (≠ N517)
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-2,3,4-trihydroxy-5-[(4aR)-4a-hydroxy-7,8-dimethyl-2,4-dioxo-3,4,4a,5-tetrahydrobenzo[g]pteridin-10(2H)-yl]pentyl dihydrogen diphosphate (non-preferred name): G22 (= G13), G24 (= G15), T25 (≠ S16), A26 (= A17), E46 (= E37), A47 (= A38), W74 (= W66), G99 (= G91), C100 (≠ S92), H103 (≠ I95), N104 (= N96), G105 (= G97), V107 (≠ C99), L242 (≠ A224), V243 (≠ T225), G282 (≠ A260), G283 (= G261), A286 (= A264), H512 (= H475), A546 (= A507), H556 (≠ N517), T557 (≠ L518), Q558 (≠ N519), V561 (≠ T522)
5i68A Alcohol oxidase from pichia pastoris (see paper)
26% identity, 94% coverage: 6:533/563 of query aligns to 5:631/662 of 5i68A
- active site: H566 (= H475)
- binding flavin-adenine dinucleotide: G13 (≠ A14), G14 (= G15), S15 (= S16), S16 (≠ A17), E37 (= E37), A38 (= A38), M58 (≠ L59), A86 (≠ G86), G91 (= G91), G92 (≠ S92), N96 (= N96), F97 (≠ G97), M99 (≠ C99), K225 (≠ T225), V226 (≠ T226), C267 (≠ A260), G268 (= G261), S271 (≠ A264), W565 (≠ Y474), L605 (≠ A507), N615 (= N517), T616 (≠ L518), Y617 (≠ N519)
5hsaA Alcohol oxidase aox1 from pichia pastoris (see paper)
26% identity, 94% coverage: 6:533/563 of query aligns to 5:631/662 of 5hsaA
- active site: H566 (= H475)
- binding arabino-flavin-adenine dinucleotide: G14 (= G15), S15 (= S16), S16 (≠ A17), E37 (= E37), A38 (= A38), A86 (≠ G86), G92 (≠ S92), N96 (= N96), F97 (≠ G97), M99 (≠ C99), K225 (≠ T225), V226 (≠ T226), C267 (≠ A260), S271 (≠ A264), W565 (≠ Y474), L605 (≠ A507), N615 (= N517), T616 (≠ L518), Y617 (≠ N519)
3q9tA Crystal structure analysis of formate oxidase (see paper)
27% identity, 94% coverage: 7:534/563 of query aligns to 7:570/577 of 3q9tA
- active site: A335 (vs. gap), D422 (vs. gap), A508 (= A473), H510 (= H475), C552 (≠ G516), R553 (≠ N517)
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl(2R,3S,4S)-5-(8-formyl-7-methyl-2,4-dioxo-3,4-dihydrobenzo[g]pteridin-10(2H)-yl)-2,3,4-trihydroxypentyl dihydrogendiphosphate: G15 (= G15), T16 (≠ S16), E37 (= E37), A38 (= A38), W65 (= W66), T85 (≠ G84), R86 (= R85), G87 (= G86), G92 (= G91), S93 (= S92), N97 (= N96), Y98 (≠ G97), F99 (≠ M98), T100 (≠ C99), S229 (≠ A224), S265 (≠ C259), Q266 (≠ A260), E270 (≠ A264), F509 (≠ Y474), D542 (= D506), A543 (= A507), R553 (≠ N517), I554 (≠ L518), Q555 (≠ N519), V558 (≠ T522)
Query Sequence
>WP_086509898.1 NCBI__GCF_002151265.1:WP_086509898.1
MSQIREFDYVIIGAGSAGNVLAARLTEDADVSVLLLEAGGPDYRFDFRTQMPAALAYPLQ
GKRYNWAFETDPEPHMNGRRMECGRGKGLGGSSLINGMCYIRGNALDYDNWASLAGLSDW
RYADCLPYFRKAECRDTGANAYHGGDGPVHVTTPKAGNNELYRAFVDAGVQAGYPETEDV
NGYQQEGFGPMDRFVTPKGRRSSTARGYLDQAKGRDNLTIETHATTDVIEFEGKRAVGVR
YLRRGQTQQVRARREVLLCAGAIASPQILLRSGIGNPAHLREFDIPVVHELPGVGENLQD
HLEMYIQYECKQPITLYPALKWFNQPKIGAEWLLLGSGVGASNQFESCAFIRTHDAEPWP
NLQYHFLPIAISYNGKSAVQAHGFQAHVGSMRSESRGRIRLTSRDPKAAPSILFNYMSRE
KDWQEFRDAIRLTREIIAQPAMDPYRGREISPGPEVESDEQLDAFVREHAETAYHPCGSC
RMGEGDDAVVDGQGRVHGVEGLRVVDASLFPVIPTGNLNAPTIMLAEKIADRIRGREPLP
ASDAPYFVAGDSPARREPQRRLI
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory